550results about How to "High viable count" patented technology

The invention discloses bio-organic fertilizer, and belongs to the technical field of organic fertilizer. The proportion by weight of all raw materials of the bio-organic fertilizer is dried chicken manure : edible mushroom : compound microbial agent : corn flour or rice bran : functional strain = 110-140 : 390-360 : 0.1-0.5 ; 0.1. The compound microbial agent comprises mould, bacillus, lactic acid bacteria, actinomycetes and saccharomycetes. The strains are efficient viable bacteria. Each gram of the compound microbial agent comprises the number of the mould ranges from 5 hundred million to 8 hundred million, the number of the efficient viable bacteria of bacillus ranges from 12 hundred million to 15 hundred million, and the number of the actinomycetes ranges from 10 hundred million to 12 hundred million and the saccharomycetes of 15 hundred million to 18 million. According to the bio-organic fertilizer, the chicken manure and mushroom dregs serve as main materials, and the high-quality organic fertilizer can be fermented quickly by adding the compound microbial agent and the functional strain.

The invention provides a lactobacillus helveticus microcapsule as well as the preparation and application thereof, pertaining to the embedding technology of lactobacillus helveticus and aiming at microencapsulating the lactobacillus helveticus so as to solve the problems of low embedding efficiency and embedding yield in single-layer or double-layer embedment. The technical proposal adopted by the invention is that isolated soy protein, microporous starch and sodium alginate are respectively taken as the first, the second and the third layers of wall materials for microencapsulation; the three-layer embedment is carried out to the lactobacillus helveticus for the first time, wherein, in the process of preparing bacterial suspension, an orthogonal optimization protective agent is adopted to improve the livability after freeze drying. The microcapsule of the invention has strong heat resistance and acid resistance, solves the inactivation problem caused by gastric acid, digestive enzyme and antibiotic, etc. to active bacillus, is capable of releasing when reaching large intestine, and balances intestinal flora. The reusability of fermented milk is good. The lactobacillus helveticus used by the invention enriches the variety of probiotic bacteria microcapsule and is a good dietary supplement.

The invention discloses lactobacillus plantarum fermented fruit and vegetable juice blends and a preparation method thereof. The lactobacillus plantarum fermented fruit and vegetable juice blends comprises the following raw material components: 10-90 parts of fruit and vegetable original juice, 8 parts of white sugar, 0.1-0.5 part of thickening stabilizing agent and 0.01-0.03 part of compound antioxidant. The invention also provides a preparation method of the lactobacillus plantarum fermented fruit and vegetable juice blends. The lactobacillus plantarum fermented fruit and vegetable juice blends provided by the invention is reasonable in nutrient matching; through screening and fermenting optimal lactobacillus plantarum strains, the content of amino acid in the fruit and vegetable juice is increased, novel vitamins and organic acids are synthesized, aroma constituents are generated, the original bitter and astringent taste of vegetables is improved, nutrients of different fruits and vegetables can be included in one final product so that different nutrient demands of consumers are met; meanwhile, the problem of sediment of the product within a guarantee period is solved through the addition of the thickening stabilizing agent, and the problem of color uniformity of the product within the guarantee period is solved through the addition of the compound antioxidant.

The invention relates to a method for preparing active lactic acid bacteria fermented vegetable and fruit juice. First white grape juice, yeast powder and glucose consist of ferment matrix, then acid and alkaline modifier is added in the fermented matrix, and pH value is adjusted to 6.0-7.0, then fermented using lactic acid bacteria, then lactic acid bacteria ferment liquor is obtained, then adding the liquor in vegetable and fruit juice according to common practice, adjusting nutrient of vegetable and fruit juice and adjusting pH value optimal for lactic acid bacteria fermentation, making viable bacteria number amounting to more than 106cfu/mL. The viable bacteria or its secreted metabolite has an function for suppressing growth of putrefactive bacteria of intestinal tract, so can balance the bacteria colony of intestinal tract, enhance immunity and delay aging etc.

The invention discloses a chicken manure fermentation organic fertilizer, which is prepared from the following raw materials: chicken manure, straw powders, konjak flour, corn flour, cellulose, zeolite, humic acid, potassium dihydrogen phosphate, urea, peat soil, a leavening agent, a soil improvement agent, a film coating agent, potassium sulfate, borax and calcium superphosphate. The above raw materials are subjected to the integrated treatment of anaerobic fermentation and aerobic fermentation, so that the organic fertilizer is prepared through the fermentation of the chicken manure. The chicken manure fermentation organic fertilizer is complete in fermentation effect, and is capable of killing plant pathogenic bacteria, eggs, weed seeds and other harmful microorganisms in the chicken manure, and generating a large amount of nutrients beneficial to the plant growth and the plant absorption. The process is simple and easy to implement. The raw materials of the prepared organic fertilizer are rich in nutrition. Meanwhile, the prepared organic fertilizer is high in fertilizer efficiency and rich in nutrition, and can coordinate the soil nutrient balance, improve the soil nutrient, promote the plant growth, increase the plant yield and improve the plant quality.

The invention belongs to the field of beverage preparation, and particularly to a brown lactobacillus beverage and a preparation method of the brown lactobacillus beverage. The brown lactobacillus beverage is prepared from skim milk powder, white granulated sugar, glucose, high fructose corn syrup, a sweetening agent, a stabilizer, probiotics strains and pure water. The preparation method comprises steps of yeast milk burdening, hydration, homogenization, browning, sterilization, inoculation, fermentation, base materials burdening, base materials sterilizing, milk mixing, homogenization, sterile filling, and refrigeration. The product provided by the invention has sucrose content 30-100% lower than that of a general product, and zero fat content. The product can really relieve the metabolic burden of a human body and is healthy nutrient. In addition, the problem of poor product stability caused by low sugar is solved, and the content of activated probiotics during the fermentation of probiotics strains is greater than or equal to 3*10<8>cfu/mL, so the product has a high nutritive value.

The invention relates to the field of microbial control, in particular to bacillusamyloliquefaciens subsp Lh-1 and application thereof. With a preservation number of CGMCC No.8548, the bacillusamyloliquefaciens subsp Lh-1 is applied in control of Verticillium dahliae, erwinia carotovora, ralstonia solanacearum, valsa leucostoma, Valsa Mali Miyabe et Yamada, Phytophthora capsici and phytophthora infestans. According to the invention, the bacillusamyloliquefaciens subsp Lh-1 and pesticide and chitosan oligosaccharide with dual effects of fertilizer and pesticide are combined into an Lh-1 microecological agent, the viable count is small, the dosage is small, the production process is simple, the cost is low, and the Lh-1 microecological agent has the dual functions of disease prevention and growth promotion. Nursery test proves that reduce the disease incidence of crop is reduced, especially for control of Verticillium dahliae, the disease incidence drops to 6%, and the control effect is significant. In addition, the Lh-1 microecological agent is a biological strain preparation, is completely free of a series of problems caused by use of chemical pesticides, thus being conducive to pollution-free production of crops, and farmers can have no need for pesticide or reduce the dosage of chemical pesticide, thus greatly saving the expenditure.

The invention discloses a biological salt-degrading bacterial fertilizer consisting of the following components by weight proportion: 0.0013 to 0.0045 portions of gibberellin, 0.00002 to 0.0002 portions of cytokinins, 15 to 35 portions of bacillus megaterium, 50 to 80 portions of salt-degrading flora, 5 to 15 portions of amino acid and 2 to 10 portions of humic acid. The materials are evenly mixed and air dried. The bacterial fertilizer is formed by being extracted from marine microorganisms, rejuvenation, expanding propagation and being remixed with a plurality of trace elements. The bacterial fertilizer is scientific in formulation and very strong in activity, can be widely applied to salt lick improvement and a secondary salinization treatment, improves and optimizes soil environment and improves the deteriorated soil environment caused by the long-term use of chemical fertilizers and salt containing organic fertilizers (dungs). The bacterial fertilizer can facilitate the development of the roots of crops and can improve the ability of absorbing night soil. By applying the biological salt-degrading bacterial fertilizer, the fertilizer can facilitate the even seedling, uniform seedling and strong seedling at the earlier stage of crops, the strong nutrient transmission capacity at the middle stage and no premature senescence at the later stage.

The invention discloses a fermented product added with a lactobacillus plantarum proliferation agent. The lactobacillus plantarum proliferation agent degrades an enzymolysis mixture after plant protein reaction through protease or a protease-containing substance, and the mass percentage of the lactobacillus plantarum proliferation agent contained in the fermented product is 0.1-20%. By adding the lactobacillus plantarum proliferation agent, the fermented product can be fermented milk, viable bacterial drinking water or a viable bacterial cosmetic. The fermented product has the following advantages that the proliferation agent belongs to a natural edible mixture and does not produce side effects on a human body after being added to the fermented product; 2, by using the proliferation agent, the fermentation time of the fermented product can be greatly shortened, and pollution caused by harmful infectious microbes is avoided; 3, by adding the proliferation agent, the number of living bacteria in the fermented product can be up to 1.0 * 109 cfu/g, the number of the living bacteria is largely higher than those of living bacteria produced by other proliferation agents; 4, by using the proliferation agent, the cost of the fermented product having large living bacterial number is reduced.

The invention discloses a preparation method for a low-lactose fermented dairy product. The preparation method has two modes; in one of the modes, lactase is added into milk and ferment is inoculated at the same time, so that the low-lactose fermented dairy product is prepared; in the other mode, lactase is first added into fresh milk, so that lactose is hydrolyzed into glucose and galactose, and ferment is then inoculated, so that the fermented dairy product is prepared. The low-lactose fermented dairy product prepared by the preparation method not only has the characteristics of low lactose content and high viable count, but also has the specific flavor and taste of fermented milk, the change of acidity is little in the process of later storage, and the taste is good. The preparation method is applicable to the preparation of low-lactose fermented dairy products.

The invention relates to an Acinetobacter calcoaceticus WG-6 capable of degrading oil and a culture method and application thereof, and belongs to the technical field of microorganisms. The bacterial strain has been collected in a China General Microbiological Culture Collection Center on June 11th, 2010 with a collection number of CGMCC No.3915. The Acinetobacter calcoaceticus can directly decompose oil contaminants into a nutrient material for utilization and growth and reproduction, and has remarkable oil degradation effect. The invention also provides a high-density microbial inoculum prepared by mixing the Acinetobacter calcoaceticus and other components, the microbial inoculum can rapidly decompose the oil, and the other components in the microbial inoculum can improve the microecological environment of soil, and accelerate the soil mediation progress.

The invention discloses a lactic acid bacteria semi-solid fermentation product. The product is prepared by the following processes: inoculating lactic acid bacteria in raw materials and fermenting at a temperature of 24-44 DEG C for 24-40h to obtain the product. The raw materials comprise 4.0-5.0g of wheat bran, 1.2-1.8g of soybean meal, 0.8-1.2g of glucose or brown sugar, and water, wherein water is added until the total amount of the raw materials is up to 100mL. The inoculation amount of the lactic acid bacteria is 1.0-2.0*10<7>cfu/100mL, wherein the lactic acid bacteria is composed of Lactobacillus plantarum and Pediococcus pentosaceus with a strain viable count ratio of (3:10)-(10:3). The lactic acid bacteria semi-solid fermentation product for increasing growth performance of animals provided by the invention is the first fluid fermentation feed independently developed in China according to the national conditions for culture, inherits the advantages of the traditional solid fermentation feeds, and at the same time has the advantages of being more convenient, fresher, more significant in feeding effects, lower in cost and the like.

The invention discloses achromobacter xylosoxidans subsp.xylosoxidans LH-N25 with CGMCC No.6972. The invention also discloses a heterotrophic nitrification and aerobic denitrification microorganism bactericide. The microorganism bactericide comprises achromobacter xylosoxidans subsp.xylosoxidans LH-N25 or also comprises paracoccus aminovorans LH-N40; and the strains are combined according to any ratio. The invention also discloses a preparation method of the microorganism bactericide. The microorganism bactericide disclosed by the invention not only can solve the problem that total nitrogen cannot be easily removed in the traditional reactor, but also can effectively remove ammonia nitrogen and total nitrogen in a water body in the same reactor, and has tolerance and degradation ability on the toxic substances such as phenols, amines, hetercyclics, cyanogens and polyaromatic hydrocarbon in the wastewater at the same time. The microorganism bactericide is especially suitable for chemical wastewater containing nitrogen, is simple in treatment process, stable in effect, and resistant to impact of environmental toxic substances, and has a wide application prospect in treatment of chemical wastewater containing nitrogen.

The invention discloses a method for producing a microorganism feed additive by utilizing probiotics and mixed meal. The method comprises the following steps: adding water into fermentation substrates respectively, stirring materials, and then cooking and sterilizing; adding a mixed bacterial liquid into the sterilized fermentation substrates and stirring uniformly after the temperature is lowered to 28-35 DEG C, and carrying out aerobic fermentation under constant temperature; introducing a lactic acid bacterium after the fermentation substrates are fermented and mixed, and then carrying out secondary fermentation; and drying materials after fermentation at a lower temperature, and crushing so as to obtain a microorganism feed additive finished product. The fermentation substrates comprise germ meal, corn spray slurry fibers, rapeseed meal and rice bran meal; the rice mixed bacterial liquid is obtained through mixing bacterial liquids of candida utilis, bacillus licheniformis and cryytococcus neoformans; and the lactic acid bacterium is lactobacillus plantarum, and the inoculum size is 5-10% of the weight of the fermentation substrates.

The invention relates to a high-density culture method of bifidobacterium lactis V9 and a preparation method of a freeze-dried powder of the bifidobacterium lactis V9, and belongs to the field of probiotics fermentation. The bifidobacterium lactis V9 (Bifidobacterium animalis subsp.lactis V9) is acid-resistant and cholate-resistant probiotics which are separated from the intestinal tract of a healthy Mongolian child, and the collection number of the bifidobacterium lactis V9 is CGMCC No.5470. High-density fermentation of the bacteria of the bifidobacterium lactis V9 is realized under the control of an optimized culture medium and optimized fermentation conditions; the viable count in a fermentation liquor can be more than 2.0*10<10>cfu/ml, and the viable count in the freeze-dried powder of the bifidobacterium lactis prepared by using the fermentation liquor can be more than 6.0*10<11>cfu/g, and therefore, the freeze-dried powder of the bifidobacterium lactis can be applied to a probiotic leavening agent or a probiotic preparation.

The present invention discloses one plant lactobacillus strain and its application. The plant lactobacillus, Lactobacillus plantarum L323 CGMCC No. 1329, is separated from Chinese pregnant woman's vaginal secretion, and has relatively high bacteriotasis on common vaginal pathogens, such as staphylococcus aureus, candida albicans, colibacillus and vaginal Gardnar bacillus, and acid producing and H2O2 producing capacity higher than other lactobacillus. The plant lactobacillus, Lactobacillus plantarum L323 CGMCC No. 1329, may be used in preparing vaginal microbial preparation for preventing and/or treating vaginal infectious diseases.

The invention discloses a brown lactic acid bacteria beverage containing enzyme and a preparation method thereof. The lactic acid bacteria beverage comprises the following raw materials by mass percentage: 20-41% of raw material milk, 3-20% of fruit and vegetable juice, 0.15-0.5% of a stabilizing agent, 1.51-16% of a sweet substance, 0.05-0.35% of an acidity conditioning agent, 0.002-0.08% of buffer salt, 0.045-0.6% of a starter A, 0.001-0.004% of a starter B, and the balance of water. The preparation method comprises the following steps: by screening specific lactic acid bacteria, fermenting the fruit and vegetable juice to prepare a fruit and vegetable broth. The fruit and vegetable broth and a brown lactic acid bacteria beverage semi-finished product are mixed, the steps of blending, sterilization, aseptic homogenizing, and cooling are carried out to prepare the beverage with high live bacteria number, the brown lactic acid bacteria beverage has cow's milk fermentation flavor and special fermentation flavor of the fruit and vegetable juice, and has dual nutrition value of the enzyme and the fermented cow's milk.

The invention discloses a fermentation bacteria agent and a fermentation method of excrements of livestock and poultry. The fermentation bacteria agent of the excrements of livestock and poultry has the living bacteria concentration of more than or equal to 2 billion/ ml in the bacteria liquid; the living bacteria comprise the components in percentage based on number: 15-25% of low-temperature fermentation bacteria, 25-35% of medium-temperature fermentation bacteria and 45-55% of high-temperature fermentation bacteria. The fermentation bacteria agent is high in living bacteria content; the principle that different fermentation bacteria are started for fermentation at different temperatures when the low-temperature fermentation bacteria, the medium-temperature fermentation bacteria and the high-temperature fermentation bacteria are matched in different proportions is adopted, and aerobic fermentation is combined with anaerobic fermentation, so that equipment are not needed in the development and fermentation process, and the bacteria agent which can ferment under the natural conditions can be obtained. Compared with the prior art, the fermentation bacteria agent not only is rapid in fermentation speed so as to reduce the loss of nutrient substances of the excrements in the fermentation process, but also can dry the excrements by the temperature generated by fermentation, thus saving the energy consumption, and being low in cost, simple to operate and easy to popularize.

The invention discloses a compound preparation of piperacillin sodium and tazobactam sodium for injection, which comprises piperacillin sodium and tazobactam sodium by the weight ratio of 3-4:0.8-1.2. The preparation can be used for treating respiratory system infection, urological infection, otologia infection and skin infection.

The invention discloses a bio-organic fertilizer for dragon fruit planting and a preparing method thereof, and belongs to the technical field of fertilizer preparation. The bio-organic fertilizer for dragon fruit planting is prepared from, by weight, 40-60 parts of household garbage, 30-50 parts of animal waste, 20-30 parts of crop waste and 10-20 parts of tea seed meal, a complex microbial inoculant accounting for 0.5-2.5% of the total weight of the materials is added, first-time fermentation is carried out after uniform stirring, then effective microbial community accounting for 0.2-0.5% of the total weight of the materials is added, and the bio-organic fertilizer is obtained through second-time fermentation. According to the bio-organic fertilizer for dragon fruit planting, the microbial agent is added for scientific matching, the materials are fermented twice to be completely decomposed and degraded into nutrients easy to absorb and use or plants, and soil is loosened and nitrified, which is beneficial for absorbing the nutrients by crop root systems, so that the yield of dragon fruit is increased, and the quality of the dragon fruit is improved.

The invention relates to the food technology field, in particular to a yoghurt powder. The invention discloses a yoghurt powder which comprises the following components (part by weight): 60 parts to 80 parts of milk powder and 0.01 part to 0.04 part of probiotics powder. The viable count of the yoghurt powder is high. No thickener, essence and the like are needed to be added into the acidophilus milk fermented from the yoghurt powder, fermentation can be made at any time, and the yoghurt powder is natural, pure and rich in nutrition and can prevent diarrhea, astriction and the like from enhancing the intestine function of human body. Besides, the storage of the yoghurt powder is more convenient than the acidophilus milk sold in market and fermentation can be carried out at any time. In addition, the self-prepared acidophilus milk can be prepared into different tastes as one likes, thus being great pleasure and having great market value.

The invention discloses a preparation method of synbiotic goat milk powder, which comprises the following steps: activating probiotics to obtain probiotic mire; adding a compound freeze-drying protective agent; obtaining freeze-drying probiotic powder by freeze-drying; carrying out purification, standardization and reduced pressure condensation on a raw material of goat milk to obtain new raw material of goat milk; adding prebiotics in the condensed raw material of goat milk and then carrying out spray drying to obtain goat milk powder containing the prebiotics; cooling the goat milk powder to room temperature and filtering with a screen; and mixing the screened goat milk powder containing the prebiotics with the probiotic powder under the aseptic condition to obtain the symbiotic goat milk powder. In the invention, the prebiotics are added into condensed degrease goat milk by condensation or whole goat milk and are beneficial to the uniform dispersion of the prebiotics; the probiotics are prepared by a freeze-drying method and added with the freeze-drying protective agent, so that the viable bacteria is more and bacteria has a colony of over 1,010cfu/g; and by adding the prebiotics into the goat milk powder, the prebiotics can be protected and the survival rate of the prebiotics and the functionality of the goat milk powder can be improved.