72results about How to "Improve reproductive efficiency" patented technology

The invention discloses a method for culturing seawater rotifers by using prawn shell leavening, which comprises the following steps: S1, cleaning prawn shells to obtain a fermentation substrate; s2, dynamically optimizing fermentation conditions by adopting a multi-agent collaborative reinforcement learning algorithm; s3, predicting the optimal completion time of fermentation by adopting a mixed graph convolutional neural network and an adaptive genetic algorithm, and providing dilution suggestions through a spectral analysis technology; s4, dynamically calculating the dilution ratio, and mixing the fermentation liquor with seawater; s5, pouring the diluent into a rotifer culture pond, predicting the health state of the rotifer by adopting a Vision Transform model, and automatically adjusting a nutrition formula or environmental conditions; s6, monitoring the density of the rotifers in real time through a YOLOv5 target detection model and a camera, and keeping the rotifers within a preset density range; and S7, performing intelligent management on waste treatment based on a multi-objective evolutionary algorithm of non-dominated sorting. According to the method, a multi-agent collaborative algorithm and a deep learning technology are adopted, and intelligent culture of seawater rotifers and optimized treatment of wastes are realized.

The invention discloses a method for establishing a cudrania tricuspidata stem efficient regeneration system, and belongs to the technical field of plant tissue culture. The establishment method comprises the following steps: cutting healthy Cudrania tricuspidata current-year twigs into stem segments, reserving a plump bud on each stem segment, carrying out preliminary disinfection, soaking in a disinfectant, carrying out ultrasonic treatment and disinfection, using the disinfected bud as an explant, and carrying out bud induction culture, cluster bud enrichment culture and rooting culture to obtain Cudrania tricuspidata in-vitro regenerated seedlings. By screening the components of the culture medium in each stage and optimizing the in-vitro rapid propagation process of the cudrania tricuspidata, the propagation efficiency of a rapid propagation system is improved, a large number of high-quality seedlings can be produced within a short period of time, the market demand is met, and rapid development of industrialization of the cudrania tricuspidata is promoted.

The invention provides a method for improving the breeding efficiency of braconus splendens, and belongs to the technical field of insect breeding. According to the method, female bees which are 5-day-old, 6-day-old and 7-day-old and have no egg laying experience after eclosion are used for parasitizing third-year-old Asiatic corn borer larvae. And the parasitism rate, the eclosion offspring number and the female bee number of the braconus splendens are obviously improved. The method is carried out in a small space range, so that the encountering probability of the braconus splendens and the hosts is increased, the time of searching for the hosts by the braconus splendens is shortened, and the parasitic rate of parasitic wasps is increased. The method disclosed by the invention has important significance in efficiently breeding the cotesia macrorrhiza by using the Asiatic corn borer larvae indoors and guiding the field to release the cotesia macrorrhiza.

The invention provides a deutzia glabrata hard wood cutting breeding method, and relates to the technical field of vegetative propagation seedling raising, and the method comprises the following steps: (1) collecting 1-2-year-old branches, and trimming thin branches; (2) cutting the branches in the step (1) into cutting slips with the length of 10cm and more than two bud points, flatly cutting upper openings of the cutting slips and obliquely cutting lower openings of the cutting slips, and soaking roots of the cutting slips into a sterilizing solution for sterilization treatment; (3) selecting grass carbon mixed perlite as a cutting medium, putting the cutting medium into a plug tray, and disinfecting the plug tray; (4) soaking the cutting slips obtained in the step (2) in a rooting agent, then inserting the cutting slips into a plug tray substrate, thoroughly watering the cutting slips with a sterilizing solution, and covering the cutting slips with a transparent cover; (5) after cuttage, placing under the condition of 70% shading degree, adjusting the shading degree to be 50% and the temperature to be 10-30 DEG C after new leaves emerge, timely supplementing water after cuttage, sterilizing in combination with a sterilizing solution, gradually opening a transparent cover after 45 days of cuttage, and watering according to conditions; and (6) 60 days after cuttage, transplanting and planting. The method has the advantages that the seedling raising period is shortened, and the breeding efficiency of deutzia glabrata is improved.

The invention belongs to the technical field of molecular biotechnology and molecular markers, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker located on a porcine chromosome 5 and related to a live litter size character and an application of the SNP molecular marker. The SNP site of the SNP molecular marker located on the pig chromosome 5 and related to the live litter size character corresponds to the Tgt at the 89503311bp position on the chromosome 5 of the international pig reference genome version 11.1; g mutation. Whole genome correlation analysis and detection of the molecular marker show that the molecular marker significantly affects the character of the number of live piglets born by pigs. The invention also provides a primer pair and a kit for identifying the molecular marker, an efficient and accurate molecular marker assisted breeding technology can be established by utilizing the molecular marker, the primer pair or the kit, and the molecular marker assisted breeding technology is applied to the character genetic improvement of the live birth number of the boar, so that the live birth number of the boar is increased, the profit of an enterprise is increased, and the core competitiveness is improved.

The invention belongs to the technical field of molecular biology and molecular markers, and discloses an SNP molecular marker related to the number of mature follicles of Holstein cattle, a marker combination and application of the SNP molecular marker. The molecular marker related to the Holstein cattle mature follicle number character is screened on the basis of whole genome association analysis, the molecular marker is utilized, and the number of mature follicles of a group can be remarkably increased through molecular marker-assisted breeding, reservation of individuals with favorable genotypes and elimination of individuals with unfavorable genotypes, so that the number of mature follicles of the group is increased. Therefore, the purpose of improving the breeding efficiency of the excellent Holstein cows is achieved.

The invention relates to an isolated culture method of functional Chinese softshell turtle ovarian granular cells. The isolated culture method comprises the following steps: S1, collecting ovarian tissues: collecting Chinese softshell turtle ovarian tissues; s2, separating and collecting a granular cell layer; s3, digesting and collecting granular cells; s4, primary culture of granulosa cells: transferring the collected cells into a cell culture bottle containing 4 ml of a complete culture medium for primary culture, wherein the complete culture medium comprises the following main components: a DMEM culture medium, 15% of fetal calf serum, 1% of penicillin streptomycin and 1% of an insulin-transferrin-selenium additive; and S5, subculturing the granular cells. The isolated culture method and the culture medium system provided by the invention are simpler and more time-saving and labor-saving; according to the method, the aromatase CYP19A1 can be expressed normally, the characteristics of the granular cells are kept, the aromatase CYP19A1 can be expressed normally, it is indicated that the in-vitro cultured cells can keep the functionality of the cells, and the in-vitro proliferation capacity of the granular cells is improved to a great extent.

The invention discloses a preparation method of a predatory mite feed and a predatory mite feeding method. The predatory mite feed comprises a basic artificial feed and biomass, the basic artificial feed comprises egg yolk liquid, yeast extract powder, cane sugar, honey, purified water, peptone, potassium sorbate and p-hydroxybenzoate; the preparation method comprises the following steps: fully mixing the components into a semi-fluid mixture, and freeze-drying the semi-fluid mixture into a solid state at low temperature by using an instantaneous low-temperature freeze-drying technology to obtain the predatory mite feed. The predatory mite freeze-dried feed is prepared to be used for feeding predatory mites, a traditional feeding scheme of plants-pest mites-predatory mites can be replaced, the whole feeding process is simple, conditions are controllable and easy to control, and the feeding difficulty and the feeding cost are effectively reduced; meanwhile, the predatory mite feed is prepared into a freeze-dried state, the feed utilization rate can be increased, the predatory mites can easily eat the feed, and therefore the breeding efficiency of the predatory mites is improved.

The invention discloses a soapberry SmSEP3 gene and application thereof, the gene is expressed in a plant to regulate and control the flowering time of the plant and promote pistil development, sepal curling and pod development, and the gene is a nucleotide sequence as shown in a sequence table Seq ID NO.1 or a nucleotide sequence of an amino acid sequence as shown in a coding sequence table Seq ID NO.2. The gene is used for cultivating new soapberry varieties with different flowering times, different flower types and larger fruits. Through the analysis of transgenic arabidopsis thaliana, the bolting and flowering time of the plant can be advanced through the overexpression of the arabidopsis thaliana, the pistil is obviously lengthened, sepals are curled, and the fruit volume is larger. The flowering time, bolting days and rosette leaf number of the transgenic arabidopsis thaliana plant are obviously different from those of a wild plant, pistil development is longer, fruit pods are increased, the breeding cycle of the sapindus tree species is accelerated, ornamental varieties of different flower types are cultivated, and cultivation of high-yield new varieties is accelerated.

The invention relates to the technical field of livestock breeding, in particular to a cow breeding system and method based on artificial intelligence, and the system comprises a data collection module, a determination module, a judgment module, a screening module, an adjustment module, a calculation module, a prediction module and a correction module. According to the system, accurate real-time data collection and intelligent analysis are combined, the physiological state of the dairy cow can be comprehensively monitored, and the system not only improves the diagnosis accuracy of the health condition of the dairy cow, but also can dynamically adjust a feeding scheme to optimize the reproduction rate by integrating multiple parameters such as hair density, glossiness, body temperature and skin moisture, so that the breeding efficiency is improved. In addition, due to the introduction of the artificial intelligence model, the prediction of the reproductive rate is more accurate, and by correcting the threshold value and the reproductive coefficient, the problem that the reproductive condition of a dairy cow group cannot be accurately and comprehensively reflected due to the unstable measurement of a traditional sensor and the limitation of individual monitoring is effectively solved.

The invention provides a fish welfare type method for improving the breeding efficiency of wild snakehead, and belongs to the technical field of aquaculture, the method has significant advantages in the aspect of wild snakehead breeding, when parent fish is caught, specific water areas and opportunities are selected, the parent fish is transported with water by gently taking and releasing, parent fish injury is reduced, and the survival rate is increased. According to parent fish cultivation, different aquatic plants are cultivated, the vegetation area proportion and the reasonable stocking density are controlled, a suitable environment is provided for parent fishes, special treatment is conducted in the wintering period in the northern area to guarantee survival of the parent fishes, specification and age requirements are defined in the spawning induction link, cost reduction and efficiency improvement are promoted, and the spawning induction rate is increased in a specific spawning induction agent injection and matching mode; the weight ratio of male and female parent fishes is controlled to facilitate mating and spawning, the parent fishes are timely recovered for continuous breeding after delivery, the loss of the wild parent fishes is further reduced, water changing and oil film removing are achieved through an innovative egg collecting method, fertilized egg development is facilitated, the method is friendly to the parent fishes, and the breeding efficiency is comprehensively improved.

The utility model relates to the technical field of wasp breeding equipment, concretely relates to three -dimensional wasp breeding frame of adjustable interval, including left and right two groups of mutually symmetrical vertical beams, mutually symmetrical between two vertical beams in each group, the inside surface of two vertical beams in each group is fixedly installed with a plurality of slide rails, and the top and bottom of four vertical beams are fixedly installed with reinforcing beams, and the detachable installation of support plate is arranged between the two slide rails that left and right face each other, and the end plate body of support plate is located in the slide rail and is connected with the slide rail between sliding, a plurality of nest frames are fixedly installed on the upper surface of support plate, the nest frame part is provided with a protective screen cover, the end part rod body of slide rail is provided with a clamping hole, the outer sleeve pipe is fixedly installed on the front side plate body of support plate, the plug -in limiting rod is slidably connected in the outer sleeve pipe, and the plug -in limiting rod and the clamping hole are inserted and matched. The utility model can adjust interval, has the function of limiting, and is convenient to use.

The application provides an artificial breeding method of Onychostoma wujiangense, and belongs to the technical field of aquatic animal breeding. The artificial breeding method of Onychostoma wujiangense comprises the steps of parent fish classification, parent fish cultivation, artificial parturition induction, natural insemination, artificial insemination, fertilized egg hatching, larva cultivation and juvenile fish cultivation. The parturition induction agent is reasonably matched, the parturition induction injection time is accurate, the parturition induction rate, the fertilization rate and the hatching rate are improved, and the parent fish damage is small. The application also clearly defines the feeding methods at different stages of seed cultivation, improves the survival rate of Onychostoma wujiangense seed cultivation stage, and lays a technical support for the artificial breeding of the endangered species Onychostoma wujiangense.

The present invention relates to the field of animal husbandry technology, and in particular to a dairy cow breeding system and method based on artificial intelligence, the system comprising: a data acquisition module, a determination module, a judgment module, a screening module, an adjustment module, a calculation module, a prediction module and a correction module. The present invention can comprehensively monitor the physiological state of dairy cows by combining accurate real-time data acquisition with intelligent analysis. The system not only improves the diagnostic accuracy of the health status of dairy cows by integrating multiple parameters such as hair density, glossiness, body temperature and skin moisture, but also can dynamically adjust the feeding plan to optimize the reproduction rate and reduce health problems caused by improper feeding. In addition, the introduction of artificial intelligence models makes the prediction of the reproduction rate more accurate. By correcting the threshold and the reproduction coefficient, the problem of being unable to accurately and comprehensively reflect the reproduction status of dairy cow groups due to the unstable measurement of traditional sensors and the limitations of individual monitoring is effectively solved.

The present application relates to the technical field of livestock data processing, in particular to a livestock and veterinary breeding data analysis system, which comprises: a state transition analysis module collects data of the breeding cycle of female livestock, integrates the data of all female livestock to construct a transition probability matrix, predicts the future state transition path of female livestock according to the transition probability matrix, and generates a female livestock state transition evaluation result. In the present application, by analyzing the data of each state of the breeding cycle of female livestock, the state change path of female livestock at different stages is determined, accurate prediction and quantitative evaluation of the state in the breeding cycle are realized, and the formulation of the breeding plan is more accurate. By evaluating the residence time and transition trend of each stage, the best breeding time is predicted, and the overall breeding efficiency is improved. Based on the similarity analysis of behavior data such as activity frequency, body temperature and number of reproductions, individual female livestock is divided into different behavior groups, and more targeted reproduction management strategies are formulated for different groups.

The application discloses a method for constructing a Vietnamese terminalia regenerative system, and comprises the following steps: Vietnamese terminalia stem segment test materials are obtained by grafting seedling saplings with superior branches, semi-lignified stem segments with axillary buds are used as explants, and after disinfection, the explants are inoculated into an induction culture medium to induce axillary buds, then the induced axillary buds are subjected to micro-branch cutting to carry out proliferation and rooting culture, and a large number of Vietnamese terminalia regenerative plants can be obtained. The method has the advantages of high propagation efficiency, high phenotype consistency, good genetic stability and the like, greatly shortens the production cycle of Vietnamese terminalia seedlings, reduces the variation risk of the seedlings, and provides high-quality seedlings for ecological afforestation and landscaping.

The application discloses a Chinese sturgeon breeding behavior prediction method, which comprises the following steps: step 1, obtaining and uniformly formatting Chinese sturgeon breeding field monitoring data; step 2, calculating Chinese sturgeon breeding behavior evaluation indexes; step 3, constructing a Chinese sturgeon breeding behavior evaluation model coupled with multiple factors; step 4, identifying key factors and threshold intervals affecting Chinese sturgeon breeding behavior; and step 5, predicting the evolution law of Chinese sturgeon breeding activities under the comprehensive action of multiple factors and putting forward breeding field operation scheduling and protection and restoration strategies. The application solves the problems of the existing Chinese sturgeon breeding monitoring and evaluation means, such as difficulty in unifying multiple source data, difficulty in quantitatively evaluating breeding behavior, difficulty in identifying key factor thresholds, and insufficient prediction and decision support.

The utility model provides a leech culture pond with a water circulation function, and relates to the technical field of aquaculture, the leech culture pond comprises a culture pond and an adjusting and purifying mechanism, one side of the culture pond is provided with the adjusting and purifying mechanism, and the adjusting and purifying mechanism is arranged through the arrangement of an adjusting and cooling mechanism. An operator sets a water temperature threshold value in advance through the man-machine interaction panel according to the optimal breeding temperature requirement of leeches, the temperature sensor monitors and feeds back temperature information of pond water in real time, and once it is detected that the current water temperature is lower than the preset lowest appropriate temperature threshold value, the control box immediately responds to the signal, the water bottom heater is started, and the water bottom heater is started. And otherwise, when the temperature sensor detects that the water temperature in the culture pond exceeds a threshold value, the control box also commands the motor to drive the baffle to be opened and the electric fan to start to work to help the water body to dissipate heat, so that the water temperature is reduced and is kept in a stable range suitable for the growth and reproduction of the leeches.

The invention belongs to the technical field of plant cultivation, and particularly relates to a Mongolian medicine plant Artemisia stelleriana cutting propagation method, which comprises the following steps: S1, disinfecting Artemisia stelleriana branches; s2, pruning the disinfected Artemisia stelleriana branches, and performing disinfection again to obtain Artemisia stelleriana cuttage segments; s3, treating the cutting sections of the Artemisia stelleriana by using a rooting agent, and cutting the cutting sections of the Artemisia stelleriana subjected to rooting treatment in a matrix for cutting propagation. The cutting propagation method of the Mongolian medicine plant Artemisia stelleriana shows remarkable beneficial effects in the aspects of ecological environment protection, propagation efficiency improvement, germplasm resource enrichment, stress resistance enhancement, economic benefit improvement and the like, and has important popularization and application values.

The invention relates to the technical field of poplar cutting seedling raising, in particular to a poplar ultra-short branch cutting seedling raising method which comprises the following steps: (1) selecting poplar ultra-short branches with buds with the length of 3-5cm and the diameter of 0.3-0.5 cm, soaking and then performing surface disinfection; (2) obliquely inserting the treated ultra-short branches into a special matrix at an angle of 60-70 degrees, wherein the insertion depth is 2 / 3 + / -5% of the length of the branches; (3) placing the seedling raising plate after cuttage in an environment with the temperature of 25 + / -2 DEG C and the relative humidity of 80-90%, and spraying 2-3 times every day; (4) providing illumination by adopting an LED growth lamp; (5) after 14 days of cuttage, foliage spraying is carried out; the survival rate is increased to 90% or above and is increased by 50-60% compared with that of a traditional method, and the seedling raising cost is greatly reduced; the root activity is improved by 139%, the quantity is increased, the quality is also remarkably improved, and a solid foundation is laid for later-stage growth of plants.

The invention discloses a method for inducing coral larva discharge and a high-adhesion-rate larva culture method. And slowly adding the high-salinity seawater into the seawater containing the coral cervi, and inducing the coral cervi to discharge the plantlet larvae. According to the method, high-salinity seawater is used for inducing the coral antler to discharge the planula larvae, so that the damage of a long-time high-temperature induction method to the coral parent and symbiotic zooxanthellae is remarkably reduced, the risks of whitening and death of the coral are avoided, and the survival rate and the recovery speed of the coral parent are increased.

The application discloses a kind of northwest arid region sandy soil small crayfish breeding artificial breeding nest manufacturing method, including artificial breeding nest is formed by the stacking of multiple breeding devices, breeding device includes breeding pipe, the entrance of small crayfish is opened in the side of breeding pipe, and strip-shaped through-hole and multiple flow-through holes are provided in the length direction of breeding pipe;At least two breeding pipes are oppositely arranged, and the two ends of breeding net respectively pass through the strip-shaped through-hole of two breeding pipes, and the breeding net and breeding pipe are fixed by fixing belt;Small crayfish breeding nest is formed by stacking multiple breeding devices;Breeding device is simple to make, and production cost is low, and the structural design of breeding device is suitable for the water environment in northwest arid region, to bring good living environment for small crayfish, will not cause pollution to water environment, can create certain economic benefits for small crayfish breeder;The water retention and stability of the breeding device of the application effectively solve the problems faced by traditional nest in sandy water.

This utility model provides an aquaculture support frame with multiple hanging plates, including a support body, which includes a support frame. The support frame includes two parallel and spaced first rods, which are connected by three second rods. The second rods are spaced along the length of the first rods, and the first and second rods are perpendicularly connected. A float is provided at the connection between the first and second rods, and the float floats on the sea surface. Fixed anchors are connected to both ends of the first rods. Each second rod is connected to an aquaculture plate by a metal rope, the length of which is no more than 1m. The aquaculture plates are arranged from left to right along the arrangement of the second rods as a first aquaculture plate, a second aquaculture plate, and a third aquaculture plate. The first aquaculture plate is a vertically placed aquaculture plate, the second aquaculture plate is a triangularly arranged aquaculture plate, and the third aquaculture plate is a horizontally placed aquaculture plate.

The application discloses a use of hydrogen peroxide (H2O2) in constructing an oxidative stress model of female and male chicken primordial germ cells (PGCs); in the application, the H2O2 can achieve the effect of oxidative stress of the female and male chicken PGCs by increasing the ROS content in the female and male chicken PGCs, inhibiting the T-SOD and CAT enzyme activities and increasing the MDA content; the application verifies the effect of the oxidative stress model established by the H2O2 on the female and male chicken PGCs by screening different concentrations of the H2O2 added into the female and male chicken PGCs and then detecting the apoptosis, reproductive markers, mitochondrial functions and autophagy genes of the cells; in the application, the effect of the oxidative stress model constructed by the H2O2 on the female and male chicken PGCs provides new experimental basis for clarifying the molecular mechanism of oxidative stress causing poultry reproductive damage, and provides new ideas and targets for protecting the PGC functions of poultry and improving the reproductive efficiency by regulating the oxidative stress, autophagy or apoptosis pathways in the future, and provides important research and development basis for the selection of compounds resisting oxidative stress.

The utility model discloses an oviposition collecting device suitable for large-scale breeding of cantheconidea furcellata, which comprises a nymph breeding net cage, a connecting pipe, an adult breeding net cage, an oviposition suspension line, an oviposition frame, a feed basin and oviposition cloth, one end of the connecting pipe is communicated with the nymph breeding net cage, and the other end of the connecting pipe is communicated with the adult breeding net cage; the oviposition suspension line is arranged at the top of the adult feeding net cage, and the oviposition frame and the feed basin are arranged in the adult feeding net cage; and the spawning cloth is suspended on the spawning suspension line and / or the spawning frame. The device has the advantages that the difficulty of manual pairing and manual egg taking can be greatly reduced, the labor cost in the breeding process is remarkably reduced, meanwhile, the frequency of manual water replenishing is reduced, the breeding efficiency and the egg taking efficiency are remarkably improved, and the device is very suitable for large-scale breeding and egg taking of cantheconidea furcellata. The resource guarantee is provided for further improving the biological control effect and efficiency.

The invention discloses a rapid seedless grape breeding method in the technical field of plant cultivation, which comprises the following steps: step 1, selecting male and female parents and hybridizing: selecting seedless excellent varieties as female parents and male parents respectively, performing artificial inflorescence emasculation on the female parents by using tweezers, bagging and marking, and performing artificial pollination; step 2, embryo rescue: shearing and taking back the hybridized clusters, washing the fruits with running water for 2-4 hours, transferring the fruits into a bottle for disinfection, stripping ovules, inoculating the taken ovules into an embryo development culture medium for dark culture, and after 8-10 weeks, cutting off the embryos and inoculating the embryos into an embryo germination culture medium for light culture; and step 3, transplanting and seedling hardening. The method is simple and easy to operate, through the design of the functional scissors, a sample is disinfected and coated with a culture solution while shearing is achieved, then the sample is disinfected, the activity of the sample is kept, and a good foundation is laid for subsequent seedless grape cultivation.

The invention discloses a twig cutting propagation method for a crape myrtle color leaf type variety 'Fengjin', and belongs to the technical field of crape myrtle cutting propagation. The method comprises the steps of cutting slip collection and treatment, matrix treatment, cutting, later-stage management and protection, seedling hardening and the like, and by strictly controlling the cutting matrix and a rooting agent, managing after cutting and the like, the crape myrtle color leaf type variety 'Fengjin' is obtained. According to the method, good rooting and survival of the'paijin 'lagerstroemia indica are promoted, seedlings which grow well are obtained, the survival rate after transplanting is increased, and the method has important significance in promoting application and popularization of a new variety of lagerstroemia indica'paijin'.

The application relates to the fields of molecular biological technology and livestock breeding technology, in particular to a SNP molecular marker related to a total number of piglets character, a detection primer pair, a kit and application of the SNP molecular marker in a method for breeding large white pigs with high number of piglets, the SNP molecular marker is located on a pig 9th chromosome, a physical position is located at chr9:65633096 of a genome version Ensembl Sscrofa11.1, a polymorphism site is C or A, and the C allele is positively correlated with the high number of piglets character; through genotype discrimination, selection breeding can be carried out: an individual with a genotype of C / C is preferentially selected or reserved as a breeding pig, because the total number of piglets has the highest potential, so that the genetic improvement speed of a population breeding performance is accelerated.

The invention discloses sludge reduction equipment with a special structure for strengthening stable attachment of worms, the sludge reduction equipment comprises a box body, a worm stuffing box, a porous conveyor belt, a sludge inlet assembly, a water inlet assembly, an aeration assembly and a drainage assembly, the worm stuffing box is connected with a fixing plate through a connecting rod, the fixing plate is provided with a mounting rod, the mounting rod is connected to the box body, and the porous conveyor belt is arranged on the mounting rod. Roll shafts are arranged on the periphery of the fixing plate in a penetrating mode, roller wheels are fixedly arranged on the four roll shafts in a sleeving mode, the four roller wheels are in transmission connection through a linkage belt, a plurality of hole grooves are formed in the porous conveying belt, a plurality of holes are evenly formed in the hole grooves, the porous conveying belt is installed on the roller wheels in a tensioning mode, and driving assemblies are in transmission connection with the roll shafts. The sludge inlet assembly and the water inlet assembly are both arranged above the porous conveyor belt, and the aeration assembly and the drainage assembly are both installed in the box body. According to the invention, efficient utilization and long-term stable operation of the worms are realized, the dispersibility and adhesion density of the worms are improved, and efficient and stable treatment of sewage sludge is realized.

The invention discloses a kind of efficient propagation method of Codonopsis pilosula, by using culture medium A in bottle to complete callus induction, then using culture medium B to complete the induction and proliferation steps of adventitious buds, the proliferation coefficient reaches 45.0-50.0;Then cooperate with "micro-cutting" outside the bottle, so that the rooting rate of adventitious buds reaches more than 90%, and subsequent domestication and transplanting steps can be smoothly carried out, and then after continuing to grow and grow, the rooting seedlings with thick roots and strong seedlings are obtained, and the seedling hardening and transplanting are carried out, and the survival rate after transplanting is also more than 90%. The present invention not only simplifies the tissue culture production process of Codonopsis pilosula seedlings, and the proliferation coefficient is much higher than the existing reports of other species of the same family, and the efficient "micro-cutting" rooting system combines adventitious root induction and domestication, greatly shortens the seedling raising cycle of Codonopsis pilosula tissue culture seedlings, solves the low proliferation coefficient in the tissue culture process of Codonopsis pilosula plants in Campanulaceae, the phenomenon such as difficult rooting and domestication, and low cost, short cycle, high quality and survival rate.