146 results about "Subcellular localization" patented technology

The invention discloses a pennisetum purpureum gene PpbHLH148L and application thereof in improving the cold resistance of plants, and relates to the technical field of gene engineering. The nucleotide sequence of the gene is as shown in SEQ ID NO.5. After the gene is subjected to subcellular localization and expression condition analysis after cold stress, the gene is related to low-temperature tolerance. An overexpression vector of the gene is constructed, Agrobacterium tumefaciens is transferred, then Arabidopsis thaliana infection is carried out, cold tolerance determination is carried out on overexpressed Arabidopsis thaliana, phenotypic difference and physiological index difference between an overexpressed strain after cold treatment and a wild type are observed, and it is found that the gene can significantly improve the low-temperature stress tolerance of Arabidopsis thaliana. And in the low-temperature treatment of the pennisetum alopecuroides transgenic callus, the transgenic callus also shows higher tolerance than the wild callus at low temperature. The pennisetum purpureum gene PpbHLH148L can be used for low-temperature tolerance modification of plants, and has an application prospect in creation of low-temperature-resistant plant materials.

The invention belongs to the technical field of molecular biology, and particularly relates to a CeNF-YA3 gene, an expression vector and application of the CeNF-YA3 gene in vegetable fat regulation and control. The nucleotide sequence of the CeNF-YA3 gene is as shown in SEQ ID NO.1. After the CeNF-YA3 gene is over-expressed, the grease content of arabidopsis seeds and leaves can be remarkably increased; in addition, the invention also provides a series of vectors containing the CeNF-YA3 gene for subcellular localization, yeast hybridization and plant overexpression for the first time, and proves that the CeNF-YA3 protein has a transcriptional activation function, is localized in a cell nucleus and accords with the basic characteristics of transcription factors for the first time. Therefore, the technical scheme of the invention provides scientific guidance for the application of the CeNF-YA3 gene and the protein thereof.

The invention discloses application of an apple MdMYS1 gene in regulating and controlling the wax content of plant fruits and leaves, and belongs to the technical field of plant genetic engineering. According to the invention, the MdMYS1 gene with high expression quantity in apple varieties with more waxiness is separated, and the nucleotide sequence of the MdMYS1 gene is shown as SEQ ID NO. 1. Through subcellular localization, the transcription factor expressed by the MdMYS1 gene is found to be localized on a cell nucleus. Experimental results show that overexpression of the MdMYS1 gene can significantly increase the wax content of the apple fruits and leaves by promoting biosynthesis of wax, which indicates that the MdMYS1 gene plays a key role in regulation and control of the wax content of the apple fruits and leaves. The invention provides an efficient and rapid way for apple breeding, provides a gene material for improving apple quality, and has a wide application prospect in improving economic benefits and ecological benefits of apple planting.

The invention belongs to the technical field of molecular biology, and particularly relates to a CeNF-YA1 gene, an expression vector and application of the CeNF-YA1 gene in vegetable fat regulation and control. The invention provides the CeNF-YA1 gene for the first time, the nucleotide sequence of the CeNF-YA1 gene is as shown in SEQ ID NO.1, the full length of a coding region of the CeNF-YA1 gene is 792 bp, a series of carriers containing the CeNF-YA1 gene, such as subcellular localization, yeast hybridization and plant overexpression, are constructed for the first time, and meanwhile, the CeNF-YA1 protein is proved to have a transcription activation function, is localized in a cell nucleus and conforms to the basic characteristics of transcription factors. It is found for the first time that overexpression of the CeNF-YA1 gene can significantly improve the grease content of arabidopsis seeds and leaves; therefore, the technical scheme provided by the invention can be used for improving the oil content of the plant seeds and the nutritional tissues, and shows the application prospect in improving the oil content of the seeds and the nutritional tissues.

The invention discloses a long non-coding RNA subcellular localization prediction method and system, and belongs to the technical field of bioinformatics. The prediction method comprises the following steps: step 1, constructing a data set; 2, feature coding is carried out, and the sequence and secondary structure information of the lncRNA are extracted at the same time; 3, processing different types of features by adopting a pre-designed multi-channel model; and step 4, after the complete representation from the plurality of channels is obtained, inputting the feature vector output by each channel into a hierarchical adaptive fusion module for feature fusion, and generating a prediction result. The invention also discloses a long non-coding RNA subcellular localization prediction system. According to the method, a multi-channel model based on deep learning is constructed, the sequence and secondary structure information of the lncRNA are extracted at the same time in the feature coding stage, different channels are designed to process different types of features, and multi-label prediction of the lncRNA on seven subcellular localization is achieved.

The invention discloses a protein complex identification method based on knowledge enhancement graph comparative learning, and belongs to the technical field of bioinformatics. Edge trimming is performed by using a gene expression profile and subcellular localization data, so that a knowledge enhancement PPI network is constructed; and incorporating the molecular function and the biological process as network attributes as attributes for enhancing the network. Graph structure enhancement is applied to knowledge enhanced PPI attribute networks, and multiple perturbation views are generated with diverse random perturbation policies including edge addition, edge discarding, and feature masking to help mitigate data sparsity. A perturbation view generated by graph structure enhancement is coded through a randomized graph convolutional network layer, so that multi-scale features are extracted, key PPI interaction is reserved, and meanwhile prediction performance is improved. And finally, the original PPI network is weighted again, and the protein complex is identified by adopting a core-affiliated strategy.

The invention relates to the technical field of biomedicine detection reagent manufacturing and reproductive medicine, in particular to an embryonic development potential sperm key protein kit and a use method thereof. The method is characterized in that an antibody combination capable of specifically recognizing a group of key proteins related to embryonic development potential in human sperms is prepared, and the antibodies, a fluorescence labeled secondary antibody, a cell nucleus dye, a concentrated washing liquid, a mounting medium and a pre-coated control porous glass slide are assembled into the special detection kit. The kit can be combined with a multi-mode imaging system (such as a fluorescence / confocal microscope and a common optical microscope) and image analysis software to realize the traditional analysis of sperm morphology and movement ability, and can perform synchronous and accurate qualitative and quantitative analysis with the expression level and subcellular localization of the key protein. The invention provides an integrated molecular detection solution, and provides a powerful tool for sperm quality screening and embryonic development potential prediction in an assisted reproduction technology.

The invention discloses a cherry sugar transporter gene CpSWEET10 and application thereof, and belongs to the technical field of cherry gene engineering. A novel sugar transporter gene CpSWEET10 is obtained through cloning, the nucleotide sequence of the novel sugar transporter gene CpSWEET10 is shown as SEQ ID NO.1, and the coded amino acid sequence of the novel sugar transporter gene CpSWEET10 is shown as SEQ ID NO.2. The gene is highly expressed in cherry fruits, and the content of soluble solids and total sugar in mature fruits is remarkably positively correlated with the expression quantity of the soluble solids and the total sugar; through subcellular localization and sugar transport function defect type yeast mutant functional complementation experimental analysis, the protein coded by the CpSWEET10 gene is localized in a cytoplasmic membrane and has a sucrose transport function; the cherry sugar transporter gene CpSWEET10 disclosed by the invention can be applied to the improvement of the sugar degree character of the fruits, and a new gene resource is provided for molecular breeding for improving the high-sugar quality of the cherry fruits.

The invention relates to a key gene CbLTP63 for regulating and controlling salt stress response of catalpa bungei and application of the key gene CbLTP63, and belongs to the technical field of plant genetic engineering and biology. The nucleotide sequence of the CbLTP63 gene is as shown in Seq 1, and the coded amino acid sequence is as shown in Seq 2. The invention relates to cloning of a catalpa bungei salt stress response key gene CbLTP63. Identifying that the CbLTP63-GFP subcell is positioned on a cell membrane through an agrobacterium injection method; through qRT-PCR analysis and identification, the gene responds to salt stress on the transcriptional level and is down-regulated by H2O2, ABA and SA; through transgenosis identification, the gene is a catalpa bungei salt stress response negative regulation gene, and the salt tolerance of catalpa bungei calluses after gene silencing is obviously enhanced; the invention also relates to utilization of the gene in regulating stress response of catalpa bungei. The invention discloses a catalpa bungei salt stress response key gene CbLTP63, which has important application value in the fields of catalpa bungei gene engineering and clonal forestry.

The invention discloses an application of a cotton transcription factor GhMYB44A05 in improvement of cotton fiber quality. It is verified for the first time that the transcription factor GhMYB44A05 (GhiA05G026680) is an important gene in the cotton fiber development process, and the transcription factor is dominantly expressed in the cotton fiber elongation and thickening periods and the ovule development later period, so that the quality, such as the length and the strength, of the cotton fiber is affected. Subcellular localization analysis shows that the protein is localized in a cell nucleus and has general characteristics of transcription factors. Transgenic experiments prove that overexpression of the GhMYB44A05 can significantly increase the length and strength of the fibers, and the cultivation and development of high-fiber-quality cotton varieties are promoted.

The invention discloses a self-adaptive multi-channel fusion lncRNA subcellular localization prediction method based on a reinforcement learning agent, and relates to a long-chain non-coding RNA subcellular localization prediction method. The method aims at solving the problems that sample heterogeneity is ignored and RNA-protein interaction characteristics are ignored due to the fact that a fixed feature fusion strategy is adopted in an existing method. According to the method, firstly, multi-modal feature extraction is carried out, original feature vectors are obtained through splicing, the original feature vectors are projected to a reinforcement learning state, actions are obtained through an intelligent agent obtained through PPO training based on near-end strategy optimization, and the actions comprise feature selection mask codes, fusion weight guidance and dynamic hyper-parameter configuration. A personalized fusion strategy is obtained through action vectors generated by the reinforcement learning agent, the fusion prediction network fuses the features in the multi-modal feature set based on the personalized fusion strategy, and finally the positioning probability of the lncRNA at each subcell position is obtained.

The invention belongs to the technical field of genetic engineering, and relates to application of a transcription factor AcWRKY20 in resisting kiwifruit bacterial canker. Through gene cloning, phylogenetic analysis, subcellular localization, transgenic function verification and other technologies, it is proved for the first time that the transcription factor AcWRKY20 has the molecular characteristic of remarkably enhancing the disease resistance of kiwi fruits. According to the invention, the AcWRKY20 gene is respectively silenced and overexpressed and then is inoculated with Psa, and phenotypic observation shows that the gene is a disease-resistant gene and is suitable for canker-resistant breeding of kiwi fruit.

This invention discloses the application of the apple MdWRI1 gene in regulating the wax content of apple fruits and leaves, belonging to the field of plant genetic engineering technology. This invention isolates an MdWRI1 gene with high expression levels in apple varieties with high wax content, its nucleotide sequence of which is shown in SEQ ID NO.1. Subcellular localization revealed that the transcription factor expressing the MdWRI1 gene is located in the cell nucleus. Experimental results show that overexpression of the MdWRI1 gene can significantly increase the wax content of apple fruits and leaves by promoting wax biosynthesis, indicating that the MdWRI1 gene plays a key role in regulating the wax content of apple fruits and leaves. This invention provides an efficient and rapid approach for apple breeding, provides genetic material for improving apple quality, and has broad application prospects in improving the economic and ecological benefits of apple cultivation.

The invention relates to an NtWUN gene as well as an expression product, an expression vector and application of the NtWUN gene in regulation and control of plant glandular hair development, and aims to solve the technical problems that the development mechanism of different types of epidermal hairs in plants (such as tobacco) is unknown and effective molecular regulation and control measures and utilization ways are lacked at present. According to the invention, an NtWUN gene for negatively regulating epidermal hair development is identified, a gene editing vector and an overexpression vector are constructed, and a gene editing homozygous strain and an overexpression homozygous strain are obtained through screening; tissue expression, MeJA response mode analysis, subcellular localization, plant phenotypic characteristic identification and the like show that the NtWUN can negatively regulate the occurrence of epidermal hair and influence the change of the total density of the epidermal hair, and the NtWUN has important application value in regulating the development process of the plant epidermal hair.

The invention discloses application of a corn RTA9 gene in enhancing aphid resistance of plants in the technical field of plant genetic engineering, a CDS sequence of the corn RTA9 gene is shown as SEQ ID NO.1, an amino acid sequence for coding the corn RTA9 gene is shown as SEQ ID NO.2, subcellular localization shows that RTA9 is mainly localized in mitochondria, and it is known through research of the invention that the corn RTA9 gene can be used for improving aphid resistance of plants. The function deletion of the RTA9 gene weakens the capability of producing ROS (reactive oxygen species) in response to aphid infection of the corn, so that the aphid resistance of the corn is reduced. The content of ROS (reactive oxygen species) in cells can be remarkably increased by overexpressing RTA9 in corn, meanwhile, the aphid resistance of corn is improved, and a theoretical basis and gene resources are provided for developing aphid-resistant crop strains.

The invention discloses a cell wall associated kinase gene VvWAKL8 for regulating and controlling texture formation of grape fruits and application thereof, the cell wall associated kinase gene VvWAKL8 is cloned from the grape fruits for the first time, the nucleotide sequence of the cell wall associated kinase gene VvWAKL8 is as shown in SEQ ID NO.1, and the coded amino acid sequence is as shown in SEQ ID NO.2. Research finds that the VvWAKL8 is specifically and highly expressed in the hard-pulp grape variety, and subcellular localization analysis shows that the protein is localized in a cell membrane; instantaneous overexpression of VvWAKL8 in grape fruits can significantly improve fruit hardness and regulate pectin metabolism related gene expression, and verifies the core function of VvWAKL8 in fruit texture formation. The research reveals that the VvWAKL8 is a key gene for regulating and controlling the grape fruit texture for the first time, a brand new gene resource is provided for molecular marker-assisted breeding of a new storage-resistant grape variety, and an important foundation is laid for deeply analyzing a cell wall signal to regulate and control a molecular mechanism of fruit development.

The application discloses application of an apple MdMYS1 gene in regulation of wax content of plant fruits and leaves and belongs to the technical field of plant genetic engineering. The application separates a MdMYS1 gene with high expression in an apple variety with high wax content, and the nucleotide sequence of the MdMYS1 gene is shown as SEQ ID NO. 1. Subcellular localization shows that a transcription factor expressed by the MdMYS1 gene is located on a cell nucleus. Experimental results show that overexpression of the MdMYS1 gene can significantly improve the wax content of apple fruits and leaves by promoting biosynthesis of the wax, and the MdMYS1 gene plays a key role in regulation of the wax content of apple fruits and leaves. The application provides an efficient and rapid way for apple breeding, provides gene materials for improvement of apple quality, and has wide application prospects in improvement of economic and ecological benefits of apple planting.

The invention discloses a functionalized targeting fluorescent nanoprobe and application thereof in protein mutation analysis, and relates to the technical field of biology, the fluorescent nanoprobe comprises a targeting molecule-PEG hydrophilic bridging molecule and a matrix; the preparation method comprises the following steps: adding nano-seeds into fluorescent molecules to prepare fluorescent nano-particles, and stirring and mixing the fluorescent nano-particles with targeting molecules-PEG hydrophilic bridging molecules; the method is combined with deep learning to be applied to protein mutation analysis. The multifunctional integrated protein mutation receptor targeted fluorescent nanoprobe provided by the invention can accurately mark the position of a mutant protein at a single cell level, and can realize in-situ, real-time and dynamic living cell imaging of a tumor cell endogenous molecular mutant protein; and qualitative and quantitative analysis of tumor cell protein mutation can be realized through subcellular localization of the targeted fluorescent nanoprobe.

The invention discloses a method for detecting subcellular localization of a functional gene by using a tea tree leaf, a target gene is expressed in the tea tree leaf through an agrobacterium transformation method, the method has the advantages of short period, rapidness, high conversion rate, low cost, easiness in operation and the like, and the localization condition of the target gene in the tea tree leaf can be more intuitively and accurately observed; and important technical support is provided for tea tree gene function research. Compared with mode plants such as tobacco and arabidopsis thaliana, the novel subcellular localization experimental material has the advantages that the tea tree leaves are used for observing subcellular localization, and a more intuitive and more accurate mode is provided for gene function research in tea trees. In addition, the method is short in conversion period, fluorescence signals can be observed 36-48 hours after conversion, and the method is fast and efficient.

The invention discloses application of a SbMYB113-like gene to improvement of low-temperature-resistant breeding of scutellaria baicalensis, belongs to the technical field of plant biology, is used for scutellaria baicalensis, and is characterized in that the nucleotide sequence of the SbMYB113-like gene is shown as SEQ ID NO: 1, by reducing overexpression of the SbMYB113-like gene in the scutellaria baicalensis, the anti-oxidation defense system of the scutellaria baicalensis is improved, the stability of the cell structure and function is protected, and the stability of the low-temperature-resistant breeding of the scutellaria baicalensis is improved. The tolerance of the scutellaria baicalensis to low-temperature stress is improved. The invention aims at researching subcellular localization of SbMYB113-like protein, analyzing tissue specific expression and a low-temperature stress response expression mode of the SbMYB113-like gene in combination with real-time fluorescent quantitative PCR (Polymerase Chain Reaction), and verifying the cold resistance function of SbMYB113-like through genetic transformation, so that the SbMYB113-like gene has an application value in low-temperature scutellaria baicalensis breeding.

The present invention relates to the gene cloning and functional application of the salt-tolerant gene Hg52609 in the roots of Halogeton glomeratus, belonging to the field of plant bioengineering technology. The salt-tolerant gene Hg52609 was cloned from the roots of Halogeton glomeratus for the first time. By cloning the salt-tolerant related gene Hg52609 in the roots of Halogeton glomeratus, a subcellular localization expression vector was constructed, and gene localization was carried out through transient expression in tobacco; an overexpression vector was constructed, and Arabidopsis thaliana was stably genetically transformed for salt tolerance identification; a yeast heterologous expression vector was constructed to further verify the function of the Hg52609 gene. It was found that the Hg52609 gene is involved in the efflux of Na+ but does not mediate the absorption of K+ under high concentrations of Na+. The present invention provides the cloning and functional application of the salt-tolerant gene Hg52609 in the roots of Halogeton glomeratus, which will be a good material for exploring plant salt-tolerant genes and studying the salt-tolerant regulation mechanism, and has important application value for improving the stress resistance of crops and cultivating salt-tolerant and alkali-tolerant crops.

The invention discloses a drug delivery system containing loaded adriamycin as well as a preparation method and application of the drug delivery system. The drug delivery system comprises poly (beta-amino ester) coupled with adriamycin, wherein the surface of the poly (beta-amino ester) is coated with dextran sulfate, and the poly (beta-amino ester) takes a fourth-generation lysine dendritic molecule Lys-G4 as a core. The drug delivery system has a pH-dependent drug release characteristic, has remarkable toxicity to C6 and U87MG glioma cells, has the capabilities of inducing apoptosis, inhibiting migration and targeting subcellular localization, and can effectively penetrate through a blood-brain barrier model and 3D tumor spheres. The delivery system is compounded with PLA-PEG-PLA thermosensitive hydrogel, and the thermosensitive hydrogel with a drug storage function is successfully constructed. Good tumor inhibition effect and biocompatibility are shown in nude mouse subcutaneous and in-situ glioma models. The invention not only provides a new strategy for overcoming the blood brain barrier, but also can effectively reduce the systemic toxicity, and opens up a new research thought and method for glioma treatment.

This invention discloses a Panax notoginseng ethylene response factor gene. PnERF120 Its nucleotide sequence is shown in SEQ ID NO:1, and the encoded amino acid sequence is shown in SEQ ID NO:2. PnERF120 The gene originates from Panax notoginseng, and the encoded protein sequence contains a typical conserved domain of the ERF transcription factor. This invention demonstrates, through gene cloning, bioinformatics analysis, expression pattern analysis, subcellular localization, overexpression, and other techniques and methods, that... PnERF120 This invention can be used to improve the resistance of Panax notoginseng to Panax notoginseng A virus, and also to improve the resistance of tobacco to tobacco mosaic virus. This invention provides new functional genes and application pathways for antiviral breeding and disease-resistant materials of Panax notoginseng and other plants.

The invention discloses an exocytosis protein subcellular localization method for mannitol-induced plasm-wall separation, and relates to the technical field of cytobiology, the method comprises the following specific steps: constructing a fusion expression vector: connecting a plant target exocytosis protein gene with a fluorescent protein gene through a gene cloning technology to obtain a fusion expression vector; inserting into a plant expression vector to obtain a recombinant expression vector containing the target extracellular secretory protein-fluorescent protein fusion gene; performing bacterial colony PCR identification and sequencing verification on the recombinant expression vector; by adopting an agrobacterium tumefaciens-mediated plant leaf instantaneous transformation technology, the fusion gene is rapidly expressed in plant cells, subsequent experiments can be carried out after culture is carried out for 2-3 days, the experiment period is greatly shortened, meanwhile, leaf cells are induced to be subjected to plasmon-wall separation through a gradient mannitol solution, positive cell screening and identification are not needed, and the application prospect is wide. And positioning can be realized by directly observing fluorescence signal distribution by using a laser confocal microscope.

The application belongs to the field of subcellular localization prediction, and provides an lncRNA subcellular localization prediction method and system for unbalanced data, in order to fully utilize the sequence information of lncRNA, physical and chemical mode features and distributed representation features of nucleic acids are extracted, and two basic classifiers including a convolutional neural network and a gated recurrent unit are integrated, a subcellular classification result is obtained through adaptive weighted averaging of the two models, and subcellular localization is carried out based on the classification result, so that the precision of subcellular localization prediction on an unbalanced data set can be improved. In order to solve the problem that the model performs poorly on the unbalanced data set, a label distribution margin perception loss function is used in the training process. Compared with traditional machine learning models and existing predictors, the method has stronger tolerance to class imbalance and robustness.

The present invention discloses an AfGF14i gene of Aechmea fasciata, a cloning method, an expression vector and applications thereof, belonging to the field of biotechnology. Specifically, total RNA of Aechmea fasciata is extracted, reverse transcribed into cDNA, and taking this as a template, specific primers are designed, and the full-length CDS of the AfGF14i gene is obtained by PCR method, ligated with a vector, and transformed into Escherichia coli Trans-T1 competent cells, and positive clones are picked to obtain the AfGF14i gene of Aechmea fasciata. The full-length CDS of this gene is 729 bp, encoding a protein containing 243 amino acid residues. The present invention uses transient expression and fluorescence microscopy to detect that the subcellular localization of the protein encoded by the AfGF14i gene is the cell membrane. Through transgenic technology, it is found that the overexpression of the AfGF14i gene promotes the early flowering of Arabidopsis thaliana. The acquisition of the AfGF14i gene lays a foundation for studying the flowering mechanism of Aechmea fasciata, provides a theoretical reference for overcoming the high-temperature obstacle of ethylene-induced flowering of pineapple in production, and has important theoretical and practical significance.

The invention relates to the field of plant genetic engineering, in particular to a rosa chinensis high-temperature-resistant gene RcbHLH59 and application thereof.The rosa chinensis high-temperature-resistant gene RcbHLH59 is identified and cloned to obtain the RcbHLH59 gene, and a CDS sequence and a coded amino acid sequence of the RcbHLH59 gene are obtained; secondly, constructing an overexpression vector and a VIGS silencing vector of the gene, performing agrobacterium-mediated transformation on the Chinese rose, verifying that the gene is a nuclear transcription factor in combination with subcellular localization, and verifying that leaves of the Chinese rose overexpressing the gene are dark green and upright, leaves of the Chinese rose silencing the gene are more serious in yellowing and wilting through a high-temperature stress experiment; according to the rosa chinensis high-temperature-resistant gene RcbHLH59 and the application thereof, eight related genes capable of regulating eight high-temperature response metabolic pathways can be regulated, rosa chinensis heat-resistant gene resources can be enriched, a key target is provided for rosa chinensis high-temperature-resistant molecular breeding, the growth vigor and ornamental value of rosa chinensis at high temperature are effectively improved, and popularization and application of the rosa chinensis in landscaping are promoted.

The invention relates to the technical field of monoclonal antibodies, in particular to a monoclonal antibody targeting EcSSP1 protein and application of the monoclonal antibody in detection of microsporidia. The amino acid sequence of a heavy chain variable region of the monoclonal antibody provided by the invention is as shown in SEQ ID NO.9, and the amino acid sequence of a light chain variable region of the monoclonal antibody is as shown in SEQ ID NO.10. The monoclonal antibody provided by the invention is obtained by screening by taking the recombinant protein EcSSP1 as an immunogen, has relatively high specificity, can be positioned on the surface of microsporidia, has relatively high detection accuracy and sensitivity, can be used for detecting in-vivo microsporidia in the early stage of infection, and can be used for detecting microsporidia in vivo. And if the antibody is positive, the current infection of the microsporidia can be confirmed, the operation is simple and convenient, the subcellular localization research of the microsporidia protein can be carried out by utilizing a multicolor fluorescence labeling method, and the application prospect is relatively good.

The application discloses a preparation method and application of a hollow manganese dioxide nanocarrier coated with a human umbilical cord mesenchymal stem cell membrane and a transmembrane peptide TAT, and is characterized in that: hollow mesoporous manganese dioxide is synthesized through a selective etching technology, and a layer of human umbilical cord mesenchymal stem cell membrane is coated outside the manganese dioxide through a repeated extrusion method. Since the cell membrane has special tumor homing characteristics, and lacks major histocompatibility complex-II and only expresses major histocompatibility complex-I in a trace amount, the key scientific problems and difficulties such as low biological safety, strong immunogenicity and easy elimination by the body of artificially synthesized nanomaterials can be effectively solved. Finally, the cell membrane surface is modified with the transmembrane peptide TAT through a phospholipid insertion method, precise subcellular localization-nucleus targeting is realized, and tumor cells can be more effectively killed.

The application belongs to the field of molecular biological technology, and particularly relates to CeABI3B a gene, an expression vector and application thereof in plant oil regulation. CeABI3B The application provides a gene for the first time, a nucleotide sequence of which is shown as SEQ ID NO. 1, and a coding region of which has a full length of 1707 bp. CeABI3B A series of vectors containing the gene are constructed for the first time, including subcellular localization, yeast hybridization and plant overexpression, and it is proved that the CeABI3B protein has a transcription activation function and is located in the cell nucleus, which is in line with the basic characteristics of transcription factors. CeABI3B Not only is it found that transient overexpression of the gene can significantly improve the oil content of tobacco leaves, but also it is proved that overexpression of the gene can significantly improve the oil content of transgenic Arabidopsis thaliana seeds and leaves. CeABI3B Therefore, the cloned and identified gene can be used to improve the oil content of plant seeds and nutrient tissues, and has a wide application prospect.