64 results about "Isomaltose" patented technology

The invention belongs to the technical field of dairy product processing, and discloses camel milk composite nutrition powder beneficial to bone health, the camel milk composite nutrition powder is composed of the following raw materials by weight: 70%-75% of whole camel milk powder, 5%-15% of whey protein, 5%-20% of isomaltooligosacharide, 1%-10% of inulin, 0.02%-8% of milk mineral salt, 0.02%-5% of hydrolyzed yolk powder, and the balance water. The oral liquid is prepared from the following components in percentage by weight: 0.01%-0.5% of colostrum basic protein, 0.01%-1% of cartilage powder containing type II collagen, 0.01%-0.1% of N-acetylglucosamine, 0.01%-0.02% of sodium hyaluronate, 0.05%-5% of collagen peptide and 0.01%-1% of liquorice extract. According to the invention, a multi-dimensional health system is constructed by scientifically compounding a plurality of key components: milk mineral salt, colostrum basic protein and hydrolyzed egg yolk powder synergistically promote bone mineral density increase and bone metabolism balance; the II-type collagen cartilage powder, the N-acetylglucosamine and the sodium hyaluronate are combined to realize joint protection and skin repair; the six vitamins and the four minerals can accurately regulate and control the nutrition metabolism of the middle-aged and elderly people, especially can strengthen calcium absorption and oxidation resistance, and can significantly improve the bone health and improve the skin state.

The present application belongs to the technical field of daily chemical preparation, and particularly relates to a gynecological gel for inhibiting bacteria, regulating microecological balance and tightening vagina and a preparation method thereof. The gynecological gel comprises the following raw materials in mass fractions: 0.5-1 parts of carbomer, 0.04-0.09 parts of sodium hydroxide, 1-3 parts of collagen, 0.01-0.06 parts of sodium hyaluronate, 0.5-1.5 parts of isomaltooligosaccharide, 2-5 parts of propylene glycol, 0.05-0.3 parts of hydroxybenzoic acid methyl ester, 0.02-0.15 parts of hydroxyphenyl ethyl ester, 0.1-1.5 parts of hydrogenated lecithin, 1-6 parts of 1,3-butanediol, 3-5 parts of glycerol, 0.5-1 part of xanthan gum and 70-90 parts of water. The present application uses buckwheat filtrate to prepare collagen, and the active ingredients in the buckwheat filtrate can penetrate the cell membrane of pathogenic bacteria to play an antibacterial role; the collagen can repair the vaginal mucosa barrier and reduce the adhesion and invasion of pathogenic bacteria; therefore, after being compounded with the raw materials such as carbomer, sodium hydroxide, sodium hyaluronate, isomaltooligosaccharide and propylene glycol, the multiple components can synergistically promote the synthesis and repair of collagen fibers and improve the elasticity of tissues.

The invention discloses a copper-molybdenum bulk concentrate flotation separation combined inhibitor and application thereof, and belongs to the technical field of mineral flotation separation. The combined inhibitor is formed by mixing isomaltose and sodium hexametaphosphate. The method for using the combined inhibitor for flotation separation of the copper-molybdenum bulk concentrate specifically comprises the steps that the copper-molybdenum bulk concentrate is subjected to scrubbing, reagent removal and pulping; a combined inhibitor, a collecting agent and a foaming agent are sequentially added into the ore pulp, primary roughing is conducted, and roughing concentrate and roughing tailings are obtained; the roughing concentrate is subjected to three times of concentration, a combined inhibitor is supplemented in each time of concentration, and copper concentrate is obtained; and the roughing tailings are subjected to two times of scavenging, the collecting agent is supplemented in each time of scavenging, and molybdenum concentrate is obtained. According to the method, the floatability difference of the copper ore and the molybdenum ore is enlarged by inhibiting molybdenum and floating copper, the use of high-toxicity agents can be reduced, meanwhile, copper can be efficiently recycled, the grade of molybdenum concentrate can be guaranteed, and good environmental compatibility and industrial application prospects are achieved.

The invention discloses a dextran degradable film and a preparation method thereof, and belongs to the technical field of food processing. The preparation method of the degradable film comprises the following steps: 1) dissolving dextran in a proper amount of deionized water through magnetic stirring at normal temperature to obtain a fully dissolved dextran solution; (2) adding glycerol into the dextran solution, and uniformly mixing; (3) adding an isomaltooligosacharide solution, uniformly mixing, and carrying out ultrasonic degassing; and 4) pouring a certain amount of solution into a polystyrene plate, and drying to obtain the required degradable film. The degradable film prepared by the invention has good mechanical property and biocompatibility, is a novel degradable environment-friendly film, is simple in preparation method, has certain barrier property and transparency, and can be applied to packaging and fresh keeping of food.

The invention belongs to the technical field of preparation of isomaltooligosacharide, and particularly relates to a high-panose material as well as a preparation method and application thereof. According to the catalytic characteristics of the glucoside transferase, raw materials with specific components are selected, and product guidance is realized by controlling the reaction process. When the maltose content in the raw materials is higher (more than or equal to 80%), the reaction preferentially generates the panose, and meanwhile, the generation of other components such as isomaltose or pantetraose is inhibited, so that the high-purity panose is obtained, the utilization rate of the raw materials is continuously improved, and the reaction time is shortened. In addition, the preparation method provided by the invention overcomes the problems of wide component distribution, low water activity and easy moisture absorption of the isomaltooligosacharide.

The invention discloses a dietary therapy formula for preventing senile dementia, improving memory, repairing a conducted nervous system and dredging damaged brain nerves, and belongs to the technical field of dietary therapy health care. According to the formula, functional nutritional ingredients, bioactive peptides, medicinal and edible extracts and compound probiotics are taken as cores, and the formula is prepared through scientific proportioning; the health-care food specifically comprises xylitol, isomaltooligosacharide, N-acetylneuraminic acid, phosphatidylserine, bovine brain peptide, walnut peptide, shark peptide, acer truncatum seed oil microcapsule powder, DHA algal oil microcapsule powder, spina date seeds, tea theanine, gamma-aminobutyric acid, Belgian imported inulin, magnesium stearate, and 16 optional specific probiotics and metabiotics. The formula follows a multi-dimensional synergistic principle of nutrition supply-nerve repair-function regulation-absorption synergy, the core component can penetrate through a blood brain barrier, supplement nerve repair raw materials, repair damaged nerve cells and myelin sheaths and regulate neurotransmitter balance, and the probiotics indirectly assist nerve maintenance through intestinal tract-nerve axis, so that the composition has the effects of promoting blood circulation and promoting blood circulation. The synergistic effects of preventing senile dementia, improving memory and repairing and dredging a nervous system are achieved; the raw materials are food-grade safe components, have no side effects, can be prepared into powder, tablet candies and other forms, are convenient to eat and suitable for long-term conditioning, and solve the problems of single component, insufficient repair pertinence and low absorption efficiency of existing nerve care products.

The invention provides a composition with effects of soothing liver and regulating qi and a preparation method of the composition, the traditional Chinese medicine composition comprises functional components and auxiliary material components, the functional components are traditional Chinese medicine components, and the auxiliary material components are traditional Chinese medicine components. The traditional Chinese medicine composition mainly comprises the following raw materials in parts by mass: 2-8 parts of rose, 2-8 parts of chrysanthemum, 2-8 parts of astragalus membranaceus, 2-8 parts of platycodon grandiflorum, 5-15 parts of honeysuckle, 5-15 parts of oyster, 5-15 parts of poria cocos, 5-15 parts of fingered citron, 5-15 parts of radix angelicae, 2-8 parts of mint, 2-8 parts of pericarpium citri reticulatae, 2-8 parts of peach kernel, 5-15 parts of angelica sinensis, 10-20 parts of dandelion, 10-20 parts of jasmine, 10-20 parts of semen raphani, 2-8 parts of turmeric, 5-15 parts of citron and 10-20 parts of semen cassiae. The auxiliary material component is prebiotic isomaltooligosacharide, and the mass ratio of the prebiotic isomaltooligosacharide to the traditional Chinese medicine component is 1: (4-19). The composition is subjected to traditional Chinese medicine extraction, double-effect concentration, biological enzymolysis-filtration synergy, mixing and blending with prebiotic isomaltooligosacharide, homogenization, constant volume filling, sterilization packaging and other processes to prepare the oral liquid, and the oral liquid has the effects of soothing liver-qi stagnation, promoting qi to activate blood and removing stasis and dissipating binds.

The invention relates to the technical field of food processing, in particular to a functional oligosaccharide-containing glycolipid double-control composite tablet candy, which is prepared from the following raw materials: functional oligosaccharide, a lipid control component, a sweet base, microcrystalline cellulose, magnesium stearate, monoglyceride and beta cyclodextrin, the functional oligosaccharide is selected from one or more of fructo-oligosaccharide, isomaltooligosaccharide and galactooligosaccharide; the fat control component is selected from one or more of phytosterol ester and conjugated linoleic acid glyceride; the sweet base material comprises maltitol and isomaltulose; the raw materials can also comprise a mulberry leaf extract. According to the invention, a compound system of the functional oligosaccharide, the special fat control component and the auxiliary sugar control component is adopted, and all the components form a synergistic effect, so that not only can the glycemic index be effectively reduced, but also the fat absorption can be obviously inhibited, the double control of the sugar and the fat in a real sense is realized, and compared with the existing single-function product, the efficacy is more comprehensive and more obvious.

The invention relates to the technical field of tablet candy preparation, in particular to a tablet candy capable of clearing heat, relieving cough and reducing sputum, which is prepared from the following raw materials in parts by weight: 30 to 60 parts of hydrangea powder, 15 to 60 parts of modified sweetening agent, 5 to 12 parts of functional agent, 10 to 25 parts of maltodextrin, 0.32 to 0.61 part of magnesium stearate, 0.05 to 0.2 part of powder essence and 0.09 to 0.2 part of modified menthol. According to the invention, the modified sweetening agent ensures the sweetness of the tablet candy by virtue of the high sweetness of the stevioside, the isomaltooligosacharide improves the sweet taste and reduces the calorie, and meanwhile, the embedding treatment covers the aftertaste of the stevioside; the modified siraitia grosvenorii extract in the functional agent can enhance the effects of moistening the lung to arrest cough, the ultramicro lily powder can synergistically improve the effects of clearing heat and moistening dryness, and the modified purified platycodin assists in reducing phlegm and relieving sore-throat; the modified menthol prolongs the duration of the cool feeling, avoids strong irritation caused by direct use of menthol crystals, and enables the cool feeling to be mildly released in the oral cavity.

The invention relates to goat milk powder containing colostrum powder and a preparation method thereof. The isomaltooligosacharide, the demineralized whey powder, the whey protein powder, the inulin, the compound vitamins, the compound minerals, the calcium carbonate, the triple compound probiotics and the like are added into the raw goat milk powder, so that the nutritional ingredients of the milk powder are improved, the nutrition is more comprehensive, and in addition, the added bovine colostrum powder has the effects of strengthening physique, promoting development and improving immunity; through reasonable selection and matching of the components, nutritional requirements of teenagers in the growth process can be effectively met, and market vacancy is made up. A dry mixing method is adopted for preparation of the product, the processing process is simple, the cost can be effectively reduced, and production and popularization are facilitated.

The invention belongs to the technical field of fermented food, and particularly relates to an application of a ginseng fermentation liquor of phytobacterium plantarum HCS03-001 in an anti-fatigue product. The preparation method comprises the following steps: adding water into ginseng, extracting twice, combining extracting solutions, adding isomaltooligosacharide, and sterilizing to obtain a fermentation substrate; the preparation method comprises the following steps: carrying out activation culture on plant lactobacillus HCS03-001 to obtain an activated bacterium solution, inoculating a fermentation substrate, carrying out fermentation and centrifugation, and sterilizing the supernate to obtain ginseng fermentation liquor, so as to play a remarkable anti-fatigue role by regulating ATP synthesis and lactic acid metabolism in vivo, the composition can be used for accelerating metabolism recovery after exercise, prolonging exhaustive exercise time, improving skeletal muscle tolerance, reducing exercise muscle injury and the like, and can be applied to preparation of exercise nutrition / anti-fatigue food, health care products or medicines, including products for regulating sex hormone metabolism level and promoting protein synthesis and muscle function recovery. The method has the advantages of safety, high efficiency and wide application range.

The invention provides a sugar control composition with a targeted inhibition function and application of the sugar control composition in health care products, special meals or nutritional meals, and belongs to the technical field of functional foods or health care products. The invention provides a sugar control composition. Comprising chia seeds, polydextrose, isomaltooligosacharide, konjac powder, oat beta glucan, xylooligosaccharide, inulin, green coffee powder, a white kidney bean extract, fermented soybean powder, mulberry leaf and bitter gourd powder, citric acid, sodium citrate, stevioside, white peach concentrated juice, lemon concentrated juice, lime concentrated juice, passion flower concentrated juice, apple concentrated juice and potassium sorbate. The invention also provides a preparation method of the sugar-controlling composition and application of the sugar-controlling composition in preparation of medicines, health-care products, special meals or nutritional meals, and provides a special meal suitable for diabetics to eat. The sugar control composition and the special meal prepared from the sugar control composition can inhibit the biological activity of DPP-IV (peptidase IV) in a targeted mode, and the remarkable sugar control effect is achieved.

The invention relates to a preparation method of high-carboxyl isomaltooligosacharide, and belongs to the technical field of functional sugar preparation. The preparation method comprises the following steps: (1) taking an aqueous solution of isomaltooligosacharide, adjusting the pH value of a reaction system to 7.0-8.0, adding sodium tungstate, dropwise adding hydrogen peroxide into the reaction solution, dropwise adding alkali liquor to maintain the pH value of the reaction system to 7.0-8.0, and stirring and reacting for 3-5 hours at the temperature of 50-60 DEG C; (2) after the reaction is finished, adding acid into the system, adjusting the pH value of the reaction system to 3.0-4.0, and terminating the reaction; and (3) performing filtration-decoloration, intelligent response ion exchange, concentration and drying on the reaction system to obtain the high-carboxyl isomaltooligosacharide. The preparation method disclosed by the invention can effectively avoid the problems of unstable structure, degradation and deterioration and the like of isomaltooligosacharide in a high-temperature alkaline environment, and meanwhile, the carboxylation reaction efficiency can be remarkably improved.

The invention provides a vaginal packing with an antibacterial function and a preparation method and application thereof, and relates to the technical field of vaginal packing, the vaginal packing with the antibacterial function comprises dihydroquercetin, citric acid, galactooligosaccharide, isomaltooligosaccharide, sodium lactate, sodium hyaluronate and chitosan. The dihydroquercetin has a relatively strong antibacterial function; the permeation enhancer chitosan can prolong the residence time of the dihydroquercetin at the absorption part, improve the local concentration gradient and promote the vaginal mucosa to absorb the dihydroquercetin; sodium hyaluronate can improve the solubility and stability of dihydroquercetin, enhance the absorption efficiency of dihydroquercetin, further improve the antibacterial performance, control the release progress, realize slow release, accelerate the permeation of water into the interior and enhance the vaginal filling and imbibition speed. The isomaltooligosacharide and the sodium lactate can be mixed with the chitosan to form an adsorption material which is used for absorbing secretions, providing prebiotics support and adjusting the pH value.

This invention discloses a urinary metabolic biomarker composition for early lung cancer diagnosis and its application. The urinary metabolic biomarker composition for early lung cancer diagnosis includes ethanolamine, catechol, isomaltose, and nicotinamide. The urinary metabolic biomarker composition provided by this invention offers rapid detection, high specificity, high sensitivity, and high predictive accuracy for early lung cancer diagnosis. It is non-invasive and non-surgical, facilitating earlier intervention and targeted, individualized precision treatment for patients, thereby improving patient survival rates. Furthermore, the urinary metabolic biomarker composition uses urine samples for early lung cancer diagnosis, which has advantages such as easy collection, low processing costs, and long-term cryopreservation.

The present invention relates to isomaltooligosaccharide, a preparation method therefor, and use thereof. The preparation method comprises the following steps: adding water into starch to prepare starch milk, adding α-amylase, liquefying the mixture, and then adding β-amylase and pullulanase for primary saccharification to obtain a crude maltose liquid; adding a transglucosidase for secondary saccharification to obtain a crude isomaltooligosaccharide liquid; and filtering the crude isomaltooligosaccharide liquid, and carrying out ion exchange, decoloration, and evaporation concentration to obtain the isomaltooligosacharide. According to the preparation method, the saccharide proportion of relatively high polymerization degree (DP4, DP5, DP6-9) in isomaltooligosacharide is significantly improved, so that the activity of the isomaltooligosaccharide serving as prebiotics is improved, the transparency of the isomaltooligosaccharide is effectively improved, and the color value of the isomaltooligosaccharide is reduced, thereby reducing the application limitation of the isomaltooligosacharide in product formulas of personal care products (skin, hair, scalp, oral cavity), food, pet care products, and the like.

The application provides a water-soluble dietary fiber and application of the water-soluble dietary fiber in preparation of a medicine for promoting metabolism of ellagic acid into urolithin A and a medicine composition, and belongs to the technical field of biological medicines.The water-soluble dietary fiber provided by the application comprises at least two components: gellan gum, guar gum, carrageenan, fructooligosaccharide, xylooligosaccharide, isomaltooligosaccharide, pectin, beta-glucan, polydextrose, sodium alginate, gum arabic, resistant dextrin, xanthan gum, tragacanth gum and inulin.In the application, the water-soluble dietary fiber and ellagic acid in different combinations are compounded to feed mice, and the average content of urolithin A in the feces of the mice can be significantly improved.It can be seen that the water-soluble dietary fiber or the combination of the water-soluble dietary fiber and ellagic acid can improve the metabolic type of the body ellagic acid and increase the content of urolithin A, thereby providing a new means for the treatment of diabetes, obesity, aging and central nervous system diseases.

The invention relates to detoxification type goat milk powder and a preparation method thereof. The isomaltooligosacharide, the demineralized whey powder, the whey protein powder, the inulin, the compound vitamins, the compound minerals, the calcium carbonate, the stachyose, the triple compound probiotics and the like are added into the high-quality raw goat milk, so that the raw goat milk is rich in various nutritional ingredients, and the goat milk powder is obtained after homogeneous mixing and sterilization treatment and spray drying or freeze drying treatment. The prepared goat milk powder is uniform in component and more comprehensive in nutrition, the added stachyose has the effects of regulating intestinal flora, relaxing bowel and maintaining skin elasticity, excretion and detoxification of a human body can be effectively promoted, and the functional effect of the goat milk powder is further improved.

The invention belongs to the technical field of fermented food, and particularly relates to a method for preparing ginseng fermentation liquor through fermentation of plant lactobacillus HCS03-001. The method provided by the invention comprises the following steps: combining a ginseng enzymolysis extracting solution and a second extracting solution, or combining a first extracting solution and the second extracting solution of ginseng, adding isomaltooligosacharide, and sterilizing to obtain a fermentation substrate; inoculating a bacterium solution of the plant lactobacillus HCS03-001 into the culture medium for activation culture to obtain an activated bacterium solution; inoculating the activated bacterial liquid into a fermentation substrate according to the inoculation amount of 4-6% v / v, centrifuging after fermentation, and sterilizing supernate to obtain the ginseng fermentation liquid. The ginseng fermentation liquor prepared by the method provided by the invention is rich in Rg3 and other ginseng rare saponins, the problems of insufficient dissolution of active ingredients and extremely low content of the rare saponins in traditional extraction are solved, and in addition, dry ingredients (Rg1 and Re) which can cause dryness-heat feeling, parched mouth and scorched tongue, annoyance and other symptoms of people in the ginseng can be reduced.

Even among low-alcohol beverages, there is a demand for beer-flavored drinks that possess the sweetness and crisp aftertaste suitable for beer-flavored beverages. [Solution] A beer-flavored beverage having an alcohol content of 6.0 (v / v)% or less, a ratio of alcohol content (unit: (v / v)%) to isomaltose (A) content (unit: g / 100mL) [alcohol content / (A)] of 45.0 or less, and a total content of monosaccharides or disaccharides consisting of glucose (C1), fructose (C2), sucrose (D1), and maltose (D2) of 0.10 g / 100mL or less.

The present invention provides a lyophilized composition containing tolvaptan sodium phosphate and a preparation method for the lyophilized composition. One or more of maltitol, raffinose, dextran, stachyose, arabinogalactan, fructooligosaccharide, galactooligosaccharide, isomaltooligosaccharide, polyethylene glycol, xylitol, or sorbitol is / are used as a lyoprotectant, so that the problem in the prior art that lyophilized compositions containing tolvaptan sodium phosphate are difficult to satisfy both high stability and high clarity; provided a tolvaptan sodium phosphate-containing composition which satisfies both high stability and high clarity, has a high dissolution rate and is easy to apply, reducing generation of impurities; in addition, in the preparation and long-term storage process, better stability is achieved, the opalescence and turbidity of liquid medicine during reconstitution and redissolution are avoided, dissolution is easier, and safe application of the product is facilitated.

The present invention relates to the field of food and drug processing technology, and in particular to a lutein or its derivative particles for tablet preparations, and a preparation method and application thereof. The lutein or its derivative particles of the present invention are prepared from raw materials comprising the following components in parts by weight: 60-80 parts of lutein and / or lutein derivatives, 3-6 parts of antioxidants, and 20-30 parts of auxiliary materials; wherein the auxiliary materials include corn starch, maltodextrin, oligomeric isomaltosaccharides, microcrystalline cellulose, and magnesium stearate. The granular preparation can better protect lutein or its derivatives, has high stability, and thus significantly improves the storage stability of lutein or its derivatives in products prepared using the granular preparations as raw materials; moreover, compared with embedded preparations, the granular preparations have a significantly higher loading capacity. In addition, the granular preparation preparation process is simple and easy, the conditions are mild, and no high-temperature treatment is required, and it has good prospects for industrial application.

The present invention relates to the use of monoester glycolipids or mixtures of monoester glycolipids in laundry detergent compositions. The monoester glycolipids or mixtures of monoester glycolipids include a carbohydrate fraction selected from the group consisting of maltose, cellobiose, trehalose, isomaltulose, lactulose, isomaltose and mixtures thereof.

The invention discloses sugar-free high-functionality isomaltooligosacharide and a preparation method thereof, and belongs to the technical field of functional sugar manufacturing. The invention discloses a preparation method of sugar-free high-functionality isomaltooligosacharide, which comprises the following steps of: converting starch into coarse ultrahigh maltose syrup by using high-temperature-resistant alpha-amylase, barley-beta-amylase and pullulanase, further refining the coarse ultrahigh maltose syrup through deslagging, decolorizing and ion exchange, adding glucosylase, and carrying out high-temperature hydrolysis to obtain the sugar-free high-functionality isomaltooligosacharide. And then purifying the crude isomaltooligosacharide by adopting a nanofiltration membrane, and removing glucose in the sugar liquid. The content of glucose and maltose in the prepared isomaltose hypgather is lower than 0.5%, the isomaltose hypgather meets the requirements of the current market for healthy and sugar-free high-functional products, the content of effective sugar IG < 2 + > P + IG3 is larger than or equal to 90%, and the product has higher functional components which are two times that of the current market products.

The invention discloses a fiber-containing pre-digested full-nutrient formula food and a preparation method thereof, and relates to the technical field of full-nutrient formula foods. The preparation method of the fiber-containing pre-digested full-nutrient formula food is simple to operate, the nutrient loss in the production process is less, the nutrient index control is accurate, and the fiber-containing pre-digested full-nutrient formula food is suitable for large-scale industrial production; the components are comprehensive and rich and are easy to digest and absorb by a body, soluble dietary fibers such as fructo-oligosaccharide, isomaltooligosacharide and xylooligosaccharide are especially added, and the intestinal burden of a patient is reduced to the maximum extent on the basis of ensuring reasonable diet of the patient through the synergistic effect of all the components, so that the health-care food has a good health-care effect. The rehabilitation of diseases of patients with gastrointestinal function insufficiency or partial deficiency states such as acute and chronic pancreatitis, diarrhea and inflammatory intestinal diseases can be promoted.

This invention relates to the field of biotechnology, and more particularly to a nicotinamide microcapsule, its preparation method, and its uses. The preparation method of the nicotinamide microcapsule includes the following steps: mixing gelatin and water and then heating the mixture to obtain a gelatin solution; under constant temperature conditions of 38-45℃, sequentially dissolving L-α-glycine phosphate, sodium alginate, isomaltooligosaccharide, nicotinamide, and vitamin C in the gelatin solution to obtain a mixed solution; adjusting the pH of the mixed solution and then continuously stirring to obtain the nicotinamide microcapsule. This nicotinamide microcapsule exhibits good encapsulation effect and high encapsulation rate, demonstrating excellent encapsulation ability for nicotinamide, effectively improving the absorption efficiency of nicotinamide, and enabling nicotinamide to be converted into more NAD+. + This leads to the production of more ATP, thereby increasing the bioavailability of nicotinamide.

The invention provides a composition for reducing the age of the intestinal tract, an oral composition formed by the composition and application, the composition for reducing the age of the intestinal tract comprises probiotics and prebiotics, and the probiotics are bifidobacteria; the prebiotics comprise two or more than two of polydextrose, isomaltooligosacharide and galactooligosaccharide. The composition can effectively influence and change the composition of intestinal bacteria of a body, promote the abundance of core characteristic bacteria representing young intestinal tracts to be increased and inhibit the growth of bacteria representing aging intestinal tracts, and people tests prove that the composition can effectively change the intestinal flora structure tending to be aged, reduce the age of the intestinal tracts and improve the immunity of the intestinal tracts. The intestinal tract becomes young.

The invention relates to an oligosaccharide debranching enzyme mutant with weakened product inhibition and application of the oligosaccharide debranching enzyme mutant, and belongs to the technical field of enzyme engineering. The 311th lysine and the 405th glutamic acid of the oligosaccharide debranching enzyme with the amino acid sequence as shown in SEQ ID NO.1 are found to be located at key positions of a substrate binding pocket through molecular docking, so that double mutants and three mutants are constructed, and the enzyme activity of the double mutants and the enzyme activity of the three mutants are respectively expressed and verified in escherichia coli; the three mutants V219A / K311M / E405A with the most obvious product inhibition effect weakening are obtained through screening, and the three mutants V219A / K311M / E405A are suitable for efficiently hydrolyzing various oligosaccharides such as maltose, isomaltose and panose to generate glucose. When glucose is produced by using an enzymic method, the oligosaccharide debranching enzyme mutant provided by the invention can further hydrolyze oligosaccharide in a starch saccharification liquid into glucose, so that the yield of glucose is improved, and the mutant has industrial production potential.

This invention provides a fecal microbial freeze-drying protective solution. Through the synergistic effect of components such as phosphorylcholine polymer, glycerol, trehalose, ergothioneine, L-cysteine, melibiose, isomaltooligosaccharide, and stachydrine, it effectively reduces the damage to bacterial activity during the freeze-drying process, improves the stability of freeze-drying preservation, and enhances the ability to maintain bacterial metabolic activity. It plays a significant role in maintaining the diversity and functional activity of the bacterial community.

The invention belongs to the field of liver cirrhosis and hepatocellular carcinoma marker detection, and particularly relates to application of a metabolic marker detection reagent in preparation of liver disease diagnosis products. The metabolic marker is a combination of Nicotinamide N-oxide, Pregnandiol 3-O-glucose, Guanidoacetic acid, Glycine, Isomaltose, Picolinic acid, 2-Hydroxycyanic acid, Quinolinic acid, Carnosine, 3-Methyluridine, 3-Methyluridine and Formiminoglumic acid, and the metabolic marker is a combination of the Nicotinamide N-oxide, the Pregnandiol 3-O-glucose acid, the Guanidoacetic acid, the Glycine, the Isomaltose, the Picolinic acid, the 2-Hydroxycyanic acid, the Quinolinic acid, the Carnosine, the 3-Methyluridine, the 3-Methyluridine and the Formiminoglumic acid. The liver disease is liver cirrhosis or hepatocellular carcinoma. The 12 urine metabolism markers are combined to accurately distinguish normal people from patients with liver cirrhosis, and normal people from patients with hepatocellular carcinoma. The kit provides a brand-new and objective biological basis for early diagnosis, treatment and prognosis evaluation of liver cirrhosis and hepatocellular carcinoma.