Method for preparing high-activity probiotic preparation for livestock breeding

A probiotic preparation and a high-activity technology, applied in the field of preparation of probiotic preparations, can solve the problems of short storage time, low bacterial content, poor stability, etc., and achieve the effects of prolonging storage time, improving vitality and improving stability

Active Publication Date: 2013-02-06
张刘运
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problems of low actual bacteria content, poor activity, single function, poor stability and short storage time in the existing probiotic additives during large-scale and industrial production, and to provide a Preparation method of highly active probiotic preparation for animal husbandry

Method used

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Embodiment Construction

[0017] The present invention will be described in further detail below: the present embodiment is implemented on the premise of the technical solution of the present invention, and detailed implementation is provided, but the protection scope of the present invention is not limited to the following examples.

[0018] A kind of preparation method of highly active probiotic preparation for animal husbandry involved in the present embodiment, the steps are as follows:

[0019] 1. Activation: Pick up inclined planes to preserve Bacillus subtilis and Saccharomyces cerevisiae, inoculate them into the seed medium of Bacillus subtilis and Saccharomyces cerevisiae, activate at 30~37℃, shaker at 150~170rpm, for 12~24h. Pick preserved strains of Lactobacillus and inoculate them into Lactobacillus medium, and culture them statically at 30-36°C for 24-36 hours to activate. Pick preserved strains of Bifidobacteria and inoculate them into Bifidobacterium culture medium, and culture them stat...

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Abstract

The invention provides a method for preparing a high-activity probiotic preparation for livestock breeding. Five bacterial-propagation pure cultures including bacillus subtilis 35-45%, bifidobacterium 15-25%, bear yeast 20-30%, lactobacilli 3-9% and photosynthetic bacteria 6-12% by mass are mixed into a bacterial mixed liquid last two hours before fermentation. Rice bran 30-38%, wheat bran 25-32%, soya bean meal 6-10%, molasses 2-5%, water 23-29%, saccharifying enzyme 0.07-0.13% and bacterial mixed liquid 0.8-1.2% by mass are evenly paved in an independently-closed fermenting tank adjustable in temperature. The method for preparing the high-activity probiotic preparation for the livestock breeding creates a three-phase bacterial-propagation technology including activation, liquid propagation and solid fermentation production. The total amount of viable bacteria is increased to the most extent, the stability of a probiotic preparation product is improved, and the probiotic preparation product can be preserved for 9-12 months under the normal-temperature condition.

Description

technical field [0001] The invention relates to a preparation method of a highly active probiotic preparation for animal husbandry, and belongs to the technical field of preparation methods of probiotic preparations. Background technique [0002] In the 1950s, it was discovered that adding low-concentration antibiotics to feed could significantly promote the growth of livestock and poultry. Since then, the use of antibiotics as feed additives has occupied a very important position. With the deepening of people's understanding of antibiotics, the hidden disadvantages behind the huge economic benefits brought by antibiotic additives have gradually been discovered: long-term use of antibiotics will lead to bacterial resistance, and will also cause the decline in the immunity of livestock and poultry, causing livestock and poultry Poultry endogenous infection and superinfection; antibiotics will cause residues in livestock products that directly threaten human health. Misuse ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00A23K1/16C12N1/20C12N1/18C12R1/125C12R1/225C12R1/865C12R1/01
Inventor 张刘运全立群曹健滨张日阔沈广月
Owner 张刘运
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