196 results about "Lactobacillus fabifermentans" patented technology

According to the invention there is provided a strain of lactic acid bacteria of the genus Lactobacilli obtainable by isolation from resected and washed feline gastrointestinal tract having a probiotic activity in animals. Methods of use and compositions comprising the Lactobacilli of the present invention are also provided.

Present invention features a novel use of Lactobacillus for treating type 2 diabetes, wherein the Lactobacillus is Lactobacillus reuteri GMNL-89 with the deposition numbers of BCRC 910340 and CCTCC M 207154 and comprises of live, dead bacteria or supernatant of the bacterial culture(s) and a pharmaceutically acceptable vehicle. The invention also discloses the efficacies of the indicated Lactobacillus strain including anti-oxidative enzyme activity, anti-inflammation, reduction of blood glucose level, reduction of triglycerides, reduction of total cholesterol, reduction of LDL, increase of HDL and improve insulin utilization.

The invention provides a micro-ecological fermented protein feed and a preparation method, wherein the feed is prepared by mixing bean pulp and silkworms with quality ratio 90-98:2-10, then cooking to obtain solid state fermention medium, then inoculating mixed strains of bacillus subtilis, Saccharomyces cerevisiae, lactobacillus and Rhizopusoligosporus on solid fermention medium, then co-mixing for 30-45 hours at 33-37 DEG C, drying fermentation products after completing fermenting, crushing and obtaining the micro-ecological fermented protein feed. The inventive micro-ecological fermented protein feed contains a plurality of biological active peptides, content of small peptide reach more than 22%, which is favourable for digestion and development; contains lactobacillus, microzyme, which is favourable for controlling useful microbiological population in intestinal tracts of animals; has good delicious flavor and good luring effect, advances young animals for ingestion; contains soybean isoflavone more than 0.05%, markedly increases immunity of bodies, increases producibility of animals; relieving odmour of fecal waste; fully takes place of animal protein feed of fish meal and plasma protein flour and the like.

Sterilization methods and apparatus are effective to achieve a 6-log reduction in CFUs of industry standard bacteria and bacterial spores, i.e., B. stearothermophilus and B. subtilis spores, by subjecting sterilizable materials to a chemical additive-containing carbon dioxide sterilant fluid at or near its supercritical pressure and temperature conditions. Most preferably, the chemical additive-containing supercritical carbon dioxide sterilant fluid is agitated during sterilization, e.g., via mechanical agitation or via pressure cycling.

The solid fermentation process for producing natto bacillus feed additive with bean dregs includes the following steps: 1. shake flask culture of natto bacillus in KMB culture medium to obtain the first stage spawn; 2. dissolving bean dreg powder, corn starch, corn slurry and NaCl in water, regulating pH value, sterilizing and inoculating the first stage spawn, and shake flask culture to obtain the second stage spawn; 3. mixing water soaked bean dregs, NaCl, glucose as the carbon source, gelatin as the nitrogen source and (NH4)2SO4 or urea, and sterilizing to obtain solid culture medium, inoculating the second stage spawn, fermenting while maintaining the temperature and humidity to obtain fermented bean dregs; and 4. adding sterilized corn powder into the fermented bean dregs, drying and crushing to obtain the natto bacillus feed additive. The feed additive can promote the growth of pig, promote the growth of lactobacillus and inhibit colibacillus.

The present invention discloses one plant lactobacillus strain and its application. The plant lactobacillus, Lactobacillus plantarum L323 CGMCC No. 1329, is separated from Chinese pregnant woman's vaginal secretion, and has relatively high bacteriotasis on common vaginal pathogens, such as staphylococcus aureus, candida albicans, colibacillus and vaginal Gardnar bacillus, and acid producing and H2O2 producing capacity higher than other lactobacillus. The plant lactobacillus, Lactobacillus plantarum L323 CGMCC No. 1329, may be used in preparing vaginal microbial preparation for preventing and / or treating vaginal infectious diseases.

The present invention provides Lactobacillus plantarum CJLP55 KCTC 11401BP, a composition for treating bowel disease containing the lactobacillus, and a composition for immunological enhancement containing the lactobacillus.

The present invention relates to four kinds of lactobacilli, including Lactobacillus gasseri with preservation number of CGMCC No. 0840, Lactobacillus reuteri with preservation number of CGMCC No. 0841, Lactobacillus acidophilus with preservation number of CGMCC No. 0842 and Lactobacillus fermentum with preservation number of CGMCC No. 0843, separated from piglet intestinal tract and bred. The combination of the four kinds of lactobacilli is used in preparing the probiotics preparation for preventing and treating piglet ablactation diarrhoea and promoting growth, and the probiotic preparation is added into feed for ablactated piglet to prevent and treating diarrhoea and promote its growth.

A probiotic composition used for livestock and poultry can overcome micro dysbiosis, decreased disease resistance and production performance of livestock and poultry, and the side effects like drug resistance and drug residue which are caused by using antibiotics and chemical drugs in a large amount for a long time in feed. The probiotic composition contains effective components with the following weight portions: 3 to 10 portions of Lactobacillus acidophilus, 2 to 6 portions of yeast, 1 to 8 portions of bacillus subtilis, 3 to 8 portions of Bifidobacterium longum, 2 to 5 portions of bacillus licheniformis, and 1 to 4 portions of aspergillus oryzae and vector. The weight ratio of probiotic to vector is 2:7, and the content of the active compositions are that: greater than or equal to 8 billion / g of Lactobacillus acidophilus, greater than or equal to 7 billion / g of yeast, greater than or equal to 9 billion / g of bacillus subtilis, greater than or equal to 9 billion / g of Bifidobacterium longum, greater than or equal to 6 billion / g of bacillus licheniformis and greater than or equal to 6 billion / g of aspergillus oryzae, respectively. The probiotic composition of the invention can reduce lead content in animal products, achieve the purpose of producing green and harmless animal products, promote growth and development of animal and increase animal digestibility to feed.

The invention relates to a method for preparing a functional microbial preparation from lactic acid bacillus and yeast, which is characterized in that: liquid of beneficial strains is adopted for culture; cell walls of the strains are collected centrifugally; a strain group is subjected to solid amplification culture; and a culture is added with the collected cell walls rich in polysaccharide. The method has the advantages that: high-concentration lactobacillus in the product can obviously improve the digestion and absorption of bred animals, and the generated metabolic products such as peptidogly can improve the immunity of the animals to pathogen; the high-concentration yeast in the product can improve rich single cell proteins required by the growth of the animals, and simultaneously the yeast cells are subjected to full wall breaking, and release a great amount of beta-1,3-glucan so as to obviously improve the immunity to Gram positive bacteria and Gram negative bacteria; and grain crops and common feeds are adopted as main raw materials for a culture medium, the production cost is reduced, and the method is easy to promote in animal breeding.

According to the present invention there are provided methods of use in companion animals of probiotic bacteria of the genus Lactobacilli.

The invention relates to multi-bacterium microbial compound preparations for aquaculture and a preparation method thereof. The multi-bacterium microbial compound preparations are characterized by comprising the following steps of: preparing seed culture solution by performing single strain culture on rhodoplanes elegans, pichia fermentans lodder, bacillus natto swamura, bacillus subtills, lactococcus lactis and bifidobacterium sp respectively; inoculating the seed culture solution onto the same culture medium; and then culturing the seed culture solution by a fermentation tank, and naturally reducing a pH value of the seed culture solution to 3.0 to 3.5 to obtain the multi-bacterium microbial compound preparations, wherein the bacterial content of the pichia fermentans lodder in the preparations is more than or equal to 0.1*10<9>cfu / ml; the bacterial content of the other strains is more than or equal to 5.0*10<9>cfu / ml. The multi-bacterium microbial compound preparations for aquaculture can improve the ecological environment of aquatic animals, prevents and treats diseases, reduces the emergency condition of the animals, improves the quality of the aquatic animals, and shortens a listed period.

The invention provides a method for producing fermented soybean meal by two-step inoculation segmented solid-state fermentation. The method comprises the following steps of: inoculating the mixed bacterial solution obtained by mixing bacillus subtilis solution, candida mycoderma bacteria solution and lactobacillus solution in peeled soybean meal and performing mixed fermentation; and inoculating the lactobacillus solution in the fermented product, mixing, and performing anaerobic fermentation to obtain the fermented soybean meal. The method is capable of meeting the different needs of aerobic bacteria and anaerobic bacteria, can be used for preparing high-quality fermented soybean meal, and is free of chemical additives; due to the various digestive enzymes, organic acids, medium and small molecular proteins (functional peptides), amino acids, vitamins, probiotics and the like generated during the fermentation process, the nutritive value of the soybean meal is greatly increased; the prepared fermented soybean meal contains not less than 16% of soybean peptide, not more than 25mg / g of glycinin, not more than 20mg / g of beta-conglycinin, not less than 3.5% of lactic acid, and not less than 2*10<9> beneficial viable bacteria; and microbial source nutrients are beneficial to the maintenance of intestinal structure and can promote immunologic function and improve animal production performance.

A strawberry probiotic fermented drink is characterized by using fresh strawberry juice to produce a culture medium, fermenting and culturing such probiotics as bifidobacterium, lactobacillus bulgaricus, streptococcus thermophilus and lactobacillus under certain temperature and time, and then inactivating the fermented probiotic liquid to produce the drink. The residues produced in the process of producing the fresh strawberry probiotic fermented drink can be used for producing strawberry probiotic jams. The invention greatly expands the application range of the culture medium materials of the probiotics such as bifidobacterium, provides a novel probiotic inactivated fermented drink for the consumers and enjoys rich raw material sources, low cost, stable production process, small investment on production equipment, simple operation and easy popularization.

The invention provides a beneficial fungus feed additive for large-scale composite edible fungi and a method for breeding flavored pigs. The beneficial fungus feed additive is formed by compounding a beneficial fungus mixture and the large-scale edible fungi, wherein the beneficial fungus mixture consists of lactobacillus, streptococcus faecalis, saccharomycetes, bacillus subtilis, bacillus subtilis natto and bacillus licheniformis, and live bacterial counts of the beneficial fungus mixture range from 0.8 to 1 billion / gram; the beneficial fungus mixture and the crushed fresh large-scale edible fungi are mixed in a mass ratio of 1:(0.8-1.2), and are placing at the temperature of between 33 and 37 DEG C for 3 to 5 days to obtain the beneficial fungus feed additive for the large-scale composite edible fungi. The method for breeding he flavored pigs comprises the following steps of: adding the beneficial fungus feed additive for the composite edible fungi into feed in a ratio of 0.1 to 0.5 mass percent feeding the pigs before slaughter for 30 to 60 days and thus, obtaining green flavored pork products.

The invention discloses a panda source lactobacillus plantarum and application. The strain was preserved in China Center for Type Culture Collection on May 4, 2016 and the preservation number is CCTCC NO: M2016245. The panda source lactobacillus plantarum provided by the invention can be used for improving antibody levels of IgA, IgM and IgG immune globulins of blood serum; a condition that the percent of lymphocytes is reduced and the percent of neutrophils is increased, caused by infection and induction of enterotoxigenic escherichia coli, is inhibited; expression levels of inflammation related factors IL-1beta, IL-8, IL-6, TLR4 and MyD88mRNA (messenger Ribonucleic Acid) are remarkably down-regulated, and intestinal acute inflammation response caused by the enterotoxigenic escherichia coli is alleviated.

The invention relates to a porcine lactobacillus reuteri lyophilized preparation and a preparation method thereof, belonging to the field of application of microbes. The porcine lactobacillus reuteri lyophilized preparation is characterized in that a protective agent in the lyophilized preparation comprises the following ingredients in percentage by weight: 10 percent of skimmed milk, 0.1 percent of sodium glutamate, 0.3 percent of cysteine, 10 percent of fucose, 8 percent of lactose, 10 percent of dextrine and the balance of water. Proved by experiments, the porcine lactobacillus reuteri lyophilized preparation of the invention can increase the viable lactobacillus content in nursery pig feces, can reduce the content of colon bacillus, and has the effect of improving the average daily gain and immunity of the nursery pigs. The lyophilized preparation prepared by the method of the invention has the viable bacteria count not less than 109cfu / g, and the viable bacteria survival rate is higher than 75 percent after the lyophilized preparation is stored for 90 days. The invention also provides an optimized culture medium for lactobacillus reuteri, and the viable bacteria count is six times higher than that that of the viable bacteria cultured on the conventional culture medium (Magnetic Resonance Spectrum (MRS) culture medium).

The invention relates to a recombinant Lactobacillus strain, with limited growth and viability in the environment. More particularly, it relates to a recombinant Lactobacillus that can only survive in a medium where thymidine is present. By this strict dependency upon thymidine, thymidineless death is rapidly induced in this recombinant strain. A preferred embodiment is a Lactobacillus that may only survive in a host organism where thymidine is present, but cannot survive outside the host organism in absence of this medium compound. Moreover, the Lactobacillus strain can be transformed with prophylactic and / or therapeutic molecules and can, as such, be used to treat diseases such as, but not limited to, inflammatory bowel diseases.

Sterilization methods and apparatus are effective to achieve a 6-log reduction in CFUs of industry standard bacteria and bacterial spores, i.e., B. stearothermophilus and B. subtilis spores, by subjecting sterilizable materials to a chemical additive-containing carbon dioxide sterilant fluid at or near its supercritical pressure and temperature conditions. Most preferably, the chemical additive-containing supercritical carbon dioxide sterilant fluid is agitated during sterilization, e.g., via mechanical agitation or via pressure cycling.

A Lactobacillus paracasei-containing product used for inhibiting dental diseases caused by bacteria is provided, which comprises a plurality of Lactobacillus paracasei for inhibiting the growth of bacteria of dental diseases. Particularly, foods, oral hygiene products or oral treatment medicine containing the Lactobacillus paracasei when being administered or applied to a user can inhibit or reduce the number of pathogens of dental caries and periodontal diseases in oral cavity of the user, thereby achieving the efficacy of preventing dental diseases, such as dental caries and periodontal diseases.

The present invention relates to a microorganism belonging to the group of lactic acid bacteria characterized in that it is capable of specifically binding to Streptococcus mutans, wherein the specific binding is (i) resistant to heat treatment; and / or (ii) resistant to protease treatment; and / or (iii) calcium-dependent; and / or (iv) formed within a pH range between 4.5 and 8.5; and / or (v) formed in the presence of saliva. Preferably, the specific binding can be assayed as follows: (a) growing said microorganism to stationary phase; (b) mixing said microorganism with Streptococcus mutans which has been grown to stationary phase; (c) incubating the mixture obtained in step (b) under conditions allowing the formation of aggregates of said microorganism and Streptococcus mutans and (d) detecting aggregates by the occurrence of a pellet. Another aspect of the present invention is an analog or fragment of said microorganism which is thermally inactivated or lyophilized, wherein said analog or fragment retains the capability of specifically binding to Streptococcus mutans. In addition, the present invention encompasses compositions and additives for food, feed or drinks comprising the microorganism belonging to the group of lactic acid bacteria which specifically bind to Streptococcus mutans or an analog or fragment thereof. Moreover, uses of said microorganism or said analog or fragment thereof for the preparation of an anticariogenic or pharmaceutical composition or anticariogenic food or feedstuff as well as methods for producing said compositions or food or feedstuff are provided by the present invention.

The invention provides Lactobacillus salivarius M6, which is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the number of CGMCC 3505. The invention also provides a lactobacillus which can be mixed with animal feed or added into water, can be used together with antibiotics at the same time, and has antibiotic resistance. The strain can inhibit growth of Salmonella enterica, Escherichia coli and other pathogenic bacteria. The antibacterial composition of the Lactobacillus salivarius M6 can be used in drinking water, drinking water additives, feed and food additives of animals, human and animal medical composition, as well as drinks, drinking additives, foods, food additives and the like of humans. By implementation of the invention, pathogenic bacterium infection of animals or humans can be effectively prevented and treated, and the number of intestinal probiotics can be increased or maintained, so that the growth of pathogenic bacteria is inhibited, the dosage of antibiotics is reduced, and the aim of preventing disease infection of animals and humans can be achieved.

The invention discloses a lactobacillus strain and application thereof. The lactobacillus CGMCC6495 is separated from pickled vegetable wastewater and stored in a common microorganism center of China Committee for Culture Collection of Microorganisms with the collection date of September 3, 2012 and the collection number of CGMCC No.6495. The strain can be applied to biological deodorization of excrement through the conventional activation culture and enlargement fermentation cultivation; the lactobacillus CGMCC 6495 can be used as a single strain for in-situ deodorization of the human excrement, so that the deodorization effect is stable and the temperature influence is small. Under the normal-temperature condition, the lactobacillus strain has a better deodorization effect in both a laboratory environment and a public toilet site environment.

The invention provides a method for preparing a high-activity probiotic preparation for livestock breeding. Five bacterial-propagation pure cultures including bacillus subtilis 35-45%, bifidobacterium 15-25%, bear yeast 20-30%, lactobacilli 3-9% and photosynthetic bacteria 6-12% by mass are mixed into a bacterial mixed liquid last two hours before fermentation. Rice bran 30-38%, wheat bran 25-32%, soya bean meal 6-10%, molasses 2-5%, water 23-29%, saccharifying enzyme 0.07-0.13% and bacterial mixed liquid 0.8-1.2% by mass are evenly paved in an independently-closed fermenting tank adjustable in temperature. The method for preparing the high-activity probiotic preparation for the livestock breeding creates a three-phase bacterial-propagation technology including activation, liquid propagation and solid fermentation production. The total amount of viable bacteria is increased to the most extent, the stability of a probiotic preparation product is improved, and the probiotic preparation product can be preserved for 9-12 months under the normal-temperature condition.

The present invention relates to probiotic compositions and kits thereof, comprising live bacteria belonging to the natural flora of the human body cavity such as intestine and vaginal tract, in particular, strains of lactobacillus or bifidobacterium species, and low molecular-weight non-proteinaceous iron chelators capable of lowering the iron concentration over the whole physiological pH-range of relevance to levels that inhibit growth of pathogens, but which allow for the growth of the bacteria of the composition.

The invention provides a bacillus-subtilis-contained artificial milk extract premix for pigs and application of the premix. According to the prescription, the premix comprises the following components in percentage by weight; 30%-60% of carrier, 1%-4% of bacillus subtilis, 1%-4% of lactobacillus acidophilus, 3%-10% of coated zinc oxide, 1%-2% of chitosan oligosaccharide, 1%-2% of mannan oligosaccharide, 1%-3% of compound enzyme preparation, 10%-30% of lysine, 10%-40% of methionine, and 1%-5% of sweetener. The invention further provides the application of the bacillus subtilis-contained artificial milk extract premix. The bacillus-subtilis-contained artificial milk extract premix can be used for improving the immunity of piglets, enhancing the feed digestibility, emarkably decreasing the diarrhea rate of weaned piglets, and improving the physical conditions of the piglets, thus greatly improving the production performance of the weaned piglets.

A composition for alleviation of alcohol induced hangover comprises Ssangwhatang including Hoveniae Semen or lactobacillus fermented Ssangwhatang as an effective ingredient. Specifically, the composition comprises Ssangwhatang including Hoveniae Semen extracts or lactobacillus fermented Ssangwhatang prepared by inoculating, culturing and fermenting or lactobacillus. The composition is useful in alleviation of alcohol induced hangover and reducing blood-alcohol concentration during alcohol intake when taken as a health food.

The invention relates to a Lactobacillus planetarium strain LP28, which is preserved in China General Microbiological Culture Collection Center (CGMCC). The preservation number of the Lactobacillus planetarium strain LP28 is CGMCC No.3346. The Lactobacillus planetarium strain or metabolite thereof has excellent capability of mitigating an anaphylactic reaction and can be widely used for preparing food compounds or medical pharmaceutical compounds.

The invention relates to the fields of genetic engineering and biomedicine, and more specifically relates to a biomarker of schizophrenia and its application. The invention discloses a marker of schizophrenia and its application in preparing a kit for diagnosing schizophrenia, and also discloses a kit for detecting the marker. The schizophrenia markers disclosed in the present invention include Prevotella, Succinivibrio, Fusobacterium, Lactobacillus, and Megasphaera , Acidaminococcus, Citrobacter, Phascolarctobacterium, Coprococcus, Roseburia, Blautia 11 species At least 5 of the microorganisms. The marker provided by the invention can judge schizophrenia and normal people, the AUC is above 0.81, has good diagnostic efficiency, and can be used to prepare a kit for effectively diagnosing schizophrenia.