55 results about "Enzyme structure" patented technology

The invention relates to a method for preparing dicarboxylic acid, in particular to a method for preparing adipic acid by biomimetic catalyzing oxygen oxidation of cyclohexane. The present invention selects metallophthalocyanines, mononuclear metalloporphyrins or μ-oxygen-binuclear metalloporphyrins similar in structure to biological enzymes as catalysts, and the dosage thereof is 0.1 to 1‰ of the weight of cyclohexane. As a solvent, 0.5-3.5 MPa of oxygen is passed through, the reaction temperature is controlled to be 110-160° C., and the reaction time is 4-32 hours. In the invention, the amount of catalyst is small, the reaction temperature and pressure are low, and the oxidation depth is easy to control. The catalysts used have good catalytic properties for the reaction of oxygen oxidation of cyclohexane to prepare adipic acid. The catalyst can be recycled to reduce the preparation cost. The invention adopts a one-step method to prepare adipic acid, and the reaction operation is simple and easy.

The present invention relates to methods for modifying the genome of a Bacillus host cell by employing a Class II Cas9 enzyme with only one active nuclease domain, e.g. the S. pyogenes Cas9 nickase, together with a suitable guide RNA for each target sequence to generate a site-specific nick in at least one genome target sequence followed by the repair of the nick(s) via integration of one or moremodified modified donor part of the Bacillus host cell genome through classical double homologous recombination on each side of the nick(s).

A process for producing enzyme structures includes providing an emulsion of droplets of a first liquid phase dispersed in a second liquid phase. The one liquid phase is a hydrophilic phase, while the other liquid phase is a hydrophobic phase which is immiscible with the hydrophilic phase. Enzyme molecules are located at or within interfacial boundaries of the droplets and the second liquid phase. The enzyme molecules of the respective droplets are cross-linked so that individual enzyme structures, which are stable and in which the enzymes are immobilized with a majority of active sites of the enzymes being orientated either internally or externally, are formed from individual droplets.

A process for producing enzymes structures includes providing an emulsion of droplets of a first liquid phase dispersed in a second liquid phase. The one liquid phase is a hydrophilic phase, while the other liquid phase is a hydrophobic phase which is immiscible with the hydrophilic phase. Enzyme molecules are located at or within interfacial boundaries of the droplets and the second liquid phase. The enzyme molecules of the respective droplets are cross-linked so that individual enzyme structures, which are stable and in which the enzymes are immobilized with a majority of active sites of the enzymes being orientated either internally or externally, are formed from individual droplets.

The object of the invention is a Mini-Ill RNase with amino acid sequence comprising an acceptor part, and a transplantable a4 helix, and a transplantable a5b-a6 loop, which form structures of a4 helixand a5b-a6 loop, respectively, in the Mini-Ill RNase structure, wherein the fragments which form structures of a4 helix and a5b-a6 loop, respectively, correspond structurally to respective structuresof a4 helix and a5b-a6 loop formed by amino acid sequence fragments 46-52 and 85-98, respectively, of Mini-Ill RNase from Bacillus subtilis shown in SEQ ID NO: 1, wherein the said Mini- Ill RNase exhibits sequence specificity in dsRNA cleavage being dependent only on a ribonucleotide sequence of the substrate, and independent from an occurrence of secondary structures in the substrate's structure, and independent from a presence of other assisting proteins, and wherein the Mini-Ill RNase is not the Mini-Ill protein from Bacillus subtilis of SEQ ID NO: 1, nor SEQ ID NO: 1 with D94R mutation. The invention also relates to a method of obtaining a chimeric Mini-Ill RNase, a Mini-Ill RNase encoding construct, a cell with a Mini-Ill RNase encoding gene, use of Mini-Ill RNase for dsRNA cleavage,as well as a method of dsRNA cleavage depending only on a ribonucleotide sequence.