1955 results about "Skin callus" patented technology

This invention relates to improved methods for the production of transgenic cotton plants, comprising cocultivating Agrobacterium cells comprising a DNA fragment of interest operably linked to at least one T-DNA border with cotton embryogenic callus in the presence of a plant phenolic compound.

The present invention relates to a method for visualizing GFP expression in callus, various tissue and organ of the transgenic plants as image and system using the same. The said method needs no other additional genetic product, substrate or cofactor and can detect very simply and quickly GFP expression by using the said system of the present invention consisting of a CCD camera, a light source, band-pass filter and data processing computer, so it provides many advantages for selection of transgenic seeds, for studying of gene expression in the tissue or organ of plants, or for studying of specificity of each development step.

The invention relates to a ginseng adventitious root induced proliferation method, which comprises the following steps: cutting ginseng tissue culture seedlings into small tissue blocks and then inoculating into a solid induced culture medium for inducing to form adventitious roots, cutting the adventitious roots into small adventitious root blocks and then inoculating into a liquid proliferation culture medium for performing proliferation culture of the adventitious roots, wherein the solid induced culture medium and the liquid proliferation culture medium take 1 / 2 MS(-N) culture medium as basic culture mediums and contain 1-10mg / L indolebutyric acid. By utilizing the method provided by the invention, the quantity of the adventitious roots obtained through induction can be obviously increased, the adventitious roots obtained through induction are subjected to proliferation culture, and compared with the method for performing adventitious root induction by utilizing callus tissues, the proliferation rate of the adventitious roots is higher, and the proliferation effect is better.

Compositions and methods include a new strain of Bacillus velezensis having growth promoting activity and activity against plant pathogens. The compositions are useful for benefiting plant growth and / or conferring protection against a pathogenic infection when applied to plant foliage, flowers, fruits, bark, roots, seeds, callus tissue, grafts, cuttings, surrounding soil or growth medium, and soil or growth medium concomitant with sowing seed and planting callus tissue, grafts, and cuttings. The compositions containing the Bacillus velezensis RTI301 strain can be applied alone or in combination with other microbial, biological, or chemical insecticides, fungicides, nematicides, bacteriocides, herbicides, plant extracts, plant growth regulators, and fertilizers. In one example, the Bacillus velezensis RTI301 strain can be delivered to the plant as part of an integrated pest management program, with other microbial or chemical insecticides, fungicides, nematicides, bacteriocides, herbicides, plant extracts, and plant growth regulators.

Devices and methods for gradual displacing of the soft tissues covering bones. The gap developing between the bone and the displaced soft tissue will be filled with bone callus as it is in distraction osteogenesis. The devices and methods allow formation of bone in distraction osteogenesis without cutting a segment of the bone. The devices and methods are particularly useful in dental implantology for vertical ridge augmentation by displacing the periosteal tissue and for sinus lift by displacing the Schneiderian membrane. The devices and methods can also regenerate soft tissue between the bone and the displaced soft tissue.

A patch of low friction materials, such as a film of polytetrafluoroethylene is sized to provide an area of low friction support for a portion of a human body relative to an object such as a shoe or prosthetic socket. The patch can be lined with foam, or preferably, a stretch fabric so that it will fit around irregular contours of the shoe, or socket, or the skin itself. The patches reduce shear trauma in critical load areas. After identifying regions of high loads, the method comprises applying the patches as needed to avoid sores, calluses, blisters and abrasions.

In a callus elongating / regenerative device (1), a plate (3) is provided in a bar-shaped fashion to move in a three-dimensional direction depending on a pre-mutilated bone shape so as to bridge a mutilated bone portion (2). A bone cut end (8) is severed to produce two bone pieces (4) which are held by brackets (5). The brackets (5) are pulled to move along the plate (3) to three-dimensionally extend a callus (9) osteoplastically formed between a severed bone end (10) and a severed end surface (11). Since the brackets (5) are formed to move while surrounding the plate (3), the sizes of the plate (3) and the brackets (5) are such a degree as to be placed in a wound to-be osteoplastically treated. Thus, the callus (9) can be three-dimensionally extended with a most part of the callus elongating / regenerative device (1) retained in the wound.

A sponge rubber foot exfoliation apparatus to remove thick, rough, dry, peeling, flaky skin and calluses from the arches, tops, bottoms, sides, heels and toes of feet evenly gently painlessly, smoothly and softly. The first apparatus consisting of a sponge rubber handheld device will attach to a second apparatus. The second apparatus comprising flexible material with abrasives and sponge rubber material affixed with abrasives to exfoliate feet. A single rigid plastic perimeter ring, double rigid plastic plates and single rigid plastic plate of the second apparatus will attach to and secure the flexible and sponge rubber material with abrasives to the first apparatus.

A patch of low friction materials, such as a film of polytetrafluoroethylene is sized to provide an area of low friction support for a portion of a human body relative to an object such as a shoe or prosthetic socket. The patch can be lined with foam, or preferably, a stretch fabric so that it will fit around irregular contours of the shoe, or socket, or the skin itself. The patches reduce shear trauma in critical load areas. After identifying regions of high loads, the method comprises applying the patches as needed to avoid sores, calluses, blisters and abrasions.

The present invention provides a tissue culture method for cultivation of marine algae, said method comprising the steps of (i) establishing axenic viable algal material by sequential treatment thereof in sterile sea water supplemented with domestic liquid detergent, incubating the treated material, (ii) culturing the axenic explants on agarified medium for induction of callus; (iii) excising and subculturing the calli from the axenic explants on fresh agar plates to obtain differentiated densely pigmented oval or spherical shaped micro-propagules (iv) subculturing the pigmented calli in agarified medium to achieve enhanced somatic embryogenesis and micro-propagule formation in pigmented filamentous callus, (v) transferring the filamentous calli with somatic embryos for morphogenesis and development of young plantlets; and (vi) cultivating algal biomass on a large scale by growing the young plantlets in enclosed perforated polythene bags.

The invention discloses a rapid propagation method of grape grafted seedlings. The winter grape hard branch is taken as a rootstock and a scion. Through an appropriate temperature, the graft union is firstly in thermal synthesis. Afterwards the rootstock surface is heated for the callus of the graft union. A medicament with an proper formula is adopted for the dipping of the injured face to facilitate the healing of the graft union and the wound healing and rooting of the rootstock lower part, in which the step is that the first is the rootstock branch treatment, the second is the ear shoot treatment, the third is the inlaying grafting and connecting of the rootstock branch and ear shoot, as well as the ear opening bandaging, the fourth is the heat wound healing of the graft union, the fifth is the wound healing and rooting of the rootstock opening, the sixth is the transplanting and container transplanting seedling. The invention is simple and practical with convenient operation, short seedling cycle which only needs 1 to 8 months, high survival rate which can be more than 90%, low production cost, strong seedling growth, long growth period, fully aging branches and tendrils and good seedling quality, thereby the mechanical large-scale factory production is facilitated.

The present invention is directed to the introduction of molecules, including nucleic acids, carbohydrates, plant growth regulators and peptides into cells and tissues. The present invention is also directed to media and methods for enhancing embryogenic callus production of elite lines of soybean.

The invention mainly relates to a method for regenerating a plant from camellia callus. The method has the following steps: stripping off the inner and outer seed coats of a camellia fruit seed, and inoculating the seed to a 1 / 2MS culture medium; when a sterile seedling grows above 3 cm, inoculating the soft leaf of the young plant to a callus induction culture medium which is MS + 0.5mg.L 6-BA+1.0mg.L 2,4-D; when the callus grows to get a diameter about 1 cm, shifting the callus to a callus differentiation culture medium which is MS+mg.L 6-BA20+0.1mg.L I BA+ mg.L KT0.1; when an indefinite bud grows to 0.5 cm, carrying out the separation and inoculating to a strong bud culture medium which is MS + 0.2mg.L 6-BA+0.05mg.L NAA; and when a bud stick grows to 4 to 5 cm, cutting off the basal of the bud stick, immersing the basal of the bud stick in 1,000 g.L I BA, and then inoculating to a MW + 0.2mg.L I BA+0.2mg.L NAA culture medium. The method has the advantages that: the method has a simple culture medium recipe, a simple and convenient operating process, short culture time, high regeneration frequency and a high propagation expansion coefficient, and facilitates the large-scale production of rare camellia plants and the realization of the genetic transformation of exogenous genes.

The invention discloses a cocoa tea great parental stock grafting method of cultivation, comprising: (1) the choice of the grafting time: the grafting time is from the late autumn or the early winter to the February of the next year; (2) the choice of parental stock: a 5-15 year-old pu erh tea plant growing in tea garden with broken parental stock and repaired fault plane is selected as the parental stock; (3) the choice of cion of the cocoa tea: the cion of the cocoa tea is the clonal fine variety of cocoa tea and the cion wattle is the lignified wattle born in the last year or the semi-lignified wattle born in the current year; (4) cion cutting: the cut cion is immerged into the auxin accelerating the generation of callus; (5) grafting: the cion is engrafted into the parental stock; (6) bag-covering colligating: the parental stock connected with the cion is colligated by a transparent and enduring plastic bag; (7) an awning is established by a rack to protect; (8) the fallow and the supplement of water; (9) the remove of bag: the plastic bag and a sunshade net can be gradually removed after the cion survives. The method of cultivation of the invention has a high survival rate and a high garden forming speed, with unchanged basic property of cocoa tea, thereby increasing the quality of theanine.

The invention discloses a method for cultivating adventitious root tissue of wild panax ginseng C.A.Mey.. The method comprises the following steps of: (1) induction of callus of wild panax ginseng C.A.Mey.; (2) proliferation of the callus of wild panax ginseng C.A.Mey.; (3) induction of adventitious roots of wild panax ginseng C.A.Mey.; and (4) proliferation of the adventitious roots of wild panaxginseng C.A.Mey. in a liquid medium. The method has rapid proliferation of the adventitious roots, high-content saponin compounds, a simple process, low cost, a high induction rate, good repeatability, a short period and no influence of the natural environment at the same time.

Compositions and methods are provided for a combination of a new strain of Bacillus amyloliquefaciens RTI301 and a new strain of Bacillus subtilis RTI477, the combination having growth promoting activity and activity against plant pathogens. The compositions containing the RTI301 and RTI477 strains are useful for benefiting plant growth and / or conferring protection against a pathogenic infection when applied to plant roots, seeds, callus tissue, grafts, and cuttings. Synergistic results are observed for the combination of the strains, and the combination of strains is useful to increase yield in crops including soybean and corn. The compositions containing the combination of strains can be applied alone or in combination with other microbial, biological, or chemical insecticides, fungicides, nematicides, bacteriocides, herbicides, plant extracts, plant growth regulators, and fertilizers.

The invention belongs to the technical field of plant vegetative propagation, and in particular relates to a cuttage seedling raising method for twigs of prunus maritime. The method solves the problems of cuttage seedlings of prunus maritime of rotten calluses of roots, low rooting rate, low rate of survival, week seedlings and susceptibility to water loss and withering of prunus maritime and strengthens the growth and rejuvenation of the roots, stems and leaves of the seedlings during cuttage through hardening seedlings, improves the rate of survival in wild field planting and lays firm good function fur special planting seedlings of pruns maritime.

The tender branch planting method for goldleaf elm includes the following steps: cutting tender branch in 8-15 cm length as cutting wood in May to September, inserting the lower end of the cutting wood with naphthylacetic acid solution in 450-550 ppm concentration for 8-12 sec, planting into medium comprising vermiculite, perlite and turfy soil in the weight ratio of 1 to 0.8-1.2 to 0.8-1.2 inside an arched shed in an all-weather greenhouse, and maintaining the temperature inside the shed at 25-35deg.c and air humidity of 70-90 % for the cutting wood to grow callus in one week, root in 15 days. The said process has high planting survival rate.

Compositions and methods include a new strain of Bacillus amyloliquefaciens having activity against plant pathogens. The compositions are useful for benefiting plant growth and / or conferring protection against a pathogenic infection when applied to plant foliage, flowers, fruits, bark, roots, seeds, callus tissue, grafts, cuttings, surrounding soil or growth medium, and soil or growth medium concomitant with sowing seed and planting callus tissue, grafts, and cuttings. The compositions containing the Bacillus amyloliquefaciens RTI472 strain can be applied alone or in combination with other microbial, biological, or chemical insecticides, fungicides, nematicides, bacteriocides, herbicides, plant extracts, plant growth regulators, or fertilizers. In one example, the Bacillus amyloliquefaciens RTI472 strain can be delivered to the plant as part of an integrated pest management program, with other microbial or chemical insecticides, fungicides, nematicides, bacteriocides, herbicides, plant extracts, and plant growth regulators.

Protoplasts which regenerate reproducibly in a short time to normal, fertile plants can be regenerated from an auxin-autotrophic genotype of Zea mays (L.). Starting from immature embryos on hormone-free media, an auxin-autotrophic, embryogenic callus is formed on the shoot basis of the seedlings, which callus retains its embryogenic potential over a substantial period of time when subcultured on hormone-free medium. In addition to fully-developed embryos, adventitious embryos are also formed under suitable culture conditions (6-9% of sucrose in the medium). When the sucrose content is reduced to 2-3% and 2,4-dichlorophenoxyacetic acid is added, soft, granular calli are formed which consist of embryogenic cell aggregates (type II callus). After subculturing the type II callus in the form of a cell suspension culture, totipotent protoplasts can be isolated. From these protoplasts, the maize plants according to the invention are regenerated.

The invention discloses a quick propagation method for lycoris chinensis. The method comprises the following steps of: taking embryos of the lycoris chinensis, and inoculating the embryos to an induction medium to induce callus; transferring the callus to an MS (Murashige and Skoog) culture medium containing sucrose, naphthyl acetic acid (NAA) and 6-benzyl aminopurine (6-BA) to induce adventitious buds; transferring the adventitious buds to the MS culture medium containing the sucrose, the NAA and the 6-BA to induce bulblets; transferring the expanded bulblets to an MS culture medium containing agar and NAA, and performing induced rooting under illumination; and transferring the rooted bulbs to a transplanting medium of perlite and humus. According to the method disclosed by the invention, the embryos of the lycoris chinensis are used as explants, the explants are induced to generate the callus, then the adventitious buds and the bulblets are induced, and plant regeneration is finally completed, so that a new path is increased for quick propagation of tissue culture of the lycoris chinensis. The method has high propagation coefficient, millions of bulblets can be produced in one year, and an effective path can be provided for quickly propagating seed bulbs; and the test tube plantlets have high differentiation synchronizing degree and good consistency, and the method has high large-scale production development value and good economic prospect.

Methods for the regeneration of cotton plants are disclosed. The use of novel compositions of media, media additives and new growth conditions during stages of development results in increased frequencies of embryogenesis, embryo maturation and embryo germination. The improved process results in higher production frequencies of transformed cotton plants.

The invention discloses a propagation method of succulent plant leaf cutting secondary rooting, and belongs to the technical field of plant cultivation. On the basis of the existing one-time leaf cutting propagation method, the grown new plants are separated from breeding leaves, existing callus of the new plants are removed, the new plants is coated with a rooting powder and cultured in a mixed matrix of the peat and perlite in the mass ratio of 1:1; and 1-2 months later, finish roots appear on the roots of the new plants, so as to complete the propagation of succulent plant. The method carries out secondary treatment on leaf cutting new plants, so that the leaf cutting succulent plants not easy for rooting can root; the method greatly improves the rooting rate and propagation efficiency of succulent plants leaf cutting; and the propagation method is simple, convenient for implementation and large-scale propagation, does not need much maintenance, and has important economic value.

The invention discloses a method for optimizing a potato isolated culture one-step seedling culture medium. The culture medium makes leaves of a potato test tube plantlet undergo isolated culture to form a seedling in one step. The one-step seedling culture medium takes an MS culture medium as a basal medium, and 6-benzyladenine, naphthyl acetic acid and 2,4-dichlorphenoxyacetic acid with different concentrations and combinations are supplied in 1L of the MS culture medium; adventitious buds and adventitious roots are directly differentiated through callus induction; and the potato regenerated seedling is obtained in one step. The callus obtained by induction in a primary culture medium is unnecessarily inoculated to a differential medium for regenerating the seedling. Compared with an isolated culture multi-step regenerated seedling method, the method of the invention has the advantages of simplified steps, short culture period, high repeatability, and high seedling survival rate.

Skin care hand held device and method for caring for human hands and feet useful for safely removing calluses, cuticles, warts and dead cells from human hands and feet, applying a circular, rounded cutting blade to the human skin is disclosed. The present invention relates to a cylindrical scalpel comprising a tube where the tip cut at a 90-degree angle is sharpened to form a curved circular blade integrated with the shank. The blade portion is formed by the inner wall of the tube and the external wall which is formed as to have a curved shape on the outside rim at the tip of the tube, the hollow bore allows access for application of liquid or gaseous additives or vacuum or other types or energy to be applied directly through the tube. Methods for using the foot care device are also disclosed.

The invention belongs to the technical field of vegetative propagation of plants, in particular to a beach plum root cutting seeding method. By applying the invention, the problems of callus decay of a beach plum cuttage shoot root, low rooting rate, low survival rate and tender seeding which is easy to lose water and then withers are solved. Acclimatization in the process strengthens the growth and development of roots, stems and leaves and rejuvenation in the beach plum cuttage seeding process, remarkably improves the survival rate of field planting and establishes the firm foundation for the cultivation and generalization of the beach plum seeding which is a unique forest fruit.

The invention provides an artificial propagation method of Spathiphylium Floribundum, which not only can maintian high propagation efficiency but also can keep high consistence and feature stability of plants. It includes five steps such as female parent pretreatment, explants selection, original variety production, original variety domestication, multiplication, rooting and transplantation. Rhizome and lateral bud are used as explants, and after disinfection they are inoculated onto a certain medium for inducing callus and adventitious buds, then a complete plant is formed, after induction and multiplication, the breeding speed is accelerated, the evenness is high, a large amount of obtained ramets characteristics of the clonal progeny of original good single plant or block mass are identical.