78 results about "Streptococcus mutans" patented technology

The invention provides a probiotic composition for preventing and / or inhibiting oral pathogens as well as a derivative product, a preparation method and application of the probiotic composition, and belongs to the technical field of oral care. The probiotic composition comprises the following bacterial strains in parts by mass: 90 to 0.45 part of a lactobacillus rhamnosus bacterial strain A2114, 10 to 0.3 part of a plant lactobacillus bacterial strain A2124, 80 to 0.4 part of a pediococcus pentosaceus bacterial strain A2135, 250 to 0.25 part of a lactobacillus reuteri bacterial strain A21350, and 40 to 0.2 part of a plant lactobacillus bacterial strain A2109. The probiotic composition or the metabolite thereof can effectively inhibit oral pathogens such as streptococcus mutans, porphyromonas gingivalis, helicobacter pylori, escherichia coli and staphylococcus aureus. Therefore, the probiotic composition or the metabolite thereof shows a good application prospect in the aspects of preventing and relieving oral problems such as decayed teeth, periodontitis and ozostomia.

The invention relates to the field of microorganisms, in particular to lactobacillus plantarum TRT-01 with anti-inflammatory and bacteriostatic functions as well as a preparation method and application of the lactobacillus plantarum TRT-01, the lactobacillus plantarum TRT-01 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.32237, and the preservation date is October 17, 2024. The plant lactobacillus has broad-spectrum bacteriostatic activity on 10 pathogenic bacteria such as helicobacter pylori, streptococcus mutans and the like, and can regulate Th1 / Treg immune balance and relieve inflammatory response by inducing dendritic cells to secrete cell factors IL-10 and IL-12, inducing T cells to secrete cell factors TGF-beta and IFN-gamma and inhibiting secretion of cell factors IL-6 at the same time. Therefore, the plant lactobacillus TRT-01 disclosed by the invention can be used for preparing a composition with anti-inflammatory and bacteriostatic effects, is suitable for the fields of medicines, foods, health-care products and the like, and is particularly suitable for developing a microbial preparation with a probiotic effect.

The invention belongs to the technical field of biomedical treatment and biological fermentation, discloses a metagen composition for effectively inhibiting oral dental caries and application thereof, and aims to solve the problems of great side effect, weak effect and unknown mechanism of the existing dental caries prevention and treatment means. The composition is prepared by mixing a metagen 1 which is prepared by fermenting lactobacillus salivarius Postbio-YY with a metagen 2 which is prepared by fermenting lactococcus lactis Postbio-F3 and lactobacillus paracasei Postbio-P6 step by step. In the preparation process, the active ingredients are reserved by accurately controlling the fermentation pH, time and pasteurization parameters. The composition can be prepared into multiple dosage forms such as toothpaste and the like, can remarkably inhibit the growth of streptococcus mutans and destroy the thallus structure, inhibits biofilm and exopolysaccharide synthesis by down-regulating fimI, wcaJ and wcae genes, is safe and free of side effects, and provides an efficient scheme for decayed tooth prevention and treatment.

The invention belongs to the technical field of synthesis and antibiosis of nano-enzyme catalysis systems, and discloses an enzyme catalysis system with a function of targeted inhibition of growth of streptococcus mutans as well as preparation and application of the enzyme catalysis system. The enzyme catalysis system with the targeted inhibition function on the growth of the streptococcus mutans is formed by loading AuNPs and PCN-22 (Fe) on a CMD molecular chain through in-situ growth, and the mass ratio of the AuNPs to the PCN-22 (Fe) to the CMD is (0.22%-0.33%) to (12.34%-21.79%) to (77.88%-87.44%). The cascade catalytic effect of the nano-enzyme is adjusted by regulating and controlling the carboxymethyl substitution degree of CMD, so that glucose in a decayed tooth pathological environment is efficiently catalyzed in one step to generate ROS, and damage to probiotics is reduced while the inhibition efficiency of pathogenic bacteria is improved.

The invention relates to the technical field of antibacterial preparations, and discloses an application of etoposide in an antibacterial preparation and the antibacterial preparation, the antibacterial preparation is a biofilm inhibitor, and etoposide inhibits formation of a biofilm or destroys the formed biofilm by down-regulating gene expression of the bacterial biofilm, so that the antibacterial effect of etoposide is improved. The biofilm is a biofilm of streptococcus mutans ATCC700610, and the biofilm is a biofilm of streptococcus mutans The antibacterial preparation comprises an antibacterial drug, an oral care product, a medical coating and an anti-caries preparation. MIC and MBC determination tests, growth curve tests, crystal violet staining tests, bacterium living / death staining tests and qRT-PCR tests prove that etoposide can effectively down-regulate biofilm related gene expression (such as gtfB, gtfC, gtfD and the like), inhibit formation of a bacterial biofilm or destroy a formed biofilm, and achieve an antibacterial effect.

The present invention discloses the use of pantothenic acid in the preparation of anticaries preparations related to Streptococcus mutans, and anticaries preparations containing pantothenic acid. The invention belongs to the field of biomedicine technology. The anticaries preparations related to Streptococcus mutans include an acid production inhibitor for Streptococcus mutans, a growth inhibitor for Streptococcus mutans, an inhibitor of cariogenic toxins of Streptococcus mutans, and an inhibitor of interspecies competition of Streptococcus mutans. Pantothenic acid can be used to influence the acid production, growth, production of cariogenic toxins, and interspecies competition of Streptococcus mutans, thereby maintaining the balance of the oral microecology.

The invention relates to a preparation method and application of erythritol galactoside. The method specifically comprises the following steps: constructing a reaction system which takes o-nitrobenzene-beta-D-galactopyranoside as a glycosyl donor, erythritol as a receptor and recombinant beta-galactosidase Bga7 as a catalytic enzyme, so as to prepare the erythritol-beta-galactoside. Wherein the amino acid sequence of the beta-galactosidase Bga7 is as shown in SEQ ID No.2, and the nucleotide sequence of the beta-galactosidase Bga7 is as shown in SEQ ID No.1. The invention also provides an application of the erythritol-beta-galactoside in preparation of daily chemical products or medical preparations for resisting streptococcus mutans infection. The invention provides a novel beta-galactosidase Bga7 gene of a glycosidase family 2, and a novel tool enzyme is provided for synthesis of galactoside products. The erythritol-beta-galactoside prepared by the invention has good effects of preventing streptococcus mutans infection and caries, and can be used for preparing daily chemical products or medical preparations for preventing streptococcus mutans infection.

The present invention relates to the field of microbial technology, and provides a Pediococcus acidilactici YYS-J2 that can degrade acrylamide and produce high levels of phenyllactic acid, and its application. Pediococcus acidilactici The preservation number of YYS-J2 is CGMCC No. 28183. This *Pediococcus lactis* YYS-J2 exhibits excellent acrylamide removal capacity, high efficiency in L-phenylalanine utilization and metabolism, high-quality phenyllactic acid production capacity, and excellent *Streptococcus mutans* antibacterial activity. It also produces superoxide dismutase (SOD) and shows good tolerance in simulated gastric and intestinal fluids. This *Pediococcus lactis* YYS-J2 can provide a new probiotic source for the development of functional products such as acrylamide removal products, antibacterial products, and SOD-producing products, and has significant application value.

The invention relates to the technical field of biological analysis and detection, and particularly discloses a streptococcus mutans detection kit based on an anchored CRISPR-Cas12a enhanced paper-based micro-fluidic chip and a detection method. The detection kit comprises an RAA solution or RAA freeze-dried powder, magnesium acetate or a magnesium acetate solution, TMB or a TMB solution, an RAA forward primer, an RAA reverse primer, a cell lysis buffer, a buffer system and a paper-based micro-fluidic chip. The detection method comprises the following steps: establishing a standard curve and detecting a sample. The detection kit is used for detecting the content of streptococcus mutans and has the characteristics of high efficiency, rapidness, specificity, sensitivity, portability and the like, and the method is simple and convenient to operate, low in detection cost and capable of being applied to on-site detection of streptococcus mutans.

The invention belongs to the technical field of traditional Chinese medicines, and relates to a method for improving the sensitivity of streptococcus mutans to a glycyrrhiza uralensis extract. According to the method, the glycyrrhiza uralensis extract and hydrogen ions jointly act on streptococcus mutans in an acid environment. Research finds that the inhibitory activity of part of the effective components of the glycyrrhiza uralensis extract on streptococcus mutans in an acid environment is remarkably enhanced. The glycyrrhiza uralensis extract and hydrogen ions have a synergistic effect, and the acidic environment can improve the sensitivity of the isostreptococcus to the glycyrrhiza uralensis extract and enhance the bactericidal effect.

A chewable tablet a first probiotic strain of bacteria that inhibits Streptococcus mutans; a second probiotic strain of bacteria that inhibits one or more of Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia (i.e., the red complex); a remineralizing agent such as hydroxyapatite with a micron-level particle size; and a sugar alcohol such as xylitol.

The invention belongs to the technical field of traditional Chinese medicines, and relates to a glycyrrhiza uralensis n-butyl alcohol extract and application of effective components thereof. The invention relates to a glycyrrhiza uralensis n-butyl alcohol extract. The effective components of the glycyrrhiza uralensis n-butyl alcohol extract are prepared from glycyrrhiza uralensis isoflavanone, glycyrrhiza stem alcohol A, licorcidine, dibutyl phthalate, glycyrrhizin I, 3 '-Dimethylallylkievitone, lupinone, glycyrrhiza flavonol, edediol, 1-methoxy phaseolin and isoderrone. The glycyrrhiza uralensis n-butyl alcohol extract is prepared from the following raw materials: glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis and glycyrrhiza uralensis, the anti-streptococcus mutans activity of the glycyrrhiza uralensis n-butyl alcohol extract provided by the invention is superior to that of an extract extracted by ethanol in the same extraction method. The method has more advantages in the aspect of preventing streptococcus mutans infection and decayed teeth, and a theoretical basis is provided for research and development of new products.

The present application relates to the technical field of caries-preventing gel, and particularly discloses a preparation method of sucrose-modified polyethylene glycol nanometer ferroferric oxide caries-preventing gel, wherein sucrose is used to modify ferroferric oxide, so that the adsorption of the material to bacteria is improved, and the killing capacity of the material to Streptococcus mutans is further improved compared with dextran material.The caries-preventing gel material has good biological safety, is not prone to drug resistance, has good acid-producing bacteria adsorption and killing effect on Streptococcus mutans; meanwhile, the material has a temperature-sensitive gel dosage form, has fluidity when stored at low temperature, forms a colloidal gel in an oral environment, can stay on the tooth surface for a long time, and effectively inhibits plaque microorganisms.

The application relates to the technical field of biological medicine, in particular to a compound with a conjugated structure containing an alpha, beta-unsaturated aldehyde and application thereof. The compound can covalently modify Sortase A enzyme of propionibacterium acnes through alpha, beta-unsaturated aldehyde, inhibit transpeptidation catalysis and adhesion of gram-positive bacteria (for example, methicillin-resistant staphylococcus aureus, streptococcus mutans and propionibacterium acnes), block anchoring of membrane proteins, inhibit growth of gram-positive bacteria or kill bacteria; conjugated groups with different electric effects and steric effects are screened to adjust the covalent modification efficiency of alpha, beta-unsaturated aldehyde and Sortase A enzyme, strengthen the activity of the compound in inhibiting Sortase A enzyme, thereby improve the antibacterial activity of the compound, and further make the compound with the conjugated structure containing the alpha, beta-unsaturated aldehyde in the application be applied to the fields of biological medicine and cosmetics as an antibacterial component.

The invention discloses an antibacterial peptide and application thereof in preventing and treating dental plaque and gingival problems, and belongs to the technical field of biology. According to the invention, two cationic antibacterial peptides LR18 and LR18-2 with amphiphilic alpha-helical structures are designed from the beginning. The two antibacterial peptides have good antibacterial and bactericidal activity on oral periodontal pathogenic bacteria (including fusobacterium nucleatum, porphyromonas gingivalis and streptococcus mutans), the minimum inhibitory concentration (MIC) is 62.5-500 [mu] g / mL, and the minimum bactericidal concentration (MBC) is 62.5-2000 [mu] g / mL. The structure is simple, the chemical synthesis difficulty is low, a high-purity product can be directly synthesized, the cell hemolytic activity is low, the safety is high, the product can be used for preparing oral antibacterial drugs and / or oral care products, and oral diseases are prevented and treated by inhibiting the growth of oral periodontal pathogenic bacteria.

The invention discloses a sugarcane polyphenol and magnolol compound raw material as well as a preparation method and application thereof. The compound raw material is prepared from the following components in percentage by mass: 30 to 60 percent of sugarcane polyphenol extract (polyphenol is more than or equal to 20 percent), 10 to 30 percent of pure magnolol (the purity is more than or equal to 98 percent), 5 to 15 percent of cosolvent, 2 to 8 percent of stabilizer and the balance of deionized water. The preparation method comprises the following steps: extracting and purifying the sugarcane polyphenol and magnolol, dissolving the auxiliary materials, mixing, and sterilizing to obtain the finished product. The compound raw material can be used for oral care products such as toothpaste, can synergistically inhibit streptococcus mutans, destroy bacterial plaque, prevent caries and protect gingiva, is safe and stable, and is suitable for long-term use.

The invention discloses a covalent organic framework for efficiently preventing and treating caries as well as a preparation method and application of the covalent organic framework. The covalent organic framework nano material prepared by the invention can be efficiently combined with the enamel surface, reduce the roughness of the enamel surface and efficiently inhibit the adhesion of streptococcus mutans, and can generate high-concentration active oxygen and strongly sterilize in combination with a photodynamic therapy, thereby efficiently preventing and treating the occurrence of decayed teeth.

The present application relates to the technical field of antibacterial peptides, in particular to IPC C07K7, more particularly to a gingival protection antibacterial peptide or its stereoisomer and a preparation method and application thereof.The present application provides a gingival protection antibacterial peptide or its stereoisomer, which comprises antibacterial peptide MOAMPV14R; the antibacterial peptide MOAMPV14R comprises a structure shown in formula 1.In the present application, a new antibacterial peptide MOAMPV14R is designed and synthesized, which can effectively inhibit the activity of Streptococcus mutans, Porphyromonas gingivalis, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Listeria, and M. maltophilia, and the MIC value is 1-8 μg / mL, the antibacterial effect is excellent, the hemolytic activity and cytotoxicity are low, and the safety is good.

The invention relates to the technical field of pharmaceutical compositions, and discloses an anti-caries composition containing taxifolin as well as a preparation method and application of the anti-caries composition containing taxifolin, the anti-caries composition containing taxifolin comprises taxifolin and sodium fluoride, and the concentration ratio of taxifolin to sodium fluoride is 1mg / mL: 700ppm. Under the concentration, taxifolin and sodium fluoride generate a mutual promotion synergistic effect, and sodium fluoride obviously enhances the bacteriostatic action of taxifolin, obviously prolongs the growth delay phase of streptococcus mutans and reduces the viable count peak value in the logarithmic phase; the inhibition rate on biofilm formation is 57.4 + / -2.1%, and the antibacterial effect is remarkably enhanced; meanwhile, taxifolin enhances the demineralization resistance of sodium fluoride, and the demineralization resistance effect of only 700ppm of sodium fluoride is obviously superior to that of 1000ppm of sodium fluoride. The method disclosed by the invention has more excellent bacteriostatic activity, higher demineralization resistance and higher biological safety, and has great popularization and application values.

The invention relates to pediococcus pentosaceus CAR6 for improving carotenoid, protein peptide and plant-based flavor and application of the pediococcus pentosaceus CAR6. The preservation number of the pediococcus pentosaceus CAR6 is CGMCC (China General Microbiological Culture Collection Center) The strain has the acid stability fermentation characteristic, can avoid the problem of over-acidification caused by over-fermentation, can efficiently synthesize short-chain fatty acids such as organic acid and butyric acid, has good agglutination and self-agglutination capacity, oxidation resistance and SOD enzyme secretion capacity, and has a remarkable bacteriostatic effect on streptococcus mutans, staphylococcus aureus and shigella sonnei. When the carrot fermentation broth is applied to carrot fermentation, earthy smell and grass smell of raw materials can be effectively removed, the sensory flavor of a fermented product is remarkably improved, meanwhile, the content of carotenoid, total protein and polypeptide is increased, and the content of glucose is reduced. The strain provides a new bacterium source for fermentation of carrots and other plant-based products, and has important application value in development of flavor fermentation products, eye protection, sugar control, protein peptide and intestinal tract conditioning type healthy nutritional food.

The application discloses a kind of self-assembled polypeptide TKH and its application, belong to the field of biological medicine technology.The amino acid sequence of polypeptide TKH is as follows:TKRQQVVGLLWHLLHHLLHGKGTKRQQVVGLLWH LLHHLLH.The composition of the application includes self-assembled polypeptide TKH, polypeptide derivative or polypeptide pharmaceutically acceptable salt, ester, and pharmaceutically acceptable adjuvant.The application further discloses the application of self-assembled polypeptide TKH, polypeptide derivative or its pharmaceutically acceptable salt or ester in preparing oral care composition for dental hard tissue remineralization, and in preparing medical material for treating or preventing oral diseases.The self-assembled polypeptide TKH of the application shows significantly enhanced self-assembly ability, liquid phase separation behavior and hydroxyapatite adsorption characteristics, not only has more excellent remineralization performance, but also can simultaneously inhibit streptococcus mutans biofilm formation and acid production activity.

The present application relates to a kind of red kidney bean bacteriostatic active polypeptide and its preparation method.The preparation method includes the following preparation steps: S100, raw material pretreatment: the red kidney bean dreg of extracting albumin is softened and grinded homogenization to be made into slurry by adding water;S200, hydrolysis: two kinds of neutral protease hydrolysis are added to the slurry, to obtain hydrolysate;S300, membrane separation: the hydrolysate is inactivated after enzyme, and solid-liquid separation is carried out, and the obtained filtrate is separated using the ultrafiltration membrane with the molecular weight cut-off 3000Da, and the obtained ultrafiltrate is desalted and concentrated using the nanofiltration membrane with the molecular weight cut-off 300Da, to obtain red kidney bean bacteriostatic active polypeptide concentrate.The polypeptide prepared by the method of the present application has inhibitory effect on streptococcus mutans, salmonella and staphylococcus aureus.Because these bacteria are common digestive tract pathogenic bacteria, the polypeptide can be used as a product for inhibiting harmful bacteria in digestive tract or as an external bacteriostatic product.

The invention relates to the field of microorganisms, in particular to lactobacillus paracasei MG-T209 capable of improving oral health, improving intestinal health and regulating immunity and application of the lactobacillus paracasei MG-T209. The preservation number of the lactobacillus paracasei MG-T209 is CGMCC (China General Microbiological Culture Collection Center) No.35096, the preservation time of the lactobacillus paracasei MG-T209 is 2025-07-03, and the lactobacillus paracasei MG-T209 is preserved in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms. According to the invention, the lactobacillus paracasei MG-T209 can inhibit harmful microorganisms, such as streptococcus mutans, fusobacterium nucleatum, porphyromonas gingivalis and candida albicans; the microbial agent containing the lactobacillus paracasei MG-T209 can improve oral problems such as bad breath, halitosis, gingival bleeding, tooth sensitivity, dental plaque and oral ulcer.

The present application relates to the field of microorganism, discloses a lactobacillus paracasei and application thereof.The strain can degrade cholesterols and inhibit the growth and reproduction of streptococcus mutans; in addition, the strain not only has strong acid production capacity, but also can ferment butter under low temperature conditions, and the butter obtained has rich and harmonious flavor.

A Streptococcus mutans circular RNA circcsbD and use thereof are provided. The nucleotide sequence of circcsbD is set forth in SEQ ID NO: 1. circcsbD can be used as a biomarker for caries. Use of circcsbD in the preparation of a formulation for preventing or treating oral caries is provided. The present invention further relates to a primer and kit for detecting circcsbD, and a strain of Streptococcus mutans overexpressing circcsbD, a construction method therefor, and use of the overexpression strain. The endogenous circular RNA circcsbD is found in the prokaryote Streptococcus mutans for the first time. The expression level of circcsbD can be used for predicting caries. circcsbD can be used as a caries-related biomarker and a treatment target. The strain overexpressing circcsbD has the effects of inhibiting extracellular polysaccharide metabolism on the biofilm of Streptococcus mutans and reducing the cariogenic capacity of Streptococcus mutans.

The application discloses an oral cavity bacteriostatic composition synthesized by using panax notoginseng and tea polyphenol and a preparation method thereof, and relates to the technical field of nano modification. Specifically, metal-polyphenol nano cores are formed by metal ion coordination self-assembly, gallic acid catechin gallate, a core component of tea polyphenol, is synergistically compounded with panax notoginseng saponin R1, and a core-shell structure nanoparticle is constructed by adopting a chitosan-sodium tripolyphosphate ionic gel crosslinking technology. The composition realizes multi-target bacteriostasis (MIC 15.7-22.1 μg / mL) and anti-inflammatory effect (TNF-alpha inhibition rate 75.4-84.2%) on oral cavity pathogenic bacteria (such as streptococcus mutans and porphyromonas gingivalis), simultaneously solves problems of easy oxidation and degradation of EGCG, short retention time and poor penetration of traditional preparations, and provides a green and efficient oral care solution.

The invention discloses an oral antibacterial liquid for an oral antibacterial mucoprotective film, which has an excellent inhibition effect on escherichia coli, porphyromonas gingivalis, staphylococcus aureus, streptococcus mutans and candida albicans through the synergistic effect of lysozyme, lysostaphin, angelia and plant extract. The bacteriostasis rate can reach 99.99% or above after 5 minutes of action; through a specific ratio and a specific process, after the active ingredients in the product are subjected to an accelerated test at 37 DEG C for 90 days, the activity decline rate of lysozyme and lysostaphin is lower than 10%, the color and precipitation are not changed, the validity period of the product can stably reach 2 years, and the efficacy in the shelf life is ensured; an acute eye stimulation test verifies that the product has no stimulation to New Zealand rabbit eyes, which indicates that the product is safe and mild when being used for oral mucosa, and stimulation and side effects possibly brought by a chemical bacteriostatic agent are avoided.

This invention discloses a synergistic anti-caries oral care composition and its application. The composition comprises: (a) a single-domain antibody targeting the Streptococcus mutans surface protein PAc; (b) a plant extract complex containing kudzu root extract and phellodendron bark extract; and (c) gallic acid liposomes. This invention also provides a toothpaste containing this composition and a method for its preparation. Experiments have shown that the single-domain antibody can specifically bind to and disturb the Streptococcus mutans biofilm, increasing cell membrane permeability and synergistically promoting the entry of plant extracts into the cell to exert a bactericidal effect, achieving a clearance rate of over 98% for mature biofilms. Through formulation and process optimization, this invention solves the stability problem of antibodies in toothpaste; the product retains more than 90% of its activity after two years of storage at 25°C. The composition and product provided by this invention have the advantages of highly efficient removal of mature biofilms, synergistic effect, safety with low damage, and excellent stability, and have broad application prospects in the field of oral care.

The present invention primarily relates to an oral hygiene composition comprising one or more specific essential oil(s) and / or essential oil component(s) asserting a antibacterial effect against Streptococcus mutans. The invention further relates to a use of said oral hygiene composition to assert an antibacterial effect against Streptococcus mutans and to reduce lactic acid concentration in the oral cavity. The invention also relates to a method of increasing oral hygiene. Further, the invention relates to an oral hygiene composition for use in a method of preventing, mitigating and / or treating a dental plaque-associated disease or condition.