The invention provides a staple cartridge assembly of a disposable intracavity cutting and edge incising anastomat. The staple cartridge assembly comprises a staple cartridge, a staple cartridge base and a staple butting plate, wherein the staple cartridge is arranged in the staple cartridge base, a cutting slot is formed in the middle of the staple cartridge, multiple rows of suturing staple slots are formed in two sides of the cutting slot in the staple cartridge, staple pushing piece groups corresponding to the suturing staple slots are arranged in the staple cartridge seat below the staple cartridge, tissue pressing block slots are further formed in the staple cartridge on one side of the cutting slot and are located on the staple cartridge between one row of suturing staple slots on the innermost layer and the cutting slot, tissue pressing structures are arranged on the staple pushing piece groups and/or staple butting plate corresponding to the tissue pressing block slots, and the tissue pressing structures are tissue pressing blocks at certain heights. With the adoption of the tissue pressing structures, pathological frozen sections after an operation can be made while complete tissue is reserved, the stability of the tissue during the operation can also be guaranteed, blood vessel breakage is reduced, and the operation risk is reduced.