1174results about How to "Biologically active" patented technology

The invention relates to a bio-organic compound fertilizer, which comprises the following components in part by weight: 10 to 30 parts of organic matter, 2 to 10 parts of compound microbial inoculants, 5 to 20 parts of humic acid, 5 to 20 parts of amino acid, 4 to 10 parts of chitin, 1 to 5 parts of polypeptide, 530 parts of nitrogen, phosphorus and potassium, 5 to 10 parts of secondary and trace elements, 5 to 15 parts of algae fertilizer, 5 to 10 parts of zeolite powder and 1 to 5 parts of synergistic agent. Through the scientific and rational formula, the bio-organic compound fertilizer achieves nutritive equilibrium and can satisfy the need of crop growth in nutrient. The microbial inoculants are added to quickly degrade natural macromolecular organic compounds into the organic fertilizer and decompose organic pollutants, which effectively improves the capacity of crops in the absorption of nutrient components, reduces the waste of the fertilizer and reduces the damage of the chemical fertilizer to soil to further achieve the nutritive equilibrium, satisfy the need of the crop growth in the nutrient, achieve the aims of low investment and high output value, improve the quality of the crops and improve the economic benefit brought by the crops.

A bio-based artificial quartz stone and a production process thereof are disclosed. The bio-based artificial quartz stone includes the following components by weight: 0-80% of quartz sand; 10-70% of quartz powder; 3-20% of curable resin; 0.05-3% of a curing agent; 0.01-40% of lignin; 0-5% of a crosslinking agent; 0.05-5% of a coupling agent; 0-1% of a promoter; 0-2% of a pigment; and 0-3% of a defoaming agent. According to the invention, the lignin is used in the artificial quartz, the environmental pollution of the lignin is reduced, the cost of the artificial quartz is reduced, and at the same time the weather resistance and light stability of the artificial quartz are improved, thus the artificial quartz has antibacterial ability, is green and environmental-friendly, and is suitable for using in building decoration of living environments.

The invention discloses a human fat mesenchymal stem cell extract and lyophilized powder and applications thereof. The human fat mesenchymal stem cell extract is prepared by the steps: collecting subcultured P3-P30 generations of human fat mesenchymal stem cells, culturing with cell growth liquid until 80% of cells are fused, removing culture liquid, washing the cells with a phosphate buffer solution for three times, adding into a serum-free culture solution, continuously culturing for 72 hours, collecting the serum-free culture solution, digesting uncollected cells with a digestion solution, crushing the cells with an ultrasonic crushing device, centrifuging, and then collecting supernatant; and mixing the collected serum-free culture solution and the collected supernatant, filtering, collecting filtrate, concentrating, and sterilizing. The human fat mesenchymal stem cell extract and the lyophilized powder thereof have multiple biological activities, can be used for preparing wound healing or cell repair medicaments and other biopharmaceutical products, and can also be used for preparing whitening products or skin anti-wrinkle products and other fine chemical products.

The invention relates to novel LSD1 inhibitors and application of the LSD1 inhibitors in drugs, in particular to application of the LSD1 inhibitors in preparing LSD1-resistant inhibitor drugs, drugs for treating tumor, virus infectious diseases, hematological diseases and the like. The chemical structural formula of the LSD1 inhibitors is shown in the description. For the compound I (one LSD1 inhibitor), the No. 2 carbons of benzene rings are connected with bromine atoms; the No. 4 carbons, No. 5 carbons, No. 3' carbons and No. 4' carbons are connected with hydroxyl groups; the English name of the compound I is (3',4'-dihydroxyphenyl)-(2-bromo-4,5-dihydroxyphenyl)methanone. For the compound II (the other LSD1 inhibitor), the No. 2 carbons and No. 2' carbons of benzene rings are connected with bromine atoms; the No. 4 carbons, the No. 5 carbons, the No. 4' carbons and No. 5' carbons are connected with hydroxyl groups; the English name of the compound II is (2'-bromo-4',5'-dihydroxyphenyl)-(2-bromo-4,5-dihydroxyphenyl)methanone.

The invention discloses a method for preparing a magnesium-based ceramic coating by micro-arc oxidation-electrophoretic deposition. The method comprises the following steps of: immersing preheated magnesium alloy taken as an anode in an electrolyte, taking a micro-arc oxidation stainless steel electrolytic cell as a cathode, adopting a direct-current pulsed micro-arc oxidation device, carrying out micro-arc oxidation for 10-25 minutes under the conditions that the electric current is 10-20A/dm<2>, the duty ratio is 37.5-50%, and the temperature of the electrolyte is 20-30 DEG C, taking out the anode, naturally drying, thus obtaining magnesium alloy subjected to micro-arc oxidation; immersing the magnesium alloy subjected to micro-arc oxidation and taken as the anode in electrophoretic deposition liquid, taking the stainless steel electrolytic cell as the cathode, adopting a pulsed power supply, carrying out electrophoretic deposition treatment for 10-30 minutes under the conditions that the voltage is 250-350V, and the duty ratio is 37.5-50%, naturally cooling, thus obtaining the magnesium-based ceramic coating. The adhesive force of the biological nanometer coating is obviously enhanced, the toughness and the corrosion resistance of the coating are improved, and the coating has certain biological activity.

The invention discloses a growth factor-loaded collagen group composite material and a preparation method thereof. The growth factor-loaded collagen group composite material is prepared from collagen, a growth factor, chitosan and sodium heparin, a dense layer collagen film and a loose layer collagen film form a double-layer structure, and a growth factor-loaded chitosan-heparin nano particle is compounded between the double layers. The dosage of the growth factor is 50-10,000ng/cm<2>; and the average thickness of the double-layer film is 1-4mm. The preparation method comprises the steps of preparing the dense collagen film and the loose collagen film, preparing the growth factor-loaded nano particle, compounding the collagen films and the nano particle, and the like. The collagen group composite material prepared by the invention has orient difference control slow release function on the loaded growth factor, has a slow release period longer than 20 days, maintains the activity of the growth factor and can be used for the restoration treatment of the disease defect tissue.

The invention relates to silk property-variable non-woven cotton with sericin and relative application. Said silk property-variable non-woven cotton via selection-modifying-blanching, and washing-carding-needling processes can be made into the silk property-variable non-woven cotton which will not lose silk. Said non-woven cotton can recover water-solubility and biological activity via protein recover treatment to supply the health function of silk protein, and apply the medical, hairdressing and daily consumption areas. Since said invention can keep a lot of sericin protein, therefore, it has lower cost, better fiber flexibility and thermal insulation than traditional silk non-woven cotton, and it can avoid de-gumming process to reduce environment pollution with better economic, social and zoology benefits

A process for preparing heparin oligose from heparinase includes such steps as culturing sphingobacterium (CGMCC No.0660), preparing non-cell coarse enzyme liquid, extracting and purifying heparinase, degradating heparin by the heparinase at 20-30 deg.C to obtain heparin oligose mixture, ultrafilter, gel filter for fractionation and evaporation concentration. The product has the activity of resisting smooth muscle hyperplasia.

The invention relates to a preparation method for a microarc oxidation/hydrothermal treatment composite film on the surface of a magnesium alloy. The problem that the existing magnesium alloy is poor in corrosion resistance and bioactivity is solved. A microarc oxidation film is prepared on the magnesium alloy by using a microarc oxidation method, and then a bioceramic film is obtained by using hydrothermal treatment. Hydrothermal reaction liquid is composed of sodium hydroxide, calcium acetate and sodium hexametapolyphosphate. In the preparation method, the microarc oxidation is combined with the hydrothermal treatment; calcium and phosphorus are introduced to the surface of the microarc oxidation film by using the hydrothermal treatment; in addition, holes in the ceramic film are blocked by using hydrothermal treatment; therefore, corrosive media are effectively prevented from entering; the corrosion resistance of the composite film is effectively improved; and the corrosion resistance of the composite film to simulated body fluid is greatly improved by approximately 2 orders of magnitude.

A hydrogel-conductive polymer nano-composite material with biological activity and the synthesis method relate to a biological activity material; firstly, polyethylene glycol and hydroxy acid are synthesized to obtain glycol-hydroxy-acid block copolymer under the catalysis of tin octoate; the copolymer, acryloyl chloride and triethylamine are reacted to get glycol-hydroxy-acid block copolymer terminated with acrylic group; the copolymer is then dissolved in water to get gel through photoinitiated crosslinking or free radical crosslinking; finally, the gel is swelled with pyrrole or aniline monomer solution to get the nano-composite material of the glycol-hydroxy-acid block copolymer and the polypyrole or polyaniline through the polymerization under the effect of initiators. Through the copolymerization with hydroxy acid, PEG is endowed with gel system biodegradability; besides, the honeycomb holes of the hydrogel film material can provide composite spaces for conductive polymers; the biological activity of the polypyrole and polyaniline enables the hydrogel to be endowed with biological activity after combination and has nano-enhancing effects to the gel system.

The invention relates to a novel nerve duct bracket and a preparation method and application thereof. Three-dimensional graphene or graphene derivative multichannel active nanofiber sponge is used asa stuffing inner core, and nanofibers in an axial direction are used as an outer pipe. The preparation method comprises the steps of performing ultrasonic dispersion on graphene or a graphene derivative into spinning liquid, performing electrostatic spinning, and performing drying so as to obtain a nanofiber film; performing shearing, and performing homogenization and crushing so as to obtain broken fiber mixed liquid; pouring the broken fiber mixed liquid into a multichannel mold, and performing freeze drying; performing cross-linked shaping; performing drying and shaping so as to obtain thethree-dimensional graphene or graphene derivative multichannel nanofiber sponge; and performing fixing in an axial direction spinning receiver, and performing axial direction spinning on an outer layer so as to obtain the novel nerve duct bracket. The novel nerve duct bracket is used for inducing repair and regeneration of nerve tissue. The novel nerve duct bracket disclosed by the invention is simple to operate and good in repeatability, and a new thinking is provided for repairing neurological deficits.

The invention provides a preparation method for linaclotide. The method includes: utilizing a standard Fmoc technology to connect a side chain protected amino acid with Wang resin, adding a condensing agent HBTU and alkali DIPEA, carrying out condensation reaction in a DMF solvent, employing a DMF solution containing 20% hexahydropiperidine to perform de-Fmoc protection, then conducting cutting from the solid phase resin, adding elemental iodine into a sodium phosphate buffer solution to carry out Cysteine oxidation, thus obtaining linaclotide. The preparation method for linaclotide provided by the invention is the technology for highyield synthesis of linaclotide, simplifies the cyclization process and enhances the cyclization yield, and the final product yield reaches 30%-60%. The preparation method has the advantages of simplicity, mild reaction conditions, high yield, and high product purity, is a feasible preparation method for industrialization of linaclotide, and provides good prospects for industrial production.

The invention relates to a preparation method of a recombinant spider silk protein/silver-bearing nano wound membrane. The preparation method comprises the steps of dissolving PVA (Polyvinyl Acetate) in H2O to prepare a PVA aqueous solution, conducting electrostatic spinning to prepare PVA nanofiber membrane, uniformly spraying a silver nitrate weak solution onto the PVA nanofiber membrane, drying, dissolving pNSR 16 in formic acid to prepare a formic acid solution of RGD (Arginine-Glycine-Aspartate)-spider silk protein, electrically spinning the formic acid solution of the RGD-spider silk protein into the PVA nanofiber membrane by an electrostatic spinning technology to form a pNSR 16 composite silver-bearing PVA nanofiber membrane, dyring, and obtaining the silver-bearing nano biological wound membrane. The membrane adopts natural protein materials and has bioactivity; as silver nitrate antibacterial components are introduced, the membrane has a significant antibacterial property and can kill most gram-negative bacteria and gram-positive bacteria; with the adoption of the electrostatic spinning technology, the preparation process is simple; and compared with medical gauze, the number of days required by complete healing of animal skin is reduced by approximately one third.

The invention relates to a method for preparing a titanium dioxide layer with a multi-level pore structure on the surface of medicinal titanium. The method provided by the invention comprises the following steps of: performing anode oxidation of a direct-current slow uniform-speed current raising mode on the medicinal titanium in acetic electrolyte, and performing secondary anode oxidation of a direct-current constant voltage mode in sulfuric acid or sodium acetate electrolyte to obtain the titanium dioxide layer with the multi-level pore structure, wherein the large-pore structure consists of staggered groove structures, the widths of grooves are 20 to 30 microns, the small-pore structure comprises submicron micropore structures densely distributed on the whole film, and the aperture is between dozens of and hundreds of microns. The method is simple and rapid, has simple and convenient operation, and is expected to become a surface modification method for medicinal titanium metal in the field of orthopedics, dentistry or plastic surgery.

The invention discloses organic titanium-potassium fertilizer and a preparation method thereof. The fertilizer is characterized in that the fertilizer takes water as solvent; and the compositions of the fertilizer in every liter of water are: 5 to 100g of organic chelated titanium, 75 to 200g of glucose, 600 to 800g of potassium citrate, 0 to 130g of borax or boric acid, 0 to 90g of humic acid or potassium humate, 0 to 60g of EDDHA chelating agent and 7 to 12g of auxiliary agent. The organic titanium-potassium fertilizer not only makes effective use of the prior fertilizer resources to change the status quo of constant high potassium fertilizer price in China, relieve contradictions between supply and demand and save resource, but also overcomes the differences between southern China and northern China in climate, soil, and the like, thereby playing a remarkable role in solving soil hardening, salinization and acidification. The fertilizer can also improve adverse circumstance resistance of crops, and is suitable for soil of various climates and various crops; moreover, the fertilizer better ensures yield increase of various crops and solves the problems of farmers such as low economic benefits, income increase, labor intensity reduction and the like; meanwhile, the fertilizer is propitious to advocate rational application of the fertilizer and improve the quality of farm products.

The invention discloses a preparation method and application of a porous hydrophilic denitrification biological carrier. The method comprises the following steps of: adding 75 mass parts of polyether polyol, 25 mass parts of polymer polyol, 3 mass parts of blackwash, 2 mass parts of dichloromethane, 0.25 mass part of surfactant, 0.25 mass part of amine catalyst, 0.3 mass part of tin catalyst and 5 mass parts of water into a 23 to 27 DEG C reaction kettle, and stirring; adding 52.5 mass parts of active agent, and uniformly stirring; adding 62.5 mass parts of toluene diisocynate, uniformly stirring, and performing reaction molding; and putting polyurethane foam subjected to reaction molding into a demolding tank, and blasting for demolding to obtain a magnetic biological denitrification carrier. The carrier prepared by the method has adsorption and catalytic oxidation effects and large specific surface area, a highly active biological film can be quickly formed, microbial cells can reproduce and grow on the huge surface of the carrier and absorb and degrade toxic or inhibiting substances, the carrier has a strong nitrification effect and a certain denitrification effect and can remove total nitrogen, and the residual sludge is obviously reduced.

The invention discloses an alimentary resistant starch, the process for preparing the starch, and the use of the starch in preparing oral administration colon targeting medicament control release vectors, wherein the starch preparing process comprises the steps of, (1) mixing the starch with water, (2) heavy pressure modifying the starch or starch milk, (3) reacting in a reactor, (4) subjecting the enzymolysis product yield to centrifugation, scouring, filtering, drying and disintegration.

A biobased artificial stone and a production process thereof are disclosed. The artificial stone includes the following components: inorganic filler, resin, a curing agent, an accelerating agent, a crosslinking agent, a coupling agent, an antifoaming agent, lignin and a pigment. The formula of the artificial stone includes by weight: 10-85% of the inorganic filler; 3-60% of the resin; 0.03-3% of the curing agent; 0-2% of the accelerating agent; 0.05-2% of the coupling agent; 0.05-5% of the crosslinking agent; 0-3% of the antifoaming agent; 0.01-40% of the lignin; and 0-2% of the pigment. According to the invention, the lignin is used in the artificial stone, the lignin is turned into wealth from waste, the environmental pollution of the lignin is reduced, the cost of the artificial stone is reduced, and at the same time the weather resistance and light stability of the artificial stone are improved, thus the artificial stone has antibacterial ability, is green and environmental-friendly, and is suitable for building decoration of living environments.

The invention discloses an application of ionic liquid which serves as a steel corrosion inhibitor. The imidazolyl lysine salt ionic liquid serves as a corrosion inhibitor, only the corrosion inhibitor is required to be added into a solution contacted with steel, and the corrosion inhibitor is easy to operate and good in corrosion inhibition effect. The corrosion inhibitor is environment-friendly, high in solubility and biodegradability and good in corrosion inhibition effect, and solves the problems that the conventional corrosion inhibitor has large toxic and side effects, low biodegradation, poor environment friendliness and the like.

A beta-elemene five-element azacyclic derivative is disclosed, which is prepared through reaction of beta-elmene on hypochlorite or NBS to obtain intermediate, and reacting on 1,3,4-oxadiazole or 1,2,4-triazole thiol in DMF or DMSO solvent to obtain beta-elemene, 1,3,4-oxadiazole (or 1,2,4-triazole) derivative.

The invention provides alginate fiber as well as a preparation method and application of the alginate fiber. According to the invention, the ion-exchange property of the traditional calcium alginate fiber is improved, and an alginate and propylene glycol alginate blended fiber material with stronger hygroscopicity is provided. The invention provides the alginate fiber which is prepared by blending 50-99.5% of alginate and 0.5-50% of propylene glycol alginate by weight. The invention further provides a preparation method and application of the alginate fiber. Water-soluble non-ion type propylene glycol alginate is added to the blended fiber, the hygroscopicity of the fiber and medical dressing processed by the fiber are improved; and the fiber is particularly suitable for wound with more bleeding purulence, can prolong the service life of the product, reduces the replacing times of the dressing, reduces the patient pain and promotes the wound healing.

The invention, which belongs to the field of biochemistry, relates to a tumor-targeting functionalized quantum dot and a preparation method thereof. The functionalized quantum dot consists of folic acid, cyclodextrin and quantum dot, and part of folic acid molecules are contained in the hydrophobic cavity of the cyclodextrin to form folic acid-cyclodextrin inclusion compound, which is then combined with the surface of the quantum dot. In the process of preparing the functionalized quantum dot, the folic acid is first combined with the Gamma-cyclodextrin to form the inclusion compound, and the Gamma-cyclodextrin-folic acid inclusion compound is then modified on the surface of the quantum dot, so that the dual-function nano material with excellent fluorescent characteristic and tumor-targeting property is produced. Since the Gamma-cyclodextrin is used to include the folic acid, the water-solubility and dispersibility of the folic acid are improved. The folic acid and the quantum dot are connected together by an effective method, and the produced fluorescent probe can be used for biological research on tumor cells. The preparation method is simple, the cost is low, and the screening of a variety of tumor cells and an in-vivo experiment on mice indicate that the material is characterized by low toxicity, high selectivity and the like.

The preparation process of sintered porous titanium artificial bone with bioceramic coating belongs to the field of biomedicine engineering technology. The preparation process includes stereo weaving of titanium metal fiber to form bionic structure model simulating human body's bone trabecula, pre-pressing formation and vacuum sintering to prepare porous titanium artificial bone, sol-gel process to form gradient and composite coating on the surface of the porous titanium artificial bone. The present invention can protect titanium metal skeleton, avoid titanium ion dissociation and make the artificial bone possess biological characteristic, and may be used in the clinical repair of large bone defect.