2results about How to "Biologically active" patented technology

This invention discloses a method for preparing and applying plant sulfonated small peptide PSK based on synthetic biology, belonging to the field of biotechnology. Based on the design concept of synthetic biology, this invention constructs an orthogonal synthetic system composed of genetic code expansion technology and SUMO fusion protease digestion technology. A specific PSK dotted vector and a pUltra-sY vector are co-transformed into *E. coli* to construct a genetically engineered strain. The sulfonated small peptide PSK precursor is expressed in this genetically engineered strain, and mature plant sulfonated small peptide PSK is obtained through specific hydrolysis by SUMO protease. This method significantly improves the synthesis efficiency and purity of sulfonated small peptides and has broad application prospects in agricultural production and biotechnology.

This invention provides recombinant humanized elastin, its preparation method, and its applications, relating to the field of bioengineering technology. Based on humanized elastin, this invention utilizes a combination design based on the functional domains of elastin to obtain recombinant humanized elastin, discovering novel bioactive functional fragments of humanized elastin. The recombinant humanized elastin of this invention integrates multiple elastin functional sites. Specifically, this recombinant humanized elastin exhibits significant effects in promoting cell proliferation and increasing the expression of collagen and elastin genes, demonstrating high safety and wide applicability in various scenarios, and providing a new active ingredient for anti-aging related products. Furthermore, the theoretical molecular weight of this recombinant humanized elastin is significantly smaller than that of natural full-length elastin, reducing obstacles for elastin to cross the skin barrier and exert its biological functions, thus exhibiting excellent biological efficacy.