Preparation method of controlled-release hydrogel with photo-thermal treatment and wound repair functions

A technology for wound repair and photothermal therapy, applied in the field of controlled release hydrogel preparation, can solve the problems of tumor recurrence and inability to clean the tumor, achieve simple experimental operation process, reduce or avoid toxic side effects, and good controlled release performance effect

Active Publication Date: 2019-10-29
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of tumors in our country is still based on surgical resection, but most of the operations cannot completely remove the tumor, which leads to tumor recurrence.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1) Add 1g of alginic acid to 47mL of MES buffer and stir for 2h;

[0038] 2) Dissolve 85 mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 51 mg of N-hydroxysuccinimide in 1 mL of MES buffer respectively;

[0039] 3) Put the solution in step 1) in a water bath and heat up to 60°C, and add the 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloric acid solution in step 2) under nitrogen protection and N-hydroxysuccinimide solution. Stir for 1 h, during which the pH of the solution is maintained at 5 with hydrochloric acid or sodium hydroxide;

[0040] 4) Finally, add 550 mg of dopamine to the solution obtained in step 3), and then react for 12 hours under stirring, and maintain the pH of the solution at 5 with hydrochloric acid or sodium hydroxide during stirring;

[0041] 5) The solution obtained in step 4) was placed in an 8000 Da dialysis bag and dialyzed in deionized water in an acidic environment for 2 days, then frozen in an ultra-low temp...

Embodiment 2

[0050] 1) Add 1.25g of alginic acid to 47.5mL of MES buffer, and stir for 2h;

[0051] 2) Dissolve 87.5 mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 53 mg of N-hydroxysuccinimide in 1.25 mL of MES buffer respectively;

[0052] 3) Put the solution in step 1) in a water bath and heat up to 60°C, and add the 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloric acid solution in step 2) under nitrogen protection and N-hydroxysuccinimide solution. Stir for 1.25h, and maintain the pH of the solution at 5 with hydrochloric acid or sodium hydroxide during stirring;

[0053] 4) Add 570 mg of dopamine to the solution obtained in step 3), and react under stirring for 14 hours. During the stirring, use hydrochloric acid or sodium hydroxide to maintain the pH of the solution at 5;

[0054] 5) The solution obtained in step 4) was placed in an 8000 Da dialysis bag and dialyzed in deionized water in an acidic environment for 2 days, then frozen in an ultra-low ...

Embodiment 3

[0063] 1) Add 1.5g of alginic acid to 48mL of MES buffer, and stir for 2h;

[0064] 2) Dissolve 90mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 55mg of N-hydroxysuccinimide in 1.5mL of MES buffer respectively;

[0065]3) Put the solution in step 1) in a water bath and heat up to 60°C, and add the 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloric acid solution in step 2) under nitrogen protection and N-hydroxysuccinimide solution. Stir for 1.5h, and maintain the pH of the solution at 6 with hydrochloric acid or sodium hydroxide during stirring;

[0066] 4) Add 600 mg of dopamine to the solution obtained in step 3), and then react for 16 hours under stirring, and maintain the pH of the solution at 6 with hydrochloric acid or sodium hydroxide during stirring;

[0067] 5) The solution obtained in step 4) was placed in an 8000 Da dialysis bag and dialyzed in deionized water in an acidic environment for 3 days, then frozen in an ultra-low temperatu...

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Abstract

The invention relates to the field of medical polymers, and discloses a preparation method of a controlled-release hydrogel with photo-thermal treatment and wound repair functions. The method comprises the following steps of: firstly, modifying dopamine on alginic acid to ensure that an o-diphenol side group is arranged on a molecular chain of the alginic acid, and simultaneously preparing coppersulfide @ melanin-PEG @ dopamine nanoparticles with good photo-thermal performance and radiotherapy effect; and then adding the copper sulfide @ dopamine nanoparticles into a dopamine-modified alginicacid solution, adding astragaloside with the function of promoting wound healing into the solution at the same time, and forming gel through a catalytic cross-linking system of horseradish peroxidaseand hydrogen peroxide. The hydrogel has good controlled release performance and can play a role in reducing administration dosage and reducing or avoiding toxic and side effects. The hydrogel prepared by the method has good biocompatibility, can be used for treating cancer cells by utilizing photo-thermal performance and radiotherapy, and can be combined with the astragaloside for promoting postoperative wound healing.

Description

technical field [0001] The invention relates to the field of medical polymer materials, in particular to a method for preparing a controlled-release hydrogel with functions of photothermal therapy and wound repair. Background technique [0002] As a major disease that threatens human life and health, cancer has long been characterized by low cure rate, high recurrence rate and high mortality rate. At present, the treatment of tumors in my country is still dominated by surgical resection, but most operations cannot completely remove the tumor, which leads to tumor recurrence. After surgical resection of the tumor, in order to remove the remaining tumor tissue, we need a treatment method that can act in a small local area and with milder conditions to achieve this goal. Undoubtedly, photothermal therapy that can target specific areas and have mild conditions is a more suitable choice. At the same time, slow postoperative wound healing is also one of the problems that needs to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K47/36A61K47/34A61K41/00A61K31/7048A61K51/12A61K51/06A61P35/00A61P17/02
CPCA61K9/06A61K9/0014A61K47/36A61K47/34A61K41/0052A61K31/7048A61K51/1213A61K51/06A61P35/00A61P17/02A61K2300/00
Inventor 王秉金小康蒋鹏邱方燚万军民
Owner ZHEJIANG SCI-TECH UNIV
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