96 results about "Emulsion droplet" patented technology

The invention discloses a dual-mode micro-droplet generation micro-fluidic chip and application thereof in generation of dual-emulsion droplets, and relates to the technical field of micro-fluidic chips. The method comprises the following steps: respectively injecting two different internal phase solutions from the left ends of respective iron tubes, guiding the internal phase solutions to a wedge-shaped tube orifice of a theta-shaped glass capillary tube through respective thin tubes, injecting an intermediate phase solution into a square glass tube through an intermediate phase inlet by using a No.1 needle, and injecting an external phase solution into the square glass tube through an external phase inlet by using a No.2 needle; the two internal phase solutions, the intermediate phase solution and the external phase solution converge at an inlet of the circular glass capillary tube, and after entering the circular glass capillary tube, water-in-oil-in-water double-emulsion droplets are obtained. The invention can obtain the dual-mode micro-droplet generation micro-fluidic chip and the application of the dual-mode micro-droplet generation micro-fluidic chip in generation of dual-emulsion droplets.

The invention discloses a mechanical property regulation and control method of an aromatic microcapsule, and belongs to the technical field of microcapsules. The preparation process of the microcapsule comprises the following steps: self-assembling CNC and an amino-functionalized polymer ligand on an oil-water interface to form a stable Pickering emulsion. Wall materials are deposited on the surfaces of emulsion droplets in situ, the CNC interface coverage rate is regulated and controlled by adjusting parameters such as the ligand concentration and the emulsion water phase pH value, and then a series of aromatic microcapsules with different mechanical properties are prepared. In addition, the functional microcapsules can be designed in a customized manner according to different application requirements, and good application prospects are shown.

The invention relates to a hydrophilic acrylic pressure-sensitive adhesive. The pressure-sensitive adhesive comprises an acrylic copolymer, polyvinyl alcohol or a derivative thereof, a non-reactive surfactant and a neutralizer according to the solid content of an emulsion, the acrylic copolymer is obtained by copolymerization of n-butyl acrylate, 2-ethylhexyl acrylate, 2-hydroxyethyl acrylate, acrylic acid, methyl methacrylate and a boric acid functional monomer emulsion containing a 2, 1-benzoxaborazole group. The preparation method comprises the following steps: dropwise adding a pre-emulsion, lagging dropwise adding an initiator and carrying out thermal-insulation polymerization to obtain a copolymer emulsion, adding polyvinyl alcohol or a derivative thereof at a specific temperature, neutralizing to a specified pH value, and carrying out segmented drying to form the film. The hydrophilic acrylic pressure-sensitive adhesive disclosed by the invention has both hydrophilicity and wet stability while keeping the adhesion performance.

The application provides a miniemulsion polymer, which is prepared by a miniemulsion polymerization method through controlling pre-emulsion droplet particle size by a high-pressure homogenizer, using specific auxiliary stabilizers and specific primary emulsifiers. After the miniemulsion polymer is incorporated into a base material, an engineering material with high air tightness, high water tightness, high bonding strength and excellent durability can be obtained, which is used for the durability protection or sealing of engineering structures or the bonding between different materials, and has the advantages of simple process, low cost and outstanding comprehensive performance.

The application discloses heparin sodium cream and a preparation method thereof, and belongs to the technical field of pharmaceutical preparation production. The heparin sodium cream comprises the following components: heparin sodium, propylene glycol, hydroxyphenyl ethyl ester, sodium dodecyl sulfate, glycerol monostearate and distearate, octadecanol, liquid paraffin, white vaseline and water. The preparation method comprises the following steps: preparing an oil phase and mixing an aqueous phase; taking part of the mixed aqueous phase and the oil phase to form a water-in-oil type primary emulsion; then adding the remaining mixed aqueous phase into the primary emulsion, inducing phase transition into an oil-in-water type cream under mild conditions, and obtaining the finished product after vacuum defoaming and gradient cooling. The application realizes high-efficiency emulsification under mild conditions below 80 DEG C and 3000 rpm through a controlled phase inversion emulsification process, so that the particle size (D99) of the emulsion droplets is less than 5 microns, and the problem that the active substance heparin sodium loses activity under severe conditions is solved completely, and the stability of the product is improved significantly.

This invention discloses a natural antibacterial polydopamine microcapsule and its preparation method. The shell material of the natural antibacterial polydopamine microcapsule is polydopamine, and the core material is Artemisia argyi oil. Using a soft template method with dimethyldiethoxysilane (DMDES) as the raw material, dopamine is in-situ self-polymerized on the surface of polydimethylsiloxane (PDMS) emulsion droplets solubilized with Artemisia argyi oil to prepare natural antibacterial polydopamine microcapsules encapsulating Artemisia argyi oil. The microcapsules prepared by this invention are micrometer-sized, monodisperse, and have high sphericity, with an average size of 1 μm. They exhibit high encapsulation efficiency for Artemisia argyi oil, with the highest encapsulation efficiency reaching 21.5% of the total mass of the microcapsules. This invention overcomes the disadvantages of high cost and environmental pollution caused by the large use of surfactants in classic emulsions. The preparation process is simple; it overcomes the defects of Artemisia argyi oil's volatility and instability; it has good monodispersity and certain antibacterial efficacy; and it can have a potential long-lasting antibacterial effect through the sustained release of Artemisia argyi oil. The preparation of monodisperse natural antibacterial polydopamine microcapsules has broad application potential in the industrial production of aromatic microcapsules.

The application provides a nanoemulsion and a preparation method and application thereof. The total amount of raw materials for preparing the nanoemulsion is 100%, and the raw materials include the following components: 1-40% of a composite surfactant, 1-25% of an oil phase, 1-30% of a co-surfactant, and the balance of a water phase; wherein the mass of the composite surfactant is 100%, and the composite surfactant includes the following components: 5-25% of a biological surfactant, 35-65% of a fatty alcohol polyoxyethylene ether surfactant, 1-20% of dodecyl benzene sulfonic acid and / or sodium salt thereof, 1-20% of oleic acid, and 1-5% of citric acid; the biological surfactant includes one or more than two combinations of sophorolipid, rhamnolipid, trehalose lipid, surfactin, alkyl polyglycoside, phosphatidylcholine, amino acid lipid, saponin, phospholipid, or derivatives thereof; and the average particle size of the emulsion droplets in the nanoemulsion is 1.5-5 nm. The nanoemulsion has excellent recovery performance.

This invention discloses an anionic hydroxyl silicone oil emulsion and its preparation method, relating to the field of polysiloxane emulsion technology, and belonging to patent classification number C08G77 / 04. The preparation method is as follows: Triethanolamine dodecylbenzenesulfonate salt, tridecyl alcohol polyether, and deionized water are mixed and dissolved, heated, and polydimethylsiloxane alcohol is added under high-speed shearing for homogenization. Water is added and the reaction is maintained at a constant temperature to obtain a basic emulsion. γ-(methacryloyloxy)propyltrimethoxysilane is added dropwise to react and obtain an interface-reinforced emulsion. Acrylamide, N-hydroxymethylacrylamide, etc., are pre-emulsified and then added to the interface-reinforced emulsion with ammonium persulfate under nitrogen protection. Polymerization forms a cross-linked polymer shell. After cooling, tert-butylhydroquinone and disodium ethylenediaminetetraacetate are added to obtain an antioxidant emulsion. Phenoxyethanol and octyl glycol are added to obtain the final emulsion. This method significantly improves the thermal stability of the emulsion through the synergistic effects of composite emulsification, interface reinforcement, shell protection, and antioxidant properties, making it suitable for high-temperature environments.

This invention belongs to the field of fire extinguishing materials and their preparation technology, and provides a fire extinguishing microcapsule and its microfluidic controllable preparation method. The preparation method is characterized by the following steps: (1) preparing fluids of each phase, (2) preparing a dual emulsion using a microfluidic device, (3) collecting and sealing the dual emulsion in a collection container, controlling the axis of the collection container to be horizontal, and controlling the collection container to rotate around its axis for 1 to 15 minutes, applying light to solidify the outer shell of the dual emulsion droplets while maintaining the rotation of the collection container, cleaning, and drying to obtain the final product. The fire extinguishing microcapsule prepared by this method consists of an outer shell and a liquid fluorine-containing fire extinguishing agent encapsulated within the outer shell. The outer shell of the fire extinguishing microcapsule has a uniform thickness, and the eccentricity between the outer shell and the core is less than 0.1. The method of this invention can achieve precise customization of fire extinguishing microcapsules according to the differentiated fire characteristics of different application scenarios, and can improve the concentricity between the core and the outer shell of existing fire extinguishing microcapsules.

This invention belongs to the field of microfluidic control technology and discloses a coaxial copolymer microfluidic droplet generation device and method with adjustable focusing distance. The device includes a focusing distance adjustment mechanism, a coaxial premixing module, and a flow focusing module. The coaxial premixing module achieves stable premixing of the inner and middle phase fluids through coaxial arrangement of inner and outer capillaries and a turbulence support structure within the annular gap, forming a core-shell structured preemulsion. The flow focusing module receives the outer phase input and performs hydraulic focusing and shearing on the preemulsion to generate uniform droplets. By precisely adjusting the distance between the two modules through the focusing distance adjustment mechanism, the focusing distance can be continuously changed within the range of 0.0-10.0 mm, achieving fine control over droplet size (260-650 μm) and shell thickness. This invention features a stable structure, precise adjustment, and high pressure resistance, and is suitable for W / O / W multiphase systems, solving the problems of limited adjustment parameters and invariable structure in traditional devices for emulsion droplet control.

The invention relates to the field of structural color materials, and discloses polymerization-induced phase separation total internal reflection structural color microspheres as well as a preparation method and application thereof. The method comprises the following steps: mixing an epoxy vinyl monomer, a fluorocarbon which cannot be subjected to polymerization reaction and a photoinitiator to obtain an oil-phase mixture; the epoxy vinyl monomer and the fluorocarbon are mutually soluble, and the fluorocarbon does not participate in polymerization; the preparation method comprises the following steps: adding a first surfactant and a second surfactant into water, and mixing to obtain a water-phase mixture; and mixing the oil-phase mixture and the water-phase mixture to form homogeneous-phase emulsion liquid drops, and irradiating the homogeneous-phase emulsion liquid drops under ultraviolet light to obtain the total internal reflection structural color microspheres. The total internal reflection structural color microspheres are prepared by optimizing the composition of two phases of Janus liquid drops and introducing ultraviolet light to initiate monomer polymerization and drive the monomers to be subjected to phase separation from emulsion liquid drops, the preparation process is simplified, cosolvent dissolution is not needed, and the requirements of large-scale industrial production can be met.

The present application belongs to the field of food contact coating materials, and provides a food contact grade silicone emulsion and a preparation method thereof. The silicone intermediate with a core-shell emulsion droplet structure is synergistically compounded with a methyltrimethoxysilane surface modified silica intermediate, and combined with hydrolysis and condensation pre-emulsification, high pressure homogenization, aging, defoaming, filtration and control of emulsion droplet and particle size distribution to obtain a water-based system with stable dispersion and workability. The system still maintains suitable viscosity under higher non-volatile conditions, and after film formation, it has the performances of adhesion, wear resistance, heat resistance and stable release, solving the problem that the existing system cannot simultaneously consider high solid content, low viscosity, processing adaptability, high adhesion, wear resistance and film forming performance, and high heat resistance, stability and low release force. The present application has application value in constructing a silicone release layer on the surface of paper base material and plastic film base material.

This disclosure provides a method for detecting an analyte in a sample, wherein the sample is introduced into an analytical chamber along with emulsion droplets or gel beads. In another aspect, this disclosure provides designs for formulating emulsion droplets or gel beads such that they can be used for the large-scale parallel detection of analytes. Formulations containing specific combinations of fluorescent particles allow for the optical determination of the identity of each fluorescent particle. These combinations are based on the particle fluorescence emission wavelength, fluorescence excitation wavelength, and particle count.

The invention belongs to the technical field of pharmaceutical preparations, and particularly relates to a riptacaine cream and a preparation process thereof. A part of water, polyoxyethylene hydrogenated castor oil and a part of carbomer are preferentially mixed to form a water phase, so that the operation temperature in the cream production process can be reduced, hydrogen bonds can be further formed between polyoxyethylene hydrogenated castor oil and carbomer molecules, and meanwhile, carbomer forms a loose three-dimensional structure. When an oil phase and a water phase are mixed, homogenized and emulsified, a hydrogen bond formed between polyoxyethylene hydrogenated castor oil and carbomer molecules can buffer pulling of a homogenized shear force on a stretched carbomer molecular chain to prevent the carbomer molecular chain from being broken. Secondly, after emulsification, a composite interfacial film of a surfactant and macromolecules can be formed on the surface of an oil phase, so that damage to emulsion droplets during collision of the oil droplets can be effectively resisted, and coalescence and flocculation are reduced. The ripropylene dicaine cream prepared by the invention is milk white paste with fine texture, the particle size range is 1-2 microns, and the character, the particle size and the viscosity are basically unchanged after a low-temperature cycle test.

The invention relates to the technical field of deep processing of egg products, in particular to egg white peptide / polyphenol / metal ion ternary synergistically assembled composite particles and application of the egg white peptide / polyphenol / metal ion ternary synergistically assembled composite particles in stable emulsions.The egg white peptide / polyphenol / metal ion ternary synergistically assembled composite particles are prepared by pre-assembling metal ions and polyphenol to form a metal-phenolic network and synergistically assembling the metal-phenolic network and egg white peptide through an anti-solvent coprecipitation method. The egg white peptide / polyphenol / metal ion ternary synergistic assembly composite particles are formed. Compared with unassembled egg white peptide, the colloidal property of the composite particle is regulated and controlled, the absolute value of surface charge is reduced, the interface hydrophobicity is enhanced, and the egg white peptide molecule conformation is promoted to be converted into an ordered secondary structure mainly comprising beta-folding. The composite particle can be used as a novel stabilizer for constructing an emulsion, and the obtained emulsion is uniform in droplet size, good in dispersity and still stable under the environmental stress conditions of heating, freezing and thawing, different pH values, salt ion strength and the like. The invention provides a new technical approach for the application of the egg white source active peptide in emulsion and functional food.

The invention discloses heparin sodium cream and a preparation method thereof, and belongs to the technical field of pharmaceutical preparation production. The heparin sodium cream is prepared from the following components: heparin sodium, propylene glycol, ethylparaben, lauryl sodium sulfate, glyceryl monostearate and glyceryl distearate, octadecanol, liquid paraffin, white vaseline and water. The preparation method comprises the following steps: preparing an oil phase and a mixed water phase; emulsifying a part of the mixed water phase and the oil phase to form a water-in-oil primary emulsion; adding the residual mixed water phase into the primary emulsion, inducing phase transformation into oil-in-water emulsifiable paste under a mild condition, and performing vacuum defoaming and gradient cooling to obtain a finished product. Through a controlled phase inversion emulsification process, efficient emulsification is realized under mild conditions of 80 DEG C or below and 3000 rpm or below, the emulsion droplet particle size (D99) is less than 5 [mu] m, the problem that the active substance heparin sodium loses activity under severe conditions is thoroughly solved, and the product stability is significantly improved.

The application provides a method for preparing hollow non-spherical micro-nanoparticles based on a coaxial micro-reactor, and belongs to the field of droplet microfluidics and polymer materials. A three-channel coaxial micro-reactor is designed to generate a water-in-oil-in-water (W / O / W) double emulsion. The innermost phase is an alkaline electrolyte aqueous solution, the intermediate oil phase is a polymer solution, and the outermost phase is a surfactant aqueous solution. With the volatilization of the intermediate phase solvent, the double emulsion droplets form hollow non-spherical micro-nanoparticles. The double emulsion is placed in a collection liquid with different electrolyte concentrations, water molecules in the collection liquid penetrate into the innermost phase under the action of osmotic pressure, increase the size of the inner droplets of the double emulsion, and then change the morphology of the outer droplets, so that the non-spherical structure required is presented, and the size of the hollow micro-nanoparticles is controllable. The coaxial micro-channel reactor used in the application adopts a flow focusing structure, which is used for controllable generation of double emulsion to prepare multi-chamber hollow non-spherical micro-nanoparticles.

The invention provides a porous ceramic material prepared based on a high internal phase emulsion template method and a preparation method and application thereof. The porous ceramic material is prepared from the following raw materials: a high internal phase emulsion and an adhesive, the high internal phase emulsion comprises a continuous phase and a dispersed phase; the continuous phase is a ceramic precursor aqueous solution; the dispersion phase is an emulsifier oil phase solution; the volume fraction of the dispersion phase in the high internal phase emulsion is 74-95%. According to the preparation method, the size of emulsion droplets is accurately regulated and controlled through a high internal phase emulsion template method, and accurate control over the pore diameter of the ceramic material is achieved, so that the porous ceramic material has high porosity and high strength, and is stable in structure, controllable in average pore diameter and wide in application range.

The present invention provides methods for analyzing large nucleic acids including chromosomes and chromosomal fragments. In one aspect, the present invention provides a method of nucleic acid analysis comprising the steps of (a) obtaining a sample of nucleic acid comprising at least one chromosome or fragment greater than about 1,000 base pairs in length and containing a target region; (b) creating an emulsion in which each drop of the emulsion contains an average of between about 0-2, 0-1.75, 0-1.5, 0-1.0, 0-0.75, 0-0.5, or fewer chromosomes or fragments of step (a), (c) performing emulsion PCR, (d) quantifying the number of emulsion droplets containing amplified nucleic acid from the target region; (e) calculating the ratio of droplets containing amplified nucleic acid from the target region to total droplets; and (f) comparing the ratio of step (e) to a reference ratio representing a known genotype.

The present application provides a scale inhibitor which can be attached and solidified in stratum and its preparation method. The scale inhibitor is composed of a scale inhibition system and water absorbent resin, is in emulsion state and can be attached and solidified in stratum. The whole emulsion droplet can be deformed according to the pore throat of stratum and is not easy to be blocked, and can enter into the production layer. The scale inhibitor has low viscosity on the ground, and after staying in the porous medium, gradually thickens under the action of crosslinking agent, can stay in the stratum for a long time, and slowly degrades after meeting divalent cations, the scale inhibitor is slowly released, the scale prevention period is long, the shortcomings of traditional adsorption type or precipitation squeeze process are overcome, and long-term scale prevention is realized after the measures on the wellbore and stratum. The superfine silicon dioxide particles suspended in the water absorbent resin can guarantee good conductivity between resin particles, the bactericide endows the product with the characteristics of bacteriostasis and sterilization, can effectively prevent the rapid degradation of the resin and prolong the release period of the scale inhibitor.

The invention relates to a preparation method of a single-layer functional particle layer based on micro-fluidic-ink-jet printing and the single-layer functional particle layer, and solves the problems that in the existing ink-jet printing process, ink containing preformed particles is used for preparing the functional particle layer, and a single-layer structure functional layer with closely-packed particles is difficult to prepare; according to the method, firstly, water-in-oil emulsion is prepared through a micro-fluidic chip, secondly, emulsion liquid drops are output through ink-jet printing equipment and deposited on a substrate, and finally, a single-layer functional particle layer is formed through evaporation and self-assembly. The method can be used for preparing single-layer functional particles and functional layers which are uniform in particle size and orderly arranged on a large scale.

The invention discloses a preparation method of phenylethyl resorcinol / sodium alginate emulsion gel beads as well as a product and application of the phenylethyl resorcinol / sodium alginate emulsion gel beads. The method comprises the following steps: firstly, dissolving phenethyl resorcinol in an oil phase, mixing with a water phase containing sodium alginate and a thickening agent, and homogenizing to form an oil-in-water emulsion; then, through an extrusion titration process, the emulsion is dropped into a calcium salt solution to be subjected to an ionic cross-linking reaction, and emulsion gel beads are formed through curing. According to the invention, the problems of poor water solubility, strong irritation and the like of phenylethyl resorcinol are effectively solved by utilizing an emulsion filled gel network structure, and meanwhile, the defect of unstable thermodynamics of the traditional emulsion is overcome. The prepared gel beads are high in sphericity degree, good in mechanical stability and good in mechanical property, can play a dual whitening effect by inhibiting tyrosinase activity and down-regulating protein expression of tyrosinase, and has a wide application prospect in the field of functional cosmetics.

This invention discloses a method for preparing a novel (O1+W) / O2 emulsion using a compound of two surfactants in the field of chemical technology. The emulsion system comprises a fluorocarbon oil phase, an aqueous phase, and a hydrocarbon oil phase. By compounding two types of surfactants with different hydrophilic and hydrophobic properties, using an aqueous solution of fluorocarbon oil and FS-30 as the inner phase, and a Span series surfactant dissolved in ethoxylated trimethylolpropane triacrylate as the outer phase, the two are mixed and shaken to spontaneously form a novel (O1+W) / O2 emulsion with ETPTA as the continuous phase (O2) and containing fluorocarbon oil (O1) and water (W) internally. The emulsion droplets consist of two microregions with significantly different hydrophilic and hydrophobic properties. The preparation process is simple, inexpensive, and easy to apply industrially, providing a new technical pathway for the application of amphiphilic Janus materials in interface science, drug delivery, catalysis, and functional materials.

The invention discloses a drug inspection reagent mixing device, and relates to the technical field of reagent mixing, the drug inspection reagent mixing device comprises a bottom plate and a U-shaped support plate arranged on the bottom plate, the U-shaped support plate is provided with at least two groups of transparent test tubes, the outer sides of the transparent test tubes are provided with output holes, and the U-shaped support plate is provided with a moving plate and a threaded adjusting rod; a blocking column and a blocking plate are fixedly connected to the upper portion of the moving plate, the blocking plate can be driven to move by pressing the moving plate or rotating the threaded adjusting rod so as to adjust the output quantity of the output hole, and a mixing container is arranged below the U-shaped supporting plate; the rotating rod is connected with the mixing container through the first pulling rope, the rotating rod is driven to control the rotating angle and speed, the shaking amplitude of the mixing container can be adjusted and controlled through the first pulling rope, the mixing container can adapt to the characteristics of the drug inspection reagent of an emulsion type solvent and a suspension type drug solvent, emulsion droplets are evenly dispersed on the premise that the membrane structure is not damaged, and the stability of the mixing container is improved. The emulsion stability is maintained.

The invention discloses hydrolyzed collagen freeze-dried microspheres as well as a preparation method and application thereof. The method comprises; dissolving hydrolyzed collagen and a freeze-drying protective agent in a phosphate buffer solution to obtain an inner water phase; mixing caprylic / capric triglyceride and polyglycerol-10-oleate in a molten state to obtain an intermediate oil phase; dissolving sodium alginate, sodium hyaluronate and hyaluronic acid in water to obtain an outer water phase; generating monodisperse W / O / W emulsion drops from the inner water phase, the intermediate oil phase and the outer water phase; dropwise adding the monodisperse W / O / W emulsion into the calcium ion-containing solution to obtain a first material body; carrying out molding treatment on the first material body in a mold to obtain microspheres; the freeze-drying treatment of the microspheres comprises the following steps: pre-freezing the microspheres; carrying out deep freezing treatment on the microspheres; and carrying out sublimation drying on the microspheres to obtain the hydrolyzed collagen freeze-dried microspheres. The hydrolyzed collagen freeze-dried microspheres provided by the invention have the characteristics of rapid redissolution and long-acting release and repair, and have excellent stability and permeability.

The invention belongs to the technical field of microfluid control, and discloses a coaxial copolymerization microfluidic droplet generation device and method with an adjustable focusing distance. The device comprises a focusing distance adjusting mechanism, a coaxial premixing module and a flow focusing module. The coaxial premixing module is used for realizing stable premixing of an inner phase fluid and an intermediate phase fluid through coaxial arrangement of inner and outer capillary tubes and a turbulent flow supporting structure in an annular space, so as to form a core-shell structure pre-emulsion. And the flow focusing module performs hydraulic focusing and shearing on the pre-emulsion through external phase input to generate uniform liquid drops. The distance between the two modules is accurately adjusted through the focusing distance adjusting mechanism, the focusing distance can be continuously changed within the range of 0.0-10.0 mm, and fine regulation and control of the liquid drop size of 260-650 [mu] m and the shell thickness are achieved. The device is stable in structure, accurate in adjustment, resistant to high pressure and suitable for a W / O / W multiphase system, and the problems that a traditional device is limited in adjustment parameter type for emulsion drop control and invariable in structure are solved.

The invention discloses a preparation method of hyaluronic acid microspheres with high uniformity and high cross-linking density, W / O / W type double-emulsion droplets with uniform size are prepared through a micro-fluidic chip, in the preparation process of the double-emulsion droplets, a mixed solution of modified hyaluronic acid and a photoinitiator is used as an inner water phase solution, an oil phase solution is a water-insoluble solution, and the inner water phase solution and the oil phase solution are used as an outer water phase solution. A solution containing a higher salt concentration is used as an external aqueous phase solution. The internal water phase is subjected to lossless concentration by utilizing the osmotic pressure of the internal and external phases, water molecules in the internal water phase solution migrate to the external water phase under the influence of the osmotic pressure, and the concentration of the methacrylated hyaluronic acid in the double-emulsion droplets is gradually increased, so that the concentration of the hyaluronic acid microspheres can be increased to 8.4 wt% or above; the problem that in the prior art, a high-concentration hyaluronic acid microsphere solution cannot be directly used for preparing the microspheres through a microfluidic technology is solved, and the crosslinking density of the hyaluronic acid microspheres can be further improved and the size and the crosslinking density can be accurately controlled due to the improvement of the concentration.

The application discloses a preparation method of a hydrophobic H-SiO2@PNIPAM hybrid microgel stabilized phase inversion type Pickering emulsion and application thereof, and relates to the technical field of nanometer material preparation and biological culture. The application prepares the surface hydrophobic H-SiO2@PNIPAM hybrid microgel particles through a precipitation polymerization method and a sol-gel reaction, then uses the hybrid microgel as an emulsifier to stabilize a W / O type Pickering emulsion, can encapsulate the obligate anaerobe bifidobacterium in the inner part of the emulsion droplets, and realizes efficient culture. The hybrid microgel prepared in the application has strong hydrophobicity, and meanwhile, the temperature sensitivity of the PNIPAM core is reserved, so that the Pickering emulsion stabilized by the hybrid microgel also has temperature sensitivity, and the emulsion can be phase-inverted only by temperature change, so that the inner phase bacteria are released, and rapid separation is realized. Compared with the conventional anaerobe culture method, the method disclosed by the application can realize efficient culture and rapid separation of the bacteria after culture, and has a good application prospect.

The invention discloses an emulsifier and a colostrum preparation method thereof, and belongs to the technical field of microfluidic emulsification, the emulsifier comprises an upper cover and a valve seat, the upper cover and the valve seat are fixedly connected to form an emulsifier main body; the micro-pore plate is arranged between the upper cover and the valve seat, and a micro-pore channel is formed in the micro-pore plate and is used for realizing focusing emulsification of a dispersed phase and a mobile phase; the sealing element is arranged between the upper cover and the valve seat and is used for realizing axial and radial sealing; the mobile phase inlet is formed in the side part of the valve seat or the upper cover and is used for introducing a mobile phase; the dispersed phase inlet is formed in the top of the upper cover or the valve seat and is used for introducing a dispersed phase; the mixed phase outlet is used for discharging emulsified mixed liquid; and the emulsifier main body is configured to realize continuous regulation and control on the particle size of the emulsified liquid drops under the condition of not replacing the micro-pore plate by regulating the flow velocity of the mobile phase and the dispersed phase. According to the present invention, on the premise of not replacing the microporous plate, the continuous regulation and control effect on the emulsion droplet particle size can be achieved by adjusting the flow rates of the mobile phase and the dispersed phase.

The invention discloses a preparation method of a multifunctional concrete admixture. The preparation method of the concrete multifunctional admixture comprises the following steps: firstly, mixing an oil phase system A formed by PVC and an organic solvent, Span-80, ettringite and calcium carbonate to obtain a solution B, and then emulsifying the solution B with a sodium thiosulfate solution to obtain an emulsion D; secondly, dropwise adding the emulsion D into a PVA emulsion, stirring to form a double emulsion E, stirring, centrifuging, washing, precipitating and drying to obtain microspheres F; and finally, mixing the microspheres F with a sodium sulfate solution, PVA and nano ferroferric oxide to obtain a turbid liquid I, mixing PVC with a plasticizer, a solvent and Span-80 to obtain an oil phase system G, dropwise adding an emulsion J emulsified by I and G into a PVA emulsion, and carrying out after-treatment to obtain the multifunctional concrete admixture. The admixture prepared by the method disclosed by the invention is a microsphere-in-microsphere structure body, so that the integration of concrete early strength and crack repair functions is realized, and the problem of concrete shrinkage cracks caused by early strength can be quickly solved.