59 results about "Gene mining" patented technology

The present invention provides a system, method and apparatus for targeting gene sequences having one or more phenotypic characteristics using a computer. One or more phenotypic characteristics are selected. A gene sequence is then selected that is known to have the selected phenotypic characteristics. In addition, one or more databases containing cataloged gene sequences are selected. The selected gene sequence is compared to the cataloged gene sequences, and any cataloged gene sequences that contain a portion of the selected gene sequence are extracted. The selected gene sequence is aligned to each portion of the extracted gene sequence and the extracted gene sequences are prioritized based on the alignment of the selected gene sequence. At least one of the prioritized gene sequences is selected based on one or more phenotypic criteria. Finally, one or more degenerate primers are designed to target the selected-prioritized gene sequences.

The invention provides a tumor mutation site screening and mutual exclusion gene mining system. The system comprises a filtering module which is used for filtering a vcf file and an ANNOVAR software output file in an exome processing process; an analysis module which is used for carrying out description analysis on different experiment group mutation sites; a gathering module which is used for gathering mutation genes of each sample and establishing a mutation gene matrix according to an experiment group mutation gene list; and a mining module which is used for carrying out Fisher precise checking based mutual exclusion and co-mutation analysis on the generated mutation gene matrix, thereby determining mutual exclusion and co-mutation genes. According to system, the mutation sites are filtered according to basic parameters such as annotation information of the mutation sites, the sequencing read number and site sequencing depth; and description analysis of different experiment group mutation modes and mining of co-mutation and mutual exclusion gene sets are carried out on the obtained mutation sites.

The invention relates to rapid identification of anti-powdery-mildew gene of Brachypodium distachyon, relates to the knowledge of plant comparative genomics, genetics, biological information and other subjects, and belongs to the scientific field of plant biotechnology. The rapid identification of anti-powdery-mildew gene of Brachypodium distachyon comprises the main steps of: (1) downloading full genome sequences of Brachypodium distachyon and collecting MLO (mildew resistance locus o) type gene; (2) identifying the MLO type gene; (3) analyzing the MLO type gene historical development relation; and (4) comparing the MLO type powdery mildew gene. According to the rapid identification of anti-powdery-mildew gene of Brachypodium distachyon provided by the invention, the excavation period of the anti-powdery-mildew gene of Brachypodium distachyon is effectively shortened, and the rapid identification of the powdery mildew gene is facilitated; the identified powdery mildew gene can be used for developing corresponding coseparation functional markers (SNP (single nucleotide polymorphism), SCAR (sequence-characterized amplified region), and the like), and can also be rapidly used for assisted selection of molecule markers of anti-powdery-mildew gene, and the accuracy is high; development of multi-resistance breeding materials can be carried out by combining with other anti-disease gene molecule markers, the breeding time is shortened and the breeding efficiency is improved; and foundation is established for expounding the molecular mechanism of the anti-powdery-mildew gene of Brachypodium distachyon.

The present invention provides a system, method and apparatus for targeting gene sequences having one or more phenotypic characteristics using a computer. One or more phenotypic characteristics are selected. A gene sequence is then selected that is known to have the selected phenotypic characteristics. In addition, one or more databases containing cataloged gene sequences are selected. The selected gene sequence is compared to the cataloged gene sequences, and any cataloged gene sequences that contain a portion of the selected gene sequence are extracted. The selected gene sequence is aligned to each portion of the extracted gene sequence and the extracted gene sequences are prioritized based on the alignment of the selected gene sequence. At least one of the prioritized gene sequences is selected based on one or more phenotypic criteria. Finally, one or more degenerate primers are designed to target the selected-prioritized gene sequences.

The invention provides rapid identification of soybean anti-powdery mildew gene, relates to knowledge in the disciplines of plant comparative genomics, genetics and bioinformatics, and belongs to the scientific field of plant biotechnology. The invention includes the steps of: 1) downloading the soybean whole genome sequence and acquiring MLO type gene; 2) identifying the MLO genotype; 3) identifying MLO gene phylogenetic relationship; and 4) comparing MLO-type powdery mildew gene. The invention effectively shortens the gene mining cycle for soybean powdery mildew, and is conducive to the rapid identification of anti-powdery mildew gene; corresponding coseparation functional markers (SNP, scar, etc.) are developed through the identified candidate powdery mildew genes, and the identified candidate powdery mildew genes can also be used for fast molecular marker assisted selection of anti-powdery mildew genes and have high accuracy; and combined with other resistant gene molecular markers, multi-resistance breeding material can be created, so as to shorten the breeding period and improve breeding efficiency. Therefore, the invention lays foundation for the explanation of powdery mildew resistant molecular mechanism of soybean.

Relating to plant comparative genomics, genetics, bioinformatics and other disciplinary knowledge, the invention belongs to the scientific field of plant biotechnology, and discloses rapid identification of phaseolus vulgaris mildew resistance locus o gene. The main steps include: 1) download of phaseolus vulgaris whole genome sequence and acquisition of MLO type gene; 2) identification of MLO type gene; 3) MLO type gene phylogenetic relationship; and 4) comparison of MLO type powdery mildew. The invention effectively shortens the mining cycle of phaseolus vulgaris powdery mildew gene, and is conducive to rapid identification of powdery mildew gene. Corresponding coseparation functional markers (SNP, SCAR and the like) are developed through identified candidate powdery mildew genes, which also can be used for molecular marker-assisted selection of mildew resistance locus o gene and have high accuracy. Multi-resistance breeding materials can be created by combining other resistant gene molecular markers, the breeding period can be shortened, and the breeding efficiency is improved, thus laying the foundation for elaborating the phaseolus vulgaris mildew resistance molecular mechanism.

The invention provides a rapid identification method of powdery mildew-resistant genes of cabbage, relating to subject knowledge such as comparative genomics of plants, genetics and bioinformatics and belonging to the field of plant biologic technical science. The rapid identification method mainly comprises the following steps: (1) downloading a whole genome sequence of the cabbage, and collecting MLO type genes; (2) identifying the MLO type genes; (3) establishing phylogenetic relationship of the MLO type genes; and (4) comparing MLO type powdery mildew genes. According to the rapid identification method, the mining period of powdery mildew genes of the cabbage is effectively shortened, and the rapid identification of the powdery mildew genes is benefited; by developing corresponding coseparation functional markers (SNP, SCAP and the like) by virtue of identified candidate powdery mildew genes, the molecular marker-assisted selection of the powdery mildew-resistant genes can be rapidly carried out, and the accuracy is high; by combining molecular markers of other disease-resistant genes, multi-resistance breeding materials can be created, the breeding age limit can be shortened, and the breeding efficiency can be improved; a foundation is laid for the exposition of a powdery mildew-resistant molecular mechanism of the cabbage.