The invention provides a preparation method of an environment-friendly enzyme detergent for down feather, comprising the following steps: (1), selecting plants, fruit peels and fresh kitchen waste functional in washing and deterging; (2), burdening according to a ratio of water, the plants, fruit peels and fresh kitchen, and brown sugar being 10:3:1; (3), placing in a container, and stirring well; (4), sealing, opening and stirring once a day every 1-30 days, and sealing and storing for longer than 60 days; (5), filtering to obtain stock solution; (6), adding plants effective in deterging into the stock solution; (7), sealing and soaking for 10-20 days; (8), removing residue, and filtering. The invention has the advantages that the detergent shows better deterging power in washing down feather, is effective in removing various bacteria and viruses and longer in antibacterial time, and rarely grows germs in long-term usage; treatment work for discharged wastewater is greatly lower than that of common chemical detergents; the detergent is made from fruit peels, plants and kitchen waste, waste is turned into wealth, and the environmental pollution due to discarded fruit peels, plants and kitchen waste is also reduced.