59 results about "Subcellular localization" patented technology

The invention discloses a cherry sugar transporter gene CpSWEET10 and application thereof, and belongs to the technical field of cherry gene engineering. A novel sugar transporter gene CpSWEET10 is obtained through cloning, the nucleotide sequence of the novel sugar transporter gene CpSWEET10 is shown as SEQ ID NO.1, and the coded amino acid sequence of the novel sugar transporter gene CpSWEET10 is shown as SEQ ID NO.2. The gene is highly expressed in cherry fruits, and the content of soluble solids and total sugar in mature fruits is remarkably positively correlated with the expression quantity of the soluble solids and the total sugar; through subcellular localization and sugar transport function defect type yeast mutant functional complementation experimental analysis, the protein coded by the CpSWEET10 gene is localized in a cytoplasmic membrane and has a sucrose transport function; the cherry sugar transporter gene CpSWEET10 disclosed by the invention can be applied to the improvement of the sugar degree character of the fruits, and a new gene resource is provided for molecular breeding for improving the high-sugar quality of the cherry fruits.

This invention discloses the application of the apple MdWRI1 gene in regulating the wax content of apple fruits and leaves, belonging to the field of plant genetic engineering technology. This invention isolates an MdWRI1 gene with high expression levels in apple varieties with high wax content, its nucleotide sequence of which is shown in SEQ ID NO.1. Subcellular localization revealed that the transcription factor expressing the MdWRI1 gene is located in the cell nucleus. Experimental results show that overexpression of the MdWRI1 gene can significantly increase the wax content of apple fruits and leaves by promoting wax biosynthesis, indicating that the MdWRI1 gene plays a key role in regulating the wax content of apple fruits and leaves. This invention provides an efficient and rapid approach for apple breeding, provides genetic material for improving apple quality, and has broad application prospects in improving the economic and ecological benefits of apple cultivation.

The invention discloses a cell wall associated kinase gene VvWAKL8 for regulating and controlling texture formation of grape fruits and application thereof, the cell wall associated kinase gene VvWAKL8 is cloned from the grape fruits for the first time, the nucleotide sequence of the cell wall associated kinase gene VvWAKL8 is as shown in SEQ ID NO.1, and the coded amino acid sequence is as shown in SEQ ID NO.2. Research finds that the VvWAKL8 is specifically and highly expressed in the hard-pulp grape variety, and subcellular localization analysis shows that the protein is localized in a cell membrane; instantaneous overexpression of VvWAKL8 in grape fruits can significantly improve fruit hardness and regulate pectin metabolism related gene expression, and verifies the core function of VvWAKL8 in fruit texture formation. The research reveals that the VvWAKL8 is a key gene for regulating and controlling the grape fruit texture for the first time, a brand new gene resource is provided for molecular marker-assisted breeding of a new storage-resistant grape variety, and an important foundation is laid for deeply analyzing a cell wall signal to regulate and control a molecular mechanism of fruit development.

The application discloses application of an apple MdMYS1 gene in regulation of wax content of plant fruits and leaves and belongs to the technical field of plant genetic engineering. The application separates a MdMYS1 gene with high expression in an apple variety with high wax content, and the nucleotide sequence of the MdMYS1 gene is shown as SEQ ID NO. 1. Subcellular localization shows that a transcription factor expressed by the MdMYS1 gene is located on a cell nucleus. Experimental results show that overexpression of the MdMYS1 gene can significantly improve the wax content of apple fruits and leaves by promoting biosynthesis of the wax, and the MdMYS1 gene plays a key role in regulation of the wax content of apple fruits and leaves. The application provides an efficient and rapid way for apple breeding, provides gene materials for improvement of apple quality, and has wide application prospects in improvement of economic and ecological benefits of apple planting.

The invention discloses a functionalized targeting fluorescent nanoprobe and application thereof in protein mutation analysis, and relates to the technical field of biology, the fluorescent nanoprobe comprises a targeting molecule-PEG hydrophilic bridging molecule and a matrix; the preparation method comprises the following steps: adding nano-seeds into fluorescent molecules to prepare fluorescent nano-particles, and stirring and mixing the fluorescent nano-particles with targeting molecules-PEG hydrophilic bridging molecules; the method is combined with deep learning to be applied to protein mutation analysis. The multifunctional integrated protein mutation receptor targeted fluorescent nanoprobe provided by the invention can accurately mark the position of a mutant protein at a single cell level, and can realize in-situ, real-time and dynamic living cell imaging of a tumor cell endogenous molecular mutant protein; and qualitative and quantitative analysis of tumor cell protein mutation can be realized through subcellular localization of the targeted fluorescent nanoprobe.

The invention discloses a method for detecting subcellular localization of a functional gene by using a tea tree leaf, a target gene is expressed in the tea tree leaf through an agrobacterium transformation method, the method has the advantages of short period, rapidness, high conversion rate, low cost, easiness in operation and the like, and the localization condition of the target gene in the tea tree leaf can be more intuitively and accurately observed; and important technical support is provided for tea tree gene function research. Compared with mode plants such as tobacco and arabidopsis thaliana, the novel subcellular localization experimental material has the advantages that the tea tree leaves are used for observing subcellular localization, and a more intuitive and more accurate mode is provided for gene function research in tea trees. In addition, the method is short in conversion period, fluorescence signals can be observed 36-48 hours after conversion, and the method is fast and efficient.

The invention discloses application of a SbMYB113-like gene to improvement of low-temperature-resistant breeding of scutellaria baicalensis, belongs to the technical field of plant biology, is used for scutellaria baicalensis, and is characterized in that the nucleotide sequence of the SbMYB113-like gene is shown as SEQ ID NO: 1, by reducing overexpression of the SbMYB113-like gene in the scutellaria baicalensis, the anti-oxidation defense system of the scutellaria baicalensis is improved, the stability of the cell structure and function is protected, and the stability of the low-temperature-resistant breeding of the scutellaria baicalensis is improved. The tolerance of the scutellaria baicalensis to low-temperature stress is improved. The invention aims at researching subcellular localization of SbMYB113-like protein, analyzing tissue specific expression and a low-temperature stress response expression mode of the SbMYB113-like gene in combination with real-time fluorescent quantitative PCR (Polymerase Chain Reaction), and verifying the cold resistance function of SbMYB113-like through genetic transformation, so that the SbMYB113-like gene has an application value in low-temperature scutellaria baicalensis breeding.

The invention discloses a drug delivery system containing loaded adriamycin as well as a preparation method and application of the drug delivery system. The drug delivery system comprises poly (beta-amino ester) coupled with adriamycin, wherein the surface of the poly (beta-amino ester) is coated with dextran sulfate, and the poly (beta-amino ester) takes a fourth-generation lysine dendritic molecule Lys-G4 as a core. The drug delivery system has a pH-dependent drug release characteristic, has remarkable toxicity to C6 and U87MG glioma cells, has the capabilities of inducing apoptosis, inhibiting migration and targeting subcellular localization, and can effectively penetrate through a blood-brain barrier model and 3D tumor spheres. The delivery system is compounded with PLA-PEG-PLA thermosensitive hydrogel, and the thermosensitive hydrogel with a drug storage function is successfully constructed. Good tumor inhibition effect and biocompatibility are shown in nude mouse subcutaneous and in-situ glioma models. The invention not only provides a new strategy for overcoming the blood brain barrier, but also can effectively reduce the systemic toxicity, and opens up a new research thought and method for glioma treatment.

This invention discloses a Panax notoginseng ethylene response factor gene. PnERF120 Its nucleotide sequence is shown in SEQ ID NO:1, and the encoded amino acid sequence is shown in SEQ ID NO:2. PnERF120 The gene originates from Panax notoginseng, and the encoded protein sequence contains a typical conserved domain of the ERF transcription factor. This invention demonstrates, through gene cloning, bioinformatics analysis, expression pattern analysis, subcellular localization, overexpression, and other techniques and methods, that... PnERF120 This invention can be used to improve the resistance of Panax notoginseng to Panax notoginseng A virus, and also to improve the resistance of tobacco to tobacco mosaic virus. This invention provides new functional genes and application pathways for antiviral breeding and disease-resistant materials of Panax notoginseng and other plants.

The invention relates to the field of plant genetic engineering, in particular to a rosa chinensis high-temperature-resistant gene RcbHLH59 and application thereof.The rosa chinensis high-temperature-resistant gene RcbHLH59 is identified and cloned to obtain the RcbHLH59 gene, and a CDS sequence and a coded amino acid sequence of the RcbHLH59 gene are obtained; secondly, constructing an overexpression vector and a VIGS silencing vector of the gene, performing agrobacterium-mediated transformation on the Chinese rose, verifying that the gene is a nuclear transcription factor in combination with subcellular localization, and verifying that leaves of the Chinese rose overexpressing the gene are dark green and upright, leaves of the Chinese rose silencing the gene are more serious in yellowing and wilting through a high-temperature stress experiment; according to the rosa chinensis high-temperature-resistant gene RcbHLH59 and the application thereof, eight related genes capable of regulating eight high-temperature response metabolic pathways can be regulated, rosa chinensis heat-resistant gene resources can be enriched, a key target is provided for rosa chinensis high-temperature-resistant molecular breeding, the growth vigor and ornamental value of rosa chinensis at high temperature are effectively improved, and popularization and application of the rosa chinensis in landscaping are promoted.

The application discloses a preparation method and application of a hollow manganese dioxide nanocarrier coated with a human umbilical cord mesenchymal stem cell membrane and a transmembrane peptide TAT, and is characterized in that: hollow mesoporous manganese dioxide is synthesized through a selective etching technology, and a layer of human umbilical cord mesenchymal stem cell membrane is coated outside the manganese dioxide through a repeated extrusion method. Since the cell membrane has special tumor homing characteristics, and lacks major histocompatibility complex-II and only expresses major histocompatibility complex-I in a trace amount, the key scientific problems and difficulties such as low biological safety, strong immunogenicity and easy elimination by the body of artificially synthesized nanomaterials can be effectively solved. Finally, the cell membrane surface is modified with the transmembrane peptide TAT through a phospholipid insertion method, precise subcellular localization-nucleus targeting is realized, and tumor cells can be more effectively killed.

The application belongs to the field of molecular biological technology, and particularly relates to CeABI3B a gene, an expression vector and application thereof in plant oil regulation. CeABI3B The application provides a gene for the first time, a nucleotide sequence of which is shown as SEQ ID NO. 1, and a coding region of which has a full length of 1707 bp. CeABI3B A series of vectors containing the gene are constructed for the first time, including subcellular localization, yeast hybridization and plant overexpression, and it is proved that the CeABI3B protein has a transcription activation function and is located in the cell nucleus, which is in line with the basic characteristics of transcription factors. CeABI3B Not only is it found that transient overexpression of the gene can significantly improve the oil content of tobacco leaves, but also it is proved that overexpression of the gene can significantly improve the oil content of transgenic Arabidopsis thaliana seeds and leaves. CeABI3B Therefore, the cloned and identified gene can be used to improve the oil content of plant seeds and nutrient tissues, and has a wide application prospect.

The application discloses application of TRIM11 protein in preparation of a medicine for treating spinocerebellar ataxia 51. The application adopts an SCA51 specific cell model, and identifies, through a large number of screening experiments, that the TRIM11 protein has the function of significantly removing mutant THAP11 protein and aggregates thereof. Experimental data show that the mutant protein removal efficiency of the TRIM11 treatment group is significantly better than that of the control group. The application verifies the removal effect of TRIM11 on the SCA51 pathological protein from the two dimensions of protein expression level and subcellular localization through Western blotting quantitative analysis and immunofluorescence co-localization technology. The three-dimensional reconstruction image clearly shows that the mutant protein aggregates are significantly reduced. The application first establishes a complete research system from target discovery to mechanism analysis, and provides a new intervention strategy for precise treatment of SCA51.

The present application belongs to the field of chemical synthesis and biotechnology, and particularly relates to a kind of sugar metabolism product molecular probes and preparation method thereof. Starting from fructose, a bio-orthogonal group is added to the 4-position hydroxyl group of the compound without affecting the biological activity of the sugar metabolism product. The present application provides a kind of sugar metabolism product probe and preparation method thereof, compared with the prior art, has the following beneficial effects: (1) the present application first synthesizes an endogenous sugar metabolism product molecular probe that can undergo click chemistry reaction, by adding a bio-orthogonal group to the 4-position hydroxyl group, the biological activity of the sugar metabolism product is maintained, which can be used for live cell imaging; (2) the probe of the present application has not been reported, and the synthesis method has less side reaction, mild reaction condition, good selectivity, can reflect the behavior of endogenous sugar metabolism product in cells, including direct reaction of sugar metabolism product in live cells Subcellular localization and tracing, is an important technical support for the study of live cell sugar metabolism.

The application discloses a kind of lncRNA subcellular localization prediction method based on lightGBM, comprising, first, the nucleotide of the front section multiple of known lncRNA sequence is intercepted as sequence sample one;Then by based on single strand multiclass position-specific three nucleotide bias and reverse complementary kmer, sequence sample one is respectively characterized coding, the combination of two kinds of feature coding is vector;Use lightGMB as learning algorithm;Using 5-fold cross validation optimization reverse complementary kmer and LightGBM's hyperparameter;Most intercept unknown lncRNA sequence front end multiple bases as sequence sample two, and its single strand multiclass position-specific three nucleotide bias and the combination of optimized reverse complementary kmer feature coding is input in trained lightGBM, will obtain its localization subcellular type, the patent of the application can be according to long-chain non-coding RNA sequence prediction in cytoplasm, nucleus, ribosome, cytosol, exosome five subcellular positions, the application realizes simply, and prediction precision is high.

The application discloses a transcription factor SrMYB1 for regulating synthesis of steviol glycoside, an expression protein thereof and application, and belongs to the technical field of plant genetic engineering. The transcription factor SrMYB1 has a nucleotide sequence as shown in SEQ ID NO. 1, and the amino acid sequence of the expression protein is as shown in SEQ ID NO. 2. Subcellular localization in tobacco epidermal cells shows that the protein coded by the gene is mainly located in the nucleus; yeast activity experiment shows that the protein has transcription activation activity. Yeast single hybridization experiment shows that SrMYB1 can be combined on the promoter of a key gene for synthesis of steviol glycoside SrUGT76G1 . Promoter activity and transgenic experiment both show that SrMYB1 significantly inhibits transcription of the key gene for synthesis of steviol glycoside SrUGT76G1 . Based on the characteristics, certain reference can be provided for quality regulation of steviol glycoside and cultivation of new steviol varieties.

The invention relates to a recombinant microbial strain for de novo synthesis of gardenin B and application of the recombinant microbial strain in production of polymethoxylated flavone compounds, and belongs to a cross technology of synthetic biology and organic synthetic chemistry. The invention designs and constructs a heterozygous gardenin B biosynthesis pathway, and obtains a microbial strain for synthesizing gardenin B from the beginning. By screening F8H and redox partner types and regulating and controlling subcellular localization, an F8H-redox partner combination with optimal space and type is determined, and an engineering strain of which the gardenin B yield is greatly improved is obtained. The efficient synthesis method of the polymethoxylated flavonoids is established by selecting recombinant microorganisms for synthesizing gardenin B from de novo to perform co-culture fermentation or chemical conversion of fermentation products.

The application discloses the field of plant genetic engineering technology and relates to application of a maize RTA9 gene in enhancing the ability of plants to resist aphids, a CDS sequence of the maize RTA9 gene is shown as SEQ ID NO.1, and an amino acid sequence of the RTA9 gene is shown as SEQ ID NO.2; subcellular localization shows that RTA9 is mainly located in mitochondria; researches show that the function loss of the RTA9 gene weakens the ability of maize to produce ROS in response to aphid infestation, thereby causing the ability of maize to resist aphids to decrease; overexpression of the RTA9 gene in maize can significantly increase the content of intracellular ROS and improve the ability of maize to resist aphids; and the application provides a theoretical basis and gene resources for development of crop strains resistant to aphids.

The present application discloses a protein marker for early pregnancy detection of mammals, and particularly relates to a hidden marker protein serum amyloid A of early pregnancy test paper. Protein sequencing used in the present application adopts liquid chromatography-mass spectrometry (LC-MS) technology, sample correlation is compared through Venn, sample correlation, PCA and PLS-DA analysis; protein function annotation is carried out through GO, KEGG, COG and Pfam, and subcellular localization prediction is carried out; difference protein, protein set and difference metabolite are calculated and counted; correlation analysis of proteome and transcriptome is carried out, difference protein of sheep serum in early pregnancy is obtained, and a key marker protein of early pregnancy of sheep is screened, which provides a basis for unknown early pregnancy detection of sheep.

The invention relates to the technical field of molecular biology, and particularly provides a suaeda heteroptera SsWRKY30 gene and application thereof, and the nucleotide sequence of the suaeda heteroptera SsWRKY30 gene is as shown in SEQ ID NO. 1. According to the invention, the SsWRKY30 gene is transferred into tobacco by means of molecular biology and biotechnology, so that a subcellular localization result of the SsWRKY30 gene is obtained; the SsWRKY30 gene is transferred into arabidopsis thaliana to obtain an overexpressed arabidopsis thaliana plant, and comparative analysis proves that the tolerance of the overexpressed arabidopsis thaliana to salt and drought stress is obviously improved.

The invention discloses an antibody specifically bound with arabidopsis thaliana blue light receptor cryptoflorin CRY1 and application thereof, and belongs to the technical field of biomedicine, the antibody has high specificity and high affinity when bound with arabidopsis thaliana CRY1 protein, and does not have cross reaction with arabidopsis thaliana CRY2, human CRY family protein and other species of photolyase / cryptoflorin, and the antibody can be used for detecting the photolyase / cryptoflorin of the arabidopsis thaliana blue light receptor cryptoflorin CRY1. The antibody can effectively solve the problems that an existing CRY1 detection method is poor in specificity, insufficient in sensitivity, unstable in result and the like, and a reliable tool is provided for expression dynamic tracking, subcellular localization and protein interaction network analysis of arabidopsis thaliana CRY1 protein.

The application discloses a plant core related gene and application thereof, and belongs to the technical field of plant genetic engineering and vegetable molecular breeding. RsERF013 Through complete genetic analysis, transcriptome and metabolome sequencing analysis, subcellular localization and transgenic strain construction, the relationship between the core related gene and the occurrence, development and RsERF013 metabolic mechanism of the radish core phenotype is clarified 。 The core related gene provided by the application RsERF013 can be widely applied to the fields of phenotype identification, breeding detection and model construction.

The present application relates to a single-cell protein subcellular localization model based on weakly supervised multi-instance learning, and relates to the technical field of biological information. The model comprises a cell feature extractor, an image branch, a cell branch and a class-aware adaptive pruning module. The cell feature extractor is used to encode single-cell images into single-cell feature vectors. An image-level classifier in the image branch is used to output an image-level protein subcellular localization prediction result according to the image-level feature representation. A cell-level classifier in the cell branch is used to output a single-cell-level protein subcellular localization prediction result according to the single-cell feature vector. The class-aware adaptive pruning module generates single-cell pseudo labels through several label sources. The model of the present application can solve the problems of cell label noise and class long-tail distribution under weak supervision, thereby realizing more accurate and stable single-cell localization prediction and providing a powerful tool for single-cell heterogeneity analysis.

This invention relates to the Bg.MEW_38304 gene regulating plant growth and flowering, its encoded protein, and its applications, belonging to the fields of plant molecular biotechnology and genetic engineering. This gene is derived from *Prunus glabra*, with a full-length CDS sequence of 378 bp. The encoded protein contains a conserved SBP domain, and its nucleotide sequence is shown in SEQ ID NO.1. It was cloned from the bracts of the 'Snow White' variety at the BR3 stage. Bioinformatics analysis, subcellular localization, virus-induced gene silencing, and heterologous overexpression experiments verified that the protein encoded by this gene is located in the cell nucleus and cell membrane. Silencing this gene leads to bract and stem / leaf abortion in *Prunus glabra*, while overexpression promotes growth and earlier flowering in *Arabidopsis thaliana*. This application identifies a key gene for *Prunus glabra* bract development, which can be used to promote plant growth and flowering, and has important application value in the molecular breeding of ornamental plants.

This invention discloses VvENO2 The application of genes in regulating plant fruit development belongs to the field of plant genetic engineering technology. Previous research in this invention found that hydrogen peroxide (H2O2) treatment significantly promotes grape fruit ripening. Transcriptome analysis of H2O2-treated grapes revealed… VvENO2 Gene expression levels decreased. To further clarify... VvENO2 The role of the gene in plant fruit development was investigated through subcellular localization experiments and promoter activity analysis, demonstrating its nucleus location and that its promoter can activate the GUS reporter protein. Furthermore, treatment with ABA and H2O2 enhanced its activity. In addition, transgenic Arabidopsis and transgenic grape were constructed, and phenotypic observations confirmed... VvENO2 Genes negatively regulate the ripening of plant fruits. This invention provides a new regulatory site for breeding early-maturing plant varieties.

The invention discloses a subcellular localization method suitable for floating moss, and belongs to the technical field of mutation or genetic engineering and DNA or RNA and vectors related to the genetic engineering. According to the method disclosed by the invention, the tender thallus of the floating moss is used as a material, and a related protein positioning gene is transferred into a floating moss species through a floating moss stable genetic transformation system. A series of subcellular localization genes are obtained through cloning in a floating moss genome for the first time, the genes can assist GFP fusion protein to enter different subcellular organelles, and a marker line (localization strain) is obtained through stable transformation of a floating moss material. The method can be used for verifying a subcellular localization result of a candidate floating moss target gene, and is a simple, convenient and stable floating moss subcellular localization method. The method has a wide application prospect.

The invention relates to the cross field of toxicology and immunology, in particular to an intervention method for relieving T-2 toxin induced cellular immune aging based on PER2 protein inhibition and application of the intervention method. The method comprises the following steps: S1, providing an exposed object containing immune cells in an in-vitro culture system; s2, firstly applying a PER2 inhibitor; s3, sequentially finishing S2 and T-2 treatment according to a preset initial administration time interval; and S4, characterizing the Hippo pathway MST1 / YAP / p-YAP in 4 hours and / or 12 hours after T-2 treatment, and obtaining p-YAP subcellular localization, thereby determining or adjusting the administration time course. According to the scheme, closed-loop standard operation and parameter setting are carried out according to'administration-detection-time history determination '.

The invention discloses a targeted modified fungal luciferase protein and application thereof in enhancing the bioluminescence intensity of fungi or plants. The invention aims to solve the problems of subcellular localization disorder and activity loss caused by deletion of natural membrane localization signals of fungal luciferase in heterologous systems such as plants and the like. The invention creatively provides an innovative positioning reconstruction strategy: removing a natural membrane positioning sequence at the N end of the Luz protein, and then carrying out double modification on the N end and the C end. The method specifically comprises the following steps: fusing endoplasmic reticulum targeting signal peptide at the N end to guide a target protein into an endoplasmic reticulum cavity; meanwhile, endoplasmic reticulum retention signal peptide is fused at the C end of the Luz protein, so that the Luz protein is stably retained in an endoplasmic reticulum, subcellular localization of the Luz protein is accurately reconstructed, Luz is ensured to be localized into cytoplasm, and the Luz protein is prevented from entering a secretion path, so that the expression level and activity of the Luz protein in a fungus or plant system are remarkably improved, and the bioluminescence intensity of fungi or plants is remarkably enhanced.

This invention belongs to the field of plant genetic engineering technology, specifically involving the BnARF22 gene and protein and their application in regulating the plant height of Brassica napus. The dwarfing gene BnARF22 was isolated from the dwarf mutant M176 of Brassica napus. Subcellular localization results showed that BnARF22 is located in the cell nucleus. This invention constructed a BnARF22 overexpression vector and transformed it into the wild-type Brassica napus variety Zhongshuang 11. Compared with the control Zhongshuang 11, the transgenic positive plants showed significantly reduced plant height, fewer branches, and a delayed flowering period of 3-5 days. This invention demonstrates that BnARF22 can control the plant height of rapeseed and has important utilization value for dwarfing breeding of rapeseed.

The present disclosure provides compounds comprising photocaged chemical moieties (e.g., tetrazines) and a click chemistry handle or a halotag ligand. The compounds described herein are capable of serving as "photo-click" adapters between recognition groups on the surfaces of cells and diverse chemical payloads. The present disclosure also provides compositions, systems, and kits comprising any of the compounds provided herein. Methods for optical and chemical tagging of cells using the compounds described herein are also provided by the present disclosure. Such methods may be used, for example, to monitor the expression and / or subcellular location of the one or more proteins of interest.