53 results about "Hesperetin" patented technology

The invention is applicable to the technical field of biological medicines, provides application of a natural compound of a targeted colon cancer drug resistance related protein, and particularly relates to application in preparation of drugs for preventing or treating colon cancer. According to the invention, a natural small molecule compound neohesperidin dihydrochalcone targeting the colon cancer drug resistance related protein TAGLN is screened out, and a new scheme is provided for reversing colon cancer drug resistance, so that the situation that no drug is available to the target spot is solved. Secondly, the invention also provides a scheme for combined use of neohesperidin dihydrochalcone and oxaliplatin, the scheme can significantly enhance the killing effect of oxaliplatin on drug-resistant colon cancer, and a new choice is provided for clinical treatment of colon cancer.

Compositions that contain flavonoids such as hesperidin, quercetin, hesperetin, and rutin and methods of treating viral diseases such as coronavirus (e.g., SARS COV-2) infection, influenza virus infection, rhinovirus infection, and human metapneumovirus infection.

The application belongs to the technical field of biological medicine, and provides application of a natural compound targeting colon cancer drug resistance related protein, in particular, application in preparation of a drug for preventing or treating colon cancer; the application screens out a natural small-molecule compound hesperetin dihydrochalcone targeting colon cancer drug resistance related protein TAGLN, and provides a new scheme for reversing colon cancer drug resistance, so as to solve the situation that no drug is available for the target point. In addition, the application further provides a scheme in which hesperetin dihydrochalcone is combined with oxaliplatin, and the scheme can significantly enhance the killing effect of oxaliplatin on drug-resistant colon cancer, thereby providing a new choice for clinical treatment of colon cancer.

The invention relates to a spleen-tonifying, appetite-stimulating and digestion-promoting traditional Chinese medicine preparation UPLC detection method and a fingerprint spectrum, and belongs to the technical field of medicine detection. The invention aims to solve the technical problems that the existing quality control method of the spleen-tonifying, appetite-promoting and digestion-promoting pills lacks comprehensive and objective analysis on the spleen-tonifying, appetite-promoting and digestion-promoting pills, and the problems of low precision, poor reproducibility, low efficiency and the like during detection of related components in the prior art are solved. According to the technical scheme, the UPLC detection method is characterized in that a mixed standard substance of gallic acid, 5-hydroxymethylfurfural, chlorogenic acid, sinapine thiocyanate, sinapic acid, hyperoside, 3, 6-dimustard acyl sucrose, hesperidin, naringenin, nobiletin and hesperetin is taken as a contrast, an acetonitrile-0. 1% formic acid aqueous solution is taken as a mobile phase, gradient elution is carried out, and the content of chlorogenic acid in the sample is detected. The qualitative and quantitative detection of the effective components of the spleen-tonifying, appetite-promoting and digestion-promoting pill is realized.

The present application relates to a kind of methyltransferase CicOMT10 mutant, preparation method, biological material and application.The methyltransferase mutant is mutated with the amino acid sequence such as SEQ ID NO:1 CicOMT10 enzyme, and the mutation includes: the asparagine of 179th is mutated to alanine;And / or, the threonine of 316th is mutated to alanine.The methyltransferase mutant of the present application can efficiently convert eriodictyol into hesperetin.

The invention provides a glycosidase mutant and application thereof, the amino acid sequence of the mutant is compared with the amino acid sequence SEQ ID NO.1 of a wild enzyme, in the amino acid sequence SEQ ID NO.1, five sites of M102K, P171L, V203G, T269Y and K299D are respectively subjected to single mutation, pairwise combined mutation, three combined mutation, four combined mutation or one of five combined mutation; the novel glycosidase mutant industrial enzyme is used for synthesis and preparation of hesperetin. The glycosidase mutant enzyme constructed by the invention has the characteristics of low enzyme cost, short conversion time, simple process operation and the like, and has a wide prospect of large-scale industrial application.

The present disclosure generally relates to flavonoid compounds, such as hesperetin, and their use for enhancing the perception of alcohol (ethanol) in food, beverages, and other comestible compositions. In certain aspects, the disclosure provides the use of such flavonoid compounds to enhance an alcoholic (ethanolic) flavor of a beverage, for example, a low-alcohol or zero-alcohol beverage. In certain other aspects, the disclosure provides low-alcohol or zero-alcohol beverages containing such flavonoid compounds.

The present invention provides a sweetener composition with improved taste quality of a sweet substance. The present invention relates to a sweetener composition containing the following components (A), (B), and (C): (A) at least one γ-glutamyl peptide selected from the group consisting of a compound represented by the formula (I): γ-Glu-X-Gly (I) wherein X is an amino acid residue or an amino acid derivative residue, and a compound represented by the formula (II): γ-Glu-Y (II) wherein Y is an amino acid residue or an amino acid derivative residue, or a salt thereof, (B) at least one compound selected from the group consisting of naringenin, hesperetin, and pinocembrin, or a salt thereof, and (C) a sweet substance.

The present disclosure provides compositions comprising a glycoside hydrolase and one or both of hesperidin and hesperetin, wherein an active site of the glycoside hydrolase comprises first, second, third, fourth, and fifth regions as described herein. The present disclosure also relates to methods for producing a polyphenol aglycone, comprising contacting a phenolic glycoside with a glycoside hydrolase described herein. Also provided are host cells and heterologous expression systems capable of expressing a heterologous glycoside hydrolase, and nucleic acids encoding the glycoside hydrolase.

The invention discloses bifidobacterium adolescentis for producing various active small molecules by fermenting white truffle, promoting collagen synthesis of skin cells and resisting aging, and belongs to the technical field of microorganisms. According to the present invention, the Bifidobacterium adolescentis CCFM1492 obtained through screening can degrade eriodictyol and cyanidin-3-O-glucoside chloride in the white truffle, and can produce a variety of active small molecules such as hesperetin, phloretin, ganoderenic acid D, gallic acid, myricetin and the like through fermentation of the white truffle, such that the active small molecules can be used for degrading the white truffle and the cyanidin-3-O-glucoside chloride in the white truffle; according to the present invention, with the application of the Bifidobacterium adolescentis CCFM1492 fermented white truffle fermentation broth, the promotion effect of the white truffle on the skin cell collagen synthesis and the anti-aging can be improved so as to improve the skin quality, such that the Bifidobacterium adolescentis CCFM1492 fermented white truffle fermentation broth has the huge application prospect in the preparation of the product for preventing or treating the skin quality.

A flavor composition is provided. The composition includes a dihydrochalcone selected from the group consisting of hesperetin dihydrochalcone, hesperetin dihydrochalcone 4"-beta-D-glucoside, and combinations thereof, and an aromatic ingredient selected from the group consisting of 4-hydroxy-2H-furan-5-one, ethyl furanone, maltol, furfural, 5-methylfurfural, vanillin, benzaldehyde, and combinations thereof.

The invention discloses a plant lactobacillus for producing various active small molecules by fermenting truffles and resisting skin cell aging in multiple mechanisms, and belongs to the technical field of microorganisms. The lactobacillus plantarum CCFM1494 obtained by screening can be used for fermenting the white truffle, degrading cyanidin-3-O-glucoside chloride, and generating a plurality of active small molecules such as hesperetin, phloretin, ganoderenic acid D, gallic acid, myricetin, protocatechuic acid and the like; meanwhile, after the white truffle is fermented by the plant lactobacillus CCFM1494, the promotion effect of the white truffle on the synthesis of skin collagen and the improvement effect on cell senescence can be improved. Therefore, the lactobacillus plantarum CCFM1494 has a huge application prospect in preparation of products for improving skin quality.

The present application relates to the technical field of antibacterial drugs, and specifically discloses a pleuromutilin derivative and a use thereof, wherein the pleuromutilin derivative has a structure of being connected to a side chain of C14 of pleuromutilin by a mercaptoethylamine, piperazine or oxygen atom from a dihydroflavone or oxime ether derivative thereof. The present application introduces dihydroflavone (naringenin and hesperetin) and oxime ether derivatives thereof having antibacterial effects into the side chain of C14 of pleuromutilin by taking pleuromutilin as a raw material. The dihydroflavone and oxime ether derivatives thereof have great potential as an advantageous structural unit due to their unique C6-C3-C6 basic skeleton (C2 and C3 are single bonds) and extensive antibacterial activity. The dihydroflavone and oxime ether derivatives thereof are combined with pleuromutilin to synthesize a plurality of dihydroflavone pleuromutilin derivatives having excellent antibacterial activity. The present application provides a new choice for designing a new generation of pleuromutilin derivatives by a hybrid strategy, improving drug properties while improving activity, and providing an anti-infective drug.

The invention discloses saccharomyces cerevisiae and a method for efficiently synthesizing neohesperidin by using cane sugar. According to the saccharomyces cerevisiae, on the basis of ZQH01, FjTAL derived from Flavobacterium johnsoniae, AtC4H, AtPAL2 and AtATR2 derived from Arabidopsis thaliana and CYB5 derived from yeast are inserted into a genome of the ZQH01, and the saccharomyces cerevisiae is prepared by the following steps of: adding the FjTAL, the AtC4H, the AtPAL2 and the AtATR2 into the genome of the ZQH01; according to the invention, a sucrose phospholysis double-carbon-source system is introduced into the saccharomyces cerevisiae, sucrose can be directly converted into fructose and glucose-1-phosphoric acid, the fructose is specially used for cell growth and hesperetin synthesis, the glucose-1-phosphoric acid directionally supplies UDP-glucose, and a carbon flow is physically separated to eliminate flux competition caused by a Crab effect. The saccharomyces cerevisiae disclosed by the invention can be used for efficiently synthesizing neohesperidin by using cane sugar, and has important significance on green and efficient acquisition of citrus flavonoids.

The invention discloses a hesperetin derivative containing a thiosemicarbazone structure and application of the hesperetin derivative, the molecular formula of the compound is C18H19N3O5S, the biological activity determination result shows that the compound has good anti-oxidation and anti-alpha-glucosidase activity, the IC50 value of DPPH scavenging activity is 6.17 mu g / mL, and the activity of the hesperetin derivative is equivalent to that of a control agent vitamin C. The hesperetin derivative containing the thiosemicarbazone structure and the application of the hesperetin derivative containing the thiosemicarbazone structure have good anti-oxidation and anti-alpha-glucosidase activity. The IC50 value of ABTS clearance activity is 0.04 mu g / mL, and the activity is improved by 200 times compared with that of a control medicament vitamin E; the IC50 value of the alpha-glucosidase inhibitory activity is 4.53 [mu] g / mL, which is improved by 51 times compared with the activity of a control agent acarbose; the structural formula of the hesperetin derivative containing the thiosemicarbazone structure is shown in the specification.

The present invention discloses flavonoid 4'-O-methyltransferase and its application in the production of hesperetin, belonging to the field of genetic engineering and bioengineering technology. The present invention screened and identified new flavonoid 4'-O-methyltransferases derived from tea-branch orange and Huazhou pomelo; the flavonoid 4'-O-methyltransferases CrcOMT-2 and CgtOMT-3 obtained by screening were integrated and expressed in the multi-copy site rDNA and ZETA site on the genome of Yarrowia lipolytica YE26, respectively, to achieve the de novo synthesis of hesperetin. The Yarrowia lipolytica Z02 constructed by the present invention achieved the de novo synthesis of 130.2 mg / L hesperetin in a 5L fermentor, which is also the highest yield of hesperetin de novo synthesis in microorganisms reported, laying the foundation for the subsequent biosynthesis of flavonoids.

The present disclosure provides compositions comprising a glycoside hydrolase and one or both of hesperidin and hesperetin, wherein an active site of the glycoside hydrolase comprises first, second, third, fourth, and fifth regions as described herein. The present disclosure also relates to methods for producing a polyphenol aglycone, comprising contacting a phenolic glycoside with a glycoside hydrolase described herein. Also provided are host cells and heterologous expression systems capable of expressing a heterologous glycoside hydrolase, and nucleic acids encoding the glycoside hydrolase.

The application belongs to the technical field of natural products, and discloses M-dCNs nano-enzyme as well as a preparation method and application thereof. The M-dCNs nano-enzyme comprises the following components in percentage by mass: hesperetin (HST) 84-87%, KPLH1130 5-8%, Fe 3+ 5-8%. The application constructs a metal-natural product complex, utilizes HST to complex with Fe 3+ , does not need to add a carrier or be modified through a covalent bond, can self-assemble to form nano-enzyme with uniform morphology, has small particle size, a drug loading capacity of 100%, and high stability, eliminates the dependence on a carrier and avoids the limitation of a drug loading rate, effectively improves the activity of HST by self-assembling to form nano-enzyme, exhibits liver aggregation targeting, greatly improves the treatment effect on liver damage, and can be applied to the preparation of a medicine for preventing and / or treating liver damage.

This invention discloses the application of citrus flavonoids (NOB) or SR1078 in the preparation of drugs to improve salivary gland function, particularly in improving the circadian rhythm of salivary glands damaged by ionizing radiation. Oral administration of a specific dose of NOB or SR1078 can significantly promote salivary gland function. Furthermore, it does not interact with other drugs with anti-inflammatory or regenerative functions. It can be administered as a single dose or multiple times consecutively; it can be administered after injury, and this flexible administration method does not affect the tumor radiotherapy process.

The application discloses an environment-friendly plant-based degradable high molecular material and a preparation method thereof, and relates to the technical field of high molecular materials. In the preparation of the environment-friendly plant-based degradable high molecular material, chitosan is sequentially reacted with 5-aldehyde furan-3-boronic pinacol ester and 1-bromo-1,2,2-triphenyl ethylene to obtain modified chitosan; hesperetin is reacted with 4-(chloromethyl)-1,3-dioxolane-2-ketone to obtain functionalized hesperetin; dimethyl adipate, a phosphorus-containing diacid ester monomer and 1,6-hexanediamine are subjected to polycondensation to obtain polyamide; the polyamide is reacted with formaldehyde to obtain modified polyamide; the modified polyamide, the modified chitosan, the functionalized hesperetin and 1,5,7-triazabicyclo[4.4.0]dec-5-ene are mixed and injection molded to obtain the environment-friendly plant-based degradable high molecular material. The environment-friendly plant-based degradable high molecular material prepared by the application has excellent anti-aging, flame-retardant and mechanical properties.

A flavor composition is provided. The composition includes a dihydrochalcone selected from the group consisting of hesperetin dihydrochalcone, hesperetin dihydrochalcone 4″-beta-D-glucoside, and combinations thereof, and an aroma ingredient selected from the group consisting of furonol, ethyl furanone, maltol, furfural, 5-methyl furfural, vanillin, benzaldehyde, and combinations thereof.

The invention relates to a continuous countercurrent ultrasonic vortex extraction system and a method for extracting hesperetin from citrus peel, and the continuous countercurrent ultrasonic vortex extraction system comprises a raw material pretreatment unit used for crushing a dried citrus peel raw material; an inlet of the slurry blending tank is connected with the raw material pretreatment unit, the slurry blending tank is used for receiving the crushed raw materials and mixing the crushed raw materials with a solvent to prepare slurry, and an outlet of the slurry blending tank is connected with a first conveying pump. The ultrasonic vortex tube type reactor is of a vertical or inclined tubular structure, and the ultrasonic transducers are uniformly distributed in the axial direction of the ultrasonic vortex tube type reactor. According to the technical scheme, the solvent consumption is reduced through high solvent utilization rate brought by the countercurrent process. Therefore, the production cost is reduced, and the environmental protection pressure of subsequent waste liquid treatment is greatly reduced; the method has significant progress in the aspects of extraction efficiency and solvent saving, can be suitable for large-scale continuous automatic production, and has advancement and industrial application value.

The invention discloses a composition helpful for controlling in-vivo fat and application thereof, and relates to the technical field of biology. The hesperidin composition is prepared from hesperidin and lactobacillus johnsonii (Lactobacillus johnsonii), and the hesperidin composition is prepared from the hesperidin and the lactobacillus johnsonii. Research finds that the composition can achieve synergistic up-regulation of gene and protein expression of adipose tissue PGC1alpha and UCP1 and increase the body surface temperature; the composition can also significantly improve the bioavailability of hesperidin and improve the concentration of hesperetin and other metabolites in serum; the composition can also optimize the intestinal microecology, improve the intestinal flora structure, increase the lactobacillus colonization rate, enrich the abundance of beneficial bacteria and promote the generation of short-chain fatty acids; the composition can also significantly improve lipid metabolism disorder of an organism, reduce fat accumulation, regulate adiponectin and leptin levels, control fat in the body and realize effective weight loss management.

A flavor composition is provided. The composition includes a dihydrochalcone selected from the group consisting of hesperetin dihydrochalcone, hesperetin dihydrochalcone 4″-beta-D-glucoside, and combinations thereof, and an aroma ingredient selected from the group consisting of furonol, ethyl furanone, maltol, furfural, 5-methyl furfural, vanillin, benzaldehyde, and combinations thereof.

The invention provides a bifidobacterium longum subsp. Longum HX-BL11 capable of improving exercise fatigue, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.32960, and the preservation date is December 6, 2024. Meanwhile, the invention further provides a fermentation composition obtained by fermenting spina date seeds, hawthorn fruits, perilla seeds and dried tangerine or orange peel with the bifidobacterium longum subsp. Longum HX-BL11, and a fermentation product of the fermentation composition is rich in ferulic acid, phlorizin and hesperidin. The probiotic preparation and the fermentation product can improve the swimming exercise time of mice, improve indexes of fatigue damage degrees such as BUN, LD, LDH and CK-MB in the exercise mice, improve related indexes of energy metabolism such as GLU, MG and ATP, improve the exercise tolerance, improve antioxidant indexes such as T-SOD level and MDA content, improve the antioxidant capacity, play a synergistic effect and improve the health care effect of the mice. Therefore, the effect of relieving exercise fatigue is achieved. And meanwhile, the probiotic preparation of the bifidobacterium longum subsp. Longum HX-BL11 and the fermented product are simple in preparation method, can be produced in a large scale, and have a good application prospect.

The invention provides a functional feed additive for relieving development retardation of piglets as well as a preparation method and application of the functional feed additive. The functional feed additive for relieving development retardation of piglets comprises the following components in parts by weight: 30-50 parts of a polysaccharide-mesoporous silica nanoparticle compound, 0.1-2 parts of tartary buckwheat oligopeptide and 0.1-1 part of hesperetin. The functional feed additive provided by the invention can regulate the redox balance of piglet bodies, enhance the immunity of the piglet bodies, improve the intestinal mucosa injury of the piglets and promote the intestinal development of the piglets, so that the development retardation of the piglets is relieved, and the healthy growth of the piglets with the development retardation is promoted.

The present invention relates, inter alia, to a method for reducing the consumption of sweet foods or reducing the desire or craving for sweet foods in a human or animal subject, comprising: providing to the subject for consumption an edible item comprising caloric carbohydrate and hesperetin, wherein the subject has a low sweet taste threshold.

The invention relates to the technical field of antibacterial drugs, and particularly discloses a pleuromutilin derivative and application thereof, and the pleuromutilin derivative is structurally characterized in that flavanone or an oximido / oxime ether derivative of flavanone is connected to a side chain of pleuromutilin C14 through mercaptoethylamine, piperazine or an oxygen atom. According to the invention, pleuromutilin is taken as a raw material, flavanone (naringenin and hesperetin) with an antibacterial effect and an oximido / oxime ether derivative thereof are introduced into a pleuromutilin C14 side chain, and the flavanone and the oximido / oxime ether derivative thereof show huge potential as a dominant structural unit due to a specific C6-C3-C6 basic skeleton (C2 and C3 sites are single bonds) and wide antibacterial activity; the flavonoid compound is combined with pleuromutilin to synthesize a plurality of dihydroflavonoid pleuromutilin derivatives with excellent antibacterial activity, and a new choice is provided for designing a new generation of pleuromutilin derivatives through a hybridization strategy, improving the drug characteristics of the pleuromutilin derivatives while improving the activity and anti-infection drugs of the pleuromutilin derivatives.

The application discloses a rhamnose-based hydrolase mutant, wherein a lysine at position 513 of rhamnose lyase TpeRha is mutated; the site is mutated into an amino acid with a hydrophobic side chain; the hydrolysis efficiency of the mutant K513A and the mutant K513V is respectively 2.39 times and 6.27 times higher than that of the wild type; the application further discloses a preparation method of the rhamnose-based hydrolase mutant; and the application further discloses application of the rhamnose-based hydrolase mutant, wherein the rhamnose-based hydrolase mutant is used for preparing a composition of hesperetin-7-O-glucoside, hesperetin or a combination of the two.

The invention discloses a hesperetin multilevel lipid nanoparticle delivery system based on glyceryl dioleate and a preparation method of the hesperetin multilevel lipid nanoparticle delivery system. The blank lipid nanoparticles with stable physicochemical properties are successfully prepared by systematically optimizing key parameters such as the composition proportion of the glyceryl dioleate and the Tween 80, the ultrasonic emulsification time and the like. On the basis, the lipid-drug ratio and the coating concentration of the chitosan and the bovine serum albumin are further accurately regulated and controlled, and three delivery systems of the hesperetin lipid nanoparticles, the chitosan modified hesperetin lipid nanoparticles and the bovine serum albumin-chitosan double-layer coated hesperetin lipid nanoparticles are sequentially constructed. The series of nanoparticles have the characteristics of uniform particle size distribution, high encapsulation efficiency, good stability and the like, and show a remarkable'gastric stagnation intestinal release 'intelligent switching effect in simulated gastrointestinal fluid. The delivery system can significantly improve the dissolution rate and bioavailability of hesperetin, and provides an effective nanotechnology strategy for solving the problems of poor water solubility, low oral absorption rate and the like of hesperetin.