Method for obtaining regeneration plants of petunia hybrida by anther culture

A technology for regenerating petunia plants, applied in the agricultural field, can solve the problems of low regeneration rate and no regenerated petunia plants, and achieve the effect of improving breeding efficiency and shortening the period of hybrid breeding
CN102499091AInactive Publication Date: 2012-06-20HANGZHOU ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HANGZHOU ACAD OF AGRI SCI
Publication Date
2012-06-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for obtaining regeneration plants of petunia hybrida by anther culture. The method comprises the following steps of: A) performing pretreatment and disinfecting petunia hybrida buds at low temperature; B) peeling calyx and petals of the buds obtained in the step A) by using tweezers, extracting anther, and after removing capillament of the anther completely, inoculating the anther to an inducing callus culture medium; C) transferring callus obtained by subculture in the step B) to a differential culture medium to differentiate adventitious buds, and when the adventitious buds are between 3 and 5 centimeters, stopping differential culture; and D) transferring the adventitious buds which are obtained in the step C) and are used as plantlets to a rooting culture medium to perform rooting culture until at least three adventitious roots of which the lengths are more than or equal to 2 centimeters grow on the plantlets to obtain seedlings which can be planted outside a bottle, wherein the steps A), B), C) and D) are performed in sterile environment.
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Description

technical field

[0001] The invention belongs to the field of agriculture, and in particular relates to a method for obtaining regenerated plants of petunias through anther culture. Background technique

[0002] Petunia (Petunia hybrida Vilm), also known as Bike eggplant and Ganoderma lucidum peony, is a perennial herb belonging to the genus Petunia in the family Solanaceae. Extensive management is an excellent and important flower bed greening grass flower in my country at present, and it can also be used as potted flowers. Petunia is more heat-resistant and is a commonly used greening flower in summer and autumn in my country. Because petunia has obvious heterosis and strong ornamental value, most petunia seeds currently on the market are F1 generation, and they are mainly imported from abroad. The key to hybrid breeding using hybrid vigor is to obtain pure parental parents (inbred lines) with excellent traits. In order to obtain the inbred line, according to the conventi...

Claims

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