Osmanthus essential oil and application thereof in preparation of pure natural children shampoo capable of removing dandruff, relieving itching and controlling oil

Abstract

The invention belongs to the technical field of plant essential oil, and particularly relates to osmanthus essential oil and application thereof in preparation of pure natural children shampoo capable of removing dandruff, relieving itching and controlling oil. By optimizing the extraction process of the sweet osmanthus essential oil and adopting a method of combining enzymolysis pretreatment with reflux extraction, the extraction efficiency and the active ingredient content of the sweet osmanthus essential oil are remarkably improved. Compared with a traditional extraction method, the extraction process disclosed by the invention has the advantages that natural active ingredients, such as linalool, beta-glucoside and the like, in the osmanthus fragrans essential oil can be better reserved, so that the antibacterial and oil control effects are improved. The osmanthus essential oil is applied to the shampoo for children, so that mild and efficient scalp care can be provided for children. Compared with the traditional shampoo, the shampoo for children not only can clean hair, but also can remarkably reduce the problems of scalp itching, dandruff, excessive grease secretion and the like, and meanwhile, the irritation of chemical components to the scalp of children is avoided.

Description

Technical Field

[0001] The present invention belongs to the technical field of plant essential oils, and particularly relates to an osmanthus essential oil and its application in the preparation of a natural children's shampoo for removing dandruff, relieving itching and controlling oiliness. Background Art

[0002] Children's scalps are relatively sensitive and easily irritated. Therefore, the market demand for mild, safe and specifically effective children's shampoos is increasing day by day. Traditional children's shampoos mainly focus on mild cleaning, but they have limited effects in solving scalp problems (such as dandruff, itching, excessive sebum secretion, etc.). In recent years, consumers' attention to natural ingredients has been continuously increasing, especially showing strong interest in products with natural plant extracts and essential oils. Although there are some children's shampoos added with natural plant extracts on the market, the extraction processes and application effects of these extracts are often not ideal enough to fully exert their natural effects.

[0003] Osmanthus essential oil has a unique aroma and significant care effects, can effectively relieve scalp itching, reduce dandruff, and regulate scalp sebum secretion, and has broad application prospects in the preparation of children's shampoos. However, traditional extraction methods (such as distillation) often cannot efficiently extract the active ingredients in plant essential oils, and partial loss or denaturation of some ingredients may occur during the extraction process. Summary of the Invention

[0004] The purpose of the present invention is to provide an osmanthus essential oil and its application in the preparation of a natural children's shampoo for removing dandruff, relieving itching and controlling oiliness.

[0005] The present invention provides an osmanthus essential oil, which is prepared by the following steps: Pick fresh osmanthus flowers at the early flowering stage of silver osmanthus, remove the flower stalks and impurities, and only keep the flowers. Mix the flowers with the enzyme solutions of β - glucosidase and linalool synthase for enzymatic hydrolysis to obtain hydrolyzed osmanthus flowers; the material - liquid ratio of the fresh osmanthus flowers to the enzyme solutions is 1g: 20 - 30mL; use ethanol as the solvent, and perform reflux extraction on the hydrolyzed osmanthus flowers to obtain osmanthus essential oil.

[0006] Preferably, the enzyme activity ratio of β - glucosidase to linalool synthase in the enzyme solution is 1 - 2:1 - 2; the total enzyme activity in the enzyme solution is 200 - 400U.

[0007] Preferably, the conditions for enzymatic hydrolysis are 35°C - 45°C; the time for enzymatic hydrolysis is 1 - 3h.

[0008] Preferably, the material - liquid ratio of the hydrolyzed osmanthus flowers to ethanol is 1g:8 - 12mL, the conditions for reflux extraction are 50°C - 70°C, the time is 2 - 4h, and the number of refluxes is 2 - 4 times.

[0009] The present invention also provides an application of the osmanthus essential oil described in the above technical solution in the preparation of a natural children's shampoo for anti-dandruff, itching relief and oil control.

[0010] The present invention also provides a natural children's shampoo for anti-dandruff, itching relief and oil control. By mass percentage, the children's shampoo is composed of the following components: 5% - 10% nanoemulsion, 10% - 15% cocamidopropyl betaine, 8% - 12% alkyl polyglycoside, 3% - 5% glycerol, 1% - 3% polydimethylsiloxane, 0.1% - 0.2% sodium benzoate and the balance of water; By mass percentage, the nanoemulsion contains 2% - 5% of the osmanthus essential oil described in the above technical solution, 5% - 10% coconut oil, 5% - 10% camellia oil, 2% - 5% PEG-30 phytosterol, 1% - 3% sophorolipid, 5% - 10% propylene glycol and the balance of water.

[0011] The present invention also provides a preparation method of the children's shampoo described in the above technical solution, including the following steps: Heat and magnetically stir osmanthus essential oil, coconut oil and camellia oil at 60°C - 80°C and 500 - 1000 rpm for 10 - 15 min to obtain an oil phase; Mix PEG-30 phytosterol, sophorolipid, propylene glycol and water, and stir at 60°C - 80°C and 500 - 1000 rpm until completely dissolved to obtain an aqueous phase; Add the oil phase to the aqueous phase, disperse at 12000 - 18000 rpm for 2 - 5 min, and then perform high-pressure homogenization treatment to obtain a nanoemulsion; Mix cocamidopropyl betaine and alkyl polyglycoside with water, and stir until completely dissolved to obtain a surfactant solution; Mix glycerol and polydimethylsiloxane with the surfactant solution, then add the nanoemulsion, stir evenly, add sodium benzoate, and adjust the pH value of the mixture to 5.5 - 6.5 with a weak acid, and stir evenly to obtain the children's shampoo.

[0012] Preferably, the conditions of the high-pressure homogenization are 80 MPa and 6 cycles.

[0013] Preferably, the weak acid is acetic acid or citric acid.

[0014] Advantages of the present invention: By optimizing the extraction process of osmanthus essential oil and adopting the method of enzymatic pretreatment combined with reflux extraction, the present invention significantly improves the extraction efficiency and the content of active ingredients of osmanthus essential oil. The enzymatic hydrolysis step can effectively decompose the cell wall in osmanthus and release more active ingredients, and the reflux extraction further ensures the efficient extraction of these ingredients. Compared with the traditional extraction method, the extraction process of the present invention can better retain the natural active ingredients in osmanthus essential oil, such as linalool, β-glucoside, etc., thereby improving its nursing effect on the scalp.

[0015] The children's shampoo provided by the present invention has the effects of removing dandruff and relieving itching, and controlling oil. It can gently clean the scalp, remove excess oil and residues, and at the same time does not damage the sebum film, being suitable for sensitive scalps. The osmanthus essential oil therein has remarkable effects of removing dandruff and relieving itching, and controlling oil, and can effectively improve the health condition of children's scalps. Applying the osmanthus essential oil to the children's shampoo can provide gentle and efficient scalp care for children. Compared with traditional shampoos, the children's shampoo of the present invention can not only clean the hair, but also significantly reduce problems such as scalp itching, dandruff and excessive oil secretion, and at the same time avoid the stimulation of chemical components to children's scalps.

[0016] In addition to osmanthus essential oil, natural ingredients such as coconut oil, camellia oil, PEG-30 phytosterol, and sophorolipid are also added. These ingredients act synergistically with the osmanthus essential oil to further enhance the care effect of the shampoo.

[0017] The medium-chain fatty acids (such as lauric acid) contained in coconut oil have relatively small molecules, can penetrate deep into the hair cuticle, dissolve the oil, silicone residue and dirt accumulated on the scalp, and play the role of a natural "cleaner"; the main component of hair is keratin, and coconut oil can reduce the loss of keratin caused by the penetration of water during shampooing, and can also form a protective layer on the hair surface to prevent water evaporation, helping to relieve problems such as scalp dryness and itching.

[0018] Camellia oil, also known as camellia seed oil, is rich in active ingredients such as oleic acid, vitamin E, tea polyphenols, and squalene, and can provide deep nourishment and repair for hair and scalp; it contains more than 80% of highly permeable oleic acid, which is similar to the hair cuticle structure, can quickly penetrate the hair cuticle, fill the damaged parts, and repair split ends and breaks; vitamin E and tea polyphenols can neutralize free radicals and reduce oxidative damage caused by ultraviolet rays, perming, dyeing, hair drying, etc. The saponin contained therein also has good biological activity, with properties such as antioxidant, emulsifying, lubricating, and bactericidal. After shampooing, it can inhibit the generation of Malassezia, effectively remove dandruff, prevent the regeneration of dandruff, and make the hair smooth and easy to manage.

[0019] The production process of the present invention includes high-pressure homogenization treatment and nanoemulsion technology, which can evenly disperse osmanthus essential oil and other natural ingredients in the shampoo to form a stable nanoemulsion; it not only improves the stability of the product, but also enhances the permeability and absorption effect of the essential oil, enabling it to better act on the scalp and exert its care effect.

[0020] The children's shampoo of the present invention completely uses natural ingredients and mild chemical raw materials, avoiding the use of common chemical synthetic preservatives, fragrances and pigments in traditional shampoos. By using natural preservatives (such as sodium benzoate) and weak acids (such as acetic acid or citric acid) to adjust the pH value, the shampoo of the present invention is not only safe and non-irritating, but also has good environmental performance, meeting the needs of modern consumers for natural, safe and environmentally friendly products. Detailed Embodiments

[0021] To further illustrate the present invention, the solutions provided by the present invention will be described in detail below in combination with embodiments, but they cannot be construed as limiting the protection scope of the present invention.

[0022] In the embodiments of the present invention, β-glucosidase is purchased from Shanghai Yuanye Bio-Technology Co., Ltd., and the CAS number is 9001-22-3; linalool synthase is purchased from Creative Enzymes, and the CAS number is 254993-26-5.

[0023] Example 1 Fresh osmanthus flowers are picked at the early flowering stage of Osmanthus fragrans 'Latifolius', the flower stalks and impurities are removed, and only the flowers are retained. The flowers are mixed with the enzyme solutions of β-glucosidase and linalool synthase (the enzyme activity of β-glucosidase is 100 U / L, and the enzyme activity of linalool synthase is 100 U / L) according to a material-liquid ratio of 1 g:20 mL, and enzymolyzed at 40 °C for 2 h to obtain enzymolyzed osmanthus flowers; using ethanol as a solvent, the enzymolyzed osmanthus flowers are reflux-extracted at 60 °C for 3 h, the material-liquid ratio of the enzymolyzed osmanthus flowers to ethanol is 1 g:8 - 12 mL, and the number of refluxes is 3 times. After the extraction is completed, osmanthus essential oil is obtained.

[0024] Comparative Example 1 The difference from Example 1 is that β-glucosidase and cellulase are used, specifically as follows: Fresh osmanthus flowers are picked at the early flowering stage of Osmanthus fragrans 'Latifolius', the flower stalks and impurities are removed, and only the flowers are retained. The flowers are mixed with the enzyme solutions of β-glucosidase and cellulase (the enzyme activity of β-glucosidase is 100 U / L, and the enzyme activity of cellulase is 100 U / L) according to a material-liquid ratio of 1 g:20 mL, and enzymolyzed at 40 °C for 2 h to obtain enzymolyzed osmanthus flowers; using ethanol as a solvent, the enzymolyzed osmanthus flowers are reflux-extracted at 60 °C for 3 h, the material-liquid ratio of the enzymolyzed osmanthus flowers to ethanol is 1 g:8 - 12 mL, and the number of refluxes is 3 times. After the extraction is completed, osmanthus essential oil is obtained.

[0025] Comparative Example 2 The difference from Example 1 is that only β-glucosidase is used, specifically as follows: Fresh osmanthus flowers are picked at the early flowering stage of Osmanthus fragrans 'Latifolius', the flower stalks and impurities are removed, and only the flowers are retained. The flowers are mixed with the enzyme solution of β-glucosidase (the enzyme activity of β-glucosidase is 200 U / L) according to a material-liquid ratio of 1 g:20 mL, and enzymolyzed at 40 °C for 2 h to obtain enzymolyzed osmanthus flowers; using ethanol as a solvent, the enzymolyzed osmanthus flowers are reflux-extracted at 60 °C for 3 h, the material-liquid ratio of the enzymolyzed osmanthus flowers to ethanol is 1 g:8 - 12 mL, and the number of refluxes is 3 times. After the extraction is completed, osmanthus essential oil is obtained. [[ID=2,7]]

[0026] Test Example 1 Taking the osmanthus essential oils prepared in Example 1, Comparative Example 1, and Comparative Example 2 as samples, the content of antibacterial substances in the osmanthus essential oil samples was analyzed using gas chromatography-mass spectrometry (GC-MS). The DPPH radical scavenging rate (%), reducing power (FRAP value), and total phenol content (mg GAE / g) of the osmanthus essential oil samples were measured, and the results are shown in Table 1.

[0027] Table 1 Results of the content of antibacterial substances and antioxidant properties in osmanthus essential oil samples

[0028] As can be seen from Table 1, the osmanthus essential oil prepared by enzymatically hydrolyzing osmanthus with β-glucosidase and linalool synthase in the present invention has a higher content of antibacterial substances and stronger antioxidant ability. Compared with the comparative examples, the linalool content and antioxidant activity in the osmanthus essential oil of the examples are significantly improved, indicating that β-glucosidase and linalool synthase have a synergistic effect, significantly increasing the content of active ingredients in the osmanthus essential oil.

[0029] Test Example 2 Nanofluids were prepared by designing different groups of formulations (see Table 2) and made into children's shampoo in the following manner.

[0030] Table 2 Experimental design of nanofluid formulations

[0031] The oil was heated and magnetically stirred at 65 °C and 1000 rpm for 10 min to obtain the oil phase; the emulsifier, cosolvent, and water were mixed and stirred at 65 °C and 1000 rpm until completely dissolved to obtain the water phase; the oil phase was added to the water phase, dispersed at 15000 rpm for 3 min, and then subjected to high-pressure homogenization treatment to obtain the nanofluid.

[0032] 12 parts of cocamidopropyl betaine and 10 parts of alkyl glycoside were mixed with 65 parts of water and stirred until completely dissolved to obtain the surfactant solution; 3 parts of glycerol and 2 parts of polydimethylsiloxane were mixed with the surfactant solution, then 8 parts of nanofluid were added, stirred evenly, 0.1 part of sodium benzoate was added, and the pH value of the mixture was adjusted to 5.5 - 6.5 with citric acid and stirred evenly to obtain the children's shampoo.

[0033] The detergency of each group of shampoo was measured with reference to the artificial sebum removal rate, and the moisturizing property of each group was detected by the transepidermal water loss test (TEWL). The results are shown in Table 3.

[0034] Table 3 Evaluation of the detergency and moisturizing properties of shampoo

[0035] As can be seen from Table 3, the shampoos prepared with the formulations of Group A and Group D have the strongest detergency. However, compared with them, the TEWL of Group A is lower, indicating that the formulation of Group A has better moisturizing properties.

[0036] Using the oil formulation of Group A above, children's shampoos were prepared in the following two ways.

[0037] Group A1: The oil was heated and magnetically stirred at 65 °C and 1000 rpm for 10 min to obtain an oil phase; PEG-30 phytosterol, sophorolipid, propylene glycol and water were mixed and stirred at 65 °C and 1000 rpm until completely dissolved to obtain an aqueous phase; the oil phase was added to the aqueous phase, dispersed at 15000 rpm for 3 min, and then subjected to high-pressure homogenization treatment to obtain a nanoemulsion. 12 parts of cocamidopropyl betaine and 10 parts of alkyl glycoside were mixed with 65 parts of water and stirred until completely dissolved to obtain a surfactant solution; 3 parts of glycerol and 2 parts of polydimethylsiloxane were mixed with the surfactant solution, then 10 parts of nanoemulsion were added, stirred evenly, 0.1 part of sodium benzoate was added, and the pH value of the mixture was adjusted to 5.5 - 6.5 with citric acid and stirred evenly to obtain a children's shampoo.

[0038] Group A2: 4 parts of osmanthus essential oil, 6 parts of coconut oil and 5 parts of camellia oil were mixed evenly to obtain 15 parts of an oil phase solution; 12 parts of cocamidopropyl betaine and 10 parts of alkyl glycoside were mixed with 65 parts of water and stirred until completely dissolved to obtain a surfactant solution; 3 parts of glycerol and 2 parts of polydimethylsiloxane were mixed with the surfactant solution, then 10 parts of oil phase solution were added, stirred evenly, 0.1 part of sodium benzoate was added, and the pH value of the mixture was adjusted to 5.5 - 6.5 with citric acid and stirred evenly to obtain a children's shampoo.

[0039] According to the method recorded in (Chen Yue, Zhang Hongqiang, Wang Jiahui, Zhang Jiamei, Cao Fumin. Study on the antidandruff and antipruritic properties of selenium disulfide shampoo [J]. China Cleaning Products Industry, 2024(3): 41 - 45), the antidandruff and antipruritic properties of the shampoos of Group A1 and Group A2 were evaluated, with 3 parallels in each group, and the results are shown in Table 4.

[0040] Table 4 Antifungal results of Malassezia furfur

[0041] As can be seen from Table 4, the diameter of the inhibition zone of the shampoo in Group A1 is significantly higher than that in Group A2, indicating that the scheme of first preparing osmanthus essential oil into a nanoemulsion and then preparing the shampoo in the present invention can significantly improve its inhibitory effect on Malassezia furfur, and thus effectively reduce the dandruff and oil accumulation caused by Malassezia furfur.

[0042] Recruit 30 volunteers with scalp sebum secretion problems and require the subjects to use the shampoo in Group A1 every day during the trial, 3 to 4 times a week. Measure the scalp sebum secretion amount using a sebum meter on the day before using the shampoo, and on the 1st, 3rd, and 7th days after using the shampoo. The results are shown in Table 5.

[0043] Table 5 Scalp Sebum Secretion Amount

[0044] As can be seen from Table 5, using the shampoo provided by the present invention can effectively reduce the scalp sebum secretion amount, and the osmanthus essential oil provided by the present invention has a good oil control effect after being prepared into shampoo.

[0045] Although the above embodiments have described the present invention in detail, they are only a part of the embodiments of the present invention, not all of them. People can also obtain other embodiments based on these embodiments without creative efforts, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A kind of osmanthus essential oil, characterized in that, The osmanthus essential oil is prepared by the following steps: Pick fresh osmanthus flowers at the early flowering stage of silver osmanthus, remove the flower stalks and impurities, and only keep the flowers. Mix the flowers with the enzyme solutions of β-glucosidase and linalool synthase for enzymatic hydrolysis to obtain hydrolyzed osmanthus flowers. The material-liquid ratio of the fresh osmanthus flowers to the enzyme solutions is 1 g: 20 - 30 mL; Use ethanol as a solvent to reflux and extract the hydrolyzed osmanthus flowers to obtain osmanthus essential oil.

2. The essential oil according to claim 1, characterized in that, The enzyme activity ratio of β-glucosidase to linalool synthase in the enzyme solutions is 1 - 2: 1 - 2; the total enzyme activity in the enzyme solutions is 200 - 400 U.

3. The essential oil according to claim 1, characterized in that, The conditions for the enzymatic hydrolysis are 35°C - 45°C; the time for the enzymatic hydrolysis is 1 - 3 h.

4. The essential oil according to claim 1, characterized in that, The material-liquid ratio of the hydrolyzed osmanthus flowers to ethanol is 1 g: 8 - 12 mL. The conditions for the reflux extraction are 50°C - 70°C, the time is 2 - 4 h, and the number of refluxes is 2 - 4 times.

5. Use of the osmanthus essential oil according to claim 1 in the preparation of a natural children's shampoo for removing dandruff, relieving itching and controlling oiliness.

6. A natural children's shampoo for anti-dandruff, itching relief and oil control, characterized in that, By mass percentage, the children's shampoo is composed of the following components: 5% - 10% nanoemulsion, 10% - 15% cocamidopropyl betaine, 8% - 12% alkyl polyglycoside, 3% - 5% glycerol, 1% - 3% polydimethylsiloxane, 0.1% - 0.2% sodium benzoate and the balance of water; By mass percentage, the nanoemulsion contains 2% - 5% of the osmanthus essential oil according to claim 1, 5% - 10% coconut oil, 5% - 10% camellia oil, 2% - 5% PEG-30 phytosterol, 1% - 3% sophorolipid, 5% - 10% propylene glycol and the balance of water.

7. The preparation method of the children's shampoo according to claim 6, characterized in that, It includes the following steps: Heat and magnetically stir the osmanthus essential oil, coconut oil and camellia oil at 60°C - 80°C and 500 - 1000 rpm for 10 - 15 min to obtain an oil phase; mix PEG-30 phytosterol, sophorolipid, propylene glycol and water, and stir at 60°C - 80°C and 500 - 1000 rpm until completely dissolved to obtain an aqueous phase; add the oil phase to the aqueous phase, disperse at 12000 - 18000 rpm for 2 - 5 min, and then perform high-pressure homogenization treatment to obtain a nanoemulsion; Mix cocamidopropyl betaine and alkyl polyglycoside with water and stir until completely dissolved to obtain a surfactant solution; Mix glycerol and polydimethylsiloxane with the surfactant solution, then add the nanoemulsion, stir evenly, add sodium benzoate, and use a weak acid to adjust the pH value of the mixture to 5.5 - 6.5, and stir evenly to obtain a children's shampoo.

8. The preparation method according to claim 7, characterized in that, The conditions for the high-pressure homogenization are 9. The preparation method according to claim 7, characterized in that, ​

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