Preparation method of antimonene-based tumor targeted drug-loaded nanoparticles

A drug-loaded nano- and tumor-targeted technology, applied in the field of medicine, can solve the problems of difficult chemical modification, poor product stability, and few AM surface active groups

Active Publication Date: 2021-04-20
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although antimonene nanomaterials (AM) are a new photothermal therapeutic agent that has emerged recently, its rapid degradation in physiological media greatly limits its direct utilization, and there are few reports on tumor therapy; secondly, AM Few surface active groups, chemical modification is difficult, and the product obtained through van der Waals force and hydrogen bond hydrophobic interaction has poor stability

Method used

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  • Preparation method of antimonene-based tumor targeted drug-loaded nanoparticles
  • Preparation method of antimonene-based tumor targeted drug-loaded nanoparticles
  • Preparation method of antimonene-based tumor targeted drug-loaded nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Chitosan solution preparation: Weigh 20 mg chitosan and dissolve it in 20 ml acetic acid to prepare a 1 mg / mL chitosan solution, and store it in a refrigerator at 4°C.

[0036] (2) Preparation of hyaluronic acid solution: Weigh 20 mg of hyaluronic acid and dissolve it in 20 ml of deionized water to prepare a 1 mg / mL hyaluronic acid solution, and store it in a refrigerator at 4°C.

[0037] (3) Preparation of doxorubicin doxorubicin solution: Weigh 10 mg of ICG and dissolve in 10 ml of deionized water to prepare a 1 mg / mL doxorubicin solution, wrap it in tin foil and store it in a refrigerator at 4°C.

[0038](4) Preparation of AMNPs: Disperse 2g of antimony powder in a clean beaker containing 40ml of NMP at room temperature, seal it with plastic wrap, and use a φ6 ultrasonic probe to sonicate in an ice bath for 8h under an ultrasonic cell disruptor, with a power of 1000w, and stop for 1s every 3s. Stir the dispersion with a glass rod every 30 minutes to make it evenl...

Embodiment 2

[0044] (1) Chitosan solution preparation: Weigh 20 mg chitosan and dissolve it in 10 ml acetic acid to prepare a 2 mg / mL chitosan solution, and store it in a refrigerator at 4°C.

[0045] (2) Preparation of hyaluronic acid solution: Weigh 20 mg of hyaluronic acid and dissolve it in 10 ml of deionized water to prepare a 2 mg / mL hyaluronic acid solution, and store it in a refrigerator at 4°C.

[0046] (3) Preparation of doxorubicin doxorubicin solution: Weigh 20 mg of ICG and dissolve in 10 ml of deionized water to prepare a 2 mg / mL ICG solution, wrap it in tin foil and store it in a refrigerator at 4°C.

[0047] (4) Preparation of AMNPs: Disperse 4 g of antimony powder in a clean beaker containing 80 ml of NMP at room temperature, seal it with plastic wrap, and use a φ6 ultrasonic probe to sonicate in an ice bath for 10 h under an ultrasonic cell disruptor, with a power of 1000 W, and stop for 1 s every 3 s. Stir the dispersion with a glass rod every 30 minutes to make it evenl...

Embodiment 3

[0052] (1) Preparation of chitosan solution: Weigh 15 mg of chitosan and dissolve it in 10 ml of acetic acid to prepare a 1.5 mg / mL chitosan solution, and store it in a refrigerator at 4°C.

[0053] (2) Preparation of hyaluronic acid solution: Weigh 15 mg of hyaluronic acid and dissolve it in 10 ml of deionized water to prepare a 1.5 mg / mL hyaluronic acid solution, and store it in a refrigerator at 4°C.

[0054] (3) Preparation of doxorubicin doxorubicin solution: Weigh 15 mg of ICG and dissolve in 10 ml of deionized water to prepare a 1.5 mg / mL doxorubicin solution, wrap it in tin foil and store it in a refrigerator at 4°C.

[0055] (4) Preparation of AMNPs: Disperse 3g of antimony powder in a clean beaker containing 50ml of NMP at room temperature, seal it with plastic wrap, and use a φ6 ultrasonic probe to sonicate in an ice bath for 8.5h under an ultrasonic cell disruptor, with a power of 1000w, and stop for 1s every 3s , use a glass rod to stir the dispersion every 30 min...

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Abstract

The invention relates to the field of medicines, and discloses a preparation method of antimonene-based tumor targeted drug-loaded nanoparticles. Firstly, antimonene nanoparticles are obtained through a liquid phase mechanical stripping method and differential centrifugation, then the surface of AMNPs with negative charges is coated with chitosan and adriamycin with positive charges through the electrostatic adsorption effect, and finally the surface of chitosan is coated with hyaluronic acid with negative charges; and the photo-thermal/chemotherapy drug-loaded nanoparticles capable of realizing tumor targeting, drug sustained release and multi-modal imaging are obtained. The drug-loaded nanoparticles have important development prospects in the aspects of anti-tumor photo-thermal photodynamic therapy, drug nano-carriers and the like.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a preparation method of antimonene-based tumor-targeting drug-loaded nanoparticles. Background technique [0002] Traditional tumor treatment methods have high recurrence rate and large side effects, cannot achieve precise treatment of malignant tumors, and have certain damage to normal tissues. With the rise of photothermal photodynamic therapy as a new tumor treatment modality, finding suitable materials has become our top priority. Since antimonene was first reported in 2015, due to its excellent physicochemical properties, it has gradually attracted widespread attention, including its application prospects in cancer therapy. Although antimonene nanomaterials (AM) are a new photothermal therapeutic agent that has emerged recently, its rapid degradation in physiological media greatly limits its direct utilization, and there are few reports on tumor therapy; secondly, AM There are fe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K31/704A61K47/36A61K41/00A61K47/61A61K47/69A61K49/00A61K49/22A61P35/00B82Y5/00B82Y20/00B82Y30/00B82Y40/00
Inventor 王秉泮林丹金小康陈浩彭志勤万军民
Owner ZHEJIANG SCI-TECH UNIV
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