The invention relates to the technical field of
stem cell culture, in particular to an AI
algorithm for evaluating the growth state of mesenchymal stem cells, which comprises the following steps: acquiring
mesenchymal stem cell image data, and inputting related basic information; extracting morphological characteristic data of the cells from the image data by utilizing a
stem cell culture technology and a
deep learning algorithm, and judging whether the cells have
pollution, degeneration or abnormal morphology; based on the
cell morphological characteristic data, analyzing the growth stage of the cells, and adjusting culture conditions according to the characteristics of the stage; collecting
epigenetics data of stem cells, predicting
cell proliferation and differentiation trends by using a
deep learning model in combination with
cell morphological characteristic data and
cell growth stage analysis results, and adjusting culture conditions; and monitoring
metabolite data in the
stem cell culture solution in real time, evaluating the health state of the cells by combining the
metabolite data, the cell morphological characteristic data and the
cell cycle analysis result, and judging whether the problem of abnormal
metabolism or decline exists or not.