Lactic bacillus strain and its separating and breeding method and use
A technology of Lactobacillus and Lactobacillus gasseri, which is applied in the field of animal nutrition and microecology, can solve the problems of large variation, unstable product quality, and unstable effect
Examples
Embodiment 1
[0247] Select 12 healthy Du long commercial hybrid piglets weaned at 21 ± 2 days old, with a body weight of 7.65 ± 1.10 kg, randomly divide them into 2 groups, and raise them in single cages on metabolic cages. The room temperature of the pig metabolic room is controlled at 25-27°C. The 6 piglets in the treatment group drank orally liquid probiotic Lactobacillus preparations every day, and the concentrations of each strain of Lactobacillus preparations per milliliter of drinking water were: Lactobacillus gasseri: 4.0×10 4 cfu / ml; Lactobacillus reuteri: 6.0×10 4 cfu / ml; Lactobacillus acidophilus: 1.0×10 5 cfu / ml; Lactobacillus fermentum: 4.0×10 4 cfu / ml. The 6 piglets in the control group drank tap water as a control, and after 7 days, they were treated with Escherichia coli K 99 、K 88 and 987P mixed culture medium (10 8 ) oral challenge, twice a day, one day in total, the incidence of diarrhea and diarrhea index after the challenge were measured, and the protective effect...
Embodiment 2
[0249] In the experiment, 36 weaned piglets from Dudu were selected, with a body weight of 7.64±1.09kg. They were randomly divided into 2 groups according to body weight and gender, each group was a treatment, and each group had 18 piglets. Each group of piglets was randomly divided into 6 groups, each group 3 One piglet has one pen, the pen area is 2.0×2.0 meters, raised on the high-rise net, each pen is a repetition, and each treatment has three pens for barrows and three pens for sows. During the test period, the piglets were kept in a fully enclosed nursery piglet house, and the temperature in the house was kept at 24-27°C. Free access to food and water. The basal diet did not contain any antibiotics, and the piglets were immunized according to the immunization procedures for routine veterinary infectious diseases in pigs. Treatment 1 added 0.1% Lactobacillus compound preparation to the basal diet; the ratio of the four species of bacteria in the Lactobacillus compound pr...
Embodiment 3
[0251] In the experiment, 72 weaned piglets from Dudu were selected, with a body weight of 8.42±1.15kg. They were randomly divided into 3 groups according to body weight and sex, each group was a treatment, and each group had 24 piglets. Each group of piglets was randomly divided into 6 groups, each group 4 One piglet has one pen, the pen area is 2.0×2.0 meters, raised on the high-rise net, each pen is a repetition, and each treatment has three pens for barrows and three pens for sows. During the test period, the piglets were kept in a fully enclosed nursery piglet house, and the temperature in the house was kept at 24-27°C. Free access to food and water. The basal diet did not contain any antibiotics, and the piglets were immunized according to the immunization procedures for routine veterinary infectious diseases in pigs. Treatment 1 was the control group, and the piglets were fed basal rations, and the nutritional level of the basal rations was prepared according to the re...
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Abstract
Description
Claims
Application Information
- IPC
- A23K20/00; A61K35/747; C12N1/20
- Inventors
- 李德发; 黄沧海