Plant lactobacillus strain and its application

A technology of Lactobacillus plantarum and drugs, applied in bacteria, drug combinations, medical raw materials derived from bacteria, etc., can solve the problems of unsuitability for preparations, slow growth, and different nutritional needs of lactobacilli.

Inactive Publication Date: 2007-01-03
TIANYOUDA BIO ENG SCI & TECH BEIJING +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0031] The growth of Lactobacillus has very different nutrient requirements. Most Lactobacillus have strict nutritional requ

Method used

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  • Plant lactobacillus strain and its application
  • Plant lactobacillus strain and its application
  • Plant lactobacillus strain and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1. Isolation and Identification of Lactobacillus plantarum L323 CGMCC No.1329

[0044] 1. Isolation of Lactobacillus plantarum L323 CGMCC No.1329

[0045] Firstly, the vaginal vaginal fornix secretions of more than 300 healthy Chinese pregnant women undergoing regular pregnancy checkups in the Obstetrics and Gynecology Department of the 301 Hospital were collected, and the following tests were carried out while using MRS medium for bacterial isolation: (1) Direct smear , according to the number of lactobacilli and gram-negative bacteria to evaluate the cleanliness (0-2 points, normal; 4-6 points, mild inflammation; 8-10 points, severe inflammation), among them, the evaluation criteria of vaginal cleanliness Reference literature (Robert P.Nugent, Marijane A.Krohn, and Sharon L.Hillier Reliability of diagnosing bacterial vaginosis is improved by a standardized method of Gram stain interpretation Journal of Clincal Microbiology1991, 29(2):297-301); (2) vagina Secret...

Embodiment 2

[0051] Embodiment 2, the characteristic of plant Lactobacillus (Lactobacillus plantarum) L323 CGMCC No.1329

[0052] Material

[0053] Strains Staphylococcus aureus ATCC6035, Candida albicans ATCC1023, Escherichia coli ATCC25922, Gardnerella vaginalis ATCC14018

[0054] Reagent

[0055] MRS medium and LM broth medium were purchased from Mérieux Biological Company of France;

[0056] Tryptone, yeast extract, and agar powder were purchased from Oxide;

[0057] Tetramethylbenzidine (TMB), dimethylformamide, and horseradish peroxidase (HRP) were purchased from Sigma.

[0058] 1. Antibacterial test

[0059] Lactobacillus plantarum (Lactobacillus plantarum) L323 CGMCC No.1329 strain was inoculated in MRS medium at 37°C, 5% CO 2 Cultivate for 24 hours, wash the bacterial lawn with sterile physiological saline, turbidity and dilute to 1×10 8 CFU / ml concentration of bacterial liquid, soak a circular sterile filter paper piece with a diameter of 5mm in the bacterial liquid and set...

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Abstract

The present invention discloses one plant lactobacillus strain and its application. The plant lactobacillus, Lactobacillus plantarum L323 CGMCC No. 1329, is separated from Chinese pregnant woman's vaginal secretion, and has relatively high bacteriotasis on common vaginal pathogens, such as staphylococcus aureus, candida albicans, colibacillus and vaginal Gardnar bacillus, and acid producing and H2O2 producing capacity higher than other lactobacillus. The plant lactobacillus, Lactobacillus plantarum L323 CGMCC No. 1329, may be used in preparing vaginal microbial preparation for preventing and/or treating vaginal infectious diseases.

Description

technical field [0001] The invention relates to a strain of Lactobacillus and its application, in particular to a strain of Lactobacillus plantarum and its application. Background technique [0002] 1. Lactobacillus and women's vaginosis [0003] Lactobacillus (Lactobacillus) is the main and most important normal flora in the vagina of healthy women. Lactobacillus in the vagina is not only non-pathogenic, but also can self-clean the micro-ecological environment of the vagina and restrict the colonization and growth of other pathogenic bacteria. Therefore, clinically, a large number of lactobacilli (several per field of view under a high-power microscope) can be seen in vaginal smears, and no or only a small amount of Gram-negative bacilli can be seen as the standard of normal and no vaginal infection, while lactobacilli in the vagina are absent Reliability of diagnosing bacterial vaginosis improved by a standardized method of Gram stain interpretation Journal of Clincal Mic...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/74A61P15/02A61K35/747
Inventor 陈薇张俊端青曹琇蓉陈浪
Owner TIANYOUDA BIO ENG SCI & TECH BEIJING
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