15results about How to "Improve reproductive efficiency" patented technology

The invention relates to an isolated culture method of functional Chinese softshell turtle ovarian granular cells. The isolated culture method comprises the following steps: S1, collecting ovarian tissues: collecting Chinese softshell turtle ovarian tissues; s2, separating and collecting a granular cell layer; s3, digesting and collecting granular cells; s4, primary culture of granulosa cells: transferring the collected cells into a cell culture bottle containing 4 ml of a complete culture medium for primary culture, wherein the complete culture medium comprises the following main components: a DMEM culture medium, 15% of fetal calf serum, 1% of penicillin streptomycin and 1% of an insulin-transferrin-selenium additive; and S5, subculturing the granular cells. The isolated culture method and the culture medium system provided by the invention are simpler and more time-saving and labor-saving; according to the method, the aromatase CYP19A1 can be expressed normally, the characteristics of the granular cells are kept, the aromatase CYP19A1 can be expressed normally, it is indicated that the in-vitro cultured cells can keep the functionality of the cells, and the in-vitro proliferation capacity of the granular cells is improved to a great extent.

The utility model relates to the technical field of wasp breeding equipment, concretely relates to three -dimensional wasp breeding frame of adjustable interval, including left and right two groups of mutually symmetrical vertical beams, mutually symmetrical between two vertical beams in each group, the inside surface of two vertical beams in each group is fixedly installed with a plurality of slide rails, and the top and bottom of four vertical beams are fixedly installed with reinforcing beams, and the detachable installation of support plate is arranged between the two slide rails that left and right face each other, and the end plate body of support plate is located in the slide rail and is connected with the slide rail between sliding, a plurality of nest frames are fixedly installed on the upper surface of support plate, the nest frame part is provided with a protective screen cover, the end part rod body of slide rail is provided with a clamping hole, the outer sleeve pipe is fixedly installed on the front side plate body of support plate, the plug -in limiting rod is slidably connected in the outer sleeve pipe, and the plug -in limiting rod and the clamping hole are inserted and matched. The utility model can adjust interval, has the function of limiting, and is convenient to use.

The application provides an artificial breeding method of Onychostoma wujiangense, and belongs to the technical field of aquatic animal breeding. The artificial breeding method of Onychostoma wujiangense comprises the steps of parent fish classification, parent fish cultivation, artificial parturition induction, natural insemination, artificial insemination, fertilized egg hatching, larva cultivation and juvenile fish cultivation. The parturition induction agent is reasonably matched, the parturition induction injection time is accurate, the parturition induction rate, the fertilization rate and the hatching rate are improved, and the parent fish damage is small. The application also clearly defines the feeding methods at different stages of seed cultivation, improves the survival rate of Onychostoma wujiangense seed cultivation stage, and lays a technical support for the artificial breeding of the endangered species Onychostoma wujiangense.

The present application relates to the technical field of livestock data processing, in particular to a livestock and veterinary breeding data analysis system, which comprises: a state transition analysis module collects data of the breeding cycle of female livestock, integrates the data of all female livestock to construct a transition probability matrix, predicts the future state transition path of female livestock according to the transition probability matrix, and generates a female livestock state transition evaluation result. In the present application, by analyzing the data of each state of the breeding cycle of female livestock, the state change path of female livestock at different stages is determined, accurate prediction and quantitative evaluation of the state in the breeding cycle are realized, and the formulation of the breeding plan is more accurate. By evaluating the residence time and transition trend of each stage, the best breeding time is predicted, and the overall breeding efficiency is improved. Based on the similarity analysis of behavior data such as activity frequency, body temperature and number of reproductions, individual female livestock is divided into different behavior groups, and more targeted reproduction management strategies are formulated for different groups.

The application discloses a method for constructing a Vietnamese terminalia regenerative system, and comprises the following steps: Vietnamese terminalia stem segment test materials are obtained by grafting seedling saplings with superior branches, semi-lignified stem segments with axillary buds are used as explants, and after disinfection, the explants are inoculated into an induction culture medium to induce axillary buds, then the induced axillary buds are subjected to micro-branch cutting to carry out proliferation and rooting culture, and a large number of Vietnamese terminalia regenerative plants can be obtained. The method has the advantages of high propagation efficiency, high phenotype consistency, good genetic stability and the like, greatly shortens the production cycle of Vietnamese terminalia seedlings, reduces the variation risk of the seedlings, and provides high-quality seedlings for ecological afforestation and landscaping.

The application discloses a Chinese sturgeon breeding behavior prediction method, which comprises the following steps: step 1, obtaining and uniformly formatting Chinese sturgeon breeding field monitoring data; step 2, calculating Chinese sturgeon breeding behavior evaluation indexes; step 3, constructing a Chinese sturgeon breeding behavior evaluation model coupled with multiple factors; step 4, identifying key factors and threshold intervals affecting Chinese sturgeon breeding behavior; and step 5, predicting the evolution law of Chinese sturgeon breeding activities under the comprehensive action of multiple factors and putting forward breeding field operation scheduling and protection and restoration strategies. The application solves the problems of the existing Chinese sturgeon breeding monitoring and evaluation means, such as difficulty in unifying multiple source data, difficulty in quantitatively evaluating breeding behavior, difficulty in identifying key factor thresholds, and insufficient prediction and decision support.

The application discloses a kind of northwest arid region sandy soil small crayfish breeding artificial breeding nest manufacturing method, including artificial breeding nest is formed by the stacking of multiple breeding devices, breeding device includes breeding pipe, the entrance of small crayfish is opened in the side of breeding pipe, and strip-shaped through-hole and multiple flow-through holes are provided in the length direction of breeding pipe;At least two breeding pipes are oppositely arranged, and the two ends of breeding net respectively pass through the strip-shaped through-hole of two breeding pipes, and the breeding net and breeding pipe are fixed by fixing belt;Small crayfish breeding nest is formed by stacking multiple breeding devices;Breeding device is simple to make, and production cost is low, and the structural design of breeding device is suitable for the water environment in northwest arid region, to bring good living environment for small crayfish, will not cause pollution to water environment, can create certain economic benefits for small crayfish breeder;The water retention and stability of the breeding device of the application effectively solve the problems faced by traditional nest in sandy water.

This utility model provides an aquaculture support frame with multiple hanging plates, including a support body, which includes a support frame. The support frame includes two parallel and spaced first rods, which are connected by three second rods. The second rods are spaced along the length of the first rods, and the first and second rods are perpendicularly connected. A float is provided at the connection between the first and second rods, and the float floats on the sea surface. Fixed anchors are connected to both ends of the first rods. Each second rod is connected to an aquaculture plate by a metal rope, the length of which is no more than 1m. The aquaculture plates are arranged from left to right along the arrangement of the second rods as a first aquaculture plate, a second aquaculture plate, and a third aquaculture plate. The first aquaculture plate is a vertically placed aquaculture plate, the second aquaculture plate is a triangularly arranged aquaculture plate, and the third aquaculture plate is a horizontally placed aquaculture plate.

The application discloses a use of hydrogen peroxide (H2O2) in constructing an oxidative stress model of female and male chicken primordial germ cells (PGCs); in the application, the H2O2 can achieve the effect of oxidative stress of the female and male chicken PGCs by increasing the ROS content in the female and male chicken PGCs, inhibiting the T-SOD and CAT enzyme activities and increasing the MDA content; the application verifies the effect of the oxidative stress model established by the H2O2 on the female and male chicken PGCs by screening different concentrations of the H2O2 added into the female and male chicken PGCs and then detecting the apoptosis, reproductive markers, mitochondrial functions and autophagy genes of the cells; in the application, the effect of the oxidative stress model constructed by the H2O2 on the female and male chicken PGCs provides new experimental basis for clarifying the molecular mechanism of oxidative stress causing poultry reproductive damage, and provides new ideas and targets for protecting the PGC functions of poultry and improving the reproductive efficiency by regulating the oxidative stress, autophagy or apoptosis pathways in the future, and provides important research and development basis for the selection of compounds resisting oxidative stress.

The application relates to the fields of molecular biological technology and livestock breeding technology, in particular to a SNP molecular marker related to a total number of piglets character, a detection primer pair, a kit and application of the SNP molecular marker in a method for breeding large white pigs with high number of piglets, the SNP molecular marker is located on a pig 9th chromosome, a physical position is located at chr9:65633096 of a genome version Ensembl Sscrofa11.1, a polymorphism site is C or A, and the C allele is positively correlated with the high number of piglets character; through genotype discrimination, selection breeding can be carried out: an individual with a genotype of C / C is preferentially selected or reserved as a breeding pig, because the total number of piglets has the highest potential, so that the genetic improvement speed of a population breeding performance is accelerated.

The present application relates to a kind of efficient regeneration method with the larch of north China as explant, the method comprises the following steps: sowing, disinfection, induction culture, elongation culture, rooting culture, seedling and transplanting.According to the technical scheme provided by the present application, the medium with specific formula is used, so that the larch of north China shows significant advantages in the process of propagation: high differentiation efficiency, large proliferation coefficient, good rooting rate.In addition, the method also has the characteristics of short plant growth cycle, strong adaptability after transplanting and the like.The seedling after cultivation presents the characteristics of robust and straight, good growth and uniformity.The technical scheme of the present application significantly reduces the seedling culture period, effectively reduces the production cost.By establishing the adventitious bud induction system with the larch of north China as starting material, and successfully obtaining regenerated plant, a new technical approach is provided for the rapid propagation of the larch of north China.Meanwhile, the present application also lays the foundation for the future establishment of efficient larch of north China transgenic system.

The application discloses application of a SNP molecular marker related to white dead fetus of pigs on chromosome 2 of pigs, wherein the site of the SNP molecular marker is rs345752533, and the genotype of the SNP molecular marker is TT, TA or AA. The SNP molecular marker provided by the application is significantly related to the white dead fetus trait of pigs, wherein the white dead fetus incidence of the pig with the SNP molecular marker genotype of TT or TA is significantly lower than that of the pig with the SNP molecular marker genotype of AA. Through breeding the pig with the SNP molecular marker genotype of TT or TA, the white dead fetus incidence can be reduced, and the reproduction efficiency of the sow can be improved.

The invention discloses a cuttage method for disporum cantoniense, and belongs to the technical field of disporum cantoniense cuttage, and the specific cuttage method comprises the following steps: (1) plant pretreatment: applying a magnetic treatment agent to root soil of a disporum cantoniense maternal plant; (2) taking cutting slips: cutting root sections of the pretreated maternal plants as the cutting slips; (3) pretreatment of the cutting slips: disinfecting the cutting slips, then performing magnetic attraction treatment, and then smearing the upper ends of the cutting slips with germination promoting gel to obtain treated cutting slips; (4) cutting and management and protection: disinfecting a cutting medium, thoroughly watering the cutting medium, and cutting the lower end of the treated cutting into the medium to complete cutting; after cuttage, the environment humidity is kept at 75-80%, and the temperature is kept at 25 + / -1 DEG C; carrying out magnetic attraction treatment on the cutting slips by using a magnet every day from the 1st day to the 7th day of cuttage; (5) transplanting: transplanting the seedlings to a field 58-60 days after cuttage is completed. The method solves the problems of underdeveloped root system and low propagation efficiency of the disporum cantoniense cuttage.

The utility model discloses a detection device for ovarian maturity of female tilapia, which relates to the technical field of tilapia cultivation and comprises a measuring box, a piston cylinder, a piston bolt and a water adding mechanism, a sample inlet is arranged on the lateral portion of the measuring box, one end of the piston cylinder is communicated with the sample inlet, and the other end of the piston cylinder is movably connected with the piston bolt. A fish adding opening and a water adding opening are formed in the piston barrel, the water adding opening is communicated with the water adding mechanism, and the water adding mechanism can add water into the piston barrel and measure the volume of the added water; the piston bolt can push the female tilapia mossambica to be detected and water in the piston cylinder into the measuring box and seal the sample inlet; a water measuring pipe is arranged at the top of the measuring box and is used for measuring the total volume of the to-be-measured female tilapia and water in the measuring box, so that the volume of the to-be-measured female tilapia is obtained, and the ovarian maturity of the to-be-measured female tilapia is judged according to the ratio of the body length to the volume of the to-be-measured female tilapia. The device can effectively detect whether ovaries of female tilapia mossambica are mature or not, and the purpose of accurate and efficient breeding is achieved.