Essential oil composition and use thereof

Abstract

The present application relates to the technical field of plant essential oil, and particularly discloses an essential oil composition and application thereof.The essential oil composition comprises the following components: carvacrol, thymol, lemon oil, peppermint oil, bay leaf oil, patchouli oil, geranium oil, perilla oil, cinnamon oil, clove oil and boldo oil.The essential oil composition provided by the present application is natural, environment-friendly, has good sterilization effect, comfortable smell, no obvious skin irritation, harmless to human body, and friendly to the environment, and can be applied to the preparation of antimicrobial products.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of plant essential oil, and particularly relates to an essential oil composition and application thereof. BACKGROUND

[0002] With the expansion of people's activities and the diversity of lifestyle, a large amount of household garbage is generated by human beings every day, which contains a large amount of bacteria and viruses, some of which can float in the air with the flow of air, threatening people's health. Many highly pathogenic microbial pathogens cause infectious diseases that break out and spread rapidly. Such infectious diseases can even spread across several provinces, several countries or even the whole world in a short time, that is, rapidly develop into a “pandemic”.

[0003] During the pandemic, disinfection is an important work of epidemic prevention and control. At present, synthetic chemical disinfectants (such as ethanol, peracetic acid, chlorine-containing disinfectants, phenolic disinfectants, etc.), ultraviolet rays, heat, etc. are commonly used to inactivate pathogenic microorganisms worldwide. Long-term use of these disinfectants can cause the increase of bacterial tolerance, making the bactericidal and disinfectant effect gradually weak. Moreover, these disinfectants have a great stimulating effect on the respiratory system, skin and mucous membrane of the human body, and improper use can cause certain harm to the human body.

[0004] Essential oil products based on natural plants have been used to treat infectious diseases and epidemic prevention and control for thousands of years. Plant essential oil, also known as perfume oil or aromatic oil, is a general term for volatile oil liquid with certain fragrance, which is a secondary metabolite produced by plant body, belonging to aliphatic, aromatic, terpenoid compounds and nitrogen and sulfur-containing compounds. Some ingredients in plant essential oil have bactericidal function, but plant essential oil also has the problems of skin irritation and low bactericidal efficiency.

[0005] The killing or inhibition of microorganisms can be divided into multiple levels of sterilization, disinfection, bactericidal, antibacterial and bacteriostatic. Among them, bactericidal refers to the action of killing microorganism vegetative and reproductive bodies (effect evaluation refers to “Disinfection Technical Specification” 2002 edition-2.1.1.7 quantitative killing test and “Disinfection Technical Specification” 2002 edition-2.1.1.9 quantitative killing test); bacteriostatic refers to the action of preventing or inhibiting the growth and reproduction of microorganisms (effect evaluation refers to the bacteriostatic rate detection method of “Disinfection Technical Specification” 2002 edition-2.1.11.3); antibacterial refers to the total of bacteriostatic and bactericidal action.

[0006] Although the patent WO2022 / 068977A2 discloses an antimicrobial essential oil composition composed of carvacrol, thymol, lemon oil, Asian mint oil, etc., which has significant antiviral (such as anti-coronavirus) and antibacterial (such as good antibacterial effect on Escherichia coli, Staphylococcus aureus and Candida albicans) effects, etc., the patent does not disclose the bactericidal effect of the essential oil composition and its diluent, and the air disinfection effect of the essential oil composition is not ideal.

[0007] Therefore, it is necessary to provide a plant essential oil product which is harmless to human body, friendly to environment, in line with the trend of green environmental protection and sustainable development, and has antimicrobial activity, especially good bactericidal effect and air disinfection effect. SUMMARY

[0008] In view of the above technical problems existing in the prior art plant essential oil, the present application provides an essential oil composition and its application.

[0009] To achieve the above-mentioned application purposes, the present application adopts the following technical solutions:

[0010] An essential oil composition, comprising the following components in mass fraction: carvacrol 3-7 parts; thymol 0.08-0.4 parts; lemon oil 0.076-1.45 parts; mint oil 0.08-1.24 parts; bay leaf oil 0.24-0.45 parts; patchouli oil 0.01-0.42 parts; geranium oil 0.004-0.24 parts; perilla oil 0.04-0.4 parts; cinnamon oil 35-60 parts; clove oil 0.08-22 parts and litsea cubeba oil 0.08-17 parts.

[0011] Further, the essential oil composition comprises the following components in mass fraction: carvacrol 4.5-6.8 parts; thymol 0.2-0.4 parts; lemon oil 0.95-1.45 parts; mint oil 0.7-1.24 parts; bay leaf oil 0.3-0.45 parts; patchouli oil 0.01-0.02 parts; geranium oil 0.12-0.24 parts; perilla oil 0.25-0.4 parts; cinnamon oil 50-55 parts; clove oil 0.08-3.2 parts and litsea cubeba oil 0.08-1 parts.

[0012] Further, the essential oil composition comprises the following components in mass fraction: carvacrol 3-5 parts; thymol 0.08-0.12 parts; lemon oil 0.08-0.12 parts; mint oil 0.08-0.12 parts; bay leaf oil 0.24-0.36 parts; patchouli oil 0.2-0.4 parts; geranium oil 0.004-0.008 parts; perilla oil 0.04-0.08 parts; cinnamon oil 35-45 parts; clove oil 16-22 parts and litsea cubeba oil 12-17 parts.

[0013] Further, the essential oil composition comprises the following components in mass fraction: carvacol 5.673 parts; thymol 0.276 parts; lemon oil 1.208 parts; mint oil 1.035 parts; bay leaf oil 0.345 parts; patchouli oil 0.017 parts; geranium oil 0.173 parts; perilla oil 0.328 parts; cinnamon oil 55 parts; clove oil 0.1 part and litsea cubeba oil 0.1 part.

[0014] Further, the essential oil composition comprises the following components in mass fraction: carvacol 4.5 parts; thymol 0.39 parts; lemon oil 0.95 parts; mint oil 0.75 parts; bay leaf oil 0.45 parts; patchouli oil 0.02 parts; geranium oil 0.24 parts; perilla oil 0.4 parts; cinnamon oil 52 parts; clove oil 3 parts and litsea cubeba oil 0.5 parts.

[0015] Further, the essential oil composition comprises the following components in mass fraction: carvacol 3.9 parts; thymol 0.1 parts; lemon oil 0.095 parts; mint oil 0.1 parts; bay leaf oil 0.3 parts; patchouli oil 0.35 parts; geranium oil 0.005 parts; perilla oil 0.05 parts; cinnamon oil 40 parts; clove oil 20 parts and litsea cubeba oil 15 parts.

[0016] Further, the essential oil composition comprises the following components in mass fraction: carvacol 3 parts; thymol 0.12 parts; lemon oil 0.1 parts; mint oil 0.12 parts; bay leaf oil 0.25 parts; patchouli oil 0.2 parts; geranium oil 0.008 parts; perilla oil 0.07 parts; cinnamon oil 45 parts; clove oil 17 parts and litsea cubeba oil 13 parts.

[0017] Further, the essential oil composition further comprises the following components in mass fraction: osmanthus oil 5-12 parts and lemon grass oil 2-12 parts.

[0018] The mint oil is one or both of Asian mint oil and peppermint essential oil (European mint oil).

[0019] The application further provides a preparation method of the essential oil composition, comprising the following steps: mixing the components at normal temperature and pressure, stirring until the thymol is dissolved, and obtaining the essential oil composition.

[0020] Compared with the prior art, the essential oil composition provided by the application has complementary and additive activities among various essential oil components, unexpected synergies are achieved compared with single essential oil, and significantly better antimicrobial (the "antimicrobial" refers to the activity of the product used to reduce / kill microorganisms) properties are obtained, the essential oil composition has good bactericidal effects on Escherichia coli, Staphylococcus aureus and Candida albicans, and has good killing effects on Staphylococcus aureus in the air. Meanwhile, the essential oil composition is made of pure natural plant essential oil, and compared with traditional synthetic chemical disinfecting and sterilizing products, the essential oil composition is natural, environmentally friendly, has good sterilizing and disinfecting effects, comfortable smell, no obvious skin irritation, and is friendly to the environment.

[0021] The application also provides application of the essential oil composition in antimicrobial.

[0022] Further, the microorganism is selected from bacteria, fungi and combinations thereof.

[0023] Further, the bacteria are one or more of gram-negative bacteria or gram-positive bacteria.

[0024] Further, the gram-negative bacteria include one or more of Catarrhalis, Escherichia coli, Pseudomonas aeruginosa and Shigella; the gram-positive bacteria include one or more of Streptococcus pneumoniae, Staphylococcus albus, Staphylococcus aureus, Propionibacterium acnes and Staphylococcus epidermidis; and the fungi include one or more of mold, yeast, Candida albicans and Malassezia.

[0025] The application also provides an article comprising the essential oil composition.

[0026] The article can include an article capable of disinfecting and sterilizing microorganisms in the environment, on a surface or on the human body, that is, an article having no toxicity, no irritation and no corrosion to the human body, being friendly to the environment and capable of reducing or killing harmful microorganisms in the environment, on a surface or on the human body.

[0027] Further, the article includes an article for inhibiting or killing microorganisms on one or more of a hard surface, a porous surface, a textile surface, skin, mucous membrane and hair or an article for inhibiting or killing microorganisms in the air.

[0028] Further, the article is one or more selected from a pharmaceutical product, a disinfecting product, a protective product, a daily chemical product and a textile product.

[0029] Further, the article is a cosmetic product, a personal cleaning and care product or a daily washing product.

[0030] Further, the product is one or more selected from the group consisting of a bouncy ball, a wet wipe, a fabric disinfectant, a soap, a toilet soap, a laundry detergent, a dishwashing detergent, an acne cream, a hand cream, an air freshener, a fragrance, a toothpaste, a mouth spray, a nose spray, a hand sanitizer, a shampoo, a hair conditioner, a body wash, an air disinfectant gel, an air disinfectant, an oil cleaner, a skin disinfectant, and a skin disinfectant spray.

[0031] Further, the product is one or more selected from the group consisting of an underwear, an underpants, a sanitary napkin, a bed sheet, a sock, and an insole.

[0032] Further, the product is one or more selected from the group consisting of a civilian mask, a medical mask, a protective glove, and a protective clothing.

[0033] Further, the product is a bouncy ball mask.

[0034] The present application also provides a preparation method of the product, comprising the following steps:

[0035] S1, preparing the essential oil composition into essential oil masterbatch or essential oil microcapsule or essential oil bouncy ball;

[0036] S2, combining the essential oil masterbatch or essential oil microcapsule or essential oil bouncy ball with other components of the product to obtain the product.

[0037] Further, when the product is a civilian mask or a medical mask, the step S2 comprises adding the essential oil masterbatch or essential oil microcapsule or essential oil bouncy ball into the civilian mask or the medical mask.

[0038] The present application also provides a disinfectant comprising the essential oil composition.

[0039] Further, the disinfectant comprises the following mass fractions of raw material components: the essential oil composition 5-10%; a solvent 65-70%; a solubilizing agent 23-28%; and an antioxidant 0.1-0.2%.

[0040] Further, the solvent is deionized water; the solubilizing agent is a mixture of polysorbate-20 and PPG-26 butyl alcohol polyether-26 / PEG-40 hydrogenated castor oil with a mass ratio of 2-4:1; and the antioxidant is disodium ethylenediaminetetraacetate.

[0041] Further, the preparation method of the disinfectant comprises the following steps:

[0042] (1) completely dissolving the antioxidant in the solvent to obtain a premix A;

[0043] (2) mixing the solubilizing agent with the essential oil composition to obtain a premix B;

[0044] (3) Pour the premix B into the premix A and stir until uniform, adjust pH value to 4.0-8.0 with citric acid or sodium citrate, and obtain the disinfectant.

[0045] The disinfectant is used for inhibiting or killing microorganisms on one or more of a hard surface, a porous surface, a textile surface, skin, mucous membrane, and hair.

[0046] The disinfectant is prepared by diluting the essential oil composition, has a comfortable smell, has a good killing effect (bactericidal rate > 99.999%) on Escherichia coli, Staphylococcus aureus, and Candida albicans, has a light irritation in a skin irritation test, can be used for skin contact, and is used for sterilization and disinfection of daily objects and human bodies.

[0047] The application further provides an air disinfectant comprising the essential oil composition.

[0048] The air disinfectant is composed of pure plant essential oils, has a moderate and non-irritating smell, has a good air sterilization and disinfection effect, has a staphylococcus aureus killing rate (average value) of ≥99.91%, and can be used in air disinfection and freshening products. DETAILED DESCRIPTION

[0049] In order to make the purpose, technical scheme and advantages of the application clearer, the application is further described in detail below with reference to examples. It should be understood that the specific examples described herein are only used to explain the application and do not limit the application.

[0050] The carvacrol (CARVACROL) and thymol (THYMOL) in the application are volatile oils extracted from plants such as thyme, oregano leaves and sweet oregano leaves and purified.

[0051] The lemon oil in the application is a volatile oil extracted from the peel of lemon (CITRUS LIMON) plants.

[0052] The mint oil (MENTHA HAPLOCALYX OIL) in the application is a volatile oil obtained by water vapor distillation, freezing and partial brain removal of the stems and leaves of plants in the Lamiaceae family.

[0053] The laurel leaf oil (LAURUS NOBILIS LEAF OIL) in the application is a volatile oil extracted from laurel leaves (or fruits) of the Lauraceae family.

[0054] The pogostemon cablin oil (POGOSTEMON CABLIN OIL) in the application is a volatile oil extracted from the leaves and stems of plants in the Lamiaceae family.

[0055] The pelargoneum oil according to the present application refers to the volatile oil extracted from the flowers, stems or leaves of Pelargonium graveolens.

[0056] The perilla oil according to the present application refers to the volatile oil extracted from the leaves of Perilla ocymoides.

[0057] The syzygium aromaticum oil according to the present application refers to the volatile oil extracted from the flowers, stems or leaves of Syzygium aromaticum.

[0058] The cinnamomum cassia oil according to the present application refers to the volatile oil obtained by distillation of the barks, branches or leaves of Cinnamomum cassia.

[0059] The litsea cubeba oil according to the present application refers to the volatile oil extracted from the fruits of Litsea cubeba.

[0060] Examples 1-7

[0061] According to the formulations in Table 1 below, the components are mixed at normal temperature and pressure, and stirred until the thymol is dissolved, to prepare the essential oil compositions of Examples 1-7.

[0062] Table 1 Formulations of essential oil compositions of examples

[0063]

[0064]

[0065] [Note]: The numbers in the above table represent the mass parts.

[0066] Comparative Examples 1-8

[0067] In order to further illustrate the properties of the essential oil compositions provided by the present application, the essential oil compositions of Comparative Examples 1-8 are prepared according to the component formulations in Table 2 below, wherein Comparative Examples 1, 3, 4, 5, 7 and 8 are prepared by the same method as the examples, and Comparative Examples 2 and 6 are prepared by mixing the components at normal temperature and pressure.

[0068] Table 2 Formulations of essential oil compositions of comparative examples

[0069]

[0070] [Note]: The numbers in the above table represent the mass parts; "-" indicates that the corresponding component is not included.

[0071] The essential oil compositions obtained in Examples 1-3 and the essential oil compositions obtained in Comparative Examples 1-4 were mixed with the raw materials in the mass fractions shown in Table 3, and poly sorbate-20, PPG-26 butyl alcohol polyether-26 / PEG-40 hydrogenated castor oil, disodium ethylenediaminetetraacetate, and deionized water were prepared into essential oil diluent (production batch numbers 2022110301; 2022110302; 2022110303; 2022110304; 2022110305; 2022110306; 2022110307) according to the above-mentioned preparation method of disinfectant, i.e. disinfectant.

[0072] Table 3 Disinfectant component ratio

[0073]

[0074]

[0075] In order to verify the bactericidal properties of the essential oil composition provided by the present application, the above-mentioned disinfectants containing the essential oil compositions of Examples 1-3 and Comparative Examples 1-4 were tested as follows.

[0076] Test Example 1

[0077] According to the Quantitative Killing Test of Disinfection Technical Specification 2002 Edition-2.1.1.7 and the Quantitative Killing Test of Disinfection Technical Specification 2002 Edition-2.1.1.9, the above-mentioned disinfectants were tested for bactericidal performance.

[0078] 1. Escherichia coli (8099) and Staphylococcus aureus (ATCC 6538) bactericidal test

[0079] Test bacteria and bacterial solution preparation

[0080] Sterile hard water was used for preparation, and the concentration was 1.25 times the concentration to be tested, and was placed in a 20℃±1℃ water bath for standby.

[0081] (1) Prepare the experimental bacterial suspension with a concentration of 1×10 8 cfu / ml to 5×10 8 cfu / ml.

[0082] (2) Sterile large test tubes for disinfection test were prepared, 0.5ml of the experimental bacterial suspension was added first, then 0.5ml of organic interfering substances was added, mixed well, placed in a 20℃±1℃ water bath for 5min, then 4.0ml of the above-mentioned concentration of disinfectant was taken with a sterile pipette and injected into it, mixed quickly and immediately recorded the time.

[0083] (3) After the test bacteria and the disinfectant interacted for a predetermined time, 0.5ml of the test bacteria and disinfectant mixture was taken and added to 4.5ml of sterilized neutralizing agent, mixed well.

[0084] (4) After the neutralizer is added to each tube of the test bacteria and disinfectant mixture for 10 minutes, 1.0 ml of the sample solution is taken from each tube and the number of viable bacteria is determined by the viable bacteria culture counting method. Two plates are inoculated with each tube of sample solution. If the number of colonies growing on the plate is large, a series of 10-fold dilutions can be performed and then the viable bacteria culture counting can be performed.

[0085] (5) At the same time, use the diluent instead of the disinfectant and conduct a parallel test as a positive control.

[0086] (6) All test samples were cultured in a 37°C incubator. The bacterial propagules were cultured for 48 hours and the final results were observed. The bacterial spores were cultured for 72 hours and the final results were observed.

[0087] The test was repeated three times, and the viable bacterial concentration (cfu / ml) of each group was calculated and converted into a logarithmic value (N). The killing logarithmic value was then calculated as follows:

[0088] Killing logarithm (KL) = logarithm of the average viable bacteria concentration in the control group (No) - logarithm of the viable bacteria concentration in the test group (Nx)

[0089] When calculating the logarithmic kill value, use two decimal places and round off the numbers. If the average colony count of the disinfection test group after disinfection is less than or equal to 1, its logarithmic kill value is greater than or equal to the logarithmic value of the average viable bacterial concentration of the control group before the test.

[0090] Calculation of sterilization rate: The test was repeated three times, and the viable bacterial concentration (cfu / ml) of each group was calculated and converted into a logarithmic value (N). The killing logarithm was then calculated according to the following formula.

[0091]

[0092] Where: X is the sterilization rate, %; N C is the average colony count of the control sample, cfu / plate; N S is the average colony count of the tested samples, cfu / plate.

[0093] 2. Candida albicans (ATCC 10231) bactericidal test

[0094] (1) Phosphate buffer (PBS, 0.03 mol / L, pH 7.2).

[0095] (2) Hard water for diluting disinfectants: see Appendix A.

[0096] (3) Organic interfering substances: see Appendix A.

[0097] (4) Graduated pipette (0.1ml, 1.0ml, 5.0ml).

[0098] (5) Constant temperature water bath.

[0099] (6) Spray device (for spray disinfection test).

[0100] (7) Electric mixer.

[0101] (8) Timing device.

[0102] Preparation of fungal suspension

[0103] (1) Preparation of Candida albicans suspension

[0104] 1) Take a freeze-dried bacterial culture tube, open it aseptically, use a capillary pipette to add an appropriate amount of Sandcastle liquid culture base into the tube, and gently pipette several times to melt and disperse the bacteria. Take a test tube containing 5.0ml~10.0ml Sandcastle liquid culture medium, drop a small amount of bacterial suspension into it, and incubate it at 37℃ for 18h~24h. Use an inoculation loop to take the bacterial suspension of the first generation culture, streak it on the Sandcastle agar medium plate, and incubate it at 37℃ for 18h~24h. Pick out the typical colonies from the above second generation culture, inoculate them on the Sandcastle agar slant, and incubate them at 37℃ for 18h~24h to obtain the third generation culture. Seal it and store it at 4℃ for no more than 6 weeks.

[0105] 2) For the test, take a third-generation slant culture and serially subculture it on a Sandcastle agar slant using the same method as for the third generation. Take a fresh fifth- or sixth-generation Sandcastle agar slant culture (18 to 24 hours old) and pipette 3 to 5 ml of diluent into the slant test tube using a 5.0 ml pipette. Repeatedly pipette and aspirate to remove the bacterial lawn. Then, pipette the wash solution into another sterile test tube using a 5.0 ml pipette. Mix with an electric mixer for 20 seconds or tap the tube 80 times in the palm of your hand to ensure a uniform suspension of the Candida albicans.

[0106] 3) In the suspension sterilization test, the bacterial content of the experimental bacterial suspension is 1×10 7 cfu / ml~5×10 7 cfu / ml; prepare bacterial plates.

[0107] 4) Store the bacterial suspension in a refrigerator at 4°C until use.

[0108] Trial Groups

[0109] The experiment was divided into the following groups:

[0110] (1) Experimental group: the disinfectant concentration (essential oil dilution stock solution) and the action time were 2 min, and the killing ability of Candida albicans was determined.

[0111] (2) Positive control group, using hard water instead of the disinfectant solution, and performing the test according to the prescribed procedure. The results obtained represent the initial concentration of the test bacteria contained in the test system, and are used to calculate the killing logarithm of the test bacteria by the disinfecting factor.

[0112] (3) Negative control group, observing whether the related solutions and media used in the same test are contaminated.

[0113] (4) Suspension quantitative germicidal test, observing the final results in a 37°C incubator for 48 h.

[0114] The test is repeated three times, the viable bacterial concentration (cfu / ml) of each group is calculated, and converted into logarithm (N), and then the killing logarithm is calculated according to the following formula:

[0115] Killing logarithm (KL) = logarithm of the average viable bacterial concentration of the control group (No) - logarithm of the viable bacterial concentration of the test group (Nx)

[0116] When calculating the killing logarithm, the last two digits after the decimal point are taken, and numerical rounding can be performed. If the average colony count of the test bacteria after disinfection treatment is less than or equal to 1, the killing logarithm is greater than or equal to the logarithm of the average viable bacterial concentration of the control group before the test.

[0117] Germicidal rate calculation: the test is repeated three times, the viable bacterial concentration (cfu / ml) of each group is calculated, and converted into logarithm (N), and then the killing logarithm is calculated according to the following formula.

[0118]

[0119] In the formula, X is the germicidal rate, %; N C is the average colony count of the control sample, cfu / piece; N S is the average colony count of the test sample, cfu / piece.

[0120] The action concentration, action time, and test conditions are the same, and the germicidal efficacy of different disinfectants on a certain bacteria is compared. In the formula, Y is the germicidal efficacy multiple; N is the average colony count of the control sample, A is the average colony count of the disinfectant, cfu / piece; and B is the average colony count of the disinfectant, cfu / piece.

[0121] or

[0122] The germicidal test results of the disinfectants of different batches corresponding to Examples 1-3 and Comparative Examples 1-4 are shown in Table 4.

[0123] Table 4

[0124]

[0125] From the data in the table, the essential oil composition provided by the application has good killing effect on escherichia coli, staphylococcus aureus and candida albicans.

[0126] The values embodied in the table, the sterilization rate is 99.999%, 99.99%, 99.9% seemingly small difference in value, reflected in the sterilization raw material sterilization ability but 10 times, 100 times difference. In the laboratory according to the method of "disinfection technical specification" 2002 edition of the germicidal efficacy identification experiment, when the killing logarithm value is 3, the corresponding sterilization rate is 99.9%; the killing logarithm value is 4, the corresponding sterilization rate is 99.99%; the killing logarithm value is 5, the corresponding sterilization rate is 99.999%. Generally, the relative mildness of herbal antibacterial / antibacterial raw materials is difficult to achieve ≥3 (sterilization rate ≥99.9%) in killing logarithm value of microorganisms, therefore, the present work is very important and rare to find that the killing logarithm value of multiple microorganisms is ≥4 (sterilization rate ≥99.99%) or even ≥5 (sterilization rate ≥99.999%) after screening multiple essential oil compounds and synergistic effect.

[0127] Test example 2

[0128] According to "disinfection technical specification" 2002 edition, part 2.3.3, the above disinfectant containing essential oil composition of examples 1-3 and comparative examples 1-4 was subjected to a complete skin irritation test.

[0129] 24h before the test, the hair on both sides of the rabbit's back spine was shaved. The range of depilation was about 3cm*3cm on the left and right sides. 0.5mL of the test substance was applied to one side of the skin, then covered with a layer of non-irritating plastic film, and fixed with non-irritating adhesive tape. The other side of the skin was used as a blank control. The closed test was used, and the application time was 4h. After the experiment, the residual test substance was removed with warm water.

[0130] The skin reaction results of the test site were observed at 1h, 24h and 48h after the test substance was removed, and the skin reaction score was scored according to the skin irritation reaction score table. According to the highest integral mean value of each observation point at 1h, 24h and 48h, the skin irritation intensity was determined according to the skin irritation intensity grading standard table. The skin irritation test results of the disinfectants corresponding to examples 1-3 and comparative examples 1-4 are shown in table 5.

[0131] Table 5 skin irritation test results

[0132]

[0133] From the above data, it can be seen that the essential oil composition provided by the application has light irritation or no irritation to the New Zealand rabbit in the complete skin irritation test under the test conditions.

[0134] In summary, through the comparison of the test results of Examples 1-3 and Comparative Examples 1-4, it can be seen that the essential oil composition of the present application is obtained by compounding and ratio control of various essential oil components. The essential oil composition of the present application has synergistic effect by compounding carvacrol, thymol, lemon oil, peppermint oil, bay leaf oil, patchouli oil, pelargonium oil, perilla oil with cinnamon oil, clove oil, and beilschmiedia oil, which improves the bactericidal efficacy and skin irritation of the essential oil composition. At the same time, by adding osmanthus oil and lemon grass oil as the flavoring component, the irritation of the essential oil composition is further optimized. The essential oil composition provided by the present application can be applied to the preparation of antimicrobial products, such as disinfectants.

[0135] At the same time, in order to verify the air disinfection effect of the essential oil composition of the present application, the essential oil composition prepared according to the formula and preparation method of Examples 4-6 and Comparative Examples 5-8 of the present application was used as an air disinfectant (production batch numbers 2022110401, 2022110402, 2022110403, 2022110404, 2022110405, 2022110406 and 2022110407). The air disinfectant was tested as follows.

[0136] Test Example 3

[0137] According to the Disinfection Technical Specification 2002 Edition 2.1.3, the above-mentioned air disinfectant was subjected to air disinfection effect laboratory test.

[0138] 1. Equipment

[0139] Test sample: air disinfectant, production batch numbers 2022110401, 2022110402, 2022110403, 2022110404, 2022110405, 2022110406 and 2022110407.

[0140] Test strain: Staphylococcus albus 8032 strain with 4th generation, strain source: Guangdong Microbial Culture Collection Center.

[0141] Neutralizing agent ingredients and concentration: 3% Tween-80, 0.5% sodium thiosulfate, 0.5% L-histidine, 0.5% peptone, 0.85% sodium chloride, 1.43% lecithin, 0.1% cysteine in PBS solution.

[0142] Culture medium name and batch number: nutrient agar medium containing neutralizing agent (batch number: 20220606).

[0143] Volume of aerosol chamber: 1 m 3 .

[0144] Instruments used: biochemical incubator (No.: OFM-B-S051), water bath (No.: OFM-B-K038), liquid impact microbial aerosol sampler (No.: OFM-B-S041), and biological safety cabinet (No.: OFM-B-S028).

[0145] 2. Methods

[0146] Testing environment: temperature 23.8°C, relative humidity 62%.

[0147] Method description: In 1m 3 In the test room, the fan was used to stir while spraying. After the spraying was completed, the fan was continued to stir for 5 minutes, and then it was allowed to stand for 5 minutes. The number of positive control bacteria in the air of the aerosol room reached 5.0×10 4 cfu / m 3 ~5.0x10 6 cfu / m 3 . A liquid impact microbial aerosol sampler was used to sample the aerosol chambers of the experimental group and the control group before disinfection. Sampling was performed at a flow rate of 11 L / min, the volume of the sampling liquid was 20 mL, and the sampling time for both the experimental group and the control group was 2 minutes. The sample liquid was added to the nebulizer, and the power was turned on to atomize 10 mL of the sample liquid into the air of the aerosol chamber. The door of the room was closed. After 1 hour of disinfection, the aerosol chamber after disinfection was sampled with a sampling liquid containing a neutralizer at the same flow rate. The sampling time for both the experimental group and the control group was 2 minutes. The collected plates were placed in a 37°C incubator for 48 hours and the results were observed. The experiment was repeated 3 times.

[0148] 3. Results

[0149] Under the conditions of ambient temperature of 23.8℃ and relative humidity of 62%, add the sample solution into the atomizer provided by the client, turn on the power and atomize 10mL of sample solution into 1m 3 The aerosol was allowed to act in the chamber for 1 hour. The sterilization rates of each batch of air disinfectant against Staphylococcus albus (all three test results were no less than the data in the table) are shown in Table 6.

[0150] Table 6

[0151]

[0152] From the above results, it can be seen that the essential oil composition prepared in the embodiment of the present invention is used as an air disinfectant and added to the atomizer. The power is turned on and 10 mL of the sample liquid is atomized into 1 m 3 The aerosol was applied in the chamber for 1 hour and the test was repeated 3 times. The sterilization rate of Staphylococcus aureus in the air was detected to be between 99.91% and 99.98%, indicating that the essential oil composition provided by the embodiment of the present invention has a good sterilization effect on Staphylococcus aureus in the air.

[0153] In conclusion, through the comparison of the test results of Examples 4-6 and Comparative Examples 5-8, it can be seen that the essential oil composition obtained by compounding and ratio adjustment of various essential oil components has good killing effect on Staphylococcus albus in the air. The essential oil composition provided by the present application can be applied to the preparation of microbial inhibition or killing products in the air.

[0154] The above merely describes the preferred embodiments of the present application and is not used to limit the present application, and any modification, equivalent replacement or improvement made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. An essential oil composition, characterized in that The invention is composed of the following components in parts by mass: 3-7 parts of carvacrol; 0.08-0.4 parts of thymol; 0.076-1.45 parts of lemon oil; 0.08-1.24 parts of peppermint oil; 0.24-0.45 parts of bay leaf oil; 0.01-0.42 parts of patchouli oil; 0.004-0.24 parts of geranium oil; 0.04-0.4 parts of perilla oil; 35-60 parts of cinnamon oil; 0.08-22 parts of clove oil and 0.08-17 parts of litsea cubeba oil.

2. The essential oil composition according to claim 1, wherein The invention is composed of the following components in parts by mass: 4.5-6.8 parts of carvacrol; 0.2-0.4 parts of thymol; 0.95-1.45 parts of lemon oil; 0.7-1.24 parts of peppermint oil; 0.3-0.45 parts of bay leaf oil; 0.01-0.02 parts of patchouli oil; 0.12-0.24 parts of geranium oil; 0.25-0.4 parts of perilla oil; 50-55 parts of cinnamon oil; 0.08-3.2 parts of clove oil and 0.08-1 part of litsea cubeba oil.

3. The essential oil composition according to claim 1, wherein The invention is composed of the following components in parts by mass: 3-5 parts of carvacrol; 0.08-0.12 parts of thymol; 0.08-0.12 parts of lemon oil; 0.08-0.12 parts of peppermint oil; 0.24-0.36 parts of bay leaf oil; 0.2-0.4 parts of patchouli oil; 0.004-0.008 parts of geranium oil; 0.04-0.08 parts of perilla oil; 35-45 parts of cinnamon oil; 16-22 parts of clove oil and 12-17 parts of litsea cubeba oil.

4. The essential oil composition according to claim 2, wherein The invention is composed of the following components in parts by mass: 5.673 parts of carvacrol; 0.276 parts of thymol; 1.208 parts of lemon oil; 1.035 parts of peppermint oil; 0.345 parts of bay leaf oil; 0.017 parts of patchouli oil; 0.173 parts of geranium oil; 0.328 parts of perilla oil; 55 parts of cinnamon oil; 0.1 parts of clove oil and 0.1 parts of litsea cubeba oil.

5. The essential oil composition according to claim 2, wherein The invention is composed of the following components in parts by mass: 4.5 parts of carvacrol; 0.39 parts of thymol; 0.95 parts of lemon oil; 0.75 parts of peppermint oil; 0.45 parts of bay leaf oil; 0.02 parts of patchouli oil; 0.24 parts of geranium oil; 0.4 parts of perilla oil; 52 parts of cinnamon oil; 3 parts of clove oil and 0.5 parts of litsea cubeba oil.

6. The essential oil composition according to claim 3, wherein The invention is composed of the following components in parts by mass: 3.9 parts of carvacrol; 0.1 part of thymol; 0.095 part of lemon oil; 0.1 part of peppermint oil; 0.3 part of bay leaf oil; 0.35 part of patchouli oil; 0.005 part of geranium oil; 0.05 part of perilla oil; 40 parts of cinnamon oil; 20 parts of clove oil and 15 parts of litsea cubeba oil.

7. The essential oil composition according to claim 3, wherein The invention is composed of the following components in parts by mass: 3 parts of carvacrol; 0.12 parts of thymol; 0.1 parts of lemon oil; 0.12 parts of peppermint oil; 0.25 parts of bay leaf oil; 0.2 parts of patchouli oil; 0.008 parts of geranium oil; 0.07 parts of perilla oil; 45 parts of cinnamon oil; 17 parts of clove oil and 13 parts of litsea cubeba oil.

8. An essential oil composition, characterized in that The invention is composed of the following components in parts by mass: 3-7 parts of carvacrol; 0.08-0.4 parts of thymol; 0.076-1.45 parts of lemon oil; 0.08-1.24 parts of peppermint oil; 0.24-0.45 parts of bay leaf oil; 0.01-0.42 parts of patchouli oil; 0.004-0.24 parts of geranium oil; 0.04-0.4 parts of perilla oil; 35-60 parts of cinnamon oil; 0.08-22 parts of clove oil; 0.08-17 parts of litsea cubeba oil; 5-12 parts of osmanthus oil and 2-12 parts of lemongrass oil.

9. The method for preparing the essential oil composition according to any one of claims 1 to 7, characterized in that: The steps include: The components are mixed at room temperature and pressure, and stirred until thymol is dissolved.

10. Use of the essential oil composition according to any one of claims 1 to 7 in the preparation of antimicrobial products.

11. The use according to claim 10, characterized in that The microorganism is selected from bacteria, fungi and combinations thereof.

12. The use according to claim 11, characterized in that The bacteria are one or more of Gram-negative bacteria or Gram-positive bacteria.

13. The use according to claim 12, characterized in that The Gram-negative bacteria include one or more of catarrhal cocci, Escherichia coli, Pseudomonas aeruginosa, and Shigella dysenteriae; the Gram-positive bacteria include one or more of Streptococcus pneumoniae, Staphylococcus aureus, Propionibacterium acnes, and Staphylococcus epidermidis; and the fungi include one or more of molds, yeasts, Candida albicans, and Malassezia.

14. A product comprising the essential oil composition according to any one of claims 1 to 7.

15. The article of claim 14, wherein The articles include articles for inhibiting or killing microorganisms on one or more of hard surfaces, porous surfaces, textile surfaces, skin, mucous membranes, hair, or articles for inhibiting or killing microorganisms in the air.

16. The article of claim 15, wherein The product is one or more selected from medicines, disinfection products, protective products, daily chemical products, and textiles.

17. The article of claim 16, wherein The product is a cosmetic, a personal cleaning and care product or a daily washing product.

18. The article of claim 16, wherein The product is one or more selected from the group consisting of popping beads, wet wipes, fabric sterilizing liquid, soap, perfumed soap, laundry detergent, dishwashing liquid, acne cream, hand cream, air freshener, aromatherapy, perfume, toothpaste, oral spray, nasal suction stick, hand soap, shampoo, conditioner, shower gel, air disinfectant, oil stain cleaner, skin disinfectant, and skin antibacterial spray.

19. The article of claim 18, wherein The air disinfectant is air disinfecting gel.

20. The article of claim 16, wherein The product is one or more selected from underwear, underpants, sanitary napkins, bed sheets, socks, and insoles.

21. The article of claim 16, wherein The product is one or more selected from civilian masks, medical masks, protective gloves, and protective clothing.

22. The article of claim 21, wherein The product is a bead-bursting mask.

23. The method for preparing the product according to any one of claims 14 to 22, characterized in that: The steps include: S1, preparing the essential oil composition into essential oil masterbatch, essential oil microcapsule or essential oil bursting beads; S2. Combining the essential oil masterbatch or essential oil microcapsule or essential oil explosive beads with other components of the product to prepare the product according to a conventional method.

24. The preparation method according to claim 23, wherein The product is a civilian mask or a medical mask, and step S2 includes adding the essential oil masterbatch or essential oil microcapsule or essential oil bursting beads to the civilian mask or the medical mask.

25. A disinfectant, characterized in that The invention relates to an essential oil composition comprising the essential oil composition according to any one of claims 1, 2, 4 or 5.

26. The disinfectant according to claim 25, wherein The invention comprises the following raw material components in mass fractions: 5% to 10% of the essential oil composition; 65% to 70% of a solvent; 23% to 28% of a solubilizer; and 0.1% to 0.2% of an antioxidant.

27. The disinfectant according to claim 26, wherein The solvent is deionized water; the solubilizer is a mixture of polysorbate 20 and PPG-26 butane ether-26 / PEG-40 hydrogenated castor oil in a mass ratio of 2 to 4:1; and the antioxidant is disodium edetate.

28. The disinfectant according to claim 27, wherein The preparation method of the disinfectant comprises the following steps: (1) Completely dissolving the antioxidant in the solvent to obtain premix A; (2) mixing the solubilizer and the essential oil composition to obtain a premix B; (3) Add premix B into premix A and stir evenly. Adjust the pH value to 3.0-10.0 with citric acid or sodium citrate to obtain a disinfectant.

29. An air disinfectant, characterized in that The invention relates to an essential oil composition comprising the essential oil composition according to any one of claims 1, 3, 6 or 7.

Images