55 results about "Streptococcus mutans" patented technology

The invention belongs to the technical field of synthesis and antibiosis of nano-enzyme catalysis systems, and discloses an enzyme catalysis system with a function of targeted inhibition of growth of streptococcus mutans as well as preparation and application of the enzyme catalysis system. The enzyme catalysis system with the targeted inhibition function on the growth of the streptococcus mutans is formed by loading AuNPs and PCN-22 (Fe) on a CMD molecular chain through in-situ growth, and the mass ratio of the AuNPs to the PCN-22 (Fe) to the CMD is (0.22%-0.33%) to (12.34%-21.79%) to (77.88%-87.44%). The cascade catalytic effect of the nano-enzyme is adjusted by regulating and controlling the carboxymethyl substitution degree of CMD, so that glucose in a decayed tooth pathological environment is efficiently catalyzed in one step to generate ROS, and damage to probiotics is reduced while the inhibition efficiency of pathogenic bacteria is improved.

The invention relates to a preparation method and application of erythritol galactoside. The method specifically comprises the following steps: constructing a reaction system which takes o-nitrobenzene-beta-D-galactopyranoside as a glycosyl donor, erythritol as a receptor and recombinant beta-galactosidase Bga7 as a catalytic enzyme, so as to prepare the erythritol-beta-galactoside. Wherein the amino acid sequence of the beta-galactosidase Bga7 is as shown in SEQ ID No.2, and the nucleotide sequence of the beta-galactosidase Bga7 is as shown in SEQ ID No.1. The invention also provides an application of the erythritol-beta-galactoside in preparation of daily chemical products or medical preparations for resisting streptococcus mutans infection. The invention provides a novel beta-galactosidase Bga7 gene of a glycosidase family 2, and a novel tool enzyme is provided for synthesis of galactoside products. The erythritol-beta-galactoside prepared by the invention has good effects of preventing streptococcus mutans infection and caries, and can be used for preparing daily chemical products or medical preparations for preventing streptococcus mutans infection.

The invention relates to the technical field of biological analysis and detection, and particularly discloses a streptococcus mutans detection kit based on an anchored CRISPR-Cas12a enhanced paper-based micro-fluidic chip and a detection method. The detection kit comprises an RAA solution or RAA freeze-dried powder, magnesium acetate or a magnesium acetate solution, TMB or a TMB solution, an RAA forward primer, an RAA reverse primer, a cell lysis buffer, a buffer system and a paper-based micro-fluidic chip. The detection method comprises the following steps: establishing a standard curve and detecting a sample. The detection kit is used for detecting the content of streptococcus mutans and has the characteristics of high efficiency, rapidness, specificity, sensitivity, portability and the like, and the method is simple and convenient to operate, low in detection cost and capable of being applied to on-site detection of streptococcus mutans.

The invention belongs to the technical field of traditional Chinese medicines, and relates to a method for improving the sensitivity of streptococcus mutans to a glycyrrhiza uralensis extract. According to the method, the glycyrrhiza uralensis extract and hydrogen ions jointly act on streptococcus mutans in an acid environment. Research finds that the inhibitory activity of part of the effective components of the glycyrrhiza uralensis extract on streptococcus mutans in an acid environment is remarkably enhanced. The glycyrrhiza uralensis extract and hydrogen ions have a synergistic effect, and the acidic environment can improve the sensitivity of the isostreptococcus to the glycyrrhiza uralensis extract and enhance the bactericidal effect.

A chewable tablet a first probiotic strain of bacteria that inhibits Streptococcus mutans; a second probiotic strain of bacteria that inhibits one or more of Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia (i.e., the red complex); a remineralizing agent such as hydroxyapatite with a micron-level particle size; and a sugar alcohol such as xylitol.

The invention belongs to the technical field of traditional Chinese medicines, and relates to a glycyrrhiza uralensis n-butyl alcohol extract and application of effective components thereof. The invention relates to a glycyrrhiza uralensis n-butyl alcohol extract. The effective components of the glycyrrhiza uralensis n-butyl alcohol extract are prepared from glycyrrhiza uralensis isoflavanone, glycyrrhiza stem alcohol A, licorcidine, dibutyl phthalate, glycyrrhizin I, 3 '-Dimethylallylkievitone, lupinone, glycyrrhiza flavonol, edediol, 1-methoxy phaseolin and isoderrone. The glycyrrhiza uralensis n-butyl alcohol extract is prepared from the following raw materials: glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis and glycyrrhiza uralensis, the anti-streptococcus mutans activity of the glycyrrhiza uralensis n-butyl alcohol extract provided by the invention is superior to that of an extract extracted by ethanol in the same extraction method. The method has more advantages in the aspect of preventing streptococcus mutans infection and decayed teeth, and a theoretical basis is provided for research and development of new products.

The application relates to the technical field of biological medicine, in particular to a compound with a conjugated structure containing an alpha, beta-unsaturated aldehyde and application thereof. The compound can covalently modify Sortase A enzyme of propionibacterium acnes through alpha, beta-unsaturated aldehyde, inhibit transpeptidation catalysis and adhesion of gram-positive bacteria (for example, methicillin-resistant staphylococcus aureus, streptococcus mutans and propionibacterium acnes), block anchoring of membrane proteins, inhibit growth of gram-positive bacteria or kill bacteria; conjugated groups with different electric effects and steric effects are screened to adjust the covalent modification efficiency of alpha, beta-unsaturated aldehyde and Sortase A enzyme, strengthen the activity of the compound in inhibiting Sortase A enzyme, thereby improve the antibacterial activity of the compound, and further make the compound with the conjugated structure containing the alpha, beta-unsaturated aldehyde in the application be applied to the fields of biological medicine and cosmetics as an antibacterial component.

The invention discloses an antibacterial peptide and application thereof in preventing and treating dental plaque and gingival problems, and belongs to the technical field of biology. According to the invention, two cationic antibacterial peptides LR18 and LR18-2 with amphiphilic alpha-helical structures are designed from the beginning. The two antibacterial peptides have good antibacterial and bactericidal activity on oral periodontal pathogenic bacteria (including fusobacterium nucleatum, porphyromonas gingivalis and streptococcus mutans), the minimum inhibitory concentration (MIC) is 62.5-500 [mu] g / mL, and the minimum bactericidal concentration (MBC) is 62.5-2000 [mu] g / mL. The structure is simple, the chemical synthesis difficulty is low, a high-purity product can be directly synthesized, the cell hemolytic activity is low, the safety is high, the product can be used for preparing oral antibacterial drugs and / or oral care products, and oral diseases are prevented and treated by inhibiting the growth of oral periodontal pathogenic bacteria.

The present application relates to the technical field of antibacterial peptides, in particular to IPC C07K7, more particularly to a gingival protection antibacterial peptide or its stereoisomer and a preparation method and application thereof.The present application provides a gingival protection antibacterial peptide or its stereoisomer, which comprises antibacterial peptide MOAMPV14R; the antibacterial peptide MOAMPV14R comprises a structure shown in formula 1.In the present application, a new antibacterial peptide MOAMPV14R is designed and synthesized, which can effectively inhibit the activity of Streptococcus mutans, Porphyromonas gingivalis, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Listeria, and M. maltophilia, and the MIC value is 1-8 μg / mL, the antibacterial effect is excellent, the hemolytic activity and cytotoxicity are low, and the safety is good.

The invention relates to the technical field of pharmaceutical compositions, and discloses an anti-caries composition containing taxifolin as well as a preparation method and application of the anti-caries composition containing taxifolin, the anti-caries composition containing taxifolin comprises taxifolin and sodium fluoride, and the concentration ratio of taxifolin to sodium fluoride is 1mg / mL: 700ppm. Under the concentration, taxifolin and sodium fluoride generate a mutual promotion synergistic effect, and sodium fluoride obviously enhances the bacteriostatic action of taxifolin, obviously prolongs the growth delay phase of streptococcus mutans and reduces the viable count peak value in the logarithmic phase; the inhibition rate on biofilm formation is 57.4 + / -2.1%, and the antibacterial effect is remarkably enhanced; meanwhile, taxifolin enhances the demineralization resistance of sodium fluoride, and the demineralization resistance effect of only 700ppm of sodium fluoride is obviously superior to that of 1000ppm of sodium fluoride. The method disclosed by the invention has more excellent bacteriostatic activity, higher demineralization resistance and higher biological safety, and has great popularization and application values.

The invention relates to pediococcus pentosaceus CAR6 for improving carotenoid, protein peptide and plant-based flavor and application of the pediococcus pentosaceus CAR6. The preservation number of the pediococcus pentosaceus CAR6 is CGMCC (China General Microbiological Culture Collection Center) The strain has the acid stability fermentation characteristic, can avoid the problem of over-acidification caused by over-fermentation, can efficiently synthesize short-chain fatty acids such as organic acid and butyric acid, has good agglutination and self-agglutination capacity, oxidation resistance and SOD enzyme secretion capacity, and has a remarkable bacteriostatic effect on streptococcus mutans, staphylococcus aureus and shigella sonnei. When the carrot fermentation broth is applied to carrot fermentation, earthy smell and grass smell of raw materials can be effectively removed, the sensory flavor of a fermented product is remarkably improved, meanwhile, the content of carotenoid, total protein and polypeptide is increased, and the content of glucose is reduced. The strain provides a new bacterium source for fermentation of carrots and other plant-based products, and has important application value in development of flavor fermentation products, eye protection, sugar control, protein peptide and intestinal tract conditioning type healthy nutritional food.

The application discloses a kind of self-assembled polypeptide TKH and its application, belong to the field of biological medicine technology.The amino acid sequence of polypeptide TKH is as follows:TKRQQVVGLLWHLLHHLLHGKGTKRQQVVGLLWH LLHHLLH.The composition of the application includes self-assembled polypeptide TKH, polypeptide derivative or polypeptide pharmaceutically acceptable salt, ester, and pharmaceutically acceptable adjuvant.The application further discloses the application of self-assembled polypeptide TKH, polypeptide derivative or its pharmaceutically acceptable salt or ester in preparing oral care composition for dental hard tissue remineralization, and in preparing medical material for treating or preventing oral diseases.The self-assembled polypeptide TKH of the application shows significantly enhanced self-assembly ability, liquid phase separation behavior and hydroxyapatite adsorption characteristics, not only has more excellent remineralization performance, but also can simultaneously inhibit streptococcus mutans biofilm formation and acid production activity.

The present application relates to a kind of red kidney bean bacteriostatic active polypeptide and its preparation method.The preparation method includes the following preparation steps: S100, raw material pretreatment: the red kidney bean dreg of extracting albumin is softened and grinded homogenization to be made into slurry by adding water;S200, hydrolysis: two kinds of neutral protease hydrolysis are added to the slurry, to obtain hydrolysate;S300, membrane separation: the hydrolysate is inactivated after enzyme, and solid-liquid separation is carried out, and the obtained filtrate is separated using the ultrafiltration membrane with the molecular weight cut-off 3000Da, and the obtained ultrafiltrate is desalted and concentrated using the nanofiltration membrane with the molecular weight cut-off 300Da, to obtain red kidney bean bacteriostatic active polypeptide concentrate.The polypeptide prepared by the method of the present application has inhibitory effect on streptococcus mutans, salmonella and staphylococcus aureus.Because these bacteria are common digestive tract pathogenic bacteria, the polypeptide can be used as a product for inhibiting harmful bacteria in digestive tract or as an external bacteriostatic product.

The invention relates to the field of microorganisms, in particular to lactobacillus paracasei MG-T209 capable of improving oral health, improving intestinal health and regulating immunity and application of the lactobacillus paracasei MG-T209. The preservation number of the lactobacillus paracasei MG-T209 is CGMCC (China General Microbiological Culture Collection Center) No.35096, the preservation time of the lactobacillus paracasei MG-T209 is 2025-07-03, and the lactobacillus paracasei MG-T209 is preserved in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms. According to the invention, the lactobacillus paracasei MG-T209 can inhibit harmful microorganisms, such as streptococcus mutans, fusobacterium nucleatum, porphyromonas gingivalis and candida albicans; the microbial agent containing the lactobacillus paracasei MG-T209 can improve oral problems such as bad breath, halitosis, gingival bleeding, tooth sensitivity, dental plaque and oral ulcer.

The application discloses an oral cavity bacteriostatic composition synthesized by using panax notoginseng and tea polyphenol and a preparation method thereof, and relates to the technical field of nano modification. Specifically, metal-polyphenol nano cores are formed by metal ion coordination self-assembly, gallic acid catechin gallate, a core component of tea polyphenol, is synergistically compounded with panax notoginseng saponin R1, and a core-shell structure nanoparticle is constructed by adopting a chitosan-sodium tripolyphosphate ionic gel crosslinking technology. The composition realizes multi-target bacteriostasis (MIC 15.7-22.1 μg / mL) and anti-inflammatory effect (TNF-alpha inhibition rate 75.4-84.2%) on oral cavity pathogenic bacteria (such as streptococcus mutans and porphyromonas gingivalis), simultaneously solves problems of easy oxidation and degradation of EGCG, short retention time and poor penetration of traditional preparations, and provides a green and efficient oral care solution.

The invention discloses an oral antibacterial liquid for an oral antibacterial mucoprotective film, which has an excellent inhibition effect on escherichia coli, porphyromonas gingivalis, staphylococcus aureus, streptococcus mutans and candida albicans through the synergistic effect of lysozyme, lysostaphin, angelia and plant extract. The bacteriostasis rate can reach 99.99% or above after 5 minutes of action; through a specific ratio and a specific process, after the active ingredients in the product are subjected to an accelerated test at 37 DEG C for 90 days, the activity decline rate of lysozyme and lysostaphin is lower than 10%, the color and precipitation are not changed, the validity period of the product can stably reach 2 years, and the efficacy in the shelf life is ensured; an acute eye stimulation test verifies that the product has no stimulation to New Zealand rabbit eyes, which indicates that the product is safe and mild when being used for oral mucosa, and stimulation and side effects possibly brought by a chemical bacteriostatic agent are avoided.

This invention discloses a synergistic anti-caries oral care composition and its application. The composition comprises: (a) a single-domain antibody targeting the Streptococcus mutans surface protein PAc; (b) a plant extract complex containing kudzu root extract and phellodendron bark extract; and (c) gallic acid liposomes. This invention also provides a toothpaste containing this composition and a method for its preparation. Experiments have shown that the single-domain antibody can specifically bind to and disturb the Streptococcus mutans biofilm, increasing cell membrane permeability and synergistically promoting the entry of plant extracts into the cell to exert a bactericidal effect, achieving a clearance rate of over 98% for mature biofilms. Through formulation and process optimization, this invention solves the stability problem of antibodies in toothpaste; the product retains more than 90% of its activity after two years of storage at 25°C. The composition and product provided by this invention have the advantages of highly efficient removal of mature biofilms, synergistic effect, safety with low damage, and excellent stability, and have broad application prospects in the field of oral care.

The present invention primarily relates to an oral hygiene composition comprising one or more specific essential oil(s) and / or essential oil component(s) asserting a antibacterial effect against Streptococcus mutans. The invention further relates to a use of said oral hygiene composition to assert an antibacterial effect against Streptococcus mutans and to reduce lactic acid concentration in the oral cavity. The invention also relates to a method of increasing oral hygiene. Further, the invention relates to an oral hygiene composition for use in a method of preventing, mitigating and / or treating a dental plaque-associated disease or condition.

The application relates to the application of natural plant extracts in the field of oral health, and particularly discloses the application of an extract of Ilex hainanensis Merr in oral health care products. The Ilex hainanensis Merr dichloromethane extract is eluted by high-concentration ethanol through a macroporous resin, and is rich in dihydrochalcone active ingredients, has significant inhibitory activity on Porphyromonas gingivalis and Fusobacterium nucleatum, and has no inhibitory effect on Streptococcus mutans, can selectively inhibit periodontal pathogenic bacteria without destroying the oral microecological balance, and is suitable for preparing toothpaste, mouthwash, oral spray and other oral health care products.

This invention discloses the use of phytic acid in the preparation of drugs or functional foods for regulating intestinal flora imbalance caused by Streptococcus mutans, belonging to the field of biomedicine and microecological regulation technology. This invention is the first to discover that oral phytic acid can effectively inhibit the abnormal colonization of Streptococcus mutans in the intestine, restore intestinal flora diversity, increase the abundance of beneficial bacteria (such as Bifidobacterium and Lactobacillus), reduce the proportion of pro-inflammatory bacteria, and improve intestinal barrier function and inflammatory status. This invention provides a new strategy for regulating intestinal flora imbalance caused by Streptococcus mutans.

This application relates to the field of biotechnology and discloses a protein construct, a polynucleotide, and a recombinant host cell comprising a polypeptide having 1,3-α-glucanase activity. The polypeptide can be used in oral care products, including a polypeptide with the sequence shown in SEQ ID NO:3; and at least one oral care ingredient; wherein the polypeptide has activity in inhibiting and / or clearing biofilm formation of *Streptococcus mutans*. This application provides a polypeptide that inhibits the formation of *Streptococcus mutans* biofilm in the oral cavity, and an enzyme variant with biofilm-clearing effects, the enzyme variant providing stronger biofilm-clearing ability and exhibiting enzyme activity at oral pH levels.

The present application relates to: a Corynebacterium genus microorganism having an L-amino acid producing ability, into which an NAD-dependent isocitrate dehydrogenase protein derived from Streptococcus mutans or a polynucleotide encoding the same is introduced; a method for producing an L-amino acid, the method comprising a step of culturing the microorganism in a culture medium; a composition for producing an L-amino acid, the composition comprising the microorganism, a culture of the microorganism, a fermentation product of the microorganism, or a combination of two or more thereof; and to the use of said microorganism in the production of L-amino acids.

To provide a composition capable of exhibiting new effects of bay laurel (LaurusnobilisL.).SOLUTION: The composition contains an extract of bay laurel (LaurusnobilisL.), has an antibacterial action against Tannerellaforsythensis and Streptococcusmutans, suppresses gingival inflammation, spontaneous bleeding of gums and plaque accumulation, improves dryness in the oral cavity and saliva pH, suppresses halitosis and improves constipation. Specifically, the present invention relates to an antibacterial agent against periodontal disease-related bacteria and dental caries-related bacteria, a composition for preventing or treating periodontal disease, a composition for preventing or treating dental caries, a composition for improving gingiva, a composition for improving gums, a composition for suppressing plaque (dental plaque) accumulation, a composition for improving xerostomia, a composition for improving saliva, a composition for suppressing halitosis, and a composition for improving constipation, each of which contains a bay laurel (LaurusnobilisL.) extract as an active ingredient. By containing the bay laurel (LaurusnobilisL.) extract, it is possible to suppress Tannerellaforsythensis and Streptococcusmutans which are periodontal disease-related bacteria, suppress gingival inflammation, spontaneous bleeding of gums, and plaque accumulation, improve dryness in the oral cavity and saliva pH, suppress halitosis, and improve constipation.SELECTED DRAWING: None

This invention belongs to the interdisciplinary field of natural product medicinal chemistry and oral medicine, specifically disclosing the application of uvangoletin in the preparation of anti-periodontalgia drugs. Uvangoletin is 1-(2,4-dihydroxy-6-methoxyphenyl)-3-phenyl-1-propanone, with the molecular formula C. 16 H 16 O4, experiments confirmed that its MIC for Porphyromonas gingivalis was 16 μmol / L, MIC 50 The concentration is 10.28 μmol / L, the MIC for Fusobacterium nucleatum is 16 μmol / L and the MIC50 is 6.86 μmol / L. It has no inhibitory activity against Streptococcus mutans and has selective anti-periodontal pathogenic bacteria characteristics. It can be made into oral preparations such as gels, mouthwashes or toothpaste.

This invention discloses the application of L-tryptophan in the preparation of anti-caries oral care products, belonging to the field of biomedical technology. Addressing the safety issues, oral microecological disruption, and susceptibility to drug resistance associated with existing anti-caries agents (such as fluoride and chlorhexidine), this invention proposes using L-tryptophan as the effective active ingredient in anti-caries agents. Utilizing the specific effects of L-tryptophan on *Streptococcus mutans*, it achieves the effects of inhibiting tooth decay, maintaining oral microecological balance, and reducing the risk of bacterial drug resistance. This specific effect, without affecting the survival of *Streptococcus mutans*, simultaneously inhibits the synthesis of water-insoluble extracellular polysaccharides, inhibits acid production and acid tolerance, and inhibits oxygen tolerance and interspecies competition. It is characterized by its clear efficacy and high safety.

The application discloses a traditional Chinese medicine composition of paris polyphylla and application of the traditional Chinese medicine composition in preparation of an oral cavity pathogenic bacterium activity inhibitor. The traditional Chinese medicine extract and extract of the application have the advantages of being natural, efficient, inexpensive, low in toxicity, easy to obtain and the like, and have obvious inhibiting effects on formation of oral cavity pathogenic bacterium Streptococcus mutans biofilm. The biofilm inhibition rate of the paris polyphylla alcohol extract is above 90%, the bacteriostatic rate of the Coptis deltoidea water extract is above 90%, and the bacteriostatic rate and the biofilm inhibition rate of the combination of the two are above 99%. The active ingredient acquisition method of the application is simple, safe and low in cost, and is convenient to popularize and use. The application further provides a natural medicine combination caries prevention method. The combination of drugs can not only enhance the treatment effect, but also serve as an effective prevention means to reduce drug resistance of oral cavity pathogenic bacteria.

There is currently a high demand for new therapeutic antibiotics with new mechanisms of action. Disclosed are two small molecules, tryglysin A and B, with novel chemical structures and potent antibiotic activity against a narrow spectrum of bacteria. This narrow spectrum of activity indicates the tryglysins could be working through a novel antibiotic mechanism of action. Due to this narrow spectrum, the tryglysins could be used as highly targeted therapeutics to treat or prevent disease without disturbing other important, “beneficial” bacteria within the human microbiome, which is a great improvement over virtually all other clinically used antibiotics that are broad-spectrum. The tryglysins are potent against several streptococcal pathogens, including Streptococcus pneumonia, the leading cause of pneumonia, and Streptococcus mutans, the primary causative agent of tooth decay and gum disease. The tryglycins may allow the development of therapeutics for the treatment and / or prevention of disease caused by these streptococci.

The invention discloses a treatment method of oral medical combined pollution sludge. According to the method, deep dehydration of sludge, efficient inactivation of pathogens and stabilization of heavy metals are realized through the synergistic effect of a multi-frequency gradient ultrasonic system and a response type nano-catalyst. Aiming at specific pathogenic bacteria such as porphyromonas gingivalis, streptococcus mutans and the like and heavy metals such as mercury, silver and the like in the oral sludge, the method has the advantages of thorough treatment, low energy consumption and sludge cake water content of less than 50%, and is suitable for harmless and resourceful treatment of sludge in scenes such as oral hospitals and dental outpatient clinics.

Provided is a method of administering a composition having antibacterial effects against Tannerella forsythensis and Streptococcus mutans, inhibiting effects on gum inflammation, spontaneous bleeding of gums and plaque accumulation, improving effects on xerostomia and salivary pH, inhibiting effects on bad breath, and improving effects on constipation. The composition contains bay tree (Laurus nobilis L.) extract.