111 results about "Subcellular localization" patented technology

The invention belongs to the technical field of molecular biology, and particularly relates to a CeNF-YA3 gene, an expression vector and application of the CeNF-YA3 gene in vegetable fat regulation and control. The nucleotide sequence of the CeNF-YA3 gene is as shown in SEQ ID NO.1. After the CeNF-YA3 gene is over-expressed, the grease content of arabidopsis seeds and leaves can be remarkably increased; in addition, the invention also provides a series of vectors containing the CeNF-YA3 gene for subcellular localization, yeast hybridization and plant overexpression for the first time, and proves that the CeNF-YA3 protein has a transcriptional activation function, is localized in a cell nucleus and accords with the basic characteristics of transcription factors for the first time. Therefore, the technical scheme of the invention provides scientific guidance for the application of the CeNF-YA3 gene and the protein thereof.

The invention discloses application of an apple MdMYS1 gene in regulating and controlling the wax content of plant fruits and leaves, and belongs to the technical field of plant genetic engineering. According to the invention, the MdMYS1 gene with high expression quantity in apple varieties with more waxiness is separated, and the nucleotide sequence of the MdMYS1 gene is shown as SEQ ID NO. 1. Through subcellular localization, the transcription factor expressed by the MdMYS1 gene is found to be localized on a cell nucleus. Experimental results show that overexpression of the MdMYS1 gene can significantly increase the wax content of the apple fruits and leaves by promoting biosynthesis of wax, which indicates that the MdMYS1 gene plays a key role in regulation and control of the wax content of the apple fruits and leaves. The invention provides an efficient and rapid way for apple breeding, provides a gene material for improving apple quality, and has a wide application prospect in improving economic benefits and ecological benefits of apple planting.

The invention belongs to the technical field of molecular biology, and particularly relates to a CeNF-YA1 gene, an expression vector and application of the CeNF-YA1 gene in vegetable fat regulation and control. The invention provides the CeNF-YA1 gene for the first time, the nucleotide sequence of the CeNF-YA1 gene is as shown in SEQ ID NO.1, the full length of a coding region of the CeNF-YA1 gene is 792 bp, a series of carriers containing the CeNF-YA1 gene, such as subcellular localization, yeast hybridization and plant overexpression, are constructed for the first time, and meanwhile, the CeNF-YA1 protein is proved to have a transcription activation function, is localized in a cell nucleus and conforms to the basic characteristics of transcription factors. It is found for the first time that overexpression of the CeNF-YA1 gene can significantly improve the grease content of arabidopsis seeds and leaves; therefore, the technical scheme provided by the invention can be used for improving the oil content of the plant seeds and the nutritional tissues, and shows the application prospect in improving the oil content of the seeds and the nutritional tissues.

The invention discloses a protein complex identification method based on knowledge enhancement graph comparative learning, and belongs to the technical field of bioinformatics. Edge trimming is performed by using a gene expression profile and subcellular localization data, so that a knowledge enhancement PPI network is constructed; and incorporating the molecular function and the biological process as network attributes as attributes for enhancing the network. Graph structure enhancement is applied to knowledge enhanced PPI attribute networks, and multiple perturbation views are generated with diverse random perturbation policies including edge addition, edge discarding, and feature masking to help mitigate data sparsity. A perturbation view generated by graph structure enhancement is coded through a randomized graph convolutional network layer, so that multi-scale features are extracted, key PPI interaction is reserved, and meanwhile prediction performance is improved. And finally, the original PPI network is weighted again, and the protein complex is identified by adopting a core-affiliated strategy.

The invention relates to the technical field of biomedicine detection reagent manufacturing and reproductive medicine, in particular to an embryonic development potential sperm key protein kit and a use method thereof. The method is characterized in that an antibody combination capable of specifically recognizing a group of key proteins related to embryonic development potential in human sperms is prepared, and the antibodies, a fluorescence labeled secondary antibody, a cell nucleus dye, a concentrated washing liquid, a mounting medium and a pre-coated control porous glass slide are assembled into the special detection kit. The kit can be combined with a multi-mode imaging system (such as a fluorescence / confocal microscope and a common optical microscope) and image analysis software to realize the traditional analysis of sperm morphology and movement ability, and can perform synchronous and accurate qualitative and quantitative analysis with the expression level and subcellular localization of the key protein. The invention provides an integrated molecular detection solution, and provides a powerful tool for sperm quality screening and embryonic development potential prediction in an assisted reproduction technology.

The invention discloses a cherry sugar transporter gene CpSWEET10 and application thereof, and belongs to the technical field of cherry gene engineering. A novel sugar transporter gene CpSWEET10 is obtained through cloning, the nucleotide sequence of the novel sugar transporter gene CpSWEET10 is shown as SEQ ID NO.1, and the coded amino acid sequence of the novel sugar transporter gene CpSWEET10 is shown as SEQ ID NO.2. The gene is highly expressed in cherry fruits, and the content of soluble solids and total sugar in mature fruits is remarkably positively correlated with the expression quantity of the soluble solids and the total sugar; through subcellular localization and sugar transport function defect type yeast mutant functional complementation experimental analysis, the protein coded by the CpSWEET10 gene is localized in a cytoplasmic membrane and has a sucrose transport function; the cherry sugar transporter gene CpSWEET10 disclosed by the invention can be applied to the improvement of the sugar degree character of the fruits, and a new gene resource is provided for molecular breeding for improving the high-sugar quality of the cherry fruits.

The invention discloses a self-adaptive multi-channel fusion lncRNA subcellular localization prediction method based on a reinforcement learning agent, and relates to a long-chain non-coding RNA subcellular localization prediction method. The method aims at solving the problems that sample heterogeneity is ignored and RNA-protein interaction characteristics are ignored due to the fact that a fixed feature fusion strategy is adopted in an existing method. According to the method, firstly, multi-modal feature extraction is carried out, original feature vectors are obtained through splicing, the original feature vectors are projected to a reinforcement learning state, actions are obtained through an intelligent agent obtained through PPO training based on near-end strategy optimization, and the actions comprise feature selection mask codes, fusion weight guidance and dynamic hyper-parameter configuration. A personalized fusion strategy is obtained through action vectors generated by the reinforcement learning agent, the fusion prediction network fuses the features in the multi-modal feature set based on the personalized fusion strategy, and finally the positioning probability of the lncRNA at each subcell position is obtained.

This invention discloses the application of the apple MdWRI1 gene in regulating the wax content of apple fruits and leaves, belonging to the field of plant genetic engineering technology. This invention isolates an MdWRI1 gene with high expression levels in apple varieties with high wax content, its nucleotide sequence of which is shown in SEQ ID NO.1. Subcellular localization revealed that the transcription factor expressing the MdWRI1 gene is located in the cell nucleus. Experimental results show that overexpression of the MdWRI1 gene can significantly increase the wax content of apple fruits and leaves by promoting wax biosynthesis, indicating that the MdWRI1 gene plays a key role in regulating the wax content of apple fruits and leaves. This invention provides an efficient and rapid approach for apple breeding, provides genetic material for improving apple quality, and has broad application prospects in improving the economic and ecological benefits of apple cultivation.

The invention relates to an NtWUN gene as well as an expression product, an expression vector and application of the NtWUN gene in regulation and control of plant glandular hair development, and aims to solve the technical problems that the development mechanism of different types of epidermal hairs in plants (such as tobacco) is unknown and effective molecular regulation and control measures and utilization ways are lacked at present. According to the invention, an NtWUN gene for negatively regulating epidermal hair development is identified, a gene editing vector and an overexpression vector are constructed, and a gene editing homozygous strain and an overexpression homozygous strain are obtained through screening; tissue expression, MeJA response mode analysis, subcellular localization, plant phenotypic characteristic identification and the like show that the NtWUN can negatively regulate the occurrence of epidermal hair and influence the change of the total density of the epidermal hair, and the NtWUN has important application value in regulating the development process of the plant epidermal hair.

The invention discloses application of a corn RTA9 gene in enhancing aphid resistance of plants in the technical field of plant genetic engineering, a CDS sequence of the corn RTA9 gene is shown as SEQ ID NO.1, an amino acid sequence for coding the corn RTA9 gene is shown as SEQ ID NO.2, subcellular localization shows that RTA9 is mainly localized in mitochondria, and it is known through research of the invention that the corn RTA9 gene can be used for improving aphid resistance of plants. The function deletion of the RTA9 gene weakens the capability of producing ROS (reactive oxygen species) in response to aphid infection of the corn, so that the aphid resistance of the corn is reduced. The content of ROS (reactive oxygen species) in cells can be remarkably increased by overexpressing RTA9 in corn, meanwhile, the aphid resistance of corn is improved, and a theoretical basis and gene resources are provided for developing aphid-resistant crop strains.

The invention discloses a cell wall associated kinase gene VvWAKL8 for regulating and controlling texture formation of grape fruits and application thereof, the cell wall associated kinase gene VvWAKL8 is cloned from the grape fruits for the first time, the nucleotide sequence of the cell wall associated kinase gene VvWAKL8 is as shown in SEQ ID NO.1, and the coded amino acid sequence is as shown in SEQ ID NO.2. Research finds that the VvWAKL8 is specifically and highly expressed in the hard-pulp grape variety, and subcellular localization analysis shows that the protein is localized in a cell membrane; instantaneous overexpression of VvWAKL8 in grape fruits can significantly improve fruit hardness and regulate pectin metabolism related gene expression, and verifies the core function of VvWAKL8 in fruit texture formation. The research reveals that the VvWAKL8 is a key gene for regulating and controlling the grape fruit texture for the first time, a brand new gene resource is provided for molecular marker-assisted breeding of a new storage-resistant grape variety, and an important foundation is laid for deeply analyzing a cell wall signal to regulate and control a molecular mechanism of fruit development.

The application discloses application of an apple MdMYS1 gene in regulation of wax content of plant fruits and leaves and belongs to the technical field of plant genetic engineering. The application separates a MdMYS1 gene with high expression in an apple variety with high wax content, and the nucleotide sequence of the MdMYS1 gene is shown as SEQ ID NO. 1. Subcellular localization shows that a transcription factor expressed by the MdMYS1 gene is located on a cell nucleus. Experimental results show that overexpression of the MdMYS1 gene can significantly improve the wax content of apple fruits and leaves by promoting biosynthesis of the wax, and the MdMYS1 gene plays a key role in regulation of the wax content of apple fruits and leaves. The application provides an efficient and rapid way for apple breeding, provides gene materials for improvement of apple quality, and has wide application prospects in improvement of economic and ecological benefits of apple planting.

The invention discloses a functionalized targeting fluorescent nanoprobe and application thereof in protein mutation analysis, and relates to the technical field of biology, the fluorescent nanoprobe comprises a targeting molecule-PEG hydrophilic bridging molecule and a matrix; the preparation method comprises the following steps: adding nano-seeds into fluorescent molecules to prepare fluorescent nano-particles, and stirring and mixing the fluorescent nano-particles with targeting molecules-PEG hydrophilic bridging molecules; the method is combined with deep learning to be applied to protein mutation analysis. The multifunctional integrated protein mutation receptor targeted fluorescent nanoprobe provided by the invention can accurately mark the position of a mutant protein at a single cell level, and can realize in-situ, real-time and dynamic living cell imaging of a tumor cell endogenous molecular mutant protein; and qualitative and quantitative analysis of tumor cell protein mutation can be realized through subcellular localization of the targeted fluorescent nanoprobe.

The invention discloses a method for detecting subcellular localization of a functional gene by using a tea tree leaf, a target gene is expressed in the tea tree leaf through an agrobacterium transformation method, the method has the advantages of short period, rapidness, high conversion rate, low cost, easiness in operation and the like, and the localization condition of the target gene in the tea tree leaf can be more intuitively and accurately observed; and important technical support is provided for tea tree gene function research. Compared with mode plants such as tobacco and arabidopsis thaliana, the novel subcellular localization experimental material has the advantages that the tea tree leaves are used for observing subcellular localization, and a more intuitive and more accurate mode is provided for gene function research in tea trees. In addition, the method is short in conversion period, fluorescence signals can be observed 36-48 hours after conversion, and the method is fast and efficient.

The invention discloses application of a SbMYB113-like gene to improvement of low-temperature-resistant breeding of scutellaria baicalensis, belongs to the technical field of plant biology, is used for scutellaria baicalensis, and is characterized in that the nucleotide sequence of the SbMYB113-like gene is shown as SEQ ID NO: 1, by reducing overexpression of the SbMYB113-like gene in the scutellaria baicalensis, the anti-oxidation defense system of the scutellaria baicalensis is improved, the stability of the cell structure and function is protected, and the stability of the low-temperature-resistant breeding of the scutellaria baicalensis is improved. The tolerance of the scutellaria baicalensis to low-temperature stress is improved. The invention aims at researching subcellular localization of SbMYB113-like protein, analyzing tissue specific expression and a low-temperature stress response expression mode of the SbMYB113-like gene in combination with real-time fluorescent quantitative PCR (Polymerase Chain Reaction), and verifying the cold resistance function of SbMYB113-like through genetic transformation, so that the SbMYB113-like gene has an application value in low-temperature scutellaria baicalensis breeding.

The invention discloses a drug delivery system containing loaded adriamycin as well as a preparation method and application of the drug delivery system. The drug delivery system comprises poly (beta-amino ester) coupled with adriamycin, wherein the surface of the poly (beta-amino ester) is coated with dextran sulfate, and the poly (beta-amino ester) takes a fourth-generation lysine dendritic molecule Lys-G4 as a core. The drug delivery system has a pH-dependent drug release characteristic, has remarkable toxicity to C6 and U87MG glioma cells, has the capabilities of inducing apoptosis, inhibiting migration and targeting subcellular localization, and can effectively penetrate through a blood-brain barrier model and 3D tumor spheres. The delivery system is compounded with PLA-PEG-PLA thermosensitive hydrogel, and the thermosensitive hydrogel with a drug storage function is successfully constructed. Good tumor inhibition effect and biocompatibility are shown in nude mouse subcutaneous and in-situ glioma models. The invention not only provides a new strategy for overcoming the blood brain barrier, but also can effectively reduce the systemic toxicity, and opens up a new research thought and method for glioma treatment.

This invention discloses a Panax notoginseng ethylene response factor gene. PnERF120 Its nucleotide sequence is shown in SEQ ID NO:1, and the encoded amino acid sequence is shown in SEQ ID NO:2. PnERF120 The gene originates from Panax notoginseng, and the encoded protein sequence contains a typical conserved domain of the ERF transcription factor. This invention demonstrates, through gene cloning, bioinformatics analysis, expression pattern analysis, subcellular localization, overexpression, and other techniques and methods, that... PnERF120 This invention can be used to improve the resistance of Panax notoginseng to Panax notoginseng A virus, and also to improve the resistance of tobacco to tobacco mosaic virus. This invention provides new functional genes and application pathways for antiviral breeding and disease-resistant materials of Panax notoginseng and other plants.

The invention discloses an exocytosis protein subcellular localization method for mannitol-induced plasm-wall separation, and relates to the technical field of cytobiology, the method comprises the following specific steps: constructing a fusion expression vector: connecting a plant target exocytosis protein gene with a fluorescent protein gene through a gene cloning technology to obtain a fusion expression vector; inserting into a plant expression vector to obtain a recombinant expression vector containing the target extracellular secretory protein-fluorescent protein fusion gene; performing bacterial colony PCR identification and sequencing verification on the recombinant expression vector; by adopting an agrobacterium tumefaciens-mediated plant leaf instantaneous transformation technology, the fusion gene is rapidly expressed in plant cells, subsequent experiments can be carried out after culture is carried out for 2-3 days, the experiment period is greatly shortened, meanwhile, leaf cells are induced to be subjected to plasmon-wall separation through a gradient mannitol solution, positive cell screening and identification are not needed, and the application prospect is wide. And positioning can be realized by directly observing fluorescence signal distribution by using a laser confocal microscope.

The application belongs to the field of subcellular localization prediction, and provides an lncRNA subcellular localization prediction method and system for unbalanced data, in order to fully utilize the sequence information of lncRNA, physical and chemical mode features and distributed representation features of nucleic acids are extracted, and two basic classifiers including a convolutional neural network and a gated recurrent unit are integrated, a subcellular classification result is obtained through adaptive weighted averaging of the two models, and subcellular localization is carried out based on the classification result, so that the precision of subcellular localization prediction on an unbalanced data set can be improved. In order to solve the problem that the model performs poorly on the unbalanced data set, a label distribution margin perception loss function is used in the training process. Compared with traditional machine learning models and existing predictors, the method has stronger tolerance to class imbalance and robustness.

The invention relates to the field of plant genetic engineering, in particular to a rosa chinensis high-temperature-resistant gene RcbHLH59 and application thereof.The rosa chinensis high-temperature-resistant gene RcbHLH59 is identified and cloned to obtain the RcbHLH59 gene, and a CDS sequence and a coded amino acid sequence of the RcbHLH59 gene are obtained; secondly, constructing an overexpression vector and a VIGS silencing vector of the gene, performing agrobacterium-mediated transformation on the Chinese rose, verifying that the gene is a nuclear transcription factor in combination with subcellular localization, and verifying that leaves of the Chinese rose overexpressing the gene are dark green and upright, leaves of the Chinese rose silencing the gene are more serious in yellowing and wilting through a high-temperature stress experiment; according to the rosa chinensis high-temperature-resistant gene RcbHLH59 and the application thereof, eight related genes capable of regulating eight high-temperature response metabolic pathways can be regulated, rosa chinensis heat-resistant gene resources can be enriched, a key target is provided for rosa chinensis high-temperature-resistant molecular breeding, the growth vigor and ornamental value of rosa chinensis at high temperature are effectively improved, and popularization and application of the rosa chinensis in landscaping are promoted.

The application discloses a preparation method and application of a hollow manganese dioxide nanocarrier coated with a human umbilical cord mesenchymal stem cell membrane and a transmembrane peptide TAT, and is characterized in that: hollow mesoporous manganese dioxide is synthesized through a selective etching technology, and a layer of human umbilical cord mesenchymal stem cell membrane is coated outside the manganese dioxide through a repeated extrusion method. Since the cell membrane has special tumor homing characteristics, and lacks major histocompatibility complex-II and only expresses major histocompatibility complex-I in a trace amount, the key scientific problems and difficulties such as low biological safety, strong immunogenicity and easy elimination by the body of artificially synthesized nanomaterials can be effectively solved. Finally, the cell membrane surface is modified with the transmembrane peptide TAT through a phospholipid insertion method, precise subcellular localization-nucleus targeting is realized, and tumor cells can be more effectively killed.

The application belongs to the field of molecular biological technology, and particularly relates to CeABI3B a gene, an expression vector and application thereof in plant oil regulation. CeABI3B The application provides a gene for the first time, a nucleotide sequence of which is shown as SEQ ID NO. 1, and a coding region of which has a full length of 1707 bp. CeABI3B A series of vectors containing the gene are constructed for the first time, including subcellular localization, yeast hybridization and plant overexpression, and it is proved that the CeABI3B protein has a transcription activation function and is located in the cell nucleus, which is in line with the basic characteristics of transcription factors. CeABI3B Not only is it found that transient overexpression of the gene can significantly improve the oil content of tobacco leaves, but also it is proved that overexpression of the gene can significantly improve the oil content of transgenic Arabidopsis thaliana seeds and leaves. CeABI3B Therefore, the cloned and identified gene can be used to improve the oil content of plant seeds and nutrient tissues, and has a wide application prospect.

The application provides a protein clustering method combining information granulation and evidence reasoning, which comprises the following steps: granulating protein sequence data in cells, fusing information granules of the protein sequence data based on intersection relationship, fusing granule clusters of the protein sequence data based on density propagation, fusing granule groups of the protein sequence data based on distance, calculating evidence values of stable samples, calculating evidence values of unstable samples, assigning categories of the protein sequence samples according to the evidence values, and improving the accuracy of protein subcellular localization prediction, so as to facilitate biologists to judge or design specific proteins in each subcellular structure or to predict the function of the proteins.

The application discloses application of TRIM11 protein in preparation of a medicine for treating spinocerebellar ataxia 51. The application adopts an SCA51 specific cell model, and identifies, through a large number of screening experiments, that the TRIM11 protein has the function of significantly removing mutant THAP11 protein and aggregates thereof. Experimental data show that the mutant protein removal efficiency of the TRIM11 treatment group is significantly better than that of the control group. The application verifies the removal effect of TRIM11 on the SCA51 pathological protein from the two dimensions of protein expression level and subcellular localization through Western blotting quantitative analysis and immunofluorescence co-localization technology. The three-dimensional reconstruction image clearly shows that the mutant protein aggregates are significantly reduced. The application first establishes a complete research system from target discovery to mechanism analysis, and provides a new intervention strategy for precise treatment of SCA51.

The present application belongs to the field of chemical synthesis and biotechnology, and particularly relates to a kind of sugar metabolism product molecular probes and preparation method thereof. Starting from fructose, a bio-orthogonal group is added to the 4-position hydroxyl group of the compound without affecting the biological activity of the sugar metabolism product. The present application provides a kind of sugar metabolism product probe and preparation method thereof, compared with the prior art, has the following beneficial effects: (1) the present application first synthesizes an endogenous sugar metabolism product molecular probe that can undergo click chemistry reaction, by adding a bio-orthogonal group to the 4-position hydroxyl group, the biological activity of the sugar metabolism product is maintained, which can be used for live cell imaging; (2) the probe of the present application has not been reported, and the synthesis method has less side reaction, mild reaction condition, good selectivity, can reflect the behavior of endogenous sugar metabolism product in cells, including direct reaction of sugar metabolism product in live cells Subcellular localization and tracing, is an important technical support for the study of live cell sugar metabolism.

The application discloses a kind of lncRNA subcellular localization prediction method based on lightGBM, comprising, first, the nucleotide of the front section multiple of known lncRNA sequence is intercepted as sequence sample one;Then by based on single strand multiclass position-specific three nucleotide bias and reverse complementary kmer, sequence sample one is respectively characterized coding, the combination of two kinds of feature coding is vector;Use lightGMB as learning algorithm;Using 5-fold cross validation optimization reverse complementary kmer and LightGBM's hyperparameter;Most intercept unknown lncRNA sequence front end multiple bases as sequence sample two, and its single strand multiclass position-specific three nucleotide bias and the combination of optimized reverse complementary kmer feature coding is input in trained lightGBM, will obtain its localization subcellular type, the patent of the application can be according to long-chain non-coding RNA sequence prediction in cytoplasm, nucleus, ribosome, cytosol, exosome five subcellular positions, the application realizes simply, and prediction precision is high.

The application discloses a transcription factor SrMYB1 for regulating synthesis of steviol glycoside, an expression protein thereof and application, and belongs to the technical field of plant genetic engineering. The transcription factor SrMYB1 has a nucleotide sequence as shown in SEQ ID NO. 1, and the amino acid sequence of the expression protein is as shown in SEQ ID NO. 2. Subcellular localization in tobacco epidermal cells shows that the protein coded by the gene is mainly located in the nucleus; yeast activity experiment shows that the protein has transcription activation activity. Yeast single hybridization experiment shows that SrMYB1 can be combined on the promoter of a key gene for synthesis of steviol glycoside SrUGT76G1 . Promoter activity and transgenic experiment both show that SrMYB1 significantly inhibits transcription of the key gene for synthesis of steviol glycoside SrUGT76G1 . Based on the characteristics, certain reference can be provided for quality regulation of steviol glycoside and cultivation of new steviol varieties.

The invention relates to a recombinant microbial strain for de novo synthesis of gardenin B and application of the recombinant microbial strain in production of polymethoxylated flavone compounds, and belongs to a cross technology of synthetic biology and organic synthetic chemistry. The invention designs and constructs a heterozygous gardenin B biosynthesis pathway, and obtains a microbial strain for synthesizing gardenin B from the beginning. By screening F8H and redox partner types and regulating and controlling subcellular localization, an F8H-redox partner combination with optimal space and type is determined, and an engineering strain of which the gardenin B yield is greatly improved is obtained. The efficient synthesis method of the polymethoxylated flavonoids is established by selecting recombinant microorganisms for synthesizing gardenin B from de novo to perform co-culture fermentation or chemical conversion of fermentation products.

The invention provides a preparation method and application of artemisia selengensis protoplast, and belongs to the technical field of plant cell research. The preparation method of the Artemisia selengensis protoplast comprises the following steps: S1, cutting tender leaves of Artemisia selengensis into filaments, and completely immersing the filaments in a 13% CPW salt solution for pretreatment for 1 h; and S2, adding the artemisia selengensis tissues pretreated in the step S1 into an enzymatic hydrolysate for enzymolysis, centrifuging after enzymolysis is finished, collecting protoplast, resuspending the protoplast by using a W5 salt solution, and superposing the obtained resuspension on a sucrose solution for centrifugation to obtain the purified artemisia selengensis protoplast. Wherein the enzymatic hydrolysate contains 3wt% of cellulase and 0.8 wt% of macerozyme. The method successfully prepares a large amount of high-activity Artemisia selengensis protoplast. Based on the protoplast, an efficient and stable Artemisia selengensis protoplast instantaneous transformation system is established, subcellular localization of Artemisia selengensis AsPAL gene is successfully realized, and an important technical basis is provided for Artemisia selengensis gene function research and gene editing application.

The invention relates to a wheat low-phosphorus response transcription factor TaERF112 and application thereof, and belongs to the technical field of plant bioengineering. The low-phosphorus response transcription factor TaERF112 gene is cloned from wheat, the CDS region sequence of the gene is 882bp, 294 amino acids are encoded, the molecular weight is 31 and 572.92 Da, and the gene is hydrophilic protein. By analyzing the TaERF112 gene structure, protein physicochemical properties, subcellular localization and species evolution characteristics, the characteristics and function prediction of the gene are known. Furthermore, a wheat taerf112 mutant is created by using a CRISPR / Cas9 technology, phenotypic characters, biomass and phosphorus utilization characteristics of a transformed strain under different phosphorus conditions are evaluated, and the important effect of the wheat taerf112 mutant in regulation and control of a wheat root system and phosphorus homeostasis is preliminarily revealed. Theoretical basis and gene resources are provided for efficient genetic improvement of wheat phosphorus.