50 results about "Diarrheal disease" patented technology

The present invention "integrated POCT test kit, test tube and test system for detecting 15 diarrhea pathogens" belongs to the field of biomedical testing. The test kit includes: fecal sample pretreatment reagents and pathogen detection reagents; the fecal sample pretreatment reagents include: 1~4M guanidine salt, 20~200mM sodium citrate, 1~10% SDS by volume, 2~10% polidocanol by volume, 25~50% isopropanol by volume, etc., 10~30mg / mL nanocarbon, 10~30mg / mL zeolite powder; pathogen detection reagents include system A, system B, system C, and system D reagents. The present invention also provides a test tube and a test system based on the test kit. The present invention can not only detect 15 pathogens at the same time, but also achieve the purpose of integrated POCT detection with good repeatability of test results, large test sample size, high sensitivity, good specificity, good test timeliness, and sample-in and result-out.

The invention relates to the technical field of molecular biological detection, in particular to a primer, a probe group and a kit for multiplex nucleic acid mass spectrometry detection of porcine diarrhea pathogens and application. By adopting a matrix-assisted laser desorption / ionization time-of-flight nucleic acid mass spectrometry technology, eight main pathogens such as PEDV, TGEV, PDCoV, PoRV, SADS-CoV, PBoV, HEV and salmonella can be rapidly, sensitively and specifically detected at the same time. By designing the specific primer and the unexpanded probe and combining multiple PCR amplification and mass spectrometry, the method realizes high-throughput and multi-pathogen simultaneous detection. Experimental results show that the method has high sensitivity and high specificity, has no false positive reaction, and shows 100% repeatability in 60 repeated experiments. Compared with a qPCR method, the detection consistency is as high as 96.2%. The method has the advantages of being easy and convenient to operate, high in detection speed and high in adaptability, can be widely applied to efficient monitoring of porcine diarrhea pathogens, and provides important technical support for early diagnosis, prevention and control of porcine diseases.

The invention discloses lactobacillus plantarum OBM7 and application thereof, and belongs to the technical field of biology. The strain is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on November 12, 2025, the preservation address is the 5th floor of the building 59, No.100 Courtyard, Xianlie Middle Road, Guangzhou, and the preservation number is GDMCC NO. 67282; and the probiotic preparation does not contain antibiotics or drug residues, and meets the development requirements of green and healthy breeding. The probiotic preparation can specifically prevent and control lamb diarrhea, significantly reduce the fatality rate of lamb diarrhea, effectively improve the survival rate of lambs, and provide targeted protection for lamb intestinal health; the lamb feed can regulate lamb gastrointestinal tract micro-ecological balance, improve digestion and absorption functions, significantly improve feed intake and feed conversion efficiency, and promote lamb growth performance improvement. Reliable technical support is provided for efficient, green and healthy breeding of lambs, high practicability and wide industrial application prospects are achieved, and the method has great significance in promoting green transformation of the livestock breeding industry.

The present invention relates to a diagnostic kit for bovine viral diarrhea virus (BVDV), which enables rapid on-site identification and diagnosis of BVDV1 and BVDV2, which are genotypes of the BVD virus that are pathogens causing bovine diarrhea, within a short time of approximately 15 minutes.

The application belongs to the technical field of biotechnology, and particularly relates to a porcine epidemic diarrhea virus recombinant strain, a construction method and application. The S gene sequence of the existing epidemic porcine epidemic diarrhea virus PEDV CH / SX / 2016 strain is replaced by the S2015 gene sequence shown in SEQ ID NO. 1 to construct a PEDV CH / SX / 2016 strain infectious clone. A porcine epidemic diarrhea virus recombinant strain rCH / SX / 2016-S 2015 is successfully rescued by transfecting cells with a plasmid containing the infectious cDNA shown in SEQ ID NO. 4. The problem that the original strain cannot be successfully rescued by reverse genetics engineering is solved, and the recombinant virus strain rCH / SX / 2016-S 2015 has an activity comparable to that of the original strain, and can be used for preparing a PEDV diagnostic reagent and / or vaccine.

The invention discloses a grease composition for resisting porcine envelope virus infection and application of the grease composition. The grease composition comprises at least two of glyceryl monovalerate, glyceryl monolinoleate and glyceryl monomyristate. The grease composition can also comprise absolute ethyl alcohol, propylene glycol, dimethyl sulfoxide, edible oil, lactose in Tween 80, mannitol, soluble starch, water and other auxiliary materials, and is used for preparing a grease composition preparation. The grease composition can inhibit envelop viruses in a multi-way and multi-layer mode, and the antiviral effect is improved. By adding the grease composition into pig feed, not only can the antiviral capability be improved, but also inflammatory response caused by pathogenic infection can be reduced through the anti-inflammatory and immune regulation effects of the grease composition, and the infection of swine envelope viral diarrhea can be effectively prevented and treated, so that the incidence rate of the swine envelope viral diarrhea and the death rate of diseased pigs are reduced. The grease composition can be used for preparing a medicine or a feed additive for treating and preventing swine envelope viral diarrhea.

The invention discloses a quadruple PCR (Polymerase Chain Reaction) detection primer combination for detecting diarrhea pathogenic bacteria of young deer and application, and belongs to the technical field of biology. The quadruple PCR detection primer combination for detecting the diarrhea pathogenic bacteria of the young deer comprises an escherichia coli primer pair as shown in SEQ ID NO.1-2, a klebsiella pneumoniae primer pair as shown in SEQ ID NO.3-4, a salmonella primer pair as shown in SEQ ID NO.5-6 and a proteus mirabilis primer pair as shown in SEQ ID NO.7-8. The invention further discloses a kit for detecting the diarrhea pathogenic bacteria of the young deer. The multiple PCR detection method is established by using the specific genes of the main pathogenic bacteria of the diarrhea of the baby deer, so that the pathogenesis of the diarrhea of the baby deer can be quickly and accurately detected, the detection efficiency of epidemic diseases is greatly improved, and the method has important significance in preventing and treating bacterial diarrhea of the baby deer.

A vaccine comprising effective amounts of i) E. coli colonization factor antigens comprising CFA / I, CS3, CS5 and CS6 antigens; ii) a heat labile enterotoxin B subunit antigen being an LCTBA protein; and iii) an adjuvant being double mutant (R192G / L211A) of E. coli heat-labile toxin (dmLT), for use in inducing protective immunity against diarrheal disease caused by Salmonella spp. and / or Shigella spp.

The present invention describes immunogenic compositions containing immunogenic polypeptides of Porcine circovirus 2e, 3c, or 4 (PCV2e, PCV3c, or PCV4), including immunogenic compositions containing antigens other than PCV2e, PCV3c, or PCV4 antigens, including antigens that may be used in immunization against pathogens that cause diarrheal diseases. Methods of eliciting an immune response with the immunogenic compositions as disclosed and methods of treating a PCV2e, PCV3c, or PCV4 infection are also described.

The present invention relates to a strain of Lactobacillus johnsonii N5 and its application in preventing and treating enteritis and diarrhea. Lactobacillus johnsonii N5), was deposited in the China Center for Type Culture Collection on February 10, 2023, with the deposit number CCTCC NO: M2023104. The Lactobacillus johnsonii N5 was isolated from the fecal microorganisms of piglets resistant to heat stress-induced diarrhea. This Lactobacillus johnsonii N5 has the effect of alleviating enteritis and diarrhea, which is specifically reflected in: significantly enhancing the expression of intestinal epithelial tight junction proteins and cytoprotective heat shock protein 70, improving the immune balance of animal intestinal T cells, enhancing immune tolerance, and reducing the degree of intestinal inflammation and the occurrence of diarrheal diseases in animals. Therefore, Lactobacillus johnsonii N5 has a significant effect in preventing and treating diarrhea and related intestinal inflammatory diseases.

The invention belongs to the technical field of veterinary biological products, and relates to an mRNA freeze-dried vaccine of a porcine epidemic diarrhea virus as well as a preparation method and application of the mRNA freeze-dried vaccine. When the mRNA vaccine is prepared, firstly, the S gene sequences of the porcine epidemic diarrhea virus GIIa type and the porcine epidemic diarrhea virus GIIc type which are separated and stored in a laboratory are selected; the preparation method comprises the following steps: firstly, carrying out codon optimization, then respectively connecting with 5 'UTR, 3' UTR, a termination codon and a polyA tail nucleic acid sequence in series, connecting to a vector, carrying out in-vitro template transcription, carrying out in-vitro transcription, capping and purifying to obtain mRNA, and finally, encapsulating and freeze-drying to prepare the mRNA vaccine. The invention further provides application of the vaccine to immunization of pigs, cellular immunity and humoral immunity in the pigs can be stimulated, the immunogenicity and protective efficacy of the vaccine are superior to those of commercially available bivalent live vaccines, and the vaccine can be used for preventing porcine diarrhea caused by infection of currently epidemic PEDV GIIa and GIIc type strains.

The application discloses an oil and fat composition for resisting porcine enterovirus infection and application thereof. The oil and fat composition comprises at least two of glyceryl monopentanoate, glyceryl monolinoleate and glyceryl monomyristate. The oil and fat composition can further comprise anhydrous ethanol, propylene glycol, dimethyl sulfoxide, edible oil, Tween 80, lactose, mannitol, soluble starch, water and other auxiliary materials for preparing oil and fat composition preparations. The oil and fat composition can inhibit enterovirus through multiple channels and multiple levels, and improve the antiviral effect. Adding the oil and fat composition in the feed of pigs can not only improve the antiviral ability, but also reduce the inflammatory reaction caused by pathogenic infection through the anti-inflammatory and immune regulation effects, effectively prevent and treat the infection of porcine enterovirus diarrhea, and thus reduce the incidence of porcine enterovirus diarrhea and the mortality of pigs with the disease. The oil and fat composition can be used for preparing a medicine or a feed additive for treating and preventing porcine enterovirus diarrhea.

The present invention describes immunogenic compositions containing immunogenic polypeptides of Rotavirus (RV), including immunogenic compositions containing antigens other than RV antigens and antigens that may be used in immunization against pathogens that cause diarrheal diseases. Methods of eliciting an immune response with the immunogenic compositions as disclosed and methods of treating an RV infection are also described.

The invention provides a kit for detecting multiple piglet diarrhea pathogens and a detection method, and belongs to the technical field of biological information detection. The kit comprises a reaction reagent, wherein the reaction reagent comprises a porcine epidemic diarrhea virus specific primer and a probe, a porcine delta coronavirus specific primer and a probe, a piglet pathogenic escherichia coli specific primer and a probe, a clostridium perfringens specific primer and a probe, a buffer solution, an enzyme mixed solution and a freeze-drying protective additive. The detection method adopts the kit for detecting various piglet diarrhea pathogens. According to the kit disclosed by the invention, simultaneous detection of four target genes including the porcine epidemic diarrhea virus, the porcine delta coronavirus, the piglet pathogenic escherichia coli and the clostridium perfringens can be realized only through one-time amplification, so that the detection time is greatly shortened while the detection cost is reduced; the kit has extremely high specificity, sensitivity and repeatability, and the primers and the probes do not interfere with each other; good normal-temperature storage stability is also realized.

The present invention describes immunogenic compositions containing immunogenic polypeptides of Rabbit Haemorrhagic Disease Virus (RHDV), including immunogenic compositions containing antigens other than RHDV antigens, including antigens that may be used in immunization against pathogens that cause diarrheal diseases. Methods of eliciting an immune response with the immunogenic compositions as disclosed and methods of treating a RHDV infection are also described.

The invention discloses a fluorescent quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection primer and probe combination, a kit and a detection method for porcine viral diarrhea. Nucleotide sequences of detection primers are shown as SEQ ID NO.1-9, wherein the porcine viral diarrhea virus is porcine epidemic diarrhea virus PEDV, porcine group A rotavirus PoRVA and porcine delta coronavirus PDCoV. The detection primer or kit provided by the invention has remarkable application value in rapid detection of the porcine diarrhea virus and epidemiological monitoring of the porcine diarrhea virus.

This invention relates to the field of swine health monitoring technology and discloses a method for constructing a temporal attention network model for early warning of swine diarrhea. The method includes: acquiring and preprocessing swine physiological indicators and environmental factor data to generate standardized sequences; extracting features through deep learning to generate an initial feature vector set; labeling temporal weights to determine timestamp tags and dynamic weight coefficients; performing cross-modal temporal correlation analysis to generate the direction and strength of inter-modal correlations; collecting multimodal data to construct a feature matrix and performing temporal alignment; and mapping the aligned feature matrix to a health status knowledge graph to generate warning entries. This invention combines deep learning and temporal attention mechanisms to uncover deep correlations in multimodal data, improving the accuracy and efficiency of early warning and providing reliable technical support for swine health monitoring.

The present invention pertains to a novel rotavirus, especially an isolated virus, which is a member of the sub-species of porcine group B rotavirus (porcine RVB), causing diarrheal disease in pigs, to DNA fragments and corresponding proteins of the said virus, to vaccines on the basis of said virus, DNA and / or protein and to antibodies reactive with said virus and / or protein and diagnostic test kits for the detection of said virus.

Disclosed herein are new salts and polymorphs of cethromycin for the treatment of diseases due to infection by bacteria and certain protozoans, including, for example, malaria, Babesosis, Toxoplasmosis, diarrheal disease, respiratory disease, sexually transmitted bacterial infections, and some bioterror bacteria, including, for example, plague, tularemia and post-inhalation anthrax. Also disclosed herein are new salts and polymorphs of cethromycin for the treatment of inflammatory diseases, including, for example, pelvic inflammatory disease, and peptic ulcer disease.

The invention provides a primer probe group, a kit and a detection method for detecting diarrhea pathogens of children based on multiple PCR (Polymerase Chain Reaction) combined with time-of-flight mass spectrometry. The primer probe group comprises specific primers and probes aiming at 10 diarrhea pathogens (5 bacteria and 5 viruses) and a human-derived reference gene. Specific primers and probes are designed in a pathogen gene conserved region, targeted detection is achieved, and specificity is high; 10 diarrhea-causing pathogens and a human-derived reference gene in an excrement sample can be simultaneously detected in the same PCR reaction system and the single-base extension system, the sensitivity and the accuracy are high, and the detection flux is improved by combining a full-automatic chip spotting instrument; the cost is low, the detection period is short, and reliable technical support is provided for diagnosis and treatment, epidemic situation monitoring and infection control of children with infectious diarrhea.

The present application relates to the technical field of molecular diagnosis, and discloses specific primers and a fluorescent PCR detection method for identifying Vibrio parahaemolyticus KUT2 type strains, and provides primers KUT2-F / R and a TaqMan probe for identifying Vibrio parahaemolyticus KUT2 type strains, and establishes a fluorescent PCR method (TaqMan probe method). The present application can quickly identify the main Vibrio parahaemolyticus KUT type prevailing in diarrhea patients in China, expand the Vibrio parahaemolyticus serotyping system, and provide a more accurate tool for epidemiological investigation and tracing. The present application has reached the level of the fluorescent PCR technology in terms of amplification efficiency, sensitivity and specificity, thereby realizing efficient and specific detection of KUT strains, and can judge whether the strain is the main KUT type prevailing in China within 1 hour, and fully meets the actual use detection requirements.

The invention relates to the technical field of animal disease prevention and treatment, in particular to a comprehensive treatment system and method for yak diarrhea based on a Tibetan medicine compound.The method comprises the steps that a yak excrement sample is collected, and the diarrhea pathogen type is judged through multiple PCR detection; calling a corresponding compound proportion in a Tibetan medicine compound database according to a judgment result, and blending a powdery compound composition consisting of herpetospermum pedunculosum, rhododendron parvifolium, pterocephalus hookeri and herba aconiti; dynamically extracting with a low-temperature buffer solution, mixing with lactobacillus plantarum freeze-dried powder, and preparing a sustained-release microcapsule through a sodium alginate-chitosan two-phase embedding technology; the yaks with diarrhea are drenched with the micro-capsules firstly, and then the remaining micro-capsules are mixed with fermented highland barley feed in proportion to be continuously fed. According to the invention, the integrated treatment process of pathogen recognition, Tibetan medicine matching, bacterial medicine synergy and continuous administration is realized, the curative effect, compliance and Tibetan medicine application stability are effectively improved, and the Tibetan medicine is suitable for scientific prevention and treatment of diarrhea of yaks in plateau pasturing areas.

The present invention provides a primer probe set, reagent and detection method for detecting diarrhea pathogens in children based on digital PCR. The primer probe set includes specific primers and probes for 10 diarrhea pathogens and human internal reference genes; primers and probes for 5 bacteria and primers and probes for human internal reference genes are designed in the same PCR reaction system, and primers and probes for 5 viruses are designed in another PCR reaction system. The present invention designs specific primers and probes in the conservative region of pathogen genes, and has targeted detection and strong specificity; 10 diarrhea pathogens in stool specimens can be quickly detected in two PCR reaction systems, with high sensitivity, greatly reducing costs and shortening detection time, solving the pain point of conventional PCR in detecting multiple pathogens in stool specimens at the same time, overcoming the difficulty of being easily interfered by inhibitors in stool specimen detection, and providing a reliable laboratory basis for diagnosis and treatment of children, epidemic monitoring and infection control.

The present invention describes immunogenic compositions containing immunogenic polypeptides of Avian Influenza Virus (IAV), including immunogenic compositions containing antigens other than IAV antigens, including antigens that may be used in immunization against pathogens that cause diarrheal diseases. Methods of eliciting an immune response with the immunogenic compositions as disclosed and methods of treating an IAV infection are also described.

The present invention describes immunogenic compositions containing immunogenic polypeptides of Porcine circovirus 2e, 3c, or 4 (PCV2e, PCV3c, or PCV4), including immunogenic compositions containing antigens other than PCV2e, PCV3c, or PCV4 antigens, including antigens that may be used in immunization against pathogens that cause diarrheal diseases. Methods of eliciting an immune response with the immunogenic compositions as disclosed and methods of treating a PCV2e, PCV3c, or PCV4 infection are also described.

The application provides a pig getah virus positive serum and a preparation method and application, and belongs to the technical field of animal virology. The application provides a preparation method of a pig getah virus positive serum, wherein a CDCD pig is used as a host, neutralizing antibody titer obtained through multiple immunization of high-concentration antigen is greater than or equal to 1:512; the serum does not have cytotoxicity affecting cell culture; the CDCD pig is a pig free from maternal antibody interference, which is obtained through caesarean section, isolated feeding and artificial feeding; the positive serum does not detect pig pseudorabies virus, swine fever virus, porcine circovirus type 2, pig foot-and-mouth disease virus, bovine viral diarrhea virus, porcine infectious gastroenteritis virus, porcine epidemic diarrhea virus, porcine rotavirus, porcine parvovirus, porcine Japanese encephalitis virus antibody, and has the characteristics of high titer and no cytotoxicity.

The present invention pertains to a novel rotavirus, especially an isolated virus, which is a member of the sub-species of porcine group B rotavirus (porcine RVB), causing diarrheal disease in pigs, to DNA fragments and corresponding proteins of the said virus, to vaccines on the basis of said virus, DNA and / or protein and to antibodies reactive with said virus and / or protein and diagnostic test kits for the detection of said virus.

Described herein are compositions, methods, and kits for detecting diarrhea-causing pathogens from a patient, food, or environmental sample. One embodiment described herein is a primer pair and probe for a multiplex polymerase chain reaction (PCR)-based assay for the detection of diarrhea-causing pathogens, such as Campylobacter spp., Salmonella spp., Shigella spp. / Enteroinvasive Escherichia coli (EIEC), Escherichia coli stx1 / Shiga toxin A, or Escherichia coli stx2 / Shiga toxin B. Other embodiments include methods and kits for detecting diarrhea-causing pathogens.

The invention discloses a composition and feed for preventing lamb diarrhea, and relates to the technical field of lamb feeds, the composition comprises the following raw materials by weight: 6%-10% of bee venom antibacterial peptide; 1%-3% of lactein; 2%-5% of phage freeze-dried powder; 4%-6% of tannic acid; 30%-40% of glycolysate montmorillonite; 1%-3% of a milk fragrance agent; 1%-3% of a sweetening agent; 8%-11% of a flavor enhancer; and 25%-30% of yeast hydrolysate. According to the technical scheme, a quadruple integrated technical system is created for the first time; four innovation dimensions realize the crossing of lamb diarrhea prevention and treatment from passive treatment to active defense; in the antibacterial dimension, bee venom antibacterial peptide and bacteriophage break through a biological membrane barrier; according to the barrier dimension, tannic acid and glycolysate montmorillonite are used for constructing double shields; according to the ecological dimension, lactein and yeast hydrolysate drive flora balance; and metabolism dimension: forcible ingestion of a flavoring agent and nutrition restoration of a flavor enhancer are combined, so that malnutrition secondary infection is blocked. And in a verification experiment, the diarrhea approaching zero is realized, and the clearance rate of pathogenic bacteria is greater than 99.99% (100 times higher than that of a double-component group).

The invention relates to the technical field of live pig health monitoring, and discloses a time sequence attention network model construction method for early warning of live pig diarrhea, which comprises the following steps: acquiring live pig physiological indexes and environmental factor data, and preprocessing to generate a standardized sequence; extracting features through deep learning to generate an initial feature vector set; marking a time sequence weight to determine a timestamp label and a dynamic weight coefficient; performing cross-modal time sequence correlation analysis to generate inter-modal correlation direction and strength; collecting multi-modal data to construct a feature matrix and performing time sequence alignment; and mapping the alignment feature matrix to a health state knowledge graph to generate an early warning entry. According to the method, deep learning and a time sequence attention mechanism can be combined, multi-modal data deep association is mined, the early warning precision and efficiency are improved, and reliable technical support is provided for live pig health monitoring.