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Dendritic cell carried by tumor stem cell antigen and subjected to tolerance screening, preparation method and application thereof, kit and vaccine comprising dendritic cell

A technology of tumor stem cells and dendritic cells, applied in the field of dendritic cells and their preparation, can solve the problems of recurrence and metastasis, tumor drug resistance, etc.

Inactive Publication Date: 2009-10-21
北京弘润天源基因生物技术有限公司
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  • Abstract
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Problems solved by technology

[0005] In order to overcome the problems of drug resistance, recurrence and metastasis of tumors after radiotherapy and chemotherapy in existing cancer immunotherapy methods targeting common tumor stem cells, the purpose of the present invention is to provide a tumor stem cell antigen-loaded drug that has been screened for resistance Dendritic cells and preparation method thereof, application of the resistance-screened tumor stem cell antigen-loaded dendritic cells in the preparation of antitumor drugs, preparation of the tolerance-screened tumor stem cell antigen-loaded dendritic cells A kit for dendritic cells, and a vaccine comprising the resistance-screened tumor stem cell antigen-loaded dendritic cells

Method used

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  • Dendritic cell carried by tumor stem cell antigen and subjected to tolerance screening, preparation method and application thereof, kit and vaccine comprising dendritic cell
  • Dendritic cell carried by tumor stem cell antigen and subjected to tolerance screening, preparation method and application thereof, kit and vaccine comprising dendritic cell
  • Dendritic cell carried by tumor stem cell antigen and subjected to tolerance screening, preparation method and application thereof, kit and vaccine comprising dendritic cell

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preparation example Construction

[0035] The present invention provides a method for preparing tumor stem cells screened by tolerance, wherein the method includes:

[0036] a step of irradiating and / or screening for chemotherapeutic drugs on tumor stem cells,

[0037] The radiation irradiation screening is accomplished by exposing the tumor stem cells to a radiation source of radiation selected from the group consisting of X-rays, beta-rays, alpha-rays and gamma-rays,

[0038] The chemotherapeutic drug screening is completed by contacting tumor stem cells with chemotherapeutic drugs, and the chemotherapeutic drugs are selected from alkylating agent chemotherapeutic drugs, nitrosourea chemotherapeutic drugs, antimetabolite chemotherapeutic drugs, antibiotic chemotherapeutic drugs, plant chemotherapeutic drugs, hormones and endocrine drugs Chemotherapy drugs, antibody blocker chemotherapy drugs, phytochemotherapy drugs, antibody blocker chemotherapy drugs, asparaginase, procarbazine, dacarbazine, cisplatin, carb...

preparation example 1

[0120] Preparation Example 1: Isolation and Culture of Tumor Stem Cells in Brain Glioma Tissue

[0121] The glioma tissue was obtained from a 50-year-old male patient who was diagnosed with astroglioma by pathological examination. An informed consent was signed with the patient, and his tumor tissue was collected during the operation. Use ophthalmic scissors to cut the glioma tissue into about 1mm 3 of small pieces. The resulting chopped tissue pieces were digested with 0.25% trypsin solution at a weight-to-volume ratio of 1 g: 0.5 mL for 5 minutes, and then calf serum (U.S. Hyclone Company), which was one-fifth of the volume of the trypsin solution, was added to stop. Centrifuge at 1000rpm to collect the digested tissue fragments, pour off the supernatant of the trypsin solution, and then use HBSS containing 0.1% dispase (dispase, American Sigma) (1IU / mg) and 0.01% DNAse (DNase, Shanghai Shenggong) (Hans buffer, Shanghai Sangong) was washed three times with a weight-to-vol...

preparation example 2-3

[0127] The tumor stem cells of colon cancer, prostate cancer, and brain glioma were respectively prepared according to the method of Preparation Example 1. Among them, the differences are as follows:

[0128] Colon cancer tissue was obtained from a 40-year-old male patient who was diagnosed with moderately differentiated colon adenocarcinoma by pathological examination. An informed consent was signed with the patient, and his tumor tissue was collected during the operation.

[0129] The breast cancer tissue came from a 39-year-old female patient who was diagnosed with breast cancer by pathological examination. An informed consent was signed with the patient, and his tumor tissue was collected during the operation.

[0130] Table 2

[0131]

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Abstract

The invention provides a dendritic cell carried by a tumor stem cell antigen and subjected to tolerance screening and a preparation method thereof, application of the dendritic cell carried by the tumor stem cell antigen and subjected to the tolerance screening in preparing anti-tumor medicament, a kit for preparing the dendritic cell carried by the tumor stem cell antigen and subjected to the tolerance screening, and a vaccine comprising the dendritic cell carried by the tumor stem cell antigen and subjected to the tolerance screening. The dendritic cell carried by the tumor stem cell antigen and subjected to the tolerance screening and the medicament prepared by an immune composition of the dendritic cell carried by the tumor stem cell antigen and subjected to the tolerance screening can kill tumor stem cells causing tumor relapse in vivo and in vitro so as to provide possibility for overcoming the tumor tolerance and radically treating the relapse and transfer of tumor.

Description

technical field [0001] The invention relates to a dendritic cell and a preparation method thereof, the application of the dendritic cell in preparing antitumor drugs, a kit for preparing the dendritic cell, and a vaccine comprising the dendritic cell. In particular, the present invention relates to tolerance-screened tumor stem cell antigen-loaded dendritic cells and a preparation method thereof, and the application of the tolerance-screened tumor stem cell antigen-loaded dendritic cells in the preparation of antitumor drugs , a kit for preparing the resistance-screened tumor stem cell antigen-loaded dendritic cells, and a vaccine comprising the tolerance-screened tumor stem cell antigen-loaded dendritic cells. Background technique [0002] Current tumor treatments include surgery, radiation therapy, and chemotherapy. However, the existing tumor treatment methods cannot cure the tumor. The main reason is that the cells that make up the tumor contain tumor stem cells, which...

Claims

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Application Information

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IPC IPC(8): C12N5/08C12N13/00C12Q1/02A61K39/00A61P35/00
Inventor 沈丽郝丽敏王亚军马艳玲黄启明
Owner 北京弘润天源基因生物技术有限公司
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