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Method for preparing naringenin, hesperetin and mono-glucoside mixtures of naringenin and hesperetin

A technology for hesperetin and naringenin, which is applied in directions such as pharmaceutical combinations, pharmaceutical formulations, and medical preparations containing active ingredients, can solve the problem that there is no literature report on the preparation method of plant extracts, it is difficult to obtain extracts, and there is no discovery. problems, to achieve the effect of saving the use of organic solvents, high yield and short time consumption

Inactive Publication Date: 2018-01-19
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are no reports of plants or traditional Chinese medicines containing naringenin, naringenin monoglycoside, hesperetin, and hesperetin monoglycoside at the same time
[0009] Although the above-mentioned plants or Chinese medicines contain 1 or 2 or 3 kinds of naringenin, naringenin monoglycoside, hesperetin, and hesperetin monoglycoside, their content is very low, and it is difficult to obtain these ingredients as The extracts of the main components are used in the research and development or production of corresponding food, health food and new traditional Chinese medicine
[0010] So far, no plant containing naringenin monoglycoside, hesperetin monoglycoside, naringenin and hesperetin has been found, and there is no literature report that simultaneously contains naringenin monoglycoside, hesperetin monoglycoside, naringenin and hesperetin. Two or three or four plant extracts containing hesperetin and preparation method thereof

Method used

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  • Method for preparing naringenin, hesperetin and mono-glucoside mixtures of naringenin and hesperetin
  • Method for preparing naringenin, hesperetin and mono-glucoside mixtures of naringenin and hesperetin
  • Method for preparing naringenin, hesperetin and mono-glucoside mixtures of naringenin and hesperetin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Take 10g of Citrus aurantium, crush, sieve, dissolve in 200mL of 85% ethanol, add hydrochloric acid to make the concentration reach 1.6mol / L, stir in a water bath at a temperature of 75°C, extract and hydrolyze, react for 2.5h; recover ethanol, Obtained 4.8 g of a brown solid. As determined by HPLC, the contents of the four compounds in the mixture are 6.3% naringenin monoglycoside, 4.1% hesperetin monoglycoside, 3.9% naringenin and 2.4% hesperetin.

Embodiment 2

[0039] Take 10g of Citrus aurantium, crush, sieve, dissolve in 200mL of 85% ethanol, add hydrochloric acid to make the concentration reach 1.6mol / L, stir in a water bath at a temperature of 75°C, extract and hydrolyze, react for 2.5h; recover ethanol, Extract with ethyl acetate (200mL×4), recover ethyl acetate under reduced pressure to obtain 3.49g of a yellow solid, as determined by HPLC, the contents of the four compounds in this mixture are respectively 13% of naringenin monoglycoside, 13% of hesperetin monoglycoside 11%, naringenin 7%, hesperetin 5%. The conversion rate of prunin was 46.0%; the conversion rate of hesperetin monoglycoside was 39.0%; the conversion rate of naringenin was 40.1%; the conversion rate of hesperetin was 26.3%.

Embodiment 3

[0041] Take 10g of Citrus aurantium, crush, sieve, dissolve in 200mL of 85% ethanol, add hydrochloric acid to make the concentration reach 1.6mol / L, stir in a water bath at a temperature of 75°C, extract and hydrolyze, react for 2.5h; recover ethanol, Extract with ethyl acetate (200mL×4), recover ethyl acetate under reduced pressure to obtain 3.40g of yellow solid, take this mixture as mobile phase and carry out silica gel column chromatography, collect naringenin, naringenin monoglycoside, hesperetin 1. The components of hesperetin monoglycoside were combined, and the solvent was recovered under reduced pressure to obtain 2.4 grams of the plant extract with the highest content. As determined by HPLC, the contents of the four compounds in the mixture are 18.0% of naringenin monoglycoside, 15.4% of hesperetin monoglycoside, 10.2% of naringenin and 7.6% of hesperetin.

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Abstract

The invention provides a medicinal composition mainly containing naringenin, Prunin, hesperetin and hesperetin-7-0-glucoside. A medicine containing naringin, narirutin, hesperidin and neohesperidin isutilized as a raw material, an extract of the medicine is hydrolyzed, or extracting and hydrolyzing are conducted simultaneously on the extract of the medicine, and the plant extract can be obtained.The medicinal composition can be used for development and production of foods, health foods and new traditional Chinese medicines.

Description

technical field [0001] The invention provides a brand-new Chinese medicine (or plant) extract and a preparation method thereof, in particular to a brand-new plant extract mainly containing naringenin, naringenin monoglycoside, hesperetin, hesperetin monoglycoside A compound and a preparation method thereof belong to the research field of traditional Chinese medicine or natural medicine. Background technique [0002] Naringenin monoglycoside (Prunin), also known as naringenin-7-O-glucoside (Naringenin-7-O-glucoside), is a molecule that is hydrolyzed by rutin naringin (Narirutin) or naringin (Naringin) Dihydroflavone monoglycoside obtained from rhamnose, white solid, melting point 225°C, molecular formula is C 21 h 22 o 10 , the molecular weight is 434.39. Literature shows that naringenin monoglycoside has antibacterial, antioxidative, antiallergic, antiviral, lowering cholesterol, lowering blood sugar, inhibiting platelet aggregation, promoting periosteal cell proliferati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61K31/352A61P37/08
Inventor 金永日刘伟杰李绪文
Owner JILIN UNIV
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