The invention relates to the technical field of pharmaceutical analysis, in particular to a method for determining the content of chemical components entering blood of dripping pills with a refreshing
throat. According to the method, a
gas chromatography-
mass spectrometry (GC-MS) technology is adopted, and the chromatographic conditions are as follows: an SH-Stabilwax
capillary column is 30 m * 0.32 mm in specification, and the film thickness is 0.25 mu m; the temperature of a sample inlet is 220 DEG C; the split-flow-free sample injection is realized; the sample size is 1 microliter; the flow of a
chromatographic column is 2mL / min; the
mass spectrum conditions are as follows: the
ion source temperature is 200 DEG C; the interface temperature is 230 DEG C; the
ionization mode is
electron impact ion (EI); the detection
voltage is 0.2 KV; the
solvent is delayed for 2 minutes; and SIM mode. The method creatively realizes the simultaneous determination of the concentrations of 14 volatile components, namely beta-
pinene,
limonene,
eucalyptol, 3-
octanol, isomenthone,
menthone,
camphor,
menthyl acetate, trans-
caryophyllene,
menthol,
borneol, caryophylline, p-cymene and
guaiacol, in the
throat-refreshing dropping
pill or a
drug-containing serum sample after taking the
throat-refreshing dropping
pill. According to the research, accurate monitoring on the
drug effect substance groups in the instant-
swallowing and refreshing containing dropping
pill is realized for the first time, so that not only is a key experimental basis provided for perfecting the
quality control standard, but also a new
view angle is provided for explaining the modern scientific connotation of multi-component synergistic effect of the instant-
swallowing and refreshing containing dropping pill; more scientific data is provided for establishing comprehensive
quality control and quantitative research of the
medicine in the future.