90 results about "Double-flowered" patented technology

Transcription of a gene associated with formation of floral organs is suppressed to produce a sterile plant. A plant cell is transfected with a chimeric gene that includes (i) a coding gene of a transcription factor that promotes expression of a gene associated with formation of floral organs, and (ii) a polynucleotide that encodes a functional peptide that converts an arbitrary transcription factor into a transcription repressor, and a chimeric protein in which the transcription factor is fused with the functional peptide is expressed in the plant cell. The expression of the gene associated with formation of floral organs is dominantly suppressed by the chimeric protein, and as a result a male sterile plant is produced that cannot properly form pollen. The chimeric protein also suppresses expression of a gene associated with dehiscence of anther, and as a result a plant is produced in which dehiscence of anther is suppressed. Further, the chimeric protein suppresses expression of target genes of a transcription factor associated with formation of stamen and pistil, and as a result a double flowered plant is produced.

A new and distinct variety of blackberry plant lacking anthocyanin coloration in it's various plant parts and having yellow fruit is described. The new variety named 'Clark Gold' also has double flowers and enlarged sepals. 'Clark Gold' is a sport of the southern dewberry, Rubus trivalis, and is a biennial, thorny, trailing-vine plant type. The yellow fruit is of medium size and has the sweetness of its wild blackberry relative. It is also a very early variety with low chill requirements.

The invention discloses a field management method for nectarine planting, and belongs to fruit planting technique. The field management method comprises the steps of: fertilizer and water management, pruning in growth season, tip control and flower promotion, flower thinning and fruit thinning and disease, insect and pest control. In fertilizer and water management, in-time additional fertilizing and watering are carried out according to fertilizer requirement characteristics of nectarine in each growth season, nitrogen is mainly supplemented in early stage, and phosphorus and potassium are mainly supplemented in late stage; during pruning in the growth season, reasonable pruning is carried out to culture high-yield tree shape; in the tip control and flower promotion, nutrients are inhibited to promote flower bud differentiation, control new shoot growth, promote flowering, save nutrients and improve percentage of fertile fruit; and in flower thinning and fruit thinning, most of flower buds on slim and fragile branches, excess double flower buds on long and medium fruit branches and hypogenetic buds flowering late are thinned when the flower buds are plentiful. The field management method has the beneficial effects that: management is easy, nectarine has high yield and early matures, and the nectarine quality and yield are high.

Transcription of a gene associated with formation of floral organs is suppressed to produce a sterile plant. A plant cell is transfected with a chimeric gene that includes (i) a coding gene of a transcription factor that promotes expression of a gene associated with formation of floral organs, and (ii) a polynucleotide that encodes a functional peptide that converts an arbitrary transcription factor into a transcription repressor, and a chimeric protein in which the transcription factor is fused with the functional peptide is expressed in the plant cell. The expression of the gene associated with formation of floral organs is dominantly suppressed by the chimeric protein, and as a result a male sterile plant is produced that cannot properly form pollen. The chimeric protein also suppresses expression of a gene associated with dehiscence of anther, and as a result a plant is produced in which dehiscence of anther is suppressed. Further, the chimeric protein suppresses expression of target genes of a transcription factor associated with formation of stamen and pistil, and as a result a double flowered plant is produced.

The invention relates to a Chinese rose transcription factor RhMYB4 and application thereof to the regulation and control of floral organ development. Specifically, the invention provides an RhMYB4 gene; the gene is characterized by comprising coding region nucleotide sequences shown as 263 bp to 1039 bp of SEQ ID NO. 23. The invention further provides protein which is coded by the gene or has anamino acid sequence shown as SED ID NO. 24. The invention further provides application of the gene to the regulation of the floral organ development of plants and a low-temperature-resisting capability. Based on the function of the transcription factor RhMYB4 which is found at present, the gene can be applied to the floral organ development of the plants, such as double flower petals, heteromorphosis of the flower petals, stamen abortion and the regulation of the low-temperature-resisting capability.

The invention relates to a double-flower beauty cream. The beauty cream comprises the following ingredients in parts by weight: 0.5 to 13 parts of peach blossom, 3 to 15 parts of chrysanthemum, 15 to 30 parts of brown sugar, 10 to 20 parts of milk, and 5 to 30 parts of honey. The beauty cream is prepared by soaking the peach blossom and the chrysanthemum in parts by weight into clear water, decocting for 20 minutes, filtering to remove flower residue, adding the brown sugar and the milk in parts by weight, boiling into thick juice, concentrating into thick paste of which the relative density is 1.25 to 1.35 at the temperature of 60 DEG C, drying, processing into medicinal powder of 120 meshes, adding the honey and stirring uniformly. The double-flower beauty cream has the effects of preventing wrinkles, removing freckles, blocking the sun and preventing skin cracking, has a good effect, and does not have side effect.

The invention discloses a rice double-flower spikelet gene DF1. The rice double-flower spikelet gene DF1 has a genome nucleotide sequence shown as Seq ID NO: 1 and a cDNA (complementary Deoxyribonucleic Acid) nucleotide sequence shown as Seq ID NO: 2; the invention further discloses protein coded by the protein and has an amino acid sequence shown as Seq ID NO: 3; the invention further discloses amutant gene of the gene and protein coded by the mutant gene, a recombinant expression vector and a host cell which contain the gene, and a method for regulating and controlling the quantity of spikelets and flowerlets of gramineous plants by utilizing the gene; the invention further discloses application of the gene and the protein and the like to regulation and control of the quantity and the yield of the spikelets and the flowerlets of the gramineous plants. According to the rice double-flower spikelet gene DF1, one DF1 gene which influences deterministic development of the spikelets of rice is separated by utilizing a map-based cloning technology and functions of the gene are identified by utilizing a transgene complementary test; the spikelet number of the rice can be improved through a molecular design of the quantity of the flowerlets in the spikelets and novel concept and method are provided for culturing high-yield new varieties.

The invention discloses a double-flower pea date cake, prepared from the following raw materials by weight: 10-20 parts of pea mud, 15-25 parts of traditional Chinese medicine composite date mud, 10-20 parts of flour, 1-2 parts of gelatin, 10-15 parts of honey,10-20 parts of corn flour, 0.1-0.2 part of compressed yeast, 4-8 parts of soya bean powder and a proper amount of water. The cake provided by the invention has mellow taste and fine mouthfeel, and is beneficial for human body health; fried black sesame has stronger fragrance; towel gourd flower and pea flower are fried with salt to effectively sterilize and prolong preservation time.

The invention relates to the field of fermentation and brewing and particularly discloses rose wine. The rose wine is prepared from, by mass, 1 part of petals of double-pedal red roses in a first stage of each year, 40-75 parts of water and 0.0615-0.11475 part of fermentation strains. The color of the prepared rose wine is light red or pink, the alcoholic strength is 7-13 %vol, and the total sugarcontent is 4.1-45 g / L. The preparation method of the rose wine comprises the following steps of (1) selection of raw materials, wherein the petals of the double-pedal red roses in the first stage ofeach year are selected as the raw materials; (2) preparation of fermented mash; (3) blending; (4) pre-fermentation; (5) post-fermentation; (6) aging; (7) primary filter pressing; (8) stability treatment, wherein the wine is placed at 55-56 DEG C for heating for 30 minutes first, then the temperature of the wine is maintained at 50-52 DEG C for 6 hours, and finally, the wine is cooled and then frozen at - 3 DEG C for 20 days; (9) secondary filter pressing; (10) sterilizing and filling. The preparation method solves the problems that existing rose wine has poor fragrance and luster after brewing, and the clarity and stability of the existing rose wine are low.

A medicine for treating breast cancer, which is prepared by mixing iron tree leaves, hedyotis diffusa, lobelia, double flower, toosendan and gynostemma into powder of 100-120 meshes, putting them into capsules, and sterilizing Sterilization produces a capsule product. The medicine of the invention has the effects of warming yang and strengthening the body, soothing the liver and relieving stagnation, clearing heat and detoxifying, can be used for the treatment of breast cancer, and has the characteristics of reliable curative effect, convenience, low medicine price and safe taking. According to clinical observation, the effective rate is 77%.

The embodiment of the invention provides a double-flower attack resistance processing method and device based on a block chain. The method comprises the following steps of: obtaining a sample; each processing service node in the target block chain acquires the double-flower transaction information initiated by the transaction initiation node; determining any one piece of transaction information from the double-flower transaction information as transaction confirmation information; and sharing broadcast information corresponding to the first transaction information and the second transaction information to other processing service nodes, and finally, determining the confirmation number corresponding to the first transaction information and the second transaction information according to thereceived broadcast information and the sent broadcast information. The processing result is obtained according to the confirmation number, the double-pattern transaction information is confirmed randomly, confirmation does not need to be carried out according to the timestamp, and the purposes of improving transaction processing efficiency, saving server operation resources and reducing cost areachieved.

The invention belongs to the field of plant molecular biology, and particularly relates to a loquat flower organ development related transcription factor gene EjPI and application thereof. The full length of the cDNA sequence of EjPI gene is shown in SEQ ID NO. 1, and the amino acid sequence of the coded protein is shown in SEQ ID NO. 2. The EjPI gene of the invention is expressed only in petals,filaments and anthers, but not in sepals and pistils. The EjPI gene overexpression vector is transformed into wild-type arabidopsis and pi-1 mutant by agrobacterium-mediated inflorescence staining. The results show that overexpression of the EjPI gene in wild-type arabidopsis could make the sepals of arabidopsis valvulae and open; and the overexpression of the EjPI gene in the arabidopsis pi-1 mutant can restore petals and stamens. The transgenic plant material obtained by using the EjPI gene overexpression vector can enable the wild-type angiosperms to achieve sepal petalization and can be used for breeding double petal flowers; and the plant pi mutant restores petals and stamens, and a reproduction process is completed effectively. The gene can be used for plant double flower transformation so as to improve ornamental value and restore stamen organs for cross breeding, and has good application prospect.

The invention discloses a preparation for treating postpartum rheumatism, and the preparation is produced from the following components and auxiliaries: lycopodium clavatum, long-stalked kadsura, eucommia ulmoides, double-flower rattan, pterospermum heterophyllum, citronella, pine stump, mint and camphor. The preparation provided by the invention is mainly used for fuming or steaming, hot compressing, massage and kneading for discomforts such as wind cold exogenous disease, numbness of limb, arthralgia, ache, insomnia, fat, fatigue and the like, thereby stimulating the circulation of the blood and causing the muscles and joints to relax, strengthening the body resistance to eliminate pathogenic factors, penetrating up and down and harmonizing exterior and interior. The preparation has recovery enhancement and health care effects on joint, especially postpartum rheumatism; and the effects of the preparation are better if the preparation is applied in the fuming or steaming manner. Compared with the prior art, the preparation is safe in use, accurate in curative effect, simple in prescription, simple and convenient to fabricate and suitable for industrial mass production.

The invention discloses a method for improving a seed yield of double China aster and belongs to the technical field of flower seed collecting cultivation. The method comprises the steps of seeding and growing seedlings according to local normal sowing time of different areas, and performing hole tray seeding or broadcast sowing; planting the seedlings in a field when each seedling has four leaves and one core in about one month after seeding; and completely shearing tops and top ends of all primary branches when the buds just appear at the top ends of the China aster. Through the treatment, a larger number of flowers can grow out at the positions of axils of the primary branches, the size of each scape is one third or a half of a normal scape, most of the flowers are semidouble, the length of each petal is about one third of the length of a normal petal, and fructification conditions are good. Through the method, a larger number of small semidouble flowers can grow out at the positions of the axils of the primary branches, stamens are exposed outside, pollination by insects is not influenced, the fructification conditions are good, and the seed yield is improved by over 6 times.

The invention relates to the field of plant molecular biology, in particular to an EjAG gene determined by stamen and pistil characteristics of loquat, protein encoded by the EjAG gene and application. The whole length of the cDNA (complementary deoxyribonucleic acid) sequence of the EjAG gene is shown in SEQ ID No.1; the amino acid sequence of the protein encoded by the EjAG gene is shown in SEQID No.2. The EjAG gene is only expressed in the stamen and pistil of the loquat, but not expressed in the leaves, sepal and petals. An expression carrier pBI121-EjAg is guided into an arabidopsis ag-1mutant by an agrobacterium-mediated bud infection method; when the transgenic arabidopsis ag-1 mutant flowers, the number of petals and the number of rings are reduced, and the stamen and pistil arerestored. The EjAG gene has the advantages that the theoretical foundation is laid for the modification of plant flower organs by the EjAG gene of the loquat, and the technical scheme for the modification of plant flower organs by the EjAG gene of the loquat is provided; the EjAG gene of the loquat is over-expressed in the arabidopsis ag-1 mutant, so that the phenotype of double flower is restoredinto the normal phenotype; the application prospect is good.

The present invention provides a seed combination for lawn mixed with flower spots. The seed composition comprises the following seeds in parts by weight: linaria vulgaris 1-3 parts, callistephus chinensis 0-5 parts, tall or dwarf cornflower 0-6 parts, dwarf lavatera arborea 0-5 parts, echium vulgare 0-6 parts, french marigold 0-6 parts, corn poppy 1-3 parts, eschscholzia californica 1-3 parts, nigella damascena 0-3 parts, tall or dwarf zinnia elegans 0-6 parts, plains coreopsis 0-6 parts, gypsophila 0-8 parts, orychophragmus violaceus 3-6 parts, dicranostigma leptopodum 3-6 parts, salvia nemorosa 3-8 parts, gaillardia pulchella 4-8 parts, sneezeweed 0-6 parts, dianthus plumarius 3-6 parts, trifolium repens 0-12 parts, dianthus chinensis 3-5 parts, chrysanthemun leucanthemum 2-5 parts, dichondra repens 0-10 parts, duchesnea indica 2-8 parts, Chinese violet 2-8 parts, potentilla chinensis 1-5 parts, dwarf perennial coreopsis 0-6 parts, tall or dwarf cosmos sulphureus 0-6 parts, rudbeckia hirta 0-5 parts, linum perenne perennial flax 3-6 parts, purple coneflower 0-5 parts, tall or dwarf coreopsis lanceolata 3-6 parts, oenothera biennis 0-5 parts and erysimum auratiacum 0-5 parts. According to the seed combination and the planting method thereof, the lawn mixed with flower spots can achieve effects of long flowering period, long green period, and evergreen ground covers in winter.

The invention belongs to the technical field of gene engineering, and particularly discloses application of a GhGDEF3 gene of gerbera hybrida in double flower formation. The invention firstly discovers that expression of the GhGDEF3 gene in the gerbera hybrida can control the length of a transition flower; during inhibition of the expression of the gene, the double gerbera hybrida can become semidouble, so that the gene has important influence on the formation of double flowers of the gerbera hybrida; a theoretical guidance is provided for the molecular research on the flower type of the gerbera hybrida and the breeding of the gerbera hybrida; the GhGDEF3 gene has an important application value.

The invention relates to a method for cultivating an early blossoming and multiplicate rhododendron. The method comprises the following steps of: selecting rhododendron plants which do not have plant diseases and insect pests, are robust in growth vigor and are in the early flowering period, and are used as a cross breeding parent, namely Hanyin No.2 (female)* holacanthus septentrionalis (male); selecting flowers which are about to bloom in the first 20 days of April, removing petals at about 10 in the morning, leaving pistil only, pollinating and bagging; removing bags after 7 to 10 days, harvesting fruits when the fruits are yellowed in the middle ten-days of November, and sowing by taking pure peat soil as a sowing matrix in a greenhouse in the middle ten-days of December, watering fully before sowing, and covering a film and keeping moisture until seedlings emerge; after the seedlings grow to form 5 to 6 leaves, transplanting to a 72-mesh tray, recovering the seedlings, pinching once, applying 0.2 percent of Huaduoduo balance fertilizer once every 10 to 15 days; and planting in a nutritional bag after 3 to 4 months, and managing conventionally.

The invention provides flower syrup and a preparation method thereof. The preparation method of the flower syrup comprises the following steps: A, selecting raw materials; B, infiltrating; C, fermenting; D, filtering; E, concentrating; F, blending: mixing and blending a flower fermentation broth, a flower concentrated solution, white granulated sugar and high fructose corn syrup, boiling out the mixture at 50 to 60 DEG C until the sugar is sufficiently dissolved, and performing UHT (ultra heat treated) to obtain the flower syrup. According to the preparation method of the flower syrup providedby the invention, probiotic bacteria are utilized to ferment double-flowered roses, roselle, sweet-scented osmanthus, jasmine flowers and chrysanthemums, the fermentation broth is rich in a pluralityof beneficial microorganisms, macromolecular substances in the flowers can be decomposed into small molecular substances, then the fermentation broth is centrifuged and concentrated to obtain the flower concentrated solution, the flower fermentation broth, the flower concentrated solution, the white granulated sugar and the high fructose corn syrup are dissolved in proportion, the prepared syrupcan be directly applied to food fields such as drinks, baking and the like, and the natural aroma and flavor of flower ingredients can be retained.

This invention relates to a spirit against tracheitis composed of the following weight proportions: blue-red: 3-7, double flower: 1-5, orange peduncle: 3-7, safflower: 0.1-1, sugar candy: 2-6, honey: 2-6 and distilled spirit: 90-110. The preparation method includes: taking the above mentioned materials in proportion and putting them in a container to be dipped for 15-30 days to be filtered to get the solution-the health care spirit, which can accelerate blood circulation and bring the medicine to the positions of trachea and bronchia to keep them healthy.

The invention relates to multi-petal rose flower and horseradish tree leaf beef paste and a making method thereof, and belongs to the technical field of food processing. According to the multi-petal rose flower and horseradish tree leaf beef paste, multi-petal rose flowers, horseradish tree leaves and beef are used as main raw materials, through reasonable matching, the made multi-petal rose flower and horseradish tree leaf beef paste is rich in flower fragrance and outstanding in nutrition, has the functions of expelling toxins, nourishing faces, shaping bodies, resisting ageing and preventing cancer, has the effect of allaying tiredness, and is beneficial to human health; besides, the multi-petal rose flower and horseradish tree leaf beef paste disclosed by the invention is balanced in nutrients and bright and beautiful in color, has rich delicate fragrance of flowers and fresh and tender mouth feel, is convenient and practical, and is suitable for taste of various crowds; and besides, the making method of the multi-petal rose flower and horseradish tree leaf beef paste disclosed by the invention is simple and reliable, and easy to popularize and apply.

The invention discloses a traditional Chinese medicine formula for treating infectious bursal disease, wherein each medicine is prepared from the following medicinal materials according to mass ratio:dandelion 180-220g, 180-220g, isatis root 180-220g, double flower 90-110g, Scutellaria baicalensis 90-110g, Phellodendron amurense 90-110g, licorice 90-110 gram, 40-60g patchouli, gypsum 40-60 g. Theformula has the effects of clearing away heat, detoxifying, purging fire and removing steam, and can effectively treat infectious bursal disease of chickens, and the course of treatment is three days. On the first day, the symptoms were alleviated obviously, and the effective rate was 96% in 1-2 courses of treatment. There was no adverse reaction in chickens.

The invention relates to a method for eliminating double flowers in a block chain system. A transaction initiator scans the blockchain system to obtain transaction output pointing to the transaction initiator. The transaction initiator screens out transaction output needing to be spent according to the amount of money of the receiver, signs the transaction output needing to be spent by adopting aprivate key of the transaction initiator, writes signature information into a new transaction, and then sends the new transaction to the blockchain system. A block chain system verifies the signatureinformation after receiving the new transaction, and judges whether double-flower exists or not based on a verification result. The invention also relates to a computer readable storage medium and a blockchain system. According to the method and the device, double-flower attacks can be prevented, so that the safety of the blockchain network is improved.

The invention provides a double-petal barbadoslily cultivation method, which includes collecting and processing seed balls, germinating the seed balls in a basin, moving the basin to a greenhouse witha daytime temperature of 28-32 DEG C and a nighttime temperature of 20-25 DEG C to grow until flowering, harvesting a large amount of double-petal barbadoslily pollen, smearing the double-petal pollen on the stigma of a single-petal barbadoslily to pollinate the single-petal barbadoslily, peeling seeds after harvesting fruits, sowing the seeds until seedlings bear seed balls, planting the seed balls in soil until flowering, and screening plants with double-petal and excellent comprehensive ornamental characteristics. In autumn, bulbs are taken out and the outer three layers of scales are stripped off to serve as explants, the explants are inoculated and cultured to obtain seed balls, and then the seed balls are planted in a greenhouse to obtain double-petal barbadoslily flowers. Using theresults of pollen production induced by high temperature from double-petal barbadoslily petals, after intercrossing with the single-petal barbadoslily, double-petal barbadoslily seed balls with excellent ornamental characteristics are bred, so that the breeding difficulty and breeding cycle of the double-petal barbadoslily are shortened, the cost of the double-petal barbadoslily is reduced, and the market demand is met.

The invention discloses a preparation method of a traditional Chinese medicine for treating upper-elimination type diabetes, which belongs to the technical field of preparation methods of traditional Chinese medicines. At present, infusion or oral medicine is generally used to treat diabetes. Long-term use is prone to drug resistance, and the curative effect is not obvious. The technical scheme of the invention is as follows: take 4.5 grams of Coptidis root, 12 grams of Scutellaria baicalensis, 9 grams of Tianhuafen, 9 grams of Anemarrhena, 12 grams of gypsum, 6 grams of Shegan, 12 grams of brocade lanterns, 9 grams of mountain bean root, 12 grams of green fruit, fig 9 grams, 6 grams of gardenia, 15 grams of double flowers, 12 grams of dandelion, 12 grams of Daqingye, 12 grams of isatidis, 9 grams of Patrinia, 12 grams of raw ground juice, 9 grams of asparagus, 9 grams of Ophiopogon japonicus, white Put 18 grams of chrysanthemum root, 18 grams of reed root, 6 grams of ginger juice and 9 grams of licorice. Soak the above-mentioned traditional Chinese medicines in water, then decoct them with slow fire, and finally make a traditional Chinese medicine liquid for treating upper-elimination diabetes. Its advantages are: after oral administration, the curative effect of the medicine is long-lasting and stable, it is not easy to rebound, and it can relieve diabetes.