83 results about "Hesperetin" patented technology

The invention is applicable to the technical field of biological medicines, provides application of a natural compound of a targeted colon cancer drug resistance related protein, and particularly relates to application in preparation of drugs for preventing or treating colon cancer. According to the invention, a natural small molecule compound neohesperidin dihydrochalcone targeting the colon cancer drug resistance related protein TAGLN is screened out, and a new scheme is provided for reversing colon cancer drug resistance, so that the situation that no drug is available to the target spot is solved. Secondly, the invention also provides a scheme for combined use of neohesperidin dihydrochalcone and oxaliplatin, the scheme can significantly enhance the killing effect of oxaliplatin on drug-resistant colon cancer, and a new choice is provided for clinical treatment of colon cancer.

The invention belongs to the technical field of medicines, and particularly relates to a flavonoid compound-meglumine co-amorphous substance and a preparation method thereof. The flavonoid compounds comprise baicalein, hesperetin, naringenin, kaempferol, quercetin, isoliquiritigenin and the like, and the co-amorphous substance consists of a single flavonoid compound and meglumine and is prepared by adopting a melting shock cooling method or a rotary evaporation method. The miscibility of the flavonoid compounds and the meglumine is screened through Hansen solubility. The crystal diffraction characteristics, thermodynamic properties and intermolecular interaction of the co-amorphous substance are measured through PXRD, DSC and FTIR, and it is shown that the six flavonoid compounds and meglumine successfully form a co-amorphous system. The solubility and the dissolution rate of the six flavonoid compound-meglumine co-amorphous substance are obviously superior to those of flavonoid bulk drugs, and the physical stability of the formed co-amorphous substance is obviously superior to that of a single amorphous drug, so that the co-amorphous substance shows good patent medicine performance.

The invention discloses a collagen peptide and a dressing preparation formed by combining the collagen peptide and hesperetin. And the collagen peptide is obtained by carrying out enzymolysis on amino acid sequences as shown in SEQ ID NO.5-SEQ ID NO.8. The collagen peptide is prepared from the collagen peptide. The polypeptide and the preparation thereof have the functions of resisting oxidation and repairing skin injury.

Compositions that contain flavonoids such as hesperidin, quercetin, hesperetin, and rutin and methods of treating viral diseases such as coronavirus (e.g., SARS COV-2) infection, influenza virus infection, rhinovirus infection, and human metapneumovirus infection.

The application belongs to the technical field of biological medicine, and provides application of a natural compound targeting colon cancer drug resistance related protein, in particular, application in preparation of a drug for preventing or treating colon cancer; the application screens out a natural small-molecule compound hesperetin dihydrochalcone targeting colon cancer drug resistance related protein TAGLN, and provides a new scheme for reversing colon cancer drug resistance, so as to solve the situation that no drug is available for the target point. In addition, the application further provides a scheme in which hesperetin dihydrochalcone is combined with oxaliplatin, and the scheme can significantly enhance the killing effect of oxaliplatin on drug-resistant colon cancer, thereby providing a new choice for clinical treatment of colon cancer.

The invention relates to a spleen-tonifying, appetite-stimulating and digestion-promoting traditional Chinese medicine preparation UPLC detection method and a fingerprint spectrum, and belongs to the technical field of medicine detection. The invention aims to solve the technical problems that the existing quality control method of the spleen-tonifying, appetite-promoting and digestion-promoting pills lacks comprehensive and objective analysis on the spleen-tonifying, appetite-promoting and digestion-promoting pills, and the problems of low precision, poor reproducibility, low efficiency and the like during detection of related components in the prior art are solved. According to the technical scheme, the UPLC detection method is characterized in that a mixed standard substance of gallic acid, 5-hydroxymethylfurfural, chlorogenic acid, sinapine thiocyanate, sinapic acid, hyperoside, 3, 6-dimustard acyl sucrose, hesperidin, naringenin, nobiletin and hesperetin is taken as a contrast, an acetonitrile-0. 1% formic acid aqueous solution is taken as a mobile phase, gradient elution is carried out, and the content of chlorogenic acid in the sample is detected. The qualitative and quantitative detection of the effective components of the spleen-tonifying, appetite-promoting and digestion-promoting pill is realized.

The present application relates to a kind of methyltransferase CicOMT10 mutant, preparation method, biological material and application.The methyltransferase mutant is mutated with the amino acid sequence such as SEQ ID NO:1 CicOMT10 enzyme, and the mutation includes: the asparagine of 179th is mutated to alanine;And / or, the threonine of 316th is mutated to alanine.The methyltransferase mutant of the present application can efficiently convert eriodictyol into hesperetin.

The invention provides a glycosidase mutant and application thereof, the amino acid sequence of the mutant is compared with the amino acid sequence SEQ ID NO.1 of a wild enzyme, in the amino acid sequence SEQ ID NO.1, five sites of M102K, P171L, V203G, T269Y and K299D are respectively subjected to single mutation, pairwise combined mutation, three combined mutation, four combined mutation or one of five combined mutation; the novel glycosidase mutant industrial enzyme is used for synthesis and preparation of hesperetin. The glycosidase mutant enzyme constructed by the invention has the characteristics of low enzyme cost, short conversion time, simple process operation and the like, and has a wide prospect of large-scale industrial application.

The present disclosure generally relates to flavonoid compounds, such as hesperetin, and their use for enhancing the perception of alcohol (ethanol) in food, beverages, and other comestible compositions. In certain aspects, the disclosure provides the use of such flavonoid compounds to enhance an alcoholic (ethanolic) flavor of a beverage, for example, a low-alcohol or zero-alcohol beverage. In certain other aspects, the disclosure provides low-alcohol or zero-alcohol beverages containing such flavonoid compounds.

The present invention provides a sweetener composition with improved taste quality of a sweet substance. The present invention relates to a sweetener composition containing the following components (A), (B), and (C): (A) at least one γ-glutamyl peptide selected from the group consisting of a compound represented by the formula (I): γ-Glu-X-Gly (I) wherein X is an amino acid residue or an amino acid derivative residue, and a compound represented by the formula (II): γ-Glu-Y (II) wherein Y is an amino acid residue or an amino acid derivative residue, or a salt thereof, (B) at least one compound selected from the group consisting of naringenin, hesperetin, and pinocembrin, or a salt thereof, and (C) a sweet substance.

The present invention primarily relates to a mixture comprising or consisting of a) D-allulose and b) a taste modifying compound selected from the group consisting of hesperetin, hersperetindihydrochalcone, phloretin, glycosylated rubusosides, balansines, phyllodulcin, eriodictyol, homoeriodictyol, matairesinol, and mixtures thereof. A further aspect of the present invention relates to a food or beverage product comprising a mixture according to the present invention.

The present disclosure provides compositions comprising a glycoside hydrolase and one or both of hesperidin and hesperetin, wherein an active site of the glycoside hydrolase comprises first, second, third, fourth, and fifth regions as described herein. The present disclosure also relates to methods for producing a polyphenol aglycone, comprising contacting a phenolic glycoside with a glycoside hydrolase described herein. Also provided are host cells and heterologous expression systems capable of expressing a heterologous glycoside hydrolase, and nucleic acids encoding the glycoside hydrolase.

The invention relates to the technical field of new application of a natural orange peel extract, in particular to a bacteriostatic agent of the natural orange peel extract and application of the bacteriostatic agent. Bacteriostatic experiments find that the natural extract hesperetin and / or hesperidin of the orange peel has a good bacteriostatic effect on most of aquaculture indicator bacteria. Animal experiments after the hesperetin and / or hesperidin are prepared into the disease-resistant feed find that the hesperetin or hesperidin can effectively improve the resistance of fishes to streptococcus iniae infection. A 5 * MIC hesperetin group shows the best protection effect, the protection rate reaches 60%, and the protection rate of streptococcus iniae fed with a hesperidin group is 46%. Therefore, the hesperetin and / or the hesperidin which is the natural extract of the orange peel can be used as a bacteriostatic agent or a bacteriostatic feed in aquaculture, new application of the hesperetin and the hesperidin in aquaculture is developed, and the hesperetin and the hesperidin have important significance in controlling and preventing streptococcicosis.

The invention discloses bifidobacterium adolescentis for producing various active small molecules by fermenting white truffle, promoting collagen synthesis of skin cells and resisting aging, and belongs to the technical field of microorganisms. According to the present invention, the Bifidobacterium adolescentis CCFM1492 obtained through screening can degrade eriodictyol and cyanidin-3-O-glucoside chloride in the white truffle, and can produce a variety of active small molecules such as hesperetin, phloretin, ganoderenic acid D, gallic acid, myricetin and the like through fermentation of the white truffle, such that the active small molecules can be used for degrading the white truffle and the cyanidin-3-O-glucoside chloride in the white truffle; according to the present invention, with the application of the Bifidobacterium adolescentis CCFM1492 fermented white truffle fermentation broth, the promotion effect of the white truffle on the skin cell collagen synthesis and the anti-aging can be improved so as to improve the skin quality, such that the Bifidobacterium adolescentis CCFM1492 fermented white truffle fermentation broth has the huge application prospect in the preparation of the product for preventing or treating the skin quality.

A flavor composition is provided. The composition includes a dihydrochalcone selected from the group consisting of hesperetin dihydrochalcone, hesperetin dihydrochalcone 4"-beta-D-glucoside, and combinations thereof, and an aromatic ingredient selected from the group consisting of 4-hydroxy-2H-furan-5-one, ethyl furanone, maltol, furfural, 5-methylfurfural, vanillin, benzaldehyde, and combinations thereof.

The invention discloses a plant lactobacillus for producing various active small molecules by fermenting truffles and resisting skin cell aging in multiple mechanisms, and belongs to the technical field of microorganisms. The lactobacillus plantarum CCFM1494 obtained by screening can be used for fermenting the white truffle, degrading cyanidin-3-O-glucoside chloride, and generating a plurality of active small molecules such as hesperetin, phloretin, ganoderenic acid D, gallic acid, myricetin, protocatechuic acid and the like; meanwhile, after the white truffle is fermented by the plant lactobacillus CCFM1494, the promotion effect of the white truffle on the synthesis of skin collagen and the improvement effect on cell senescence can be improved. Therefore, the lactobacillus plantarum CCFM1494 has a huge application prospect in preparation of products for improving skin quality.

The present application relates to the technical field of antibacterial drugs, and specifically discloses a pleuromutilin derivative and a use thereof, wherein the pleuromutilin derivative has a structure of being connected to a side chain of C14 of pleuromutilin by a mercaptoethylamine, piperazine or oxygen atom from a dihydroflavone or oxime ether derivative thereof. The present application introduces dihydroflavone (naringenin and hesperetin) and oxime ether derivatives thereof having antibacterial effects into the side chain of C14 of pleuromutilin by taking pleuromutilin as a raw material. The dihydroflavone and oxime ether derivatives thereof have great potential as an advantageous structural unit due to their unique C6-C3-C6 basic skeleton (C2 and C3 are single bonds) and extensive antibacterial activity. The dihydroflavone and oxime ether derivatives thereof are combined with pleuromutilin to synthesize a plurality of dihydroflavone pleuromutilin derivatives having excellent antibacterial activity. The present application provides a new choice for designing a new generation of pleuromutilin derivatives by a hybrid strategy, improving drug properties while improving activity, and providing an anti-infective drug.

The invention discloses saccharomyces cerevisiae and a method for efficiently synthesizing neohesperidin by using cane sugar. According to the saccharomyces cerevisiae, on the basis of ZQH01, FjTAL derived from Flavobacterium johnsoniae, AtC4H, AtPAL2 and AtATR2 derived from Arabidopsis thaliana and CYB5 derived from yeast are inserted into a genome of the ZQH01, and the saccharomyces cerevisiae is prepared by the following steps of: adding the FjTAL, the AtC4H, the AtPAL2 and the AtATR2 into the genome of the ZQH01; according to the invention, a sucrose phospholysis double-carbon-source system is introduced into the saccharomyces cerevisiae, sucrose can be directly converted into fructose and glucose-1-phosphoric acid, the fructose is specially used for cell growth and hesperetin synthesis, the glucose-1-phosphoric acid directionally supplies UDP-glucose, and a carbon flow is physically separated to eliminate flux competition caused by a Crab effect. The saccharomyces cerevisiae disclosed by the invention can be used for efficiently synthesizing neohesperidin by using cane sugar, and has important significance on green and efficient acquisition of citrus flavonoids.

The present application discloses the use of hesperetin in the preparation of a drug or feed additive for treating or preventing porcine enveloped virus infection. The drug is hesperetin or an oral liquid, granule, tablet, or powder prepared by adding a solvent or excipient with hesperetin as the active ingredient, and the dosage is 2-10 mg / kg of pig body weight; or hesperetin is directly added to the feed, and the addition amount is 0.01%-0.03% of the total weight of the pig feed. The present application uses hesperetin or a composition including hesperetin as a drug or feed additive, which has high safety, no toxic and side effects, and has a significant effect of inhibiting the replication of enveloped viruses such as porcine epidemic diarrhea virus, porcine reproductive and respiratory syndrome virus, and African swine fever virus, and reducing the virulence of porcine enveloped virus. It overcomes the disadvantages such as immune failure that may occur after vaccination and virus mutation caused by the use of antiviral drugs, and effectively reduces the incidence and mortality of piglets infected with porcine enveloped virus.

The invention discloses hesperetin additive feed capable of improving intestinal health of micropterus salmoides and a preparation method of the hesperetin additive feed. The feed comprises the following raw materials: 40%-50% of fish meal, 8%-12% of chicken meal, 5%-10% of cottonseed meal, 5%-8% of rapeseed meal, 6%-12% of soybean meal, 6%-10% of wheat flour, 1%-7% of fish oil, 1%-7% of soybean oil, 0.3%-1% of monocalcium phosphate, 0.1%-1% of composite vitamins, 0.1%-1% of mixed minerals, 0.1%-0.5% of choline chloride, 0.1%-1% of vitamin C, 0.1%-2% of gelatin, 1%-12% of microcrystalline cellulose and 0.1%-0.5% of hesperetin. The hesperetin is added into the feed raw materials, so that the intestinal oxidation resistance of the largemouth bass can be remarkably improved, the damage of intestinal inflammation reaction to the intestinal tract can be improved, and the intestinal health of the largemouth bass can be protected.

The invention discloses a hesperetin derivative containing a thiosemicarbazone structure and application of the hesperetin derivative, the molecular formula of the compound is C18H19N3O5S, the biological activity determination result shows that the compound has good anti-oxidation and anti-alpha-glucosidase activity, the IC50 value of DPPH scavenging activity is 6.17 mu g / mL, and the activity of the hesperetin derivative is equivalent to that of a control agent vitamin C. The hesperetin derivative containing the thiosemicarbazone structure and the application of the hesperetin derivative containing the thiosemicarbazone structure have good anti-oxidation and anti-alpha-glucosidase activity. The IC50 value of ABTS clearance activity is 0.04 mu g / mL, and the activity is improved by 200 times compared with that of a control medicament vitamin E; the IC50 value of the alpha-glucosidase inhibitory activity is 4.53 [mu] g / mL, which is improved by 51 times compared with the activity of a control agent acarbose; the structural formula of the hesperetin derivative containing the thiosemicarbazone structure is shown in the specification.

The present invention discloses flavonoid 4'-O-methyltransferase and its application in the production of hesperetin, belonging to the field of genetic engineering and bioengineering technology. The present invention screened and identified new flavonoid 4'-O-methyltransferases derived from tea-branch orange and Huazhou pomelo; the flavonoid 4'-O-methyltransferases CrcOMT-2 and CgtOMT-3 obtained by screening were integrated and expressed in the multi-copy site rDNA and ZETA site on the genome of Yarrowia lipolytica YE26, respectively, to achieve the de novo synthesis of hesperetin. The Yarrowia lipolytica Z02 constructed by the present invention achieved the de novo synthesis of 130.2 mg / L hesperetin in a 5L fermentor, which is also the highest yield of hesperetin de novo synthesis in microorganisms reported, laying the foundation for the subsequent biosynthesis of flavonoids.

The present invention belongs to the technical field of enzyme engineering, and particularly relates to a sucrose synthase mutant and its application. The sucrose synthase mutant described in the present invention is obtained by the following mutations on the amino acid sequence of sucrose synthase shown in SEQ ID NO.1: valine at position 159 is mutated to threonine. The sucrose synthase mutant has high enzyme activity and can be applied to the biocatalytic synthesis of neohesperidin. The present invention also provides a method for preparing neohesperidin using the sucrose synthase mutant. Using hesperetin-7-O-glucoside, sucrose, and uridine diphosphate as substrates, and adding the whole-cell crude enzyme solution of rhamnosyltransferase, rhamnose synthase, and sucrose synthase, neohesperidin is catalytically obtained with a relatively high conversion rate.

The present disclosure provides compositions comprising a glycoside hydrolase and one or both of hesperidin and hesperetin, wherein an active site of the glycoside hydrolase comprises first, second, third, fourth, and fifth regions as described herein. The present disclosure also relates to methods for producing a polyphenol aglycone, comprising contacting a phenolic glycoside with a glycoside hydrolase described herein. Also provided are host cells and heterologous expression systems capable of expressing a heterologous glycoside hydrolase, and nucleic acids encoding the glycoside hydrolase.

The application belongs to the technical field of natural products, and discloses M-dCNs nano-enzyme as well as a preparation method and application thereof. The M-dCNs nano-enzyme comprises the following components in percentage by mass: hesperetin (HST) 84-87%, KPLH1130 5-8%, Fe 3+ 5-8%. The application constructs a metal-natural product complex, utilizes HST to complex with Fe 3+ , does not need to add a carrier or be modified through a covalent bond, can self-assemble to form nano-enzyme with uniform morphology, has small particle size, a drug loading capacity of 100%, and high stability, eliminates the dependence on a carrier and avoids the limitation of a drug loading rate, effectively improves the activity of HST by self-assembling to form nano-enzyme, exhibits liver aggregation targeting, greatly improves the treatment effect on liver damage, and can be applied to the preparation of a medicine for preventing and / or treating liver damage.

The invention discloses application of hesperetin in preparation of a medicine for resisting leiocassis longirostris herpesvirus. The hesperetin can significantly reduce expression of CCV replication related genes and synthesis of proteins, inhibit CCV-induced cytopathic effects and have obvious prevention and treatment effects on CCV infection, the anti-CCV activity of the hesperetin shows obvious drug concentration dependence, and the action mechanism of the hesperetin can finally inhibit CCV infection by inhibiting an internalization process of CCV. As a natural active component, hesperetin has the characteristics of low toxicity, low residue, low cost, greenness and safety, and can be used for preventing and treating leiocassis longirostris herpes viruses harmful to leiocassis longirostris culture by utilizing the biological activity of hesperetin, so that a new path is provided for the development and research of green biological agents for aquaculture, and the new application of hesperetin is expanded.

This invention discloses the application of citrus flavonoids (NOB) or SR1078 in the preparation of drugs to improve salivary gland function, particularly in improving the circadian rhythm of salivary glands damaged by ionizing radiation. Oral administration of a specific dose of NOB or SR1078 can significantly promote salivary gland function. Furthermore, it does not interact with other drugs with anti-inflammatory or regenerative functions. It can be administered as a single dose or multiple times consecutively; it can be administered after injury, and this flexible administration method does not affect the tumor radiotherapy process.

The application discloses an environment-friendly plant-based degradable high molecular material and a preparation method thereof, and relates to the technical field of high molecular materials. In the preparation of the environment-friendly plant-based degradable high molecular material, chitosan is sequentially reacted with 5-aldehyde furan-3-boronic pinacol ester and 1-bromo-1,2,2-triphenyl ethylene to obtain modified chitosan; hesperetin is reacted with 4-(chloromethyl)-1,3-dioxolane-2-ketone to obtain functionalized hesperetin; dimethyl adipate, a phosphorus-containing diacid ester monomer and 1,6-hexanediamine are subjected to polycondensation to obtain polyamide; the polyamide is reacted with formaldehyde to obtain modified polyamide; the modified polyamide, the modified chitosan, the functionalized hesperetin and 1,5,7-triazabicyclo[4.4.0]dec-5-ene are mixed and injection molded to obtain the environment-friendly plant-based degradable high molecular material. The environment-friendly plant-based degradable high molecular material prepared by the application has excellent anti-aging, flame-retardant and mechanical properties.

The invention relates to a methyltransferase CicOMT10 mutant, a preparation method, a biological material and application. The methyltransferase mutant is formed by mutation of CicOMT10 enzyme with an amino acid sequence as shown in SEQ ID NO: 1. The mutation comprises the following steps: asparagine at the 179th site is mutated into alanine; and / or, threonine at the 316th site is mutated into alanine. The methyltransferase mutant disclosed by the invention can be used for efficiently converting eriodictyol into hesperetin.

A flavor composition is provided. The composition includes a dihydrochalcone selected from the group consisting of hesperetin dihydrochalcone, hesperetin dihydrochalcone 4″-beta-D-glucoside, and combinations thereof, and an aroma ingredient selected from the group consisting of furonol, ethyl furanone, maltol, furfural, 5-methyl furfural, vanillin, benzaldehyde, and combinations thereof.