46 results about "Astragaloside" patented technology

The invention relates to the technical field of biological medicines and ophthalmic medicines, in particular to a herbal exosome composition for eye care and treatment and a preparation method thereof. The composition comprises a synergistic combination of herbal exosomes and mesenchymal stem cell exosomes, and further comprises a nano slow-release carrier system. Wherein the herbal exosome is extracted from a traditional herbal plant liquorice by combining gradient centrifugation with a molecular exclusion chromatography method, and herbal active ingredients, namely astragaloside and quercetin, are loaded in the exosome by adopting an electroporation method, so that the pharmacological activity of the exosome is enhanced. The nano slow-release carrier system is of a two-phase slow-release structure; an inner phase is a phospholipid complex loaded with herbal exosomes and mesenchymal stem cell exosomes, and an outer phase is temperature-sensitive hydrogel; the compound is in a liquid state at room temperature, and is rapidly converted into a gel state after being dropped into the ocular surface, so that the retention time of the medicine on the ocular surface is remarkably prolonged, the bioavailability is improved, and the targeting slow release capability aiming at ocular surface tissues is endowed to the compound.

The invention belongs to the technical field of biomedical materials, and particularly relates to astragaloside-loaded conductive hydrogel as well as a preparation method and application thereof. The preparation method comprises the following steps: uniformly mixing a methylacrylic anhydride gelatin aqueous solution with an organic solvent solution of astragaloside, and carrying out ultraviolet light cross-linking curing to form methylacrylic anhydride gelatin / astragaloside hydrogel; soaking the obtained hydrogel in hydrochloric acid containing a free radical initiator; then soaking and polymerizing in an aniline ethanol solution; and soaking and purifying to obtain the methylacrylic anhydride gelatin-polyaniline conductive hydrogel loaded with astragaloside. In-vitro experiments prove that the compound has a certain anti-inflammatory effect, can induce neural stem cells to be directionally differentiated into neurons and oligodendrocytes, and is beneficial to promoting nerve regeneration and myelin sheath formation; finally, in-vivo experiments prove that the composition can improve the athletic ability and tissue function recovery of SCI rats, and has a good application prospect.

The invention provides lactobacillus reuteri GY-18 and application thereof in increasing the content of astragaloside, and belongs to the technical field of microorganisms, fermentation and natural product biotransformation. The lactobacillus reuteri GY-18 capable of improving the content of astragaloside in a fermented astragalus membranaceus product is obtained through screening, the lactobacillus reuteri GY-18 is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC M 20252796, and the lactobacillus reuteri GY-18 can be used for improving the content of astragaloside in the fermented astragalus membranaceus product. The lactobacillus reuteri GY-18 can secrete acetylxylan esterase for promoting conversion of astragaloside into astragaloside IV, has efficient specificity on conversion of astragaloside into astragaloside IV, and is high in conversion capacity; according to the method, the content of astragaloside IV is remarkably increased within 24 hours in a semi-solid state fermentation mode, and the method has good practicability.

The invention belongs to the technical field of biomedical materials. The invention provides a composite nano preparation for treating diabetic ulcer and promoting wound healing and a preparation method thereof. The preparation method comprises the following steps: mixing tetra (4-carboxyl phenyl) porphyrin and FeCl3. 6H2O for reaction, then adding a mixed solution composed of benzoic acid and 3, 5-dicarboxyphenylboronic acid for reaction, and then performing centrifugal drying; dispersing in water, adding a chloroplatinic acid solution, stirring, adding a sodium borohydride solution, continuously stirring, and centrifugally drying; stirring with a mixture of curcumin, flavonoid glycoside and astragaloside in ethanol, and carrying out centrifugal drying; and finally, stirring with glucose oxidase in water, and centrifugally drying. The composite nano preparation provided by the invention has an excellent antibacterial effect on multi-drug-resistant bacteria, can effectively destroy a biological membrane, can well treat diabetic ulcer and promote wound healing, and has a relatively good application prospect.

The invention discloses a method for extracting astragaloside IV from astragalus membranaceus, and relates to the technical field of traditional Chinese medicine extraction.The method comprises the following steps that firstly, astragalus membranaceus powder is pretreated and then put into an extraction kettle of a supercritical CO2 extraction device; in the first stage, an entrainer a is used for extraction for 15-20 min under the conditions that the pressure is 30-35 MPa, the temperature is 50-54 DEG C and the CO2 flow speed is 0.8-0.9 L / min; in the second stage, an entrainer b is used for extraction for 75-85 min under the conditions that the pressure is 28-33 MPa, the temperature is 52-56 DEG C and the CO2 flow rate is 0.6-0.7 L / min; in the third stage, an entrainer c is used for extraction for 15-20 min under the conditions that the pressure is 20-25 MPa, the temperature is 42-46 DEG C and the CO2 flow rate is 0.4-0.5 L / min; and finally collecting the extract and purifying to obtain a pure product of astragaloside. The method for extracting astragaloside IV from astragalus membranaceus, provided by the invention, has relatively high extraction rate and purity.

The invention discloses a method for determining chemical components in a medicine for treating vitiligo, which relates to the technical field of medicine analysis, and is characterized in that ultra-high performance liquid chromatography-mass spectrometry is adopted to determine the content of chemical components in a Bailing tablet: psoralen, isopsoralen, astragaloside, quercetin, polysaccharide and amino acid. The determination method comprises the following steps: respectively injecting a reference solution and a test solution into a liquid chromatograph for determination to obtain a chromatogram, and calculating the content of paeoniflorin in a test sample by adopting an external standard method, chromatographic conditions of a chromatogram obtained by adopting a liquid chromatograph are as follows: octadecylsilane chemically bonded silica is taken as a filling agent. According to the method for determining the chemical components in the medicine for treating leucoderma, the established detection method is verified, and the result shows that the detection method is easy and convenient to operate, accurate in result, high in specificity and good in reproducibility.

The invention relates to glycyrrhizic acid astragaloside composite hydrogel, and belongs to the technical field of medical materials. The invention aims to solve the problems that astragaloside is poor in water solubility, and hydrogel prepared by a traditional method is uneven in drug entrapment and insufficient in healing promoting performance. According to the technical scheme, the preparation method is characterized in that glycyrrhizic acid is subjected to ultrasonic pretreatment to form nano-micelles, astragaloside is entrapped forcibly through high-pressure homogenization, and finally the composite hydrogel with a compact structure is obtained through programmed cooling. The glycyrrhizic acid astragaloside composite hydrogel prepared by the invention is mainly used for preparing dressings or medicines for promoting skin wound healing, and is particularly suitable for treating diabetic wounds.

The invention provides a mesenchymal stem cell cryopreservation solution and a preparation method thereof, and belongs to the technical field of cell engineering. According to the cryopreservation liquid, diluted DMEM is used as a basic culture medium, and trehalose, L-proline, polyethylene glycol, sodium carboxymethyl cellulose, sodium glycinate, sericin, glycerin, resveratrol and other components are introduced as additives on the basis of the basic culture medium. The formula of the cryopreservation liquid adopts a DMSO-free and serum-free scheme, sodium glycinate, sericin and glycerol are used as composite protective agents, ice crystal damage can be effectively relieved, osmotic pressure balance can be maintained, cell membrane and protein structures can be protected, the effect is better than that of DMSO and glycerol, and cytotoxicity is avoided. In addition, resveratrol, coryne, astragaloside and other components are used as growth factors, so that the activity of the mesenchymal stem cells in a cryopreservation state is maintained. The survival rate of the umbilical cord mesenchymal stem cells cryopreserved for 2 years can reach 99.83% at most, the cell viability OD value reaches 0.96, and the cell adherence and growth conditions are good when the cells are observed under a microscope.

The invention discloses an application of a compound composition containing astragaloside IV in preparation of a medicine for resisting plateau fatigue, and the compound composition containing astragaloside IV is prepared from the following raw materials in parts by weight: 500 to 2000 parts of astragaloside IV, 5000 to 20000 parts of ginsenoside Rg1, 2 to 5 parts of vitamin D3 and 30000 to 150000 parts of puerarin. Animal experiments prove that the compound composition can relieve plateau exercise fatigue, improve the hypoxia tolerance, exercise fatigue resistance and oxidative stress resistance of a body and improve the comprehensive exercise performance of the body. According to the invention, the defect of single-component efficacy is overcome, a compound preparation is designed in a targeted manner, an innovative and feasible compound combination is provided, and a new scheme is provided for promoting athletic performance under plateau hypoxia.

The invention discloses a preparation method of a radix astragali extracting solution with high antioxidant activity and the extracting solution, and aims to solve the problems of low extraction rate of active substances and inaccurate detection of antioxidant activity in the prior art. According to the method, five factors including ethanol content, extracting solution multiple and the like are taken as key variables, U10 * (108) uniform test design is adopted, the DPPH free radical scavenging rate is accurately determined by combining an electron paramagnetic resonance technology, and the contents of astragaloside, general flavone and polysaccharide are synchronously detected. A quantitative model is established through multivariate linear regression analysis, and the optimal process parameters are determined: the ethanol content is 80%, the extracting solution multiple is 4, the temperature is 85 DEG C, the time is 1.5 h, and the extraction time is 1 time. The DPPH free radical scavenging rate of the extracting solution prepared through the technology is larger than or equal to 92.75%, polysaccharide is larger than or equal to 12520 mg / L, total flavone is larger than or equal to 323.77 mg / L, astragaloside is larger than or equal to 257 mg / L, synergistic efficient extraction of multiple components is achieved, and the extracting solution is suitable for industrial production.

The invention belongs to the technical field of pharmaceutical compositions, and particularly relates to an antibacterial and hemostatic pharmaceutical composition for skin healing and a preparation method thereof. The pharmaceutical composition is double gel and is composed of oleogel and hydrogel according to the mass ratio of (3-5): (5-7), the oleogel takes medium-chain triglyceride and Xiawei wax as matrixes and loads astragaloside, and the hydrogel takes chitosan and sodium alginate as matrixes and loads antibacterial peptide GL13K. The preparation method comprises the following steps: preparing the CS-SA-GL13K hydrogel and the medium-chain triglyceride small single-tree wax-astragaloside oil gel, mixing the CS-SA-GL13K hydrogel and the medium-chain triglyceride small single-tree wax-astragaloside oil gel in proportion, homogenizing and emulsifying, and quickly cooling to obtain the double gel. Tests show that the sterilization rates of the pharmaceutical composition on staphylococcus aureus and escherichia coli are both greater than 95%, the hemostatic effect is good, skin wound healing can be promoted, and the stability is good.

The application relates to application of astragaloside B to preparation of a medicine for treating oligoasthenospermia and the medicine, and belongs to the field of traditional Chinese medicine. Through animal experiments, it is shown that the astragaloside B significantly improves the quantity and quality of rat sperms, and no obvious side effects are observed during the treatment. The astragaloside B has the effects of inhibiting oxidation stress, promoting secretion of testosterone, regulating secretion of follicle stimulating hormone and luteinizing hormone, promoting growth and maturation of sperms, effectively improving sperm function, and enhancing the repair capacity of the body to testicular injury. Moreover, no obvious side effects are observed during the treatment of the astragaloside B on oligoasthenospermia.

The application provides a preparation method of cycloastragenol and relates to the technical field of biology.The preparation method of the cycloastragenol comprises the following steps: preparing a mixed solution of astragaloside, xylosidase and GSM microbial agent, and preparing the cycloastragenol through a conversion reaction.The preparation method can realize 100% conversion of the astragaloside through the synergistic effect of the GSM microbial agent and the xylosidase, and the preparation method is simple and efficient, and the prepared cycloastragenol has high yield and high purity.

The invention relates to a preparation method of a standard astragalus extract, and belongs to the technical field of traditional Chinese medicine extraction. The preparation method comprises the following steps: taking radix astragali decoction pieces, adding alkaline water with the pH value of 12.0-13.0, carrying out reflux extraction for 3-5 times, filtering, merging filtrate, drying the merged filtrate under reduced pressure, freezing, carrying out vacuum drying, and crushing to obtain the standard radix astragali extract. According to the method, key parameters such as pH value, extraction temperature, extraction times and time of alkaline water extraction are controlled, so that efficient extraction and stable preparation of astragaloside and polysaccharide are realized, and a technical basis is provided for fully developing and utilizing an astragalus membranaceus medicinal material and promoting modern application of the astragalus membranaceus medicinal material.

This invention discloses a methane inhibitor based on plant functional components and its application. The inhibitor, by mass fraction (100%), comprises the following raw materials: 1.5-6% p-hydroxybenzoic acid, 0.4-2.4% astragaloside, 5-40% polyethylene glycol 4000, and 81.6-99.999% bentonite. The methane inhibitor provided by this invention uses p-hydroxybenzoic acid and astragaloside as active ingredients. Through their synergistic effect, it effectively inhibits the activity of methanogenic bacteria and the methane production pathway in the rumen of ruminants, while simultaneously improving the rumen fermentation pattern. The above-mentioned methane inhibitor uses natural plant-derived active ingredients, is safe, non-toxic, environmentally friendly, and has a simple preparation process suitable for large-scale production.

The application provides a mesenchymal stem cell cryopreservation solution and a preparation method thereof, and belongs to the technical field of cell engineering. The cryopreservation solution takes diluted DMEM as a basic culture medium, and introduces trehalose, L-proline, polyethylene glycol, sodium carboxymethyl cellulose, sodium glycine, sericin, glycerol, resveratrol and other components as additives. The cryopreservation solution formula adopts a DMSO-free and serum-free scheme, uses sodium glycine, sericin and glycerol as a composite protective agent, can effectively alleviate ice crystal damage, maintain osmotic pressure balance and protect cell membranes and protein structures, and has an effect superior to DMSO and glycerol and no cytotoxicity. In addition, the application uses resveratrol, coenzyme and astragaloside and other components as growth factors to maintain the activity of mesenchymal stem cells in a frozen state. After umbilical cord mesenchymal stem cells are cryopreserved for 2 years according to the application, the survival rate of the umbilical cord mesenchymal stem cells can reach 99.83% at most, the cell viability OD value reaches 0.96, and the cells are observed to have good adhesion and growth conditions under a microscope. △ The survival rate of the umbilical cord mesenchymal stem cells can reach 99.83% at most, the cell viability OD value reaches 0.96, and the cells are observed to have good adhesion and growth conditions under a microscope.

The invention discloses an astragaloside nano preparation as well as a preparation method and application thereof. The preparation method comprises the following steps: S1, dissolving astragaloside in an organic solvent to prepare an astragaloside solution as an organic phase; dissolving a stabilizer in water to prepare a stabilizer solution as a water phase; s2, the astragaloside solution and the stabilizer solution are pumped into a micro-fluidic chip from different channel inlets according to the set total flow velocity and flow velocity ratio, micro mixing is conducted, and an astragaloside nano suspension is prepared; s3, the astragaloside nano suspension is dried, and the astragaloside nano preparation is prepared.

The invention provides a method for extracting astragaloside and quercetin from astragalus mongholicus. The method comprises the following steps: crushing the astragalus mongholicus, performing alcohol extraction and water extraction, filtering, concentrating, performing alcohol precipitation, performing chromatography, crystallizing and recrystallizing to obtain pure products of the quercetin and the astragaloside. Through the steps of water extraction and alcohol precipitation, the interference of astragalus polysaccharide on the separation and purification of astragaloside and quercetin is reduced, the separation and purification steps are effectively shortened, the production efficiency is greatly improved, and the investment of production equipment is reduced; according to the method, an alcohol solution is subjected to alcohol recovery, astragaloside and quercetin can be separated through ethyl acetate extraction, degradation of quercetin caused by acid and alkali treatment is completely avoided, high-content astragaloside and quercetin are obtained through chromatography, crystallization and recrystallization, and the purification cost is also saved.

The invention discloses a process for preparing astragaloside through multi-strain fermentation. Through segmented biotransformation, by utilizing a segmented process that in-situ enzyme production of trichoderma reesei, aspergillus oryzae deacetylation and aspergillus niger step-by-step desugaring are carried out to directionally transform upstream saponin, and bacillus subtilis and candida are used for terminal modification, astragaloside is not subjected to continuous desugaring or ring opening to obtain cycloastragenol after being formed, and the yield of astragaloside is remarkably improved.

The invention discloses a culture method and application of high-secretion IFN-gamma CD4 + T cells, and relates to the technical field of immune cell culture. The cell culture medium disclosed by the invention is prepared from the following components: IL-2, IL-7, IL-15, IL-18, AB plasma, ConA, OKT3, CD28, an STING agonist, astragaloside, ginsenoside Rh2 and astragalus polysaccharide. The CD4 + T cells cultured by the culture medium disclosed by the invention are cultured in vitro for 15 days, the content of the CD4 + T cells is greater than 70%, and the CD4 + T cells secrete IFN-gamma at a higher level compared with the CD4 + T cells cultured by a common CD4 + T cell culture medium, and in addition, the in-vitro killing rate of the CD4 + T cells to HCT116 intestinal cancer cells reaches 95% or above due to the added STING agonist, astragaloside, ginsenoside Rh2 and astragalus polysaccharide.

The invention discloses a traditional Chinese medicine grease oral cream for repairing gastric mucosa and a preparation method thereof, optimizes a traditional heating extraction process into direct compatibility of traditional Chinese medicine extracts, and belongs to the technical field of traditional Chinese medicine preparations. The emulsifiable paste is prepared from a fixed-proportion grease base (20% of emu oil, 40% of olive oil, 20% of coconut oil and 20% of castor oil), six traditional Chinese medicine extract effective components (1-2% of astragaloside, 1-2% of atractylenolide, 1-2% of atractylenone, 1-2% of berberine, 1-2% of scutellaria baicalensis extract and 1-2% of obakunone), 3% of vitamin E, 20-25% of sea buckthorn seed oil and 10% of excipient beewax. According to the preparation process, heating extraction is abandoned, the problems that the extract is difficult to dissolve and easy to agglomerate in grease are solved through infiltrating the extract with a small amount of grease, homogenizing at the low temperature of 60 DEG C and shaping and fixing beewax, and uniform dispersion and stable dispersion of the extract are realized.

The invention relates to the technical field of feed preparation, in particular to an artemisia apiacea chicken feed and a preparation method thereof.According to the artemisia apiacea chicken feed and the preparation method thereof, artemisia apiacea dried and smashed materials with the specific particle size and the artemisinin content being not lower than 0.5% are scientifically compounded with astragalus membranaceus powder with the immunity enhancing function and the astragaloside content being not lower than 0.8%, and dual guarantees of antibiosis, anti-inflammation and immunoregulation are formed; meanwhile, the accurate proportion of trace elements in the premix ensures the metabolic balance of the body, and the various active components have a synergistic effect, so that the premix not only has excellent performance in the aspects of promoting the growth and development of the chickens and improving the feed conversion rate, but also enhances the immune function of the body and optimizes the intestinal health, realizes the comprehensive improvement of the growth performance, disease resistance and intestinal microecology, and improves the immunity of the chickens. In this way, the technical problems that in the prior art, a chicken feed formula is usually single, the function focus point is narrow, and growth, immunity enhancement and intestinal tract improvement are difficult to achieve are solved.

The invention discloses a pharmaceutical composition for cardiovascular and cerebrovascular diseases, and relates to the technical field of traditional Chinese medicines, the pharmaceutical composition comprises the following raw materials by weight: 150-250 parts of panax notoginseng saponins, 8-12 parts of ginkgo total flavonoids, 3-5 parts of dipyridamole, 80-120 parts of a salvianolic acid extract, 5-15 parts of astragaloside, 20-40 parts of a composition of saikoside a and albiflorin, and 30-50 parts of a composition of ginsenoside Rg1 and ophiopogonin D; according to the pharmaceutical composition, the system treatment rule of eliminating, clearing, lowering, warming, harmonizing and tonifying in the traditional Chinese medicine is completely and quantitatively applied to the design of the modern traditional Chinese medicine compound composition for the first time, so that the abstract theory is converted into specific quantifiable components and proportions, and the problem that the traditional compound is not clear and unclear is solved; all the components in the composition are safe components which are recorded in pharmacopoeia or are clinically used for a long time, and potential risks caused by large-dose use of a single component can be reduced through reasonable compatibility and mutual restriction.

The invention provides a preparation method of astragaloside-rADSCs hydrogel, which comprises the following steps: extracting rADSCs, culturing the rADSCs to a P3 generation, co-culturing the rADSCs cultured to the P3 generation and an astragaloside solution, and collecting supernate to obtain the astragaloside-rADSCs; the preparation method comprises the following steps: uniformly mixing PF-127 with sodium hyaluronate, then mixing with astragaloside-rADSCs, stirring, and standing, so as to obtain the astragaloside-rADSCs hydrogel. The invention further provides an application of the prepared astragaloside-rADSCs hydrogel. The astragaloside-rADSCs hydrogel is used for preparing a medicine for repairing diabetic skin injury. According to the astragaloside-rADSCs hydrogel prepared by the invention, the astragaloside is used for regulating the stem cell function and enhancing the repair promotion capability of the stem cell, serum-related risks are avoided through serum-free culture, controlled release of cell factors is realized by virtue of the temperature-sensitive hydrogel, and a brand new non-invasive, non-drug-resistant and efficient repair treatment scheme is provided for diabetic skin injury.

The invention relates to an inducer and an induction method for adipose differentiation of mesenchymal stem cells, and the inducer for adipose differentiation of mesenchymal stem cells is characterized in that 3-isobutyl-1-methylxanthine IBMX, insulin, indometacin, fetal calf serum, dexamethasone sodium phosphate, astragaloside, resveratrol and iridoside A are contained in a DMEM (dulbecco's modified eagle medium) high glucose culture medium. When the mesenchymal stem cells are cultured, only the inducer is used, and different proportions of inducers do not need to be added into a culture medium like the prior art; moreover, the adipose differentiation induction time is shortened, the adipose differentiation induction rate is not lower than 91.3% after culture is carried out for 15 days, and the adipose differentiation induction rate is not lower than 94.8% after culture is carried out for 21 days.

The application discloses a heart-protecting granule identification and quality control method based on a thin layer chromatography, and belongs to the technical field of drug detection. The thin layer chromatography identification method comprises the following steps: taking astragaloside as a control product to identify astragalus membranaceus, taking chrysophanol and emodin as control products to identify largeleaf rhubarb, taking hirudo medicinal materials as a control product to identify hirudo, taking glycyrrhizin and licorice medicinal materials as control products to identify licorice, taking catalpol as a control product to identify radix rehmanniae, taking salvianolic acid B and salvia miltiorrhiza medicinal materials as control products to identify salvia miltiorrhiza, and taking naringin and neohesperidin as control products to identify aurantii fructus. The application establishes the thin layer chromatography identification method of the heart-protecting granule, adopts a special developing agent and a color developing mode, realizes accurate identification according to the spot color, fluorescence characteristics and other characteristics of the corresponding positions of the test product chromatogram, the control product and the control medicinal material chromatogram, the method is simple in operation, low in detection cost, strong in repeatability and specificity, and can effectively control the quality of the heart-protecting granule production process.

The invention relates to a method for extracting astragaloside, which belongs to the field of natural medicines, and comprises the following steps of: crushing astragalus membranaceus, and performing reflux extraction in an alkaline solvent, the pH range of the alkaline environment is 9-13, and the pH range of the alkaline condition is regulated by using alkaline reagents KOH, NaOH and Na2CO3.

The invention relates to a pharmaceutical composition for treating chronic obstructive pulmonary disease and application of the pharmaceutical composition. The pharmaceutical composition is prepared from astragaloside, nobiletin and icariin. The composition disclosed by the invention is rich in raw materials, has the effects of improving airflow limitation, resisting inflammation, resisting oxidation and the like, can be effectively used for treating the chronic obstructive pulmonary disease, and has relatively high economic and social benefits.

The invention discloses a traditional Chinese medicine composition for atherosclerotic diseases, a preparation and a quality control method thereof, and belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition consists of 12 traditional Chinese medicines including 5-25 parts of codonopsis pilosula, 20-50 parts of astragalus membranaceus, 6-25 parts of pseudo-ginseng, 20-50 parts of salvia miltiorrhiza and the like, preferably 10 parts of codonopsis pilosula, 20 parts of astragalus membranaceus, 10 parts of pseudo-ginseng and the like. During preparation, pseudo-ginseng and coptis chinensis are separately crushed, other medicinal materials are decocted twice with water, the decoction is concentrated into extract, and auxiliary materials are added to prepare capsules, tablets and other preparations. According to quality control, panax notoginseng is identified by thin layer chromatography of an optimized developing solvent, and astragaloside content is measured by high performance liquid chromatography. The traditional Chinese medicine composition has the effects of replenishing qi to invigorate the spleen and promoting blood circulation to remove blood stasis, is stable in process and controllable in quality, and is suitable for auxiliary conditioning of qi deficiency and blood stasis type atherosclerotic diseases.

The invention relates to the technical field of biomedical materials, in particular to a chitosan-hyaluronic acid hydrogel composition based on astragaloside and salvianolic acid B as well as a preparation method and application of the chitosan-hyaluronic acid hydrogel composition. According to the hydrogel, a three-dimensional network formed by synergistic crosslinking of chitosan and hyaluronic acid is constructed, various natural active ingredients including astragaloside, salvianolic acid B, ferulic acid and notoginsenoside R1 are loaded, and the composite hydrogel with good biocompatibility, controllable degradability and anti-inflammatory and anti-adhesion functions is formed. C-S covalent crosslinking is realized by adopting a maleimide-cysteine system, so that the structural stability and the component release efficiency are improved. Animal experiment results show that the hydrogel can significantly reduce postoperative adhesion score and inflammatory factor level, and has wide postoperative anti-adhesion application potential.