15 results about "Sapovirus" patented technology

The present invention addresses the problem of providing: a genetically modified cultured cell or a genetically modified animal each allowing a human sapovirus to proliferate regardless of host characteristics as barriers to proliferation in mammalian cells; and an application of the cell or animal to a screening method. The present inventors have solved the problem by finding that a genetically modified cultured mammalian cell having a human CD36-encoding gene incorporated therein and a mammal having such a cell as a self-cell show acquired susceptibility to human sapovirus infection, and providing, for example, a screening method for a drug relevant to a human sapovirus on the basis of the finding.

The present disclosure is directed to novel porcine sapoviruses (SaV) isolates, including SaV serially propagated in cell culture, all of which are useful in the preparation of immunogenic compositions and vaccines for treating and preventing disease in swine.

The present invention relates to the technical field of virus detection, and discloses a sewage diarrhea virus typing detection system, which comprises a pathogen enrichment unit, an improved polyethylene glycol precipitation method is adopted to carry out pathogen enrichment on a sewage sample; and the nucleic acid extraction unit is used for extracting nucleic acid in the enriched sample by using a magnetic bead extraction method. According to the typing detection system for the diarrhea viruses in the sewage, simultaneous detection and typing of various diarrhea viruses such as norovirus, rotavirus, adenovirus, astrovirus, parareovirus and fiveleaf virus in the sewage can be realized, the detection range is widened, powerful support is provided for comprehensive monitoring of virus pollution conditions in the sewage, and the system is suitable for popularization and application. According to the present invention, the multiple fluorescent RT-PCR technology is adopted, and the efficient sample enrichment and nucleic acid extraction method is combined, such that the detection sensitivity is improved, the low-concentration viral nucleic acid can be detected, and the potential viral threat is not missed;

The present disclosure relates to novel swine Sarbovirus (SaV) isolates, including SaVs continuously propagating in cell culture, all of which are useful in the preparation of immunogenic compositions and vaccines for the treatment and prevention of diseases in swine.

The invention belongs to the technical field of biological detection, and particularly relates to a primer probe combination, a kit and a method for detecting eight porcine viruses. Specific primers and probes are designed for porcine circovirus type 2, porcine circovirus type 3, African swine fever virus, rotavirus, porcine hepatitis E virus, classical swine fever virus, norovirus and fiveleaf virus, and the kit is used for detecting eight viruses of pigs and has the advantages of high specificity, high sensitivity, stable effect and simplicity and convenience in operation; according to the method, eight infection pathogens can be detected, and the method is of great significance to the domestic pig feeding industry and wild boar epidemic disease monitoring and controlling work.

The present invention addresses the problem of providing a cultured cell line that has a constant susceptibility to infection by human sapovirus as well as providing measures for establishing said cultured cell line, and of contributing to research on human sapovirus infections and to the development of therapeutic and preventive measures; the inventors of the present invention achieved the present invention after finding that, by performing transformation using a Cas9 gene on Caco-2, which is an easily obtainable commercial cell line derived from human colon cancer, it is possible to acquire, as clones, cells having constant susceptibility to infection by human sapovirus, that a dramatically improved propagation efficiency of human sapovirus is observed in this human sapovirus infection-susceptible cell line, and that propagation by using a subculture is also possible.

The present disclosure is directed to novel porcine sapoviruses (SaV) isolates, including SaV serially propagated in cell culture, all of which are useful in the preparation of immunogenic compositions and vaccines for treating and preventing disease in swine. The disclosure further provides characterization of pathogenicity and cross-protective immunity of contemporary porcine SaV isolates in weaned pigs, demonstrating that prior exposure to homologous strains provides greater protection against viral shedding than heterologous strains.

The invention discloses a fiveleaf virus whole genome capturing method, a primer group and a kit. Eight groups of specific primer pools are designed for the genome of the fiveleaf viruses, the GI type, the GII type, the GIV type and the GV type of the fiveleaf viruses can be effectively amplified, amplification products have high specificity and fidelity, and a new direction is provided for effective detection of the fiveleaf viruses.

The application provides a neutralizing antibody against a sapovirus and a preparation method and application thereof. A heavy chain CDR1 of the neutralizing antibody comprises an amino acid fragment as shown in SEQ ID NO. 1, a heavy chain CDR2 comprises an amino acid fragment as shown in SEQ ID NO. 2, and a heavy chain CDR3 comprises an amino acid fragment as shown in SEQ ID NO. 3; a light chain CDR1 of the neutralizing antibody comprises an amino acid fragment as shown in SEQ ID NO. 4, a light chain CDR2 comprises an amino acid fragment as shown in SEQ ID NO. 5, and a light chain CDR3 comprises an amino acid fragment as shown in SEQ ID NO. 6. The neutralizing antibody can specifically bind to a conserved epitope on the RBD domain on the spike protein of the sapovirus, thereby preventing the infection of the sapovirus on cells in a broad spectrum, and finally achieving a protection effect.

The present invention addresses the problem of providing: a genetically modified cultured cell or a genetically modified animal each allowing a human sapovirus to proliferate regardless of host characteristics as barriers to proliferation in mammalian cells; and an application of the cell or animal to a screening method. The present inventors have solved the problem by finding that a genetically modified cultured mammalian cell having a human CD36-encoding gene incorporated therein and a mammal having such a cell as a self-cell show acquired susceptibility to human sapovirus infection, and providing, for example, a screening method for a drug relevant to a human sapovirus on the basis of the finding.

The present disclosure provides antibodies and antigen-binding fragments thereof that can bind to the S protein of Sabebe viruses (in some embodiments, including a plurality of Sabebe viruses) and, in certain embodiments, are capable of neutralizing infection by a plurality of Sabebe viruses.

The invention discloses a nano antibody TnbE12 capable of neutralizing a sand shell virus in a broad spectrum and application of the nano antibody TnbE12. The invention provides a TnbE12 nano antibody. Three CDRs in a variable region of the TnbE12 nano antibody are sequentially shown as the 26 site to the 33 site, the 51 site to the 57 site and the 96 site to the 105 site in SEQ ID NO: 9. The invention also provides a TnbE12 humanized nano antibody, which comprises a TnbE12 nano antibody variable region and a human Fc region. The TnbE12 nano antibody and the TnbE12 humanized nano antibody can be used for inhibiting and / or neutralizing coronavirus, so that diseases caused by the coronavirus are prevented and / or treated. The invention has important application value for prevention and control of coronavirus.

The invention belongs to the field of biological medicine, and particularly relates to a novel medical application of a dopamine receptor stimulant pramipexole (PPX) as an inhibitor of the activity of sapovirus 2C RNA helicase ATPase, and the inhibitor is pramipexole. In-vitro enzymology experiments prove that the pramipexole can remarkably inhibit the ATP enzyme activity of the SaV 2C RNA helicase, and the inhibition effect of the pramipexole is quantitatively verified through a malachite green-ammonium molybdate colorimetric method. The invention further discloses that pramipexole and SaV-2C RNA helicase form stable interaction (the binding energy is-126.53 kJ / mol) through molecular docking, and the key interaction comprises four hydrogen bonds mediated by Asp47 and Glu48 residues, hydrophobic interaction between the four hydrogen bonds and Leu30, Ala31 and Trp46, and electrostatic force generated by the four hydrogen bonds and Glu48. The invention has the potential of preventing and treating sapovirus infection diseases.

Nutraceutical compositions and methods of their use in treating Caliciviridae family viruses are provided. The viruses can be norovirus or sapovirus. An exemplary nutraceutical composition includes Fagopyrum dibotrys extract. Another nutraceutical composition for treating viral gastroenteritis includes 5, 7, 3′, 4′-tetrahydroxyflavan-3-ol C4-C8 dimers, quercetin, rutin, or a combination thereof. The nutraceutical compositions inhibit or reduce entry of Caliciviridae family viruses into intestinal epithelial cells of the subject.

The present invention describes immunogenic compositions containing immunogenic polypeptides of Sapovirus, including immunogenic compositions containing antigens other than Sapovirus antigens, including antigens that may be used in immunization against pathogens that cause diarrheal diseases. Methods of eliciting an immune response with the immunogenic compositions as disclosed and methods of treating a Sapovirus infection are also described.