14 results about "Trilobatin" patented technology

The invention provides an anti-anemia traditional Chinese medicine composition as well as a preparation method and application thereof. The anti-anemia traditional Chinese medicine composition is prepared from lithocarpus litseifolius, fructus gardeniae and rhizoma gastrodiae. The main active component dihydrochalcone (such as phlorizin, trilobatin, phloretin and the like) of lithocarpus litseifolius can improve iron metabolism by regulating a hepcidin-iron transporter axis; gardenoside in gardenia can target the liver to optimize iron distribution, and gastrodia elata polysaccharide can regulate immunity and improve the marrow microenvironment; the three forms a full-chain regulation and control network of intestinal tract iron absorption, liver iron utilization and bone marrow hematopoiesis microenvironment together, and the anti-anemia effect is enhanced in multiple dimensions.

The application belongs to the technical field of electrochemical analysis and detection, and discloses a preparation method and application of an electrochemiluminescence sensor for specifically detecting trilobatin. The specific operation includes: (1) preparing a composite material of nitrogen-doped carbon quantum dots (N-CDs) and a silver-based metal organic framework (AgMOF) (AgMOF@N-CDs); (2) modifying the AgMOF@N-CDs on a glassy carbon electrode to obtain AgMOF@N-CDs / GCE; and (3) based on a three-electrode system, using the AgMOF@N-CDs / GCE as a working electrode, a platinum wire as an auxiliary electrode, and Ag / AgCl as a reference electrode to construct an electrochemiluminescence sensor for detecting trilobatin. The preparation of the electrochemiluminescence sensor is simple, the analysis speed is relatively fast, the detection range is relatively wide, the sensitivity is relatively high, and the selectivity is relatively good.

The application belongs to the technical field of electrochemical analysis and detection, and discloses a molecular imprinting electrochemiluminescence sensor, a preparation method thereof and application of the sensor in selective detection of trilobatin. The operation comprises the following steps: (1) a prepared g-C3N4@NiMOF composite material is modified on the surface of a glassy carbon electrode; (2) the g-C3N4@NiMOF / GCE is placed in a phosphate buffer solution containing o-phenylenediamine and TRI, and o-PD and TRI are electropolymerized on the electrode surface through cyclic voltammetry in a three-electrode system to obtain MIP-p(o-PD)-TRI / g-C3N4@NiMOF / GCE; and (3) TRI in the polymerization film is eluted to form a specific imprinting cavity, and a MIP-p(o-PD) / g-C3N4@NiMOF / GCE sensor is obtained. The molecular imprinting electrochemiluminescence sensor has the advantages of simple operation, fast analysis speed, good selectivity and high sensitivity in the detection of TRI.

The application discloses a capillary electrophoresis quantitative analysis method of active ingredients of Wuobi Shanyao pills. The method comprises the following steps: pretreating and purifying the powder of the test sample Wuobi Shanyao pills by MSPD-SPE, and quantitatively determining the active ingredients of the test sample, i.e., loganin, geniposidic acid, trilobatin and verbascoside by CE-UV. The MSPD with high extraction efficiency, the SPE with strong purification capacity and the CE with less consumption of organic solvents are combined for the first time, and are used for green, efficient and accurate content determination of the four components (loganin, geniposidic acid, trilobatin and verbascoside) in Wuobi Shanyao pills. In the sample preparation process and in the CE separation and determination, the consumption of organic solvents is far less than the amount consumed in the prior art.

The application belongs to the technical field of electrochemical analysis and detection, and discloses a preparation method and application of an electrochemiluminescence sensor for selectively detecting trilobatin. The specific operation includes: (1) preparing a composite material (ZnPTC@MoS2) of a rhombus sheet-shaped zinc-based metal organic framework (ZnPTC) and molybdenum disulfide (MoS2); (2) modifying the ZnPTC@MoS2 on a glassy carbon electrode to obtain a ZnPTC@MoS2 / GCE; and (3) based on a three-electrode system, constructing an electrochemiluminescence sensor for specifically detecting trilobatin by taking the ZnPTC@MoS2 / GCE as a working electrode, a platinum wire as an auxiliary electrode, and Ag / AgCl as a reference electrode. The preparation of the electrochemiluminescence sensor is simple, the analysis speed is relatively fast, the detection range is relatively wide, the selectivity is relatively good, and the sensitivity is relatively high.

The invention discloses high-activity hypoglycemic strigose hydrangea juvenile leaf extract microcapsule powder and a preparation method thereof.The microcapsule powder is prepared through the method comprising the following steps that firstly, a high-voltage pulsed electric field (1-3 kV / cm) is adopted to assist alkalescent electrolyzed water to extract strigose hydrangea juvenile leaf, and a strigose hydrangea juvenile leaf extract (STE) is obtained; and mixing the extract with a wall material composed of at least two of maltodextrin, Arabic gum, inulin and arabinoxylan according to a mass ratio of 1: 0.25-1: 1.25, homogenizing, and spray-drying at an air inlet temperature of 140 + / -5 DEG C and an air outlet temperature of 80 + / -5 DEG C. The obtained microcapsule powder contains various high-content active ingredients such as 0.3 wt%-1.0 wt% of trilobatin and 0.3 wt%-1.0 wt% of phlorizin, and the embedding rate is high (for example, the embedding rate of total flavonoids is 67.53%-88.84%). According to the product disclosed by the invention, the bitter taste of the strigose hydrangea juvenile leaf is remarkably improved, the active ingredients can be effectively protected, the high inhibition activity on alpha-glucosidase and alpha-amylase can be maintained through simulated gastrointestinal tract digestion, the blood sugar reducing function is outstanding, and the product is suitable for preparing food, health care products or medicines with the blood sugar reducing function.

The invention belongs to the technical field of biology, and discloses a method for producing phloretin through fermentation of eurotium cristatum. On the basis of pretreatment of lithocarpus litseifolius, eurotium cristatum is used as a fermentation inoculant, and after aerobic solid fermentation is performed on the eurotium cristatum, trilobatin and phlorizin in the lithocarpus litseifolius are converted into phloretin. According to the method, eurotium cristatum is inoculated into lithocarpus litseifolius by utilizing a microbial solid-state fermentation technology, high-content phloretin is obtained after fermentation is finished, and the method is simple, convenient, short in production period and suitable for subsequent large-scale production.

The invention belongs to the field of medicinal chemistry, and discloses an application of a ferrous ion complex of dihydrochalcone compounds (phlorizin, PHL) and trilobatin, TLB) in prevention and treatment of food-borne microorganisms, and a preparation method of the ferrous ion complex of the dihydrochalcone compounds (phlorizin, PHL) and the trilobatin, TLB). The phlorizin-ferrous ion complex and the trilobatin-ferrous ion complex have relatively good DPPH (1, 1-diphenyl-2-picrylhydrazyl) scavenging capacity and hydroxyl radical scavenging capacity; the phlorizin-ferrous ion complex and the trilobatin-ferrous ion complex can slow down oxidation of edible oil and fat; the phlorizin-ferrous ion complex and the trilobatin-ferrous ion complex have different inhibitory activities on the tested food-borne microorganisms; when the compound is combined with the cefdinir for use, the phlorizin-ferrous ion and trilobatin-ferrous ion complexes can synergistically promote the antibacterial effect of the cefdinir, and the use dosage of the cefdinir is reduced. The two have the potential of being further researched and developed into a novel edible antibacterial agent.

The invention discloses a protein related to phloretin enzyme activity as well as a coding gene and application thereof. The technical problem to be solved is how to provide a phloretin enzyme activity related protein. The invention specifically discloses any one of the following applications: A1) application of the protein in catalyzing phloretin to generate phlorizin and / or trilobatin and / or preparing a product for catalyzing phloretin to generate phlorizin and / or trilobatin; a2) application in production of phlorizin and / or preparation of a product for producing phlorizin; and A3) producing trilobatin and / or preparing a product for producing the trilobatin. The protein is any one of the following: B1) a protein with an amino acid sequence as shown in a sequence 2; b2) a protein which is obtained by substitution and / or deletion and / or addition of amino acid residues of the protein B1), has 80% or more of identity with the protein B1) and has the same function as the protein B1); and B3) a fusion protein obtained by connecting the N terminal or / and the C terminal of B1) or B2) with a protein tag. The method can be used for industrial production.

The invention discloses a process method for extracting trilobatin from sweet tea tender leaves, and belongs to the technical field of trilobatin separation. The method comprises the following steps: countercurrent extraction, chromatographic separation and crystallization purification, and specifically comprises the following steps: by taking tender leaves of strigose hydrangea juvenile leaves as a raw material, treating the tender leaves of strigose hydrangea juvenile leaves by adopting an aqueous solution of mixed enzyme and a surfactant, and countercurrent extraction, so that the solubility of trilobatin in water is improved, the trilobatin is fully dissolved out, and the use of a large amount of organic extraction solvents is avoided; and based on the molecular sieve action of macroporous resin and the principle that the solubility of trilobatin in a low-temperature aqueous solution is reduced, the removal of components such as polysaccharide, protein and phloretin in the sweet tea tender leaves is realized, and high-purity trilobatin crystals are obtained. The method has the advantages of simple and efficient process, no need of specific equipment and suitability for industrial production, and has a wide application prospect in the field of trilobatin extraction.

The invention provides a phloretin-trilobatin co-amorphous substance and a solid dispersion thereof, and a preparation method and pharmaceutical application thereof. The two active ingredients of phloretin and trilobatin can be directly prepared into an amorphous substance under the condition of not using auxiliary materials, so that the solubility of phloretin and trilobatin is improved. On the basis, micromolecular sugar alcohol is further introduced, the solid dispersion is prepared under the condition that other auxiliary materials are not added, and the solubility of the two is more remarkably improved. The preparation process is simple, efficient, low in cost and easy to industrialize. The obtained product not only can enhance the water solubility of phloretin and trilobatin at the same time, has a better sweetener application prospect, but also shows good in-vitro blood fat reducing activity, and the phloretin and the trilobatin show a remarkable synergistic effect, so that a new strategy is provided for developing an efficient compound blood fat reducing preparation.

The application discloses a method for promoting the growth of leaf of Lithocarpus polystachyus, synthesis of dihydrochalcone and reduction of heavy metal content. The method uses selenium-rich yeast as a leaf surface treatment agent of the Lithocarpus polystachyus, and can simultaneously promote the growth of leaf, synthesis of dihydrochalcone and reduction of the content of heavy metals arsenic and lead in the leaf. The content of trilobatin in the young leaf and mature leaf can be increased by 190% and 83% respectively, the proportion of the number of new young leaf plants can be increased to 52%, and the content of lead and arsenic can be reduced by 92.2% and 347.7% respectively. The method has remarkable quality improvement and yield increasing effects, is simple and easy to implement, has short treatment time, is environment-friendly and pollution-free, has high economic benefits, is suitable for functional component improvement and heavy metal pollution prevention and control of the Lithocarpus polystachyus, and has wide application prospects.

The invention belongs to the field of biological manufacturing, and discloses a self-assembled multienzyme complex for trilobatin synthesis as well as a construction method and application of the self-assembled multienzyme complex. According to the invention, phloretin 4 '-O-glycosyl transferase MtP4' GT and sucrose synthase GmSUS are respectively combined and connected with scaffold protein through flexible peptides according to different strategies, and a plurality of recombinant plasmids capable of simultaneously coding MtP4 'GT genes and GmSUS genes are constructed; and respectively introducing the plurality of recombinant plasmids into an escherichia coli host to obtain a plurality of recombinant bacterium multienzyme complexes. The yield of trilobatin generated through catalytic conversion of phloretin is used as a screening index, and an optimized single plasmid co-expression strain pET25b-SpyTag-Linker-PGT-SpyCatch-Linker-SUS-1 / BL21start (DE3) is obtained through screening and used for synthesis of the trilobatin. Meanwhile, the invention constructs a green solvent reaction system for green production of trilobatin. The self-assembled multienzyme complex and green solvent reaction system constructed by the invention has high efficiency and economical efficiency in the aspect of synthesizing trilobatin by directional catalytic conversion of phloretin, and has potential application value for promoting the development of a green biological manufacturing process.