The invention is applicable to the technical field of 
oral cavity health-care articles and provides 
toothpaste and a preparation method thereof. The 
toothpaste is prepared from the following components in percentage by weight: 3 percent to 5 percent of an anionic surfactant, 0.5 percent to 1.5 percent of a compound 
enzyme preparation, 30 percent to 35 percent of a 
grinding agent, 35 percent to 45percent of a 
humectant, 0.25 percent to 0.9 percent of a thickener, 0.2 percent to 0.5 percent of a 
preservative, 0.1 percent to 0.2 percent of a pH (Potential of 
Hydrogen) 
regulator and the balance of de-ionized water, wherein the compound 
enzyme preparation is a mixture of at least five of 
glucanase, 
glucose oxidase, 
polyphenol oxidase, 
lysozyme, coenzyme Q10, 
amylase, pancreatin, 
catalase, bromelain and proteinase. The 
toothpaste provided by the invention does not contain 
fluorine and adopts food-grade raw materials, so that the product has high safety, is 
environmentally friendly and easyto degrade and has no 
toxin and no residues; the 
enzyme activity stability of the compound enzyme preparation in a formula 
system is high; the toothpaste can be used for effectively enhancing the decayed tooth prevention capability, also can be used for effectively alleviating the gingival bleeding symptom; meanwhile, the toothpaste has the effects of sterilizing and diminishing 
inflammation, whitening teeth and refreshing breath.