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Target for preventing and killing weed-crofton weed and application of target

A technology for the prevention and control of Erythrina japonica, which is applied in the fields of application, herbicides and algicides, special data processing applications, etc., and can solve the problems of inability to carry out normal physiological activities, inability to synthesize, and plant death.

Inactive Publication Date: 2013-11-27
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the catalytic activity of the IspF enzyme is inhibited, this step of the reaction cannot proceed, thereby blocking the synthesis of isoprene pyrophosphate and dimethylpropylene pyrophosphate through the MEP pathway, and plants cannot synthesize isoprene pyrophosphate and dimethylene pyrophosphate synthesized through the MEP pathway. Propylene pyrophosphate is a substance with important physiological functions as a raw material, which cannot carry out normal physiological activities, such as photosynthesis, etc., and eventually leads to plant death

Method used

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  • Target for preventing and killing weed-crofton weed and application of target
  • Target for preventing and killing weed-crofton weed and application of target
  • Target for preventing and killing weed-crofton weed and application of target

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, the cloning of Eupatorium adenophorum EaispF1 gene

[0025] The seeds of Eupatorium adenophorum were collected from Yunnan Province. Mix the flower soil: vermiculite in a ratio of 2:1, put it into a pot with a diameter of 25cm, sprinkle the seeds of Eupatorium adenophorum on the moistened cultivation soil, and spread a layer of 2mm thick on the seeds. On the soil surface, cover the flower pot with paper to prevent excessive evaporation of water. After 5-7 days, after the Eupatorium adenophorum seedlings grow out, remove the paper on the flower pot and cultivate them in the greenhouse for 8 weeks. Extract total RNA from seedlings, such as figure 2 shown.

[0026] The cDNA sequence of the EaispF1 gene of Eupatorium adenophorum was screened out by in situ hybridization technology, and a positive clone was obtained after identification by enzyme digestion, which was identified as a partial sequence of the IspF gene. In order to obtain the full-length Eaisp...

Embodiment 2

[0033] Example 2 Obtaining of EaispF1 Gene Overexpression Arabidopsis Strains

[0034] The EaispF1 gene cDNA cloned in Example 1 was constructed into the plant expression vector pCAMBIA1300 with a CaMV35S promoter. Through the Agrobacterium-mediated pollen tube transformation method, the EaispF1 gene was transferred into Arabidopsis plant cells, and four transgene overexpressed Arabidopsis lines were screened, and the PCR identification results were as follows Figure 5 shown.

Embodiment 3

[0035] Example 3 EaispF1 subcellular localization

[0036]The signal peptide of EaispF1 indicates that the position of its protein expression is chloroplast. The subcellular localization of EaispF1 in transgenic Arabidopsis was determined by the expression of EaispF1-GFP in plants. The 5-day-old T2 generation transgenic seedlings were observed with a confocal laser scanning microscope, and it was found that the fluorescence signal of EaispF1-GFP overlapped with the red fluorescence signal of chlorophyll in the chloroplast of plant cells. Such as Image 6 , indicating that EaispF1 is located in the chloroplast in the cell.

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Abstract

The invention discloses a target for preventing and killing weed-crofton weed by using key enzyme (2-methyl-D-erythritol-2, 4-cyclic pyrophosphate cyclase, EaispF1) in a biosynthetic pathway of 2-methyl-D-erythritol-4-phosphoric acid and an application of the target. The target is protein of the following (a) or (b): (a) protein which consists of an amino acid sequence as shown in a sequence 2 in a sequence table; (b) protein which is formed by substituting and / or missing and / or adding one or more amino acids into the amino acid sequence as shown in the sequence 2 in the sequence table, associated with the target and derived from (a). The invention also relates to active compounds A and B designed based on EaispF1 enzyme structure of the crofton weed, and a synthetic method of the active compounds A and B.

Description

technical field [0001] The present invention relates to the key enzyme (2-methyl-D-erythritol-2,4-ring Pyrophosphate cyclase) as a target for controlling weeds-Eupatorium adenophorum and its application. Background technique [0002] Eupatorium adenophorum (Eupatorium adenophorum Spreng) belongs to the genus Eupatorium adenophorum in Asteraceae. It uses seeds for sexual reproduction, and also uses roots or stems for asexual reproduction, relying on powerful rhizomes to rapidly expand and spread. Eupatorium adenophorum has strong environmental adaptability, strong stress resistance and strong light adaptability. According to the survey, Eupatorium adenophorum is one of the most serious vicious invasive species in my country at present, ranking first in the "List of China's First Batch of Invasive Alien Species" issued by the National Environmental Protection Agency and the National Academy of Sciences. Eupatorium adenophorum has been colonized in Yunnan, Guizhou, Sichuan, G...

Claims

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Application Information

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IPC IPC(8): A01N43/54A01N43/40A01P13/02C07D213/74C07D239/47G06F19/00
Inventor 侯玉霞张晟瑞周思泓王平
Owner CHINA AGRI UNIV
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