The present invention relates to a natural plant spice mixture with antibacterial function. It is characterized by that its raw material composition includes 25 ingredients of fresh garlic, fresh small garlic, onion, chives, pulstane, tangerine peel, pepper, coriander seed, leaf mustard seed, cinnamon bark, tsaoko fruit, dahurian angelica root, dried ginger, licorice, clove, crataegus fruit and others. Its preparation method includes the following steps; pulverizing, drying, sieving, impregnating with alcohol, preparing acid liquor, mixing, sterilizing and packaging.