Micro-ecology fermentation protein feedstuff and preparation method thereof
A fermented protein feed and micro-ecological technology, applied in the field of micro-ecological fermented protein feed and its preparation, can solve the problems of many residual anti-nutritional factors, lack of bioactive peptides, residual chemical reagents, etc., to improve animal production performance, improve Immunity of the body, effect of promoting food intake
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Embodiment 1
[0025] 1. Preparation of koji for strains
[0026] (1) Bacillus subtilis (Bacillus subtilis ACCC01185, provided by China Agricultural Microorganism Culture Collection Management Center):
[0027] The slant medium is: peptone 1g, beef extract 0.3g, NaCl 0.5g, agar 2.0g, distilled water 100ml, pH 7.0. Inoculate Bacillus subtilis ACCC01185 and culture at 37°C for 24 hours to obtain slant strains;
[0028] The liquid seed medium is: glucose 0.2g, NaCl 0.5g, yeast extract 0.5g, peptone 1g, distilled water 100mL, pH7.0. Liquid seed culture: Each 300ml triangular bottle contains 50ml liquid seed medium, sterilized at 120°C for 30 minutes, after cooling down, connect a ring of bacterial lawn from the inclined surface to the seed medium, and place it on a temperature-controlled shaker at 37°C for cultivation, with a rotation speed of 220r / min , cultivated for 24 hours, the density of live bacteria ≥ 2.5×10 10 cfu / mL is the seed liquid of Bacillus subtilis.
[0029] (2) Rhizopus oli...
Embodiment 2
[0051] 1. Preparation of koji for strains
[0052] (1) Bacillus subtilis (Bacillus subtilis ACCC01185, provided by China Agricultural Microorganism Culture Collection Management Center):
[0053] The slant medium is: peptone 1g, beef extract 0.3g, NaCl 0.5g, agar 2.0g, distilled water 100ml, pH 7.0. Inoculate Bacillus subtilis ACCC01185 and culture at 37°C for 24 hours to obtain slant strains;
[0054] The liquid seed medium is: glucose 0.2g, NaCl 0.5g, yeast extract 0.5g, peptone 1g, distilled water 100mL, pH7.0. Liquid seed culture: Each 300ml triangular bottle contains 50ml liquid seed medium, sterilized at 120°C for 30 minutes, after cooling down, connect a ring of bacterial lawn from the inclined surface to the seed medium, and place it on a temperature-controlled shaker at 37°C for cultivation, with a rotation speed of 220r / min , stop when the spore rate reaches more than 90% (about 24 hours), it is the Bacillus subtilis seed liquid.
[0055] (2) Rhizopus oligosporus ...
Embodiment 3
[0077] 1. Preparation of koji for strains
[0078] (1) Bacillus subtilis (Bacillus subtilis ACCC01185, provided by China Agricultural Microorganism Culture Collection Management Center):
[0079] The slant medium is: peptone 1g, beef extract 0.3g, NaCl 0.5g, agar 2.0g, distilled water 100ml, pH 7.0. Inoculate Bacillus subtilis ACCC01185 and culture at 37°C for 24 hours to obtain slant strains;
[0080] The liquid seed medium is: glucose 0.2%, NaCl 0.5%, yeast extract 0.5%, peptone 1%, pH 7.0. Liquid seed culture: Each 300ml triangular bottle contains 50ml liquid seed medium, sterilized at 120°C for 30 minutes, after cooling down, connect a ring of bacterial lawn from the inclined surface to the seed medium, and place it on a temperature-controlled shaker at 37°C for cultivation, with a rotation speed of 220r / min , stop when the spore rate reaches more than 90% (about 24 hours), it is the Bacillus subtilis seed liquid.
[0081] (2) Rhizopus oligosporus (Rhizopus oligosporus ...
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