36 results about "Diarrheal disease" patented technology

The invention discloses lactobacillus plantarum OBM7 and application thereof, and belongs to the technical field of biology. The strain is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on November 12, 2025, the preservation address is the 5th floor of the building 59, No.100 Courtyard, Xianlie Middle Road, Guangzhou, and the preservation number is GDMCC NO. 67282; and the probiotic preparation does not contain antibiotics or drug residues, and meets the development requirements of green and healthy breeding. The probiotic preparation can specifically prevent and control lamb diarrhea, significantly reduce the fatality rate of lamb diarrhea, effectively improve the survival rate of lambs, and provide targeted protection for lamb intestinal health; the lamb feed can regulate lamb gastrointestinal tract micro-ecological balance, improve digestion and absorption functions, significantly improve feed intake and feed conversion efficiency, and promote lamb growth performance improvement. Reliable technical support is provided for efficient, green and healthy breeding of lambs, high practicability and wide industrial application prospects are achieved, and the method has great significance in promoting green transformation of the livestock breeding industry.

The application belongs to the technical field of biotechnology, and particularly relates to a porcine epidemic diarrhea virus recombinant strain, a construction method and application. The S gene sequence of the existing epidemic porcine epidemic diarrhea virus PEDV CH / SX / 2016 strain is replaced by the S2015 gene sequence shown in SEQ ID NO. 1 to construct a PEDV CH / SX / 2016 strain infectious clone. A porcine epidemic diarrhea virus recombinant strain rCH / SX / 2016-S 2015 is successfully rescued by transfecting cells with a plasmid containing the infectious cDNA shown in SEQ ID NO. 4. The problem that the original strain cannot be successfully rescued by reverse genetics engineering is solved, and the recombinant virus strain rCH / SX / 2016-S 2015 has an activity comparable to that of the original strain, and can be used for preparing a PEDV diagnostic reagent and / or vaccine.

A vaccine comprising effective amounts of i) E. coli colonization factor antigens comprising CFA / I, CS3, CS5 and CS6 antigens; ii) a heat labile enterotoxin B subunit antigen being an LCTBA protein; and iii) an adjuvant being double mutant (R192G / L211A) of E. coli heat-labile toxin (dmLT), for use in inducing protective immunity against diarrheal disease caused by Salmonella spp. and / or Shigella spp.

The present invention describes immunogenic compositions containing immunogenic polypeptides of Porcine circovirus 2e, 3c, or 4 (PCV2e, PCV3c, or PCV4), including immunogenic compositions containing antigens other than PCV2e, PCV3c, or PCV4 antigens, including antigens that may be used in immunization against pathogens that cause diarrheal diseases. Methods of eliciting an immune response with the immunogenic compositions as disclosed and methods of treating a PCV2e, PCV3c, or PCV4 infection are also described.

The present invention relates to a strain of Lactobacillus johnsonii N5 and its application in preventing and treating enteritis and diarrhea. Lactobacillus johnsonii N5), was deposited in the China Center for Type Culture Collection on February 10, 2023, with the deposit number CCTCC NO: M2023104. The Lactobacillus johnsonii N5 was isolated from the fecal microorganisms of piglets resistant to heat stress-induced diarrhea. This Lactobacillus johnsonii N5 has the effect of alleviating enteritis and diarrhea, which is specifically reflected in: significantly enhancing the expression of intestinal epithelial tight junction proteins and cytoprotective heat shock protein 70, improving the immune balance of animal intestinal T cells, enhancing immune tolerance, and reducing the degree of intestinal inflammation and the occurrence of diarrheal diseases in animals. Therefore, Lactobacillus johnsonii N5 has a significant effect in preventing and treating diarrhea and related intestinal inflammatory diseases.

The invention belongs to the technical field of veterinary biological products, and relates to an mRNA freeze-dried vaccine of a porcine epidemic diarrhea virus as well as a preparation method and application of the mRNA freeze-dried vaccine. When the mRNA vaccine is prepared, firstly, the S gene sequences of the porcine epidemic diarrhea virus GIIa type and the porcine epidemic diarrhea virus GIIc type which are separated and stored in a laboratory are selected; the preparation method comprises the following steps: firstly, carrying out codon optimization, then respectively connecting with 5 'UTR, 3' UTR, a termination codon and a polyA tail nucleic acid sequence in series, connecting to a vector, carrying out in-vitro template transcription, carrying out in-vitro transcription, capping and purifying to obtain mRNA, and finally, encapsulating and freeze-drying to prepare the mRNA vaccine. The invention further provides application of the vaccine to immunization of pigs, cellular immunity and humoral immunity in the pigs can be stimulated, the immunogenicity and protective efficacy of the vaccine are superior to those of commercially available bivalent live vaccines, and the vaccine can be used for preventing porcine diarrhea caused by infection of currently epidemic PEDV GIIa and GIIc type strains.

The application discloses an oil and fat composition for resisting porcine enterovirus infection and application thereof. The oil and fat composition comprises at least two of glyceryl monopentanoate, glyceryl monolinoleate and glyceryl monomyristate. The oil and fat composition can further comprise anhydrous ethanol, propylene glycol, dimethyl sulfoxide, edible oil, Tween 80, lactose, mannitol, soluble starch, water and other auxiliary materials for preparing oil and fat composition preparations. The oil and fat composition can inhibit enterovirus through multiple channels and multiple levels, and improve the antiviral effect. Adding the oil and fat composition in the feed of pigs can not only improve the antiviral ability, but also reduce the inflammatory reaction caused by pathogenic infection through the anti-inflammatory and immune regulation effects, effectively prevent and treat the infection of porcine enterovirus diarrhea, and thus reduce the incidence of porcine enterovirus diarrhea and the mortality of pigs with the disease. The oil and fat composition can be used for preparing a medicine or a feed additive for treating and preventing porcine enterovirus diarrhea.

The present invention describes immunogenic compositions containing immunogenic polypeptides of Rotavirus (RV), including immunogenic compositions containing antigens other than RV antigens and antigens that may be used in immunization against pathogens that cause diarrheal diseases. Methods of eliciting an immune response with the immunogenic compositions as disclosed and methods of treating an RV infection are also described.

The invention provides a kit for detecting multiple piglet diarrhea pathogens and a detection method, and belongs to the technical field of biological information detection. The kit comprises a reaction reagent, wherein the reaction reagent comprises a porcine epidemic diarrhea virus specific primer and a probe, a porcine delta coronavirus specific primer and a probe, a piglet pathogenic escherichia coli specific primer and a probe, a clostridium perfringens specific primer and a probe, a buffer solution, an enzyme mixed solution and a freeze-drying protective additive. The detection method adopts the kit for detecting various piglet diarrhea pathogens. According to the kit disclosed by the invention, simultaneous detection of four target genes including the porcine epidemic diarrhea virus, the porcine delta coronavirus, the piglet pathogenic escherichia coli and the clostridium perfringens can be realized only through one-time amplification, so that the detection time is greatly shortened while the detection cost is reduced; the kit has extremely high specificity, sensitivity and repeatability, and the primers and the probes do not interfere with each other; good normal-temperature storage stability is also realized.

The present invention describes immunogenic compositions containing immunogenic polypeptides of Rabbit Haemorrhagic Disease Virus (RHDV), including immunogenic compositions containing antigens other than RHDV antigens, including antigens that may be used in immunization against pathogens that cause diarrheal diseases. Methods of eliciting an immune response with the immunogenic compositions as disclosed and methods of treating a RHDV infection are also described.

The invention discloses a fluorescent quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection primer and probe combination, a kit and a detection method for porcine viral diarrhea. Nucleotide sequences of detection primers are shown as SEQ ID NO.1-9, wherein the porcine viral diarrhea virus is porcine epidemic diarrhea virus PEDV, porcine group A rotavirus PoRVA and porcine delta coronavirus PDCoV. The detection primer or kit provided by the invention has remarkable application value in rapid detection of the porcine diarrhea virus and epidemiological monitoring of the porcine diarrhea virus.

This invention relates to the field of swine health monitoring technology and discloses a method for constructing a temporal attention network model for early warning of swine diarrhea. The method includes: acquiring and preprocessing swine physiological indicators and environmental factor data to generate standardized sequences; extracting features through deep learning to generate an initial feature vector set; labeling temporal weights to determine timestamp tags and dynamic weight coefficients; performing cross-modal temporal correlation analysis to generate the direction and strength of inter-modal correlations; collecting multimodal data to construct a feature matrix and performing temporal alignment; and mapping the aligned feature matrix to a health status knowledge graph to generate warning entries. This invention combines deep learning and temporal attention mechanisms to uncover deep correlations in multimodal data, improving the accuracy and efficiency of early warning and providing reliable technical support for swine health monitoring.

Disclosed herein are new salts and polymorphs of cethromycin for the treatment of diseases due to infection by bacteria and certain protozoans, including, for example, malaria, Babesosis, Toxoplasmosis, diarrheal disease, respiratory disease, sexually transmitted bacterial infections, and some bioterror bacteria, including, for example, plague, tularemia and post-inhalation anthrax. Also disclosed herein are new salts and polymorphs of cethromycin for the treatment of inflammatory diseases, including, for example, pelvic inflammatory disease, and peptic ulcer disease.

The present application relates to the technical field of molecular diagnosis, and discloses specific primers and a fluorescent PCR detection method for identifying Vibrio parahaemolyticus KUT2 type strains, and provides primers KUT2-F / R and a TaqMan probe for identifying Vibrio parahaemolyticus KUT2 type strains, and establishes a fluorescent PCR method (TaqMan probe method). The present application can quickly identify the main Vibrio parahaemolyticus KUT type prevailing in diarrhea patients in China, expand the Vibrio parahaemolyticus serotyping system, and provide a more accurate tool for epidemiological investigation and tracing. The present application has reached the level of the fluorescent PCR technology in terms of amplification efficiency, sensitivity and specificity, thereby realizing efficient and specific detection of KUT strains, and can judge whether the strain is the main KUT type prevailing in China within 1 hour, and fully meets the actual use detection requirements.

The invention relates to the technical field of animal disease prevention and treatment, in particular to a comprehensive treatment system and method for yak diarrhea based on a Tibetan medicine compound.The method comprises the steps that a yak excrement sample is collected, and the diarrhea pathogen type is judged through multiple PCR detection; calling a corresponding compound proportion in a Tibetan medicine compound database according to a judgment result, and blending a powdery compound composition consisting of herpetospermum pedunculosum, rhododendron parvifolium, pterocephalus hookeri and herba aconiti; dynamically extracting with a low-temperature buffer solution, mixing with lactobacillus plantarum freeze-dried powder, and preparing a sustained-release microcapsule through a sodium alginate-chitosan two-phase embedding technology; the yaks with diarrhea are drenched with the micro-capsules firstly, and then the remaining micro-capsules are mixed with fermented highland barley feed in proportion to be continuously fed. According to the invention, the integrated treatment process of pathogen recognition, Tibetan medicine matching, bacterial medicine synergy and continuous administration is realized, the curative effect, compliance and Tibetan medicine application stability are effectively improved, and the Tibetan medicine is suitable for scientific prevention and treatment of diarrhea of yaks in plateau pasturing areas.

The present invention describes immunogenic compositions containing immunogenic polypeptides of Avian Influenza Virus (IAV), including immunogenic compositions containing antigens other than IAV antigens, including antigens that may be used in immunization against pathogens that cause diarrheal diseases. Methods of eliciting an immune response with the immunogenic compositions as disclosed and methods of treating an IAV infection are also described.

The present invention describes immunogenic compositions containing immunogenic polypeptides of Porcine circovirus 2e, 3c, or 4 (PCV2e, PCV3c, or PCV4), including immunogenic compositions containing antigens other than PCV2e, PCV3c, or PCV4 antigens, including antigens that may be used in immunization against pathogens that cause diarrheal diseases. Methods of eliciting an immune response with the immunogenic compositions as disclosed and methods of treating a PCV2e, PCV3c, or PCV4 infection are also described.

The application provides a pig getah virus positive serum and a preparation method and application, and belongs to the technical field of animal virology. The application provides a preparation method of a pig getah virus positive serum, wherein a CDCD pig is used as a host, neutralizing antibody titer obtained through multiple immunization of high-concentration antigen is greater than or equal to 1:512; the serum does not have cytotoxicity affecting cell culture; the CDCD pig is a pig free from maternal antibody interference, which is obtained through caesarean section, isolated feeding and artificial feeding; the positive serum does not detect pig pseudorabies virus, swine fever virus, porcine circovirus type 2, pig foot-and-mouth disease virus, bovine viral diarrhea virus, porcine infectious gastroenteritis virus, porcine epidemic diarrhea virus, porcine rotavirus, porcine parvovirus, porcine Japanese encephalitis virus antibody, and has the characteristics of high titer and no cytotoxicity.

Described herein are compositions, methods, and kits for detecting diarrhea-causing pathogens from a patient, food, or environmental sample. One embodiment described herein is a primer pair and probe for a multiplex polymerase chain reaction (PCR)-based assay for the detection of diarrhea-causing pathogens, such as Campylobacter spp., Salmonella spp., Shigella spp. / Enteroinvasive Escherichia coli (EIEC), Escherichia coli stx1 / Shiga toxin A, or Escherichia coli stx2 / Shiga toxin B. Other embodiments include methods and kits for detecting diarrhea-causing pathogens.

The invention discloses a composition and feed for preventing lamb diarrhea, and relates to the technical field of lamb feeds, the composition comprises the following raw materials by weight: 6%-10% of bee venom antibacterial peptide; 1%-3% of lactein; 2%-5% of phage freeze-dried powder; 4%-6% of tannic acid; 30%-40% of glycolysate montmorillonite; 1%-3% of a milk fragrance agent; 1%-3% of a sweetening agent; 8%-11% of a flavor enhancer; and 25%-30% of yeast hydrolysate. According to the technical scheme, a quadruple integrated technical system is created for the first time; four innovation dimensions realize the crossing of lamb diarrhea prevention and treatment from passive treatment to active defense; in the antibacterial dimension, bee venom antibacterial peptide and bacteriophage break through a biological membrane barrier; according to the barrier dimension, tannic acid and glycolysate montmorillonite are used for constructing double shields; according to the ecological dimension, lactein and yeast hydrolysate drive flora balance; and metabolism dimension: forcible ingestion of a flavoring agent and nutrition restoration of a flavor enhancer are combined, so that malnutrition secondary infection is blocked. And in a verification experiment, the diarrhea approaching zero is realized, and the clearance rate of pathogenic bacteria is greater than 99.99% (100 times higher than that of a double-component group).

The invention relates to the technical field of live pig health monitoring, and discloses a time sequence attention network model construction method for early warning of live pig diarrhea, which comprises the following steps: acquiring live pig physiological indexes and environmental factor data, and preprocessing to generate a standardized sequence; extracting features through deep learning to generate an initial feature vector set; marking a time sequence weight to determine a timestamp label and a dynamic weight coefficient; performing cross-modal time sequence correlation analysis to generate inter-modal correlation direction and strength; collecting multi-modal data to construct a feature matrix and performing time sequence alignment; and mapping the alignment feature matrix to a health state knowledge graph to generate an early warning entry. According to the method, deep learning and a time sequence attention mechanism can be combined, multi-modal data deep association is mined, the early warning precision and efficiency are improved, and reliable technical support is provided for live pig health monitoring.

The invention relates to the technical field of biological medicine, in particular to a construction method and application of an anti-porcine diarrhea virus recombinant cell strain. Aiming at the problems of low PEDV S protein expression efficiency and insufficient cell strain stability in the prior art, the invention aims to provide a construction method and application of a recombinant cell strain for resisting porcine diarrhea virus, the recombinant CHO cell strain capable of efficiently secreting PEDV S protein is obtained by optimizing expression vector design, a screening strategy and a suspension domestication process, and the recombinant CHO cell strain can be used for efficiently secreting PEDV S protein. And a foundation is laid for preparation of subunit vaccines and detection reagents.

The invention belongs to the field of molecular biological detection, and particularly relates to a multiplex fluorescent PCR (polymerase chain reaction) detection kit for children-induced diarrhea virus and application thereof, the kit is internally provided with a mixture of primer groups and probe sequences for detecting group A rotavirus, norovirus type GII and enteroadenovirus type 40 / 41, meanwhile, the kit is also provided with a primer and a probe sequence for detecting a human endogenous reference gene Homo RNase P; the primer sequences for detecting the group A rotavirus are respectively as shown in SEQ ID NO: 1 and SEQ ID NO: 2, the Taqman probe sequence is as shown in SEQ ID NO: 3, and the fluorescence mark is FAM. The kit has very high sensitivity and specificity, realizes rapid and synchronous detection and analysis of clinically common children-induced diarrhea viruses, can effectively improve the detection efficiency, provides comprehensive and accurate etiological diagnosis for clinic, and has a very good application prospect.

The present invention relates to an antimicrobial composition for treating a foodborne illness comprising a combination of a polar extract of Jamaica (hibiscus) with a broad-spectrum antibiotic and at least one additive agent. Mixtures of the compounds in the formulations at specific concentrations exhibit an antimicrobial and protective effect against antibiotic-resistant bacteria.

The present invention describes immunogenic compositions containing immunogenic polypeptides of Avian Influenza Virus (IAV), including immunogenic compositions containing antigens other than IAV antigens, including antigens that may be used in immunization against pathogens that cause diarrheal diseases. Methods of eliciting an immune response with the immunogenic compositions as disclosed and methods of treating an IAV infection are also described.

The invention belongs to the technical field of molecular biological detection, and particularly relates to a bovine diarrhea pathogenic bacterium triple PCR detection primer group, a kit and a detection method. The invention discloses a triple PCR (Polymerase Chain Reaction) detection primer group for three bovine diarrhea pathogenic bacteria causing bovine viral diarrhea mucosal disease virus, bovine rotavirus and bovine cobavirus, the sequences of the primer group are shown as SEQ ID NO: 1-6, and the invention discloses a triple PCR detection kit containing the primer group for the bovine diarrhea pathogenic bacteria and a detection method. Compared with the prior art, the detection method has the characteristics of high detection efficiency, low cost, strong specificity, good repeatability and high detection coincidence rate, is suitable for clinical high-throughput rapid diagnosis, and provides guarantee for prevention and control of pathogenic bovine diarrhea and reduction of economic loss.

1. Name of the product in this design: Stool sampling tube for patients with diarrhea. 2. Purpose of this design: As a sampling tube for collecting stool samples from patients with diarrhea. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1.

The invention relates to application of Liushen pills in treating diseases caused by various virus infections. The inventor finds that the Liushen pill has a good inhibition effect on various viruses, especially Coxsackie virus A type 9, respiratory syncytial virus, adenovirus C type 5, vaccinia virus, fever with thrombocytopenia syndrome bunyavirus, porcine epidemic diarrhea virus, pangolin coronavirus, Zika virus and Japanese encephalitis virus. In view of the strong inhibition effect on various viruses such as poxvirus, enterovirus, respiratory syncytial virus, adenovirus, bunyavirus, coronavirus and flavivirus observed by the Liushen pill, the Liushen pill is likely to become a specific medicine for diseases caused by infection of various viruses.

The invention relates to the technical field of molecular diagnosis, and discloses a specific primer for identifying a vibrio parahaemolyticus KUT1 type strain and a fluorescent PCR (Polymerase Chain Reaction) detection method. The invention provides a primer KUT1-F / R and a TaqMan probe for identifying a vibrio parahaemolyticus KUT1 type strain, and establishes a fluorescent PCR (Polymerase Chain Reaction) method (TaqMan probe method). According to the method, the KUT type of the main vibrio parahaemolyticus epidemic in diarrhea patients in China can be quickly identified, a serotyping system of the vibrio parahaemolyticus is expanded, and a more accurate tool is provided for epidemiological investigation and traceability. Compared with the prior art, the kit has the advantages that the due level of a fluorescent PCR technology is achieved in the aspects of amplification efficiency, sensitivity and specificity, so that efficient and specific detection on the KUT strain is realized, whether the strain is the mainly popular KUT type in China or not can be judged within one hour, and the actual use detection requirement is fully met.

The present invention relates to the field of molecular biology detection technology, and in particular to a primer and probe set, a kit and an application for multiple nucleic acid mass spectrometry detection of swine diarrhea pathogens. The present invention adopts matrix-assisted laser desorption / ionization time-of-flight nucleic acid mass spectrometry technology, which can simultaneously detect eight major pathogens such as PEDV, TGEV, PDCoV, PoRV, SADS-CoV, PBoV, HEV and Salmonella in a rapid, sensitive and specific manner. By designing specific primers and unextended probes, combined with multiple PCR amplification and mass spectrometry analysis, the present method achieves high-throughput, simultaneous detection of multiple pathogens. Experimental results show that the method has high sensitivity and high specificity, no false positive reactions, and shows 100% repeatability in 60 repeated experiments. Comparison with the qPCR method shows that the detection consistency is as high as 96.2%. The method has the advantages of simple operation, fast detection speed and strong adaptability. It can be widely used in the efficient monitoring of swine diarrhea pathogens, providing important technical support for the early diagnosis and prevention and control of swine diseases.