183 results about "Marine fish" patented technology

The present disclosure relates to the technical field of marine fish cultivation and breeding, and in particular to an industrialized cultivation method for a cephalopholissonnerati fry. The method includes: a step of sequentially performing food acclimation and nutrient enrichment on a cephalopholissonnerati parent fish under conditions of 25-29° C. of water temperature and running water, to obtain the parent fish with mature gonad development, and other steps.

The present disclosure relates to the technical field of marine fish cultivation and breeding, and in particular to an industrialized cultivation method for a Cephalopholissonnerati fry. The method includes: a step of sequentially performing food acclimation and nutrient enrichment on a Cephalopholissonnerati parent fish under conditions of 25-29° C. of water temperature and running water, to obtain the parent fish with mature gonad development, and other steps.

The invention belongs to the technical field of collagen peptide extraction, and discloses a preparation process of low-fishy-smell marine fish skin collagen peptide, which is used for solving the problems of incomplete fishy smell removal and high protein loss rate in the traditional fishy smell removal process. Comprising the following steps: (1) immersing fish skin into water, adding lipase, cleaning the fish skin with pure water, and homogenizing to obtain homogenate; (2) adding saccharomyces cerevisiae and purple perilla juice into the homogenate, carrying out heat preservation and enzymolysis at 30 DEG C, then heating to 45 DEG C, adding compound enzyme for enzymolysis, and heating for enzyme deactivation to obtain collagen peptide enzymatic hydrolysate; (3) adding activated carbon and diatomite into the collagen peptide enzymatic hydrolysate, keeping the temperature at 70 DEG C, stirring, and carrying out filter pressing and refined filtration to obtain a collagen peptide decoloring solution; (4) sterilizing and concentrating the collagen peptide decoloring solution to obtain a collagen peptide concentrated solution; and (5) drying the collagen peptide concentrated solution to obtain collagen peptide powder. The collagen peptide prepared by the method is almost free of fishy smell and low in protein loss.

The invention belongs to the technical field of biology, and particularly discloses a high-temperature-resistant antibacterial protein Cystatin B sourced from marine fish as well as a preparation method and application of the high-temperature-resistant antibacterial protein Cystatin B. The high-temperature-resistant antibacterial protein Cystatin B is derived from pomfret, and the amino acid sequence of the high-temperature-resistant antibacterial protein Cystatin B is as shown in SEQ ID NO. 1; the nucleotide sequence of the gene for coding the high-temperature-resistant antibacterial protein Cystatin B is as shown in SEQ ID NO.2, and the sequences of primers adopted for cloning the gene are as shown in SEQ ID NO.3-4. The high-temperature-resistant antibacterial protein Cystatin B has a remarkable antibacterial effect on gram-negative bacteria such as escherichia coli, vibrio parahaemolyticus and edwardsiella tarda as well as gram-positive bacteria such as staphylococcus aureus; and after being treated at the temperature of 100 DEG C, the antibacterial agent still can keep relatively high antibacterial activity. The antibacterial protein is simple in preparation method, can replace traditional antibiotics, and can quickly and efficiently prevent and treat various common bacterial diseases in aquaculture.

The invention discloses a fishery breeding training platform for marine robot breeding and stability training, and relates to the field of aquaculture equipment, the fishery breeding training platform comprises a ship body, the ship body is provided with a first breeding bin and a second breeding bin which are used for breeding marine fishes, the first breeding bin is used for feeding of workers, and the second breeding bin is used for feeding of the workers. The second breeding bin is used for feeding of the intelligent robot, a feed bin and a medicine bin are arranged on the ship body, a storage plate is arranged on the ship body, a plurality of first driving parts used for supporting the storage plate are arranged on the ship body, the first driving parts are used for controlling the storage plate to shake, and an action collecting chamber is arranged on the ship body. A plurality of action acquisition monitors connected with the action acquisition chamber are arranged on the ship body, and the action acquisition chamber analyzes and controls the intelligent robot to work according to the work of workers. Data are provided for the intelligent robot to provide the mariculture platform, the training convenience of the intelligent robot is improved, and the training stability of the intelligent robot on the mariculture platform is ensured.

The invention relates to marine fish breeding, and particularly discloses an indoor full-artificial breeding method for black epinephelus, which specifically comprises the key technical links of nutrient enrichment of parent fish, hormone induced spawning, artificial insemination and fertilized egg incubation, indoor breeding of fry, compound feed domestication and the like. By controlling water temperature, illumination, salinity and nutritional conditions, synchronous maturation and efficient ovulation of parent gonads of the epinephelus nigatosus are achieved, the fertilization rate reaches 95% or above, and the hatching rate exceeds 85%. According to the method, the whole-process artificial controllable breeding of the epinephelus nigatosus from the parent fish to the fry is realized, a new variety is provided for the epinephelus culture industry, and a feasible technical approach is also provided for the artificial breeding and improved variety breeding of the wild epinephelus nigatosus in the island reef of the South China Sea.

The invention provides a combined lactobacillus salivarius YS-UR90 and a preparation method of the combined lactobacillus salivarius YS-UR90. The saliva combined lactobacillus provided by the invention can secrete protease and glycosidase, and the HCT and FLS gene expression levels of the saliva combined lactobacillus are high, so that the saliva combined lactobacillus can promote phenolic acid active ingredients to generate biotransformation during fermentation, and the proportion of chlorogenic acid and the like in fermentation liquor is increased. For example, marine fish oligopeptide and casein phosphopeptides are used as a composite nitrogen source, dandelion powder is used as a functional substrate, and a composite fermentation product obtained through fermentation of saliva and lactobacillus has the effect of promoting uric acid excretion and has a certain protection effect on kidney injury. The compound fermentation product has the effect of improving hyperuricemia mainly by inhibiting XOD activity, increasing serum urea nitrogen level, improving defect expression of uric acid synthesis gene HPRT1, inhibiting expression of uric acid reabsorption URAT1 and GLUT9 genes and improving expression of excretion genes OAT1 and ABCG2.

The invention discloses a method for separating, purifying and transplanting spermatogonial stem cells of lateolabrax japonicus, the spermatogonial stem cells of lateolabrax japonicus are separated and purified, marine fish larvae or freshwater fish larvae are used as receptor larvae, and then the spermatogonial stem cells of lateolabrax japonicus are transplanted to abdominal cavities of the receptor larvae. The spermatogonial stem cells obtained through separation are complete in number, structure and function and low in cell clustering rate. After the transplanted spermatogonial stem cells are subjected to fluorescence labeling, the migration and proliferation processes of donor germ cells can be observed in receptor fishes of marine fish fries or freshwater fish fries, so that the receptor fishes with the chimeric germline are obtained. According to the method, through xenotransplantation of the spermatogonial stem cells, a'belly borrowing reproduction 'breeding mode is provided for the marine fishes, a new way for gamete preservation and rapid propagation is provided for the marine fishes with the long reproductive cycle, and a new method and a new thought are provided for genetic improvement and germplasm resource preservation.

The invention belongs to the technical field of microorganisms and biological medicines, and particularly relates to marine-source lactobacillus plantarum HYC-1 with cholesterol lowering and alpha-glucosidase inhibiting activity and application of the marine-source lactobacillus plantarum HYC-1. The novel lactobacillus plantarum HYC-1 is screened from intestinal tracts and contents of marine fishes, the strain has efficient cholesterol lowering activity and alpha-glucosidase inhibition activity at the same time, and experimental results show that the in-vitro cholesterol lowering rate of lactobacillus plantarum HYC-1 fermentation liquor can reach 89.4%, the alpha-glucosidase inhibition rate can reach 93.5%, and the lactobacillus plantarum HYC-1 fermentation liquor can be used for preparing the cholesterol lowering agent. One bacterium has double effects and unique functions; besides, the strain shows excellent tolerance and high safety, so that the strain can be widely applied to food, health care products and medicines, especially to preparation and research and development of medicines with the efficacy of regulating blood fat and blood sugar.

The invention discloses crushing and mixing integrated equipment for marine fish feed processing and a processing method, and relates to the technical field of marine fish feed processing.The crushing and mixing integrated equipment comprises a processing bin, a plurality of crushing bins are embedded in the processing bin, crushing mechanisms are arranged in the crushing bins, sieve plates are arranged in the processing bin, and crushing mechanisms are arranged in the processing bin; a base is arranged on the lower portion in the processing bin, a movable bin is movably installed on the inner wall of the processing bin in an embedded mode, a driving motor is installed at the top of the processing bin, a driving rod is installed at the output end of the driving motor, and a stirring mechanism and a cooling mechanism are arranged below the side wall of the driving rod. And the stirring mechanism comprises two lower stirring rods and two upper stirring rods. The contact area of the lower stirring rod and the upper stirring rod with raw materials is increased, so that the stirring effect is improved, the temperature of the raw materials can be maintained during mixing, and the situation that internal components of the raw materials are inactivated due to temperature rise is avoided.

The invention discloses a marine fish uric acid lowering peptide with kidney protection activity as well as a preparation method and application of the marine fish uric acid lowering peptide. The marine fish uric acid reducing peptide Trp-Gly-Val-Pro-Glu, WGVPE for short, with kidney protection activity is prepared, and the marine fish uric acid reducing peptide Trp-Gly-Val-Pro-Glu, WGVPE for short, can remarkably reduce the blood uric acid level in a body, promote uric acid excretion and improve pathological damage of the kidney. In addition, the pentapeptide provided by the invention can be used in common food, food additives and functional food for preventing and treating hyperuricemia and kidney injury or health care products or medicines for relieving hyperuricemia and kidney injury, and has a good application prospect.

The invention discloses a preparation method of composite active peptide with anti-wrinkle, whitening and freckle-removing effects from marine fish byproducts, cod byproducts, namely fish heads, fish bones and fish skins, are used as raw materials and are mixed according to the mass ratio of 3: 3: 4, and the composite active peptide is prepared through the steps of pretreatment, enzymolysis, enzyme deactivation, centrifugal separation and purification. A compound enzyme used in the enzymolysis step is composed of trypsin and papain according to a certain proportion, and the enzyme addition amount, the enzymolysis time, the enzymolysis temperature and other conditions are optimized. The prepared composite active peptide with the anti-wrinkle, whitening and freckle-removing effects from the marine fish by-product has good anti-wrinkle, whitening and freckle-removing effects, resource utilization of the cod by-product is achieved, and the composite active peptide has high application value.

The invention discloses a primer group, a kit and a method for rapidly and visually detecting silver salmon components, belongs to the technical field of nucleic acid detection of marine fish products, and particularly relates to a method for rapidly and visually detecting the silver salmon components by using an LAMP (loop-mediated isothermal amplification) technology. The invention provides a primer combination suitable for rapid nucleic acid detection and a corresponding kit, the primer combination comprises an outer primer pair and an inner primer pair, and the kit integrates the primer combination and an optimized LAMP reaction mixed solution. The detection means has a plurality of prominent characteristics that during detection, only DNA of a sample to be detected needs to be added into an LAMP reaction mixed solution prepared in advance, and the detection work can be carried out by means of common constant-temperature equipment. After the whole detection process is about 60 minutes, the result interpretation can be completed by observing the color development condition with naked eyes, and when the reaction mixed solution is yellow, the reaction mixed solution is judged to be positive; and if the color is red, determining that the color is negative. Due to the design of closed tube detection, the hidden danger of aerosol pollution caused by uncovering operation is effectively avoided. The method is excellent in specificity and relatively high in detection sensitivity, and can detect 10 <-1 > ng of genome DNA or a sample with the mass fraction of 1% at least. The method has the advantages of good result reproducibility, simplicity and convenience in operation and low cost. The method is particularly suitable for being applied to scenes with relatively limited resources such as basic units and field detection, the cost investment in the aspect of supervision can be remarkably reduced, and the efficiency of detection work is improved.

The invention provides a full-indoor artificial breeding method for plectropomus leopardus, and belongs to the technical field of artificial breeding of marine fishes. The full-indoor artificial breeding method for the plectropomus leopardus comprises the steps of earlier-stage incubation of fertilized eggs, incubation in an indoor breeding pond, breeding in an indoor flowing water pond, breeding in an indoor circulating water pond and the like. By regulating and controlling illumination, the irritability of the fries is greatly reduced, the ecological conditions of the fries are effectively optimized, and the health condition and adaptability of the fries are improved. Meanwhile, different types and different total amounts of biological bait combinations are fed in the larva stage, the juvenile stage and the fish stage of the plectropomus leopardus, transition is made in each bait transfer period, enteritis caused by excessive intake of larvae and juveniles and too little intake caused by inadaptation of bait transfer are effectively reduced, and the problem that the survival rate is low in the bait transfer period can be effectively solved.

The invention discloses a primer group, a kit and a method for rapidly identifying components of salmon red, belongs to the technical field of nucleic acid detection of marine fish meat products, and particularly relates to a method for rapidly identifying the components of salmon red by using an LAMP (loop-mediated isothermal amplification) technology. The invention provides a primer group for rapid nucleic acid detection and a matched kit, the primer group is composed of an outer primer pair, an inner primer pair and a single loop primer, and the kit comprises the primer group and an optimized LAMP reaction mixed solution. The detection method has the following remarkable characteristics: the DNA of a sample to be detected only needs to be added into the pre-prepared LAMP reaction mixed solution, the detection can be completed by using common constant-temperature equipment, the whole process is about 60 minutes, the color development result can be observed by naked eyes to realize interpretation, if the reaction mixed solution is yellow, the reaction mixed solution is positive, and if the reaction mixed solution is red, the reaction mixed solution is negative. According to the closed tube detection design, the aerosol pollution risk caused by uncovering operation is avoided. The method is excellent in specificity, detection sensitivity (the lowest 10 <-1 > ng genome DNA can be detected or the mass fraction is 1%) and result reproducibility, has the characteristics of simplicity and convenience in operation and low cost, is particularly suitable for resource limited scenes such as grassroots units and field detection, and can remarkably reduce the supervision cost and improve the detection efficiency.

The invention provides a primer combination for a multiple detection kit for marine pathogenic bacteria and the multiple detection kit. The primer combination comprises primer pairs and probes which are used for detecting 13 marine pathogenic bacteria: vibrio alginolyticus, vibrio parahaemolyticus, vibrio cholerae, vibrio vulnificus, vibrio marine fish, vibrio river, vibrio menivibrio, vibrio mimicus, vibrio harveyi, vibrio occultus, vibrio horii, plesiomonas and aeromonas, and the sequence of the primer combination is as shown in SEQ1-SEQ39. According to the present invention, the genomic DNA of 13 marine pathogenic bacteria is identified and detected in one digital PCR reaction through the ultra-multiple digital PCR fluorescence coding technology, and the kit not only covers the common vibrio species such as vibrio cholerae, vibrio vulnificus, vibrio parahaemolyticus and the like, but also can identify the sea area vibrio, vibrio holii, vibrio occultus and the like, and the kit has good sensitivity, specificity and accuracy.

The invention provides an intelligent marine fish culture device and method based on vision and vortex, and relates to the technical field of aquaculture. According to the device, a layered monitoring module is used for collecting environment data, fish body state data and residual bait data, the environment data comprise water temperature data and dissolved oxygen data, and the fish body state data comprise fish body behavior data, barrel bottom capsule distribution data and fish body surface data; the edge calculation unit is used for performing instruction calculation according to threshold values corresponding to the environment data, the fish body state data and the residual bait data to obtain an eddy current regulation and control instruction and a feeding instruction; the intelligent vortex module is used for switching corresponding working modes according to the vortex regulation and control instruction, the working modes comprise a normal breeding mode and a capsule removing mode, and the intelligent feeder is used for feeding the breeding area according to the feeding instruction. The problem of low marine fish disease monitoring and protection efficiency in the prior art is solved.

The invention discloses a fish and shellfish symbiosis seaborne net cage culture system and method, and relates to the technical field of aquaculture, and the method comprises the following steps: arranging a culture net cage in an offshore sea area; fish fries are put into a surrounding net, bivalve shellfishes are put into a shellfish culture piece cage, and the shellfish culture piece cage is hung in the culture net cage; feeding bait irregularly, checking the integrity of the shellfish culture cage and the covering condition of attachments regularly, and making a treatment means according to the covering rate; in the later period of culture, the adult fishes with the body length exceeding a preset value are caught irregularly in a fishing mode, and after the culture period is finished, the remaining adult fishes are caught in a concentrated mode by increasing the height of the purse seine; the shellfish culture piece cage is lifted out of the water surface to collect adult shellfish. The culture system disclosed by the invention comprises a culture net cage and shellfish culture piece cages, wherein the shellfish culture piece cages are hung in a purse seine. According to the method, mutualism of marine fishes and bivalve shellfish can be achieved, the resource utilization rate and the culture benefits are improved, and diversified fishery development can be achieved.

The invention discloses skipjack glycopeptide with anti-inflammatory activity and a preparation method and application thereof, and belongs to the technical field of deep processing of marine fish food. The technical problem to be solved is to improve the anti-inflammatory effect and the cell proliferation effect. The key point of the technical scheme is as follows: the molecular weight distribution of the skipjack glycopeptide with the anti-inflammatory activity comprises the following components in percentage by mass: 10.8-13.2% of components with the molecular weight smaller than or equal to 300 Da, and 86.8-89.2% of components with the molecular weight ranging from 300 Da to 2000 Da.

The utility model relates to a marine fish large-scale fry breeding incubation net cage, which comprises at least one incubation net cage, each incubation net cage comprises an outer cage body, an isolation flexible film is arranged at the position 1 / 3 away from the bottom in the outer cage body, a net bag is arranged in the outer cage body above the isolation flexible film, a through rotating main shaft is arranged in the outer cage body below the isolation flexible film, and the rotating main shaft is connected with the isolation flexible film. Fan blades are fixed on the rotating main shaft at intervals, the fan blades are oval, the rotating main shaft is located at the non-midpoint position of the long axis of the fan blades, and every two adjacent fan blades are symmetrically distributed with the rotating main shaft as the axis; one end of the rotating main shaft is connected with a transmission assembly; and the other end of the rotating main shaft extends into the adjacent hatching net cage. The fan blades which rotate along with the rotating main shaft to alternately jack up different positions of the bottom of the net bag are arranged at the bottom of the hatching net cage, so that roes deposited at the bottom of the net bag are turned over, oxygen deficit of the roes is avoided, the number of the roes placed in a single net cage can be properly increased, and the hatching scale is increased.

1. Name of the designed product: packing jar (sea fish paste). 2. Use of the designed product: for product packing. 3. Design points of the designed product: combination of shape and pattern. 4. Picture or photo showing the design points: perspective view.

The present invention relates to the technical field of marine fishing LED lights, and discloses a mackerel LED light for marine fishing, including a copper-shaped plastic shell of the LED light. A lens is integrally formed and connected to the bottom of the copper-shaped plastic shell of the LED light, and a rare gas inner cavity is formed between the copper-shaped plastic shell of the LED light and the lens. The copper-shaped plastic shell of the LED light has a convex-shaped structure, and metal flow blocks are fixedly connected to the top of the copper-shaped plastic shell of the LED light at equal intervals in the circumferential direction. Air guide grooves are opened at equal intervals in the circumferential direction at the bottom of the copper-shaped plastic shell of the LED light, and the air guide grooves penetrate through to the top of the copper-shaped plastic shell of the LED light. By integrally forming and connecting a lens to the bottom of the copper-shaped plastic shell of the LED light, and forming a rare gas inner cavity between the copper-shaped plastic shell of the LED light and the lens, users can, by professional means, fill different rare gas mixtures into the rare gas inner cavity. When the power is turned on, colorful lamp colors can be obtained, thereby improving the trapping ability for fish schools.

The invention provides a marine ranch, and belongs to the technical field of marine fish grazing, and the marine ranch comprises a shell, a connecting shell is mounted on the shell, a stand column is in threaded connection with the connecting shell, a net plate is fixedly connected to the side surface of the stand column, a connecting ring is fixedly connected to the stand column, a connecting plate is mounted on the connecting ring, and a first floating block and a pressing block are slidably mounted on the stand column; a plate spring is arranged between every two adjacent connecting plates, a connecting belt is fixedly connected to the first floating block, the connecting belt is guided by a guide block to be connected with a pressing block, the guide block is fixedly installed on the stand column, an adjusting assembly is arranged on the side face of the shell, a baffle is installed on the adjusting assembly, and a butt joint assembly is arranged between the shell and the connecting shell. A connecting frame is installed on the butt joint assembly, and a third connecting plate is slidably installed in the connecting frame. The problems that an existing marine ranch cannot conduct closed centralized treatment on fish excrement, and cannot automatically press an assembly structure through buoyancy are solved.

The application discloses a kind of mild extraction observation counting device and method of marine fish fry, and device includes sequentially connected suction line, observation platform, weak fry sorting device and overflow drain pipe.Suction line is equipped with variable frequency negative pressure pump and transparent steel wire hose;Observation platform is equipped with multistage slow-flow buffer structure inside, and observation area and shooting fixed mechanism are equipped in side and top;Weak fry sorting device is equipped with cyclone flow guide structure inside, and form rotating flow field, so that weak fry is detained in central slow-flow area and is discharged through bottom discharge port, and healthy fry is discharged from top with outside water flow;Overflow drain pipe inlet is bent upwards, and automatic overflow drainage is realized.The application integrates fry mild suction, dynamic counting, cyclone weak fry elimination, automatic drainage and raw water replacement, has no exposed metal, high waterproof level, adapts to seawater environment, significantly reduces fry damage rate and labor cost, and is especially suitable for transportation and screening of marine fish fry such as Epinephelus, Pseudosciaena crocea and the like.

A formula, APSW (Anti Pathogen Seawater), for preparing seawater for fish farms and aquariums that contains some or all the normal ingredients of sea salt with the exception of having low or no sulfates. Testing by the applicant has revealed that sea water without sulfates eliminates amyloodiniosis (Amyloodinium ocellatum), one of the most serious diseases of warm water marine fish.

This invention relates to the field of aquatic compound feed technology, specifically to a special feed for mullet, silver carp, and golden carp cultured in saline-alkali land in Northwest China. The feed is characterized by being composed of the following raw materials by weight percentage: 10%–14% alfalfa meal, 16%–22% soybean meal, 16%–22% corn meal, 5%–7% molasses, 1%–3% yeast powder, 2%–5% lactic acid bacteria preparation, 2%–5% premixed feed for aquatic life in saline-alkali land in Northwest China, 1%–4% fish oil, and 1%–5% vegetable oil. This invention solves the problems of low survival rate, high feed conversion ratio, easy antagonistic inactivation of probiotics, fish oil oxidation, poor water resistance of pellets, and insufficient regional adaptability of traditional feeds. Compared with commercially available ordinary marine fish feeds, the survival rate of juvenile fish is increased by 15% to 30%, the feed conversion ratio is reduced by 10% to 20%, the Vibrio inhibition rate is ≥80%, the power consumption per ton of feed is reduced by 25% to 35%, and the raw material cost is reduced by 30% to 45%. It is suitable for large-scale aquaculture in saline-alkali land in Northwest China and has important value for improving the quality and efficiency of fisheries, ecological restoration, and rural revitalization.

The invention provides a compound feed additive for relieving stress response of marine fishes, which is prepared by the following steps: mixing raw materials such as hermetia illucens protein and the like to prepare a premix, and embedding the premix by using a lac-glycyrrhizic acid system to prepare lac microcapsules; then modifying the fucoidin by adopting glycyrrhizin and gamma-linolenic acid, embedding rhodosporidium palustris and bacillus pumilus by using the fucoidin to prepare compound bacterium nano-particles, and finally mixing the lac microcapsules and the compound bacterium nano-particles in proportion. According to the invention, excessive secretion of cortisol of marine fish can be effectively inhibited, hermetia illucens protein provides rich nutrition and reduces muscle decomposition, and compound microorganisms are beneficial to maintaining intestinal microecological balance and improving the utilization rate of nutrition. The prepared compound feed additive can be directly splashed into a culture or transportation water body or added into a feed, so that the stress reaction of the marine fish is effectively relieved, the survival rate of the marine fish is increased, and powerful support is provided for culture of the marine fish.

The invention relates to the technical field of food processing, and particularly discloses a processing method for preparing sea cucumber viscera into powder. The raw materials of the product comprise fresh sea cucumber viscera and composite auxiliary materials, wherein the composite auxiliary materials comprise marine fish collagen peptide and euphausia superba powder; the preparation method comprises the following steps: pretreating fresh sea cucumbers, taking out viscera, cleaning, mixing the viscera with composite auxiliary materials, pulping, filtering to obtain composite juice, carrying out ultrasonic-assisted enzymatic degradation on the composite juice, inactivating bio-enzyme, carrying out low-temperature concentration and drying to obtain primary powder, carrying out non-thermal sterilization and high-pressure sterilization on the primary powder, and packaging to obtain the sea cucumber beverage. And cooling and filling in a sterile environment. The product can be used as a functional food raw material, and has the advantages of reservation of heat-sensitive active ingredients such as sea cucumber saponin and stable quality; in addition, the preparation method disclosed by the invention has the advantages of efficiently utilizing byproducts of the internal organs of the sea cucumbers, being mild in process, not destroying active ingredients and controllable in operation, and resource waste and active ingredient loss can be reduced.

The invention belongs to the technical field of parasite sampling, particularly relates to an imported marine fish pathogenic parasite sampling and detecting device, and provides the following scheme aiming at the problems of low manual sampling efficiency, inaccurate positioning and easy sample damage in existing marine fish parasite quarantine. Comprising a sampling base, a movable supporting plate, a sampling area identification assembly, a sampling pipe and a negative pressure separation assembly. The sampling area identification assembly can losslessly and accurately position a suspected parasite infection area in a fish body. The sampling pipe is provided with an annular gas guide cavity and an annular liquid guide cavity, sterile gas and normal saline can be sprayed cooperatively, and minimally invasive and efficient stripping and recycling of insect bodies are achieved. The negative pressure separation assembly adopts a double-chamber design, a micro-filtration membrane is arranged in the negative pressure separation assembly, a sample can be shunted and independently treated in single operation, and a negative pressure liquid extraction pipe of the negative pressure separation assembly has an automatic valve function of negative pressure ventilation and normal pressure closing, so that subsequent in-situ fixation is facilitated. According to the invention, the whole process automation from precise minimally invasive sampling to automatic separation and fixation is realized.

The invention discloses a primer group, a kit and a method for rapidly identifying a pink salmon component, belongs to the technical field of nucleic acid detection of marine fish meat products, and particularly relates to a method for rapidly identifying the pink salmon component by using an LAMP (Loop-Mediated Isothermal Amplification) technology. The primer group comprises a pair of outer primers, a pair of inner primers and a loop primer, and the kit comprises the primer group and LAMP amplification reaction liquid. According to the present invention, the sample DNA is only required to be added into the LAMP amplification reaction liquid, the visual qualitative detection can be achieved through the simple constant temperature device, the operation is simple, the reaction time is only 1 h, the cover does not need to be opened after the reaction is completed, the experiment result can be obtained by observing the color change of the reaction liquid, the high concentration DNA aerosol pollution is effectively prevented, and the detection result is accurate. When the color of the reaction liquid is yellow, the detected sample is determined to be positive, and when the color of the reaction liquid is red, the detected sample is determined to be negative. The method also has the advantages of strong specificity, high sensitivity, good repeatability and low cost, the genome DNA sensitivity can reach 1 ng, the mass fraction sensitivity can reach 1%, the supervision cost is greatly saved, the supervision efficiency is improved, and the method is suitable for rapid detection of vast basic levels and field assurance with capital shortage.