95 results about "Neohesperidin" patented technology

The invention discloses an application of isorhamnetin-3-O-neohesperidin in preparation of a medicine for preventing and / or treating benign prostatic hyperplasia. In-vitro experiments show that the isorhamnetin-3-O-neohesperidin can inhibit proliferation of prostatic hyperplasia cells, promote apoptosis of the prostatic hyperplasia cells and regulate inflammatory response of the prostatic hyperplasia cells, and rat experiments show that the isorhamnetin-3-O-neohesperidin can improve the hormone endocrine disorder condition of an animal model body, and can inhibit the prostatic hyperplasia cells and promote the prostatic hyperplasia cell apoptosis and regulate the inflammatory response of the prostatic hyperplasia cells. The anti-inflammatory ability of an animal model is enhanced, and the traditional Chinese medicine composition has a relatively good treatment effect on prostatic hyperplasia caused by testosterone propionate. The medicine for preventing and / or treating benign prostatic hyperplasia, provided by the invention, contains a pharmacodynamic dose of isorhamnetin-3-O-neohesperidin and a pharmaceutically acceptable carrier or auxiliary material, can be prepared into a medicine dosage form which is convenient for clinical use and can play an optimal curative effect, and has a relatively high clinical application value.

The invention discloses a high-power compound plant sweetener and a preparation method thereof, and belongs to the technical field of food chemistry. Every 100 g of the high-power compound plant sweetening agent is prepared from the following components: 0.001 to 5 g of mogroside, 0.001 to 2 g of stevioside, 0.001 to 5 g of liquorice root extract, 0.0001 to 2 g of neohesperidin dihydrochalcone, 0.01 to 0.015 g of stachyose, 0.005 to 0.01 g of D-erythorbic acid, 0.005 to 0.01 g of ascorbic acid, and the balance of water-soluble dietary fiber. The pure plant high-power sweetener which is safe and healthy, has low energy and low carbohydrate and is rich in dietary fiber and protein is prepared, has obvious functions of healthy food, and is low in energy and less in carbohydrate and contains a certain amount of protein compared with cane sugar under the same condition.

The invention discloses a composition and application thereof, and the composition comprises synephrine or a pharmaceutically acceptable salt thereof, and further comprises naringin, neohesperidin and / or hesperidin. The composition prepared by compounding the synephrine or the pharmaceutically acceptable salt thereof with the naringin, the neohesperidin and / or the hesperidin has the effects of relieving, repairing, resisting wrinkles, easing pain, relieving itching or resisting allergy when being used as a functional component of a skin care product; when being used as a functional component of a scalp care product, the shampoo has the effects of removing dandruff, removing oil, relieving itching or preventing alopecia.

The invention relates to the technical field of quality detection, and discloses a method for determining the content of five components of liver-soothing tablets based on a double-label multi-measurement method, and the method comprises the following steps: S1, extracting a test solution; s2, preparing a reference substance solution; s3, preparing each negative control solution; s4, determining chromatographic conditions; s5, constructing a retention time prediction model by using a double-marking linear correction method, taking the standard retention time of paeoniflorin and hesperidin as a horizontal coordinate, taking the actual retention time of paeoniflorin and hesperidin in the chromatographic column as a vertical coordinate, and substituting the standard retention time of agarotetrol, naringin and neohesperidin into the equation to calculate and obtain predicted retention time respectively; s6, determining the contents of paeoniflorin, agarotetrol, naringin, hesperidin and neohesperidin in the liver soothing tablet sample by adopting a double-label multi-determination method; according to the method, the contents of five components, namely paeoniflorin, agarotetrol, naringin, hesperidin and neohesperidin, in the liver-soothing tablet are simultaneously determined by a double-label multi-measurement method, the accuracy is high, and the cost is low.

The present invention relates to the technical field of detection of Chinese medicine formula granules, and discloses a detection method for orange and citrus aurantium formula granules. The method comprises first comparing the correspondence of characteristic peaks in the chromatograms of the test solution and the control medicinal material solution, and then further detecting the citrus aurantium formula granules according to the peak area ratio α1 of rutin naringin to naringin and the peak area ratio α2 of neohesperidin to hesperidin. The UPLC detection method has good accuracy, repeatability and separation, shortens the detection time, and is easy to operate. The present invention uses the simulated formula granule extraction solution as the evaluation benchmark, can accurately detect whether the formula granules are orange and citrus aurantium formula granules, makes up for the vacancy in the technical field of its origin identification, and the method can be used as an exclusive identification method for orange and citrus aurantium formula granules.

The invention belongs to the technical field of traditional Chinese medicines, and discloses an immature bitter orange-derived extracellular vesicle-like nanoparticle, which is characterized in that the immature bitter orange-derived extracellular vesicle-like nanoparticle is prepared by the steps of crushing immature bitter orange, filtering, centrifuging and resuspending, the average particle size is 60-120 nm, and the particle size of the immature bitter orange-derived extracellular vesicle-like nanoparticle is 20-30 nm. The extracellular vesicle-like nanoparticles are used for entrapment of at least one of naringin, neohesperidin, naringenin and hesperidin. The extracellular vesicle-like nanoparticles derived from immature bitter oranges are simple in preparation method, have liver targeting and good stability, and can be used for preparing medicines for preventing and / or treating liver injury, depression and breast cancer.

The invention belongs to the technical field of quality detection of traditional Chinese medicines, and discloses a quality detection method of rhizoma atractylodis macrocephalae fried with fructus aurantii, which is characterized by comprising the following steps: performing qualitative analysis on rhizoma atractylodis macrocephalae fried with fructus aurantii by respectively adopting a thin-layer chromatography and an HPLC (High Performance Liquid Chromatography) characteristic chromatogram; a high performance liquid phase external standard curve method is adopted to carry out quantitative analysis on atractylenolide I, atractylenolide II, atractylenolide III, naringin and neohesperidin in the fructus aurantii fried bighead atractylodes rhizome. The invention establishes a thin-layer identification method for rhizoma atractylodis macrocephalae and fructus aurantii in a fructus aurantii fried rhizoma atractylodis macrocephalae processed product; according to the method for establishing the fingerprint spectrum, the similarity of 10 batches of rhizoma atractylodis macrocephalae fried with fructus aurantii ranges from 0.991 to 1.000; the method for determining the content of atractylenolide I, atractylenolide II, atractylenolide III, naringin and neohesperidin is established, scientific support is provided for formulating the quality standard of the characteristic processing decoction pieces of the temporary prescription, and effective guarantee is provided for clinical application of the decoction pieces.

The invention relates to a sweetening composition comprising a naturally sweet carbohydrate, a stevia compound, rubusoside, and tannin, wherein component d) is present in an amount on the scale of 1 mg / l to 400 mg / l; the invention also relates to a sweetening composition comprising a naturally sweet carbohydrate, a stevia compound, rubusoside, tannin, and oak extract or pomegranate extract; the invention further relates to a sweetening composition comprising acesulfame or aspartame, sucralose, a stevia compound, saccharin or cyclamate, neohesperidin, and tannin in an amount on the scale of 1 mg / l to 400 mg / l; furthermore, the invention relates to a sweetening composition comprising a) 0.1 to 50 g / l of a naturally sweet-tasting carbohydrate, b) 40 to 120 g / l of a naturally sweet-tasting carbohydrate other than a), and c) 30 to 300 mg / l of rubusoside; and, the invention relates to the use of said sweetening compositions for sweetening and preparing beverages.

The invention relates to a construction method of a fingerprint spectrum of Shenxiong Tongmai capsule, which comprises the following steps: dissolving a ferulic acid standard substance, a hesperidin standard substance, a ginsenoside Re standard substance, a neohesperidin standard substance and a naringin standard substance in methanol to prepare a mixed reference substance solution; the method comprises the following steps: respectively dissolving contents of Shenxiong Tongmai capsules in a plurality of batches in a methanol aqueous solution to prepare a plurality of test solutions; respectively carrying out high performance liquid chromatography detection on the mixed reference substance solution and the plurality of test sample solutions to obtain a high performance liquid chromatogram of the mixed reference substance solution and high performance liquid chromatograms of the plurality of test sample solutions; determining characteristic peaks and identifying chemical components of the characteristic peaks, and calculating relative retention time of other characteristic peaks by taking the characteristic peak corresponding to hesperidin as a reference peak to obtain the fingerprint spectrum. The fingerprint spectrum obtained by the construction method disclosed by the invention can well reflect information of a plurality of index components of the Shenxiong Tongmai capsule.

This invention relates to a highly active effective fraction of the Epiglottis-Clearing Decoction and its application in the preparation of antitussive drug compositions, belonging to the field of traditional Chinese medicine extract technology. The effective fraction contains hydroxysaffron yellow A, paeoniflorin, naringin, and neohesperidin, with the total content of these four components not less than 20% of the total mass of the effective fraction. The effective fraction of this invention is prepared by purification with macroporous resin. Compared with the freeze-dried powder of the traditional Epiglottis-Clearing Decoction, the total content of the four active ingredients is increased from 3.32% to 20.87%, achieving a more than 6-fold high-efficiency enrichment. Animal pharmacodynamic experiments show that the antitussive effect of a low dose (275.5 mg / kg) of this effective fraction is comparable to that of the freeze-dried powder of the original decoction (1928 mg / kg), reducing the dosage to about 1 / 7 of the original formula, demonstrating a significant "synergistic effect with reduced dosage" technical effect. The effective active ingredients provided by this invention have a clear content and stable quality, and can be made into modern preparations such as tablets, capsules or granules, solving the problems of large volume, inaccurate dosage and poor patient compliance of traditional decoctions.

The invention discloses a heart-protecting granule identification and quality control method based on thin layer chromatography, and belongs to the technical field of medicine detection. The thin-layer chromatography identification method comprises the following steps: identifying astragalus membranaceus by taking astragaloside as a reference substance, identifying wine-treated rhubarb by taking chrysophanol and emodin as reference substances, identifying leech by taking a leech medicinal material as a reference substance, identifying liquorice by taking liquiritin and a liquorice medicinal material as reference substances, and identifying radix rehmanniae by taking catalpol as a reference substance. And identifying salvia miltiorrhiza by taking salvianolic acid B and salvia miltiorrhiza medicinal materials as reference substances, and identifying fructus aurantii by taking naringin and neohesperidin as reference substances. By establishing the thin-layer chromatography identification method of the heart-protecting granules, an exclusive developing solvent and a developing mode are adopted, accurate identification is achieved according to the characteristics of spot colors, fluorescence characteristics and the like of corresponding positions of a test substance chromatography, a reference substance and a reference medicinal material chromatography, and the method is easy and convenient to operate, low in detection cost and high in repeatability and specificity; and quality control can be effectively carried out in the production process of the heart-protecting granules.

The invention relates to a traditional Chinese medicine preparation for treating gastritis and a preparation method of the traditional Chinese medicine preparation. The traditional Chinese medicine preparation comprises the following raw materials in parts by weight: 40-50 parts of a traditional Chinese medicine composition, 10-15 parts of a sustained release agent, 25-32 parts of a filling agent and 3-8 parts of a flavoring agent, the slow release agent is modified carboxymethyl konjac glucomannan; the filling agent is cordyceps militaris polysaccharide and lactose; the flavoring agent is aspartame or sucralose. The sustained release agent is neohesperidin dihydrochalcone modified carboxymethyl konjac glucomannan, neohesperidin dihydrochalcone has the effect of inhibiting gastric acid secretion, and neohesperidin dihydrochalcone and the traditional Chinese medicine ingredient cuttlebone can synergistically play a role in preparing acid and jointly play a role in protecting gastric mucosa. Meanwhile, the cordyceps militaris polysaccharide has polysaccharide generality of thickening and stable film forming, and forms a compact network structure together with the modified carboxymethyl konjac glucomannan, and the obtained traditional Chinese medicine preparation has a good slow release effect.

The present invention relates to a method for preparing a new eriocin. The method solves the technical problems of the existing method for preparing new eriocin, such as low yield, complicated operation, long time consumption, and being unfavorable for industrial production. The method for preparing a new eriocin of the present invention comprises the following steps: 1) uniformly mixing neohesperidin with an alkaline solution in proportion, heating and performing a hydrolysis and ring-opening reaction, and cooling to room temperature after the reaction is completed; adjusting the pH to neutral, cooling, filtering, and drying in sequence to obtain an intermediate; 2) mixing the intermediate with protocatechuic aldehyde in proportion, dissolving in an organic solvent, adding aniline catalyst, heating and performing a condensation reaction under nitrogen protection, reacting until the intermediate content is less than 0.5%, and stopping the reaction; 3) recovering the solvent under reduced pressure until the solvent is near zero, washing and filtering to obtain a crude new eriocin; and 4) refining with ethanol to obtain a refined new eriocin. The method of the present invention can quickly and efficiently prepare a high-purity refined new eriocin.

The invention provides a pericarpium citri reticulatae fingerprint spectrum, a construction method and application thereof, a multi-index component detection method of pericarpium citri reticulatae, an identification standard of pericarpium citri reticulatae varieties and a construction method of the identification standard, and relates to the technical field of analysis and detection. The pericarpium citri reticulatae fingerprint spectrum provided by the invention has 17 characteristic peaks, wherein the 17 characteristic peaks comprise the characteristic peaks of narirutin, hesperidin, neohesperidin, sweet orange flavone, nobiletin, 5-demethylnobiletin, hesperetin and heptamethoxyflavone. According to the pericarpium citri reticulatae fingerprint spectrum provided by the invention, a plurality of substances are used as quality indexes of the pericarpium citri reticulatae, so that accurate and comprehensive quality control on the quality of the pericarpium citri reticulatae can be realized, and an excessively simple currently executed quality standard of the pericarpium citri reticulatae is perfected. According to the method, the mass ratio of narirutin to hesperidin to neohesperidin to sweet orange flavone to nobiletin to 5-demethylnobiletin is taken as an identification standard of the dried orange peel variety, and accurate identification of six different dried orange peels including Citrus chachiensis, Dahongpao tea, Satsuma orange, Fujian orange, local early orange and Nanfeng orange can be realized.

The present invention relates to a method for establishing a characteristic chromatogram of Xiao'er Fengre Qing mixture, comprising the following steps: (1) Preparation of a reference substance solution: Weigh appropriate amounts of chlorogenic acid reference substance, paeoniflorin reference substance, baicalin reference substance, phillyrin reference substance, arctiin reference substance, naringin reference substance, and neohesperidin reference substance, and prepare a solution; (2) Preparation of a test sample solution: Pipette Xiao'er Fengre Qing mixture, dilute, shake well, and filter to obtain the solution; (3) Determination: Use high performance liquid chromatography to separately detect the test sample solution and the reference substance solution, obtain the characteristic chromatogram of the test sample solution and the characteristic chromatogram of the reference substance solution, conduct qualitative analysis by comparing retention times, and conduct attribution and positioning of the target components, thereby obtaining the characteristic chromatogram of the pharmaceutical preparation.

The invention discloses application of neohesperidin and limonin as markers for identifying Hu pomelo honey. The application comprises the following steps: measuring the content of neohesperidin and limonin in a honey sample to be detected; if the content of neohesperidin in the honey sample to be detected is greater than 0.14 mg / kg and the content of limonin is greater than 0.13 mg / kg, judging that the honey sample to be detected is the Huyou honey. According to the invention, neohesperidin and limonin are adopted as markers for identifying the Hu pomelo honey, a corresponding detection method is established, the Hu pomelo honey can be accurately distinguished from other honey by adopting the detection method, and the method has an application prospect in the aspects of identification, authenticity evaluation and the like of the Hu pomelo honey.

The invention discloses a composition with a whitening effect. The composition is prepared from the following components in parts by mass: 47 to 51 parts of grape seed oil, 47 to 51 parts of pomegranate seed oil, 0.5 to 0.8 part of 4-butylresorcinol, 0.2 to 0.6 part of VC-IP (Vitamin C-IP) and 0.1 to 10 parts of isorhamnetin-3-O-neohesperidin. The invention also discloses a preparation method of the composition. The invention also discloses an essence oil containing the composition. The invention also discloses a face cream containing the composition. The invention also discloses application of the composition in preparation of cosmetics for skin whitening, spot fading and skin yellowing reduction. The composition provided by the invention establishes a multi-target synergistic pathway: 1, a blackness removal pathway for reducing the activation of oxidative stress such as ultraviolet rays on melanocytes; and 2, a jaundice removal pathway: inhibiting lipid peroxidation and promoting lipofuscin removal.

The invention discloses a multi-index content determination method of hovenia acerba and rhizoma atractylodis bowel-relaxing granules, and belongs to the technical field of traditional Chinese medicine detection. The invention establishes a high performance liquid chromatography method for simultaneously determining nine components including caffeic acid, chicoric acid, naringin, naringin, hesperidin, neohesperidin, chrysophanol, aurantio-obtusin and atractylenolide I in a preparation, and the content of the components is calculated through a relative correction factor by taking caffeic acid as an internal reference by adopting a quantitative analysis of multi-components by single marker. According to the method, effective detection of the immature bitter orange medicinal material and the immature bitter orange base source is realized, and the immature bitter orange and the sweet orange base source of the traditional Chinese medicine immature bitter orange can be accurately distinguished, so that more comprehensive and accurate quality control of the traditional Chinese medicine is realized, and the stability and consistency of clinical efficacy of the traditional Chinese medicine are guaranteed.

The invention discloses natural neohesperidin and a separation and purification method thereof, and belongs to the technical field of natural product extraction.The high-purity neohesperidin is prepared from immature bitter oranges through smashing, extraction, coarse crystallization, organic solvent pulping, water pulping and recrystallization. The problems of complex process, long production period, high equipment requirement, high comprehensive production cost, high product ash content and the like in the existing neohesperidin separation and purification process are solved.

The invention discloses saccharomyces cerevisiae and a method for efficiently synthesizing neohesperidin by using cane sugar. According to the saccharomyces cerevisiae, on the basis of ZQH01, FjTAL derived from Flavobacterium johnsoniae, AtC4H, AtPAL2 and AtATR2 derived from Arabidopsis thaliana and CYB5 derived from yeast are inserted into a genome of the ZQH01, and the saccharomyces cerevisiae is prepared by the following steps of: adding the FjTAL, the AtC4H, the AtPAL2 and the AtATR2 into the genome of the ZQH01; according to the invention, a sucrose phospholysis double-carbon-source system is introduced into the saccharomyces cerevisiae, sucrose can be directly converted into fructose and glucose-1-phosphoric acid, the fructose is specially used for cell growth and hesperetin synthesis, the glucose-1-phosphoric acid directionally supplies UDP-glucose, and a carbon flow is physically separated to eliminate flux competition caused by a Crab effect. The saccharomyces cerevisiae disclosed by the invention can be used for efficiently synthesizing neohesperidin by using cane sugar, and has important significance on green and efficient acquisition of citrus flavonoids.

This invention discloses a method for preparing a new hesperidin, belonging to the field of organic chemical synthesis technology. The method has simple reaction conditions, is safe and environmentally friendly, and can obtain high-purity new hesperidin without purification after the reaction. The method includes the following steps: (1) Take root bark acetylphenyl-4'-β-neohesperidin, add n-butanol, isovalin and 3-indoleacetic acid, and heat to react; (2) After the reaction solution in step (1) has reacted completely, cool down; (3) Filter the cooled solution, wash the solid with n-butanol, and dry to obtain the finished product.

The invention is applicable to the technical field of biological medicine, and provides application of isorhamnetin-3-O-neohesperidin in preparation of a medicine for treating alopecia. Research finds that isorhamnetin-3-O-neohesperidin can promote hair growth and protect hair follicle stem cells, and as a natural extract, the isorhamnetin-3-O-neohesperidin can be used for preparing a medicine for treating alopecia. The traditional Chinese medicine composition has the advantages of being low in cost, few in side effect, good in patient compliance, easy to obtain and the like, and a new thought is provided for preparing the medicine for treating alopecia, especially the medicine for treating androgenetic alopecia.

The present invention belongs to the technical field of enzyme engineering, and particularly relates to a sucrose synthase mutant and its application. The sucrose synthase mutant described in the present invention is obtained by the following mutations on the amino acid sequence of sucrose synthase shown in SEQ ID NO.1: valine at position 159 is mutated to threonine. The sucrose synthase mutant has high enzyme activity and can be applied to the biocatalytic synthesis of neohesperidin. The present invention also provides a method for preparing neohesperidin using the sucrose synthase mutant. Using hesperetin-7-O-glucoside, sucrose, and uridine diphosphate as substrates, and adding the whole-cell crude enzyme solution of rhamnosyltransferase, rhamnose synthase, and sucrose synthase, neohesperidin is catalytically obtained with a relatively high conversion rate.

The invention discloses application of oxidized berberine and neohesperidin in improving intestinal health of broiler chickens, and belongs to the technical field of feed additives. Research finds that oxidized berberine and neohesperidin can increase the intestinal villus height, crypt depth and goblet cell number of broiler chickens and improve intestinal health; the slaughter indexes such as daily weight gain and feed intake can be improved, the feed-weight ratio is reduced, and the slaughter indexes such as carcass weight and semi-eviscerating weight are increased; meanwhile, the serum immune factor and antioxidant index level is improved, the safety is high, and no obvious toxicity exists; and the two components are combined for use, so that a synergistic interaction effect is also achieved. The invention provides a new raw material resource for improving the intestinal health of the broiler chicken and improving the production performance and economic value of the broiler chicken.

The invention relates to a Jichuan decoction composition QAMS (quantitative analysis of multi-components by single marker) content detection method which comprises the following steps: extracting a Jichuan decoction composition by using an extraction solvent to prepare a test solution; dissolving the reference substance and the naringin by using a dissolving reagent to prepare a reference substance mixed solution and a naringin solution; respectively injecting the test solution, the reference substance mixed solution and the naringin solution, and carrying out ultra-high performance liquid chromatography detection; and by taking naringin as an internal reference substance, calculating the content of the component to be detected in the test solution according to a formula. According to the method, the content of eight components including caffeic acid, echinacoside, ferulic acid, isoferulic acid, verbascoside, naringin, neohesperidin and chrysoidine in the Jichuan decoction composition is obtained by adopting a quantitative analysis of multi-components by single marker with naringin as an internal reference substance, the practicability is high, the operation is simple, the test is rapid and accurate, the cost is saved, the operation is simple and convenient, and the method is suitable for industrial production. And a basis is provided for establishing quality evaluation of the Jichuan decoction composition.

The invention discloses polygalacturonic acid (PGA) and application of a neohesperidin dihydrochalcone (NHDC) or neohesperidin (NH) embedded compound of the polygalacturonic acid (PGA) in preparation of anti-allergic food or drugs. The PGA has good biocompatibility and immune regulation and intestinal barrier repair functions. Experiments show that the PGA can significantly reduce IgE-mediated food anaphylaxis, alleviate allergic symptoms and improve intestinal structures. PGA is adopted as a carrier, and the PGA and NHDC / NH form non-covalent binding through intermolecular force to prepare the PGA-NHDC / PGA-NH compound, so that the solubility and the stability of the NHDC / NH are improved, and a synergistic anti-allergic effect is also generated. The PGA-NHDC / PGA-NH compound has the advantages of small molecular weight, fast absorption and non-immunogenicity, and can release NHDC / NH in colon in a targeted manner. On the basis, the invention further provides the application of the PGA-NHDC / PGA-NH compound in preparation of the orange juice, the obtained juice system is in a stable suspension state, has no precipitation layering phenomenon, has targeted synergistic antianaphylaxis and high storage stability, and remarkably improves the functionality and the storage stability of the juice.

The invention relates to a preparation method of a high-purity neohesperidin dihydrochalcone crystal, which comprises the following steps: dissolving neohesperidin with an alkaline solution, and adding acid and an alcohol solvent for crystallization and purification to obtain high-purity neohesperidin, the method comprises the following steps: reacting neohesperidin in the presence of an alkali solution, hydrogen, palladium on carbon or raney nickel to generate neohesperidin dihydrochalcone, adjusting the pH value, adding alcohol as a mixed solvent, and separating out at normal temperature to obtain high-purity neohesperidin dihydrochalcone; and recrystallizing with alcohol / methyl tertiary ether to obtain the neohesperidin dihydrochalcone crystal. According to the method disclosed by the invention, the problem of low purity of neohesperidin dihydrochalcone in the prior art is solved, and the obtained neohesperidin dihydrochalcone needle-shaped crystal is high in sweetness, long in sweet holding time, high in sweet abundance and long in residual sweet aftertaste.

The invention discloses an immature bitter orange polyphenol extract as well as an extraction method and an optimization method thereof, and relates to the technical field of plant extraction. The extraction method comprises the following steps: S1, taking immature bitter orange as a raw material, and performing ultrasonic-assisted extraction by using ethanol to obtain a crude extract; s2, adding the crude extract into water, heating to dissolve the crude extract, cooling, adding absolute ethyl alcohol, standing, and centrifuging to remove precipitate to obtain a concentrated solution; and S3, carrying out dynamic adsorption and desorption on the concentrated solution by using resin, eluting by using ethanol, collecting the eluent, and concentrating to obtain the product. According to the method, parameters such as ethanol volume fraction, liquid-solid ratio, ultrasonic time, power and temperature are optimized through a response surface analysis method, the most suitable resin is screened out for further purification, the method has the advantages of high yield, low cost and environmental protection, efficient and green extraction preparation of the total phenols of the immature bitter oranges is achieved, the naringin content in the extract is higher than 50 g / 100 g, and the method is suitable for industrial production. The content of neohesperidin is higher than 10g / 100g.

The invention discloses application of a recombinant plasmid, a recombinant strain and a preparation in improvement of methyltransferase activity and production of neohesperidin, and belongs to the technical field of bioengineering. The invention aims to solve the technical problems of insufficient YLOMT activity, high eriodictyol residual quantity, low synthesis efficiency, low yield and the like when the yarrowia lipolytica is used for synthesizing neohesperidin in the prior art. The key point of the technical scheme is that a recombinant plasmid is provided, the recombinant plasmid comprises a fusion fragment, and the fusion fragment comprises a coding sequence of a mitochondrial localization signal peptide, a Kozak sequence and a YLOMT coding gene; the amino acid sequence of the mitochondrial positioning signal peptide is shown as SEQ ID NO. 36; the nucleotide sequence of the Kozak sequence is as shown in SEQ ID NO. 37; and the sequence of the YLOMT coding gene is as shown in SEQ ID NO. 38.

The present invention discloses a method for determining the contents of six components in Qingwei Bao'an Pills by the method of multi-component quantification by single marker, belonging to the technical field of traditional Chinese medicine detection. The method for determining the contents of six components in Qingwei Bao'an Pills by the method of multi-component quantification by single marker provided by the present invention uses neohesperidin as an internal reference substance. According to the relative correction factors obtained between neohesperidin and liquiritin, narirutin, naringin, hesperidin, and ammonium glycyrrhizinate, the contents of six main pharmacologically active components in Qingwei Bao'an Pills can be quickly detected, the detection efficiency can be improved, a basis is provided for the quality control standard of Qingwei Bao'an Pills products, and it is convenient for the production and detection of Qingwei Bao'an Pills.