97 results about "Streptococcus mutans" patented technology

The invention provides a probiotic composition for preventing and / or inhibiting oral pathogens as well as a derivative product, a preparation method and application of the probiotic composition, and belongs to the technical field of oral care. The probiotic composition comprises the following bacterial strains in parts by mass: 90 to 0.45 part of a lactobacillus rhamnosus bacterial strain A2114, 10 to 0.3 part of a plant lactobacillus bacterial strain A2124, 80 to 0.4 part of a pediococcus pentosaceus bacterial strain A2135, 250 to 0.25 part of a lactobacillus reuteri bacterial strain A21350, and 40 to 0.2 part of a plant lactobacillus bacterial strain A2109. The probiotic composition or the metabolite thereof can effectively inhibit oral pathogens such as streptococcus mutans, porphyromonas gingivalis, helicobacter pylori, escherichia coli and staphylococcus aureus. Therefore, the probiotic composition or the metabolite thereof shows a good application prospect in the aspects of preventing and relieving oral problems such as decayed teeth, periodontitis and ozostomia.

The invention relates to the field of microorganisms, in particular to lactobacillus plantarum TRT-01 with anti-inflammatory and bacteriostatic functions as well as a preparation method and application of the lactobacillus plantarum TRT-01, the lactobacillus plantarum TRT-01 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.32237, and the preservation date is October 17, 2024. The plant lactobacillus has broad-spectrum bacteriostatic activity on 10 pathogenic bacteria such as helicobacter pylori, streptococcus mutans and the like, and can regulate Th1 / Treg immune balance and relieve inflammatory response by inducing dendritic cells to secrete cell factors IL-10 and IL-12, inducing T cells to secrete cell factors TGF-beta and IFN-gamma and inhibiting secretion of cell factors IL-6 at the same time. Therefore, the plant lactobacillus TRT-01 disclosed by the invention can be used for preparing a composition with anti-inflammatory and bacteriostatic effects, is suitable for the fields of medicines, foods, health-care products and the like, and is particularly suitable for developing a microbial preparation with a probiotic effect.

The invention relates to the technical field of microorganisms, in particular to plant lactobacillus BR32 with functions of preventing decayed teeth and improving inflammation and application of the plant lactobacillus BR32. The plant lactobacillus BR32 with the functions of preventing decayed teeth and improving inflammation, provided by the invention, has the preservation number of CGMCC No.32836, has an inhibiting effect on the growth of oral pathogens such as streptococcus mutans, candida albicans and porphyromonas gingivalis, is high in oxidation resistance, is beneficial to continuous oral cavity stability maintenance through a synergistic effect, also has relatively high hydrophobicity and self-agglutination capability, and can be used for preparing oral cavity health-care products. The compound is low in tooth environment corrosion, high in lysozyme tolerance and good in oral colonization capacity, does not have a hemolysis phenomenon in in-vitro hemolysis culture, is sensitive to common antibiotics and has relatively high food safety. The invention further provides a microbial agent, a probiotic product, a functional food, a fermented product, a bacteriostatic agent and an oral product containing the plant lactobacillus BR32 with the functions of preventing the decayed teeth and improving the inflammation, and the effects of preventing the decayed teeth, improving the inflammation, preventing dental plaque, relieving oral ulcer and the like are achieved.

The invention provides a composition and application thereof in caries prevention. The composition comprises fluoride and polypeptide, the concentration of F <-> in the fluoride in the composition is 1.79 to 7.14 mM; the concentration of the polypeptide in the composition is 1.56-12.5 [mu] M, and the amino acid sequence of the polypeptide is as shown in SEQ ID NO: 1. The natural advantage that polypeptide is not prone to drug resistance is utilized, the risk that streptococcus mutans and other bacteria generate drug resistance is greatly reduced, the inhibition effect of fluoride on streptococcus mutans can be remarkably enhanced, and a good anti-caries effect can be achieved under the low concentration (F <-> is 1.79-7.14 mM) of fluoride. Moreover, adverse effects on probiotics in the oral cavity are avoided, and the oral micro-ecological balance can be adjusted. Therefore, the composition disclosed by the invention is suitable for preparing products for preventing caries, inhibiting streptococcus mutans and regulating oral micro-ecological balance.

The invention belongs to the technical field of biomedical treatment and biological fermentation, discloses a metagen composition for effectively inhibiting oral dental caries and application thereof, and aims to solve the problems of great side effect, weak effect and unknown mechanism of the existing dental caries prevention and treatment means. The composition is prepared by mixing a metagen 1 which is prepared by fermenting lactobacillus salivarius Postbio-YY with a metagen 2 which is prepared by fermenting lactococcus lactis Postbio-F3 and lactobacillus paracasei Postbio-P6 step by step. In the preparation process, the active ingredients are reserved by accurately controlling the fermentation pH, time and pasteurization parameters. The composition can be prepared into multiple dosage forms such as toothpaste and the like, can remarkably inhibit the growth of streptococcus mutans and destroy the thallus structure, inhibits biofilm and exopolysaccharide synthesis by down-regulating fimI, wcaJ and wcae genes, is safe and free of side effects, and provides an efficient scheme for decayed tooth prevention and treatment.

The invention relates to the field of microorganisms, and discloses lactobacillus paracasei and application thereof. The strain can degrade cholesterol and inhibit the growth and reproduction of streptococcus mutans; in addition, the strain not only has relatively strong acid production capacity, but also can ferment butter under a low-temperature condition, so that the butter with strong and harmonious flavor is obtained.

The invention belongs to the technical field of synthesis and antibiosis of nano-enzyme catalysis systems, and discloses an enzyme catalysis system with a function of targeted inhibition of growth of streptococcus mutans as well as preparation and application of the enzyme catalysis system. The enzyme catalysis system with the targeted inhibition function on the growth of the streptococcus mutans is formed by loading AuNPs and PCN-22 (Fe) on a CMD molecular chain through in-situ growth, and the mass ratio of the AuNPs to the PCN-22 (Fe) to the CMD is (0.22%-0.33%) to (12.34%-21.79%) to (77.88%-87.44%). The cascade catalytic effect of the nano-enzyme is adjusted by regulating and controlling the carboxymethyl substitution degree of CMD, so that glucose in a decayed tooth pathological environment is efficiently catalyzed in one step to generate ROS, and damage to probiotics is reduced while the inhibition efficiency of pathogenic bacteria is improved.

The invention relates to the technical field of antibacterial preparations, and discloses an application of etoposide in an antibacterial preparation and the antibacterial preparation, the antibacterial preparation is a biofilm inhibitor, and etoposide inhibits formation of a biofilm or destroys the formed biofilm by down-regulating gene expression of the bacterial biofilm, so that the antibacterial effect of etoposide is improved. The biofilm is a biofilm of streptococcus mutans ATCC700610, and the biofilm is a biofilm of streptococcus mutans The antibacterial preparation comprises an antibacterial drug, an oral care product, a medical coating and an anti-caries preparation. MIC and MBC determination tests, growth curve tests, crystal violet staining tests, bacterium living / death staining tests and qRT-PCR tests prove that etoposide can effectively down-regulate biofilm related gene expression (such as gtfB, gtfC, gtfD and the like), inhibit formation of a bacterial biofilm or destroy a formed biofilm, and achieve an antibacterial effect.

The present invention discloses the use of pantothenic acid in the preparation of anticaries preparations related to Streptococcus mutans, and anticaries preparations containing pantothenic acid. The invention belongs to the field of biomedicine technology. The anticaries preparations related to Streptococcus mutans include an acid production inhibitor for Streptococcus mutans, a growth inhibitor for Streptococcus mutans, an inhibitor of cariogenic toxins of Streptococcus mutans, and an inhibitor of interspecies competition of Streptococcus mutans. Pantothenic acid can be used to influence the acid production, growth, production of cariogenic toxins, and interspecies competition of Streptococcus mutans, thereby maintaining the balance of the oral microecology.

The invention relates to a preparation method and application of erythritol galactoside. The method specifically comprises the following steps: constructing a reaction system which takes o-nitrobenzene-beta-D-galactopyranoside as a glycosyl donor, erythritol as a receptor and recombinant beta-galactosidase Bga7 as a catalytic enzyme, so as to prepare the erythritol-beta-galactoside. Wherein the amino acid sequence of the beta-galactosidase Bga7 is as shown in SEQ ID No.2, and the nucleotide sequence of the beta-galactosidase Bga7 is as shown in SEQ ID No.1. The invention also provides an application of the erythritol-beta-galactoside in preparation of daily chemical products or medical preparations for resisting streptococcus mutans infection. The invention provides a novel beta-galactosidase Bga7 gene of a glycosidase family 2, and a novel tool enzyme is provided for synthesis of galactoside products. The erythritol-beta-galactoside prepared by the invention has good effects of preventing streptococcus mutans infection and caries, and can be used for preparing daily chemical products or medical preparations for preventing streptococcus mutans infection.

The invention discloses an antibacterial polypeptide AuGF and application of the antibacterial polypeptide AuGF in preparation of a medicine for treating and / or preventing bacterial infection. The amino acid sequence of the polypeptide is as follows: SEQ ID NO.1: GLFIKIIKIKIAKSF; the antibacterial polypeptide AuGF disclosed by the invention has broad-spectrum antibacterial activity, can effectively solve the problem of bacterial drug resistance, and has a remarkable bacteriostatic effect on various bacteria including staphylococcus aureus, streptococcus mutans, actinobacillus, methicillin-resistant staphylococcus aureus and the like; the compound provided by the invention has the advantages of simple preparation process and low cost, can play a significant role at low concentration, has low minimum inhibitory concentration on various pathogenic bacteria, has strong bacteriostatic ability, and is beneficial to reduction of side effects and improvement of drug safety; the antibacterial polypeptide AuGF can also be used as a bacteriostatic additive to be applied to articles of daily use, provides an effective bacteriostatic means for daily cleaning and skin care, and has a wide market application prospect.

The present invention relates to the field of microbial technology, and provides a Pediococcus acidilactici YYS-J2 that can degrade acrylamide and produce high levels of phenyllactic acid, and its application. Pediococcus acidilactici The preservation number of YYS-J2 is CGMCC No. 28183. This *Pediococcus lactis* YYS-J2 exhibits excellent acrylamide removal capacity, high efficiency in L-phenylalanine utilization and metabolism, high-quality phenyllactic acid production capacity, and excellent *Streptococcus mutans* antibacterial activity. It also produces superoxide dismutase (SOD) and shows good tolerance in simulated gastric and intestinal fluids. This *Pediococcus lactis* YYS-J2 can provide a new probiotic source for the development of functional products such as acrylamide removal products, antibacterial products, and SOD-producing products, and has significant application value.

The invention relates to the technical field of biological analysis and detection, and particularly discloses a streptococcus mutans detection kit based on an anchored CRISPR-Cas12a enhanced paper-based micro-fluidic chip and a detection method. The detection kit comprises an RAA solution or RAA freeze-dried powder, magnesium acetate or a magnesium acetate solution, TMB or a TMB solution, an RAA forward primer, an RAA reverse primer, a cell lysis buffer, a buffer system and a paper-based micro-fluidic chip. The detection method comprises the following steps: establishing a standard curve and detecting a sample. The detection kit is used for detecting the content of streptococcus mutans and has the characteristics of high efficiency, rapidness, specificity, sensitivity, portability and the like, and the method is simple and convenient to operate, low in detection cost and capable of being applied to on-site detection of streptococcus mutans.

The invention belongs to the technical field of traditional Chinese medicines, and relates to a method for improving the sensitivity of streptococcus mutans to a glycyrrhiza uralensis extract. According to the method, the glycyrrhiza uralensis extract and hydrogen ions jointly act on streptococcus mutans in an acid environment. Research finds that the inhibitory activity of part of the effective components of the glycyrrhiza uralensis extract on streptococcus mutans in an acid environment is remarkably enhanced. The glycyrrhiza uralensis extract and hydrogen ions have a synergistic effect, and the acidic environment can improve the sensitivity of the isostreptococcus to the glycyrrhiza uralensis extract and enhance the bactericidal effect.

An oral care composition containing a specific mixture of a single rhamnolipid and a double rhamnolipid is disclosed. The composition has antibacterial efficacy against oral microorganisms, such as Porphyromonas gingivalis, Streptococcus mutans, and Campylobacter rectum. A method of treating or preventing gingivitis in a subject is also disclosed. Also disclosed are methods of preventing microorganisms from growing into a biofilm and / or reducing the number of microorganisms present in the form of a biofilm in the oral cavity of a subject.

The invention discloses toothpaste with efficient antibacterial and whitening functions. The core component of the toothpaste is a 2D / 2D KNbO / MoSpiezoelectric heterojunction nano material and tea polyphenol composite system. The KNbO / MoSheterojunction is synthesized through a hydrothermal method, the specific surface area reaches 11.13 m / g, the average pore size is 28.81 nm, and the antibacterial rate on escherichia coli within 3 minutes of ultrasonic treatment is 70%, and the antibacterial rate on staphylococcus aureus is 60.03%. The tea polyphenol (1-5mM) is preferably ester catechin (EGCG: ECG = 1: 1), and can synergistically enhance the inhibition on oral pathogenic bacteria (such as streptococcus mutans). According to the toothpaste, active oxygen sterilization is triggered by mechanical force through a piezoelectric-chemical synergistic effect, meanwhile, pigments are degraded, and the toothpaste has the functions of non-invasive whitening and enamel protection.

A chewable tablet a first probiotic strain of bacteria that inhibits Streptococcus mutans; a second probiotic strain of bacteria that inhibits one or more of Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia (i.e., the red complex); a remineralizing agent such as hydroxyapatite with a micron-level particle size; and a sugar alcohol such as xylitol.

The invention belongs to the technical field of traditional Chinese medicines, and relates to a glycyrrhiza uralensis n-butyl alcohol extract and application of effective components thereof. The invention relates to a glycyrrhiza uralensis n-butyl alcohol extract. The effective components of the glycyrrhiza uralensis n-butyl alcohol extract are prepared from glycyrrhiza uralensis isoflavanone, glycyrrhiza stem alcohol A, licorcidine, dibutyl phthalate, glycyrrhizin I, 3 '-Dimethylallylkievitone, lupinone, glycyrrhiza flavonol, edediol, 1-methoxy phaseolin and isoderrone. The glycyrrhiza uralensis n-butyl alcohol extract is prepared from the following raw materials: glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis and glycyrrhiza uralensis, the anti-streptococcus mutans activity of the glycyrrhiza uralensis n-butyl alcohol extract provided by the invention is superior to that of an extract extracted by ethanol in the same extraction method. The method has more advantages in the aspect of preventing streptococcus mutans infection and decayed teeth, and a theoretical basis is provided for research and development of new products.

The present application relates to the technical field of caries-preventing gel, and particularly discloses a preparation method of sucrose-modified polyethylene glycol nanometer ferroferric oxide caries-preventing gel, wherein sucrose is used to modify ferroferric oxide, so that the adsorption of the material to bacteria is improved, and the killing capacity of the material to Streptococcus mutans is further improved compared with dextran material.The caries-preventing gel material has good biological safety, is not prone to drug resistance, has good acid-producing bacteria adsorption and killing effect on Streptococcus mutans; meanwhile, the material has a temperature-sensitive gel dosage form, has fluidity when stored at low temperature, forms a colloidal gel in an oral environment, can stay on the tooth surface for a long time, and effectively inhibits plaque microorganisms.

The application relates to the technical field of biological medicine, in particular to a compound with a conjugated structure containing an alpha, beta-unsaturated aldehyde and application thereof. The compound can covalently modify Sortase A enzyme of propionibacterium acnes through alpha, beta-unsaturated aldehyde, inhibit transpeptidation catalysis and adhesion of gram-positive bacteria (for example, methicillin-resistant staphylococcus aureus, streptococcus mutans and propionibacterium acnes), block anchoring of membrane proteins, inhibit growth of gram-positive bacteria or kill bacteria; conjugated groups with different electric effects and steric effects are screened to adjust the covalent modification efficiency of alpha, beta-unsaturated aldehyde and Sortase A enzyme, strengthen the activity of the compound in inhibiting Sortase A enzyme, thereby improve the antibacterial activity of the compound, and further make the compound with the conjugated structure containing the alpha, beta-unsaturated aldehyde in the application be applied to the fields of biological medicine and cosmetics as an antibacterial component.

The invention discloses an antibacterial peptide and application thereof in preventing and treating dental plaque and gingival problems, and belongs to the technical field of biology. According to the invention, two cationic antibacterial peptides LR18 and LR18-2 with amphiphilic alpha-helical structures are designed from the beginning. The two antibacterial peptides have good antibacterial and bactericidal activity on oral periodontal pathogenic bacteria (including fusobacterium nucleatum, porphyromonas gingivalis and streptococcus mutans), the minimum inhibitory concentration (MIC) is 62.5-500 [mu] g / mL, and the minimum bactericidal concentration (MBC) is 62.5-2000 [mu] g / mL. The structure is simple, the chemical synthesis difficulty is low, a high-purity product can be directly synthesized, the cell hemolytic activity is low, the safety is high, the product can be used for preparing oral antibacterial drugs and / or oral care products, and oral diseases are prevented and treated by inhibiting the growth of oral periodontal pathogenic bacteria.

The invention discloses a sugarcane polyphenol and magnolol compound raw material as well as a preparation method and application thereof. The compound raw material is prepared from the following components in percentage by mass: 30 to 60 percent of sugarcane polyphenol extract (polyphenol is more than or equal to 20 percent), 10 to 30 percent of pure magnolol (the purity is more than or equal to 98 percent), 5 to 15 percent of cosolvent, 2 to 8 percent of stabilizer and the balance of deionized water. The preparation method comprises the following steps: extracting and purifying the sugarcane polyphenol and magnolol, dissolving the auxiliary materials, mixing, and sterilizing to obtain the finished product. The compound raw material can be used for oral care products such as toothpaste, can synergistically inhibit streptococcus mutans, destroy bacterial plaque, prevent caries and protect gingiva, is safe and stable, and is suitable for long-term use.

The invention discloses a covalent organic framework for efficiently preventing and treating caries as well as a preparation method and application of the covalent organic framework. The covalent organic framework nano material prepared by the invention can be efficiently combined with the enamel surface, reduce the roughness of the enamel surface and efficiently inhibit the adhesion of streptococcus mutans, and can generate high-concentration active oxygen and strongly sterilize in combination with a photodynamic therapy, thereby efficiently preventing and treating the occurrence of decayed teeth.

The present application relates to the technical field of antibacterial peptides, in particular to IPC C07K7, more particularly to a gingival protection antibacterial peptide or its stereoisomer and a preparation method and application thereof.The present application provides a gingival protection antibacterial peptide or its stereoisomer, which comprises antibacterial peptide MOAMPV14R; the antibacterial peptide MOAMPV14R comprises a structure shown in formula 1.In the present application, a new antibacterial peptide MOAMPV14R is designed and synthesized, which can effectively inhibit the activity of Streptococcus mutans, Porphyromonas gingivalis, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Listeria, and M. maltophilia, and the MIC value is 1-8 μg / mL, the antibacterial effect is excellent, the hemolytic activity and cytotoxicity are low, and the safety is good.

The invention relates to the technical field of pharmaceutical compositions, and discloses an anti-caries composition containing taxifolin as well as a preparation method and application of the anti-caries composition containing taxifolin, the anti-caries composition containing taxifolin comprises taxifolin and sodium fluoride, and the concentration ratio of taxifolin to sodium fluoride is 1mg / mL: 700ppm. Under the concentration, taxifolin and sodium fluoride generate a mutual promotion synergistic effect, and sodium fluoride obviously enhances the bacteriostatic action of taxifolin, obviously prolongs the growth delay phase of streptococcus mutans and reduces the viable count peak value in the logarithmic phase; the inhibition rate on biofilm formation is 57.4 + / -2.1%, and the antibacterial effect is remarkably enhanced; meanwhile, taxifolin enhances the demineralization resistance of sodium fluoride, and the demineralization resistance effect of only 700ppm of sodium fluoride is obviously superior to that of 1000ppm of sodium fluoride. The method disclosed by the invention has more excellent bacteriostatic activity, higher demineralization resistance and higher biological safety, and has great popularization and application values.

The invention relates to pediococcus pentosaceus CAR6 for improving carotenoid, protein peptide and plant-based flavor and application of the pediococcus pentosaceus CAR6. The preservation number of the pediococcus pentosaceus CAR6 is CGMCC (China General Microbiological Culture Collection Center) The strain has the acid stability fermentation characteristic, can avoid the problem of over-acidification caused by over-fermentation, can efficiently synthesize short-chain fatty acids such as organic acid and butyric acid, has good agglutination and self-agglutination capacity, oxidation resistance and SOD enzyme secretion capacity, and has a remarkable bacteriostatic effect on streptococcus mutans, staphylococcus aureus and shigella sonnei. When the carrot fermentation broth is applied to carrot fermentation, earthy smell and grass smell of raw materials can be effectively removed, the sensory flavor of a fermented product is remarkably improved, meanwhile, the content of carotenoid, total protein and polypeptide is increased, and the content of glucose is reduced. The strain provides a new bacterium source for fermentation of carrots and other plant-based products, and has important application value in development of flavor fermentation products, eye protection, sugar control, protein peptide and intestinal tract conditioning type healthy nutritional food.

The application discloses a kind of self-assembled polypeptide TKH and its application, belong to the field of biological medicine technology.The amino acid sequence of polypeptide TKH is as follows:TKRQQVVGLLWHLLHHLLHGKGTKRQQVVGLLWH LLHHLLH.The composition of the application includes self-assembled polypeptide TKH, polypeptide derivative or polypeptide pharmaceutically acceptable salt, ester, and pharmaceutically acceptable adjuvant.The application further discloses the application of self-assembled polypeptide TKH, polypeptide derivative or its pharmaceutically acceptable salt or ester in preparing oral care composition for dental hard tissue remineralization, and in preparing medical material for treating or preventing oral diseases.The self-assembled polypeptide TKH of the application shows significantly enhanced self-assembly ability, liquid phase separation behavior and hydroxyapatite adsorption characteristics, not only has more excellent remineralization performance, but also can simultaneously inhibit streptococcus mutans biofilm formation and acid production activity.

The invention provides an anti-streptococcus mutans polypeptide, a nucleic acid molecule and application in preparation of an anti-streptococcus mutans drug. The amino acid sequence of the anti-streptococcus mutans polypeptide is as shown in SEQ ID NO.7, and the nucleotide sequence of the anti-streptococcus mutans nucleic acid molecule is as shown in SEQ ID NO.8. The invention further provides an application of the anti-streptococcus mutans polypeptide and the nucleic acid molecule in preparation of the anti-streptococcus mutans drug. The anti-streptococcus mutans nucleic acid molecule provided by the invention can inhibit streptococcus proliferation, so that the anti-streptococcus mutans nucleic acid molecule can be used for preparing anti-streptococcus mutans drugs.

The invention discloses toothpaste with a tooth whitening function and a preparation process thereof, and relates to the technical field of toothpaste production and processing. The shower gel comprises the following components: 50% of a humectant, 22.00% of hydrated silica, 18.00% of water, 2.91% of propylene glycol, 2.1% of a cleaning agent, 0.60% of essence, 0.60% of cellulose gum, 0.50% of sodium phytate, 0.50% of a PVM / MA copolymer, 0.50% of CI 77891, 0.36% of menthol, 0.30% of xanthan gum, 0.30% of tetrasodium pyrophosphate, 0.24% of menthane formylethylamine and 0.5% of a coloring agent. According to the toothpaste disclosed by the invention, by adding the PVM / MA copolymer, the growth of bacteria in an oral cavity can be effectively inhibited, and the adhesive power of streptococcus mutans can be remarkably reduced, so that the formation of dental plaque is reduced.