84results about How to "Reduce metabolic burden" patented technology

The invention belongs to the field of beverage preparation, and particularly to a brown lactobacillus beverage and a preparation method of the brown lactobacillus beverage. The brown lactobacillus beverage is prepared from skim milk powder, white granulated sugar, glucose, high fructose corn syrup, a sweetening agent, a stabilizer, probiotics strains and pure water. The preparation method comprises steps of yeast milk burdening, hydration, homogenization, browning, sterilization, inoculation, fermentation, base materials burdening, base materials sterilizing, milk mixing, homogenization, sterile filling, and refrigeration. The product provided by the invention has sucrose content 30-100% lower than that of a general product, and zero fat content. The product can really relieve the metabolic burden of a human body and is healthy nutrient. In addition, the problem of poor product stability caused by low sugar is solved, and the content of activated probiotics during the fermentation of probiotics strains is greater than or equal to 3*10<8>cfu/mL, so the product has a high nutritive value.

The invention discloses a dealcoholic sobering-up pharmaceutical preparation and a preparing method thereof; the pharmaceutical preparation is prepared by poria, hawthorn, pueraria, ganoderma lucidum, and hoveniae semoveniae semen. Compared with the prior art, the pharmaceutical preparation has very significant dealcoholic sobering-up effect, protects gastric mucosa, does not hurt the gaster, adjusts gastric motility in two ways, inhibits vomiting, nausea and abdominal distention, directly decomposes alcohol and improves capacity for liquor, resists hypnosis and alleviates headache and head halo, speeds up the emptying time of alcohol in the intestine and gaster, reduces the metabolism burden of the liver and kidney, accelerates the alcohol metabolism in the blood, protects the liver, enhances the immunity and relieves the fatigue. The pharmaceutical preparation is prepared by pure natural plants, and has less medicinal odor, easy quality control, and excellent effects for protecting liver, nourishing gaster and strengthening spleen.

The invention belongs to the field of biological medicine and specifically discloses a nucleic acid-drug conjugate based on phosphorothioate modified nucleic acid, a drug delivery system and a preparation method thereof. Phosphorothioate groups in the phosphorothioate modified nucleic acid react with groups modified on drug molecules and capable of generating electrophilic reaction with the phosphorothioate groups, so as to form the nucleic acid-drug conjugate; the nucleic acid-drug conjugate can be self-assembled into drug-containing nano-carriers in various forms for drug delivery, in the manner of selecting different nucleotide sequences including functional nucleic acid; compared with the prior art, the invention has the advantages that the nucleic acid-drug conjugate can be acquired through a simple solid-phase synthesis technology, grafting sites and assembling forms of drug molecules on nucleic acid skeleton can be accurately controlled and the method has universality to chemotherapeutic drugs; according to the nucleic acid-drug conjugate, the drug delivery system, the preparation method and the application thereof disclosed by the invention, physicochemical properties and in vivo distribution properties of chemotherapeutic drugs are obviously improved, therapeutic effect thereof can be promoted and combination therapy of gene therapy and chemotherapy can be realized.

The invention provides derivatives of ticagerlor or pharmaceutically acceptable salts thereof, wherein R1, R2 and R3 in a formula representing the derivatives or the pharmaceutically acceptable salts thereof can be selected from amino acid residues or acetylsalicyloyl groups. The invention also provides a preparation method for the derivatives of ticagerlor or the pharmaceutically acceptable salts thereof and further provides application of the derivatives of ticagerlor or the pharmaceutically acceptable salts thereof in preparation of drugs used for treating blood coagulation-related diseases. The derivatives provided in the invention can improve bioavailability of ticagerlor or enhance the drug combination effect of ticagerlor and aspirin.

The invention relates to a butylphthalide- and edaravone-containing compound injection and a preparation method thereof. The injection of every 100 ml contains 25-50 mg of active ingredient butylphthalide, 12.5-50 mg of edaravone, as well as absolute ethyl alcohol, hydroxypropyl-beta-cyclodextrin, an osmotic pressure regulator, an antioxidant and the like. With the adoption of the butylphthalide- and edaravone-containing compound injection, the administration dosage is greatly lowered, the burden of liver metabolism is reduced, and the incidence rate of liver adverse reactions is lowered. According to determination, with the adoption of the butylphthalide- and edaravone-containing compound injection, the administration dosage of the edaravone can be reduced by at least 20%, besides, the compound injection has a better curative effect, and the incidence rate of the liver adverse reactions is also remarkably lowered.

The invention provides a preparation method of a chiral isoxadifen-ethyl compound. The compound prepared by the method is a single enantiomer. The preparation method is simple, few in byproduct, highin product yield and wide in application prospect. In addition, the invention further provides a herbicide safener. The herbicide safener contains the chiral isoxadifen-ethyl compound serving as an active ingredient, so that application range of herbicide can be expanded effectively.

The invention belongs to the technical field of bioengineering, and relates to breeding of industrial microorganisms, in particular to a chassis strain for producing alkaline protease as well as a construction method and application of the chassis strain. The invention provides a bacillus amyloliquefaciens gene engineering strain, which does not express six extracellular protease genes aprE, bpr, vpr, mpr, nprE and epr on a bacillus amyloliquefaciens genome, an extracellular polysaccharide gene cluster eps, a polyglutamic acid gene cluster pgs and a bacteriophage related gene with a nucleotide sequence as shown in SEQ ID NO: 1, and preferably, the bacillus amyloliquefaciens gene engineering strain also expresses an alkaline protease gene aprE in a heterologous overexpression manner. According to actual conditions in experiments and production, adverse factors influencing fermentation performance are analyzed, the chassis strain for producing alkaline protease at high yield is finally obtained through transformation and gene simplification of a genetic engineering host, and a new thought is provided for construction of the chassis strain for producing the alkaline protease at high yield.

The invention discloses new application of arginine, which is the application of arginine or a pharmaceutically acceptable salt, an ester or a mixture of the salt and the ester in preparing topical pharmaceutic preparations with at least one of the following effects: (1) treating and/or facilitating wound healing, and (2) reducing scar formation. Results in pharmacodynamics experiments show that the external use of L-arginine can remarkably facilitate trauma healing of rat skin as well as reduce the area of a wound scar. Arginine not only can be used for treatment and healing of traumas but also can be used for other skin diseases, including a pressure ulcer (bedsore), skin injuries caused by dermatitis, scald, burn and the like.

The invention relates to the technical field of food processing, in particular to high-fiber calcium reinforced collagen jelly and a preparation method thereof. The jelly comprises the following components of 1%-10% of sea fish collagen oligopeptide powder, 8%-12% of resistant dextrin, 0.5%-2.0% of casein phosphopeptides, 0.15%-0.35% of calcium citrate, 1%-5% of fruit powder or fruit juice rich invitamin C, 1%-5% of sugar alcohol, 0.1%-2.0% of a thickening agent, 0.01%-0.045% of a sweetening agent, 0.05%-2.0% of a sour agent, 0.01%-0.05% of potassium sorbate and the balance of water. The prepared jelly can effectively strengthen the effect of enriching the calcium, is good in properties and stability, sour, sweet and smooth in mouth feel, and easy to digest and absorb, does not enable weight to be increased, does not corrode teeth, can be eaten by diabetics, is convenient to carry, green and healthy, and is free from any pigment and essence for coloring and aroma increasing.

An invertose powder injection is prepared from glucose and pectose through preparing aseptic pectose, preparing aseptic glucose, sterilizing tubular bottle, sterilizing butyl rubber plug, mixing aseptic pectose with aseptic glucose to become invertose, loading in aseptic tubular bottle, sealing, and packing.

The present invention provides a method for transferring and expressing heterologous DNA in a non-plant host cell. The vector used in this method includes a backbone having a first origin of replication capable of maintaining heterologous DNA as a single copy in an Escherichia coli host cell. The vector further includes a unique restriction endonuclease cleavage site for insertion of heterologous DNA, and left and right Agrobacterium T-DNA border sequences flanking the unique restriction endonuclease cleavage site. In certain host cells, the T-DNA border sequences allow introduction of heterologous DNA located between the left and right T-DNA border sequences into a host cell. In preferred embodiments, the vector includes a second origin of replication capable of maintaining heterologous DNA as a single copy in a host cell such as Agrobacterium species or other prokaryotic cells.

The invention provides a recombinant bacillus subtilis, construction method and use thereof, wherein the cell's own FMMs are used as a space scaffold, and a multi-enzyme complex is constructed from specific marker proteins FloA and FloT, such that an artificial substrate channel is formed, and the cell metabolic burden is effectively reduced. The recombinant Bacillus subtilis of the invention can efficiently synthesize GlcNAc without affecting cell life activity, and can also limit the toxic intermediate metabolite GlcN-6-P near the plasma membrane to reduce or eliminate its inhibition on cell activity. In the process of shaking flask fermentation using complex medium, the yield of acetyl glucosamine of the control strain BSG-C was only 0.45 g.L⁻¹, while that of BSG-AT, BSG-ATP, BSG-ATPB increased to 5.29 g.L⁻¹, 6.22 g.L⁻¹ and 8.48 g.L⁻¹ respectively. The construction method of recombinant Bacillus subtilis is simple, easy to use and has a good application prospect.

The invention discloses a drug-coated balloon catheter comprises a pushing catheter with a certain axial length, at least one drug-coated balloon arranged at the distal end of the pushing catheter anda sheath movably sheathed outside the drug-coated balloon. The sheath can move axially along the push catheter and the drug coated balloon is received within the sheath prior to expansion. In the drug-coated balloon catheter of the present invention, the sheath has a protective effect on the drug coating on the surface of the drug-coated balloon during the transportation process, and the blood flow is prevented from scouring the drug coating. After reaching the lesion, the slidable sheath is withdrawn proximally. The drug-coated balloon protrudes from the sheath and is exposed to the vascularenvironment to be filled, thereby effectively reducing the rate of drug loss during delivery and improving the effectiveness and safety of the drug-coated balloon catheter.

The invention provides a composition with the effect of preventing and treating complications of a dialysis patient, and a preparation method and application of the composition. The composition is prepared from the components in parts by mass: 5-33 parts of whey proteins, 1-20 parts of casein or salts of the casein, 1-50 parts of maltodextrin, 1-10 parts of corn syrup and 5-75 parts of sunflower seed oil. The blood potassium level can be controlled to be lower than 5.0 mmol/L, the blood phosphorus level is controlled to be lower than 1.78 mmol/L, the complications such as protein malnutrition,hyperphosphatemia, hyperkalemia and hypertension of the dialysis patient are comprehensively relieved, the dialysis interval time of the dialysis patient is prolonged, and the harm of dialysis to thebody is reduced. In addition, the composition in each unit dose is brewed with 60 mL of water, the liquid intake burden is relieved and is equivalent to the osmotic pressure of the human body, gastrointestinal tolerance is better, and patient compliance is higher.

The invention discloses medical formula nutritional meal replacement powder for controlling hyperglycemia and a preparation method thereof. Forty-five raw materials are divided into five categories, according to the characteristics of the five categories, different preparation methods are divided, then according to the performance of medicinal ingredients contained in the five categories of raw materials, the medicinal active ingredients are extracted with different methods, the health care functions of liver protection, kidney protection, blood enriching and the like are achieved while diabetes is treated, the viscera are conditioned, human body yin and yang are balanced, human body trace elements are supplemented, and diabetes is comprehensively conditioned.

Described herein are methods and compositions for producing a gene of interest (GOD, which, in certain embodiments, can reduce the metabolic burden on cells and reduce decoupling of GOI production from marker production, as compared to prior art methods. The methods relate to positive selection and negative selection approaches to establishing high GOI-producing cell lines, e.g., CHO lines. In certain embodiments, the methods comprise transfecting a cell with (a) an oligonucleotide comprising a GOI and a non-coding RNA, and (b) an oligonucleotide encoding a selection protein; wherein the non-coding RNA promotes or inhibits production of the selection protein. The cell producing the can be identified and/or selected as a result of or by detecting the absence or the presence of the selection protein.

The invention discloses a method for removing phthalate esters in water utilizing an UV (ultraviolet)/ozone/chlorella degradation composite process. The method comprises the following steps of: 1) firstly sampling polluted water containing phthalates, and carrying out UV/ozone combined process pre-test to determine the UV/ozone combined process parameters: UV intensity, ozone introduction amount and the treatment time T when the removal rate of the phthalates in water reaches 90%; 2) pre-treating the polluted water containing phthalates for 0.3-0.4 T by using the UV/ozone combined process under the conditions of the UV intensity and ozone introduction amount; and 3) then injecting the pre-treated water into a chlorella breeding tank and carrying out biodegradation treatment under the action of chlorella. In the method disclosed by the invention, the algae can adapt to the adverse reactions of PAEs (phthalic acid esters) in advance by the UV/ozone pre-treatment, and the metabolic burden in the initial stage of biodegradation treatment is reduced, thereby saving energy, shortening the treatment time and lowering the construction cost. The method is suitable for large-area water source pollution.

The invention relates to the field of foods, and particularly provides a nutrient health-care food as well as a preparation method and application thereof. The nutrient health-care food mainly comprises the following raw materials of ginseng, lotus seeds, coix seeds, fructus momordicae, thallus laminariae, black wood ears, carrots, dendrobium nobile, Chinese wolfberry fruits, Chinese yams, poria cocos, lily bulbs, black beans, green tea, oat, purslane herbs, rice beans, phaseolus coccineus and semen sterculiae lychnophorae. According to the nutrient health-care food, through a reasonable compounding ratio, various nutrient substances desired by human bodies are provided, so that after people insist on eating the nutrient health-care food, the improvement effect on symptoms of eccyclomastopathy and dysmenorrhea of women is obvious, and the immunity of human bodies is also enhanced to a certain extent. The preparation method of the nutrient health-care food is simple, and convenient andcontrollable in making technology, and the nutrient health-care food is convenient to eat and free from side effects.

The invention belongs to the technical field of fish culture, and particularly relates to bait capable of enhancing the disease resistance of juvenile carp. The bait is prepared from vinasse, barley,soybeans, pear residues, oyster mushroom, purple cabbage, asparagus, vitamin C, madasiatic acid, a feed additive and coconut oil. The bait is easy to digest and absorb, has high reducing capacity, free radical scavenging capacity and lipid peroxidation inhibiting capacity, and can effectively retard the cell senescence, prevent the formation of lipid peroxide, promote the healthy growth of the body, promote the proliferation of new cells, promote the growth, development and improvement of organs, reduce the metabolic burden of hepatopancreas, effectively improve the metabolic capacity of the body, improve the enzymatic activity of the body and improve the nutrient absorption, transportation and transformation capacity, so that the anti-virus and anti-germ capacity of the body is effectively enhanced, the disease rate and death rate of juvenile carp are significantly reduced, the fast and robust growth of juvenile carp is promoted, the culture cycle is shortened, the culture cost is reduced, and the economic benefit of farmers is increased.

The invention discloses a solid beverage suitable for sports acrobatics. The solid beverage comprises the following raw materials in parts by weight: 0.1-2 parts of anhydrous dextrose, 0.1-2 parts ofgalacto-oligosaccharide, 0.1-2 parts of whey protein powders, 0.1-2 parts of ocean fish oligopeptide powders, 0.1-2 parts of elastin peptide, 0.1-2 parts of Daucus carota L. var. sativa Hoffm powders,0.1-2 parts of resistant dextrin, 0.1-2 parts of milk minerals, 0.1-2 parts of calcium hydrophosphate, 0.1-2 parts of yeast vitamin D and a proper quantity of probiotics. The solid beverage disclosedby the invention can guarantee that the body quickly obtains energy, an abdominal distension feeling is unlikely to generate, a user can feel light during the sport acrobatics, meanwhile, the blood glucose does not rise too quickly, an athlete can be supplied with continuous energy during the sports acrobatics so as to be favorable for stabilizing the blood glucose and enhance a brain function, so that the athlete performs more stably in a sports acrobatics process, and meanwhile, the solid beverage can accelerate muscle to recover, improve muscle synthesis and quicken strength gaining, and has a positive influence on the explosive power during the sports acrobatics.

The invention discloses a medicinal preparation and a preparation method thereof. The medicinal preparation contains 5-methyl-2-phenyl-1,2-dihydropyrazol-3-one, 3-butylisobenzofuran-1(3H)-one and camphol, as well as pharmaceutically-acceptable auxiliary materials, and a preparation formulation is injection liquid or a freeze-dried agent. The injection liquid provided by the invention can obviouslyreduce the medicine administration dosage of 5-methyl-2-phenyl-1,2-dihydropyrazol-3-one; and besides, the 3-butylisobenzofuran-1(3H)-one is administrated in the form of injection, compared with an orally-taken manner, the form of injection can notably reduce the medicine administration dosage, so that liver metabolism burden can be reduced, and the medicinal preparation has the advantages of being low in medicine administration dosage, stable in properties, low in incidence rate of adverse reactions of the liver and the like.