The invention relates to the technical field of biological histology, in particular to a preparation method of paraffin section of pear flower tissue, which comprises the following steps: taking out the pear flowers preserved in a 4-5 degree Celsius refrigerator and washing them with distilled water, removing the center of the flower, stamen, calyx, petals, and the 3 mm portion below the base of the stalk to obtain the treated pear flower tissue; putting the obtained pear flower tissue into a FAA fixing solution of a fixing container, and evacuating the fixing container until the FAA fixing solution no longer appears bubbles, fixing 1-2days after evacuating; placing the pear flower tissue obtained after evacuating on a gauze to absorb the FAA fixing solution, and carrying out dehydration,transparency, waxing, embedding, slicing, sticking, dewaxing, dyeing and de-floating treatment in turn. According to the preparation method of paraffin section of pear flower tissue, the problem thatthe traditional paraffin section has no strict requirements on material processing, which causes the tissue to be too large when slicing, thereby having a great influence on the experimental result issolved, and the quality of the paraffin section obtained is high.