44 results about "Peptide expression" patented technology

To provide an inhibitor for suppressing expression of an antibacterial peptide in brown planthopper and an application thereof.SOLUTION: A dsNlPCE4 inhibitor designed based on the NlPCE4 gene of the brown planthopper is obtained. The inhibitor, when applied to brown planthoppers inoculated on their body surface with Metarhizium anisopliae, significantly increases the mortality of the brown planthopper. The present invention combines two technical strategies for pest control, biocontrol fungi and RNA interference (RNAi), to provide a new target for brown planthopper control technology based on cooperative mediation of RNAi.SELECTED DRAWING: Figure 5

The invention relates to an artificial nucleic acid molecule comprising at least one 5′ UTR element which is derived from a TOP gene, at least one open reading frame, and preferably at least one histone stem-loop. Optionally the artificial nucleic acid molecule may further comprise, e.g. a poly(A)sequence, a poyladenylation signal, and / or a 3′ UTR. The invention further relates to the use of such an artificial nucleic acid molecule in gene therapy and / or genetic vaccination.

The invention relates to a novel application of a compound, in particular to an application of piceatannol in preparation of an animal host defense peptide expression promoter. The invention provides an application of piceatannol in preparation of a host defense peptide expression promoter, wherein piceatannol shown in a formula I or a derivative thereof is used as a pharmacodynamic active component to prepare the host defense peptide expression promoter in a required dosage form. The piceatannol improves the animal immune function by inducing host endogenous defense peptide expression, is not prone to causing bacterial drug resistance, can be developed into a novel feed additive, replaces antibiotics and meets the requirements of modern animal husbandry.

The invention discloses an antibacterial peptide modified bionic nanoparticle targeting delivery system as well as a construction method and application thereof. The antibacterial peptide modified bionic nanoparticle targeting delivery system comprises a recombinant antibacterial peptide LL-37, a macrophage membrane and an enzyme cascade reaction system, the recombinant antibacterial peptide LL-37 is expressed on the macrophage cell membrane to form a macrophage cell membrane modified by the recombinant antibacterial peptide LL-37; the enzyme cascade reaction system is wrapped by the macrophage membrane modified by the recombinant antibacterial peptide LL-37, so that the bionic nanoparticle targeted delivery system modified by the antibacterial peptide is formed; wherein a Toll-like receptor 2 and a Toll-like receptor 4 are highly expressed on the surface of a macrophage cell membrane; the enzyme cascade reaction system comprises a nano enzyme with catalase-like activity and a hydrogen peroxide-producing biological enzyme. The antibacterial peptide modified bionic nanoparticle targeting delivery system disclosed by the invention can be used for treating infectious bone defects such as periodontitis, peri-implantitis, infectious fracture, osteomyelitis and the like.

Disclosed are a synthesis system for a multi-copy peptide without redundant sequences, and a use. The synthesis system comprises a synthetic expression vector, wherein the synthetic expression vector comprises a tag-(linker-target polypeptide)n, n=1-10, n represents the number of copies, and the tag has an amino acid sequence selected from KPYDGP, KSKGEE, MTMITDSLAVVLQ, GFILGFIL or HHHHHH. The fusion tag enhances the expression of multi-copy polypeptides and results in a higher theoretical yield of the target polypeptide.