Nucleic acid composition and kit for detecting diarrhea viruses as well as use method of kit

A nucleic acid composition and a technology for diarrhea virus, applied in the biological field, can solve the problems of low sensitivity, poor specificity, and time-consuming virus isolation

Inactive Publication Date: 2019-04-09
深圳市刚竹医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cell culture method takes a long time to isolate viruses and the operation is complicated; PCR and immunological methods can only detect one or two pathogens, which cannot fully meet the needs of detecting various types of diarrhea viruses
In recent years, a double or triple fluorescent quantitative PCR method using Taqman probe method to detect rotavirus, norovirus, astrovirus, sacrovirus, enteric adenovirus, etc. has also appeared. In this method, various primers of diarrhea virus are mixed During simultaneous detection, multiple primers of diarrhea virus are likely to interfere with each other, so the specificity is poor and the sensitivity is not high

Method used

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  • Nucleic acid composition and kit for detecting diarrhea viruses as well as use method of kit
  • Nucleic acid composition and kit for detecting diarrhea viruses as well as use method of kit
  • Nucleic acid composition and kit for detecting diarrhea viruses as well as use method of kit

Examples

Experimental program
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Effect test

Embodiment 1

[0042](1) Using the RNA extraction kit (Tiangen Bacterial RNA Extraction Kit), RNA was extracted from 9 positive samples containing different viruses respectively, and 9 positive samples to be tested containing only RNA of one virus were obtained. The 9 positive samples corresponded to those containing only group A rotavirus, group B rotavirus, group C rotavirus, norovirus, norovirus type I, norovirus type II, saruvirus, and astrovirus , or a positive sample of enteric adenovirus. The specific steps for obtaining each positive sample to be tested are carried out in accordance with the product instructions.

[0043] (2) Mix the 9 kinds of positive test samples obtained in step (1) to obtain the test samples. Three groups of samples to be tested are respectively mixed with the nucleic acid composition and the PCR reaction buffer to obtain three groups of reaction solutions. Wherein, the PCR reaction buffer is a mixture of 10×HSbuffer, hot start HS Taq enzyme, MMLV reverse tran...

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Abstract

The invention relates to nucleic acid composition and a kit for detecting diarrhea viruses as well as a use method of the kit. The nucleic acid composition for detecting diarrhea viruses comprises anamplification primer pair and detection probes corresponding to amplification primers. The nucleic acid composition for detecting diarrhea viruses can at least detect three of group A rotavirus, groupB rotavirus, group C rotavirus, norovirus, norovirus I type, norovirus II type, sapovirus, astrovirus and adenovirus simultaneously and has high detection sensitivity and good specificity.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a nucleic acid composition for detecting diarrhea virus, a kit and a method for using the kit. Background technique [0002] Diarrheal disease is an important global public health problem and one of the main diseases affecting human health around the world. It has widespread sporadic epidemics and outbreaks in both developing and developed countries. According to statistics, the annual incidence of children under the age of 5 in developing countries is about 750 to 1 billion, and about 5 million people die from diarrhea. Among various pathogens that cause diarrhea, viruses have become the most important type of diarrhea pathogens and the main cause of acute diarrhea. Common viruses that cause diarrhea include rotavirus, norovirus, sapovirus, astrovirus, and enteric adenovirus. Groups, B and C rotaviruses are predominant, and noroviruses are mainly types I and II. There are many se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/686C12N15/11C12R1/93
CPCC12Q1/686C12Q1/701C12Q2563/107C12Q2521/107
Inventor 罗成
Owner 深圳市刚竹医疗科技有限公司
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