12results about How to "Biologically active" patented technology

The invention discloses a soluble expression and purification method of recombinant human bone morphogenetic protein in a prokaryotic system. A gene sequence of a recombinant human bone morphogenetic protein (rhBMP) is spliced at the 3'terminal of a His-Linker-GB1 gene sequence, inserted into a prokaryotic expression system expression vector and transferred into a prokaryotic competent cell to be highly expressed in the cell in a soluble form, and finally, the expressed His-Linker-GB1-BMP fusion protein does not need to be subjected to inclusion body renaturation, so that the recombinant human bone morphogenetic protein (rhBMP) is obtained. The high-purity fusion protein can be obtained through one-step metal chelating chromatography (adsorption-elution mode), and the GB1 tag has small molecular weight and hardly affects the spatial structure and biological activity of rhBMP, so that the situation that an inclusion body is formed by expression of rhBMP in a traditional escherichia coli system and expression is performed in a structure-free and activity-free inclusion body form is avoided, and the expression efficiency is greatly improved. The method is simple and convenient to operate, is suitable for large-scale amplification, and has the characteristics of short period, high purity, stable process and the like.

This invention discloses a method for preparing and applying plant sulfonated small peptide PSK based on synthetic biology, belonging to the field of biotechnology. Based on the design concept of synthetic biology, this invention constructs an orthogonal synthetic system composed of genetic code expansion technology and SUMO fusion protease digestion technology. A specific PSK dotted vector and a pUltra-sY vector are co-transformed into *E. coli* to construct a genetically engineered strain. The sulfonated small peptide PSK precursor is expressed in this genetically engineered strain, and mature plant sulfonated small peptide PSK is obtained through specific hydrolysis by SUMO protease. This method significantly improves the synthesis efficiency and purity of sulfonated small peptides and has broad application prospects in agricultural production and biotechnology.

The invention discloses a preparation method of alkyl-substituted polyfluorinated aromatic hydrocarbon based on Hanus ester, and belongs to the field of organic synthesis. The invention solves the problems that the existing polyfluoroaryl substituted alkane synthesis substrate has large range limitation, needs metal catalysis, and needs harsh reaction conditions such as high temperature, no water, no oxygen and the like. The method comprises the following steps: uniformly mixing polyfluorinated aromatic hydrocarbon, alkyl-substituted Hanus ester, an Ir (dFCF3ppy) dtbpyPF6 photocatalyst, anhydrous potassium carbonate and an organic solvent, and then carrying out blue illumination reaction. The method is used for preparing the alkyl-substituted polyfluorinated aromatic hydrocarbon based on the Hanus ester.

The invention relates to the technical field of application of agricultural pollination insects, in particular to a field pollination method for megachile megachile. The invention provides a field pollination method for megachile megachile. The method comprises the following steps: a preparation stage: preparing incubation, arranging a honeycomb and preparing a nest plate; in the pollination stage, megachile clover is released, nesting conditions are observed, and disease and pest control is carried out; and a recovery treatment stage. The method can effectively improve the fruiting performance of alfalfa.

The invention discloses a composite ice inhibitor, a vitrification protective agent and a blood vessel cryopreservation method, and belongs to the field of cryopreservation or vitrification preservation of vascularized tissues and organs. According to the volume of 100 mL of the composite ice inhibiting agent, the composite ice inhibiting agent is prepared from 10 to 500 g of polyvinyl alcohol, 10 to 500 g of polyglycerol, 10 to 500 g of trehalose, 10 to 500 g of L-proline, 10 to 500 g of polyvinylpyrrolidone, 10 to 500 g of polyethylene glycol 6000 and the balance of ultrapure water, and the total volume of the composite ice inhibiting agent is 100 mL. According to the volume of 1L of the vitrification protective agent, the vitrification protective agent contains 1.65 to 2.2 mol of dihydric alcohol, 2.3 to 3.1 mol of dimethyl sulfoxide, 2.3 to 3.1 mol of formamide, 12 to 57 mmol of a pH buffer solution, 0.39 to 1.93 mmol of a composite ice inhibitor and the balance of ultrapure water, wherein the total volume is 1L; the compound ice inhibiting agent has the advantages that the solution damage is reduced, the ice inhibiting performance is better, and the damage to cells caused by extra osmotic pressure is reduced under the minimum use metering. The composite ice inhibitor is especially suitable for cryopreservation of endothelial cells, smooth muscle cells and blood vessels.

The invention belongs to the technical field of culture substrates, and particularly relates to a granular material prepared on the basis of biomass leavening and mineral substances and application of the granular material as a culture substrate. The granular material provided by the invention comprises the following raw materials: mineral powder, a biomass fermentation product, a polysaccharide-acid compound and a binder, the polysaccharide-acid compound is composed of chitosan and potassium humate, the polysaccharide-acid compound is loaded on mineral powder by adopting a vacuum negative pressure impregnation method, and finally the granular material is prepared. The granular material is stable in structure and high in moisture and fertilizer retention capacity, has biological activity and is environmentally friendly.

This invention belongs to the field of biomedical technology and discloses the use of fluoropyridoxal in the preparation of drugs for anticancer purposes, including: use in drugs for the prevention and / or alleviation and / or treatment of cancer. The cancer is ovarian cancer. The drug for the prevention and / or alleviation and / or treatment of cancer comprises fluoropyridoxal and a pharmaceutically acceptable carrier. The dosage form of the drug for the prevention and / or alleviation and / or treatment of cancer is tablets, granules, capsules, suspensions, syrups, emulsions, or injections. Fluoropyridoxal induces senescence-like death, cell cycle arrest, and apoptosis in cancer cells. Fluoropyridoxal acts by inducing apoptosis in cancer cells. The fluoropyridoxal of this invention can effectively inhibit the proliferation and growth of human ovarian cancer cell lines SKOV3, OVCAR3, 3AO, and ES-2. The fluoropyridoxal of this invention can be used as an effective drug for the treatment of ovarian cancer.

The invention provides a bifidobacterium-nano-selenium radiotherapy sensitizer as well as a preparation method and application thereof. The bifidobacterium-nano-selenium radiotherapy sensitizer is prepared from a Na2SeO3 solution, a Vc solution and bifidobacterium longum. The radiotherapy sensitizer can accurately target tumors, remodel an immunosuppression microenvironment and enhance the radiotherapy effect, and is safe and effective. The synthesis mode of the material is simple and effective, and the thought of using a biological material as a radiotherapy sensitizer is widened; the nano-material has biological activity and is selectively accumulated in a tumor, so that the defects of poor targeting property and poor metabolism effect of a general nano-material serving as a radiotherapy sensitizer are overcome; bifidobacterium longum in human intestinal tracts is selected as a substrate of the system, so that the defects that conventional attenuated or engineering bacteria are large in side effect and poor in biocompatibility when serving as a delivery substrate are overcome.

The invention belongs to but not limited to the technical field of genetic engineering, and discloses an Anguilla japonica Activin A recombinant protein, and an expression method and application thereof. The anguilla japonica Activin A recombinant protein comprises an anguilla japonica Activin A mature peptide amino acid sequence, a 6 * histidine tag at the N end and SUMO dissolution promoting polypeptide; the amino acid sequence of the anguilla japonica Activin A recombinant protein is SEQ ID NO: 1, and the nucleotide sequence of the coding gene of the anguilla japonica Activin A recombinant protein is SEQ ID NO: 3. The prokaryotic expression vector pET-His-SUMO-Activin A of the Japanese eel Activin A is constructed for the first time, the Activin A recombinant protein of which the amino acid sequence is consistent with the mature peptide of the Japanese eel Activin A can be obtained, and the Activin A recombinant protein can be applied to ovarian development regulation and artificial propagation research of the Japanese eel.

The invention belongs to the technical field of biomedical materials, and particularly relates to an antibacterial dressing compounded with an extracellular trap and a preparation method of the antibacterial dressing. The antibacterial dressing of the composite extracellular trap comprises hydrogel and the extracellular trap loaded in the hydrogel, the antibacterial dressing can actively capture and efficiently kill bacteria including drug-resistant bacteria, a novel dressing biological sterilization mechanism is achieved, biocompatibility is good, and wound healing can be accelerated.

This invention provides recombinant humanized elastin, its preparation method, and its applications, relating to the field of bioengineering technology. Based on humanized elastin, this invention utilizes a combination design based on the functional domains of elastin to obtain recombinant humanized elastin, discovering novel bioactive functional fragments of humanized elastin. The recombinant humanized elastin of this invention integrates multiple elastin functional sites. Specifically, this recombinant humanized elastin exhibits significant effects in promoting cell proliferation and increasing the expression of collagen and elastin genes, demonstrating high safety and wide applicability in various scenarios, and providing a new active ingredient for anti-aging related products. Furthermore, the theoretical molecular weight of this recombinant humanized elastin is significantly smaller than that of natural full-length elastin, reducing obstacles for elastin to cross the skin barrier and exert its biological functions, thus exhibiting excellent biological efficacy.

The invention provides a method for synthesizing a hydrophis cyanocinctus antibacterial peptide Hc-CATH through multi-copy integration and high-density fermentation, and belongs to the technical field of genetic engineering and bioengineering. According to the invention, a series-connection two-copy plasmid is constructed, pichia pastoris is taken as a host, two-copy series-connection protein is successfully expressed, and the expression yield of the hydrophis cyanocinctus antibacterial peptide Hc-CATH is improved by 2-3 times. According to the present invention, the hydrophis cyanocinctus antibacterial peptide Hc-CATH can be synthesized at a high level through the construction and optimization of the two-copy integration method and the optimization of the fermentation process, and the method has advantages of strong versatility, high expression efficiency, biological activity, low cost, easy large-scale production, and the like, and can be used for the preparation of the hydrophis cyanocinctus antibacterial peptide Hc-CATH.