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Lactic bacillus strain and its separating and breeding method and use

A technology of Lactobacillus and Lactobacillus gasseri, which is applied in the field of animal nutrition and microecology, can solve the problems of large variation, unstable product quality, and unstable effect

Inactive Publication Date: 2004-07-14
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the instability of the effect of prebiotic products on the market is an important reason affecting the wide application of prebiotics in the aquaculture industry. The main reasons for product instability are that prebiotic products have too wide a range of targets and product quality is unstable and variable.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0247] Select 12 healthy Du long commercial hybrid piglets weaned at 21 ± 2 days old, with a body weight of 7.65 ± 1.10 kg, randomly divide them into 2 groups, and raise them in single cages on metabolic cages. The room temperature of the pig metabolic room is controlled at 25-27°C. The 6 piglets in the treatment group drank orally liquid probiotic Lactobacillus preparations every day, and the concentrations of each strain of Lactobacillus preparations per milliliter of drinking water were: Lactobacillus gasseri: 4.0×10 4 cfu / ml; Lactobacillus reuteri: 6.0×10 4 cfu / ml; Lactobacillus acidophilus: 1.0×10 5 cfu / ml; Lactobacillus fermentum: 4.0×10 4 cfu / ml. The 6 piglets in the control group drank tap water as a control, and after 7 days, they were treated with Escherichia coli K 99 、K 88 and 987P mixed culture medium (10 8 ) oral challenge, twice a day, one day in total, the incidence of diarrhea and diarrhea index after the challenge were measured, and the protective effect...

Embodiment 2

[0249] In the experiment, 36 weaned piglets from Dudu were selected, with a body weight of 7.64±1.09kg. They were randomly divided into 2 groups according to body weight and gender, each group was a treatment, and each group had 18 piglets. Each group of piglets was randomly divided into 6 groups, each group 3 One piglet has one pen, the pen area is 2.0×2.0 meters, raised on the high-rise net, each pen is a repetition, and each treatment has three pens for barrows and three pens for sows. During the test period, the piglets were kept in a fully enclosed nursery piglet house, and the temperature in the house was kept at 24-27°C. Free access to food and water. The basal diet did not contain any antibiotics, and the piglets were immunized according to the immunization procedures for routine veterinary infectious diseases in pigs. Treatment 1 added 0.1% Lactobacillus compound preparation to the basal diet; the ratio of the four species of bacteria in the Lactobacillus compound pr...

Embodiment 3

[0251] In the experiment, 72 weaned piglets from Dudu were selected, with a body weight of 8.42±1.15kg. They were randomly divided into 3 groups according to body weight and sex, each group was a treatment, and each group had 24 piglets. Each group of piglets was randomly divided into 6 groups, each group 4 One piglet has one pen, the pen area is 2.0×2.0 meters, raised on the high-rise net, each pen is a repetition, and each treatment has three pens for barrows and three pens for sows. During the test period, the piglets were kept in a fully enclosed nursery piglet house, and the temperature in the house was kept at 24-27°C. Free access to food and water. The basal diet did not contain any antibiotics, and the piglets were immunized according to the immunization procedures for routine veterinary infectious diseases in pigs. Treatment 1 was the control group, and the piglets were fed basal rations, and the nutritional level of the basal rations was prepared according to the re...

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Abstract

The present invention relates to four kinds of lactobacilli, including Lactobacillus gasseri with preservation number of CGMCC No. 0840, Lactobacillus reuteri with preservation number of CGMCC No. 0841, Lactobacillus acidophilus with preservation number of CGMCC No. 0842 and Lactobacillus fermentum with preservation number of CGMCC No. 0843, separated from piglet intestinal tract and bred. The combination of the four kinds of lactobacilli is used in preparing the probiotics preparation for preventing and treating piglet ablactation diarrhoea and promoting growth, and the probiotic preparation is added into feed for ablactated piglet to prevent and treating diarrhoea and promote its growth.

Description

field of invention [0001] The invention belongs to the field of animal nutrition and microecology, and in particular relates to 4 kinds of lactobacillus strains with interactive effects in the digestive tract of piglets and their isolation and breeding methods and uses. The compound preparation containing the lactobacillus strains can be applied to Feed additive for weaned piglets, which can prevent diseases and promote growth of piglets. Background technique [0002] Feed additives are the core issue of feed, and pharmaceutical feed additives have been widely used since the advent of antibiotics in the early 1940s. With the increase of antibiotic varieties and the improvement of production level, the price of antibacterial drugs has dropped. In the case of increasing intensification of the aquaculture industry, adding antibacterial drugs to feed has become an effective means to improve breeding efficiency. However, antimicrobial drug resistance and drug residues in animal ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K20/00A61K35/747C12N1/20
Inventor 李德发黄沧海谯仕彦马永喜邢建军
Owner CHINA AGRI UNIV
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