Micro-ecology fermentation protein feedstuff and preparation method thereof

A fermented protein feed and micro-ecological technology, applied in the field of micro-ecological fermented protein feed and its preparation, can solve the problems of many residual anti-nutritional factors, lack of bioactive peptides, residual chemical reagents, etc., to improve animal production performance, improve Immunity of the body, effect of promoting food intake

Inactive Publication Date: 2009-06-10
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many people have done a lot of work in this regard, such as using physical methods, chemical methods, biological breeding methods, etc. to eliminate or reduce the anti-nutritional factors in soybean meal. Although these methods have certain effects, they ...

Method used

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  • Micro-ecology fermentation protein feedstuff and preparation method thereof
  • Micro-ecology fermentation protein feedstuff and preparation method thereof
  • Micro-ecology fermentation protein feedstuff and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Preparation of koji for strains

[0026] (1) Bacillus subtilis (Bacillus subtilis ACCC01185, provided by China Agricultural Microorganism Culture Collection Management Center):

[0027] The slant medium is: peptone 1g, beef extract 0.3g, NaCl 0.5g, agar 2.0g, distilled water 100ml, pH 7.0. Inoculate Bacillus subtilis ACCC01185 and culture at 37°C for 24 hours to obtain slant strains;

[0028] The liquid seed medium is: glucose 0.2g, NaCl 0.5g, yeast extract 0.5g, peptone 1g, distilled water 100mL, pH7.0. Liquid seed culture: Each 300ml triangular bottle contains 50ml liquid seed medium, sterilized at 120°C for 30 minutes, after cooling down, connect a ring of bacterial lawn from the inclined surface to the seed medium, and place it on a temperature-controlled shaker at 37°C for cultivation, with a rotation speed of 220r / min , cultivated for 24 hours, the density of live bacteria ≥ 2.5×10 10 cfu / mL is the seed liquid of Bacillus subtilis.

[0029] (2) Rhizopus oli...

Embodiment 2

[0051] 1. Preparation of koji for strains

[0052] (1) Bacillus subtilis (Bacillus subtilis ACCC01185, provided by China Agricultural Microorganism Culture Collection Management Center):

[0053] The slant medium is: peptone 1g, beef extract 0.3g, NaCl 0.5g, agar 2.0g, distilled water 100ml, pH 7.0. Inoculate Bacillus subtilis ACCC01185 and culture at 37°C for 24 hours to obtain slant strains;

[0054] The liquid seed medium is: glucose 0.2g, NaCl 0.5g, yeast extract 0.5g, peptone 1g, distilled water 100mL, pH7.0. Liquid seed culture: Each 300ml triangular bottle contains 50ml liquid seed medium, sterilized at 120°C for 30 minutes, after cooling down, connect a ring of bacterial lawn from the inclined surface to the seed medium, and place it on a temperature-controlled shaker at 37°C for cultivation, with a rotation speed of 220r / min , stop when the spore rate reaches more than 90% (about 24 hours), it is the Bacillus subtilis seed liquid.

[0055] (2) Rhizopus oligosporus ...

Embodiment 3

[0077] 1. Preparation of koji for strains

[0078] (1) Bacillus subtilis (Bacillus subtilis ACCC01185, provided by China Agricultural Microorganism Culture Collection Management Center):

[0079] The slant medium is: peptone 1g, beef extract 0.3g, NaCl 0.5g, agar 2.0g, distilled water 100ml, pH 7.0. Inoculate Bacillus subtilis ACCC01185 and culture at 37°C for 24 hours to obtain slant strains;

[0080] The liquid seed medium is: glucose 0.2%, NaCl 0.5%, yeast extract 0.5%, peptone 1%, pH 7.0. Liquid seed culture: Each 300ml triangular bottle contains 50ml liquid seed medium, sterilized at 120°C for 30 minutes, after cooling down, connect a ring of bacterial lawn from the inclined surface to the seed medium, and place it on a temperature-controlled shaker at 37°C for cultivation, with a rotation speed of 220r / min , stop when the spore rate reaches more than 90% (about 24 hours), it is the Bacillus subtilis seed liquid.

[0081] (2) Rhizopus oligosporus (Rhizopus oligosporus ...

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Abstract

The invention provides a micro-ecological fermented protein feed and a preparation method, wherein the feed is prepared by mixing bean pulp and silkworms with quality ratio 90-98:2-10, then cooking to obtain solid state fermention medium, then inoculating mixed strains of bacillus subtilis, Saccharomyces cerevisiae, lactobacillus and Rhizopusoligosporus on solid fermention medium, then co-mixing for 30-45 hours at 33-37 DEG C, drying fermentation products after completing fermenting, crushing and obtaining the micro-ecological fermented protein feed. The inventive micro-ecological fermented protein feed contains a plurality of biological active peptides, content of small peptide reach more than 22%, which is favourable for digestion and development; contains lactobacillus, microzyme, which is favourable for controlling useful microbiological population in intestinal tracts of animals; has good delicious flavor and good luring effect, advances young animals for ingestion; contains soybean isoflavone more than 0.05%, markedly increases immunity of bodies, increases producibility of animals; relieving odmour of fecal waste; fully takes place of animal protein feed of fish meal and plasma protein flour and the like.

Description

(1) Technical field [0001] The invention relates to a micro-ecological fermented protein feed and a preparation method thereof. (2) Background technology [0002] The issue of "promoting the growth of young animals" has always been the focus of attention of breeding scientific and technological workers. In order to meet the special physiological growth needs of young animals, feed formulators often add a large amount of food that is similar to the source of the mother or is easy to digest and absorb. Raw materials, such as fish meal, meat and bone meal, plasma protein powder, whey powder, etc. However, the application of these raw materials makes the price of formula feed higher, which affects the benefits of the aquaculture industry. It is also because fishmeal, as a sub-scarce resource, has been strictly limited in production in major producing countries. Banned due to safety concerns. The limited use of animal protein feed and the increasing demand for high-quality feed...

Claims

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Application Information

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IPC IPC(8): A23K1/00C12N1/20A23K10/12A23K10/20A23K10/37A23K20/147
CPCY02P60/87
Inventor 陈安国徐张贤洪奇华金妙仁杨彩梅
Owner ZHEJIANG UNIV
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