22results about How to "High viable count" patented technology

The invention relates to a soil conditioner for saline-alkali land treatment and a preparation method, and belongs to the technical field of soil conditioners. The soil conditioner for saline-alkali land treatment is prepared from a fermented fertilizer, biomass charcoal II, corn straw powder, attapulgite powder, humic acid and a compound microbial agent. The soil conditioner disclosed by the invention can be used for reducing the pH value of soil, reducing salinity, promoting the recovery of organic matters and improving the fertility of the soil, and is beneficial to improving the yield of crops.

The invention belongs to the technical field of microbial fermentation, and particularly relates to a fermentation substrate for bacillus, a bacillus liquid fermentation inoculant and application. The fermentation substrate is prepared by adding traditional Chinese medicine residues into a yeast culture medium so as to improve the concentration of bacillus after fermentation; the traditional Chinese medicine residues are prepared by compounding motherwort herb residues, astragalus membranaceus herb residues and ginseng herb residues according to the mass ratio of 1: (1-2): (1-1.5); the mass ratio of the yeast culture medium to the traditional Chinese medicine residues is (20-26): (6-8). The concentration of the bacillus can reach 3.3 * 10 < 9 > CFU / mL in a bacillus liquid fermentation inoculant obtained by adopting the fermentation substrate disclosed by the invention.

The invention discloses a synthetic functional bacterial agent and application of the synthetic functional bacterial agent in salt tolerance and growth promotion of wheat, the synthetic functional bacterial agent is prepared from five strains, namely, Gordonia hydrophora, Lysinibacillus oldseyi, planomicrobium okeanokites, Luteimonas huaabiensis and Brevibacterium epidermidis, and the bacterial agent has the remarkable characteristics of no antagonism among the strains, salt and alkali tolerance, high viable count and the like, and can be applied to salt tolerance and growth promotion of wheat. The germination rate, the seedling length and the root length of the spring wheat can be remarkably increased, and the seedling length and the root length of the winter wheat are also remarkably promoted. Under the condition of salt stress, the microbial inoculum can remarkably improve the activity of catalase, peroxidase and superoxide dismutase of wheat and the total content of chlorophyll, after microbial inoculum treatment, the content of malondialdehyde and proline of wheat is remarkably reduced, and the microbial inoculum plays a role in promoting and protecting spring wheat and winter wheat in the process of resisting saline-alkali stress and has a good application prospect. The method has an influence effect on agricultural development and sustainable development in the arid region.

The invention relates to a microbial water body substrate modifier and application thereof. The microbial water body substrate modifier comprises the following components: compound microbial powder, zeolite powder, shell powder and polyferric sulfate, the compound microbial powder comprises bacillus powder and lactic acid bacteria powder. The microbial water substrate modifier can effectively realize rapid bottom modification of aquaculture water, improve water quality, optimize aquaculture environment, reduce diseases and promote healthy growth of aquaculture animals. Wherein the bacillus can flocculate and settle residual organic matters such as feed, animal waste and the like in the culture water body by utilizing the flocculation effect of the bacillus, and the physical adsorption components, namely the zeolite powder and the shell powder, and the chemical adsorption component, namely the polyferric sulfate are synergistically matched, so that suspended organic matter large particles in the water body are effectively adsorbed and settled; and the organic matters are jointly degraded through the common graded degradation effect of the bacillus and the lactic acid bacteria, so that the effects of improving the substrate of the aquaculture water body and promoting the healthy growth of the aquaculture animals are achieved.

The application discloses a preparation method and application of low-protein probiotic fermented feed, relates to the technical field of fermented feed and low-protein daily ration, and comprises the following steps: S1, preparing a low-protein fermented base material, wherein the low-protein fermented base material comprises, in mass parts, 45-70 parts of cereal raw materials, 6-22 parts of plant protein by-products, 8-28 parts of bran fiber raw materials, 1-6 parts of fermentable sugar sources, 0.2-2.0 parts of mineral buffer components and 0.15-2.50 parts of amino acid balancing components, and the crude protein content of the low-protein fermented base material is controlled to be 10.0-16.5 wt% on a dry basis; and S2, performing steam conditioning or wet heat curing treatment on the low-protein fermented base material after being crushed and mixed to obtain a pretreated base material. Through the combination of the low-protein base material design and the amino acid balancing components, the crude protein content of the product is stably controlled to be 10.0-16.5 wt%, and meanwhile, the limiting amino acids such as lysine, methionine, threonine, tryptophan, valine and isoleucine are supplemented, so that the problem of amino acid mode imbalance caused by the reduction of protein raw materials is avoided.

This invention provides a fermented milk yogurt for infants, which, by weight, consists of the following ingredients: 300-450 parts fermented milk, 80-120 parts compound fresh fruit puree, 20-40 parts concentrated fruit juice, 10-20 parts compound dietary fiber, 15-30 parts protein components, and 5-15 parts cereal powder. This invention introduces five specific proportions of compound probiotics for fermentation, including *Bifidobacterium animalis* subsp. *lactobacterium* HN019, *Lactobacillus fermentum* CECT5716, *Lactobacillus rhamnosus* HN001, *Bifidobacterium breve* M-16V, and maltodextrin. These five strains produce a significant synergistic effect, not only shortening the fermentation time and increasing the number of live bacteria in the fermented milk, but also forming a more complete intestinal flora barrier in infants, effectively promoting nutrient absorption and reducing the incidence of diarrhea and constipation in infants.

The invention relates to the technical field of microbial preparation fermentation processes, in particular to a bifidobacterium and clostridium butyricum liquid-state combined fermentation culture medium and a fermentation method. The combined fermentation culture medium comprises a base material culture medium and a supplemented material culture medium, the base material culture medium comprises 5-10 g / L of yeast extract powder, 20-40 g / L of bean flour hydrolysate, 2-5 g / L of glucose, 10-20 g / L of sucrose, 1-5 g / L of compound oligosaccharide, 1-5 g / L of dipotassium phosphate, 1-5 g / L of monopotassium phosphate, 1-3 g / L of magnesium sulfate, 1-5 g / L of calcium carbonate, 1-3 g / L of sodium acetate, 0.1-0.5 g / L of manganese sulfate, 1-3 g / L of potassium citrate and 0.5-5 g / L of deoxidant. The co-fermentation culture medium can meet the requirement of symbiosis of bifidobacterium and clostridium butyricum on nutrient substances, co-fermentation of bifidobacterium and clostridium butyricum is achieved, and the viable count per unit volume is increased.

The invention relates to the technical field of microorganisms, and discloses a plant lactobacillus IMAUJBP6 strain and application thereof. The plant lactobacillus IMAUJBP6 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.36779, and the preservation date is November 26, 2025. The plant lactobacillus IMAUJBP6 obtained by screening can play an anti-oxidation role by inhibiting the activity of alpha-amylase and alpha-glucosidase and improving the DPPH free radical scavenging rate, the superoxide anion free radical scavenging rate and the hydroxyl free radical scavenging rate at the same time, so that the effect of reducing blood sugar is synergistically achieved. When the strain is applied to preparation of fermented milk, a fermented milk product with excellent quality can be obtained, especially when camel milk is used as a raw material for fermentation, the product shows a remarkable potential probiotic effect in the aspects of reducing blood sugar and resisting oxidation, and a new strain and a technical support are provided for development of functional fermented milk products and products for reducing blood sugar and resisting oxidation.

The present application relates to the technical field of fermentation medium, more specifically, it relates to a high-density fermentation medium for streptococcus suis and preparation and application thereof.The medium comprises beef infusion powder 1-5 parts, tryptone 1-10 parts, pancreatic casein peptone 1-10 parts, soybean peptone 1-5 parts, dipotassium hydrogen phosphate 1-5 parts, sodium chloride 1-10 parts, yeast extract 1-10 parts and glucose 1-5 parts.

The invention relates to the technical field of microbial fermentation, and discloses a method for producing selenium-rich protein feed by multi-bacteria synergistic fermentation of Hami melons, which comprises the following steps: crushing Hami melons, mixing with a carbon source and a nitrogen source, inoculating a leavening agent, and fermenting; and drying and crushing the fermented product to obtain the protein feed. The leavening agent comprises candida utilis, aspergillus niger and bacillus licheniformis; the candida utilis culture medium comprises the following components: 9.56 g / L of yeast extract, 19.86 g / L of glucose, 1.45 g / L of dipotassium phosphate, 1.12 g / L of magnesium sulfate, 25 mg / L of sodium selenite, L-lysine and vitamin B1, and the mass concentration ratio of the sodium selenite to the L-lysine to the vitamin B1 is 1: (116-232): (0.06-0.12). According to the invention, the cantaloupe is cooperatively fermented by adopting multiple bacteria, so that the selenium-enriched protein feed is obtained.

The invention discloses a growth-promoting fermentation aid for lactobacillus rhamnosus fermented milk and an application thereof. The fermentation aid comprises glutamic acid, glutamine, aspartic acid, asparagine, alanine, calcium pantothenate, thiamine, uracil, manganese sulfate, thymine, guanine and adenine in a mass ratio of 1: (0.15-0.2): (0.1-0.2): (0.2-0.3): (0.1-0.2): (0.1-0.15): (0.0015-0.0025): (0.02-0.03): (0.01-0.02): (0.02-0.03): (0.02-0.025): (0.015-0.02). The fermentation aid can shorten the fermentation time of lactobacillus rhamnosus fermented milk, while greatly increasing the number of live bacteria, thereby solving the problem of a long fermentation cycle of lactobacillus rhamnosus fermented milk.

The invention discloses a fermentation method of an alcohol decomposition probiotic combination and the probiotic combination. The fermentation method comprises the following steps: mixing seed solutions of lactobacillus plantarum, lactobacillus rhamnosus and lactobacillus paracasei, inoculating a fermentation medium with the mixed seed solutions, and fermenting; the fermentation temperature is 28-35 DEG C, and the fermentation time is 40-60 hours. The lactobacillus plantarum, the lactobacillus rhamnosus and the lactobacillus paracasei are creatively subjected to mixed fermentation, the mixed fermentation can generate a synergistic effect, the biomass of a fermentation product is increased in unit time, and a foundation is laid for wide application in industrial production.

The invention relates to the technical field of fermented milk products, in particular to fermented milk containing bifidobacteria with high viable count and a preparation method of the fermented milk. Specifically, when the fermented milk containing the probiotics is prepared, raw milk, white granulated sugar, wheat hydrolyzed protein powder, inulin and milk protein powder are jointly used as raw materials of the fermented milk, and the viable count of the probiotics bifidobacterium in the fermented milk is increased by using the wheat hydrolyzed protein powder, the inulin and the milk protein powder according to the mass ratio of (1-4): (1-10): (1-8). By adding the prebiotics, the milk protein powder and the plant protein powder composition into the formula, the bifidobacterium fermented milk has a remarkable value-increasing effect, and the problem that the viable count of the bifidobacterium-containing probiotic fermented milk is low is effectively solved.

The application discloses a DPP-IV inhibiting peptide-producing lactobacillus helveticus, a goat milk with blood sugar reduction and a preparation method thereof, and relates to taking goat milk as raw material, fermenting the goat milk by using the DPP-IV inhibiting peptide-producing lactobacillus helveticus KD12 to obtain the goat milk fermented by the lactic acid bacteria containing DPP-IV inhibiting peptide with blood sugar reduction, and preparing the goat milk powder fermented by the lactic acid bacteria containing DPP-IV inhibiting peptide with blood sugar reduction through drying after fermentation. The lactic acid bacteria of the goat milk fermented by the lactic acid bacteria with blood sugar reduction is inactivated to obtain the goat milk with postbiotics with blood sugar reduction, and after concentration, the goat milk powder with postbiotics with blood sugar reduction is obtained through drying. The goat milk with blood sugar reduction provided by the application has a high DPP-IV inhibition rate, can be used for inhibiting DPP-IV activity after being eaten by consumers, thereby reducing blood sugar, reducing the dosage of blood sugar reduction drugs, and thereby reducing the side effects caused by the blood sugar reduction drugs.

The invention provides a Lawrence estuarine Pseudomonas D13 and an application thereof. The Pseudomonas D13 has a strong ability to degrade n-hexanal and can effectively remove n-hexanal. The bacterium also has good acid-base tolerance and osmotic pressure tolerance and can grow and reproduce in extreme acid-base stress environments and salt stress environments.

The invention relates to a high-density and high-activity mixed bacteria fermentation method and a product and application thereof, and the method comprises the following steps: inoculating a same and different lactic acid bacteria mixed strain seed solution into a fermentation medium, and fermenting to obtain the high-density and high-activity mixed bacteria, the mixed strains of lactic acid bacteria of the same kind and different strains comprise mixed strains of streptococcus thermophilus of the same kind and different strains, mixed strains of lactobacillus delbrueckii subspecies bulgaricus of the same kind and different strains or mixed strains of lactobacillus acidophilus of the same kind and different strains. According to the fermentation method disclosed by the invention, the yield and the viable count of the bacterial powder are improved, the production efficiency is improved, the cost is reduced, and the fermentation activity of the obtained bacterial powder is stronger than that of single-bacterium fermentation.

The invention discloses a fermentation aid for animal bifidobacterium single bacteria fermented milk and an application thereof. The fermentation aid comprises a fermentation aid for promoting the growth of animal bifidobacterium, and specifically comprises glutamine, arginine, asparagine, threonine, serine, alanine, valine, methionine, leucine, lysine, ascorbic acid, guanine and uracil in a mass ratio of (0.1-0.15): (0.9-1.0): (0.3-0.4): (0.1-0.2): (0.1-0.2): (0.1-0.2): (0.2-0.3): (0.1-0.2): (0.2-0.25): (0.2-0.35): 1: (0.01-0.03): (0.01-0.03). The fermentation aid can shorten the fermentation time of animal bifidobacterium single bacteria fermented milk, while greatly increasing the number of live bacteria, thereby solving the problem of a long fermentation cycle of animal bifidobacterium single bacteria fermented milk.

The application relates to the field of biological medicine, and discloses a bifidobacterium adolescentis YF-01 and application of the bifidobacterium adolescentis YF-01 in fermented milk. The bifidobacterium adolescentis YF-01 is isolated from adult feces and is preserved in the China Center for Type Culture Collection, with a preservation number of CCTCC NO: M 20251308. Experiments prove that the bifidobacterium adolescentis YF-01 has good gastrointestinal fluid tolerance, the survival rate can reach 90.1% after being treated by simulated gastric juice for 3 hours, and the survival rate can reach 72% after being treated by simulated intestinal juice for 4 hours. The self-aggregation rate of the strain is 88.83%, and the hydrophobicity is 79.41%; the strain has significant scavenging capacity for DPPH free radicals and hydroxyl free radicals, and shows good antioxidant activity and adhesion capacity, which provides a theoretical basis for the application of the strain in fermented milk. The strain and metabolites thereof can be used for preparing food, medicine or health care products with the functions of regulating intestinal flora and enhancing antioxidant capacity, and have significant application potential in the development of functional fermented milk products.

The application belongs to the technical field of utilization of waste biomass by microorganisms, and specifically discloses an abnormal williamsonia strain Y8 and application thereof in beer dreg fermentation. The preservation number of the strain is CCTCC NO: M 2026579. The strain has good tolerance to extreme chemical environments such as high salt, high sugar, strong acid and high-concentration ethanol; can ferment polysaccharides and monosaccharides; under the condition of rich nutrition, the content of cell protein can reach 57%; can adapt to the liquid physicochemical environment of beer dregs and effectively utilize beer dregs to grow. With the aid of enzyme preparation composed of beta-glucanase and xylanase, the efficiency of the strain in utilizing beer dregs to grow is significantly improved, and after 72 h of fermentation, the fermentation liquor with viable cell count of the strain ≥1.8×10 9 CFU / mL is obtained, and the prepared fermentation feed is effectively enriched with total phenols and improved in antioxidant performance. The application is green, mild and efficient, and has excellent application prospect.

The invention belongs to the technical field of microbial fermentation, and particularly relates to a method for promoting the activation speed of feed lactic acid bacteria. A carbon source and a nitrogen source rapidly activated by lactic acid bacteria are screened, and the adding proportion of the carbon source and the nitrogen source and the fermentation process are determined through a central combination experiment. Finally, the bacterial powder, the brown sugar, the pichia pastoris hydrolysate and the tap water are heated and activated for 12-24 hours in a water bath according to a certain proportion, so that the viable count of the lactic acid bacteria can reach 9.8 E + 08 CFU / m and is increased by 80-100 times compared with other carbon and nitrogen source culture. Therefore, the practical value is high.

The invention relates to the technical field of biological feed preparation, in particular to a method for preparing fermentation liquor rich in organic acid from garlic syrup. According to the method disclosed by the invention, the dominant flora obtained by self-fermentation of the garlic syrup is adopted for fermentation of the organic acid, the garlic syrup containing garlic oil or allicin can be used as a fermentation raw material of the organic acid, allicin removal treatment does not need to be carried out on the raw material, and the preparation process of the organic acid is simplified; the viable count of the fermentation seed solution enriched by the method is high, and higher saccharide conversion efficiency can be realized; the fermentation liquor prepared by the method has higher organic acid content, and the garlic syrup can be processed into a valuable organic acid compound, so that resource utilization is realized.

This invention discloses a method for preparing a *Lactobacillus plantarum* enrichment agent and its application, relating to the field of microbial technology. The preparation method includes: extracting traditional Chinese medicine to obtain medicinal residue; enzymatically hydrolyzing the residue; adjusting the pH to neutral to obtain the enrichment agent; wherein the traditional Chinese medicine is selected from one or more of the following: dried tangerine peel, white hyacinth bean, coix seed, polygonatum, lotus leaf, dendrobium officinale, licorice, atractylodes macrocephala, poria cocos, chicken gizzard lining, roasted malt, wolfberry, kudzu root, and clove. The *Lactobacillus plantarum* enrichment agent provided by this invention uses medicinal residue as raw material and is specifically processed using a compound enzyme. It has low raw material costs, high product added value, and can significantly increase the viable count of *Lactobacillus plantarum* in a unit volume of fermentation broth, effectively reducing the cost of preparing highly active *Lactobacillus plantarum* inoculants.