44 results about "Isomaltose" patented technology

The present application belongs to the technical field of daily chemical preparation, and particularly relates to a gynecological gel for inhibiting bacteria, regulating microecological balance and tightening vagina and a preparation method thereof. The gynecological gel comprises the following raw materials in mass fractions: 0.5-1 parts of carbomer, 0.04-0.09 parts of sodium hydroxide, 1-3 parts of collagen, 0.01-0.06 parts of sodium hyaluronate, 0.5-1.5 parts of isomaltooligosaccharide, 2-5 parts of propylene glycol, 0.05-0.3 parts of hydroxybenzoic acid methyl ester, 0.02-0.15 parts of hydroxyphenyl ethyl ester, 0.1-1.5 parts of hydrogenated lecithin, 1-6 parts of 1,3-butanediol, 3-5 parts of glycerol, 0.5-1 part of xanthan gum and 70-90 parts of water. The present application uses buckwheat filtrate to prepare collagen, and the active ingredients in the buckwheat filtrate can penetrate the cell membrane of pathogenic bacteria to play an antibacterial role; the collagen can repair the vaginal mucosa barrier and reduce the adhesion and invasion of pathogenic bacteria; therefore, after being compounded with the raw materials such as carbomer, sodium hydroxide, sodium hyaluronate, isomaltooligosaccharide and propylene glycol, the multiple components can synergistically promote the synthesis and repair of collagen fibers and improve the elasticity of tissues.

The invention discloses a copper-molybdenum bulk concentrate flotation separation combined inhibitor and application thereof, and belongs to the technical field of mineral flotation separation. The combined inhibitor is formed by mixing isomaltose and sodium hexametaphosphate. The method for using the combined inhibitor for flotation separation of the copper-molybdenum bulk concentrate specifically comprises the steps that the copper-molybdenum bulk concentrate is subjected to scrubbing, reagent removal and pulping; a combined inhibitor, a collecting agent and a foaming agent are sequentially added into the ore pulp, primary roughing is conducted, and roughing concentrate and roughing tailings are obtained; the roughing concentrate is subjected to three times of concentration, a combined inhibitor is supplemented in each time of concentration, and copper concentrate is obtained; and the roughing tailings are subjected to two times of scavenging, the collecting agent is supplemented in each time of scavenging, and molybdenum concentrate is obtained. According to the method, the floatability difference of the copper ore and the molybdenum ore is enlarged by inhibiting molybdenum and floating copper, the use of high-toxicity agents can be reduced, meanwhile, copper can be efficiently recycled, the grade of molybdenum concentrate can be guaranteed, and good environmental compatibility and industrial application prospects are achieved.

The invention belongs to the technical field of preparation of isomaltooligosacharide, and particularly relates to a high-panose material as well as a preparation method and application thereof. According to the catalytic characteristics of the glucoside transferase, raw materials with specific components are selected, and product guidance is realized by controlling the reaction process. When the maltose content in the raw materials is higher (more than or equal to 80%), the reaction preferentially generates the panose, and meanwhile, the generation of other components such as isomaltose or pantetraose is inhibited, so that the high-purity panose is obtained, the utilization rate of the raw materials is continuously improved, and the reaction time is shortened. In addition, the preparation method provided by the invention overcomes the problems of wide component distribution, low water activity and easy moisture absorption of the isomaltooligosacharide.

The invention discloses a dietary therapy formula for preventing senile dementia, improving memory, repairing a conducted nervous system and dredging damaged brain nerves, and belongs to the technical field of dietary therapy health care. According to the formula, functional nutritional ingredients, bioactive peptides, medicinal and edible extracts and compound probiotics are taken as cores, and the formula is prepared through scientific proportioning; the health-care food specifically comprises xylitol, isomaltooligosacharide, N-acetylneuraminic acid, phosphatidylserine, bovine brain peptide, walnut peptide, shark peptide, acer truncatum seed oil microcapsule powder, DHA algal oil microcapsule powder, spina date seeds, tea theanine, gamma-aminobutyric acid, Belgian imported inulin, magnesium stearate, and 16 optional specific probiotics and metabiotics. The formula follows a multi-dimensional synergistic principle of nutrition supply-nerve repair-function regulation-absorption synergy, the core component can penetrate through a blood brain barrier, supplement nerve repair raw materials, repair damaged nerve cells and myelin sheaths and regulate neurotransmitter balance, and the probiotics indirectly assist nerve maintenance through intestinal tract-nerve axis, so that the composition has the effects of promoting blood circulation and promoting blood circulation. The synergistic effects of preventing senile dementia, improving memory and repairing and dredging a nervous system are achieved; the raw materials are food-grade safe components, have no side effects, can be prepared into powder, tablet candies and other forms, are convenient to eat and suitable for long-term conditioning, and solve the problems of single component, insufficient repair pertinence and low absorption efficiency of existing nerve care products.

The invention relates to the technical field of food processing, in particular to a functional oligosaccharide-containing glycolipid double-control composite tablet candy, which is prepared from the following raw materials: functional oligosaccharide, a lipid control component, a sweet base, microcrystalline cellulose, magnesium stearate, monoglyceride and beta cyclodextrin, the functional oligosaccharide is selected from one or more of fructo-oligosaccharide, isomaltooligosaccharide and galactooligosaccharide; the fat control component is selected from one or more of phytosterol ester and conjugated linoleic acid glyceride; the sweet base material comprises maltitol and isomaltulose; the raw materials can also comprise a mulberry leaf extract. According to the invention, a compound system of the functional oligosaccharide, the special fat control component and the auxiliary sugar control component is adopted, and all the components form a synergistic effect, so that not only can the glycemic index be effectively reduced, but also the fat absorption can be obviously inhibited, the double control of the sugar and the fat in a real sense is realized, and compared with the existing single-function product, the efficacy is more comprehensive and more obvious.

The invention relates to the technical field of tablet candy preparation, in particular to a tablet candy capable of clearing heat, relieving cough and reducing sputum, which is prepared from the following raw materials in parts by weight: 30 to 60 parts of hydrangea powder, 15 to 60 parts of modified sweetening agent, 5 to 12 parts of functional agent, 10 to 25 parts of maltodextrin, 0.32 to 0.61 part of magnesium stearate, 0.05 to 0.2 part of powder essence and 0.09 to 0.2 part of modified menthol. According to the invention, the modified sweetening agent ensures the sweetness of the tablet candy by virtue of the high sweetness of the stevioside, the isomaltooligosacharide improves the sweet taste and reduces the calorie, and meanwhile, the embedding treatment covers the aftertaste of the stevioside; the modified siraitia grosvenorii extract in the functional agent can enhance the effects of moistening the lung to arrest cough, the ultramicro lily powder can synergistically improve the effects of clearing heat and moistening dryness, and the modified purified platycodin assists in reducing phlegm and relieving sore-throat; the modified menthol prolongs the duration of the cool feeling, avoids strong irritation caused by direct use of menthol crystals, and enables the cool feeling to be mildly released in the oral cavity.

The invention belongs to the technical field of fermented food, and particularly relates to an application of a ginseng fermentation liquor of phytobacterium plantarum HCS03-001 in an anti-fatigue product. The preparation method comprises the following steps: adding water into ginseng, extracting twice, combining extracting solutions, adding isomaltooligosacharide, and sterilizing to obtain a fermentation substrate; the preparation method comprises the following steps: carrying out activation culture on plant lactobacillus HCS03-001 to obtain an activated bacterium solution, inoculating a fermentation substrate, carrying out fermentation and centrifugation, and sterilizing the supernate to obtain ginseng fermentation liquor, so as to play a remarkable anti-fatigue role by regulating ATP synthesis and lactic acid metabolism in vivo, the composition can be used for accelerating metabolism recovery after exercise, prolonging exhaustive exercise time, improving skeletal muscle tolerance, reducing exercise muscle injury and the like, and can be applied to preparation of exercise nutrition / anti-fatigue food, health care products or medicines, including products for regulating sex hormone metabolism level and promoting protein synthesis and muscle function recovery. The method has the advantages of safety, high efficiency and wide application range.

The invention relates to a preparation method of high-carboxyl isomaltooligosacharide, and belongs to the technical field of functional sugar preparation. The preparation method comprises the following steps: (1) taking an aqueous solution of isomaltooligosacharide, adjusting the pH value of a reaction system to 7.0-8.0, adding sodium tungstate, dropwise adding hydrogen peroxide into the reaction solution, dropwise adding alkali liquor to maintain the pH value of the reaction system to 7.0-8.0, and stirring and reacting for 3-5 hours at the temperature of 50-60 DEG C; (2) after the reaction is finished, adding acid into the system, adjusting the pH value of the reaction system to 3.0-4.0, and terminating the reaction; and (3) performing filtration-decoloration, intelligent response ion exchange, concentration and drying on the reaction system to obtain the high-carboxyl isomaltooligosacharide. The preparation method disclosed by the invention can effectively avoid the problems of unstable structure, degradation and deterioration and the like of isomaltooligosacharide in a high-temperature alkaline environment, and meanwhile, the carboxylation reaction efficiency can be remarkably improved.

This invention discloses a urinary metabolic biomarker composition for early lung cancer diagnosis and its application. The urinary metabolic biomarker composition for early lung cancer diagnosis includes ethanolamine, catechol, isomaltose, and nicotinamide. The urinary metabolic biomarker composition provided by this invention offers rapid detection, high specificity, high sensitivity, and high predictive accuracy for early lung cancer diagnosis. It is non-invasive and non-surgical, facilitating earlier intervention and targeted, individualized precision treatment for patients, thereby improving patient survival rates. Furthermore, the urinary metabolic biomarker composition uses urine samples for early lung cancer diagnosis, which has advantages such as easy collection, low processing costs, and long-term cryopreservation.

The present invention relates to isomaltooligosaccharide, a preparation method therefor, and use thereof. The preparation method comprises the following steps: adding water into starch to prepare starch milk, adding α-amylase, liquefying the mixture, and then adding β-amylase and pullulanase for primary saccharification to obtain a crude maltose liquid; adding a transglucosidase for secondary saccharification to obtain a crude isomaltooligosaccharide liquid; and filtering the crude isomaltooligosaccharide liquid, and carrying out ion exchange, decoloration, and evaporation concentration to obtain the isomaltooligosacharide. According to the preparation method, the saccharide proportion of relatively high polymerization degree (DP4, DP5, DP6-9) in isomaltooligosacharide is significantly improved, so that the activity of the isomaltooligosaccharide serving as prebiotics is improved, the transparency of the isomaltooligosaccharide is effectively improved, and the color value of the isomaltooligosaccharide is reduced, thereby reducing the application limitation of the isomaltooligosacharide in product formulas of personal care products (skin, hair, scalp, oral cavity), food, pet care products, and the like.

The application provides a water-soluble dietary fiber and application of the water-soluble dietary fiber in preparation of a medicine for promoting metabolism of ellagic acid into urolithin A and a medicine composition, and belongs to the technical field of biological medicines.The water-soluble dietary fiber provided by the application comprises at least two components: gellan gum, guar gum, carrageenan, fructooligosaccharide, xylooligosaccharide, isomaltooligosaccharide, pectin, beta-glucan, polydextrose, sodium alginate, gum arabic, resistant dextrin, xanthan gum, tragacanth gum and inulin.In the application, the water-soluble dietary fiber and ellagic acid in different combinations are compounded to feed mice, and the average content of urolithin A in the feces of the mice can be significantly improved.It can be seen that the water-soluble dietary fiber or the combination of the water-soluble dietary fiber and ellagic acid can improve the metabolic type of the body ellagic acid and increase the content of urolithin A, thereby providing a new means for the treatment of diabetes, obesity, aging and central nervous system diseases.

The invention belongs to the technical field of fermented food, and particularly relates to a method for preparing ginseng fermentation liquor through fermentation of plant lactobacillus HCS03-001. The method provided by the invention comprises the following steps: combining a ginseng enzymolysis extracting solution and a second extracting solution, or combining a first extracting solution and the second extracting solution of ginseng, adding isomaltooligosacharide, and sterilizing to obtain a fermentation substrate; inoculating a bacterium solution of the plant lactobacillus HCS03-001 into the culture medium for activation culture to obtain an activated bacterium solution; inoculating the activated bacterial liquid into a fermentation substrate according to the inoculation amount of 4-6% v / v, centrifuging after fermentation, and sterilizing supernate to obtain the ginseng fermentation liquid. The ginseng fermentation liquor prepared by the method provided by the invention is rich in Rg3 and other ginseng rare saponins, the problems of insufficient dissolution of active ingredients and extremely low content of the rare saponins in traditional extraction are solved, and in addition, dry ingredients (Rg1 and Re) which can cause dryness-heat feeling, parched mouth and scorched tongue, annoyance and other symptoms of people in the ginseng can be reduced.

Even among low-alcohol beverages, there is a demand for beer-flavored drinks that possess the sweetness and crisp aftertaste suitable for beer-flavored beverages. [Solution] A beer-flavored beverage having an alcohol content of 6.0 (v / v)% or less, a ratio of alcohol content (unit: (v / v)%) to isomaltose (A) content (unit: g / 100mL) [alcohol content / (A)] of 45.0 or less, and a total content of monosaccharides or disaccharides consisting of glucose (C1), fructose (C2), sucrose (D1), and maltose (D2) of 0.10 g / 100mL or less.

The present invention provides a lyophilized composition containing tolvaptan sodium phosphate and a preparation method for the lyophilized composition. One or more of maltitol, raffinose, dextran, stachyose, arabinogalactan, fructooligosaccharide, galactooligosaccharide, isomaltooligosaccharide, polyethylene glycol, xylitol, or sorbitol is / are used as a lyoprotectant, so that the problem in the prior art that lyophilized compositions containing tolvaptan sodium phosphate are difficult to satisfy both high stability and high clarity; provided a tolvaptan sodium phosphate-containing composition which satisfies both high stability and high clarity, has a high dissolution rate and is easy to apply, reducing generation of impurities; in addition, in the preparation and long-term storage process, better stability is achieved, the opalescence and turbidity of liquid medicine during reconstitution and redissolution are avoided, dissolution is easier, and safe application of the product is facilitated.

The present invention relates to the use of monoester glycolipids or mixtures of monoester glycolipids in laundry detergent compositions. The monoester glycolipids or mixtures of monoester glycolipids include a carbohydrate fraction selected from the group consisting of maltose, cellobiose, trehalose, isomaltulose, lactulose, isomaltose and mixtures thereof.

The invention discloses sugar-free high-functionality isomaltooligosacharide and a preparation method thereof, and belongs to the technical field of functional sugar manufacturing. The invention discloses a preparation method of sugar-free high-functionality isomaltooligosacharide, which comprises the following steps of: converting starch into coarse ultrahigh maltose syrup by using high-temperature-resistant alpha-amylase, barley-beta-amylase and pullulanase, further refining the coarse ultrahigh maltose syrup through deslagging, decolorizing and ion exchange, adding glucosylase, and carrying out high-temperature hydrolysis to obtain the sugar-free high-functionality isomaltooligosacharide. And then purifying the crude isomaltooligosacharide by adopting a nanofiltration membrane, and removing glucose in the sugar liquid. The content of glucose and maltose in the prepared isomaltose hypgather is lower than 0.5%, the isomaltose hypgather meets the requirements of the current market for healthy and sugar-free high-functional products, the content of effective sugar IG < 2 + > P + IG3 is larger than or equal to 90%, and the product has higher functional components which are two times that of the current market products.

The invention discloses a fiber-containing pre-digested full-nutrient formula food and a preparation method thereof, and relates to the technical field of full-nutrient formula foods. The preparation method of the fiber-containing pre-digested full-nutrient formula food is simple to operate, the nutrient loss in the production process is less, the nutrient index control is accurate, and the fiber-containing pre-digested full-nutrient formula food is suitable for large-scale industrial production; the components are comprehensive and rich and are easy to digest and absorb by a body, soluble dietary fibers such as fructo-oligosaccharide, isomaltooligosacharide and xylooligosaccharide are especially added, and the intestinal burden of a patient is reduced to the maximum extent on the basis of ensuring reasonable diet of the patient through the synergistic effect of all the components, so that the health-care food has a good health-care effect. The rehabilitation of diseases of patients with gastrointestinal function insufficiency or partial deficiency states such as acute and chronic pancreatitis, diarrhea and inflammatory intestinal diseases can be promoted.

This invention relates to the field of biotechnology, and more particularly to a nicotinamide microcapsule, its preparation method, and its uses. The preparation method of the nicotinamide microcapsule includes the following steps: mixing gelatin and water and then heating the mixture to obtain a gelatin solution; under constant temperature conditions of 38-45℃, sequentially dissolving L-α-glycine phosphate, sodium alginate, isomaltooligosaccharide, nicotinamide, and vitamin C in the gelatin solution to obtain a mixed solution; adjusting the pH of the mixed solution and then continuously stirring to obtain the nicotinamide microcapsule. This nicotinamide microcapsule exhibits good encapsulation effect and high encapsulation rate, demonstrating excellent encapsulation ability for nicotinamide, effectively improving the absorption efficiency of nicotinamide, and enabling nicotinamide to be converted into more NAD+. + This leads to the production of more ATP, thereby increasing the bioavailability of nicotinamide.

The invention provides a composition for reducing the age of the intestinal tract, an oral composition formed by the composition and application, the composition for reducing the age of the intestinal tract comprises probiotics and prebiotics, and the probiotics are bifidobacteria; the prebiotics comprise two or more than two of polydextrose, isomaltooligosacharide and galactooligosaccharide. The composition can effectively influence and change the composition of intestinal bacteria of a body, promote the abundance of core characteristic bacteria representing young intestinal tracts to be increased and inhibit the growth of bacteria representing aging intestinal tracts, and people tests prove that the composition can effectively change the intestinal flora structure tending to be aged, reduce the age of the intestinal tracts and improve the immunity of the intestinal tracts. The intestinal tract becomes young.

This invention provides a fecal microbial freeze-drying protective solution. Through the synergistic effect of components such as phosphorylcholine polymer, glycerol, trehalose, ergothioneine, L-cysteine, melibiose, isomaltooligosaccharide, and stachydrine, it effectively reduces the damage to bacterial activity during the freeze-drying process, improves the stability of freeze-drying preservation, and enhances the ability to maintain bacterial metabolic activity. It plays a significant role in maintaining the diversity and functional activity of the bacterial community.

The invention belongs to the field of liver cirrhosis and hepatocellular carcinoma marker detection, and particularly relates to application of a metabolic marker detection reagent in preparation of liver disease diagnosis products. The metabolic marker is a combination of Nicotinamide N-oxide, Pregnandiol 3-O-glucose, Guanidoacetic acid, Glycine, Isomaltose, Picolinic acid, 2-Hydroxycyanic acid, Quinolinic acid, Carnosine, 3-Methyluridine, 3-Methyluridine and Formiminoglumic acid, and the metabolic marker is a combination of the Nicotinamide N-oxide, the Pregnandiol 3-O-glucose acid, the Guanidoacetic acid, the Glycine, the Isomaltose, the Picolinic acid, the 2-Hydroxycyanic acid, the Quinolinic acid, the Carnosine, the 3-Methyluridine, the 3-Methyluridine and the Formiminoglumic acid. The liver disease is liver cirrhosis or hepatocellular carcinoma. The 12 urine metabolism markers are combined to accurately distinguish normal people from patients with liver cirrhosis, and normal people from patients with hepatocellular carcinoma. The kit provides a brand-new and objective biological basis for early diagnosis, treatment and prognosis evaluation of liver cirrhosis and hepatocellular carcinoma.

The invention relates to skin-care anti-aging goat milk powder and a preparation method thereof. By adding the isomaltooligosacharide, the demineralized whey powder, the whey protein powder, the inulin, the compound vitamins, the compound minerals, the calcium carbonate, the triple compound probiotics and the like into the raw goat milk powder, the nutritional ingredients of the milk powder are improved, so that the nutrition is more comprehensive, in addition, the added compound L-carnitine tartrate is beneficial to accelerating fat decomposition, the fat content is reduced, and the fat content is reduced. And the collagen peptide can effectively regulate the intestinal environment, does not cause fat accumulation after being eaten for a long time, has the effects of delaying senescence, nourishing skin, regulating human metabolism and the like, and makes up the market blank. A dry mixing method is adopted for preparation of the product, nutritional ingredients of the product cannot be lost in the production process, and the processing process is simple.

The present application relates to the technical field of sports nutrition food, in particular to a quick-absorption electrolyte sports drink and a preparation method thereof, which solves the problems of low electrolyte absorption efficiency, unbalanced osmotic pressure, easy loss of flavor and single function of existing sports drinks. The drink is based on pure water, and is compounded with a composite electrolyte system, a double-carrier synergistic absorption system, a natural flavor regulator and other raw materials. The composite electrolyte simulates the proportion of human sweat, and the double carrier is made of oligomeric isomaltose and whey protein hydrolysate at a ratio of 5:1. The product is prepared by raw material pretreatment, electrolyte dissolution, double-carrier compounding, low-temperature microcapsule homogenization, pH adjustment and pulse type pasteurization filling. The product can accurately control the osmotic pressure and pH, greatly improve the electrolyte absorption efficiency, stabilize the flavor and have a shelf life of up to 12 months, and is safe and free of harmful additives, suitable for various high-intensity sports scenes and excellent in replenishment effect.

To provide a plaque formation inhibitor that can suppress plaque formation to a high degree. [Solution] The plaque formation inhibitor contains at least one compound selected from cyclodextran and cyclodextran derivatives detected from a culture solution of Bacillus microorganisms using dextran as a substrate and / or a reaction solution of cyclic isomaltose synthase using dextran as a substrate, and at least one compound selected from isopropylmethylphenol, catechin, menthol, specific sugar alcohols (sugar alcohols with antibacterial effects), cetylpyridinium chloride, and berberine.

The invention discloses salt-reduced compound flavor beef jerky and a preparation method thereof. The salt-reduced compound flavor beef jerky is prepared from the following raw materials in percentage by weight: 100 g of fresh beef and 100 g of pickling seasonings, the pickling seasoning is prepared from 1.2 to 1.8 g of table salt, 0.2 to 0.3 g of potassium chloride, 0.3 to 0.5 g of yeast extract, 0.5 to 1.0 g of kelp extract, 0.2 to 0.3 g of shiitake mushroom powder, 1.0 to 1.5 g of trehalose, 0.5 to 1.2 g of isomaltooligosacharide, 0.05 to 0.1 g of liquorice root extract, 0.02 to 0.03 g of rosemary extract, 0.2 to 0.3 g of calcium ascorbate, 1.5 to 2.0 g of compound spice powder, 1.0 to 1.5 mL of ginger concentrated juice and 15 to 20 mL of water. On the premise that the sodium content is remarkably reduced, the comprehensive quality of the product in the aspects of flavor, texture, stability, safety and the like is synchronously guaranteed and even improved.

The invention discloses a preparation method and application of yak milk skin powder. Through the synergistic effect of the flavor embedding agent (such as beta-cyclodextrin) and the water activity regulating agent (such as isomaltooligosacharide) and in combination with the integrated process of staged low-temperature dehydration and nitrogen-filled packaging, the problems that the flavor of the traditional yak dairy product is easy to dissipate, the grease is easy to oxidize and the shelf life is short are essentially solved. The core of the invention lies in that the characteristic flavor substances of the yak milk are effectively locked through molecular inclusion, and oxidation deterioration and microbial growth are significantly inhibited by regulating and controlling the microenvironment of the system. The prepared yak milk skin powder product is excellent in performance, the characteristic flavor retention rate is higher than 90%, the peroxide value is lower than 5 meq / kg, the rehydration property is good, dissolution is rapid, and the yak milk skin powder product has excellent storage stability and convenience. According to the preparation method, the natural nutrition and flavor of the yak milk are reserved to the maximum extent.

An aerated and grained nougat composition free from or low in monosaccharides and disaccharides, the composition including at least about 20% isomalt, by weight of the composition.

The invention provides a confectionery composition comprising: a) at least one fructan; b) at least one isomaltose oligosaccharide (IMO); and c) at least one further soluble dietary fibre ingredient.

The invention provides a multi-component difference substance for identifying acacia honey and rape honey and an acquisition method thereof, and relates to the technical field of honey quality and authenticity identification. The method comprises the following steps: carrying out component identification on black locust honey and rape honey by adopting 12 sugars and 20 amino acids, establishing sugar and amino acid maps in black locust honey and rape honey component extracting solutions through UPLC-MS / MS (Ultra Performance Liquid Chromatography-Mass Spectrometry / Mass Spectrometry), and obtaining key difference substances by utilizing a multi-layer perceptron model; the method for jointly identifying the acacia honey and the rape honey by multi-component difference substances including fructose, pinocembriose, glucose, pinocembriose, maltulose, glucopyranosyl sucrose, maltose, isomaltose, gamma-aminobutyric acid, tryptophan, arginine and aspartic acid is established. The method disclosed by the invention is beneficial to clarification of the difference of the chemical maps of the sugar and the amino acid of the high-value black locust honey and the low-value rape honey, has the advantages of simplicity, rapidness, accuracy and reliability, and provides a reliable method for authenticity identification and quality evaluation of the high-value black locust honey.

The invention discloses an enzymatic preparation method of high-proportion long-chain isomaltooligosacharide. According to the method, starch / maltodextrin (with the DE value of 5-10) is taken as a raw material, and the high-proportion long-chain isomaltooligosacharide is prepared through yeast fermentation and purification by virtue of reconstruction of a bonding mode among glycosyl units under composite synergistic catalysis of alpha-amylase, pullulanase, maltotriose producing enzyme and alpha-glucosidase. The purity of the prepared isomaltooligosacharide reaches 95.93%, and the proportion of the long-chain isomaltooligosacharide is 34.12%, which is far higher than the proportion of the long-chain isomaltooligosacharide in the existing commercial products by 5-15%. The long-chain isomaltooligosacharide can remain in the intestinal tract for a long time, so that the long-chain isomaltooligosacharide can exert stronger life benefiting activity and is very concerned. According to the method, the high-purity and high-proportion long-chain isomaltooligosacharide can be prepared, the problems that the isomaltooligosacharide is poor in purity, the proportion of the long-chain isomaltooligosacharide is not high and the like are effectively solved, and the obtained product has wide application in the food industry.