The invention discloses an adjustable hardness test fixture. A pedestal is arranged on a chassis, a left supporting frame and a right supporting frame are symmetrically installed on both sides of thepedestal, a positioning disk is connected to the tops of the left supporting frame and the right supporting frame, the section of a fixture seat takes the shape of a major arc, a guide groove is formed in the fixture seat, a sliding sleeve is sleeved on the periphery of the middle segment of the fixture seat, an objective table is arranged on the sliding sleeve, a tooth surface clamping block is connected to a left-handed screw, a plane clamping block is connected to a right-handed screw, and adjusting screws are installed on upright posts. By adoption of the adjustable hardness test fixture disclosed by the invention, a tested workpiece of any shape can be pressed and fixed, the operation is stable, the hardness test is met, and the phenomenon that when the hardness test is performed on an irregular tested workpiece, bias pressing is generated due to the unstable fixation, resulting in a large deviation between the measured hardness and the actual hardness, is solved. The working efficiency of inspectors can be greatly improved, the accuracy and stability of the microhardness test are ensured, and the applicability is wide.