The invention discloses a
tobacco leaf fermentation and quality improvement method based on
oxygen content regulation and application thereof, and the method comprises the following steps: preparing a tobacco culture medium, crushing tobacco leaves, sieving to obtain tobacco
powder, mixing the tobacco
powder with water in proportion, extracting, filtering and sterilizing to obtain the
tobacco leaf fermentation and quality improvement method; respectively culturing
Klebsiella HNYJ-1 single colonies and
enterobacter HNYJ-4 single colonies to obtain seed solutions, and mixing the seed solutions in proportion to obtain a composite seed solution; and finally, after the tobacco leaves are moistened in a redrying stage and before redrying, spraying the composite seed solution, firstly carrying out aerobic
fermentation in a closed
temporary storage cabinet for 12 hours, then adjusting the
oxygen to 5-20%, carrying out low-
oxygen fermentation for 24 hours, and after fermentation, redrying at high temperature to inactivate the strain. Compared with unfermented and single aerobic / hypoxic fermentation, the method has the advantages that the content of
linalool, 2, 3, 5, 6-
tetramethylpyrazine and other key
aroma components can be greatly increased, the
aroma quality, the
aroma amount and the
fineness of the tobacco leaves are remarkably improved, offensive
odor and
irritation are reduced, the process is adaptive to an existing
processing flow, and extra complex equipment is not needed.