216 results about "Neohesperidin" patented technology

The invention discloses a total chromocor extract from traditional medicine citrus fruit and preparing method, which is characterized by the following: comprising pomelo ped glycosides, new orange peel glycosides, orange peel glycosides, pomelo ped rutin, derivant and so on; choosing one or several methods from solvent extraction, solvent extraction process, macroreticular absorption resin method, lead salt precipitation method, column chromatography, supercritical fluid chromatography, liquid-liquid counter-current partition chromatography and so on; producing extract; setting percentage content of various chromocor element at 5-100%(w / w) in the extract; counting 5-100%(w / w) of pomelo ped glycosides, new orange peel glycosides, orange peel glycosides in total chromocor content.

The invention provides a sweet taste functional clustering substance preparation which has the taste close to tobacco sweet taste and is capable of obviously increasing the tobacco taste, which comprises the following compositions in mass percentage: 0.0001 to 0.0004 percent of clove oil, 0.0001 to 0.0006 percent of anise oil, 0.2 to 0.8 percent of date oil, 0.01 to 0.03 percent of maltol, 0.05 to 0.15 percent of dihydroactinidiolide, 0.002 to 0.008 percent of benzyl cinnamate, 0.1 to 0.6 percent of gamma-Valerolactone, 0.002 to 0.008 percent of phenethyl acetate, 0.0001 to 0.0003 percent of menthyl acetate, 0.0001 to 0.0006 percent of Beta-Damascenone, 0.02 to 0.08 percent of methyl cyclopentyl hydroxybenzyl thiocyanide ketone, 0.003 to 0.009 percent of dihydrogen Beta-Damascenone ketene, 0.001 to 0.003 percent of raspberry ketone, 0.01 to 0.05 percent of neohesperidin, 0.03 to 0.09 percent of vanilline, 0.001 to 0.005 percent of perillartine, 0.01 to 0.03 percent of aspartame, 0.02 to 0.08 percent of glycyrrhizine, 0.01 to 0.03 percent of malic acid, 0.002 to 0.008 percent of 2- methylbutanoic acid, 0.02 to 0.08 percent of sodium glutamate, 0.01 to 0.05 percent of sodium chloride, 0.002 to 0.006 percent of 2-phenylethanol, 0.0001 to 0.0003 percent of geraniol, 0.002 to 0.008 percent of nerolidol, 0.5 to 1.5 percent of guarana extractive, 1.0 to 2.0 percent of tomato extractive, 0.1 to 0.3 percent of grape extractive, and 95 percent of ethanol residue. The addition of the spice preparation can greatly improve the tobacco quality of cigarettes, and increase rich and inviting sweet taste of the cigarettes, thereby reducing the use level of sound tobacco. Moreover, the product has stable quality and good economic benefits.

The invention discloses a method for separating high-purity hesperidin, neohesperidin, naringin and synephrine from fructus aurantii Immaturus, and relates to a method for extraction of effective components of traditional Chinese medicines. The method includes the following steps: (1) enzymolysis, (2) extraction, (3) membrane separation, (4) synephrine separation, (5) naringin separation, (6) neohesperidin separation, and (7) hesperidin separation. The method has the advantages that the method overcomes the shortcomings in prior art, provides a new effective method which is easy to industrially produce, and is capable of combined extraction and separation various effective components in the fructus aurantii Immaturus, the method has the advantages of simple operation, high extraction rate, and high purity of the effective components, and the effective components all are separated, and easy to use.

The invention discloses a method for preparing neohesperidin by an enzymic method, comprising the following steps: performing coordination between hesperidin and metal ions and dissolving in water, and realizing conversion from hesperidin to neohesperidin under the action of immobilized rhamnosidase and immobilized rhamnoside transferase. The method provided by the invention is adopted to solve the problem that production cost of neohesperidin widely used in medical, functional food and its cosmetic fields is high, and simultaneously opens up a new approach for recycling of industrial waste orange peel and high value-added utilization of hesperidin.

The invention relates to a solid, low calorie, palatable sugar substitute with the following ingredients, by weight: inulin 21.6%-48.3%; polydextrose 21.6%-59.0%; maltodextrin 2.0%-48.0%; trace amounts of an antiagglomerant, and the balance being one or more intense sweeteners, for example, sucralose or acesulfame-K or a combination of acesulfame-K and neohesperidin dihydrochalone. A solid, low calorie, chocolate comestible is provided as follows: inulin 12.0-20.0%; polydextrose 15.0-25.0%; maltodextrin 2.0-8.0%; milk 0-22% dependent upon flavor (dark, milk, etc.); cocoa mass 10-50% dependent upon flavor; cocoa butter 8-25% dependent upon flavor; trace amounts of lecithin and flavor; and the balance being one or more intense sweeteners such as aspartame, acesulfame-K or sucralose or a combination of acesulfame-K and neohesperidin dihydrochalone.

A neohesperidine is prepared from such vegetable Chinese-medicinal materials as bitter orange, ash bark, tangerine peel, evodia fruit, etc through extracting in alcohol, adsorbing by macroreticular resin column, separating with silica gel column, eluting, concentrating, and repeated recrystallizing. It can be used in quality control.

The invention relates to a novel function and application of seville orange flower extract. Particularly, hesperidin, neohesperidin and other flavonoid substances in the seville orange flower extract are capable of promoting cell proliferation and expression of mRNA of fibrocyte IV-type collagen, inhibiting activities of elastase and matrix metalloproteinase, reducing damage of ultraviolet ray to cells and reducing the level of reactive oxygen species and secretion of inflammatory factors, so that the seville orange flower extract has a function of resisting radiation and aging for skins. Therefore, the invention provides a new direction of seville orange flower extract application, namely application of the seville orange flower extract in skin protecting products.

The invention discloses a method for extracting high-purity neohesperidin from an immature bitter orange. The method comprises the following steps: (1) crushing and impregnating an immature bitter orange raw material in water, adding a compound plant-hydrolase preparation, and performing enzymatic decomposition to obtain an enzymatically decomposed immature bitter orange; (2) performing heating extraction, cooling, centrifugation and filtration on the enzymatically decomposed immature bitter orange to obtain enzymatic immature bitter orange extract; (3) performing water bath heat preservation on the enzymatic immature bitter orange extract, adding a flocculating agent for flocculation treatment, and performing centrifugation and filtration to obtain immature bitter orange flocculation liquid; (4) performing nanofiltration, concentration and vacuum drying on the immature bitter orange flocculation liquid to obtain a neohesperidin crude product; (5) completely dissolving the neohesperidin crude product in an ethanol solution, performing strict filtration, and repeatedly performing cooling crystallization, washing and vacuum drying to obtain a high-purity neohesperidin product. The purity of the product obtained by the method can reach 98.6 percent, and the final yield is more than 90 percent; the method is simple in process, high in operability, low in energy consumption and cost, suitable for industrial production, small in ethanol residue amount, environment-friendly and safe.

The invention relates to a synthesis method for neohesperidin dihydrochalcone. According to the synthesis method disclosed by the invention, neohesperidin dihydrochalcone is mainly prepared by carrying out hydrogenation on neohesperidin in an alkaline solution and hydrogen environment. The method is simple and easy to operate, low in environmental pollution, low in production, good in quality, high in yield, and suitable for industrialized production.

The invention belongs to the field of processing of grapefruits, and discloses pure natural honey grapefruit tea jam dense pulp and a preparation method thereof. The pure natural honey grapefruit tea jam dense pulp is prepared by preferably selecting the following raw materials, by mass, 10% of grapefruit peel, 50% of grapefruit pulp, 17% of rock candy, 10% of honey, 0.3% of grapefruit flowers, 0.5% of arabian jasmine flowers, 1% of honeysuckle, 1% of chrysanthemum, 0.2% of puer tea, 1% of green tea, 1% of black tea, 1% of rose, 1% of fructus lycii, 1% of lemon, 2% of red jujube with pits removed, 1% of hawthorn, 1% of fresh ginger and 1% of neohesperidin dihydrochlcone. L-cysteine in the pure natural honey grapefruit tea jam dense pulp is combined with vitamin C in grapefruit, when the pure natural honey grapefruit tea jam dense pulp is eaten frequently, the pure natural honey grapefruit tea jam dense pulp can clear heat, decrease internal heat, and whiten skin; naringin in grapefruit can lower the viscosity of blood, reduce formation of thrombosis, scatter body melanin in the body rapidly and relieve dark skin, and the edible value and therefore the nutritional effects of grapefruits can be improved.

The invention discloses a method for ultrasonic extracting and resin purifying sweetening agent in Lithocarpus polysachyus rehd leaf. The optimal ultrasonic extracting method is as follows: the ethanol concentration is 70%, the solid to liquid ratio is 1:25, and the ultrasonic time is 35min; the optimal AB-8 resin purifying process condition is as follows: the adsorption flow rate is 0.5mL / min, the elution ethanol concentration is 90%, the elution volume is 1.875BV, and the flow rate of the eluant is 1Ml / min. Under above extracting and purifying condition, the purity of the obtained phlorizin is 88.6164%, the purity of the neohesperidin dihydrochlcone is 71.8226%. The extracting and purifying method is simple and reliable and in favor of utilizing the sweetening agent in Lithocarpus polysachyus rehd leaf on scale.

The invention discloses a method for simultaneously determining twenty-two flavones and phenolic acids in citrus fruits by adopting high performance liquid chromatography-diode array detector-fluorescence detector (HPLC-DAD-FLD). The method is capable of simultaneously determining twenty-two phenolic compounds in citrus fruits such as gallic acid, synephrine, chlorogenic acid, protocatechuic acid,caffeic acid, p-coumaric acid, rhamnosylvitexin, eriocitrin, ferulic acid, rutin, benzoic acid, narirutin, naringin, hesperidin, diosmin, neohesperidin, quercetin, naringenin, kaempferol, nobiletin,hesperetin, acacetin and the like, derivatization is not needed, and the method is high in accuracy, high in sensitivity and excellent in repeatability.

The invention provides a method for simultaneously extracting synephrine and flavonoids from immature bitter oranges. The method comprises the steps as follows: the immature bitter oranges are smashed and extracted, acid is added to an extract for precipitation, and hesperidin is obtained; a precipitated filtrate is subjected to three steps of concentration including ultrafiltration membrane concentration and / or nanofiltration membrane concentration, vacuum decompression concentration and flue gas concentration, then a condensed extract is obtained, and is subjected to extraction, mixing with petroleum ether, crystallization and suction filtration, and then synephrine is obtained; the suction filtration liquid after suction filtration is condensed and mixed with ethanol, mixed liquor is formed and treated by macroporous adsorption resin, and neohesperidin and naringin are obtained. The ultrafiltration membrane concentration and / or nanofiltration membrane concentration and the flue gas concentration adopted by the method for simultaneously extracting synephrine and flavonoids from the immature bitter oranges can all reduce energy consumption, so that the solvent recovery rate is increased, and the filtrate treatment cost is lowered; differences between synephrine and other flavonoids in an extracting agent are used for separation and purification, therefore, the production process can be simplified, the production cost can be lowered, and the resource utilization rate can be increased.

The invention discloses a preparation method of neohesperidin dihydrochalcone, comprising the following steps of: (1) dissolving potassium hydroxide or sodium hydroxide in water with stirring so as to prepare 3-6% of an alkaline solution; (2) adding neohesperidin into the alkaline solution with stirring for dissolution, wherein the ratio of neohesperidin to the alkaline solution is 1: 5-8; (3) adding raneys nickel which accounts for 5-8 wt% the weight of neohesperidin after stirring and dissolving, carrying out a hydrogenation reaction at the hydrogen pressure of 0.01-0.1 MPa, stirring, and reacting for 4-6 hours to obtain a reaction solution; (4) adjusting the pH of the reaction solution to pH 7-7.5 by the use of hydrochloric acid, staying for 24-28 hours, and filtering out crystal solids to obtain the neohesperidin dihydrochalcone crude product. The preparation method disclosed in the invention requires no large pressure to carry out the hydrogenation reaction, is less dangerous, and is convenient for industrial production.

The high speed countercurrent chromatographic process of separating and purifying naringin, syinaringin, hesperidin and neohesperidin includes the step 1 of compounding solvent system comprising fixed phase and flowing phase and including ethyl acetate, fatty alcohol and water, filling the column of chromatograph with the fixed phase, rotating the column, pumping the flowing phase into the column, leading in sample via the valve and accepting the target component based on detection spectrogram; and the step 2 of high speed countercurrent chromatographic separating and purifying the obtained matter in the step 1, with the solvent system comprising chloroform, fatty alcohol and water. The said process is suitable for the separation and purification of one or more of naringin, syinaringin, hesperidin and neohesperidin in natural product or extract in high efficiency and is simple and feasible.

The invention relates to the field of organic synthesis and discloses a neohesperidin synthesis technology. The neohesperidin synthesis technology comprises the following steps of adding phloroacetophenone-4'-beta-neohesperidin and isovanillin (CAS: 621-59-0) as initial raw materials and a low-carbon alcohol solvent having the content of more than 98wt% into a three-necked flask, carrying out stirring for dissolution, feeding N2 into the three-necked flask to expel air in the three-necked flask, carrying out heating, orderly adding a main catalyst and a cocatalyst into the three-necked flask, carrying out timing at backflow starting time, stopping heating after the reaction lasts for 2-8h, carrying out cooling to a room temperature, carrying out filtration, washing the filter cake by a small amount of hot low-carbon alcohol, and carrying out vacuum drying at a temperature of 50 DEG C to obtain white solid neohesperidin powder. Compared with the existing neohesperidin synthesis technology, the neohesperidin synthesis technology provided by the invention has the advantages of low cost, high yield, high purity and small environmental pollution and is suitable for neohesperidin industrial production.

The invention provides a method for extracting neohesperidin from immature bitter orange and comprehensively utilizing immature bitter orange. The method for comprehensive utilization comprises the following steps: 1) crushing and sieving immature bitter orange; 2) leaching the sieved substance with water at temperature of 55-60 DEG C, and collecting the leachate and filter residue respectively; and 3) purifying the filter residue to obtain hesperidin; and / or separating the leachate to obtain one or more of pectin powder, naringin and neohesperidin. In the method provided by the invention, the immature bitter orange is utilized to the greatest extent; the method mainly adopts warm water for extraction and a little alcohol for separation and purification, and thus the use of organic solvent is minimized; and moreover, the technology is simple and effective and has a wide application value.

The invention relates to a quality control method for Turpinia arguta Seem leaf and a preparation containing the Turpinia arguta Seem leaves. The invention is the quality control method which applies gallic acid, apigenin-7-0-neohesperidin glycosidase, apigenin-7-0-2'-rhamnose rue glycosidase, apigenin-7-0- Beta -D-glucoside and apigenin, etc., as the indexes of the quality control. The method can effectively control the quality of the medicine and the preparation containing the medicine, so that the invention is positive in assuring the safety, effectiveness and controllable quality on the medicine.

The invention provides a near infrared transmitted spectrum detection method of naringin and / or neohesperidin. The near infrared transmitted spectrum detection method comprises the following steps of 1, adding alkaline water into traditional Chinese medicines containing naringin and / or neohesperidin, wherein the amount of the alkaline water is 5-15 time the amount of the traditional Chinese medicines, carrying out decoction to obtain a decoction, and carrying out filtration to obtain a liquid to be detected, 2, carrying out scanning of the liquid to be detected by a near-infrared spectrograph to obtain near-infrared spectroscopy data, and 3, determining naringin and / or neohesperidin content of the detected liquid by a made calibration model.

The invention relates to a method for extracting NHDC from the young fruits of Rutaceae plants acid oranges, which comprises, using water-extraction-alcohol-deposition method or alcohol-extraction to abstract the total extractive, extrcting the total extractive with n-butylalcohol to obtain the NHCD mixture, subjecting the mixture to ethanol backflow, recrystallization with water to obtain the high purity NHCD.

The invention provides a medicinal composition mainly containing naringenin, Prunin, hesperetin and hesperetin-7-0-glucoside. A medicine containing naringin, narirutin, hesperidin and neohesperidin isutilized as a raw material, an extract of the medicine is hydrolyzed, or extracting and hydrolyzing are conducted simultaneously on the extract of the medicine, and the plant extract can be obtained.The medicinal composition can be used for development and production of foods, health foods and new traditional Chinese medicines.

The invention provides a quality control method for a medicine for treating a common cold due to wind-cold. The medicine provided by the invention consists of twelve traditional Chinese medicines, such as radix bupleuri, common hogfennel root, ligusticum wallichii, fructus aurantii, notopterygium root, radix angelicae pubescentis, tuckahoe, platycodon grandiflorum, codonopsis pilosula and liquorice, and has the effects of inducing sweat and dispelling exogenous evils, and eliminating wind and clearing dampness. According to the quality control method, the ligusticum wallichii, the fructus aurantii, the radix angelicae pubescentis, the liquorice and mint in the formula are subjected to qualitative identification, and the method has high specificity, reproducibility and durability; naringin, neohesperidin, praeruptorin A and praeruptorin B are subjected to quantitative analyses, and the method has the characteristics of high separation degree, short analysis time, and easiness in operation; the production efficiency can be improved effectively; energy resources can be saved.

The invention provides a method for simultaneously preparing high-purity naringin and neohesperidin from fructus aurantii. Essential oil in the fructus aurantii is extracted by a supercritical CO2 extraction technology at first, and then a naringin and neohesperidin crude extract mixture is extracted by the supercritical CO2 extraction technology. The crude extract mixture is separated and purified by macroporous adsorption resin to obtain the naringin and the neohesperidin. The method provided by the invention has the advantages of high extraction rate, high product purity, environmental friendliness and low cost, and is suitable for industrial production of high-purity naringin and neohesperidin.

The present invention discloses the use of neohesperidin and its composition in preparing gastric power promoting medicine. Neohesperidin and its composition is used in preparing medicines for treating stomach distention, indigestion and infantí»s anorexia.

The invention belongs to the technical field of extraction of plant ingredients, and discloses a method for preparing neohesperidin in physiological fallen fruits of grapefruits by an alcohol / salt double-water-phase system. The method comprises the following steps: collecting dry grapefruit physiological fallen fruits; crushing the dry grapefruit physiological fallen fruits; pulverizing the dry grapefruit physiological fallen fruits; preparing and constructing a double-water-phase system; carrying out hot dip extraction; carrying out separation and phase splitting by a liquid-liquid-solid three-phase disc separating machine; concentrating; carrying out water sedimentation crystallization and filtering; carrying out ultra-filtration film assisted hot water recrystallization purification; carrying out cooling crystallization; and carrying out drying and packing. Advanced processes such as dip extraction through isopropanol / NaH2PO4 inorganic salt double-water-phase system, ultra-filtration film assisted hot water recrystallization purification and the like are adopted, and the whole process has the advantages of short production period, mild extracting conditions, simplicity and convenience in operation, high selectivity to target products, good product quality, small amount of waste water, easiness in treatment and the like, the yield of the prepared neohesperidin reaches 5.7%, and the HPLC content is greater than or equal to 95%.

The invention relates to a detection method of a medicinal preparation of cortex magnoliae officinalis seven-object soup. According to the method, by optimizing an extraction solvent, extraction time and an extraction method, various chemical components in the medicinal preparation of the cortex magnoliae officinalis seven-object soup are furthest extracted; by optimizing an chromatographic column, a mobile phase and an elution mode of the mobile phase, the various chemical components are better separated; thus a method for a fingerprint spectrum of the medicinal preparation is established, and relatively comprehensive spectrum information is acquired; further, by optimally selecting No.8 peak naringin as an internal reference peak, the facts that in common characteristic peaks, a No.9 peak is neohesperidin, a No.14 peak is ammonium glycyrrhetate, a No.15 peak is aloe-emodin, a No.17 peak is rhein, a No.18 peak is emodin, a No.19 peak is honokiol and a No.21 peak is magnolol are affirmed, and relative retention time of 21 common characteristic peaks is determined; by combining information of multiple chromatography peaks in the fingerprint spectrum, the quality can be comprehensively and systematically detected.

The invention relates to a process technology for extracting essential oil and neohesperidin dihydrochalcone from plants, in particular to a preparation process technology for extracting essential oil and neohesperidin dihydrochalcone from orange peels. The process technology is improved and optimized based on a conventional extraction method in the prior art, the steps of ethanol treatment, ultraviolet radiation, macroporous resin adsorption and the like are adopted audaciously, various reaction parameters are combined and optimized effectively, output efficiency of the essential oil and the neohesperidin dihydrochalcone is greatly improved, and the process technology is wide in application prospect.

The invention provides application of neohesperidin in preparing of diabetes preventive treatment compound medicines or health care products. Neohesperidin is extracted and separated from citrus grape fruit tengyuch euonymus bark layers and is subjected to glucose and lipid metabolism biological activity research, and just as naringin, neohesperidin can obviously increase hepatic cell glucose consumption, improve phosphorylation levels of glucose and lipid metabolism related protein adenosine monophosphate activated protein kinase (AMPK) and lipid synthesized key protein activated calcium carbonate (ACC) and cooperate to reduce triglyceride level in lipopexia hepatic cells molded by free fatty acid, has effects of prompting reduction of blood sugar and blood lipid levels, and effect concentration of neohesperidin is far lower than that of positive medicine metformin. Neohesperidin can serve as ancillary drugs or health care products to be used for preventive treatment of diseases related to glucose and lipid metabolism disorder.

The invention provides a method for separating and purifying naringin and neohesperidin from a white skin layer of citrus grandis, which comprises using combining technologies of macroporous resin and high-speed counter-current chromatography, and utilizing drying powder of the white skin layer of the citrus grandis to serve as a raw material, and first preparing macroporous resin crude extract powder; and obtaining the high-purity naringin and neohesperidin by further separation and purification through the high-speed counter-current chromatography. The method is simple to operate, does not need high temperature, high pressure and protection of inert gas and is simple in synthesizing process. A reagent which has strong sharp aroma does not need to be used, and the production process is safe and easy to operate. The method can be used for separating flavonoid compounds which are extremely approximate in using properties and is wide in applicability, short in production cycle and high in purification recovery rate, purity and accuracy. The using amount of samples is little, equipment investment is small, the conditions are stable and easy to control, and the finally purified naringin and the neohesperidin have the purity of over 95% and are suitable for industrial production and scientific research.

The invention discloses a method for preparing hesperetin from enzymatic hydrolysis neohesperidin or hesperidin. Neohesperidin or hesperidin beta-D-glucoside bond can be hydrolyzed in one step through crude enzyme liquid to prepare corresponding aglycone hesperetin. The method includes the steps of preparing the crude enzyme liquid of Penicillium decumbens through fermentation, adjusting the content and reaction time of the crude enzyme liquid, directly converting neohesperidin or hesperidin into hesperetin, dissolving or extracting conversion liquid through organic solvent, and conducting chromatographic purification to obtain hesperetin. By means of the method, the defects that a traditional chemical method is strict in reaction condition, serious in pollution, low in conversion rate and the like when used for preparing hesperetin can be overcome, and the defects that when microbial fermentation is directly adopted, operation is complicated, products are not simplex, and products are difficult to purify can be overcome.