Method for breeding Edwardsiella tarda-resistant excellent flounder breed

Abstract

The invention discloses a method for breeding Edwardsiella tarda-resistant excellent flounder breed. The method mainly comprises the following steps: screening Edwardsiella tarda-resistant pedigrees specific microsatellite markers by an Edwardsiella tarda-infected flounder fry and disease resistance measuring method, an Edwardsiella tarda-resistant group screening method, an Edwardsiella tarda-resistant pedigrees screening method, an Edwardsiella tarda-resistant pedigrees specific microsatellite marker screening method and the like; establishing a disease-resistant pedigree offspring disease resistance and breeding survival rate measuring method, and establishing the Edwardsiella tarda-resistant excellent flounder breed. The flounder breed bred by the method has the characteristics of strong Edwardsiella tarda resistance, high breeding survival rate, low death rate and relatively high growth speed; the problem of massive death caused by an ascites disease due to the infection of Edwardsiella tarda in summer is basically solved; the method is suitable for being popularized and applied in flounder breeding farms in coastal areas in China.

Description

technical field [0001] The invention belongs to the technical field of fish genetics and breeding in the aquaculture industry, and in particular relates to a breeding method for an excellent strain of flounder with resistance to tardy Edwardsiella. Background technique [0002] Flounder is an important marine fish cultured in China, Japan and Korea, and it is also a high-protein aquatic product that people like. At present, the aquaculture output of flounder in my country is about 20,000 tons per year, and the output value is 2.5-3 billion yuan. In addition to meeting the needs of the domestic market, flounder is also one of the main aquatic products that are exported to earn foreign exchange. However, with the rapid development of the flounder aquaculture industry, some problems have emerged one after another, especially the frequent occurrence of diseases, germplasm degradation, and low survival rate of seedlings, which cause economic losses of nearly 1 billion yuan to th...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to establish a method for breeding excellent strains of flounder resistant to tardy Edwardsiella, to make up for the deficiencies in the prior art, to provide a technical method for the cultivation of fine varieties of fish resistant to tardy Edwardsiella, and to solve the problem of flounder aquaculture. The problem of the lack of breeding methods for excellent strains resistant to tardy Edwardsiella in China, and to provide technical means for the breeding of excellent disease-resistant strains for the fish farming industry

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