32 results about "Complex formation" patented technology

The preparation method comprises the following steps: mixing acrylamide, acrylic acid, 2-methyl-2-acrylamido propanesulfonic acid, N, N-dimethylacrylamide, a modified additive, modified montmorillonite and deionized water, and polymerizing under the action of an initiator, so as to obtain the degradable fracturing fluid composite thickening agent. Compared with traditional guar gum, cyclodextrin molecules contained in the modified additive have better high-temperature-resistant effect and good anti-shearing effect, so that the thickening agent shows a stable chemical structure and good rheological property and sand-carrying capacity in the high-temperature use process, dissolution of a hydraulic fracturing fluid to a salt layer can be greatly relieved, and the thickening effect of the thickening agent is improved. Meanwhile, no residue is generated after gel breaking, damage to a reservoir stratum can be effectively reduced, the salt resistance of the thickening agent can be improved through the contained polypropylene glycol structure, the thickening agent can be suitable for the complex stratum environment, meanwhile, the organic part in the thickening agent can be degraded, and then the possibility of pollution to soil and water is reduced.

The present invention discloses a degradable fracturing fluid composite thickener and a preparation method thereof. By mixing acrylamide, acrylic acid, 2-methyl-2-acrylamidopropanesulfonic acid, N,N-dimethylacrylamide, a modified additive, modified montmorillonite and deionized water, and polymerizing under the action of an initiator, the modified additive contains cyclodextrin molecules. Compared with traditional guar gum, cyclodextrin has better high-temperature resistance and good shear resistance, enabling the thickener to exhibit a stable chemical structure, good rheological properties and sand-carrying capacity during high-temperature use. Thus, it can greatly reduce the dissolution of the salt layer by the fracturing fluid, and at the same time, there is no residue after gel breaking, which can effectively reduce the damage to the reservoir. The polyvinyl alcohol structure contained can increase the salt resistance of the thickener, enabling the thickener to be applicable to complex formation environments. At the same time, the organic part in the thickener can be degraded, thereby reducing the possibility of pollution to soil and water bodies.

The invention provides preparation and application of an ethylene selective oligomerization catalyst composition. The ethylene selective oligomerization catalyst composition comprises a transition metal compound a, a ligand b and an auxiliary c. The novel indole onium iodized salt ligand is synthesized for the first time, and when an ethylene oligomerization catalyst system taking the ligand compound as the ligand is used for catalyzing ethylene oligomerization reaction, the ligand compound has the advantages of high coordination capability, proper electrical property and capability of forming a stable complex with transition metal; the ligand can resist high temperature, reduces influence on 1-octene selectivity and catalyst activity as much as possible, and has an excellent application prospect.

The present invention discloses a fracturing and sealing device and method based on supercritical CO2 injection into basalt. The fracturing and sealing device includes: a test body module, including a basalt sample; an injection module, which injects the required gas, liquid or gas-liquid mixture into the test body module; a pressure loading module, which is connected to the test body and applies axial pressure and / or confining pressure to the test body module; wherein the injection module and the pressure loading module are configured to achieve fracturing of the basalt sample, as well as the radial permeability of the basalt sample after fracturing and the sealing of CO2 by the basalt sample after fracturing. It can overcome the limitations of the single function of traditional indoor test devices and achieve a comprehensive understanding of the fracturing and sealing process under complex formation environmental conditions; it is also convenient for analyzing the formation law of basalt fracture networks, evaluating the permeability of fracture networks, and comparing the feasibility and effectiveness of basalt mineralization and CO2 storage under different injection modes.

The present invention discloses an experimental device and method for studying the wax deposition law in porous media in near-wellbore areas, belonging to the technical field of wax deposition experiments. The experimental device comprises a wax deposition visualization model, a multi-stage temperature control module, a liquid injection and cleaning module, a confining pressure adjustment module, a back pressure control module, a product recovery module and an image acquisition module; a natural core slice with a pore throat structure is simulated by a wax deposition visualization model, a multi-stage temperature control module is used to perform graded local heating to simulate complex formation temperature conditions, a confining pressure adjustment module is used to provide confining pressure, a back pressure control module is used to adjust the model outlet pressure to simulate bottom hole flow pressure, a liquid injection and cleaning module is used to inject crude oil and cleaning fluid therein, and an oil displacement experiment and subsequent cleaning are completed; an image acquisition module and a visualization clamping module are used to intuitively observe the wax deposition process in the model, and the wax crystal phase change process during the experiment is analyzed, and the wax deposition formulas of different parts of the model are used to characterize the step-by-step precipitation law of wax.

The present invention provides methods for quantifying and / or screening for the formation of complexes, in which two of the three compounds of the complex are proteins, one of the two tagged with a fluorescent label and the other of the two tagged with a fluorescence quencher. The third compound is a compound capable of linking those two proteins together, thus forming a ternary complex. The methods of the present invention consist of analysing the changes in fluorescence spectrum that occur following the formation of a complex comprising said compounds, due to the interaction between fluorescent label and fluorescence quencher that formation of said complex brings.

Described are affinity purification system that includes a carrier / surface that is non-cellular, and sliding clamp (SC) protein, and methods for purifying proteins that bind to the SC. The SC is associated with the carrier / surface via covalent / non-covalent interactions. To attain control of coupling site, the SC can be mutated via site-directed mutagenesis to introduce an exogenous residue and, the exogenous internal residue is conjugated to the non-cellular surface through the linker. The SC can also be coupled to the carrier via non-covalent interactions such as the affinity interactions involved in ligand / binding partner complex formation.The SC-based affinity purification system are used in a purification column as bait proteins, to isolate SC binding partners or non SC-binding proteins engineered to contain a SC binding site prior to its purification.

The technology provided herein relates to multiplex methods and kits for detecting different analytes in a sample simultaneously, in particular by using premixed macromolecule complexes like complexes having a primary and a secondary antibody, wherein the primary antibodies contain identical identifier elements.

The present invention relates to nanostructure-binding partner conjugates, as well as reaction mixtures, analyte detection devices, and methods of making and using the conjugates. In particular, the invention provides a method of detecting a target analyte in a sample comprising mixing the sample with a first detection conjugate and a second detection conjugate in solution, wherein the first and second detection conjugates comprise metallic nanostructures coupled to binding partners that are capable of specifically binding to the target analyte if present in the sample to form a complex between the first detection conjugate, the analyte, and the second detection conjugate, wherein a change in an optical signal upon complex formation indicates the presence of the target analyte in the sample.

This method for detecting a test substance in a sample by a single molecule detection method includes: a complex formation step for forming a complex that includes the test substance, a marker comprising a marker substance and a first probe molecule capable of binding to the test substance, and a capturing body comprising an insoluble carrier and a second probe molecule capable of binding to the test substance; a separation step for separating the marker from the complex; and a detection step for detecting the marker separated from the complex by the single molecule detection method. The equilibrium dissociation constant (KD1) between the test substance and the first probe molecule and the equilibrium dissociation constant (KD2) between the test substance and the second probe molecule are both less than 1.0×10-8 M. The separation step includes a step for heating the complex at 37°C or higher in a processing liquid containing at least one agent selected from the group consisting of a surfactant, a pH modifier, and a reducing agent.

A method produces an aqueous liquid preparation for magnetic resonance contrast that contains a complex of a lanthanide and a macrocyclic chelate together with free chelate. A solution of the macrocyclic chelate in water is prepared, lanthanide and meglumine are added, and complex formation proceeds in the presence of meglumine at a pH between 6.5 and 8.0 with lanthanide in molar excess over meglumine and meglumine in molar excess over the chelate. The pH is then reduced to a target value below 5.0 by incremental addition of an acid such as further chelate, optionally followed by addition of a fixed chelate amount. The pH is subsequently raised above 6.5 with meglumine and the solution is diluted to a final volume, providing an industrial-scale single-vessel process that yields a preparation containing a defined low amount of free lanthanide.

In a first aspect, the invention relates to a method for preparing a magnetic bead comprising at least one magnetic particle (M) and a silica coating, wherein the silica coating comprises at least two silica layers, the method comprising steps (a) to (d), wherein at least one of steps (b), (c) and (d) is / are done under sonication, wherein sonication is done with an amplitude (peak-to-peak) in the range of from 50 to 250 μm. A second aspect of the invention is related to a magnetic bead comprising (i) at least one magnetic particle (M) and (ii) a silica coating, wherein the silica coating comprises at least two silica layers; wherein the magnetic bead is stable against 7.5 M hydrochlorid acid and has a metal (cation) leaching rate in 7.5 M hydrochloric acid in the range of from 0.1 to 10%, wherein the metal (cation) leaching is determined according to a complex formation of Fe2+ with bathophenanthroline according to Reference Example 8.2. In a third aspect, the invention relates to a functionalized magnetic bead comprising at least one, magnetic bead according to the second aspect, wherein an outer silica layer is functionalized with at least one group selected from the group consisting of amino group, azide group, alkyne group, carboxyl group, thiol group, epoxy group, aryl group and alkyl group. A fourth aspect of the invention is directed to a process for functionalizing a magnetic bead according to the second aspect comprising at least one magnetic particle (M) and a silica coating, wherein the silica coating comprises at least two silica layers. A fifth aspect of the invention is related to a method for preparing magnetic Fe3O4 supra particles and a sixth aspect relates to the use of a magnetic bead according to the second aspect or of a functionalized according to the fourth aspect for immobilization of acid stable biocatalysts or for solid-phase organic synthesis using acid-stable linker.

Provided is a method for recovering an organic fluorine compound which requires only a small amount of acid and base for recovery treatment. A metal cation is added to an aqueous phase containing an organic fluorine compound to cause a complex formation reaction with molecules of the organic fluorine compound, and an electrically neutral chemical species generated thereby is solvent-extracted into an organic phase forming a two-liquid system with the aqueous phase.

The present invention relates to nanostructure-binding partner conjugates, as well as reaction mixtures, analyte detection devices, and methods of making and using the conjugates. In particular, the present invention provides a method of detecting a target analyte in a sample comprising mixing the sample with a first detection conjugate and a second detection conjugate in a solution wherein the first detection conjugate and the second detection conjugate comprise a metal nanostructure coupled to a binding partner, if the target analyte is present in the sample, the binding partner is capable of specifically binding to the target analyte to form a complex between the first detection conjugate, the analyte, and the second detection conjugate, wherein a change in the light signal upon formation of the complex is indicative of the presence of the target analyte in the sample.

This invention belongs to the field of pharmaceutical technology, specifically relating to a method for improving the water solubility of drugs by mutually solubilizing rosiglitazone and ibuprofen. The method involves co-dissolving rosiglitazone and ibuprofen in an aqueous solution, resulting in an increase in the water solubility of both drugs due to the presence of the other. This method is simple to operate, low in cost, and the solubilizing effect is due to intermolecular interactions between the two drugs within the solution system; no chemical reaction or co-crystal complex formation occurs between the two drugs.

This invention relates to an ultra-micro associative emulsion modifier, belonging to the technical field of chemical flooding agents used in oilfield chemistry to enhance oil recovery. It addresses the problems of insufficient interfacial activity, poor emulsion stability, and limited permeability in formation pores caused by improper surfactant formulation in existing modifiers. The key technical point is to provide a composite system composed of water, polyether-modified polysiloxane, sodium α-olefin sulfonate, and sodium dodecyl sulfate in specific weight proportions. Through molecular synergy and association among the components, this system spontaneously forms ultra-fine and stable emulsion droplets, effectively reducing the oil-water interfacial tension and enhancing the long-term stability of the system in complex formation environments. Its main application is as a modifier in oilfield water injection development, adjusting the water flow profile by injecting it into the formation, emulsifying and carrying residual oil, thereby improving oil displacement efficiency and increasing the ultimate oil recovery rate.

The present invention discloses an monoclonal antibody, which can bind to HyIL-6 with the binding constant 2.86×10−10 and significantly inhibit IL-6 / IL-6R / gp130 complex formation. In addition, the monoclonal antibody of the present invention effectively inhibits HyIL-6-stimulated signal transducer and activator of transcription 3 (STAT3) activation and related vascular endothelial growth factor (VEGF) induction. Data from hydrogen deuterium exchange mass spectrometry (HDX-MS) demonstrate that the antibody of the present invention mainly binds to site IIIa of IL-6 and blocks the final step in the interaction between gp130 and IL-6 / IL-6R complex. Additionally, data from ELISA binding assays and kinetics assays indicate that the antibody of the present invention interacts simultaneously with IL-6 and IL-6R, while it does not interact with IL-6R alone. The unique features of the antibody of the present invention offer a novel alternative for IL-6 blockade and illuminate a better therapeutic intervention targeting IL-6.

This invention discloses a method for preparing amphoteric electrodeposition photoresist on a metal surface, specifically including the following steps: Step 1, synthesizing cashew nut shellac monomer; Step 2, preparing an amphoteric resin; Step 3, preparing an electrophoretic coating; Step 4, electrophoretically depositing photoresist on a conductive substrate. This invention also discloses the preparation method and application of amphoteric electrodeposition photoresist on a metal surface. This invention solves the problems of existing photoresists causing complex formation due to strong alkalis, corrosion of metal circuits by strong acids, and swelling of the photoresist film.

The invention relates to a nanoparticle system synthesised for use in active targeting, consisting of 1st generation G1 peptide dendrimer with the chemical structure of Formula (I), 2nd generation G2 peptide dendrimer with the chemical structure of Formula (II), 3rd generation G3 peptide dendrimer with the chemical structure of Formula (III), 4th generation G4 peptide dendrimer with the chemical structure of Formula (IV), the core tof which consists of ethylene diamine, and branching units and surface functional groups of which consist only of L-arginine, and the synthesis method of this system. It relates to the nanoparticle system obtained as a result of the complex formation of any of the 3rd generation G3 peptide dendrimers from these dendimers, modified with folic acid with the chemical structure of Formula (V) by modification of the 3rd generation G3 dendrimer with the targeting molecule folic acid with β-ionone (4-(2,6,6-Trimethyl-1- cyclohexenyl)-3-buten-2-one) and the method of obtaining this system. They show anticancer properties by means of the nano formulation structure they create. Said nanoparticle has a cytotoxic effect against cancer cells, especially cervical cancer HeLa cancer cells.

The invention provides a thermal response type phase change microcapsule, a preparation method thereof and a water-based drilling fluid. The thermal response type phase change microcapsule comprises a core material and a shell layer, the core material is an eutectic substance containing carboxylated carbon nanotubes, the shell layer is silicon dioxide containing graphene oxide, and the carboxylated carbon nanotubes and the graphene oxide are directionally arranged to form a continuous heat conduction path from the core material to the shell layer. The thermal response type phase change microcapsule prepared by the invention can be directly compatible in an existing drilling fluid system, shows good dispersity and suspension property, is fast in thermal response and strong in adaptability, is suitable for extreme drilling environments such as underground temperature control, complex formation thermal management and the like, and has less damage to rheology and filtration performance.

This method for carbon dioxide fixation comprises: a step in which a metal complex of a metallic element with a chelating agent is formed in an aqueous alkali solution containing hydrogen carbonate ions or the like, the chelating agent, and a raw material including both the metallic element, which is capable of forming a carbonate with the hydrogen carbonate ions or the like and capable of reacting with the chelating agent to form a complex, and ions unnecessary for the formation of the metal carbonate or for the formation of the metal complex of the metallic element with the chelating agent; a step in which the aqueous alkali solution is heated to react the hydrogen carbonate ions or the like with the metal complex, thereby forming a metal carbonate; and a step in which carbon dioxide is injected into the aqueous alkali solution containing the formed metal carbonate to thereby form hydrogen carbonate ions or the like. The raw material is supplied and the metal complex formation step, the metal carbonate formation step, and the step for forming hydrogen carbonate ions or the like are performed. The method for carbon dioxide fixation further includes a step in which the unnecessary ions are removed.

This invention discloses a high-temperature resistant and calcium-sensitive cementing fluid loss reducing agent. This fluid loss reducing agent is a copolymer with a micro-crosslinked stable structure, prepared by free radical copolymerization of sulfonic acid-containing olefin monomers, tertiary amine ester functional monomers, amide-containing olefin monomers, cyclic functional monomers, and crosslinked monomers. The cyclic monomers lock the main chain conformation, and the crosslinked network provides spatial protection, significantly enhancing the stability of the ester side chains at the physical level. This fundamentally inhibits the formation of harmful carboxyl groups, improving the product's calcium sensitivity resistance and high-temperature resistance. This fluid loss reducing agent exhibits excellent high-temperature and strong alkali resistance, suitable for application in a wide temperature range of 80℃ to 220℃, alkaline environments with pH 8 to 12, and high calcium ion concentrations. It also shows good compatibility with cement slurry, without adversely affecting its rheological properties or thickening time. It can be widely used in cementing operations in complex formations such as deep onshore wells, high-temperature and high-pressure offshore wells, and salt-gypsum layers.

The invention relates to the field of oilfield chemistry, in particular to a low-loss oilfield blocking remover and a using method thereof. Comprising the following raw materials in parts by weight: 5-30 parts of a modified flaky ferric hydroxide material, 1-10 parts of 3-hydroxy-2-[(phosphono formyl) amino] sodium propionate, 0.5-5 parts of a surface regulating agent, 0.5-3 parts of a slow release regulator, 0.2-2 parts of a pH buffer agent, 0.5-3 parts of a biodegradable stabilizer and 47-92.3 parts of deionized water, and through interface regulation and control and structural stability design, the blockage removal treatment on the reservoir blockage is effectively realized. The system can reduce secondary damage to strata while maintaining a good permeability improvement effect, has excellent viscosity retentivity and mineralized water stability, and is outstanding in emulsification and dispersion performance, and the overall performance shows higher applicability and persistence under complex stratum conditions.

The application discloses a non-denaturing mass spectrometry detection method for determining interaction between aptamer and small molecule target, comprising a spray needle coated with conductive coating; preparation of aptamer and target molecule stock solution; first mass spectrometry analysis of mixed solution, obtaining combination ratio of both; analysis on stability of complex ion in tandem mass spectrometry mode; in the detection method, on one hand, a nano-sized electrospray emission spray needle is used, for sample solution containing high concentration of non-volatile salt, which significantly reduces adduct and ion cluster of complex formation, improves resolution and signal-to-noise ratio, and is simple in operation and small in sample consumption; on the other hand, through non-denaturing mass spectrometry detection, stoichiometric relationship of combination and stability change of aptamer before and after combination can be directly obtained; the method can quickly and accurately identify interaction between nucleic acid aptamer and target molecule, and is expected to be further applied to rapid screening and sequence optimization of nucleic acid aptamer.

The invention discloses a zinc oxide nanocrystal compound, a dispersion liquid, a film, an electronic device and a compound forming method. Wherein the zinc oxide nanocrystalline compound comprises a crystal nucleus of spherical wurtzite type zinc oxide nanocrystalline and a ligand layer covering the surface of the crystal nucleus, the ligand layer only comprises a-OH ligand, a Cl ligand, a Li ligand and an H ligand, and the zinc oxide nanocrystalline film is formed by spin-coating deposition of the nanocrystalline compound. The electronic device comprises a first electrode, a second electrode and the zinc oxide nanocrystalline film functional layer. The zinc oxide nanocrystalline compound provided by the invention is pure in surface and free of any organic ligand; by applying the novel organic ligand-free zinc oxide nanocrystal material, the nanocrystal spacing in a state of depositing into a thin film is effectively reduced, and the electron transmission conductivity is improved.

To provide a method for simply and selectively separating and recovering high-purity dysprosium from a solution containing neodymium ion and dysprosium ion whose simple separation is difficult.SOLUTION: A dysprosium complex is created by allowing a tripod-type Schiff ligand to react with neodymium ion and dysprosium ion in a mixed solvent of an aprotic polar solvent and a protic polar solvent. By fractionally precipitating the dysprosium complex, a separation condition for precipitating the dysprosium complex as a crystal in 1 step is determined, and dysprosium is separated at purity of 99% or more.SELECTED DRAWING: None

This invention provides an oilfield tracer and a method for evaluating the oil production contribution of different sections of a horizontal well, belonging to the field of oilfield development technology. The oilfield tracer provided by this invention comprises white oil, a nonionic surfactant, sodium 3-allyloxy-2-hydroxypropanesulfonate, and alcohols; wherein the rare earth metal salt forms a complex with a complexing agent and sodium 3-allyloxy-2-hydroxypropanesulfonate; the complexing agent is a mixture of glycolic acid, triethylenetetramine, and sodium dimercaptopropanesulfonate in a mass ratio of 10:(3-5):(1-2). This oilfield tracer is stable under complex formation conditions and can withstand high salinity. In evaluating the oil production contribution of different sections of a horizontal well using this oilfield tracer, different types and amounts of tracers can be selected for different sections, enabling the evaluation of the oil production contribution of each section of the horizontal well and providing a valid basis for subsequent repeated fracturing.

The present invention relates to nanostructure-bonding partner conjugates, as well as reaction mixtures, analyte detection devices, and methods for fabricating and using conjugates. [Solution] In particular, the present invention provides a method for detecting a target analyte in a sample, comprising the step of mixing the sample with a first detection conjugate and a second detection conjugate in a solution, wherein the first and second detection conjugates include metal nanostructures linked to binding partners that can specifically bind to the target analyte when it is present in the sample, forming a complex between the first detection conjugate and the analyte and the second detection conjugate, and the change in optical signal during complex formation indicates the presence of the target analyte in the sample.

A method of manufacturing a semiconductor device in a semiconductor body having a first surface and a second surface is proposed. Semiconductor device elements are formed in the semiconductor body by processing the semiconductor body at the first surface. A wiring area is formed over the first surface of the semiconductor body. The semiconductor body is attached to a carrier via the wiring area. Thereafter, ions are implanted through the second surface into the semiconductor body. The ions are ions of a doping element, or ions, which induce doping by complex formation, or ions of a heavy metal. A surface region of the semiconductor body at the second surface is irradiated with a plurality of laser pulses. Thereafter, the carrier is removed from the semiconductor body.

The invention relates to the field of oilfield chemicals, and discloses polyacrylamide suitable for oilfield reinjection sewage preparation and a preparation process thereof, the composition comprises the following components by mass: 74-78% of an acrylamide monomer, 10-20% of an initiator, 10-20% of an initiator, 10-20% of a crosslinking agent, 1-3% of a crosslinking agent, 1-3% of a crosslinking agent, 1-3% of a crosslinking agent, and the balance of water. 12%-16% of a 2-acrylamide-2-methyl propane sulfonic acid monomer; 0.1%-3% of a sulfobetaine type acrylamide monomer; 0.05% to 0.5% of a long-chain alkyl acrylamide monomer; the total addition amount of the initiator accounts for 0.1%-0.14% of the total mass of the monomers; the adding amount of the stabilizer accounts for 0.02%-0.04% of the total mass of the monomers; and the balance of deionized water. The sulfobetaine type acrylamide monomer is adopted, so that the polymer can still maintain good viscosity retention ability and solution stability under the condition of extremely high salinity, especially under the condition of a complex stratum with high content of high-valence metal ions.