The invention provides white spirit with an anti-fatigue function, wherein 0.15g-0.16g of ginseng extract, 0.02g-0.03g of astragalus root extract, 0.10g-0.15g of horny goat weed extract and 2.0g-2.5g of wolfberry extract are added to 33-68%Vol white spirit per liter so that the content of total saponins of panax ginseng is greater than or equal to 60mg/L, the content of icariin is greater than or equal to 20mg/L, the content of astragaloside is greater than or equal to 5mg/L and the content of glycine betaine is greater than or equal to 490mg/L in the white spirit by Re. The white spirit with the anti-fatigue function has the advantages that ginseng, astragalus root, horny goat weed and wolfberry are taken as raw materials and the active ingredients total saponins of panax ginseng, astragaloside, icariin and glycine betaine of the raw materials are extracted and added to the white spirit, respectively, and therefore, the original flavor, color and luster as well as taste of the white spirit are remained, the white spirit is clear in ingredients and stable in content, and meanwhile, the white spirit has accurate anti-fatigue function; the white spirit with the anti-fatigue function is yellowish in color, aromatic and elegant, soft, lasting sweet and smooth, harmonious in various aromas, and smooth and clear in taste; judged by professional wine taster, the white spirit with the anti-fatigue function is close to common kaoliang spirit or pure grain wine in style.