42 results about "Litsea cubeba" patented technology

The invention discloses a traditional Chinese medicine composition for treating type 2 diabetes and a preparation method and application thereof. The traditional Chinese medicine composition is prepared from, by weight, 11-15 parts of radix acanthopanacis semticosi, 5-15 parts of male silkworm moth, 7-17 parts of white ginseng, 5-11 parts of rhizoma polygoni cuspidati, 15-25 parts of rhizoma coptidis, 1-5 parts of cortex cinnamomi, 11-15 parts of fructus ligustri lucidi, 11-15 parts of common fenugreek seed, 7-17 parts of lithocarpus litseifolius, 3-7 parts of raw radix astragali and 1-3 parts of wild lucid ganoderma. , 3-9 parts of tribulus terrestris and 11-17 parts of nigella nigra seeds. The traditional Chinese medicine composition has the effect of balancing and stabilizing blood sugar, is short in treatment course, quick in effect and free of toxic and side effects, and has an excellent curative effect on treating diabetic complications.

This invention discloses a Litsea cubeba extract, its preparation method, and its applications, relating to the field of pharmaceutical compound technology. The Litsea cubeba extract has the structural formula shown in Formula I: (Formula I). ​​This Litsea cubeba extract is isolated from the Litsea cubeba plant. This novel compound can significantly inhibit NO production in an LPS-induced RAW264.7 cell inflammation model; alleviate the immune-inflammatory response in an in vitro 3D skin model of atopic dermatitis; inhibit epidermal thickening and repair skin barrier function; and reduce the inflammatory response by inhibiting the PI3K / Akt signaling pathway. It has clinical application potential in the treatment of atopic dermatitis and can be used to prepare anti-atopic dermatitis drugs, showing promising application prospects.

This invention discloses a rapid propagation method for Litsea cubeba tissue culture that effectively reduces browning and improves rooting rate. The method selects healthy, young stem segments of Litsea cubeba as explants, sterilizes them, and inoculates them into a primary induction medium to induce initial bud formation. These are then inoculated into a subculture proliferation medium to obtain subculture clusters of buds. Single buds are then cut and inoculated into a rooting medium for dark culture to induce rooting. Finally, the seedlings are hardened off and transplanted to obtain tissue culture seedlings ready for nursery. This invention solves the problems of low subculture proliferation coefficient and severe browning by optimizing the explant sterilization measures, the primary induction medium, the subculture proliferation medium, and the rooting medium formulation, reducing the explant browning rate to below 5.0%. Simultaneously, the subculture yields a large number of clustered buds in a short time with a high proliferation coefficient; the tissue culture seedlings have more than four roots, resulting in a high rooting rate; the transplant survival rate is high, and the seedling quality is good. This reduces the industrial-scale production cost of Litsea cubeba tissue culture seedlings and provides technical support for large-scale seedling production and artificial cultivation of Litsea cubeba.

This invention discloses an antioxidant active peptide derived from Litsea cubeba, its preparation method, and its application, relating to the field of biotechnology. The amino acid sequence of this Litsea cubeba-derived antioxidant active peptide is NPYYFWDLSF. The preparation method of this Litsea cubeba-derived antioxidant active peptide includes the following steps: S1, extracting Litsea cubeba protein from Litsea cubeba seed cake; S2, enzymatically hydrolyzing the Litsea cubeba protein using acidic protease to obtain the enzymatic hydrolysis product; S3, separating the enzymatic hydrolysis product by ultrafiltration and collecting the ultrafiltration fraction; S4, screening candidate peptides with high antioxidant activity from the peptide fragments of the ultrafiltration fraction; the application of the Litsea cubeba-derived antioxidant active peptide in the preparation of drugs or functional foods for the prevention or treatment of oxidative stress-related diseases; the NPYYFWDLSF peptide obtained by the preparation method of this application can alleviate oxidative stress damage through the PI3K-AKT signaling pathway; this application provides a theoretical basis for the high-value utilization and potential application of Litsea cubeba by-products.

The invention provides compound essential oil with an anti-wrinkle effect and a preparation method and application of the compound essential oil, and belongs to the technical field of skin care product preparation. 5%-20% of frankincense essential oil; 5%-20% of sandalwood essential oil; 5%-15% of litsea cubeba essential oil; 10%-20% of myrrh essential oil; the compound essential oil is prepared through the processes of step-by-step constant-temperature stirring, nitrogen protection, dark curing and filtering and can be applied to skin care products such as essence oil, face cream, emulsion, eye cream or massage oil, and the adding amount is 1%-3%. The anti-wrinkle and skin-brightening essential oil achieves the effects of synergistically resisting oxidation, promoting collagen metabolism and repairing skin barrier of all the components, the anti-wrinkle and skin-brightening comprehensive effect is prominent, the preparation process is matched with the characteristics of active components of the essential oil, component loss is effectively reduced, the product stability is improved, the components are natural and mild, and the anti-wrinkle and skin-brightening essential oil conforms to relevant provisions of Cosmetic Safety Technical Specifications, is high in safety, and is suitable for industrial production. The mask is suitable for long-term use of various skins.

The invention relates to the technical field of fructus amomi planting, and discloses a ditching device for fructus amomi planting, which comprises a vehicle body, a lifting mechanism is arranged at the lower end of the vehicle body, a ditching mechanism is arranged at the lower end of the lifting mechanism, a soil discharging mechanism is arranged at the rear end of the ditching mechanism, and a blocking assembly is arranged at the discharging end of the soil discharging mechanism and is used for preventing soil from splashing; a seeding mechanism is arranged at the lower end of the vehicle body, and an opening and closing mechanism is arranged in the upper end of the seeding mechanism and used for opening and closing the interior of the seeding mechanism; a downward pressing assembly is arranged at the rear end of the lifting mechanism and used for providing power for the opening and closing mechanism. The lifting mechanism is used for driving the ditching mechanism to move downwards, so that the ditching mechanism is attached to soil, then the soil can be ditched under the action of the ditching mechanism, manual ditching is not needed in the ditching process, and therefore the working efficiency is improved; and meanwhile, the ditching depth can be effectively ensured under the cooperation of the lifting mechanism.

The invention discloses a pungent litse fruit essence for beverage and a preparation method thereof, and belongs to the technical field of beverage additives, the pungent litse fruit essence is prepared from the following raw materials in parts by mass: 0.1 part of citronellal, 0.1 part of linalool, 0.05 part of geraniol, 0.1 part of citral, 3 parts of lemon terpenoid removal liquid, 10 parts of pungent litse fruit water-soluble extract, 5 parts of pungent litse fruit fermentation extract, 61.65 parts of ethanol, 10 parts of purified water and 10 parts of propylene glycol. The citronellal, the linalool, the geraniol and the citral are used for providing head fragrance, the lemon terpene-removing liquid is used for providing soft fragrance, the pungent litse fruit water-soluble extract is used for providing main fragrance, the pungent litse fruit fermentation extract is used for providing composite fragrance, so that the fragrance of the pungent litse fruit essence is compounded and rich, and meanwhile, the pungent litse fruit extract is obtained by an ethanol water extraction method, has good water solubility, and can be used for preparing the pungent litse fruit essence. The beverage can be applied to beverages and has unique flavor.

The application discloses a rhizosphere phosphorus-fixing and phosphorus-dissolving microbial interaction regulation method and belongs to the field of microbial agents. 2+ and Mg 2+ bridge the two-way metabolite closed-loop mechanism of Bacillus megaterium exopolysaccharide, ferment by two-step method respectively and mix in an optimized ratio, prepare a synergistic microbial agent by compounding biochar and vermiculite into a heterogeneous carrier at a mass ratio of (2-4):(1-3), and apply the synergistic microbial agent in a circular ditch during the afforestation period of Litsea cubeba seedlings. The application breaks through the traditional cognition that functional antagonistic microbial groups cannot coexist, and the phosphorus accumulation amount of Litsea cubeba plants is increased by 28% compared with single phosphorus-dissolving microbial agent treatment.

The application provides a compound composition for preventing and treating motion sickness and a preparation method thereof, the compound composition comprises the following raw materials in parts by weight: dimenhydrinate 2-6 parts, dextroamphetamine 0.2-0.6 parts, ginger 20-50 parts, dried tangerine or orange peel 10-20 parts, privet fruit 8-15 parts, tribulus terrestris 8-15 parts, rhizoma alocasia 5-12 parts, pachystachys jamaicensis 8-15 parts, daya flower 5-10 parts, litsea cubeba 3-8 parts, kaempferia 3-8 parts, ipomoea alba 5-10 parts, menthol 0.5-3 parts; the compound composition of the application realizes the enhancement of the anti-motion effect and the offset of the side effects, and especially significantly reduces the drowsiness caused by dimenhydrinate and the potential risk of excessive nerve excitement of dextroamphetamine.

This invention discloses a Litsea cubeba essential oil preparation, which is prepared by mixing a 1% (w / w) chitosan quaternary ammonium salt solution with a Litsea cubeba essential oil ethanol mixture at a volume ratio of 4:1; the volume ratio of Litsea cubeba essential oil to anhydrous ethanol in the Litsea cubeba essential oil ethanol mixture is 2:3. The Litsea cubeba essential oil preparation prepared by this invention exhibits good dispersibility and stability, achieving 100% short-term and long-term antibacterial effect against Aspergillus flavus. This is significantly superior to using Litsea cubeba essential oil or chitosan quaternary ammonium salt alone, as well as other chitosan quaternary ammonium salt-Litsea cubeba essential oil preparations, and even more superior to commonly available antifungal agents. Its preparation method is simple, non-toxic, harmless, and does not pollute the environment. It has low production costs and can be used to prevent or inhibit the growth of Aspergillus flavus, reducing its damage to wooden household products.

The application provides a cultivation management method for promoting growth of amomum villosum, relates to the technical field of amomum villosum planting, and adopts composted manure, fermented material of vigna unguiculata, biochar, completely gelatinized starch, conidia of lecanimigma verrucosum, superphosphate, urea and the like to prepare a solid fertilizer for fertilization management in spring and autumn. The application overcomes the shortcomings of the prior art, effectively guarantees the growth of amomum villosum stems and further promotes early flowering and fruiting of amomum villosum, and comprehensively improves the economic value of artificial planting of amomum villosum.

The invention provides a litsea cubeba autumn cutting propagation method, which comprises the following steps: disinfecting a cutting medium, and placing the cutting medium in a seedling raising bed; in September to October, litsea cubeba branches are collected and cut into sections, and litsea cubeba cutting slips are obtained; the litsea cubeba cutting slips are soaked in a diluent of thiophanate-methyl wettable powder, after standing is conducted, upper cuts are coated with a callus smearing agent, and after bundling is conducted, the cutting slips subjected to browning prevention treatment are obtained; mixing a rooting agent solution with new yellow mud, uniformly coating the base part of the cutting slip subjected to browning prevention treatment, and standing to obtain the cutting slip subjected to hormone treatment; after clear water is sprayed to the substrate, the cutting slips subjected to hormone treatment are obliquely inserted into the cutting substrate, and then cutting management is conducted. According to the litsea cubeba autumn cutting propagation method, by adding the step of preventing branches from browning and performing management after cutting, germination and growth of stems and buds of cutting slips are continuously promoted, the rooting rate of cutting propagation is increased, the litsea cubeba cutting time range is widened, operation is easy and convenient, and cost is low.

This invention provides a traditional Chinese medicine composition for treating ulcerative colitis, belonging to the field of traditional Chinese medicine pharmaceutical technology. The composition is obtained through rational formulation based on the principles of syndrome differentiation and treatment in traditional Chinese medicine, specifically composed of Forsythia suspensa, Lilium brownii, Lysimachia christinae, Sesamum indicum, Prickly ash, Pueraria lobata, Centella asiatica, Viola yedoensis, Houttuynia cordata, Portulaca oleracea, Litsea cubeba, Cirsium japonicum, Schizonepeta tenuifolia, Morus alba root, Lophatherum gracile, Lygodium japonicum, and Capsella bursa-pastoris. This invention's composition exhibits significant anti-inflammatory and de-inflammatory effects, and can repair and improve the integrity of colonic tissue structure, enhance intestinal barrier function, and achieve both symptomatic and root-cause treatment of ulcerative colitis. Simultaneously, this invention's composition has a high efficacy and cure rate in treating ulcerative colitis, and significantly alleviates symptoms such as diarrhea, bloody stools, abdominal pain, abdominal distension, anal burning, and tenesmus, greatly reducing patient suffering.

This invention provides a traditional Chinese medicine compound preparation and its application in the preparation of drugs for treating cardiovascular and cerebrovascular diseases, belonging to the field of traditional Chinese medicine technology. The traditional Chinese medicine compound preparation comprises the following raw materials in parts by weight: 10-20 parts bee pollen, 8-15 parts curcumin, 5-10 parts γ-aminobutyric acid, 5-12 parts Litsea cubeba leaf, 10-20 parts hawthorn, 5-10 parts Lindera strychnifolia leaf, 10-20 parts kudzu root, 5-12 parts ginseng, 8-15 parts Polygonatum sibiricum, 6-12 parts Sophora japonica flower bud, 3-8 parts tangerine peel, and 1-5 parts L-cysteine. This invention significantly improves the water solubility, stability, and bioavailability of curcumin by constructing a chlorophyll zinc / LDH nanosheet composite carrier to load curcumin. A compound preparation of traditional Chinese medicine was prepared by combining a complex loaded with curcumin with various food and medicine homologous ingredients. This traditional Chinese medicine compound preparation can significantly reduce whole blood viscosity, plasma viscosity and platelet aggregation rate in rats with acute blood stasis model, has anti-thrombotic effect, and significantly promotes the recovery of neurological function in rats with focal cerebral ischemia and reduces the volume of cerebral infarction.

The invention belongs to the technical field of personal care products, and particularly relates to a bacteriostatic antioxidant moisturizing composition, a preparation method thereof and application of the bacteriostatic antioxidant moisturizing composition in skin care products. The bacteriostatic antioxidant moisturizing composition provided by the invention is prepared from 0.8 to 3.5 percent of litsea cubeba essential oil, 0.5 to 3 percent of camphor tree essential oil, 8 to 22 percent of camellia oleosa seed oil, 4 to 9 percent of glycerol, 0.2 to 0.6 percent of sodium hyaluronate, 2 to 6 percent of wool fat, 1.5 to 3.5 percent of emulsifier, 0.5 to 2 percent of panthenol and the balance of water. The litsea cubeba essential oil, the camphor tree essential oil and the camellia oleosa seed oil are used as core functional raw materials, so that the essential oil has the triple core effects of comprehensive bacteriostasis, high-efficiency oxidation resistance and long-acting moisturizing; the core components of the formula are natural components extracted from plants, so that the hand cream is safe and low in sensitivity, and is suitable for daily hand care, dry and damaged hand repair and hand bacteriostasis protection in special scenes.

The application provides a negative oxygen ion spraying liquid prepared by using tropical rainforest vegetation resources, a preparation method and application thereof, and relates to the field of negative oxygen ions. The spraying liquid comprises the following raw materials in parts by weight: 55-60 parts of deionized water, 38-42 parts of total pine needle, cinnamomum longepaniculatum leaf and litsea cubeba seed extract, 0.5-1 parts of polyvinyl alcohol, and 0.1-0.5 parts of an additive. The spraying liquid can simulate the release of negative oxygen ions by vegetation, improve the concentration of negative oxygen ions in a micro space, and improve the air quality in the micro space, and is green, environmentally friendly and pollution-free.

The application discloses a cytochrome P450 enzyme mutant and a method for catalytically synthesizing betulinic acid, and relates to the technical fields of genetic engineering and biological enzyme catalysis.The reported RoCYP01 from Litsea cubeba is used as a mutation template, site-directed mutation is carried out, the substrate channel for the entry and exit of the enzyme catalytic center of a substrate and a product is changed, and a mutant with improved catalytic efficiency for the synthesis of betulinic acid from betulonic acid is obtained.Under the same catalytic conditions, the catalytic activity of the mutant is increased by 198% compared with the wild type, which has important guiding significance for the construction of a betulinic acid yeast engineering strain.

The invention discloses a method for extracting polysaccharide from litsea cubeba distillation residues by using subcritical water, and belongs to the technical field of plant polysaccharide extraction. The method comprises the following steps: drying and crushing residues obtained after steam distillation of litsea cubeba to obtain litsea cubeba powder; adding litsea cubeba powder and deionized water into a reaction kettle, and carrying out subcritical water extraction to obtain an extracting solution; performing filtration, centrifugal separation and vacuum concentration on the extracting solution to obtain a concentrated solution; adding ethanol into the concentrated solution, standing at low temperature, collecting precipitate, and drying to obtain polysaccharide powder. According to the method, water is used as a unique extraction medium, no organic solvent participates in the whole process, and the problems of solvent residue, volatilization and pollution caused by a traditional solvent extraction method are fundamentally avoided. The subcritical water shows excellent dissolving capacity for active ingredients such as polysaccharide in litsea cubeba residues, the extraction rate of the subcritical water is remarkably higher than that of a conventional hot water extraction method, and efficient and high-valued conversion of waste resources is achieved.

The invention relates to application of a qi-regulating and blood-activating preparation in a medicine for preventing or treating altitude stress, and belongs to the technical field of medicine application. The qi-regulating and blood-activating preparation is prepared by extracting and processing fructus cinnamoii, borneol, ligusticum wallichii and allium macrostemon. Experimental research shows that the qi-regulating and blood-activating preparation can effectively improve plateau hypoxia athletic ability and heart functions, can effectively improve plateau hypoxia lung tissue inflammation infiltration, reduces alveolar wall thickness, reduces lung tissue fibrosis, and reduces myocardial cell LDH release, thereby achieving the effect of effectively preventing and treating acute altitude stress.

The present application relates to the technical field of fragrance products, in particular to a compound oil of woody plants wet incense and its preparation and preservation method. The compound oil of woody plants wet incense comprises a fokienia hodginsii incense carrier, and the fokienia hodginsii incense carrier is saturated with the compound oil of woody plants. The raw materials of the compound oil of woody plants include Chinese oil tree oil, Chinese fir oil and fokienia hodginsii oil. The compound oil of woody plants wet incense initiates the "wet" incense product form, and the incense is in an oil-containing state before burning through the saturated adsorption of the carrier. The Chinese oil tree oil, Chinese fir oil and fokienia hodginsii oil in the oil can produce a synergistic effect when burning, and efficiently neutralize and mask the tobacco odor in the environment.

The utility model discloses a sampling system for litsea cubeba rhizosphere microorganism monitoring, which comprises a sampling vehicle and a sampling device, wherein moving wheels are arranged at the bottom of the sampling vehicle; the storage box is arranged on the sampling vehicle, the storage box is provided with a storage drawer capable of being horizontally pulled, and a tool cavity used for storing sampling tools is defined in the storage drawer; the storage box is arranged on the sampling vehicle, the storage box comprises a box body and a box cover arranged at the top of the box body, a cooling cavity and a first sample cavity are arranged in the box body at intervals, a refrigerant is arranged in the cooling cavity, and a plurality of first sample bottles are placed in the first sample cavity. The sampling system for litsea cubeba rhizosphere microorganism monitoring is convenient to carry and transfer, and samples can be stored at low temperature without electricity.

This invention discloses a mixed-culture fermented Polygonatum sibiricum compound granule and its preparation method, belonging to the technical field of modern processing of traditional Chinese medicine and development of functional foods. The method includes: screening Lactobacillus casei, Lactobacillus fermentum, and Monascus purpureus as a mixed-culture combination based on the CRITIC-WASPAS multi-index comprehensive evaluation method; inoculating the mixed-culture combination into Polygonatum sibiricum culture medium to obtain Polygonatum sibiricum fermentation broth; compounding the Polygonatum sibiricum fermentation broth with aqueous extracts of sweet tea, Eucommia ulmoides, white kidney bean, and Litsea cubeba, and adding low-chain inulin, pea protein peptides, and gellan gum as co-solvents to obtain a compound solution; and freeze-drying to obtain the mixed-culture fermented Polygonatum sibiricum compound granule. This invention objectively screens dominant strains through a multi-index comprehensive evaluation method, and the compound system and co-solvents synergistically enhance the effect, improving the utilization rate of active ingredients. The resulting granule dissolves rapidly, disperses evenly, and has good antioxidant, hypoglycemic, and lipid-lowering activities.

The utility model discloses an efficient fructus amomi baking machine based on a circulating hot air system, and relates to the technical field of baking equipment, the efficient fructus amomi baking machine comprises a baking box body, a main box door, a baking net disc and a fan box, the inner side of the fan box is provided with an electric heating net and a circulating fan, the lower side of the baking net disc is provided with a plurality of blowpipes at equal intervals, and the upper surface of each blowpipe is densely provided with blowing holes; and a plurality of air return pipes are arranged on the upper side of the baking net disc at equal intervals, air return holes are densely distributed in the air return pipes, and one end of each air return pipe communicates with the side, away from the baking box body, of the fan box through a circulating air pipe. According to the fructus amomi baking device, the electric heating net is matched with the circulating fan, air flow heated by the electric heating net can be uniformly distributed to the lower sides of the baking net discs through the air blowing pipe, fructus amomi can be fully baked through the hot air flow blown from bottom to top, and the hot air flow after heating the fructus amomi is uniformly sucked back through the air return pipe; and the baking efficiency is higher.

The essential oil from Litsea species can be used as a preservative in cleaning compositions for hair, skin or scalp, in laundry detergent compositions or in cleaning compositions for hard surfaces.

The invention relates to a litsea cubeba leaf spice, also relates to a litsea cubeba leaf spice as well as a preparation method and application thereof, and belongs to the technical field of tobacco spices. The litsea cubeba leaf spice is prepared by the following method: (1) inoculating trichoderma harzianum into a liquid culture medium, culturing for 3-5 days at the temperature of 20-35 DEG C, and centrifuging to obtain supernate; (2) spraying the fermentation liquor in the step (1) on the surfaces of the litsea cubeba cotyledons according to the mass ratio of 5-25%, and culturing for 0.5-7 days at the temperature of 15-60 DEG C; (3) drying the fermented litsea cubeba leaves, extracting with a 50-100% ethanol-water solution, and concentrating to a fixed mass ratio; and (4) refrigerating the litsea cubeba leaf extract obtained in the step (3) at-50-10 DEG C for 4-48 hours, and filtering to obtain the litsea cubeba leaf spice. The litsea cubeba leaf spice prepared by the method can be applied to cigarettes, low-grade flue-cured tobacco leaves and cigar tobacco leaves, and the litsea cubeba leaf spice is wide in available raw material application range and suitable for large-scale production.

The application discloses a hydrogel bead containing essential oil and a preparation method and application thereof, and relates to the technical field of fruit preservation. The hydrogel bead comprises a gel matrix and composite essential oil dispersed in the gel matrix; the gel matrix is a complex system comprising bentonite and sodium alginate; and the composite essential oil comprises one or more of Chinese plantain tree essential oil, tea tree essential oil and oregano essential oil. The preparation method comprises the following steps: dispersing bentonite in deionized water, then adding sodium alginate and uniformly mixing to obtain a mixed solution a; uniformly mixing Chinese plantain tree essential oil, tea tree essential oil and oregano essential oil to obtain composite essential oil; dispersing the composite essential oil in the mixed solution a to obtain a mixed solution b; and adding the mixed solution b drop by drop into a calcium chloride solution for crosslinking to obtain the hydrogel bead. The hydrogel bead of the application can effectively inhibit the breeding of harmful microorganisms and the oxidation deterioration of fruits during storage, avoid the safety risks caused by chemical residues, and further significantly prolong the shelf life of fruits, so as to guarantee the postharvest quality of fruits.

This invention relates to the field of pharmaceutical technology, specifically to a combination of traditional Chinese medicine (TCM) preparations for antibacterial and health-promoting treatment throughout the entire life cycle, and its method of use. The kit comprises three independent preparations: a rapid-release protective spray using Litsea cubeba essential oil nanoemulsion as a solvent, loaded with a compound alcoholic extract derived from Polygonum cuspidatum, Senecio scandens, Clematis chinensis, and Glycyrrhiza uralensis in a weight ratio of 4:3:1:1; a sustained-release repair gel patch using a thermosensitive poloxamer F127 and gelatin-sodium alginate double-network hydrogel as a matrix, encapsulating a concentrated aqueous extract obtained from freeze-dried powders of Polygonum cuspidatum, Portulaca oleracea, dried ginger, Bletilla striata, and honey in a weight ratio of 4:2:1:2:1; and a soluble healing-promoting bath film, a biodegradable film made from polyvinyl alcohol and carboxymethyl chitosan via a casting method, in which ultrafine powders of the core active pharmaceutical ingredient and nano-silver-bioglass composite material are dispersed. This application aims to construct a multi-layered, responsive product system from rapid protection to deep repair.

The application provides a microcapsule structure and a preparation method and application thereof, and the microcapsule structure comprises Chinese Litsea cubeba essential oil, beta-cyclodextrin, chitosan and inulin; the Chinese Litsea cubeba essential oil is located in the porous structure of the beta-cyclodextrin to form a complex; the complex is located in a three-dimensional structure formed by graft polymerization of the chitosan and the inulin to form the microcapsule structure. The Chinese Litsea cubeba essential oil is coated in the beta-cyclodextrin-chitosan-inulin to form the microcapsule structure, so that the irritability and volatility of the Chinese Litsea cubeba essential oil are greatly reduced, and the water solubility, stability and antibacterial activity are improved.

The invention relates to a litsea cubeba tissue culture method and a culture medium thereof. The culture medium for litsea cubeba tissue culture comprises a specific induction culture medium, a proliferation culture medium and a rooting culture medium. The method comprises the following steps: taking a semi-lignified new branch which is newly germinated after being trimmed and has an axillary bud as an explant, sequentially performing induced culture, multiplication culture and rooting culture after terminal PPM disinfection, and further domesticating and transplanting to obtain a tissue culture seedling; wherein the induction culture medium is based on WPM and contains sodium citrate and PPM to synergistically control bacteria and brown, the proliferation culture medium is based on WPM and contains sodium citrate and Meta-Topolin to improve proliferation times and inhibit base browning, and the rooting culture medium is based on WPM and contains activated carbon to control brown and promote roots. According to the method, a reproducible and cost-controllable technical path is provided for excellent litsea cubeba germplasm resource preservation and industrialized seedling production.