Conditioned medium preparation of mesenchymal stem cells used for skin aging restoration

A technology of conditioned medium and mesenchymal stem cells, which is applied in the field of induction and culture of biological stem cells, can solve problems such as not being able to meet the needs, and achieve the effect of solving skin aging

Active Publication Date: 2014-09-03
CHENGDU QINGKE BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In the current skin care and beauty industry, traditional chemical beauty and physical beauty can no longer meet the needs. Natural plant extra

Method used

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  • Conditioned medium preparation of mesenchymal stem cells used for skin aging restoration

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Embodiment 1

[0019] Active mesenchymal stem cells were selected, and mesenchymal stem cells were cultured under conventional conditions until their growth density was 60%, and then the medium was changed and washed with PBS solution. The conventional culture condition of the present invention refers to, the medium used is DMEM medium, and its culture temperature is 37 ℃, and the culture system contains 5% carbon dioxide; Wherein, the culture system contains 5% carbon dioxide refers to the carbon dioxide in the stem cell culture environment The concentration is 5%.

[0020] The medium replacement treatment in the present invention means that before using other medium for culture, the mesenchymal stem cells need to be cleaned to avoid mixing the original medium into the new medium. The medium used for conventional treatment is DMEM medium, the medium used for induction is DMEM medium without phenol red, the culture temperature for induction culture is 37° C., and the culture system contains ...

Embodiment 2

[0025] Active mesenchymal stem cells were selected, and the mesenchymal stem cells were cultured under conventional conditions until their growth density reached 80%, and then the medium was changed and washed with PBS solution. The conventional culture condition of the present invention refers to that the medium used is DMEM medium, its culture temperature is 39 ℃, and the culture system contains 6% carbon dioxide; wherein, the culture system contains 6% carbon dioxide refers to the amount of carbon dioxide in the stem cell culture environment The concentration is 6%.

[0026] The medium replacement treatment in the present invention means that before using other medium for culture, the mesenchymal stem cells need to be cleaned to avoid mixing the original medium into the new medium. The medium used for conventional treatment is DMEM medium, the medium used for induction is DMEM medium without phenol red, the culture temperature for induction culture is 37° C., and the cultur...

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Abstract

The invention discloses a conditioned medium preparation of mesenchymal stem cells used for skin aging restoration. A preparing method of the conditioned medium preparation includes: culturing the mesenchymal stem cells under normal conditions until the growth density of the mesenchymal stem cells is 60-80%, performing liquid changing, culturing with a phenol red-free DMEM, adding astragaloside, performing induction culturing in an incubator for 24 h, removing cytokines having a molecular weight lower than 3000 after the culturing is finished, concentrating, detecting the cytokines in the medium, adding 0.01-0.6 mg/mL of reduced glutathione and 0.01-0.4% of hyaluronic acid into the concentrated culture medium solution. The conditioned medium preparation is rich in a plurality of the cytokines. The astragaloside can maintain the characteristics of the stem cell and stimulates the capability of secreting the plurality of the cytokines of the stem cells. The hyaluronic acid avoids the problems of short half-life period of the cytokines and short curative effect time, and enhances the skin restoration effects.

Description

technical field [0001] The invention relates to the technical field of induced culture using biological stem cells, in particular to a conditional culture preparation of mesenchymal stem cells and the application of the preparation in repairing skin aging. Background technique [0002] Skin aging is a very complex process. The main manifestations are the shortening of chromosomal telomeres, the decrease of DNA methylation level, and changes in the expression levels of various cytokines, such as the changes in the number and function of growth factors such as EGF and their receptors. One of the important reasons is subcutaneous vascular atrophy, which leads to Insufficient blood supply to the skin leads to pathophysiological effects. Cells lack nutrients, and their reactivity to the outside world gradually decreases. Skin cell proliferation and differentiation ability weakens, metabolism slows down, and aging symptoms such as wrinkles and spots appear on the skin. [0003] I...

Claims

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Application Information

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IPC IPC(8): A61K35/12A61K38/18A61P17/18A61P17/00A61K38/06A61K31/728A61K31/7048C12N5/0775
Inventor 海泉陈静娴胡忠国赵令卉高雪华王翔王小燕赵峻
Owner CHENGDU QINGKE BIOTECH
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