131 results about "Flounder" patented technology

A fish allogenous frozen-sperm induced gynogenesis method comprises a frozen storage method of the allogenous fish sperm, a genetic inactivation method, an induced method for the gynogenesis diploid fry and an identification method for the gynogenesis fry. The present invention establishes the method adopting the frozen-sperm of the sea bass to induce the egg gynogenesis of a plurality of sea fishes for the first time, which selects the cold-shock starting time, cold-shock temperature and the cold-shock durance time that are separately suitable for fish eggs of the strip-spotted flounder, the turbot and the canine-tooth paralichthys olivaceus to establish a micro-satellite marking identification method of the gynogenesis fry. The percentage rate adopting the present invention to acquire the gynogenesis fry of the strip-spotted flounder, the turbot and the canine-tooth paralichthys olivaceus is 7.8 percentages to 40.7 percentages, 26 percentages to 34 percentages and 10 percentages to 25 percentages respectively. The established method of the present invention is characterized in advancing, high efficiency, accuracy and reliability, which has an important application value in fish gender controlling and the production of the all-female fries and has a broad popularization and application prospect on fish cultivation and fish breeding.

The invention discloses a flounder-type underwater glider. An electronic cabin comprises a pressure-resisting cabin, a gravity and gravity center adjustment mechanism, a control system and a lithium battery unit. The inside of the pressure-resist cabin is partitioned into a central area, a middle circular area and an outer circular area. The lithium battery unit is arranged in the middle circular area. The control system includes an overwater control system and an underwater control system which are connected in a wireless manner. The underwater control system and the electronic part of surveying equipment are arranged in the middle circular area uniformly. The gravity and gravity center adjustment mechanism comprises four water bag pump units in same structure, and the four water bag pump units are distributed in the outer circular area symmetrically. The flounder-type underwater glider has the advantages of low cost, low noise, high endurance and high flexibility; the gravity and posture of the glider can be adjusted to realize zigzag or spiral trajectory through controlling the four water bag pump units distributed symmetrically. The flounder-type underwater glider can carry a plurality of underwater scientific-investigation and survey devices simultaneously, continuous data collection in extensive sea areas can be completed automatically, and the data can be transmitted back in real time.

The invention relates to a method for the fish genealogy establishment and the disease resistance and high yield improved type selection. By adopting the technologies, such as group selection, genealogy selection, hybridization and artificial infection, as well as the sieving of the quick growth genealogy and disease resistance genealogy, the genealogy which has the advantages of strong flounder disease resistance and quick growth is selected, and the selection method for the disease resistance and high yield high quality improved type fish is established. The technology has the advantages of strong pertinence, high selection efficiency, obvious effect, etc. and the survival percent of the disease resistance flounder fish fry is more than 80 percent, which is increased by 30 percent more than the survival percent of the common flounder fish fry; the growth speed of the disease resistance flounder fish fry is also increased by more than 10 percent. The method in the invention opens up a new technical approach for culturing the disease resistance fish variety. As a disease resistance high yielding variety selection method applied to the improved fish type selection, the method is suitable for the popularization and the application on all the cultured fishes, and has important significance and application value in culturing the cultured fish of disease resistance and high yield improved type.

The invention relates to a water treatment technique, in particular to a method for biological resource recovery of industrial marine fish aquaculture wastewater. In the method, macroscopic algae, filter feeders, sediment animals and carnivorous fishes are cultured in the industrial marine fish aquaculture wastewater of which the water temperature is between 4 and 27 DEG C to form a biological filter to treat the wastewater discharged by industrial fish aquaculture, wherein the macroscopic algae are warm water macroscopic algae and cold water macroscopic algae; the filter feeders are filtering seashells and fishes, the seashells comprise one or more of oyster, sea-mussel and scallop, and the filtering fishes are barracudas; the carnivorous fishes comprise one or more of weever, flounder and black marine rock fish; and the sediment animals are Stichopus japonicus Selenka. In the method, based on the ecology principle, the macroscopic algae, the filter feeders, the sediment animals and the carnivorous fishes are combined together, and the macroscopic algae, the filter feeders, the sediment animals and the carnivorous fishes coordinate with one another and serve as the biological filter to comprehensively treat the wastewater discharged by the industrial fish aquaculture.

The invention relates to a detection method of a bastard halibut rhabdovirus in the aquatic livestock pathogen detection and diagnosis technical field. A real-time quantitative reverse transcription-polymerase chain reaction technology is adopted to detect the bastard halibut rhabdovirus of fish. A specific primer sequence and a fluorescence probe sequence are designed firstly and then nucleic acid of a sample to be detected is extracted; secondly, the real-time quantum reverse transcription-polymerase chain reaction is carried out, and ribonucleic acid containing target amplified fragments by in vitro transcription is used as a standard substance to draft a standard curve; finally, whether the sample to be detected contains the bastard halibut rhabdovirus is judged according to circulating valve value of the sample to be detected calculated by an apparatus software. The method of the invention can be widely applied to epidemic disease monitoring over aquatic plants of bastard halibut, weever, turbot, snapper sea bream, etc. and detection of the bastard halibut bullet-shaped virus in import and export trade. Compared with the prior art, the method of the invention is characterized by rapid detection, high efficiency, accurate quantum, strong specificity, wide detection scope, high sensitivity, etc.

The invention relates to a selection and culture method of excellent breed large-tooth flounder featuring fast growth and high survival rate. The technical method comprises: culture and reproduction regulation and control of large-tooth flounder breeding group, establishment of family by sperm freezing technology, culture and standardization of the family, determination and analysis of number and properties, estimation of genetic parameters, screening of excellent family, and establishment of excellent breed by inducing thelykaryon of large-tooth flounder to develop by using heterologous frozen sperms. The invention can select and culture new breed of cultured large-tooth flounder featuring fast growth and high survival rate, screen out four excellent large-tooth flounder families featuring fast growth and high survival rate (0703, 0719, 0750 and 0751), and establish seven excellent breeds of large-tooth flounder developed from thelykaryon (0920, 0921, 0927, 0938, 0939, 0946 and 0947). The technical method can be applied to new variety culture of large-tooth flounder cultivation, solve the problems of culturing large-tooth flounder fish in sea water such as slow growth and low survival rate, and enhances economic benefit of large-tooth flounder cultivation.

The invention discloses a compound immunopotentiator for left-eye and right-eye flounders, which contains burdock oligosaccharide, chitosan, chlorogenic acid, vitamin C, vitamin E and sodium alginate, wherein every 100 parts of the compound immunopotentiator contains the following components in parts by weight: 25 to 35 parts of the burdock oligosaccharide, 20 to 30 parts of the chitosan, 15 to 25 parts of the chlorogenic acid, 5 to 15 parts of the vitamin C, 5 to 15 parts of the vitamin E and 2 to 8 parts of the sodium alginate. The compound immunopotentiator for left-eye and right-eye flounders has the advantages of good performance and only 0.1 to 1.0% of the addition amount, can prominently enhance the immunity and the disease resistance of the flounders and raise the survival rate and the growth rate, has higher immune effect than other immunopotentiators, overcomes the immunosuppression phenomenon represented after long-term use of a single product and can be used in a full period; in addition, the compound immunopotentiator for left-eye and right-eye flounders overcomes the harm to cultivated aquatic products and human body caused by chemical disinfectants and antibiotics in prior disease control techniques, and settles the problems of strong specificity and high cost in prior aquatic vaccines.

The invention discloses a method for fluorescent double-labeling microscopic observation of marine flounder oosperm microtubules and cell nucleuses. The method comprises the following steps of carrying out fixing of an oosperm with a membrane, stripping a blastoderm of the fixed oosperm from a whole embryo by a dissecting needle, punching the stripped blastoderm, reducing or eliminating the influence produced by uncombined aldehyde groups on an experimental result by sodium borohydride, carrying out sample sealing and fluorescent double-labeling dyeing, and carrying out sample observation by a fluorescence microscope or a laser scanning confocal microscope. The method has low sample requirements, has simple processes, is convenient for observation, produces a clear result, has a high three-dimensional feel, can be used for dynamic change research on flounder oosperm microtubules and cell nucleuses from blastoderm formation respectively to 2-cell, 4-cell and 8-cell development stages, is suitable for other larger telolecithal eggs of marine flounder oosperms, and has a wide application range.

A flounder and flatfish engineering pond recirculating aquaculture system belongs to the technical field of marine aquaculture. The system comprises a water storage pond, a back water treatment pond, a coarse filtration pond, a conjoined combination aquaculture pond, a water feeding and drainage system, an oxygen supply device and a water quality monitoring system. On the one hand, the system still uses a traditional earthen pond aquaculture mode for culturing flounder and flatfish to grow in an environment similar to the wild so as to improve the quality of farmed flounder and flatfish. Meanwhile, engineering and systematic arrangement and layout are performed on the outdoor culture earthen pond to achieve factory and information management of flounder and flatfish pond culture, the output of farmed flounder and flatfish such as bastard halibut can reach to 1000-2000kg/mu (one mu equals about 1.667square meters), increasing 5-10 times of the output of traditional large single pond culture, and the culture efficiency and economic and ecological benefits are greatly improved.

An improved flounder gig comprising, in combination, a handle, a spear carried at one end of the handle having a pair of outwardly biased legs at the distal ends of which are diverging sharp prongs, and an elongated guide plate having a pair of spaced apart apertures slidably receiving said legs. The guide plate is moveable from a retracted position nearer the lower end of the handle in which the legs and prongs are allowed to assume an outwardly diverging relationship under the influence of the biased legs and a terminal position nearer the prongs in which the prongs are maintained in a closer relationship.

The invention relates to the field of microbial disease control and in particular relates to a probiotic with bacteriostatic ability. The probiotic is Enterobacter ludwigii-PC88, is collected in China General Microbiological Culture Collection Center (CGMCC) on June 24, 2013, and has a collection number of CGMCC No.7814. The probiotic is separated from intestinal tracts of healthy flounders. The pure culture of the probiotic has relatively strong inhibitory effects on aquatic pathogens such as vibrio splendidus, vibrio harveyi, vibrio parahaemolyticus, pseudomonas aeruginosa and vibrio anguillarum, also can inhibit growth of known human pathogens such as tritirachium album, staphylococcus aureus and klebsiella pneumoniae, and has resistance to ampicillin. The pure culture or composition of the probiotic can be used for control of aquaculture diseases and development of novel antibacterial materials, and has good application prospects.

The invention discloses a recombinant large-tooth flounder interleukin-6 protein and a preparation method thereof. The preparation method comprises the following steps: according to a DNA sequence of the large-tooth flounder interleukin-6 protein of paralichthys olivaceus, designing a primer and extracting the total RNA of the kidney fresh by a TRIzol Reagent method, carrying out RT-PCR augmentation, connecting a PCR product and a Pmd19T vector to form a recombinant plasmid, connecting with an expression vector Pet-32a(+) by double digestion, obtaining the recombinant plasmid and transforming the recombinant plasmid into escherichia coli BL21 (DE3); and realizing the soluble expression of the recombinant protein. As the expression process does not generate an inclusion body, the fusion protein for expressing the plasmid has histidine tags, thus being convenient to identification and purification of samples, avoiding the denaturation and renaturation of the samples, having simple operation, reducing the manufacturing cost, and more importantly, having no damage to a protein structure; and the invention has good purity of recombinant protein, high yield and difficult degradation, is applicable to industrial production and lays the foundation of studying the large-tooth flounder interleukin-6 protein of the paralichthys olivaceus.

A method for industrial raising pygmy flounder by natural spawning is characterized by that the seawater is filled in a spawning pool until water depth is 2-4 m, a light shielding cloth with 95% of optical shielding rate is installed above the spawning pool, the male and female pygmy flounder in the ratio of 2:1 are raised in the pool at 11-16 deg.C of water temp, and the spawning pool is artificially irradiated by 600-1500 lx for 12-16 hr perday.

The invention discloses a binding device of a small animal. The binding device comprises a binding device, a head sleeve and a tail sleeve, wherein the binding device is barrel-shaped, one end of the binding device is closed and the other end of the binding device is provided with an opening; the head sleeve is sheathed with one closed end of the binding device and is provided with a first connecting device; the tail cover is sheathed with one end, with the opening, of the binding device and is provided with a second connecting device matched with the first connecting device, and the bottom of the tail sleeve covering the opening is provided with a through hole through which the tail of the small animal passes. The binding device disclosed by the invention has the advantages that the rear legs can be prevented from pedaling out of the binding device when the experiment animal flounders, and when the binding device is matched with a non-invasive blood pressure monitoring system for use, the purpose of binding the experimental animal is effectively achieved, so that the benefit to shortening the monitoring time and improving the monitoring accuracy is achieved.

The invention aims at providing a transgenic method of a marine flounder. The transgenic method of the marine flounder comprises the following steps of: transferring plasmids with exogenous genes into pubescent testis of a milter; and collecting seminal fluid of the transgenic milter and fertilizing with mature eggs of a hen, so as to transfer the exogenous gene into the mature eggs through sperms. The method avoids the problem that lots of embryos are dead due to micro-injection to the oosperm. By adopting the method, the exogenous gene can be effectively transferred into the oosperm of the flounder; the integration of the exogenous gene integrated in a transgenic fry is 12 to 21%; a novel method is provided for the study on gene transfer of the flounder; the problem that the transgenosis is hardly carried out on the oosperm of the flounder by micro-injection is solved; and novel technical means are provided for breeding in the flounder genetic engineering.

A method of rearing aquatic species is provided, comprising feeding the aquatic species during at least part of the larval and/or post-larval stage with prey organisms, such as Aremia or rotifers, having in their total lipid content a DHA content of at least 12 wt %. The method is suitable for the rearing of fish species such as halibut, turbot, bass, bream and flounder.

A cross breeding method of fine variety of lefteye flounder is a cross breeding method for establishing high-yield cross breeding variety of lefteye flounder with the technologies of artificial selection, cross breeding, growth performance evaluation and anti-infective ability measurement. The method comprises the following methods: sieving of anti-infective cross parent, sieving of quickly-grown cross parent, cross breeding of lefteye flounders came from different countries, measurement of growth performance and anti-infective ability of cross seedlings. The method has the characteristics of strong pertinence, high efficiency, obvious effect, easy operation, and the like; the growth rate of cross breeding lefteye flounder seedlings is increased by about 30% compared with normal lefteye flounder seedlings, and the survival rate is increased by above 30% compared with the normal lefteye flounder seedlings. The technology develops a new way for curlturing seawater fish high-yield anti-infective variety, and is a high-yield fine variety culture method capable of being used in fish culture of fine variety; and the method is suitable for popularization and application of cultured fishes, and has important meanings and application values for improving culture yield of fishes.

The invention discloses a comprehensive stress-resistant flounder screening method. The method comprises culture system preparation and comprises the steps of detecting a culture environment, and checking whether or not the environment accords with a pre-set environment index; after the environment is checked to be qualified, placing juvenile fishes and adult fishes for breeding into the culture system; utilizing a fishing net capturing method to regularly screen the juvenile fishes for culture; utilizing a fishing net capturing and limiting method to screen the adult fishes for breeding for the first time; then conducting further feeding behavior screening; selecting fishes with a high condition factor in screened lively and healthy parent fishes as parent fishes for breeding. According to the comprehensive stress-resistant flounder screening method, by utilizing the stress physiological property of flounders for capturing, fishing net limiting and bait throwing and the theory that appetite recovery is closely related to disease infection stress after stress and by adopting the method of repeated screening and weeding out weak individuals, stress-resistant flounder individuals with strong appetite are screened from common flounder fry and the parent fishes for breeding group, the flounder fry and the parent fishes for breeding group keep healthy, and the culture yield and thesurvival rate of offspring are increased.

A method for culturing the lefteye flounder without environmental pollution includes such steps as filling fresh water in pool, immersing for a certain time, disinfecting, flushing several times, filtering, depositing and disinfecting seawater, filling it in the pool, culturing the fry of bastard halibut in the pool, feeding the granular bait, and regularly exchanging water and applying medicine.

The invention discloses a perilla frutescens kernel sauce and a preparation method thereof. The perilla frutescens seed sauce comprises the following stir-fried raw materials in parts by mass: 40 to 70 parts of perilla frutescens kernels, 5 to 15 parts of soybeans, 5 to 15 parts of dried flounder fish, 5 to 15 parts of scallop and 10 to 20 parts of perila seeds. According to the invention, the perilla frutescens kernel sauce is prepared by using the perilla frutescens kernels as the main raw material, using the soybeans as the auxiliary material and then matching with the dried flounder fish and the scallop to carry out seasoning, wherein perilla frutescens has higher linolenic acid content, the soybeans have higher protein content, and other nutritional ingredients of the perilla frutescens are not damaged in the stir-frying process, so that the prepared perilla frutescens sauce is a seasoning with high linolenic acid content and high protein content, and has rich and balanced nutrition and low price.

The invention relates to a preparation method for flounder herring products and belongs to the technical field of processing of aquatic products. The preparation method comprises the following steps: handling the raw materials; washing; cutting; curing; brushing; drying; packaging, wherein the step of curing indicates performing hybrid curing of dry curing and wet curing under the conditions of temperature at 8-12 DEG C and salinity being 10%; the step of drying indicates drying in the combined manner of drying with hot air at 40-60 DEG C in the initial stage and drying with cold air at 20 DEG C in the later stage. According to the preparation method provided by the invention, the flounder mesh is prepared into the herring products, the flavor and variety of the products are increased and the value-added processing is performed.

The invention discloses a method for breeding Edwardsiella tarda-resistant excellent flounder breed. The method mainly comprises the following steps: screening Edwardsiella tarda-resistant pedigrees specific microsatellite markers by an Edwardsiella tarda-infected flounder fry and disease resistance measuring method, an Edwardsiella tarda-resistant group screening method, an Edwardsiella tarda-resistant pedigrees screening method, an Edwardsiella tarda-resistant pedigrees specific microsatellite marker screening method and the like; establishing a disease-resistant pedigree offspring disease resistance and breeding survival rate measuring method, and establishing the Edwardsiella tarda-resistant excellent flounder breed. The flounder breed bred by the method has the characteristics of strong Edwardsiella tarda resistance, high breeding survival rate, low death rate and relatively high growth speed; the problem of massive death caused by an ascites disease due to the infection of Edwardsiella tarda in summer is basically solved; the method is suitable for being popularized and applied in flounder breeding farms in coastal areas in China.

The invention belongs to the technical field of marine organisms and particularly relates to a method for preserving large volume of lefteye flounder sperms by refrigeration. The method comprises the following processes: refrigerating large volume of lefteye flounder sperms; preserving the lefteye flounder sperms by liquid nitrogen; and unfreezing and eluting the preserved lefteye flounder sperms to obtain the sperms which have fertilization ability. The method comprises the following steps of: collecting lefteye flounder semen; placing the semen and anti-frozen liquid to a 5ml of freezing tube in a volume ratio of 1:1; pre-cooling the semen and the anti-frozen liquid at 0 to 4 DEG C for 9 to 11 minutes; lowering the temperature to -180 DEG C in section by section and placing the lefteye flounder semen in the liquid nitrogen for storing; and directly placing the lefteye flounder semen to water at a temperature of between 40 and 42 DEG C to melt the lefteye flounder semen and unfreezing the molten lefteye flounder semen at room temperature so as to obtain sperms with the fertilization ability. The method for preserving large volume of lefteye flounder sperms by refrigeration plays an important role in breeding, germplasm conservation, genetic diversity, sustainable aquaculture and the like for of rare seawater fishes such as lefteye flounder, and solves the problems of low preservation volume, low density, small preservation quantity and the like in prior art.

The invention relates to a nonspecific immunity compound preparation for indoor breeding flounder, and a use method thereof. The preparation is prepared by the following powdery effective components by weight: 0.25-0.35 kg of beta-glucan, 0.6-0.7 kg of rheum officinale, 0.23-0.33 kg of radix scutellariae, 0.42-0.52 kg of cortex phellodendri, 0.4-0.51 kg of andrographis paniculata, 0.45-0.55 kg of vitamin C phosphate and 0.1-0.2 kg of vitamin B. A use method of the preparation comprises the following steps of (1) adding the compound preparation to raw materials of flounder baits with balanced nutrients according to a proportion of 1:1,000, mixing uniformly and making the mixture into a particle bait by using a conventional preparation method for the particle baits; and (2) feeding the flounder with the compound preparation bait for a cycle (7 days) every other 10 or 14 days in a feeding production process, daily feeding amount being identical with a normal bait, until the flounder is bred into commercial fish and taken out from a pool. Particularly, in prevalence of diseases, the compound preparation bait is fed to the flounder every day. The preparation is scientific in compatibility, convenient for use and simple in operation, can obviously enhance nonspecific immunity and anti-stress capacity of the flounder, and further enhance disease resistance of the flounder body as well as improve a breeding effect.

The invention relates to an industrial culture device capable of simulating a flounder paralichthys wild benthic environment. The industrial culture device comprises a culture pond, wherein a general blow-off hole is formed in the middle of the bottom of the culture pond; a hollow water level column for adjusting a water level is mounted on the general blow-off hole; a horizontal porous carrying platform for segmenting the bottom of the culture pond to form a lower blow-off layer space is arranged above the bottom of the culture pond; a sand filtering thin film covers on the upper surface of the porous carrying platform; a natural sea sand layer is arranged on the sand filtering thin film; an upper air guide pipe, which is connected with an air pump, is mounted on a peripheral side wall of the culture pond on the upper surface of the natural sea sand layer; and airflow blown out from an air guide hole of the upper air guide pipe passes through the upper surface of the natural sea sand layer, so that the natural sea sand layer is stirred. By adopting the industrial culture device provided by the invention, separation of blow-off and a habitat environment is realized and the quality of the habitat environment on an upper layer of a pond bottom is guaranteed, so that the condition of blind side melanism of flounder paralichthys is greatly reduced.