15 results about "Neohesperidin" patented technology

The invention belongs to the technical field of traditional Chinese medicines, and discloses an immature bitter orange-derived extracellular vesicle-like nanoparticle, which is characterized in that the immature bitter orange-derived extracellular vesicle-like nanoparticle is prepared by the steps of crushing immature bitter orange, filtering, centrifuging and resuspending, the average particle size is 60-120 nm, and the particle size of the immature bitter orange-derived extracellular vesicle-like nanoparticle is 20-30 nm. The extracellular vesicle-like nanoparticles are used for entrapment of at least one of naringin, neohesperidin, naringenin and hesperidin. The extracellular vesicle-like nanoparticles derived from immature bitter oranges are simple in preparation method, have liver targeting and good stability, and can be used for preparing medicines for preventing and / or treating liver injury, depression and breast cancer.

This invention relates to a highly active effective fraction of the Epiglottis-Clearing Decoction and its application in the preparation of antitussive drug compositions, belonging to the field of traditional Chinese medicine extract technology. The effective fraction contains hydroxysaffron yellow A, paeoniflorin, naringin, and neohesperidin, with the total content of these four components not less than 20% of the total mass of the effective fraction. The effective fraction of this invention is prepared by purification with macroporous resin. Compared with the freeze-dried powder of the traditional Epiglottis-Clearing Decoction, the total content of the four active ingredients is increased from 3.32% to 20.87%, achieving a more than 6-fold high-efficiency enrichment. Animal pharmacodynamic experiments show that the antitussive effect of a low dose (275.5 mg / kg) of this effective fraction is comparable to that of the freeze-dried powder of the original decoction (1928 mg / kg), reducing the dosage to about 1 / 7 of the original formula, demonstrating a significant "synergistic effect with reduced dosage" technical effect. The effective active ingredients provided by this invention have a clear content and stable quality, and can be made into modern preparations such as tablets, capsules or granules, solving the problems of large volume, inaccurate dosage and poor patient compliance of traditional decoctions.

This invention discloses a method for preparing a new hesperidin, belonging to the field of organic chemical synthesis technology. The method has simple reaction conditions, is safe and environmentally friendly, and can obtain high-purity new hesperidin without purification after the reaction. The method includes the following steps: (1) Take root bark acetylphenyl-4'-β-neohesperidin, add n-butanol, isovalin and 3-indoleacetic acid, and heat to react; (2) After the reaction solution in step (1) has reacted completely, cool down; (3) Filter the cooled solution, wash the solid with n-butanol, and dry to obtain the finished product.

The invention relates to a preparation method of a high-purity neohesperidin dihydrochalcone crystal, which comprises the following steps: dissolving neohesperidin with an alkaline solution, and adding acid and an alcohol solvent for crystallization and purification to obtain high-purity neohesperidin, the method comprises the following steps: reacting neohesperidin in the presence of an alkali solution, hydrogen, palladium on carbon or raney nickel to generate neohesperidin dihydrochalcone, adjusting the pH value, adding alcohol as a mixed solvent, and separating out at normal temperature to obtain high-purity neohesperidin dihydrochalcone; and recrystallizing with alcohol / methyl tertiary ether to obtain the neohesperidin dihydrochalcone crystal. According to the method disclosed by the invention, the problem of low purity of neohesperidin dihydrochalcone in the prior art is solved, and the obtained neohesperidin dihydrochalcone needle-shaped crystal is high in sweetness, long in sweet holding time, high in sweet abundance and long in residual sweet aftertaste.

This invention relates to the technical field of wound care spray formulations, providing a wound repair spray based on herbal extracts and its preparation method. The invention first uses an alkaline alcohol / aqueous solution of *Broomia spp.* leaf extract, neohesperidin, and scopolamine hydrobromide as the extraction system, employing heated ultrasonic extraction. After extraction, dilute HBr is added dropwise to adjust to a weakly acidic state, yielding an extract of *Broomia spp.* leaf extract, neohesperidin extract, and scopolamine hydrobromide extract. This extract is then compounded with extracts of *Bletilla striata*, *Ampelopsis japonica*, *Sanguisorba officinalis*, *Phellodendron chinense*, *Scutellaria baicalensis*, *Polygonum cuspidatum*, and *Platycladus orientalis* leaf extracts to obtain the wound repair spray. During the extraction process, the extract system is stable, homogeneous, clear, and transparent, without precipitation or stratification. The resulting wound repair spray is gentle on the skin and has minimal irritation to wounds. Through the synergistic effect of *Broomia spp.* leaf extract, neohesperidin, and scopolamine hydrobromide, a multi-layered closed loop of antioxidant, anti-inflammatory, blood-activating, and repairing effects is formed. Combined with the addition of natural herbal medicines, it can achieve rapid and efficient wound repair.

The invention provides a construction method of a fingerprint spectrum of Xiangshao granules and the fingerprint spectrum. According to the fingerprint spectrum of the Xiangshao granules provided by the invention, neohesperidin is taken as an internal reference substance, and the relative retention time of the neohesperidin is 1.00, so that the relative retention time of other 26 common peaks in the fingerprint spectrum is determined to be 0.10, 0.11, 0.13, 0.15, 0.35, 0.42, 0.44, 0.48, 0.58, 0.64, 0.71, 0.73, 0.77, 0.79, 0.82, 0.83, 0.91, 0.95, 1.04, 1.18, 1.28, 1.39, 1.44, 1.47, 1.48 and 1.56. Therefore, the obtained fingerprint spectrum can be used for comprehensively and effectively evaluating the quality of the paeonia veitchii granules.

This invention discloses a method for preparing naringin-4'-β-neohesperidin from root bark, belonging to the field of organic chemical synthesis technology. This preparation method can hydrolyze naringin at a lower temperature to generate naringin-4'-β-neohesperidin from root bark. The whole process has few side reactions, high product yield, and less environmental pollution. The method includes the following steps: (1) Dissolving naringin in an ethanol aqueous solution; (2) Adding potassium hydroxide-supported alumina to the solution obtained in step (1) and heating to react; (3) Filtering the reaction solution obtained in step (2) and concentrating the filtrate until there is no ethanol in the solution; (4) Cooling the concentrated solution obtained in step (3) and allowing it to stand to crystallize; (5) Filtering the crystals obtained in step (4), washing with water and drying to obtain the finished product.

A method for improving the solubility of a pharmaceutical compound is disclosed, wherein the pharmaceutical compound is insulin, a natural insulin variant, an insulin variant or insulin analog, a GLP-1 agonist, a GIP agonist, a glucagon agonist, an amylin agonist or an incretin mimic. The method involves using a non-nutritive sugar, such as raspberry glycoside, neohesperidin A, dulcitin B, dodecyl-β-D-maltodextrin (DDM), or carhariside, or any combination thereof, in the presence of a solvent such as ethanol or a mixture thereof, followed by removal of the solvent by drying and re-solubilization to generate a water-soluble complex. The complex formed using the disclosed method is also described.

This invention discloses the application of flavonoids in reducing antibiotic resistance gene pollution in plants. Adding 0.05%–0.2% (by weight of soil) of rutin or neohesperidin to the soil, adjusting soil moisture content, and then planting and cultivating plants for 30 days significantly reduced the abundance of antibiotic resistance genes in the soil and plant leaves compared to the system without rutin or neohesperidin. This alleviates antibiotic resistance gene pollution in the plant cultivation system to a certain extent, resulting in greener and more environmentally friendly plants. It provides a new and effective approach to controlling antibiotic resistance gene pollution in crop cultivation systems, with broad application prospects.

The application discloses a preparation method of a new hesperidine dihydrochalcone-propylene aldehyde adduct, which comprises the following steps: adding new hesperidine dihydrochalcone into methanol to obtain mother liquor A; adding propylene aldehyde into methanol to obtain mother liquor B; mixing the mother liquor A and the mother liquor B, adding phosphate buffer solution, adjusting pH, heating and reacting, and filtering to obtain the new hesperidine dihydrochalcone-propylene aldehyde adduct. The new hesperidine dihydrochalcone-propylene aldehyde adduct prepared by the application can be used as a standard product of a propylene aldehyde reaction product in food detection, and the reaction system can generate the adduct within 5 min, and the clearance rate can reach 95.99% within 40 min. The new hesperidine dihydrochalcone-propylene aldehyde adduct can also be applied to qualitative determination of the propylene aldehyde adduct in rapid food detection. The preparation is simple, low in cost, efficient, free of consumption of a chromogenic agent, high in sample recovery rate, can effectively capture propylene aldehyde, and can control and effectively reduce the content of propylene aldehyde in food.

The invention discloses a flavonoid compound green extraction method based on a deep eutectic solvent, which comprises the following steps: crushing and sieving a qi-tonifying and mass-eliminating formula to obtain compound powder; adding the compound powder and a deep eutectic solvent (DES) into a grinder for MCE grinding extraction to obtain a compound extract; adding the compound extract into pure water, carrying out vortex and centrifugation, and taking supernate, namely a compound extracting solution; according to the method, the DES solvent is used for assisting MCE ball milling to extract the flavonoid components (proanthocyanidin B1, hesperidin, quercetin, aloe-emodin 8-glucoside and neohesperidin) in the formula for tonifying qi and eliminating diseases for the first time, the extraction efficiency is remarkably improved, the extraction process is more environmentally friendly, and the method is suitable for industrial production.

The invention discloses a rhamnosyltransferase mutant as well as a preparation method and application thereof. The enzyme activity of the rhamnosyltransferase mutant disclosed by the invention is respectively improved by 23.67 times and 2.27 times compared with wild type activity data kcat / Km and specific enzyme activity. When the rhamnosyltransferase mutant is applied to preparation of neohesperidin, the rhamnosyl modification efficiency of a reaction substrate can be remarkably improved, then the production efficiency of neohesperidin is improved, and an effective strategy is provided for mass production of neohesperidin.

The application relates to a one-measurement-multiple-evaluation content detection method for Jichuan decoction composition, which comprises the following steps: using an extraction solvent to extract the Jichuan decoction composition to prepare a test sample solution; using a dissolving reagent to dissolve a control sample and naringin to prepare a control sample mixed solution and a naringin solution; respectively sampling the test sample solution, the control sample mixed solution and the naringin solution to perform ultrahigh performance liquid chromatography detection; taking naringin as an internal reference, and calculating the content of the to-be-detected component in the test sample solution according to a formula. The method adopts the one-measurement-multiple-evaluation method taking naringin as the internal reference, obtains the contents of eight components, i.e. caffeic acid, pinitol, ferulic acid, isoferulic acid, verbascoside, naringin, neohesperidin and hesperidin in the Jichuan decoction composition, and has the advantages of high practicability, simple operation, rapid testing, accuracy, cost saving, simple and convenient operation, and the like, and provides a basis for establishing the quality evaluation of the Jichuan decoction composition.

The invention discloses application of isorhamnetin-3-O-neohesperidin in preparation of a medicine for treating nephropathy, and belongs to the technical field of biological medicine. It is found for the first time that isorhamnetin-3-O-neohesperidin has the effect of protecting kidney tissue, pathological injuries of diseased kidneys, side kidneys and renal functions can be effectively relieved, the kidney hemodynamic state of renal fibrosis model mice is improved, and the kidney blood flow is recovered; glomerulus and renal tubule structures are improved, and inflammatory cell infiltration is reduced; renal fibrosis is resisted / inhibited; certain dose dependence is shown when kidney tissue is protected, and the effect is better compared with that of high dose; within the dosage range of effective treatment of renal fibrosis, the pharmaceutical composition has no toxicity to main organs of mice, and has high medication safety. The invention provides a novel and effective medicine for treating kidney diseases.

The present application belongs to the field of preparation of medicinal and edible extract, and particularly relates to a method for improving flavor and reducing bitterness of fructus aurantii immaturi extract by high-pressure radio frequency cold plasma-enzyme coupling. The method comprises the following steps: adding water to fructus aurantii immaturi powder to swell, then adjusting pH, adding hydrolytic enzyme I and complex hydrolytic enzyme to the obtained slurry, and then hydrolyzing at constant temperature, then filtering, and placing the filtrate in a radio frequency cold plasma treatment device; controlling vacuum pressure to be 0.3±0.1 bar, and introducing argon; after radio frequency discharge treatment of the radio frequency cold plasma treatment device, fructus aurantii immaturi extract is obtained. By the method, bitter substances naringin and neohesperidin in fructus aurantii immaturi can be degraded or hydrolyzed, and the content of characteristic aroma components in fructus aurantii immaturi extract can be improved.