Preparing method of natural bamboo leaf extract antimicrobial agent

Abstract

The present invention relates to a preparing method of a natural bamboo leaf extract antimicrobial agent, belonging to the field of preparing antibacterial agents. The method uses fresh moso bamboo leaves as a raw material. After yellow and decaying bamboo leaves are removed, the moso bamboo leaves are dried, the dried moso bamboo leaves are pulverized, the moso bamboo leaf powder is dissolved in a phosphate buffer, and the mixture is subjected to magnetic stirring in an ice-water bath to obtain a bamboo leaf pulp. Then the bamboo leaf pulp is centrifuged at a high-speed to obtain a supernatant. Ammonium sulfate is used to salt out crude antimicrobial protein peptide, and finally a natural bamboo leaf extract antimicrobial agent is obtained by dialysis. The used raw materials are natural plant leaves which are renewable resources and are large in resource maintaining amount and low in production cost, and so that the antimicrobial agent is easy in preparation steps and operations. The bamboo leaves contain a variety of natural compounds. The natural bamboo leaf extract antimicrobial agent has a good antibacterial effect, produces no influence on the flavor of food to be preserved, and has an antibacterial effect significantly higher than that of ordinary chemical antibacterial agents.

Description

technical field [0001] The invention relates to a preparation method of a natural bamboo leaf extract antibacterial agent, belonging to the field of antibacterial agent preparation. Background technique [0002] Generally, the preservatives used in food include synthetic preservatives and natural preservatives extracted from biological raw materials. In the past, synthetic preservatives were widely used because of their low cost, convenient use, and remarkable effect. However, with the advancement of science and technology, many synthetic chemical preservatives have increasingly exposed potential safety problems and adverse effects on the ecological environment. Therefore, there is an urgent need for people to discover and study some natural preservatives with good antiseptic effects. This preservative not only requires the ability to inhibit the contamination and reproduction of spoilage microorganisms, but also is harmless to human health, and cannot destroy the local fla...

AI Technical Summary

Problems solved by technology

[0005] The main technical problem to be solved by the present invention is to aim at the defect that most of the current food preservatives are chemical preservatives, long-term consumption of food preserved by such preservatives will cause harm to human health, and the residual chemical substances will also cause pollution to the environment , provides a kind of preparation method of natural bamboo leaf extract antimicrobial agent, and this method is to use fresh moso bamboo leaf as raw material, by removing yellow and rotten bamboo leaf, after drying and pulverizing, dissolving in phosphate buffer solution, magnetic stirring under ice-water bath is obtained Bamboo leaf pulp, then centrifuged at high speed to obtain the supernatant, salted out with ammonium sulfate to obtain crude antibacterial protein peptides, and finally obtained a kind of natural bamboo leaf extract antibacterial agent through dialysis, the antibacterial agent extracted by the present invention has biodegradability , no pollution to the environment