279 results about "Tumor recurrence" patented technology

Provided are compositions and methods of use for insect cells comprising baculovirus encoding non-surface expressed proteins and peptides. The claimed invention particularly relates to compositions comprising insect cells containing baculovirus that express cytokines. Such compositions may be administered by, for example, direct intratumoral injection into tumors in mammals, resulting in tumor reduction or recission. Another aspect of the claimed invention concerns methods of promoting resistance to the reoccurence of tumors in mammals who have undergone such tumor recission. In a specific aspect of the claimed invention, the mammals are human subjects presenting with various forms of cancer.

The invention relates generally to the field of cancer prognosis and treatment. More particularly, the present invention relates to methods and compositions that utilize a particular panel of gene products (“biomarkers”) and their differential expression patterns (“expression signatures”), wherein the expression patterns correlate with responsiveness, or lack thereof, to chemotherapy treatment. The invention is based on the identification of a specific set of biomarkers that are differentially expressed in chemotherapy-treated tumors and which are useful in predicting the likelihood of a therapeutic response, including residual disease persistence and subsequent tumor recurrence in cancer patients receiving chemotherapy. The gene panel is also useful in designing specific adjuvant modalities with improved therapeutic efficiency. Also disclosed are methods for characterizing tumors according to expression of the biomarkers described herein.

The present inventors have shown that the gene and protein expression profiles of ADAM8, ADAM10 and ADAM12 in different grades and stages of bladder cancer.ADAM12 gene expression was evaluated in tumors from 96 patients with bladder cancer using a customized Affymetrix GeneChip. Gene expression in bladder cancer was validated using reverse transcription-polymerase chain reaction (RT-PCR), quantitative PCR, and in situ hybridization. Protein expression was evaluated by immunohistochemical staining on tissue arrays of bladder cancers.The presence and relative amount of ADAM12 in the urine of cancer patients were determined by Western blotting and densitometric measurements, respectively.Particularly ADAM12 mRNA expression was significantly upregulated in bladder cancer, as determined by microarray analysis, and the level of ADAM12 mRNA correlated with disease stage. ADAM12 protein expression correlated with tumor stage and grade. ADAM12 was present in higher levels in the urine from bladder cancer patients than in urine from healthy individuals. Significantly, following removal of tumor by surgery, in most bladder cancer cases examined the level of ADAM12 in the urine decreased and, upon recurrence of tumor, increased.

A method is provided for assessing allelic losses on specific chromosomal regions in melanoma patents. The method relies on the evidence that free DNA may be released in the plasma / serum of cancer patients allowing the detection of DNA with LOH in the plasma / serum of cancer patients by analysis for microsatellite markers. The amount of and specific allelic loss allows a prognosis to be made regarding tumor diagnosis and progression, tumor metastasis, tumor recurrence, and mortality.

The invention relates to subject-specific methods for detecting recurrence of tumours based on an understanding of the clonal / subclonal mutation profile of the subject's tumour and detection of the mutations in their cell-free DNA (cfDNA), typically by multiplex PCR of tumour mutations such as single nucleotide variants (SNVs).

A method for reducing the recurrence of a resectable malignant tumor includes administering an immunostimulatory dosage of an α-interferon composition, then surgically resecting the malignant tumor. A method for treating a human patient having a non-resectable malignant tumor includes administering an immunostimulatory dosage of an α-interferon composition to the patient and treating the patient with effective non-surgical medical methodologies to diminish the tumor. An article of manufacture combines an α-interferon composition within a packaging material and a package label or insert indicating that administration of an immunostimulatory dosage of an α-interferon composition followed by surgical resection of a malignant tumor can be effective for treating a human patient having the malignant tumor.

The invention relates to methods for treating of tumor metastasis, methods for preventing, inhibiting, and predicting tumor recurrence and tumor metastasis, and methods of preventing cancer development for one at risk of developing cancer, for one diagnosed with a benign cancer, and / or for one diagnosed with malignant cancer. The invention also relates to methods of treating angiogenesis-dependent diseases and disorders. In addition, the invention provides: (1) a method for screening for tumor / cancer derived angiogenesis and metastasis factors; (2) a method for screening for compounds that inhibit angiogenesis and metastasis; (3) a method for screening for compounds that promote anti-angiogenesis and anti-metastasis activities; (4) a method for cancer prognosis evaluation; (5) a method for predicting the tissue specificity of a metastatic cancer; (6) a method for determining likelihood of metastasis; and (7) a method for cancer prognosis evaluation by the surveillance of metastasis development.

This invention pertains to a method of low-cost intraoperative all field simultaneous parallel microbeam single fraction few seconds duration 100 to 1,000 Gy and higher dose radiosurgery with micro-electro-mechanical systems (MEMS)-carbon nanotube based microaccelerators. It ablates cancer cells including the mesenchymal epithelial transformation associated cancer stem cells. Microbeam brachy-therapeutic radiosurgery is performed. Microaccelerators are configured for simultaneous parallel microbeam emission from varying angels to an isocentric tumor. Their additive dose rate at the isocentric tumor is in the range of 10,000 to 20,000 Gy / s. It eliminates most tumor recurrence and metastasis which enhances cancer cure rates. It also exposes cancer antigens which induces cancer immunity. Stereotactic breast core biopsy is combined with, positron emission tomography and computerized tomography and phase-contrast imaging. Parallel microbeam brachytherapy preserves normal breast appearance. Migration of normal stem cells from unirradiated valley regions heals the radiation damage to the normal tissue.

The invention provides methods of estimating the likelihood of lung cancer recurrence in a subject, including those afflicted with NSCLC. The methods of the invention are useful for developing a therapeutic treatment plan to prevent cancer recurrence for subjects deemed to be at high risk, and withholding treatments from those subjects deemed to be at low risk. The invention also provides methods of generating and using metagene-based prediction tree models for estimating the likelihood of lung cancer recurrence. The invention also provides reagents, such as DNA microarrays, software and computer systems useful for estimating cancer recurrence, and provides methods of conducting a diagnostic business for the prediction of cancer recurrence.

The invention provides a method to reduce the rate of tumor metastasis and tumor recurrence after surgery and chemotherapy and radiotherapy by removing circulating tumor cells (CTC) in the blood after surgery and chemotherapy and radiotherapy by means of specificity, thereby improving the survival rate of cancer patients. The invention provides a method and a system for removing tumor cells. Patients ' blood is guided out to pass through a container, an antibody or a ligand capable of combining with the specificity of the tumor cells is fixed on a solid-phase carrier in the container, whne the patients ' blood passes through the container, the tumor cells can be absorbed by the specificity to be unable to flow back to patients ' blood, and then the tumor cells are removed from the blood to reduce the recidivation of tumor patients and to prolong their survival rate. The removed tumor cells can be washed out from the solid-phase carrier for counting. The invention further provides a method to remove pathogen from blood. Blood is withdrawn from a patient and its plasma and cellular components are separated by means of a plasma separator under the effect of a blood pump, the plasma portion inactivates the pathogen in the blood via a pathogen inactivating device, flows back to extracorporeal circulation passage to merge with the cellular components and then is returned to the patient. The method adopts a physical means, so that side effects can be reduced. The device is simple in structure and can be connected with other blood purification devices.

The invention belongs to the technical field of biology, and discloses a polypeptide containing two chimeric antigen receptor structures, nucleic acid for coding the polypeptide, a modified T lymphocyte, and a preparation method and application of the T lymphocyte. The polypeptide provided by the invention contains the two chimeric antigen receptor (CAR) structures, wherein one chimeric antigen receptor structure specifically targets a tumor cell, and the other one specifically targets a normal B lymphocyte, so that a CAR-T cell is effectively amplified through recognizing the B lymphocyte ofa blood circulation system, and more CAR-T cells arrive at a solid tumor part so as to specifically kill the tumor cell for improving a treatment effect on the solid tumor. The polypeptide further comprises an ScFv structure of an anti-PD-L1 monoclonal antibody, and the CAR-T cells can simultaneously secrete a PD-L1 monoclonal antibody which interdicts an immunosuppressive action medicated by a PD1 / PDL1 signal pathway and T cell failure, so that the treatment effect on the solid tumor is improved, the functional activity of immune cells in vivo is prolonged at the same time, and finally the tumor recurrence is retarded and even avoided.

The invention provides survivability-screened cancer stem cells, a preparation method thereof, the application of the survivability-screened cancer stem cells in preparing antineoplastic agents, a kit for preparing the survivability-screened cancer stem cells, an antigen composite which is obtained from the survivability-screened cancer stem cells, a preparation method thereof, the application of the antigen composite in preparing antineoplastic agents and a kit for preparing the antigen composite. The antineoplastic agents which are prepared by the survivability-screened cancer stem cells and the antigen composite which is obtained from the survivability-screened cancer stem cells can stimulate inner body immune response and outer body immune response to kill the cancer stem cells which cause tumor recurrence so as to provide a probability for conquering the survivability of the tumor and radically curing the recurrence and the metastasis of the tumor.

The present disclosure provides compositions and methods for the delivery of immune cells to treat un-resectable or non-resected tumor cells and tumor relapse. The compositions comprise (i) a structure comprising an injectable polymer or scaffold comprising pores; (ii) lymphocytes disposed within the structure, (iii) at least one lymphocyte-adhesion moiety associated with the structure; and (iv) at least one lymphocyte-activating moiety associated with the structure, and optionally an immune stimulant.

The invention relates to the compounds and methods for inhibiting the Cyclin-Dependent Kinase Inhibitor (CDKI) pathway. More particularly, the invention relates to compounds and methods for inhibiting the CDKI pathway for studies of and intervention in senescence-related and other CDKI-related diseases. The invention provides new compounds having improved solubility and / or potency, and methods for their use. In various aspects, the invention relates to the treatment of cancer. The invention provides methods for chemoprevention and prevention of tumor recurrence or metastasis. The invention further provides diagnostic techniques for treatment for certain cancer types. The invention utilizes specific inhibitors of CDK8 / 19 and / or measurement of CDK8 levels in a patient.

The invention an article or composition of mammalian extracellular matrix (ECM) for placement in a lumpectomy space of a breast after tumor removal. The extracellular matrix article or composition can regenerate lost breast tissue, reduce the formation of scar tissue at the site of excision, and reduce a likelihood of local tumor recurrence at the lumpectomy site. The article can be sheet ECM, and the composition can be particulate ECM or emulsion or gel ECM.

The present invention relates to the use of immunogenic peptides comprising a T-cell epitope derived from a tumour-associated antigen and a redox motif such as C—(X)2-[CST] or [CST]-(X)2-C in the treatment of a tumour or in the treatment or prevention of a tumour relapse, and in the manufacture of medicaments therefore.

The invention discloses human T lymphocytes carrying a CD20 / CD19 bispecific chimeric antigen receptor as well as a preparation method and application of the human T lymphocytes. The human T lymphocytes are activated by means of an antibody, and the CD20 / CD19 bispecific chimeric antigen receptor is obtained by means of tandem of an anti-human CD20 antibody and an anti-human CD19 antibody. The preparation method provided by the invention can significantly increase the proportion of CD3+CD8+T cells in the T lymphocytes, obviously increases the virus transduction efficiency, can improve the coverage rate of CART cells on tumors, and prevents tumor recurrence after CD19 or CD20 single-target-point CART treatment.

The present invention relates to the use of immunogenic peptides comprising a T-cell epitope derived from a tumour-associated antigen and a redox motif such as C-(X)2-[CST] or [CST]-(X)2-C in the treatment of a tumour or in the treatment or prevention of a tumour relapse, and in the manufacture of medicaments therefore.

The invention relates to a method for performing prognosis condition grouping on a hepatocellular carcinoma patient who has single tumor and is about to be subjected to or is already subjected to orthotopic liver transplantation. The method comprises the steps of (1) obtaining a sample from general samples after transhepatic biopsy before transplantation or liver transplantation, and detecting CK19 and GPC3 expression conditions of tumor cells in the hepatocellular carcinoma tissue sample of the patient; (2) detecting and recording a maximum diameter of the tumor of the patient; (3) detecting and recording a serum alpha-fetoprotein level of the patient; and (4) based on a pathological section, observing whether microvascular invasion exists or not. Recurrent risks are scored according to beta coefficients of four factors in COX regression analysis; scores in items are subjected to addition; a recurrent risk group with a total score less than or equal to 4 is determined as a low recurrent risk group; a recurrent risk group with a total score equal to 5-7 is determined as a medium recurrent risk group; and a current risk group with a total score more than or equal to 8 is determined as a high recurrent risk group. Through the method, an effective auxiliary information value can be provided for a doctor and the patient; and tumor recurrence or transfer is closely monitored, so that health conditions can be discovered within the shortest time, the doctor and the patient are effectively prompted, and people are reminded to timely examine body conditions.

The present invention provides methods of immunizing a subject against a tumor, inhibiting tumor growth, inhibiting tumor recurrence, treating, suppressing the growth of, or decreasing the incidence of a tumor, overcoming tolerance to a tumor vasculature marker (TVM) in a subject comprising the step of administering a vaccine comprising a TVM or a nucleic acid encoding a TVM and related vaccines. The present invention also provides a method of targeting a tumor vasculature in a subject having a tumor comprising the step of contacting said subject with a labeled compound that binds a) a tumor vasculature marker (TVM) or b) a nucleic acid molecule encoding said TVM.

The present invention relates to methods for inhibiting tumor growth, increasing survival of a subject having a tumor and inducing protection against tumor recurrence in a mammal. The methods comprise administering a humanized monoclonal antibody comprising CDR regions derived from the murine monoclonal antibody designated mBAT-1, in combination with at least one chemotherapeutic agent.

A Chinese medicine for preventing and treating cancers is prepared from the fermented cordyceps powder, ganoderma and wolfberry fruit.

The invention relates to a liquid biopsy detection method, particularly a liquid biopsy detection method for circulating tumor cells. The method includes enriching circulating tumor cells in a sample;separating and identifying the circulating tumor cells; and quantitatively counting the circulating tumor cells in unit volume. The intelligent liquid biopsy method combines the newest computer science and biological techniques, and innovatively provides a circulating tumor cell quantitative detection method, a circulating tumor cell separating method and a circulating tumor cell in-vitro culturemethod. The liquid biopsy detection method can achieve dynamic monitoring of tumor traits, tumor treatment and prognosis evaluation, tumor recurrence monitoring, and the like, and therefore the liquid biopsy detection method will greatly promote developments of individualized precise treatment in China.

The invention relates to compositions, kits, and methods for alleviating cancer (i.e., a tumor) in a human patient. The therapeutic modality effected by the invention involves inducing a type 1 inflammatory response in the tumor tissue, whereby the tumor tissue is diminished or destroyed and the patient develops immune memory that inhibits or prevents recurrence of the tumor.

The invention provides a dendritic cell carried by a tumor stem cell antigen and subjected to tolerance screening and a preparation method thereof, application of the dendritic cell carried by the tumor stem cell antigen and subjected to the tolerance screening in preparing anti-tumor medicament, a kit for preparing the dendritic cell carried by the tumor stem cell antigen and subjected to the tolerance screening, and a vaccine comprising the dendritic cell carried by the tumor stem cell antigen and subjected to the tolerance screening. The dendritic cell carried by the tumor stem cell antigen and subjected to the tolerance screening and the medicament prepared by an immune composition of the dendritic cell carried by the tumor stem cell antigen and subjected to the tolerance screening can kill tumor stem cells causing tumor relapse in vivo and in vitro so as to provide possibility for overcoming the tumor tolerance and radically treating the relapse and transfer of tumor.

The invention discloses a human body tumor recurrence monitoring and thermal therapy integrated system which comprises a scanning bed, a monitoring body frame and a computer. The scanning bed is arranged on one side of the monitoring body frame, the monitoring body frame is provided with an optical fiber probe, an infrared temperature measuring probe, a laser thermal therapy device and a microwave thermal therapy device, a monitoring and thermal therapy main control system is arranged in the computer, the optical fiber probe and the infrared temperature measuring probe are respectively connected with the signal output end of the monitoring and thermal therapy main control system, and the laser thermal therapy device and the microwave thermal therapy device are respectively connected with the signal output end of the monitoring and thermal therapy main control system. According to the human body tumor recurrence monitoring and thermal therapy integrated system, a therapy system and a diagnosis system are combined together, manual analysis time is not needed, exacerbation of a patient caused by analysis errors is avoided, and health recovery of the patient is guaranteed.

The invention discloses a chimeric antigen receptor targeting human GPC3, the chimeric antigen receptor comprises an extracellular antigen binding region, a transmembrane region and an intracellular signal structural domain which are sequentially connected in series, the extracellular antigen binding region is combined with a GPC3 antigen, the extracellular antigen binding region comprises anti-GPC3 scFv used for combining the GPC3 antigen, wherein a heavy chain amino acid sequence is shown as SEQ ID NO. 1, and a light chain amino acid sequence is shown as SEQ ID NO. 2. Compared with the priorart, the chimeric antigen receptor is constructed by taking the human GPC3 as a target spot, and particularly, amino acids in ITAM2 and ITAM3 regions of CD3zeta are mutated, so that constructed T cells modified by the chimeric antigen receptor have stronger killing activity on tumor cells; moreover, more central memory T cells are provided, GPC3 positive tumor cells are killed in a targeted manner, the tumor recurrence rate after treatment is effectively reduced, and the life cycle of a patient is prolonged.

The invention provides a double-specificity anti-tumor recombinant adenovirus. The recombinant adenovirus comprises an adenovirus vector and expression boxes introduced therein; the replication of the adenovirus vector depends on gene mutation; and the expression boxes comprise an expression box consisting of tumor specific promoters and E1a genes and an expression box consisting of eukaryotic promoters and Apoptin genes. The invention also provides a construction mode of the double-specificity anti-tumor recombinant adenovirus and tumor treatment medicament application thereof. The recombinant adenovirus of the invention has double specificities of tumor specific replication and tumor specific killing, can be used for preparing medicaments for treating various tumors and medicaments for preventing tumor recurrence after operation, and meanwhile can be used with any medicament or used as a medicament combined with surgical therapy, radiotherapy and chemotherapy.

The invention discloses a fiber film coated with gold nanorods, and a preparation method and an application thereof. The fiber film coated with the gold nanorods is composed of PEG surface modified gold nanorods and a fiber film; the gold nanorods are evenly distributed in the fiber film. Based on the PEG surface modified gold nanorods, local high temperature of a tumor part is caused by extremely-high photo-thermal conversion efficiency, tumor cells can be effectively killed, and moreover, with utilization of high biocompatibility of the biodegradable electrospinning fiber film, the electrospinning fiber film is used as a carrier of the gold nanorods and a postoperation recovery material. The electrospinning fiber film can directly act on affected parts, and has a characteristic of regulating and controlling release according to the component ratio to achieve the purpose of slow-release treatment, so as to prevent tumor recurrence after tumor operation.