15 results about "Complex formation" patented technology

Described are affinity purification system that includes a carrier / surface that is non-cellular, and sliding clamp (SC) protein, and methods for purifying proteins that bind to the SC. The SC is associated with the carrier / surface via covalent / non-covalent interactions. To attain control of coupling site, the SC can be mutated via site-directed mutagenesis to introduce an exogenous residue and, the exogenous internal residue is conjugated to the non-cellular surface through the linker. The SC can also be coupled to the carrier via non-covalent interactions such as the affinity interactions involved in ligand / binding partner complex formation.The SC-based affinity purification system are used in a purification column as bait proteins, to isolate SC binding partners or non SC-binding proteins engineered to contain a SC binding site prior to its purification.

The technology provided herein relates to multiplex methods and kits for detecting different analytes in a sample simultaneously, in particular by using premixed macromolecule complexes like complexes having a primary and a secondary antibody, wherein the primary antibodies contain identical identifier elements.

A method produces an aqueous liquid preparation for magnetic resonance contrast that contains a complex of a lanthanide and a macrocyclic chelate together with free chelate. A solution of the macrocyclic chelate in water is prepared, lanthanide and meglumine are added, and complex formation proceeds in the presence of meglumine at a pH between 6.5 and 8.0 with lanthanide in molar excess over meglumine and meglumine in molar excess over the chelate. The pH is then reduced to a target value below 5.0 by incremental addition of an acid such as further chelate, optionally followed by addition of a fixed chelate amount. The pH is subsequently raised above 6.5 with meglumine and the solution is diluted to a final volume, providing an industrial-scale single-vessel process that yields a preparation containing a defined low amount of free lanthanide.

Provided is a method for recovering an organic fluorine compound which requires only a small amount of acid and base for recovery treatment. A metal cation is added to an aqueous phase containing an organic fluorine compound to cause a complex formation reaction with molecules of the organic fluorine compound, and an electrically neutral chemical species generated thereby is solvent-extracted into an organic phase forming a two-liquid system with the aqueous phase.

This invention belongs to the field of pharmaceutical technology, specifically relating to a method for improving the water solubility of drugs by mutually solubilizing rosiglitazone and ibuprofen. The method involves co-dissolving rosiglitazone and ibuprofen in an aqueous solution, resulting in an increase in the water solubility of both drugs due to the presence of the other. This method is simple to operate, low in cost, and the solubilizing effect is due to intermolecular interactions between the two drugs within the solution system; no chemical reaction or co-crystal complex formation occurs between the two drugs.

This invention relates to an ultra-micro associative emulsion modifier, belonging to the technical field of chemical flooding agents used in oilfield chemistry to enhance oil recovery. It addresses the problems of insufficient interfacial activity, poor emulsion stability, and limited permeability in formation pores caused by improper surfactant formulation in existing modifiers. The key technical point is to provide a composite system composed of water, polyether-modified polysiloxane, sodium α-olefin sulfonate, and sodium dodecyl sulfate in specific weight proportions. Through molecular synergy and association among the components, this system spontaneously forms ultra-fine and stable emulsion droplets, effectively reducing the oil-water interfacial tension and enhancing the long-term stability of the system in complex formation environments. Its main application is as a modifier in oilfield water injection development, adjusting the water flow profile by injecting it into the formation, emulsifying and carrying residual oil, thereby improving oil displacement efficiency and increasing the ultimate oil recovery rate.

The present invention discloses an monoclonal antibody, which can bind to HyIL-6 with the binding constant 2.86×10−10 and significantly inhibit IL-6 / IL-6R / gp130 complex formation. In addition, the monoclonal antibody of the present invention effectively inhibits HyIL-6-stimulated signal transducer and activator of transcription 3 (STAT3) activation and related vascular endothelial growth factor (VEGF) induction. Data from hydrogen deuterium exchange mass spectrometry (HDX-MS) demonstrate that the antibody of the present invention mainly binds to site IIIa of IL-6 and blocks the final step in the interaction between gp130 and IL-6 / IL-6R complex. Additionally, data from ELISA binding assays and kinetics assays indicate that the antibody of the present invention interacts simultaneously with IL-6 and IL-6R, while it does not interact with IL-6R alone. The unique features of the antibody of the present invention offer a novel alternative for IL-6 blockade and illuminate a better therapeutic intervention targeting IL-6.

This invention discloses a method for preparing amphoteric electrodeposition photoresist on a metal surface, specifically including the following steps: Step 1, synthesizing cashew nut shellac monomer; Step 2, preparing an amphoteric resin; Step 3, preparing an electrophoretic coating; Step 4, electrophoretically depositing photoresist on a conductive substrate. This invention also discloses the preparation method and application of amphoteric electrodeposition photoresist on a metal surface. This invention solves the problems of existing photoresists causing complex formation due to strong alkalis, corrosion of metal circuits by strong acids, and swelling of the photoresist film.

The invention provides a thermal response type phase change microcapsule, a preparation method thereof and a water-based drilling fluid. The thermal response type phase change microcapsule comprises a core material and a shell layer, the core material is an eutectic substance containing carboxylated carbon nanotubes, the shell layer is silicon dioxide containing graphene oxide, and the carboxylated carbon nanotubes and the graphene oxide are directionally arranged to form a continuous heat conduction path from the core material to the shell layer. The thermal response type phase change microcapsule prepared by the invention can be directly compatible in an existing drilling fluid system, shows good dispersity and suspension property, is fast in thermal response and strong in adaptability, is suitable for extreme drilling environments such as underground temperature control, complex formation thermal management and the like, and has less damage to rheology and filtration performance.

This invention discloses a high-temperature resistant and calcium-sensitive cementing fluid loss reducing agent. This fluid loss reducing agent is a copolymer with a micro-crosslinked stable structure, prepared by free radical copolymerization of sulfonic acid-containing olefin monomers, tertiary amine ester functional monomers, amide-containing olefin monomers, cyclic functional monomers, and crosslinked monomers. The cyclic monomers lock the main chain conformation, and the crosslinked network provides spatial protection, significantly enhancing the stability of the ester side chains at the physical level. This fundamentally inhibits the formation of harmful carboxyl groups, improving the product's calcium sensitivity resistance and high-temperature resistance. This fluid loss reducing agent exhibits excellent high-temperature and strong alkali resistance, suitable for application in a wide temperature range of 80℃ to 220℃, alkaline environments with pH 8 to 12, and high calcium ion concentrations. It also shows good compatibility with cement slurry, without adversely affecting its rheological properties or thickening time. It can be widely used in cementing operations in complex formations such as deep onshore wells, high-temperature and high-pressure offshore wells, and salt-gypsum layers.

The invention relates to the field of oilfield chemistry, in particular to a low-loss oilfield blocking remover and a using method thereof. Comprising the following raw materials in parts by weight: 5-30 parts of a modified flaky ferric hydroxide material, 1-10 parts of 3-hydroxy-2-[(phosphono formyl) amino] sodium propionate, 0.5-5 parts of a surface regulating agent, 0.5-3 parts of a slow release regulator, 0.2-2 parts of a pH buffer agent, 0.5-3 parts of a biodegradable stabilizer and 47-92.3 parts of deionized water, and through interface regulation and control and structural stability design, the blockage removal treatment on the reservoir blockage is effectively realized. The system can reduce secondary damage to strata while maintaining a good permeability improvement effect, has excellent viscosity retentivity and mineralized water stability, and is outstanding in emulsification and dispersion performance, and the overall performance shows higher applicability and persistence under complex stratum conditions.

To provide a method for simply and selectively separating and recovering high-purity dysprosium from a solution containing neodymium ion and dysprosium ion whose simple separation is difficult.SOLUTION: A dysprosium complex is created by allowing a tripod-type Schiff ligand to react with neodymium ion and dysprosium ion in a mixed solvent of an aprotic polar solvent and a protic polar solvent. By fractionally precipitating the dysprosium complex, a separation condition for precipitating the dysprosium complex as a crystal in 1 step is determined, and dysprosium is separated at purity of 99% or more.SELECTED DRAWING: None

The present invention relates to nanostructure-bonding partner conjugates, as well as reaction mixtures, analyte detection devices, and methods for fabricating and using conjugates. [Solution] In particular, the present invention provides a method for detecting a target analyte in a sample, comprising the step of mixing the sample with a first detection conjugate and a second detection conjugate in a solution, wherein the first and second detection conjugates include metal nanostructures linked to binding partners that can specifically bind to the target analyte when it is present in the sample, forming a complex between the first detection conjugate and the analyte and the second detection conjugate, and the change in optical signal during complex formation indicates the presence of the target analyte in the sample.

A method of manufacturing a semiconductor device in a semiconductor body having a first surface and a second surface is proposed. Semiconductor device elements are formed in the semiconductor body by processing the semiconductor body at the first surface. A wiring area is formed over the first surface of the semiconductor body. The semiconductor body is attached to a carrier via the wiring area. Thereafter, ions are implanted through the second surface into the semiconductor body. The ions are ions of a doping element, or ions, which induce doping by complex formation, or ions of a heavy metal. A surface region of the semiconductor body at the second surface is irradiated with a plurality of laser pulses. Thereafter, the carrier is removed from the semiconductor body.

Compositions and methods for the selective extraction of rare molecular species from a mixture containing a predominance of related molecular species, and their subsequent release are provided. Compositions include a segregating probe having a target-specific targeting portion, a protecting portion, a scissile or cleavable site or portion, and a capture portion. Complex formation between the targeting portion and the target rare species results in protection of the cleavable site. Such complexes remain intact through a cleavage step directed to the cleavable site or portion of the segregating probe. While complex formation can occur between a segregating probe and a related, but non-target, species the cleavable site is not protected in such complexes. A subsequent capture step directed to the capture portion selectively captures or segregates the target rare species from the general milieu, which can be removed by washing. Subsequent release of the target rare species from the segregating probe provides a solution enriched in the target rare species.